TY  - CHAP
AU  - Davidović, Slobodan
AU  - Aleksić, Jelena
AU  - Stevanović, Milena
AU  - Kovačević-Grujičić, Nataša
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6387
AB  - Mitohondrijska DNK (mtDNK) se odlikuje nizom osobina koje je čine pogodnom za istraživanja evolutivne
istorije ljudskih populacija koja se zasniva na molekularnim markerima ženske linije nasleđivanja. Tokom
poslednje decenije publikovano je više naučnih radova u kojima je analizirana varijabilnost mtDNK u populaciji Srbije primenom markera različite rezolucije uključujući i kompletne genome. U skladu sa očekivanjima zasnovanim na istorijskim, arheološkim i drugim izvorima koji govore u prilog veoma kompleksne istorije populacija na Balkanskom poluostrvu, mtDNK podaci su potvrdili da se srpska populacija odlikuje visokim nivoom raznovrsnosti mtDNK koji je posledica izuzetno složene dinamike ove populacije tokom vremena. Današnji mtDNK profil populacije Srbije ne odstupa od matrilinealnog profila karakterističnog za druge evropske populacije, a genetičke distance pokazuju da ova populacija zauzima centralnu poziciju unutar grupe južnoslovenskih populacija koje se odlikuju visokom heterogenošću. Srpska populacija deli najveći procenat mtDNK haplotipova sa geografski bliskim populacijama Balkanskog poluostrva koje pripadaju južnoslovenskoj grupi, gde su uočeni i potencijalno privatni haplotipovi. Na osnovu filogenetske i filogeografske analize kompletnih mitogenoma u srpskoj populaciji detektovane su retke mtDNK linije,karakteristične za druge regione, poput Bliskog istoka (N1b, HV2), istočne Azije (D4) i Afrike (L2a1), kao i one koje su potencijalno specifične za Balkansko poluostrvo, poput K1a13a1, U4c1b1 i H6a2b. Pored toga, srpska populacija deli određeni broj mtDNK podhaplogrupa sa istočno- i zapadnoslovenskim populacijama kao i sa germanskim populacijama severne i srednje Evrope. Istraživanja varijabilnosti mtDNK su pokazala da se izuzetno velika raznovrsnost mtDNK savremene populacije Srbije može objasniti genetičkim doprinosom kako slovenskih i germanskih, tako i pre-slovenskih populacija koje su naseljavale Balkansko poluostrvo pre Velike seobe naroda.
AB  - The mitochondrial DNA (mtDNA) is characterized by a number of features that make it suitable for study-
ing the evolutionary history of human populations based on molecular markers with the female-specific
line of inheritance. During the last decade, several scientific papers were published in which the mtDNA
variability in the population of Serbia was analyzed using markers of different resolution including com-
plete mitogenomes. In accordance with expectations based on historical, archaeological and other sources
that speak in favor of a very complex history of populations on the Balkan Peninsula, mtDNA data con-
firmed that Serbian population is characterized by a high level of mtDNA diversity, which is a consequence
of the exceptionally complex dynamics of this population over time. Today’s mtDNA profile of the Serbian
population does not differ from the matrilineal landscape characteristic of other European populations,
and according to genetic distances, this population occupies a central position within the group of South-
Slavic populations characterized by high heterogeneity. The Serbian population shares the highest per-
centage of mtDNA haplotypes with the geographically close populations of the Balkan Peninsula
belonging to the South-Slavic group, where potentially private haplotypes were also observed. Phyloge-
netic and phylogeographic analysis of complete mitogenomes in the Serbian population revealed rare
mtDNA lines, characteristic of other regions, such as the Middle East (N1b, HV2), East Asia (D4) and Africa
(L2a1), as well as those that are potentially specific for Balkan Peninsula, like K1a13a1, U4c1b1 and H6a2b.
In addition, Serbian population shares a certain number of mtDNA subhaplogroups with East- and West-
Slavic populations as well as with the Germanic populations of Northern and Central Europe. Studies of
mtDNA variability have shown that the exceptionally high mtDNA diversity in contemporary Serbian pop-
ulation may be associated with the genetic contribution of both Slavic and Germanic, as well as pre-Slavic
populations that inhabited the Balkan Peninsula before the Great Migration.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
T2  - Trends in Molecular Biology
T1  - Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije
T1  - Mitochondrial gene pool variability of the residents of the Republic of Serbia
IS  - 3
SP  - 18
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6387
ER  - 

@inbook{
author = "Davidović, Slobodan and Aleksić, Jelena and Stevanović, Milena and Kovačević-Grujičić, Nataša",
year = "2023",
abstract = "Mitohondrijska DNK (mtDNK) se odlikuje nizom osobina koje je čine pogodnom za istraživanja evolutivne
istorije ljudskih populacija koja se zasniva na molekularnim markerima ženske linije nasleđivanja. Tokom
poslednje decenije publikovano je više naučnih radova u kojima je analizirana varijabilnost mtDNK u populaciji Srbije primenom markera različite rezolucije uključujući i kompletne genome. U skladu sa očekivanjima zasnovanim na istorijskim, arheološkim i drugim izvorima koji govore u prilog veoma kompleksne istorije populacija na Balkanskom poluostrvu, mtDNK podaci su potvrdili da se srpska populacija odlikuje visokim nivoom raznovrsnosti mtDNK koji je posledica izuzetno složene dinamike ove populacije tokom vremena. Današnji mtDNK profil populacije Srbije ne odstupa od matrilinealnog profila karakterističnog za druge evropske populacije, a genetičke distance pokazuju da ova populacija zauzima centralnu poziciju unutar grupe južnoslovenskih populacija koje se odlikuju visokom heterogenošću. Srpska populacija deli najveći procenat mtDNK haplotipova sa geografski bliskim populacijama Balkanskog poluostrva koje pripadaju južnoslovenskoj grupi, gde su uočeni i potencijalno privatni haplotipovi. Na osnovu filogenetske i filogeografske analize kompletnih mitogenoma u srpskoj populaciji detektovane su retke mtDNK linije,karakteristične za druge regione, poput Bliskog istoka (N1b, HV2), istočne Azije (D4) i Afrike (L2a1), kao i one koje su potencijalno specifične za Balkansko poluostrvo, poput K1a13a1, U4c1b1 i H6a2b. Pored toga, srpska populacija deli određeni broj mtDNK podhaplogrupa sa istočno- i zapadnoslovenskim populacijama kao i sa germanskim populacijama severne i srednje Evrope. Istraživanja varijabilnosti mtDNK su pokazala da se izuzetno velika raznovrsnost mtDNK savremene populacije Srbije može objasniti genetičkim doprinosom kako slovenskih i germanskih, tako i pre-slovenskih populacija koje su naseljavale Balkansko poluostrvo pre Velike seobe naroda., The mitochondrial DNA (mtDNA) is characterized by a number of features that make it suitable for study-
ing the evolutionary history of human populations based on molecular markers with the female-specific
line of inheritance. During the last decade, several scientific papers were published in which the mtDNA
variability in the population of Serbia was analyzed using markers of different resolution including com-
plete mitogenomes. In accordance with expectations based on historical, archaeological and other sources
that speak in favor of a very complex history of populations on the Balkan Peninsula, mtDNA data con-
firmed that Serbian population is characterized by a high level of mtDNA diversity, which is a consequence
of the exceptionally complex dynamics of this population over time. Today’s mtDNA profile of the Serbian
population does not differ from the matrilineal landscape characteristic of other European populations,
and according to genetic distances, this population occupies a central position within the group of South-
Slavic populations characterized by high heterogeneity. The Serbian population shares the highest per-
centage of mtDNA haplotypes with the geographically close populations of the Balkan Peninsula
belonging to the South-Slavic group, where potentially private haplotypes were also observed. Phyloge-
netic and phylogeographic analysis of complete mitogenomes in the Serbian population revealed rare
mtDNA lines, characteristic of other regions, such as the Middle East (N1b, HV2), East Asia (D4) and Africa
(L2a1), as well as those that are potentially specific for Balkan Peninsula, like K1a13a1, U4c1b1 and H6a2b.
In addition, Serbian population shares a certain number of mtDNA subhaplogroups with East- and West-
Slavic populations as well as with the Germanic populations of Northern and Central Europe. Studies of
mtDNA variability have shown that the exceptionally high mtDNA diversity in contemporary Serbian pop-
ulation may be associated with the genetic contribution of both Slavic and Germanic, as well as pre-Slavic
populations that inhabited the Balkan Peninsula before the Great Migration.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Trends in Molecular Biology",
booktitle = "Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije, Mitochondrial gene pool variability of the residents of the Republic of Serbia",
number = "3",
pages = "18-36",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6387"
}

Davidović, S., Aleksić, J., Stevanović, M.,& Kovačević-Grujičić, N.. (2023). Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije. in Trends in Molecular Biology
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade.(3), 18-36.
https://hdl.handle.net/21.15107/rcub_ibiss_6387

Davidović S, Aleksić J, Stevanović M, Kovačević-Grujičić N. Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije. in Trends in Molecular Biology. 2023;(3):18-36.
https://hdl.handle.net/21.15107/rcub_ibiss_6387 .

Davidović, Slobodan, Aleksić, Jelena, Stevanović, Milena, Kovačević-Grujičić, Nataša, "Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije" in Trends in Molecular Biology, no. 3 (2023):18-36,
https://hdl.handle.net/21.15107/rcub_ibiss_6387 .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Marinković, Saša
AU  - Hribšek, Irena
AU  - Popić, Vuk
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6416
AB  - Genetic diversity is highlighted by IUCN as one of the three pillars of biodiversity that need to be protected together with species and ecosystem diversity. Thus, before any conservation measures of reintroduction/restocking are undertaken, especially in the regions where autochthonous populations exist, it is necessary to evaluate the genetic diversity of both the autochthonous and introducing populations. If there are no autochthonous populations, the nearest neighbouring populations are used as donors and only if neighbouring populations are non-existent distant populations could be used. In this way, the local adaptations and genetic variation specific to the region will be preserved.
In the Balkan Peninsula, the stable and autochthonous population of Griffon vulture persists and it should be protected as such. The genetic data based on microsatellites demonstrated the existence of two genetic clusters one of which is specific for the Balkan and Iberian Peninsula. The study of mitochondrial DNA diversity demonstrated the existence of private haplotypes unique for both peninsulas. Long-time monitoring of named populations demonstrated different migration patterns. Birds from the Balkans migrate almost exclusively to the Middle East while the Iberian birds migrate to northwest Africa, which is the reason why they rarely come into contact. Natal philopatric behaviour specific to the species dictates that the unique genetic fingerprint will remain fixed in the region of their origin. Thus, in nature, gene flow between named populations is low, or insignificant. In addition, somewhat different climate conditions between Iberian and Balkan peninsula could also drive genetic differentiation due to the local adaptations.
The detected level of genetic differentiation between these regions suggests that different conservation approaches are needed to preserve specific and unique genetic diversity and that the current program of restocking the vulture population of the Balkan Peninsula with the birds from the Iberian Peninsula needs to be re-evaluated.
PB  - Caceres: Vulture Conservation Foundation
C3  - Abstract book: European Vulture Conference: Vultures 2023; 2023 Nov 14-17; Caceres, Spain
T1  - Using genetics for creating proper conservation strategies for protected species – the case of Griffon Vulture (Gyps fulvus)
SP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6416
ER  - 

@conference{
author = "Davidović, Slobodan and Marinković, Saša and Hribšek, Irena and Popić, Vuk and Erić, Pavle and Patenković, Aleksandra and Tanasković, Marija",
year = "2023",
abstract = "Genetic diversity is highlighted by IUCN as one of the three pillars of biodiversity that need to be protected together with species and ecosystem diversity. Thus, before any conservation measures of reintroduction/restocking are undertaken, especially in the regions where autochthonous populations exist, it is necessary to evaluate the genetic diversity of both the autochthonous and introducing populations. If there are no autochthonous populations, the nearest neighbouring populations are used as donors and only if neighbouring populations are non-existent distant populations could be used. In this way, the local adaptations and genetic variation specific to the region will be preserved.
In the Balkan Peninsula, the stable and autochthonous population of Griffon vulture persists and it should be protected as such. The genetic data based on microsatellites demonstrated the existence of two genetic clusters one of which is specific for the Balkan and Iberian Peninsula. The study of mitochondrial DNA diversity demonstrated the existence of private haplotypes unique for both peninsulas. Long-time monitoring of named populations demonstrated different migration patterns. Birds from the Balkans migrate almost exclusively to the Middle East while the Iberian birds migrate to northwest Africa, which is the reason why they rarely come into contact. Natal philopatric behaviour specific to the species dictates that the unique genetic fingerprint will remain fixed in the region of their origin. Thus, in nature, gene flow between named populations is low, or insignificant. In addition, somewhat different climate conditions between Iberian and Balkan peninsula could also drive genetic differentiation due to the local adaptations.
The detected level of genetic differentiation between these regions suggests that different conservation approaches are needed to preserve specific and unique genetic diversity and that the current program of restocking the vulture population of the Balkan Peninsula with the birds from the Iberian Peninsula needs to be re-evaluated.",
publisher = "Caceres: Vulture Conservation Foundation",
journal = "Abstract book: European Vulture Conference: Vultures 2023; 2023 Nov 14-17; Caceres, Spain",
title = "Using genetics for creating proper conservation strategies for protected species – the case of Griffon Vulture (Gyps fulvus)",
pages = "54",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6416"
}

Davidović, S., Marinković, S., Hribšek, I., Popić, V., Erić, P., Patenković, A.,& Tanasković, M.. (2023). Using genetics for creating proper conservation strategies for protected species – the case of Griffon Vulture (Gyps fulvus). in Abstract book: European Vulture Conference: Vultures 2023; 2023 Nov 14-17; Caceres, Spain
Caceres: Vulture Conservation Foundation., 54.
https://hdl.handle.net/21.15107/rcub_ibiss_6416

Davidović S, Marinković S, Hribšek I, Popić V, Erić P, Patenković A, Tanasković M. Using genetics for creating proper conservation strategies for protected species – the case of Griffon Vulture (Gyps fulvus). in Abstract book: European Vulture Conference: Vultures 2023; 2023 Nov 14-17; Caceres, Spain. 2023;:54.
https://hdl.handle.net/21.15107/rcub_ibiss_6416 .

Davidović, Slobodan, Marinković, Saša, Hribšek, Irena, Popić, Vuk, Erić, Pavle, Patenković, Aleksandra, Tanasković, Marija, "Using genetics for creating proper conservation strategies for protected species – the case of Griffon Vulture (Gyps fulvus)" in Abstract book: European Vulture Conference: Vultures 2023; 2023 Nov 14-17; Caceres, Spain (2023):54,
https://hdl.handle.net/21.15107/rcub_ibiss_6416 .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Erić, Pavle
AU  - Tanasković, Marija
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6388
AB  - Honey bees (Apis mellifera) and bumblebees (Bombus terrestris) are pollinators that cover large areas during their foraging activities. As a result, they encounter various environments and pollutants, making them an ideal choice as bioindicators for environmental pollution. The town of Bor in eastern Serbia houses the largest mine for copper, gold, and silver extraction, and is crucial to regularly monitor the environmental impact of mining activities.
The presence and concentration of heavy metals (Fe, Cu, Zn, Cd, Li, Pb, Au, Ni) were measured using the ICP OES method for samples of both species at six locations in the basin of the Bor mining facility and one in the remote region. Results indicate that both species accumulate heavy metals in their bodies during foraging activities in similar manner. Elevated  concentrations of Fe, Zn, Cd and Pb were detected in both species in the same localities while Cu, Li and Ni were variable depending on the locality. Interestingly the Cu concentration in A. mellifera was less variable than in B. terrestris indicating different accumulation pattern, possibly species specific.
Both pollinator species serve as potential bioindicators for environmental pollution in the area, reflecting ecosystem health and heavy metal infiltration in the food chain.
PB  - Toronto, Canada: York University
C3  - BeeCon 2023; 2023 Oct 12-13; Toronto, Canada
T1  - Two different pollinator species Apis mellifera and Bombus terrestris as indicators of heavy metal pollution near the mining complex in Bor, Serbia
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6388
ER  - 

@conference{
author = "Davidović, Slobodan and Patenković, Aleksandra and Erić, Katarina and Erić, Pavle and Tanasković, Marija",
year = "2023",
abstract = "Honey bees (Apis mellifera) and bumblebees (Bombus terrestris) are pollinators that cover large areas during their foraging activities. As a result, they encounter various environments and pollutants, making them an ideal choice as bioindicators for environmental pollution. The town of Bor in eastern Serbia houses the largest mine for copper, gold, and silver extraction, and is crucial to regularly monitor the environmental impact of mining activities.
The presence and concentration of heavy metals (Fe, Cu, Zn, Cd, Li, Pb, Au, Ni) were measured using the ICP OES method for samples of both species at six locations in the basin of the Bor mining facility and one in the remote region. Results indicate that both species accumulate heavy metals in their bodies during foraging activities in similar manner. Elevated  concentrations of Fe, Zn, Cd and Pb were detected in both species in the same localities while Cu, Li and Ni were variable depending on the locality. Interestingly the Cu concentration in A. mellifera was less variable than in B. terrestris indicating different accumulation pattern, possibly species specific.
Both pollinator species serve as potential bioindicators for environmental pollution in the area, reflecting ecosystem health and heavy metal infiltration in the food chain.",
publisher = "Toronto, Canada: York University",
journal = "BeeCon 2023; 2023 Oct 12-13; Toronto, Canada",
title = "Two different pollinator species Apis mellifera and Bombus terrestris as indicators of heavy metal pollution near the mining complex in Bor, Serbia",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6388"
}

Davidović, S., Patenković, A., Erić, K., Erić, P.,& Tanasković, M.. (2023). Two different pollinator species Apis mellifera and Bombus terrestris as indicators of heavy metal pollution near the mining complex in Bor, Serbia. in BeeCon 2023; 2023 Oct 12-13; Toronto, Canada
Toronto, Canada: York University., 8.
https://hdl.handle.net/21.15107/rcub_ibiss_6388

Davidović S, Patenković A, Erić K, Erić P, Tanasković M. Two different pollinator species Apis mellifera and Bombus terrestris as indicators of heavy metal pollution near the mining complex in Bor, Serbia. in BeeCon 2023; 2023 Oct 12-13; Toronto, Canada. 2023;:8.
https://hdl.handle.net/21.15107/rcub_ibiss_6388 .

Davidović, Slobodan, Patenković, Aleksandra, Erić, Katarina, Erić, Pavle, Tanasković, Marija, "Two different pollinator species Apis mellifera and Bombus terrestris as indicators of heavy metal pollution near the mining complex in Bor, Serbia" in BeeCon 2023; 2023 Oct 12-13; Toronto, Canada (2023):8,
https://hdl.handle.net/21.15107/rcub_ibiss_6388 .

TY  - JOUR
AU  - Kloska, Anna
AU  - Giełczyk, Agata
AU  - Grzybowski, Tomasz
AU  - Płoski, Rafał
AU  - Kloska, Sylwester
AU  - Marciniak, Tomasz
AU  - Pałczynski, Krzysztof
AU  - Rogalla-Ładniak, Urszula
AU  - Malyarchuk, Boris
AU  - Derenko, Miroslava
AU  - Kovačević-Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
AU  - Davidović, Slobodan
AU  - Spólnicka, Magdalena
AU  - Zubanska, Magdalena
AU  - Wozniak, Marcin
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6321
AB  - Abstract

Data obtained with the use of massive parallel sequencing (MPS) can be valuable in population genetics studies. In particular, such data harbor the potential for distinguishing samples from different populations, especially from those coming from adjacent populations of common origin. Machine learning (ML) techniques seem to be especially well suited for analyzing large datasets obtained using MPS. The Slavic populations constitute about a third of the population of Europe and inhabit a large area of the continent, while being relatively closely related in population genetics terms. In this proof-of-concept study, various ML techniques were used to classify DNA samples from Slavic and non-Slavic individuals. The primary objective of this study was to empirically evaluate the feasibility of discerning the genetic provenance of individuals of Slavic descent who exhibit genetic similarity, with the overarching goal of categorizing DNA specimens derived from diverse Slavic population representatives. Raw sequencing data were pre-processed, to obtain a 1200 character-long binary vector. A total of three classifiers were used—Random Forest, Support Vector Machine (SVM), and XGBoost. The most-promising results were obtained using SVM with a linear kernel, with 99.9% accuracy and F1-scores of 0.9846–1.000 for all classes.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe
IS  - 20
VL  - 24
DO  - 10.3390/ijms242015095
SP  - 15095
ER  - 

@article{
author = "Kloska, Anna and Giełczyk, Agata and Grzybowski, Tomasz and Płoski, Rafał and Kloska, Sylwester and Marciniak, Tomasz and Pałczynski, Krzysztof and Rogalla-Ładniak, Urszula and Malyarchuk, Boris and Derenko, Miroslava and Kovačević-Grujičić, Nataša and Stevanović, Milena and Drakulić, Danijela and Davidović, Slobodan and Spólnicka, Magdalena and Zubanska, Magdalena and Wozniak, Marcin",
year = "2023",
abstract = "Abstract

Data obtained with the use of massive parallel sequencing (MPS) can be valuable in population genetics studies. In particular, such data harbor the potential for distinguishing samples from different populations, especially from those coming from adjacent populations of common origin. Machine learning (ML) techniques seem to be especially well suited for analyzing large datasets obtained using MPS. The Slavic populations constitute about a third of the population of Europe and inhabit a large area of the continent, while being relatively closely related in population genetics terms. In this proof-of-concept study, various ML techniques were used to classify DNA samples from Slavic and non-Slavic individuals. The primary objective of this study was to empirically evaluate the feasibility of discerning the genetic provenance of individuals of Slavic descent who exhibit genetic similarity, with the overarching goal of categorizing DNA specimens derived from diverse Slavic population representatives. Raw sequencing data were pre-processed, to obtain a 1200 character-long binary vector. A total of three classifiers were used—Random Forest, Support Vector Machine (SVM), and XGBoost. The most-promising results were obtained using SVM with a linear kernel, with 99.9% accuracy and F1-scores of 0.9846–1.000 for all classes.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe",
number = "20",
volume = "24",
doi = "10.3390/ijms242015095",
pages = "15095"
}

Kloska, A., Giełczyk, A., Grzybowski, T., Płoski, R., Kloska, S., Marciniak, T., Pałczynski, K., Rogalla-Ładniak, U., Malyarchuk, B., Derenko, M., Kovačević-Grujičić, N., Stevanović, M., Drakulić, D., Davidović, S., Spólnicka, M., Zubanska, M.,& Wozniak, M.. (2023). A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe. in International Journal of Molecular Sciences
Basel: MDPI., 24(20), 15095.
https://doi.org/10.3390/ijms242015095

Kloska A, Giełczyk A, Grzybowski T, Płoski R, Kloska S, Marciniak T, Pałczynski K, Rogalla-Ładniak U, Malyarchuk B, Derenko M, Kovačević-Grujičić N, Stevanović M, Drakulić D, Davidović S, Spólnicka M, Zubanska M, Wozniak M. A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe. in International Journal of Molecular Sciences. 2023;24(20):15095.
doi:10.3390/ijms242015095 .

Kloska, Anna, Giełczyk, Agata, Grzybowski, Tomasz, Płoski, Rafał, Kloska, Sylwester, Marciniak, Tomasz, Pałczynski, Krzysztof, Rogalla-Ładniak, Urszula, Malyarchuk, Boris, Derenko, Miroslava, Kovačević-Grujičić, Nataša, Stevanović, Milena, Drakulić, Danijela, Davidović, Slobodan, Spólnicka, Magdalena, Zubanska, Magdalena, Wozniak, Marcin, "A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe" in International Journal of Molecular Sciences, 24, no. 20 (2023):15095,
https://doi.org/10.3390/ijms242015095 . .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Stanković, Milica
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5868
AB  - White-tailed eagle is the biggest bird of prey in Central and Southeast Europe. In Serbia it inhabits the Vojvodina province and the valleys of Danube, Sava, Tisa and Tamiš. Anthropogenic pressure on its habitats in Europe caused a decline in its numbers, but due to the strict laws protecting both species and its habitats, birds’ numbers are now steady and increasing. In Serbia, as a strictly protected species it is a subject of different conservation programs. The available genetic data for this population are scarce and it is necessary to assess its genetic diversity to improve the existing conservation efforts. ND1 and Cytb mitochondrial genes can be used to estimate the populations’ adaptation to different environmental conditions and their variability can potentially be used to evaluate differentiation between populations.
To assess the genetic diversity of White-tailed eagle in Serbia we used mitochondrial ND1 and Cytb nucleotide sequences from 40 unrelated birds collected in nests. ND1 and Cytb nucleotide sequences variability was evaluated using standard parameters of genetic diversity (PGD). Acquired values were compared with the available data for the variability of the D-loop region which showed that combined ND1/Cytb nucleotide sequences PGD provide comparable results. Using publicly available sequences we reconstructed haplotype networks for ND1, Cytb, ND1/Cytb and D-loop which further showed the applicability of ND1/Cytb in population genetics analyses. Phylogeny reconstructed using combined ND1/Cytb sequences identified two branches in Serbian white-tailed eagles. Although the majority of substitutions were nonsynonymous, no selective pressure was detected.
Our data suggest that combined ND1/Cytb sequence variability provides sufficient information to be used for population comparison, population differentiation analyses and phylogeny reconstruction, but also gives a tool to potentially identify adaptations to different environmental conditions.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
C3  - Book of Abstracts: 4th Belgrade Bioinformatics Conference: BELBI2023; 2023 Jun 1-23; Belgrade, Serbia
T1  - Evaluating ND1 and Cytb mitochondrial genes as markers for diversity analysis of protected White-tailed eagle species from Serbia
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5868
ER  - 

@conference{
editor = "Morić, Ivana, Đorđević, Valentina",
author = "Davidović, Slobodan and Stanković, Milica and Erić, Pavle and Erić, Katarina and Patenković, Aleksandra and Tanasković, Marija",
year = "2023",
abstract = "White-tailed eagle is the biggest bird of prey in Central and Southeast Europe. In Serbia it inhabits the Vojvodina province and the valleys of Danube, Sava, Tisa and Tamiš. Anthropogenic pressure on its habitats in Europe caused a decline in its numbers, but due to the strict laws protecting both species and its habitats, birds’ numbers are now steady and increasing. In Serbia, as a strictly protected species it is a subject of different conservation programs. The available genetic data for this population are scarce and it is necessary to assess its genetic diversity to improve the existing conservation efforts. ND1 and Cytb mitochondrial genes can be used to estimate the populations’ adaptation to different environmental conditions and their variability can potentially be used to evaluate differentiation between populations.
To assess the genetic diversity of White-tailed eagle in Serbia we used mitochondrial ND1 and Cytb nucleotide sequences from 40 unrelated birds collected in nests. ND1 and Cytb nucleotide sequences variability was evaluated using standard parameters of genetic diversity (PGD). Acquired values were compared with the available data for the variability of the D-loop region which showed that combined ND1/Cytb nucleotide sequences PGD provide comparable results. Using publicly available sequences we reconstructed haplotype networks for ND1, Cytb, ND1/Cytb and D-loop which further showed the applicability of ND1/Cytb in population genetics analyses. Phylogeny reconstructed using combined ND1/Cytb sequences identified two branches in Serbian white-tailed eagles. Although the majority of substitutions were nonsynonymous, no selective pressure was detected.
Our data suggest that combined ND1/Cytb sequence variability provides sufficient information to be used for population comparison, population differentiation analyses and phylogeny reconstruction, but also gives a tool to potentially identify adaptations to different environmental conditions.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Book of Abstracts: 4th Belgrade Bioinformatics Conference: BELBI2023; 2023 Jun 1-23; Belgrade, Serbia",
title = "Evaluating ND1 and Cytb mitochondrial genes as markers for diversity analysis of protected White-tailed eagle species from Serbia",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5868"
}

Morić, I., Đorđević, V., Davidović, S., Stanković, M., Erić, P., Erić, K., Patenković, A.,& Tanasković, M.. (2023). Evaluating ND1 and Cytb mitochondrial genes as markers for diversity analysis of protected White-tailed eagle species from Serbia. in Book of Abstracts: 4th Belgrade Bioinformatics Conference: BELBI2023; 2023 Jun 1-23; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5868

Morić I, Đorđević V, Davidović S, Stanković M, Erić P, Erić K, Patenković A, Tanasković M. Evaluating ND1 and Cytb mitochondrial genes as markers for diversity analysis of protected White-tailed eagle species from Serbia. in Book of Abstracts: 4th Belgrade Bioinformatics Conference: BELBI2023; 2023 Jun 1-23; Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5868 .

Morić, Ivana, Đorđević, Valentina, Davidović, Slobodan, Stanković, Milica, Erić, Pavle, Erić, Katarina, Patenković, Aleksandra, Tanasković, Marija, "Evaluating ND1 and Cytb mitochondrial genes as markers for diversity analysis of protected White-tailed eagle species from Serbia" in Book of Abstracts: 4th Belgrade Bioinformatics Conference: BELBI2023; 2023 Jun 1-23; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5868 .

TY  - JOUR
AU  - Erić, Katarina
AU  - Savić Veselinović, Marija
AU  - Patenković, Aleksandra
AU  - Davidović, Slobodan
AU  - Erić, Pavle
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5865
AB  - Drosophila subobscura is considered a good model species for investigation of a population’s ability to adapt and cope with climate changes. Decade long research has shown that inversion frequencies change in response to environmental factors indicating their role in adaptation to novel environments. The mechanisms behind organisms’ responses to temperature are complex, involving changes in physiology, behavior, gene expression and regulation. On the other hand, a population’s ability to respond to suboptimal conditions depends on standing genetic variation and population history. In order to elucidate the role of local adaptation in population response to the changing temperature, we investigated the response to temperature in D. subobscura individuals originating from two different altitudes by combining traditional cytogenetic techniques with assessing the levels of Hsp70 protein expression. Inversion polymorphism was assessed in the flies sampled from natural populations and in flies reared in laboratory conditions at three different temperatures after five and sixteen generations and Hsp70 protein expression profile in 12th generation flies at the basal level and after heat shock induction. Our results indicate that local adaptation and population history influence population response to the changing temperature.
PB  - Basel: MDPI
T2  - Life
T1  - Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura
IS  - 6
VL  - 13
DO  - 10.3390/life13061333
SP  - 1333
ER  - 

@article{
author = "Erić, Katarina and Savić Veselinović, Marija and Patenković, Aleksandra and Davidović, Slobodan and Erić, Pavle and Stamenković-Radak, Marina and Tanasković, Marija",
year = "2023",
abstract = "Drosophila subobscura is considered a good model species for investigation of a population’s ability to adapt and cope with climate changes. Decade long research has shown that inversion frequencies change in response to environmental factors indicating their role in adaptation to novel environments. The mechanisms behind organisms’ responses to temperature are complex, involving changes in physiology, behavior, gene expression and regulation. On the other hand, a population’s ability to respond to suboptimal conditions depends on standing genetic variation and population history. In order to elucidate the role of local adaptation in population response to the changing temperature, we investigated the response to temperature in D. subobscura individuals originating from two different altitudes by combining traditional cytogenetic techniques with assessing the levels of Hsp70 protein expression. Inversion polymorphism was assessed in the flies sampled from natural populations and in flies reared in laboratory conditions at three different temperatures after five and sixteen generations and Hsp70 protein expression profile in 12th generation flies at the basal level and after heat shock induction. Our results indicate that local adaptation and population history influence population response to the changing temperature.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura",
number = "6",
volume = "13",
doi = "10.3390/life13061333",
pages = "1333"
}

Erić, K., Savić Veselinović, M., Patenković, A., Davidović, S., Erić, P., Stamenković-Radak, M.,& Tanasković, M.. (2023). Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura. in Life
Basel: MDPI., 13(6), 1333.
https://doi.org/10.3390/life13061333

Erić K, Savić Veselinović M, Patenković A, Davidović S, Erić P, Stamenković-Radak M, Tanasković M. Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura. in Life. 2023;13(6):1333.
doi:10.3390/life13061333 .

Erić, Katarina, Savić Veselinović, Marija, Patenković, Aleksandra, Davidović, Slobodan, Erić, Pavle, Stamenković-Radak, Marina, Tanasković, Marija, "Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura" in Life, 13, no. 6 (2023):1333,
https://doi.org/10.3390/life13061333 . .

TY  - JOUR
AU  - Davidović, Slobodan
AU  - Marinković, Saša
AU  - Kukobat, Mila
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Hribšek, Irena
AU  - Tanasković, Marija
AU  - Stamenković-Radak, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4751
AB  - Once a widespread and common species across the region of southeast Europe, the Griffon vulture is now confined to small and isolated populations across the Balkan Peninsula. The population from Serbia with 290 couples represents its biggest and most viable population that can serve as an important reservoir of genetic diversity from which the birds can be used for the region’s reintroduction or recolonization programs. To estimate the level of genetic diversity, the mitochondrial Cytb gene from 58 unrelated birds sampled during the marking in the nests was sequenced and compared to the homologous Griffon vulture sequences available in publicly accessible online databases. Phylogeographic analysis based on Cytb sequences showed that the most frequent haplotype is found in all Griffon vulture populations and that each population possesses private haplotypes. Our data suggest that the Griffon vulture population from Serbia should be used as a source population for restocking and reintroduction programs in the region. The observed genetic differentiation between the populations from the Iberian and Balkan Peninsulas suggest that the introduction of foreign birds from remote populations should be avoided and that birds from indigenous or neighboring populations, if available, should be used instead.
PB  - Basel: MDPI
T2  - Life
T1  - Genetic Diversity Analysis of Mitochondrial Cytb Gene, Phylogeny and Phylogeography of Protected Griffon Vulture (Gyps fulvus) from Serbia
IS  - 2
VL  - 12
DO  - 10.3390/life12020164
SP  - 164
ER  - 

@article{
author = "Davidović, Slobodan and Marinković, Saša and Kukobat, Mila and Mihajlović, Milica and Tanasić, Vanja and Hribšek, Irena and Tanasković, Marija and Stamenković-Radak, Marina",
year = "2022",
abstract = "Once a widespread and common species across the region of southeast Europe, the Griffon vulture is now confined to small and isolated populations across the Balkan Peninsula. The population from Serbia with 290 couples represents its biggest and most viable population that can serve as an important reservoir of genetic diversity from which the birds can be used for the region’s reintroduction or recolonization programs. To estimate the level of genetic diversity, the mitochondrial Cytb gene from 58 unrelated birds sampled during the marking in the nests was sequenced and compared to the homologous Griffon vulture sequences available in publicly accessible online databases. Phylogeographic analysis based on Cytb sequences showed that the most frequent haplotype is found in all Griffon vulture populations and that each population possesses private haplotypes. Our data suggest that the Griffon vulture population from Serbia should be used as a source population for restocking and reintroduction programs in the region. The observed genetic differentiation between the populations from the Iberian and Balkan Peninsulas suggest that the introduction of foreign birds from remote populations should be avoided and that birds from indigenous or neighboring populations, if available, should be used instead.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Genetic Diversity Analysis of Mitochondrial Cytb Gene, Phylogeny and Phylogeography of Protected Griffon Vulture (Gyps fulvus) from Serbia",
number = "2",
volume = "12",
doi = "10.3390/life12020164",
pages = "164"
}

Davidović, S., Marinković, S., Kukobat, M., Mihajlović, M., Tanasić, V., Hribšek, I., Tanasković, M.,& Stamenković-Radak, M.. (2022). Genetic Diversity Analysis of Mitochondrial Cytb Gene, Phylogeny and Phylogeography of Protected Griffon Vulture (Gyps fulvus) from Serbia. in Life
Basel: MDPI., 12(2), 164.
https://doi.org/10.3390/life12020164

Davidović S, Marinković S, Kukobat M, Mihajlović M, Tanasić V, Hribšek I, Tanasković M, Stamenković-Radak M. Genetic Diversity Analysis of Mitochondrial Cytb Gene, Phylogeny and Phylogeography of Protected Griffon Vulture (Gyps fulvus) from Serbia. in Life. 2022;12(2):164.
doi:10.3390/life12020164 .

Davidović, Slobodan, Marinković, Saša, Kukobat, Mila, Mihajlović, Milica, Tanasić, Vanja, Hribšek, Irena, Tanasković, Marija, Stamenković-Radak, Marina, "Genetic Diversity Analysis of Mitochondrial Cytb Gene, Phylogeny and Phylogeography of Protected Griffon Vulture (Gyps fulvus) from Serbia" in Life, 12, no. 2 (2022):164,
https://doi.org/10.3390/life12020164 . .

TY  - JOUR
AU  - Davidović, Slobodan
AU  - Marinković, Saša
AU  - Hribšek, Irena
AU  - Patenković, Aleksandra
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5304
AB  - Background: Once a widespread species across the region of Southeast Europe, the
Griffon vulture is now confined to small and isolated populations across the Balkan
Peninsula. The population from Serbia represents its biggest and most viable
population that can serve as an important reservoir of genetic diversity from which
the birds can be used for the region’s reintroduction programmes. The available
genetic data for this valuable population are scarce and as a protected species that
belongs to the highly endangered vulture group, it needs to be well described so that it
can be properly managed and used as a restocking population. Considering the
serious recent bottleneck event that the Griffon vulture population from Serbia
experienced we estimated the overall relatedness among the birds from this
population. Sex ratio, another important parameter that shows the vitality and
strength of the population was evaluated as well.
Methods: During the annual monitoring that was performed in the period from
2013–2021, we collected blood samples from individual birds that were marked in the
nests. In total, 169 samples were collected and each was used for molecular sexing
while 58 presumably unrelated birds from different nests were used for inbreeding
and relatedness analyses. The relatedness was estimated using both biparentally (10
microsatellite loci) and uniparentally (Cytb and D-loop I of mitochondrial DNA)
inherited markers.
Results: The level of inbreeding was relatively high and on average it was 8.3% while
the mean number of relatives for each bird was close to three. The sex ratio was close
to 1:1 and for the analysed period of 9 years, it didn’t demonstrate a statistically
significant deviation from the expected ratio of 1:1, suggesting that this is a stable and
healthy population. Our data suggest that, even though a relatively high level of
inbreeding can be detected among the individual birds, the Griffon vulture
population from Serbia can be used as a source population for restocking and
reintroduction programmes in the region. These data combined with previously
observed genetic differentiation between the populations from the Iberian and
Balkan Peninsulas suggest that the introduction of foreign birds should be avoided
and that local birds should be used instead.
PB  - PeerJ
T2  - PeerJ
T1  - Sex ratio and relatedness in the Griffon vulture (Gyps fulvus) population of Serbia
VL  - 10
DO  - 10.7717/peerj.14477
SP  - e14477
ER  - 

@article{
author = "Davidović, Slobodan and Marinković, Saša and Hribšek, Irena and Patenković, Aleksandra and Stamenković-Radak, Marina and Tanasković, Marija",
year = "2022",
abstract = "Background: Once a widespread species across the region of Southeast Europe, the
Griffon vulture is now confined to small and isolated populations across the Balkan
Peninsula. The population from Serbia represents its biggest and most viable
population that can serve as an important reservoir of genetic diversity from which
the birds can be used for the region’s reintroduction programmes. The available
genetic data for this valuable population are scarce and as a protected species that
belongs to the highly endangered vulture group, it needs to be well described so that it
can be properly managed and used as a restocking population. Considering the
serious recent bottleneck event that the Griffon vulture population from Serbia
experienced we estimated the overall relatedness among the birds from this
population. Sex ratio, another important parameter that shows the vitality and
strength of the population was evaluated as well.
Methods: During the annual monitoring that was performed in the period from
2013–2021, we collected blood samples from individual birds that were marked in the
nests. In total, 169 samples were collected and each was used for molecular sexing
while 58 presumably unrelated birds from different nests were used for inbreeding
and relatedness analyses. The relatedness was estimated using both biparentally (10
microsatellite loci) and uniparentally (Cytb and D-loop I of mitochondrial DNA)
inherited markers.
Results: The level of inbreeding was relatively high and on average it was 8.3% while
the mean number of relatives for each bird was close to three. The sex ratio was close
to 1:1 and for the analysed period of 9 years, it didn’t demonstrate a statistically
significant deviation from the expected ratio of 1:1, suggesting that this is a stable and
healthy population. Our data suggest that, even though a relatively high level of
inbreeding can be detected among the individual birds, the Griffon vulture
population from Serbia can be used as a source population for restocking and
reintroduction programmes in the region. These data combined with previously
observed genetic differentiation between the populations from the Iberian and
Balkan Peninsulas suggest that the introduction of foreign birds should be avoided
and that local birds should be used instead.",
publisher = "PeerJ",
journal = "PeerJ",
title = "Sex ratio and relatedness in the Griffon vulture (Gyps fulvus) population of Serbia",
volume = "10",
doi = "10.7717/peerj.14477",
pages = "e14477"
}

Davidović, S., Marinković, S., Hribšek, I., Patenković, A., Stamenković-Radak, M.,& Tanasković, M.. (2022). Sex ratio and relatedness in the Griffon vulture (Gyps fulvus) population of Serbia. in PeerJ
PeerJ., 10, e14477.
https://doi.org/10.7717/peerj.14477

Davidović S, Marinković S, Hribšek I, Patenković A, Stamenković-Radak M, Tanasković M. Sex ratio and relatedness in the Griffon vulture (Gyps fulvus) population of Serbia. in PeerJ. 2022;10:e14477.
doi:10.7717/peerj.14477 .

Davidović, Slobodan, Marinković, Saša, Hribšek, Irena, Patenković, Aleksandra, Stamenković-Radak, Marina, Tanasković, Marija, "Sex ratio and relatedness in the Griffon vulture (Gyps fulvus) population of Serbia" in PeerJ, 10 (2022):e14477,
https://doi.org/10.7717/peerj.14477 . .

TY  - CONF
AU  - Tanasković, Marija
AU  - Marinković, Saša
AU  - Hribšek, Irena
AU  - Patenković, Aleksandra
AU  - Anđus, Stefan
AU  - Stamenković-Radak, Marina
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5040
AB  - Beloglavi sup, Gyps fulvus, nekada široko rasprostranjena vrsta širom Jugoistočne Evrope danas je ograničen na male i izolovane populacije od kojih je na Balkanu najbrojnija na teritoriji Srbije. Genetička varijabilnost populacije beloglavog supa u Srbiji procenjena je na osnovu 58 ptica, na nivou mitohondrijske DNK analizom sekvence CytB i na nivou jedarne DNK analizom 10 mikrosatelitskih lokusa, dok je molekularnim markerima određen pol izleglih jedinki u periodu od 2013-2022 godine. Bez obzira na skorašnji drastičan pad brojnosti, nivo genetičke varijabilnosti procenjen na osnovu izabranih markera sličan je ostalim analiziranim populacijama u svetu. Analiza mikrosatelitskih lokusa je pokazala da populacija beloglavog supa iz Srbije poseduje najveći broj privatnih haplotipova, ali i nešto veći nivo inbridinga (8,3%). Detektovana su tri CytB haplotipa (jedan koji je prisutan u svim populacijama i dva karakteristična samo za populaciju iz Srbije). Na osnovu svih analiziranih markera, možemo reći da populacija supova u Srbiji poseduje genetičke osobenosti koje je jasno odvajaju od svih ostalih do sada analiziranih populacija kao i da je pokazan jednak odnos polova dodatni indikator stabilnosti i dobrog „zdravlja“ ove populacije. Populaciono genetičke analize pokazuju da je populacija beloglavog supa sa teritorije Srbije optimalni izbor za buduće programe reintrodukcije u regionu, uz napomenu da bi trebalo izbegavati reintrodukciju jedinki poreklom iz drugih geografskih područja.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Genetička varijabilnost populacije beloglavog supa na teritoriji Srbije – perspektiva za buduće programe reintrodukcije u regionu
SP  - 89
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5040
ER  - 

@conference{
author = "Tanasković, Marija and Marinković, Saša and Hribšek, Irena and Patenković, Aleksandra and Anđus, Stefan and Stamenković-Radak, Marina and Davidović, Slobodan",
year = "2022",
abstract = "Beloglavi sup, Gyps fulvus, nekada široko rasprostranjena vrsta širom Jugoistočne Evrope danas je ograničen na male i izolovane populacije od kojih je na Balkanu najbrojnija na teritoriji Srbije. Genetička varijabilnost populacije beloglavog supa u Srbiji procenjena je na osnovu 58 ptica, na nivou mitohondrijske DNK analizom sekvence CytB i na nivou jedarne DNK analizom 10 mikrosatelitskih lokusa, dok je molekularnim markerima određen pol izleglih jedinki u periodu od 2013-2022 godine. Bez obzira na skorašnji drastičan pad brojnosti, nivo genetičke varijabilnosti procenjen na osnovu izabranih markera sličan je ostalim analiziranim populacijama u svetu. Analiza mikrosatelitskih lokusa je pokazala da populacija beloglavog supa iz Srbije poseduje najveći broj privatnih haplotipova, ali i nešto veći nivo inbridinga (8,3%). Detektovana su tri CytB haplotipa (jedan koji je prisutan u svim populacijama i dva karakteristična samo za populaciju iz Srbije). Na osnovu svih analiziranih markera, možemo reći da populacija supova u Srbiji poseduje genetičke osobenosti koje je jasno odvajaju od svih ostalih do sada analiziranih populacija kao i da je pokazan jednak odnos polova dodatni indikator stabilnosti i dobrog „zdravlja“ ove populacije. Populaciono genetičke analize pokazuju da je populacija beloglavog supa sa teritorije Srbije optimalni izbor za buduće programe reintrodukcije u regionu, uz napomenu da bi trebalo izbegavati reintrodukciju jedinki poreklom iz drugih geografskih područja.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Genetička varijabilnost populacije beloglavog supa na teritoriji Srbije – perspektiva za buduće programe reintrodukcije u regionu",
pages = "89",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5040"
}

Tanasković, M., Marinković, S., Hribšek, I., Patenković, A., Anđus, S., Stamenković-Radak, M.,& Davidović, S.. (2022). Genetička varijabilnost populacije beloglavog supa na teritoriji Srbije – perspektiva za buduće programe reintrodukcije u regionu. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 89.
https://hdl.handle.net/21.15107/rcub_ibiss_5040

Tanasković M, Marinković S, Hribšek I, Patenković A, Anđus S, Stamenković-Radak M, Davidović S. Genetička varijabilnost populacije beloglavog supa na teritoriji Srbije – perspektiva za buduće programe reintrodukcije u regionu. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:89.
https://hdl.handle.net/21.15107/rcub_ibiss_5040 .

Tanasković, Marija, Marinković, Saša, Hribšek, Irena, Patenković, Aleksandra, Anđus, Stefan, Stamenković-Radak, Marina, Davidović, Slobodan, "Genetička varijabilnost populacije beloglavog supa na teritoriji Srbije – perspektiva za buduće programe reintrodukcije u regionu" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):89,
https://hdl.handle.net/21.15107/rcub_ibiss_5040 .

TY  - JOUR
AU  - Holub, Karolina
AU  - Malyarchuk, Boris
AU  - Derenko, Miroslava
AU  - Kovačević-Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
AU  - Davidović, Slobodan
AU  - Grzybowski, Tomasz
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5555
AB  - Genetic markers for the prediction of biogeographical ancestry have proved to be effective tools for law enforcement agencies for many years now. In this study, we attempted to assess the potential of insertion-deletion markers (InDel) and microsatellites (STRs) as subsidiary polymorphisms for inference of Slavic population ancestry. For that purpose, we genotyped Slavic-speaking populations samples from Belarus, the Czech Republic, Poland, Serbia, Ukraine and Russia in 46 InDels and 15 STRs by PCR and capillary electrophoresis and analyzed for between-population differentiation with the use of distance-based methods (FST, principal component analysis and multidimensional scaling).
Additionally, we studied a sample from a Polish individual of well-documented genealogy whose biogeographic ancestry had previously been inferred by commercial genomic services using autosomal single nucleotide polymorphisms (SNPs), mitochondrial DNA and Y-SNP markers. For comparative purposes, we used genotype data collected in the “forInDel” browser and allele frequencies from previously published papers. The results obtained for InDels and STRs show that the Slavic populations constitute a genetically homogeneous group, with the exception of the Czechs differing clearly from the other tested populations. The analysis of the known Polish sample in the Snipper application proves the usefulness of the InDel markers on the continental level only. Conversely, microsatellites not only improve prediction, but are also informative if considered as an independent set of ancestry markers.
PB  - Polish Society of Forensic Medicine and Criminology
T2  - Archives of Forensic Medicine and Criminology
T1  - Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry
IS  - 3
VL  - 72
DO  - 10.4467/16891716AMSIK.22.015.17393
SP  - 120
EP  - 137
ER  - 

@article{
author = "Holub, Karolina and Malyarchuk, Boris and Derenko, Miroslava and Kovačević-Grujičić, Nataša and Stevanović, Milena and Drakulić, Danijela and Davidović, Slobodan and Grzybowski, Tomasz",
year = "2022",
abstract = "Genetic markers for the prediction of biogeographical ancestry have proved to be effective tools for law enforcement agencies for many years now. In this study, we attempted to assess the potential of insertion-deletion markers (InDel) and microsatellites (STRs) as subsidiary polymorphisms for inference of Slavic population ancestry. For that purpose, we genotyped Slavic-speaking populations samples from Belarus, the Czech Republic, Poland, Serbia, Ukraine and Russia in 46 InDels and 15 STRs by PCR and capillary electrophoresis and analyzed for between-population differentiation with the use of distance-based methods (FST, principal component analysis and multidimensional scaling).
Additionally, we studied a sample from a Polish individual of well-documented genealogy whose biogeographic ancestry had previously been inferred by commercial genomic services using autosomal single nucleotide polymorphisms (SNPs), mitochondrial DNA and Y-SNP markers. For comparative purposes, we used genotype data collected in the “forInDel” browser and allele frequencies from previously published papers. The results obtained for InDels and STRs show that the Slavic populations constitute a genetically homogeneous group, with the exception of the Czechs differing clearly from the other tested populations. The analysis of the known Polish sample in the Snipper application proves the usefulness of the InDel markers on the continental level only. Conversely, microsatellites not only improve prediction, but are also informative if considered as an independent set of ancestry markers.",
publisher = "Polish Society of Forensic Medicine and Criminology",
journal = "Archives of Forensic Medicine and Criminology",
title = "Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry",
number = "3",
volume = "72",
doi = "10.4467/16891716AMSIK.22.015.17393",
pages = "120-137"
}

Holub, K., Malyarchuk, B., Derenko, M., Kovačević-Grujičić, N., Stevanović, M., Drakulić, D., Davidović, S.,& Grzybowski, T.. (2022). Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry. in Archives of Forensic Medicine and Criminology
Polish Society of Forensic Medicine and Criminology., 72(3), 120-137.
https://doi.org/10.4467/16891716AMSIK.22.015.17393

Holub K, Malyarchuk B, Derenko M, Kovačević-Grujičić N, Stevanović M, Drakulić D, Davidović S, Grzybowski T. Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry. in Archives of Forensic Medicine and Criminology. 2022;72(3):120-137.
doi:10.4467/16891716AMSIK.22.015.17393 .

Holub, Karolina, Malyarchuk, Boris, Derenko, Miroslava, Kovačević-Grujičić, Nataša, Stevanović, Milena, Drakulić, Danijela, Davidović, Slobodan, Grzybowski, Tomasz, "Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry" in Archives of Forensic Medicine and Criminology, 72, no. 3 (2022):120-137,
https://doi.org/10.4467/16891716AMSIK.22.015.17393 . .

TY  - JOUR
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Mihajlović, Milica
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - https://www.nature.com/articles/s41598-022-21413-y
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5084
AB  - Urbanization can change biodiversity in both directions, positive and negative, and despite the rising global trend of urban beekeeping, little is known about the impact of urbanization on the genetic diversity of honey bees. We investigate how urbanization affects the genetic variability of feral and managed honey bee colonies that are spread throughout the entire city, even in highly urban areas, through genetic analysis of 82 worker bees. We found convincing evidence of high genetic differentiation between these two groups. Additionally, by comparing city samples with 241 samples from 46 apiaries in rural parts of the country, variations in mitochondrial tRNAleu-cox2 intergenic region and microsatellite loci indicated that feral colonies have distinct patterns of genetic diversity. These results, with evidence that feral honey bees find niches within highly modified and human-dominated urban landscapes, lead us to conclude that urbanization is a driver of the genetic diversity of feral honey bees in the city.
PB  - Berlin: Nature Portfolio
T2  - Scientific Reports
T1  - Urban ecosystem drives genetic diversity in feral honey bee.
IS  - 1
VL  - 12
DO  - 10.1038/s41598-022-21413-y
SP  - 17692
ER  - 

@article{
author = "Patenković, Aleksandra and Tanasković, Marija and Erić, Pavle and Erić, Katarina and Mihajlović, Milica and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Urbanization can change biodiversity in both directions, positive and negative, and despite the rising global trend of urban beekeeping, little is known about the impact of urbanization on the genetic diversity of honey bees. We investigate how urbanization affects the genetic variability of feral and managed honey bee colonies that are spread throughout the entire city, even in highly urban areas, through genetic analysis of 82 worker bees. We found convincing evidence of high genetic differentiation between these two groups. Additionally, by comparing city samples with 241 samples from 46 apiaries in rural parts of the country, variations in mitochondrial tRNAleu-cox2 intergenic region and microsatellite loci indicated that feral colonies have distinct patterns of genetic diversity. These results, with evidence that feral honey bees find niches within highly modified and human-dominated urban landscapes, lead us to conclude that urbanization is a driver of the genetic diversity of feral honey bees in the city.",
publisher = "Berlin: Nature Portfolio",
journal = "Scientific Reports",
title = "Urban ecosystem drives genetic diversity in feral honey bee.",
number = "1",
volume = "12",
doi = "10.1038/s41598-022-21413-y",
pages = "17692"
}

Patenković, A., Tanasković, M., Erić, P., Erić, K., Mihajlović, M., Stanisavljević, L.,& Davidović, S.. (2022). Urban ecosystem drives genetic diversity in feral honey bee.. in Scientific Reports
Berlin: Nature Portfolio., 12(1), 17692.
https://doi.org/10.1038/s41598-022-21413-y

Patenković A, Tanasković M, Erić P, Erić K, Mihajlović M, Stanisavljević L, Davidović S. Urban ecosystem drives genetic diversity in feral honey bee.. in Scientific Reports. 2022;12(1):17692.
doi:10.1038/s41598-022-21413-y .

Patenković, Aleksandra, Tanasković, Marija, Erić, Pavle, Erić, Katarina, Mihajlović, Milica, Stanisavljević, Ljubiša, Davidović, Slobodan, "Urban ecosystem drives genetic diversity in feral honey bee." in Scientific Reports, 12, no. 1 (2022):17692,
https://doi.org/10.1038/s41598-022-21413-y . .

TY  - JOUR
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Tanasković, Marija
AU  - Davidović, Slobodan
AU  - Rakić, Mina
AU  - Savić Veselinović, Marija
AU  - Stamenković-Radak, Marina
AU  - Jelić, Mihailo
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4892
AB  - The adaptive significance of sympatric mitochondrial (mtDNA) variation and the role of
selective mechanisms that maintain it are debated to this day. Isofemale lines of Drosophila obscura
collected from four populations were backcrossed within populations to construct experimental
lines, with all combinations of mtDNA Cyt b haplotypes and nuclear genetic backgrounds (nuDNA).
Individuals of both sexes from these lines were then subjected to four fitness assays (desiccation
resistance, developmental time, egg-to-adult viability and sex ratio) on two experimental temperatures to examine the role of temperature fluctuations and sex-specific selection, as well as the part
that interactions between the two genomes play in shaping mtDNA variation. The results varied
across populations and fitness components. In the majority of comparisons, they show that sympatric
mitochondrial variants affect fitness. However, their effect should be examined in light of interactions
with nuDNA, as mito-nuclear genotype was even more influential on fitness across all components.
We found both sex-specific and temperature-specific differences in mitochondrial and mito-nuclear
genotype ranks in all fitness components. The effect of temperature-specific selection was found to
be more prominent, especially in desiccation resistance. From the results of different components
tested, we can also infer that temperature-specific mito-nuclear interactions rather than sex-specific
selection on mito-nuclear genotypes have a more substantial role in preserving mtDNA variation in
this model species.
PB  - Basel: MDPI
T2  - Insects
T1  - Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura
IS  - 2
VL  - 13
DO  - 10.3390/insects13020139
SP  - 139
ER  - 

@article{
author = "Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Tanasković, Marija and Davidović, Slobodan and Rakić, Mina and Savić Veselinović, Marija and Stamenković-Radak, Marina and Jelić, Mihailo",
year = "2022",
abstract = "The adaptive significance of sympatric mitochondrial (mtDNA) variation and the role of
selective mechanisms that maintain it are debated to this day. Isofemale lines of Drosophila obscura
collected from four populations were backcrossed within populations to construct experimental
lines, with all combinations of mtDNA Cyt b haplotypes and nuclear genetic backgrounds (nuDNA).
Individuals of both sexes from these lines were then subjected to four fitness assays (desiccation
resistance, developmental time, egg-to-adult viability and sex ratio) on two experimental temperatures to examine the role of temperature fluctuations and sex-specific selection, as well as the part
that interactions between the two genomes play in shaping mtDNA variation. The results varied
across populations and fitness components. In the majority of comparisons, they show that sympatric
mitochondrial variants affect fitness. However, their effect should be examined in light of interactions
with nuDNA, as mito-nuclear genotype was even more influential on fitness across all components.
We found both sex-specific and temperature-specific differences in mitochondrial and mito-nuclear
genotype ranks in all fitness components. The effect of temperature-specific selection was found to
be more prominent, especially in desiccation resistance. From the results of different components
tested, we can also infer that temperature-specific mito-nuclear interactions rather than sex-specific
selection on mito-nuclear genotypes have a more substantial role in preserving mtDNA variation in
this model species.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura",
number = "2",
volume = "13",
doi = "10.3390/insects13020139",
pages = "139"
}

Erić, P., Patenković, A., Erić, K., Tanasković, M., Davidović, S., Rakić, M., Savić Veselinović, M., Stamenković-Radak, M.,& Jelić, M.. (2022). Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura. in Insects
Basel: MDPI., 13(2), 139.
https://doi.org/10.3390/insects13020139

Erić P, Patenković A, Erić K, Tanasković M, Davidović S, Rakić M, Savić Veselinović M, Stamenković-Radak M, Jelić M. Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura. in Insects. 2022;13(2):139.
doi:10.3390/insects13020139 .

Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Tanasković, Marija, Davidović, Slobodan, Rakić, Mina, Savić Veselinović, Marija, Stamenković-Radak, Marina, Jelić, Mihailo, "Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura" in Insects, 13, no. 2 (2022):139,
https://doi.org/10.3390/insects13020139 . .

TY  - JOUR
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Kusza, Szilvia
AU  - Oleksa, Andrzej
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4806
AB  - Socioeconomic interests and beekeeper preferences have often taken precedence over the conservation of locally native honey bee subspecies, leading to the predominance of admixture populations in human-dominated areas. To assess the genetic diversity of contemporary managed Serbian honey bee colonies, we used 14 microsatellite loci and analyzed 237 worker bees from 46 apiaries in eight localities of northern and southern Serbia. Furthermore, we compared data for nine microsatellite loci with 338 individuals from Italy, Hungary, Poland, and Spain. The standard parameters of genetic diversity in Serbian honey bee populations were in line with other analyses, although somewhat smaller. STRUCTURE analysis showed the existence of two equally distributed genetic clusters and Analysis of molecular variances could not confirm the presence of a geographically discrete population but showed local differences. Discriminant analysis of principal components showed overlapping of worker bees from different parts of Serbia. Clear genetic differentiation can be observed when comparing all populations between geographical regions and their corresponding subspecies. The absence of the A. m. macedonica subspecies from its historical distribution range in southern Serbia as well as the lack of distinctive geographical groups suggest that selective breeding, queen import, and migratory beekeeping practices strongly influence the genetic structure and diversity of honey bees, leading to the genetic uniformization and creation of the admixture population.
PB  - Basel: MDPI
T2  - Insects
T1  - Further Evidence of Population Admixture in the Serbian Honey Bee Population
IS  - 2
VL  - 13
DO  - 10.3390/insects13020180
SP  - 180
ER  - 

@article{
author = "Tanasković, Marija and Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Mihajlović, Milica and Tanasić, Vanja and Kusza, Szilvia and Oleksa, Andrzej and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Socioeconomic interests and beekeeper preferences have often taken precedence over the conservation of locally native honey bee subspecies, leading to the predominance of admixture populations in human-dominated areas. To assess the genetic diversity of contemporary managed Serbian honey bee colonies, we used 14 microsatellite loci and analyzed 237 worker bees from 46 apiaries in eight localities of northern and southern Serbia. Furthermore, we compared data for nine microsatellite loci with 338 individuals from Italy, Hungary, Poland, and Spain. The standard parameters of genetic diversity in Serbian honey bee populations were in line with other analyses, although somewhat smaller. STRUCTURE analysis showed the existence of two equally distributed genetic clusters and Analysis of molecular variances could not confirm the presence of a geographically discrete population but showed local differences. Discriminant analysis of principal components showed overlapping of worker bees from different parts of Serbia. Clear genetic differentiation can be observed when comparing all populations between geographical regions and their corresponding subspecies. The absence of the A. m. macedonica subspecies from its historical distribution range in southern Serbia as well as the lack of distinctive geographical groups suggest that selective breeding, queen import, and migratory beekeeping practices strongly influence the genetic structure and diversity of honey bees, leading to the genetic uniformization and creation of the admixture population.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "Further Evidence of Population Admixture in the Serbian Honey Bee Population",
number = "2",
volume = "13",
doi = "10.3390/insects13020180",
pages = "180"
}

Tanasković, M., Erić, P., Patenković, A., Erić, K., Mihajlović, M., Tanasić, V., Kusza, S., Oleksa, A., Stanisavljević, L.,& Davidović, S.. (2022). Further Evidence of Population Admixture in the Serbian Honey Bee Population. in Insects
Basel: MDPI., 13(2), 180.
https://doi.org/10.3390/insects13020180

Tanasković M, Erić P, Patenković A, Erić K, Mihajlović M, Tanasić V, Kusza S, Oleksa A, Stanisavljević L, Davidović S. Further Evidence of Population Admixture in the Serbian Honey Bee Population. in Insects. 2022;13(2):180.
doi:10.3390/insects13020180 .

Tanasković, Marija, Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Mihajlović, Milica, Tanasić, Vanja, Kusza, Szilvia, Oleksa, Andrzej, Stanisavljević, Ljubiša, Davidović, Slobodan, "Further Evidence of Population Admixture in the Serbian Honey Bee Population" in Insects, 13, no. 2 (2022):180,
https://doi.org/10.3390/insects13020180 . .

TY  - JOUR
AU  - Erić, Katarina
AU  - Patenković, Aleksandra
AU  - Erić, Pavle
AU  - Davidović, Slobodan
AU  - Savić Veselinović, Marija
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
PY  - 2022
UR  - https://www.mdpi.com/2075-4450/13/2/138
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4800
AB  - Global warming and climate change are affecting many insect species in numerous ways. These species can develop diverse mechanisms as a response to variable environmental conditions. The rise in mean and extreme temperatures due to global warming and the importance of the population’s ability to adapt to temperature stress will further increase. In this study, we investigated thermal stress response, which is considered to be one of the crucial elements of population fitness and survival in fast-changing environments. The dynamics and variation of thermal stress resistance traits in D. subobscura flies originating from two natural populations sampled from different altitudes were analysed. Three different temperature regimes (25 °C, 19 °C, and 16 °C) were used for the F1 progeny from both localities to establish six experimental groups and investigate stress resistance traits: desiccation resistance, heat knock-down resistance, starvation resistance, and chill-coma recovery time. We detected that laboratory thermal conditions and population origin may have an effect on the analysed traits, and that sex also significantly influences stress resistance. Individuals from the lower altitude reared at higher temperatures show inferior resistance to thermal shock.
PB  - Basel: MDPI
T2  - Insects
T1  - Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes
IS  - 2
VL  - 13
DO  - 10.3390/insects13020138
SP  - 138
ER  - 

@article{
author = "Erić, Katarina and Patenković, Aleksandra and Erić, Pavle and Davidović, Slobodan and Savić Veselinović, Marija and Stamenković-Radak, Marina and Tanasković, Marija",
year = "2022",
abstract = "Global warming and climate change are affecting many insect species in numerous ways. These species can develop diverse mechanisms as a response to variable environmental conditions. The rise in mean and extreme temperatures due to global warming and the importance of the population’s ability to adapt to temperature stress will further increase. In this study, we investigated thermal stress response, which is considered to be one of the crucial elements of population fitness and survival in fast-changing environments. The dynamics and variation of thermal stress resistance traits in D. subobscura flies originating from two natural populations sampled from different altitudes were analysed. Three different temperature regimes (25 °C, 19 °C, and 16 °C) were used for the F1 progeny from both localities to establish six experimental groups and investigate stress resistance traits: desiccation resistance, heat knock-down resistance, starvation resistance, and chill-coma recovery time. We detected that laboratory thermal conditions and population origin may have an effect on the analysed traits, and that sex also significantly influences stress resistance. Individuals from the lower altitude reared at higher temperatures show inferior resistance to thermal shock.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes",
number = "2",
volume = "13",
doi = "10.3390/insects13020138",
pages = "138"
}

Erić, K., Patenković, A., Erić, P., Davidović, S., Savić Veselinović, M., Stamenković-Radak, M.,& Tanasković, M.. (2022). Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes. in Insects
Basel: MDPI., 13(2), 138.
https://doi.org/10.3390/insects13020138

Erić K, Patenković A, Erić P, Davidović S, Savić Veselinović M, Stamenković-Radak M, Tanasković M. Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes. in Insects. 2022;13(2):138.
doi:10.3390/insects13020138 .

Erić, Katarina, Patenković, Aleksandra, Erić, Pavle, Davidović, Slobodan, Savić Veselinović, Marija, Stamenković-Radak, Marina, Tanasković, Marija, "Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes" in Insects, 13, no. 2 (2022):138,
https://doi.org/10.3390/insects13020138 . .

TY  - CONF
AU  - Stanisavljević, Ljubiša
AU  - Bila-Dubaić, Jovana
AU  - SImonović, Slađan
AU  - Plećaš, Milan
AU  - Davidović, Slobodan
AU  - Tanasković, Marija
AU  - Ćetković, Aleksandar
PY  - 2022
UR  - http://www.parconazionalepantelleria.it/international_meeting_apis_silvatica.php
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5965
AB  - In the modern world managed bee species are necessary for the stability and sustainability of food production, of which the western honey bee (Apis mellifera) is the best known and the single most important species (Kevan, 2007). Wild honey bees have largely become extinct throughout Europe since the 1980s, following the introduction of the ectoparasitic mite Varroa destructor and various pathogens associated with it (Crane, 1999). The honey bee has been the subject of extensive research around the world for decades, particularly in terms of preserving the health and stability of their colonies under these new pressures. In that period, the importance of rare, accidentally surviving wild and feral colonies of honey bees was largely neglected. However, several recent studies (e.g., Le Conte et al., 2007) report on the growing number of colonies that have successfully survived the Varroa mite infestation without usual chemical treatments.
Unlike in other studies on unmanaged colonies, we presented a special case of the free-living population of honey bee in a large and highly populated urban environment of Belgrade, the capital of Serbia (Southeast Europe). During the period 2011–2017, we collected a large set of observational data (>1,300 records) from the apparently dense wild/feral honey bee population. Large share of these records can not be spatially related to the existence of managed apiaries, which are very rare in the core of downtown Belgrade. We hypothesized that numerous unmanaged bee colonies and the high frequency of swarms indicate a stable, self-sustaining wild population, and not the regular influx of swarms from the managed apiaries situated more peripherally. Also, we elaborated on various plausible explanations for this extraordinary finding.
By geospatial analysis of different categories of occurrence (colonies and swarms observed in different habitats and micro-situations) in relation to the parameters of urban environments suitability for honey bees, we evaluated the patterns of distribution and long-term survival of feral colonies under urban conditions. The results indicate an extremely high and stable population density and unusually long persistence of feral colonies, which is a rare phenomenon on a global scale (Bila Dubaić et al., 2021). Previous isolated cases of unmanaged colony survival (unknown in urban conditions) triggered great scientific attention: understanding the factors behind these survival cases may contribute to the efforts on improving the managed honey bee health. Therefore, our results contribute to ongoing initiatives to support naturally selected resistance mechanisms against Varroa mites, and hopefully reduce the currently growing incidence of colony die-offs.
The collecting of data was based on opportunistic citizen science, which we hereby evaluate as a highly prospective approach in the study of wild/feral bees in urban areas. We believe that specifically designed citizen science projects, based on our experiences, could be implemented with a great success in other countries with similar highly urbanised circumstances; in particular, investigations should be focused on environments with comparable potentials for the unnoticed existence of unmanaged honey bees (Bila Dubaić et al., 2021).
Despite the growing global trend of urban beekeeping, little is known about the impact of urbanization on the genetic diversity of honey bees. In particular, we investigated and performed genetic analysis of 82 individual bee genomes in a portion of a sample of feral and managed honey bee colonies distributed throughout this large city, including highly urbanised areas. We established the existence of a high genetic differentiation between these two groups. Also, the comparison of mitochondrial and microsatellite DNA of bees sampled in Belgrade and in rural parts of Serbia showed that colonies of wild/feral bees have different patterns of genetic diversity. It can be concluded that urbanisation can be a positive driver of the genetic diversity of wild honey bees nesting in a highly urbanised and densely populated areas.
PB  - Isola di Pantelleria: Ente Parco Nazionale
C3  - First international meeting APIS SILVATICA: The western honey bee in nature; 2022 May 16-20; Pantelleria, Italy
T1  - Extraordinary incidence and persistence of feral honey bees in urban environments of Belgrade (Serbia)
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5965
ER  - 

@conference{
author = "Stanisavljević, Ljubiša and Bila-Dubaić, Jovana and SImonović, Slađan and Plećaš, Milan and Davidović, Slobodan and Tanasković, Marija and Ćetković, Aleksandar",
year = "2022",
abstract = "In the modern world managed bee species are necessary for the stability and sustainability of food production, of which the western honey bee (Apis mellifera) is the best known and the single most important species (Kevan, 2007). Wild honey bees have largely become extinct throughout Europe since the 1980s, following the introduction of the ectoparasitic mite Varroa destructor and various pathogens associated with it (Crane, 1999). The honey bee has been the subject of extensive research around the world for decades, particularly in terms of preserving the health and stability of their colonies under these new pressures. In that period, the importance of rare, accidentally surviving wild and feral colonies of honey bees was largely neglected. However, several recent studies (e.g., Le Conte et al., 2007) report on the growing number of colonies that have successfully survived the Varroa mite infestation without usual chemical treatments.
Unlike in other studies on unmanaged colonies, we presented a special case of the free-living population of honey bee in a large and highly populated urban environment of Belgrade, the capital of Serbia (Southeast Europe). During the period 2011–2017, we collected a large set of observational data (>1,300 records) from the apparently dense wild/feral honey bee population. Large share of these records can not be spatially related to the existence of managed apiaries, which are very rare in the core of downtown Belgrade. We hypothesized that numerous unmanaged bee colonies and the high frequency of swarms indicate a stable, self-sustaining wild population, and not the regular influx of swarms from the managed apiaries situated more peripherally. Also, we elaborated on various plausible explanations for this extraordinary finding.
By geospatial analysis of different categories of occurrence (colonies and swarms observed in different habitats and micro-situations) in relation to the parameters of urban environments suitability for honey bees, we evaluated the patterns of distribution and long-term survival of feral colonies under urban conditions. The results indicate an extremely high and stable population density and unusually long persistence of feral colonies, which is a rare phenomenon on a global scale (Bila Dubaić et al., 2021). Previous isolated cases of unmanaged colony survival (unknown in urban conditions) triggered great scientific attention: understanding the factors behind these survival cases may contribute to the efforts on improving the managed honey bee health. Therefore, our results contribute to ongoing initiatives to support naturally selected resistance mechanisms against Varroa mites, and hopefully reduce the currently growing incidence of colony die-offs.
The collecting of data was based on opportunistic citizen science, which we hereby evaluate as a highly prospective approach in the study of wild/feral bees in urban areas. We believe that specifically designed citizen science projects, based on our experiences, could be implemented with a great success in other countries with similar highly urbanised circumstances; in particular, investigations should be focused on environments with comparable potentials for the unnoticed existence of unmanaged honey bees (Bila Dubaić et al., 2021).
Despite the growing global trend of urban beekeeping, little is known about the impact of urbanization on the genetic diversity of honey bees. In particular, we investigated and performed genetic analysis of 82 individual bee genomes in a portion of a sample of feral and managed honey bee colonies distributed throughout this large city, including highly urbanised areas. We established the existence of a high genetic differentiation between these two groups. Also, the comparison of mitochondrial and microsatellite DNA of bees sampled in Belgrade and in rural parts of Serbia showed that colonies of wild/feral bees have different patterns of genetic diversity. It can be concluded that urbanisation can be a positive driver of the genetic diversity of wild honey bees nesting in a highly urbanised and densely populated areas.",
publisher = "Isola di Pantelleria: Ente Parco Nazionale",
journal = "First international meeting APIS SILVATICA: The western honey bee in nature; 2022 May 16-20; Pantelleria, Italy",
title = "Extraordinary incidence and persistence of feral honey bees in urban environments of Belgrade (Serbia)",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5965"
}

Stanisavljević, L., Bila-Dubaić, J., SImonović, S., Plećaš, M., Davidović, S., Tanasković, M.,& Ćetković, A.. (2022). Extraordinary incidence and persistence of feral honey bees in urban environments of Belgrade (Serbia). in First international meeting APIS SILVATICA: The western honey bee in nature; 2022 May 16-20; Pantelleria, Italy
Isola di Pantelleria: Ente Parco Nazionale..
https://hdl.handle.net/21.15107/rcub_ibiss_5965

Stanisavljević L, Bila-Dubaić J, SImonović S, Plećaš M, Davidović S, Tanasković M, Ćetković A. Extraordinary incidence and persistence of feral honey bees in urban environments of Belgrade (Serbia). in First international meeting APIS SILVATICA: The western honey bee in nature; 2022 May 16-20; Pantelleria, Italy. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5965 .

Stanisavljević, Ljubiša, Bila-Dubaić, Jovana, SImonović, Slađan, Plećaš, Milan, Davidović, Slobodan, Tanasković, Marija, Ćetković, Aleksandar, "Extraordinary incidence and persistence of feral honey bees in urban environments of Belgrade (Serbia)" in First international meeting APIS SILVATICA: The western honey bee in nature; 2022 May 16-20; Pantelleria, Italy (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5965 .

TY  - JOUR
AU  - Bila Dubaić, Jovana
AU  - Dall’Olio, Raffaele
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5379
AB  - Background
In the past years, a surprisingly large number of free-living honey bee colonies was recorded in Belgrade, capital of Serbia (Bila Dubaić et al., 2021). Since there are no public city services that citizens could turn to if they notice a colony of free-living honey bees in their proximity, a large portion of such cases are reported to beekeepers. Owing mostly to the engagement of one Belgrade beekeeper, scientists of the Faculty of Biology University of Belgrade obtained a large set of data on free-living honey bees in Belgrade. The collected data covers a period of 7 years (2011–2017) and, after elimination of dubious and duplicated reports, the number of recorded swarms and colonies exceeded 1300.
PB  - Taylor and Francis
T2  - Bee World
T1  - Insight into Free-Living Honey Bee Population in Serbia’s Capital – A COLOSSal Walk around Belgrade
DO  - 10.1080/0005772X.2022.2151721
ER  - 

@article{
author = "Bila Dubaić, Jovana and Dall’Olio, Raffaele and Davidović, Slobodan",
year = "2022",
abstract = "Background
In the past years, a surprisingly large number of free-living honey bee colonies was recorded in Belgrade, capital of Serbia (Bila Dubaić et al., 2021). Since there are no public city services that citizens could turn to if they notice a colony of free-living honey bees in their proximity, a large portion of such cases are reported to beekeepers. Owing mostly to the engagement of one Belgrade beekeeper, scientists of the Faculty of Biology University of Belgrade obtained a large set of data on free-living honey bees in Belgrade. The collected data covers a period of 7 years (2011–2017) and, after elimination of dubious and duplicated reports, the number of recorded swarms and colonies exceeded 1300.",
publisher = "Taylor and Francis",
journal = "Bee World",
title = "Insight into Free-Living Honey Bee Population in Serbia’s Capital – A COLOSSal Walk around Belgrade",
doi = "10.1080/0005772X.2022.2151721"
}

Bila Dubaić, J., Dall’Olio, R.,& Davidović, S.. (2022). Insight into Free-Living Honey Bee Population in Serbia’s Capital – A COLOSSal Walk around Belgrade. in Bee World
Taylor and Francis..
https://doi.org/10.1080/0005772X.2022.2151721

Bila Dubaić J, Dall’Olio R, Davidović S. Insight into Free-Living Honey Bee Population in Serbia’s Capital – A COLOSSal Walk around Belgrade. in Bee World. 2022;.
doi:10.1080/0005772X.2022.2151721 .

Bila Dubaić, Jovana, Dall’Olio, Raffaele, Davidović, Slobodan, "Insight into Free-Living Honey Bee Population in Serbia’s Capital – A COLOSSal Walk around Belgrade" in Bee World (2022),
https://doi.org/10.1080/0005772X.2022.2151721 . .

TY  - JOUR
AU  - Bila Dubaić, Jovana
AU  - Dall’Olio, Raffaele
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5379
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5380
AB  - BackgroundIn the past years, a surprisingly large number of free-living honey bee colonies was recorded in Belgrade, capital of Serbia (Bila Dubaić et al., 2021). Since there are no public city services that citizens could turn to if they notice a colony of free-living honey bees in their proximity, a large portion of such cases are reported to beekeepers. Owing mostly to the engagement of one Belgrade beekeeper, scientists of the Faculty of Biology University of Belgrade obtained a large set of data on free-living honey bees in Belgrade. The collected data covers a period of 7 years (2011–2017) and, after elimination of dubious and duplicated reports, the number of recorded swarms and colonies exceeded 1300.
PB  - Taylor and Francis
T2  - Bee World
T1  - Insight into Free-Living Honey Bee Population in Serbia’s Capital – A COLOSSal Walk around Belgrade
DO  - 10.1080/0005772X.2022.2151721
ER  - 

@article{
author = "Bila Dubaić, Jovana and Dall’Olio, Raffaele and Davidović, Slobodan",
year = "2022",
abstract = "BackgroundIn the past years, a surprisingly large number of free-living honey bee colonies was recorded in Belgrade, capital of Serbia (Bila Dubaić et al., 2021). Since there are no public city services that citizens could turn to if they notice a colony of free-living honey bees in their proximity, a large portion of such cases are reported to beekeepers. Owing mostly to the engagement of one Belgrade beekeeper, scientists of the Faculty of Biology University of Belgrade obtained a large set of data on free-living honey bees in Belgrade. The collected data covers a period of 7 years (2011–2017) and, after elimination of dubious and duplicated reports, the number of recorded swarms and colonies exceeded 1300.",
publisher = "Taylor and Francis",
journal = "Bee World",
title = "Insight into Free-Living Honey Bee Population in Serbia’s Capital – A COLOSSal Walk around Belgrade",
doi = "10.1080/0005772X.2022.2151721"
}

Bila Dubaić, J., Dall’Olio, R.,& Davidović, S.. (2022). Insight into Free-Living Honey Bee Population in Serbia’s Capital – A COLOSSal Walk around Belgrade. in Bee World
Taylor and Francis..
https://doi.org/10.1080/0005772X.2022.2151721

Bila Dubaić J, Dall’Olio R, Davidović S. Insight into Free-Living Honey Bee Population in Serbia’s Capital – A COLOSSal Walk around Belgrade. in Bee World. 2022;.
doi:10.1080/0005772X.2022.2151721 .

Bila Dubaić, Jovana, Dall’Olio, Raffaele, Davidović, Slobodan, "Insight into Free-Living Honey Bee Population in Serbia’s Capital – A COLOSSal Walk around Belgrade" in Bee World (2022),
https://doi.org/10.1080/0005772X.2022.2151721 . .

TY  - DATA
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Mihailović, Milica
AU  - Tanasić, Vanja
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4954
AB  - Figure S5. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 14 microsatellite loci (A7, A8, A14, A24, A28, A35, A43, A79, A88, A107, A113, Ap43, Ap249 and B124) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica), South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia including the Belgrade and for the feral honey bees that were found on different locations in Belgrade. In figure S5 the first three principal components (PC) are presented.
T1  - Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee"
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4954
ER  - 

@misc{
author = "Patenković, Aleksandra and Tanasković, Marija and Erić, Pavle and Erić, Katarina and Mihailović, Milica and Tanasić, Vanja and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Figure S5. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 14 microsatellite loci (A7, A8, A14, A24, A28, A35, A43, A79, A88, A107, A113, Ap43, Ap249 and B124) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica), South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia including the Belgrade and for the feral honey bees that were found on different locations in Belgrade. In figure S5 the first three principal components (PC) are presented.",
title = "Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee"",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4954"
}

Patenković, A., Tanasković, M., Erić, P., Erić, K., Mihailović, M., Tanasić, V., Stanisavljević, L.,& Davidović, S.. (2022). Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee". .
https://hdl.handle.net/21.15107/rcub_ibiss_4954

Patenković A, Tanasković M, Erić P, Erić K, Mihailović M, Tanasić V, Stanisavljević L, Davidović S. Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee". 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_4954 .

Patenković, Aleksandra, Tanasković, Marija, Erić, Pavle, Erić, Katarina, Mihailović, Milica, Tanasić, Vanja, Stanisavljević, Ljubiša, Davidović, Slobodan, "Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee"" (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_4954 .

TY  - CONF
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5039
AB  - Medonosna pčela (Apis mellifera) u Srbiji je poslednjih decenija pod uticajem čoveka usled ekspanzije pčelarstva. Pored naglog povećanja broja pčelara, košnica i selekcionih centara, savremene pčelarske tehnike uključuju i trgovinu maticama, veštačku selekciju i migratorno pčelarstvo, što utiče i na genetičku raznovrsnost gajenih i feralnih populacija ove ekološki i ekonomski važne vrste. Uporedne analize nuklearnog i mitohondrijskog (mtDNK) genoma radilica iz feralnih društava (56) i pčelinjaka (542) širom Srbije, pokazale su da feralna društva imaju veće vrednosti parametara genetičke raznovrsnosti za oba genetička markera u poređenju sa gajenim pčelama. Procene srodnosti su pokazale da su beogradska feralna društva međusobno srodnija nego sa drugim gajenim društvima sa teritorije Beograda, što ukazuje na dobro uspostavljenu populaciju feralnih pčela u glavnom gradu. Rezultati analize mtDNK ukazuju na značajnu promenu u distribuciji i raznovrsnosti mtDNK linija gajenih pčela u poređenju sa podacima za Srbiju iz ranijih decenija, a rezultati analize polimorfnosti 14 mikrosatelitska lokusa - na gubitak genetičke raznovrsnosti i veliku uniformnost gajenih pčela. Ovo ukazuje na urgentnu potrebu za promenom pčelarske prakse i za očuvanjem kako lokalno adaptiranih populacija, tj. različitih ekotipova karakterističnih za određene regione Srbije, tako i feralnih populacija, čime bi se obezbedila zaštita genetičkog diverziteta i opstanak medonosne pčele u Srbiji.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Medonosne pčele Srbije – populaciono-genetički pogled
SP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5039
ER  - 

@conference{
author = "Patenković, Aleksandra and Tanasković, Marija and Erić, Pavle and Erić, Katarina and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Medonosna pčela (Apis mellifera) u Srbiji je poslednjih decenija pod uticajem čoveka usled ekspanzije pčelarstva. Pored naglog povećanja broja pčelara, košnica i selekcionih centara, savremene pčelarske tehnike uključuju i trgovinu maticama, veštačku selekciju i migratorno pčelarstvo, što utiče i na genetičku raznovrsnost gajenih i feralnih populacija ove ekološki i ekonomski važne vrste. Uporedne analize nuklearnog i mitohondrijskog (mtDNK) genoma radilica iz feralnih društava (56) i pčelinjaka (542) širom Srbije, pokazale su da feralna društva imaju veće vrednosti parametara genetičke raznovrsnosti za oba genetička markera u poređenju sa gajenim pčelama. Procene srodnosti su pokazale da su beogradska feralna društva međusobno srodnija nego sa drugim gajenim društvima sa teritorije Beograda, što ukazuje na dobro uspostavljenu populaciju feralnih pčela u glavnom gradu. Rezultati analize mtDNK ukazuju na značajnu promenu u distribuciji i raznovrsnosti mtDNK linija gajenih pčela u poređenju sa podacima za Srbiju iz ranijih decenija, a rezultati analize polimorfnosti 14 mikrosatelitska lokusa - na gubitak genetičke raznovrsnosti i veliku uniformnost gajenih pčela. Ovo ukazuje na urgentnu potrebu za promenom pčelarske prakse i za očuvanjem kako lokalno adaptiranih populacija, tj. različitih ekotipova karakterističnih za određene regione Srbije, tako i feralnih populacija, čime bi se obezbedila zaštita genetičkog diverziteta i opstanak medonosne pčele u Srbiji.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Medonosne pčele Srbije – populaciono-genetički pogled",
pages = "87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5039"
}

Patenković, A., Tanasković, M., Erić, P., Erić, K., Stanisavljević, L.,& Davidović, S.. (2022). Medonosne pčele Srbije – populaciono-genetički pogled. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 87.
https://hdl.handle.net/21.15107/rcub_ibiss_5039

Patenković A, Tanasković M, Erić P, Erić K, Stanisavljević L, Davidović S. Medonosne pčele Srbije – populaciono-genetički pogled. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:87.
https://hdl.handle.net/21.15107/rcub_ibiss_5039 .

Patenković, Aleksandra, Tanasković, Marija, Erić, Pavle, Erić, Katarina, Stanisavljević, Ljubiša, Davidović, Slobodan, "Medonosne pčele Srbije – populaciono-genetički pogled" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):87,
https://hdl.handle.net/21.15107/rcub_ibiss_5039 .

TY  - CONF
AU  - Erić, Pavle
AU  - Tanasković, Marija
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5037
AB  - Zapadna medonosna pčela je kosmopolit i jedan od najznačajnih oprašivača na planeti zemlji. Smatra se da je vrsta nastala na afričkom kontinentu odakle se prirodno proširila u Evropu, Bliski istok i zapadne delove Azije. Čovek je shvativši ekonomski značaj ove vrste medonosnu pčelu preneo u Severnu i Južnu Ameriku, istočnu Aziju, Australiju i Novi Zeland. Medonosna pčela se šireći svoj areal prilagoñavala novim životnim sredinama, sinhronizujući svoje aktivnosti sa ciklusima cvetanja lokalnih biljaka, kao i sa klimatskim faktorima u novim sredinama, pa tako imamo 33 opisane podvrste Apis mellifera. Ove podvrste podeljene su u 5 evolutivnih linija označenih sa A, M, C, O i Y, korišćenjem morfometrijskih kao i genetičkih markera. Linija A je karakteristična za Afrički kontinent, dok su M i C karakteristične za Severozapadnu i Jugoistočnu Evropu. Linije O i Y karakteristične su za Bliski istok.
U okviru programa za izvrsne projekte mladih istraživača (PROMIS), finansiranog od strane fonda za nauku Republike Srbije realizovan je projekat istraživanja genetičke raznovrsnosti gajenih i divljih društava medonosne pčele Apis mellifera (SERBHIWE). Tokom 2020 i 2021 godine, organizovan je terenski rad na kome su za genetička istraživanja uzorkovani pčelinjaci poreklom sa juga, severa, istoka i zapada Srbije. Uporedo su uzorkovani i pčelinjaci sa teritorije Beograda. Za vreme trajanja projekta uzorkovano je ukupno 102 pčelinjaka i 930 pčelinjih društava (košnica), od kojih je 542 odabrano za analizu genetičke raznovrsnosti mitohondrijske DNK.
Kako bi što bolje prezentovali genetičku raznovrsnost karakterističnu za lokalno geografsko podneblje za uzorkovanje su odabirani stacionarni pčelinjaci u koje idealno nisu donošene matice sa strane tokom dužeg vremenskog perioda.
Cilj projekta SERBHIWE je da se definiše genetička struktura populacije medonosnih pčela u Srbiji, kao i da se na genetičkom nivou potencijalno prepoznaju lokalno adaptirane podvrste, ekotipovi i populacije. Sekvenciranjem COI-COII intergenskog regiona kod pčela iz Srbije zabeleženo je 20 mitohondrijskih haplotipova od kojih je čak 10 novih za nauku. Najčešće detektovani haplotip pripada liniji C2d, i pronañen je na svih 18 lokaliteta. Sledeći po učestalosti je haplotip C2e koji je otkriven kod jedinki iz 16 lokaliteta, a zatim C1a koji je zabeležen na 12 lokaliteta. Iako je zabeležen značajna genetička raznovrsnost, nisu uočene visoke genetičke razlike koje bi jsano odvojile populacije iz različitih delova Srbije. Dodatno, korišćenjem mitohondrijske DNK nismo uspeli da utvrdimo postojanje lokalnih ekotipova karakterističnih samo za odreñeno podneblje.
Pored analize tRNAleu-cox2 intergenskog regiona, koristili smo i PCR-RFLP metodu kojom se jasno može izdvojiti A. m. macedonica koja sa A. m. carnica, čini dve podvrste medonosne pčele koje su kao autohtone bile zabeležene na teritoriji Republike Srbije.
Iako prethodni radovi, pokazuju prisustvo A. m. carnica u Vojvodini, i gotovo preovlañujuće prisustvo A. m. macedonica u južnoj Srbiji, sa zonom hibridizacije izmeñu, mi nismo uspeli ni u jednom od (542) uzorka da dobijemo restrikcioni obrazac karakterističan za ovu podvrstu. Razlog tome možda leži u tome što je prema zakonu o stočarstvu Republike Srbije dozvoljeno gajenje samo A. m. carnica, kao i celokupnoj promeni pčelarske prakse, gde migratorno pčelarstvo postaje sve zastupljenije, dok se proizvodnja matica razrojavanjem od sopstvenih društava sve više napušta u korist kupovine komercijalno uzgojenih matica.
PB  - Belgrade : Entomological Society of Serbia
C3  - Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi
T1  - Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije
SP  - 48
EP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5037
ER  - 

@conference{
author = "Erić, Pavle and Tanasković, Marija and Patenković, Aleksandra and Erić, Katarina and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Zapadna medonosna pčela je kosmopolit i jedan od najznačajnih oprašivača na planeti zemlji. Smatra se da je vrsta nastala na afričkom kontinentu odakle se prirodno proširila u Evropu, Bliski istok i zapadne delove Azije. Čovek je shvativši ekonomski značaj ove vrste medonosnu pčelu preneo u Severnu i Južnu Ameriku, istočnu Aziju, Australiju i Novi Zeland. Medonosna pčela se šireći svoj areal prilagoñavala novim životnim sredinama, sinhronizujući svoje aktivnosti sa ciklusima cvetanja lokalnih biljaka, kao i sa klimatskim faktorima u novim sredinama, pa tako imamo 33 opisane podvrste Apis mellifera. Ove podvrste podeljene su u 5 evolutivnih linija označenih sa A, M, C, O i Y, korišćenjem morfometrijskih kao i genetičkih markera. Linija A je karakteristična za Afrički kontinent, dok su M i C karakteristične za Severozapadnu i Jugoistočnu Evropu. Linije O i Y karakteristične su za Bliski istok.
U okviru programa za izvrsne projekte mladih istraživača (PROMIS), finansiranog od strane fonda za nauku Republike Srbije realizovan je projekat istraživanja genetičke raznovrsnosti gajenih i divljih društava medonosne pčele Apis mellifera (SERBHIWE). Tokom 2020 i 2021 godine, organizovan je terenski rad na kome su za genetička istraživanja uzorkovani pčelinjaci poreklom sa juga, severa, istoka i zapada Srbije. Uporedo su uzorkovani i pčelinjaci sa teritorije Beograda. Za vreme trajanja projekta uzorkovano je ukupno 102 pčelinjaka i 930 pčelinjih društava (košnica), od kojih je 542 odabrano za analizu genetičke raznovrsnosti mitohondrijske DNK.
Kako bi što bolje prezentovali genetičku raznovrsnost karakterističnu za lokalno geografsko podneblje za uzorkovanje su odabirani stacionarni pčelinjaci u koje idealno nisu donošene matice sa strane tokom dužeg vremenskog perioda.
Cilj projekta SERBHIWE je da se definiše genetička struktura populacije medonosnih pčela u Srbiji, kao i da se na genetičkom nivou potencijalno prepoznaju lokalno adaptirane podvrste, ekotipovi i populacije. Sekvenciranjem COI-COII intergenskog regiona kod pčela iz Srbije zabeleženo je 20 mitohondrijskih haplotipova od kojih je čak 10 novih za nauku. Najčešće detektovani haplotip pripada liniji C2d, i pronañen je na svih 18 lokaliteta. Sledeći po učestalosti je haplotip C2e koji je otkriven kod jedinki iz 16 lokaliteta, a zatim C1a koji je zabeležen na 12 lokaliteta. Iako je zabeležen značajna genetička raznovrsnost, nisu uočene visoke genetičke razlike koje bi jsano odvojile populacije iz različitih delova Srbije. Dodatno, korišćenjem mitohondrijske DNK nismo uspeli da utvrdimo postojanje lokalnih ekotipova karakterističnih samo za odreñeno podneblje.
Pored analize tRNAleu-cox2 intergenskog regiona, koristili smo i PCR-RFLP metodu kojom se jasno može izdvojiti A. m. macedonica koja sa A. m. carnica, čini dve podvrste medonosne pčele koje su kao autohtone bile zabeležene na teritoriji Republike Srbije.
Iako prethodni radovi, pokazuju prisustvo A. m. carnica u Vojvodini, i gotovo preovlañujuće prisustvo A. m. macedonica u južnoj Srbiji, sa zonom hibridizacije izmeñu, mi nismo uspeli ni u jednom od (542) uzorka da dobijemo restrikcioni obrazac karakterističan za ovu podvrstu. Razlog tome možda leži u tome što je prema zakonu o stočarstvu Republike Srbije dozvoljeno gajenje samo A. m. carnica, kao i celokupnoj promeni pčelarske prakse, gde migratorno pčelarstvo postaje sve zastupljenije, dok se proizvodnja matica razrojavanjem od sopstvenih društava sve više napušta u korist kupovine komercijalno uzgojenih matica.",
publisher = "Belgrade : Entomological Society of Serbia",
journal = "Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi",
title = "Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije",
pages = "48-49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5037"
}

Erić, P., Tanasković, M., Patenković, A., Erić, K., Stanisavljević, L.,& Davidović, S.. (2022). Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi
Belgrade : Entomological Society of Serbia., 48-49.
https://hdl.handle.net/21.15107/rcub_ibiss_5037

Erić P, Tanasković M, Patenković A, Erić K, Stanisavljević L, Davidović S. Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi. 2022;:48-49.
https://hdl.handle.net/21.15107/rcub_ibiss_5037 .

Erić, Pavle, Tanasković, Marija, Patenković, Aleksandra, Erić, Katarina, Stanisavljević, Ljubiša, Davidović, Slobodan, "Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije" in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi (2022):48-49,
https://hdl.handle.net/21.15107/rcub_ibiss_5037 .

TY  - CONF
AU  - Erić, Pavle
AU  - Tanasković, Marija
AU  - Erić, Katarina
AU  - Patenković, Aleksandra
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - https://www.eseb2022.cz/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5035
AB  - The western honey bee (Apis mellifera) is one of natures’ most important pollinators and domesticated species. Through human management, it has spread all around the world becoming a cosmopolitan species, and its genetic diversity has been shaped over millennia by anthropogenic influence. Six main mtDNA lineages (M, C, O, Y, A and S) with a large number of existing sublineages are recognized, usually associated with appropriate subspecies. Current nomenclature is inconsistent and the naming of newly identified haplotypes is not standardized. Here we reassessed the nomenclature of C lineage using sequences of the tRNAleu-cox2 intergenic region and complete mitogenomes. Most of the available honey bees’ mtDNA haplotypes are based on the sequence variability of this region while available complete mitogenomes are still few. The first complete mitogenome of Apis mellifera (NC_001566) was used as the reference genome for haplotype determination. We propose that the naming of the mtDNA haplotypes should be performed based on the differences to the reference mitogenome and according to the phylogenetic relationship between detected haplotypes. Our analyses showed that the current nomenclature of C sublineages is not consistent with the phylogenetic relationships between existing haplotypes and that the reassessment followed by the proper naming of haplotypes is needed. We propose to keep the names of the main lineages but correct the names of sublineages using the same approach as in the identification of human mtDNA lineages. This newly standardized nomenclature will bring more order to the studies of honey bees’ genetic diversity.
PB  - European Society for Evolutionary Biology
C3  - Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
T1  - Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny
SP  - 753
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5035
ER  - 

@conference{
author = "Erić, Pavle and Tanasković, Marija and Erić, Katarina and Patenković, Aleksandra and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "The western honey bee (Apis mellifera) is one of natures’ most important pollinators and domesticated species. Through human management, it has spread all around the world becoming a cosmopolitan species, and its genetic diversity has been shaped over millennia by anthropogenic influence. Six main mtDNA lineages (M, C, O, Y, A and S) with a large number of existing sublineages are recognized, usually associated with appropriate subspecies. Current nomenclature is inconsistent and the naming of newly identified haplotypes is not standardized. Here we reassessed the nomenclature of C lineage using sequences of the tRNAleu-cox2 intergenic region and complete mitogenomes. Most of the available honey bees’ mtDNA haplotypes are based on the sequence variability of this region while available complete mitogenomes are still few. The first complete mitogenome of Apis mellifera (NC_001566) was used as the reference genome for haplotype determination. We propose that the naming of the mtDNA haplotypes should be performed based on the differences to the reference mitogenome and according to the phylogenetic relationship between detected haplotypes. Our analyses showed that the current nomenclature of C sublineages is not consistent with the phylogenetic relationships between existing haplotypes and that the reassessment followed by the proper naming of haplotypes is needed. We propose to keep the names of the main lineages but correct the names of sublineages using the same approach as in the identification of human mtDNA lineages. This newly standardized nomenclature will bring more order to the studies of honey bees’ genetic diversity.",
publisher = "European Society for Evolutionary Biology",
journal = "Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic",
title = "Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny",
pages = "753",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5035"
}

Erić, P., Tanasković, M., Erić, K., Patenković, A., Stanisavljević, L.,& Davidović, S.. (2022). Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny. in Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
European Society for Evolutionary Biology., 753.
https://hdl.handle.net/21.15107/rcub_ibiss_5035

Erić P, Tanasković M, Erić K, Patenković A, Stanisavljević L, Davidović S. Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny. in Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic. 2022;:753.
https://hdl.handle.net/21.15107/rcub_ibiss_5035 .

Erić, Pavle, Tanasković, Marija, Erić, Katarina, Patenković, Aleksandra, Stanisavljević, Ljubiša, Davidović, Slobodan, "Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny" in Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic (2022):753,
https://hdl.handle.net/21.15107/rcub_ibiss_5035 .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Erić, Pavle
AU  - Stanisavljević, Ljubiša
AU  - Tanasković, Marija
PY  - 2022
UR  - https://eurbee9.bio.bg.ac.rs/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5038
AB  - The honey bee is one of the most economically and ecologically important species currently facing serious challenges. Its history is marked by strong anthropogenic influence and its local populations are rapidly changing while its diversity is constantly manipulated by beekeepers through the import of foreign queens, selection, and migratory beekeeping. The practice of queen importation caused one of the most disastrous events in the history of beekeeping when in the mid-20th century Varroa mite was accidentally imported to Europe. This infestation, combined with the degradation of natural habitats, was believed to have caused the disappearance of free-living populations throughout European forests. Recent studies challenge this view and demonstrate that honey bees are able to survive in nature without human interference.
To assess the genetic diversity of different honey bee colonies, we analyzed mitochondrial DNA and 14 microsatellite loci in samples collected from 102 apiaries (with 542 hives) and 55 free-living colonies (FC) from the urban and natural environments throughout Serbia.
Standard parameters of genetic diversity for both genetic markers showed that FC exhibit higher values compared to managed colonies (MC). Values of pairwise F ST genetic distances were higher when FC were compared to MC from different parts of Serbia while MC exhibited somewhat lower values when compared among themselves. Relatedness estimates demonstrated that feral colonies are more related among themselves than they are to other MC and vice versa, implying the existence of a viable and stable free-living population of honey bees. A significant change in the distribution and diversity of mtDNA lineages among MC compared to the historical data for Serbia was detected while genetic analysis based on microsatellite data demonstrated a high genetic uniformity.
Our data confirmed the existence of a genetically diverse and distinctive free-living population of honey bees in Serbia worthy of protection as well as a significant change in the genetic diversity of managed honey bees that calls for immediate change in the current beekeeping practices so that genetic diversity of locally adapted populations could be preserved.
PB  - European Association for Bee Research
C3  - Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia
T1  - Free-living vs. managed honey bees – a population genetic approach
SP  - 48
EP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5038
ER  - 

@conference{
author = "Davidović, Slobodan and Patenković, Aleksandra and Erić, Katarina and Erić, Pavle and Stanisavljević, Ljubiša and Tanasković, Marija",
year = "2022",
abstract = "The honey bee is one of the most economically and ecologically important species currently facing serious challenges. Its history is marked by strong anthropogenic influence and its local populations are rapidly changing while its diversity is constantly manipulated by beekeepers through the import of foreign queens, selection, and migratory beekeeping. The practice of queen importation caused one of the most disastrous events in the history of beekeeping when in the mid-20th century Varroa mite was accidentally imported to Europe. This infestation, combined with the degradation of natural habitats, was believed to have caused the disappearance of free-living populations throughout European forests. Recent studies challenge this view and demonstrate that honey bees are able to survive in nature without human interference.
To assess the genetic diversity of different honey bee colonies, we analyzed mitochondrial DNA and 14 microsatellite loci in samples collected from 102 apiaries (with 542 hives) and 55 free-living colonies (FC) from the urban and natural environments throughout Serbia.
Standard parameters of genetic diversity for both genetic markers showed that FC exhibit higher values compared to managed colonies (MC). Values of pairwise F ST genetic distances were higher when FC were compared to MC from different parts of Serbia while MC exhibited somewhat lower values when compared among themselves. Relatedness estimates demonstrated that feral colonies are more related among themselves than they are to other MC and vice versa, implying the existence of a viable and stable free-living population of honey bees. A significant change in the distribution and diversity of mtDNA lineages among MC compared to the historical data for Serbia was detected while genetic analysis based on microsatellite data demonstrated a high genetic uniformity.
Our data confirmed the existence of a genetically diverse and distinctive free-living population of honey bees in Serbia worthy of protection as well as a significant change in the genetic diversity of managed honey bees that calls for immediate change in the current beekeeping practices so that genetic diversity of locally adapted populations could be preserved.",
publisher = "European Association for Bee Research",
journal = "Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia",
title = "Free-living vs. managed honey bees – a population genetic approach",
pages = "48-49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5038"
}

Davidović, S., Patenković, A., Erić, K., Erić, P., Stanisavljević, L.,& Tanasković, M.. (2022). Free-living vs. managed honey bees – a population genetic approach. in Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia
European Association for Bee Research., 48-49.
https://hdl.handle.net/21.15107/rcub_ibiss_5038

Davidović S, Patenković A, Erić K, Erić P, Stanisavljević L, Tanasković M. Free-living vs. managed honey bees – a population genetic approach. in Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia. 2022;:48-49.
https://hdl.handle.net/21.15107/rcub_ibiss_5038 .

Davidović, Slobodan, Patenković, Aleksandra, Erić, Katarina, Erić, Pavle, Stanisavljević, Ljubiša, Tanasković, Marija, "Free-living vs. managed honey bees – a population genetic approach" in Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia (2022):48-49,
https://hdl.handle.net/21.15107/rcub_ibiss_5038 .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Patenković, Aleksandra
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Stanisavljević, Ljubiša
AU  - Tanasković, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5036
AB  - Zbog svoje uloge oprašivača kao i proizvoda koji se dobijaju od nje, medonosna pčela je prepoznata kao jedna od najvažnijih uzgajanih vrsta životinja. Trenutno je poznato 33 podvrsta koje su karakteristične za regione u kojima se uzgajaju. Za Srbiju su bile autohtone dve podvrste: Apis mellifera carnica i Apis mellifera macedonica. Meñutim, u poslednjih dvanaest godina došlo je do nagle ekspanzije pčelarstva koje je pratilo povećanje broja pčelara, košnica i broja selekcionih centara za uzgoj matica što je uslovilo značajne promene u genetičkoj raznovrsnosti ove važne vrste. Za potrebe molekularno genetičkih analiza, a radi utvrñivanja genetičke strukture populacije uzgajanih medonosnih pčela, uzorkovane su pčele radlilice iz 542 košnice poreklom iz 102 stacionarna pčelinjaka širom Srbije. Genetička raznovrsnost je procenjena analizom varijabilnosti mikrosatelitnih ponovaka iz 14 lokusa. Razlikovanje podvrste A. m. carnica od A. m. macedonica rañeno je na osnovu analize specifičnog polimorfizma u mitohondrijskoj DNK.
U sakupljenom uzorku nije detektovano prisustvo mtDNK linije karakteristične za podvrstu A. m. macedonica što ukazuje na gubitak jedne od autohtonih podvrsta. Vrednosti parametara genetičke raznovrsnosti su za većinu analiziranih regiona ujednačeni dok je očekivana heterozigotnost kod svih regiona viša od opažene. Najveća prosečna vrednost za raznovrsnost gena po lokusima zapažena je za Sjeničko-Pešterski region dok je najveći prosečni broj alela detektovan u okolini Leskovca. Mera genetičke udaljenosti, FST, izmeñu parova analiziranih populacija je pokazala da se pčele poreklom iz Sjeničko-Pešterskog regiona najviše razlikuju dok su pčele poreklom iz ostalih regiona meñusobno sličnije. Dodatno, diskriminativna analiza glavnih komponenti (DAPC) je pokazala visoku stopu preklapanja jedinki poreklom iz različitih regiona. Ovi podaci ukazuju na visok stepen protoka gena izmeñu različitih regiona Srbije koji su geografski udaljeni i imaju drugačije uslove životne sredine. Dobijeni rezultat je najverovatnije posledica savremenih pčelarskih praksi koje podrazumevaju migratorno pčelarstvo i kupovinu komercijlano dostupnih matica. Opisani način održavanja pčelinjaka dovodi do kontakta i protoka gena izmeñu pčela poreklom iz različitih regiona što nije slučaj u prirodnim uslovima ili prilikom tradicionalnog načina održavanja stacionarnih pčelinjaka uz praksu razrojavanja i samostalnu proizvodnju matica.
Sprovedene analize ukazuju na gubitak genetičke raznovrsnosti kod lokalno adaptiranih populacija kao i gubitak jedne od dve podvrste medonosnih pčela autohtonih na teritoriji Srbije. Trenutna situacija zahteva preispitivanje savremenih praksi koje se primenjuju u pčelarstvu i primenu adekvatnih mera kako bi se očuvala genetička raznovrsnost.
PB  - Belgrade : Entomological Society of Serbia
C3  - Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.
T1  - Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije
SP  - 47
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5036
ER  - 

@conference{
author = "Davidović, Slobodan and Patenković, Aleksandra and Erić, Pavle and Erić, Katarina and Stanisavljević, Ljubiša and Tanasković, Marija",
year = "2022",
abstract = "Zbog svoje uloge oprašivača kao i proizvoda koji se dobijaju od nje, medonosna pčela je prepoznata kao jedna od najvažnijih uzgajanih vrsta životinja. Trenutno je poznato 33 podvrsta koje su karakteristične za regione u kojima se uzgajaju. Za Srbiju su bile autohtone dve podvrste: Apis mellifera carnica i Apis mellifera macedonica. Meñutim, u poslednjih dvanaest godina došlo je do nagle ekspanzije pčelarstva koje je pratilo povećanje broja pčelara, košnica i broja selekcionih centara za uzgoj matica što je uslovilo značajne promene u genetičkoj raznovrsnosti ove važne vrste. Za potrebe molekularno genetičkih analiza, a radi utvrñivanja genetičke strukture populacije uzgajanih medonosnih pčela, uzorkovane su pčele radlilice iz 542 košnice poreklom iz 102 stacionarna pčelinjaka širom Srbije. Genetička raznovrsnost je procenjena analizom varijabilnosti mikrosatelitnih ponovaka iz 14 lokusa. Razlikovanje podvrste A. m. carnica od A. m. macedonica rañeno je na osnovu analize specifičnog polimorfizma u mitohondrijskoj DNK.
U sakupljenom uzorku nije detektovano prisustvo mtDNK linije karakteristične za podvrstu A. m. macedonica što ukazuje na gubitak jedne od autohtonih podvrsta. Vrednosti parametara genetičke raznovrsnosti su za većinu analiziranih regiona ujednačeni dok je očekivana heterozigotnost kod svih regiona viša od opažene. Najveća prosečna vrednost za raznovrsnost gena po lokusima zapažena je za Sjeničko-Pešterski region dok je najveći prosečni broj alela detektovan u okolini Leskovca. Mera genetičke udaljenosti, FST, izmeñu parova analiziranih populacija je pokazala da se pčele poreklom iz Sjeničko-Pešterskog regiona najviše razlikuju dok su pčele poreklom iz ostalih regiona meñusobno sličnije. Dodatno, diskriminativna analiza glavnih komponenti (DAPC) je pokazala visoku stopu preklapanja jedinki poreklom iz različitih regiona. Ovi podaci ukazuju na visok stepen protoka gena izmeñu različitih regiona Srbije koji su geografski udaljeni i imaju drugačije uslove životne sredine. Dobijeni rezultat je najverovatnije posledica savremenih pčelarskih praksi koje podrazumevaju migratorno pčelarstvo i kupovinu komercijlano dostupnih matica. Opisani način održavanja pčelinjaka dovodi do kontakta i protoka gena izmeñu pčela poreklom iz različitih regiona što nije slučaj u prirodnim uslovima ili prilikom tradicionalnog načina održavanja stacionarnih pčelinjaka uz praksu razrojavanja i samostalnu proizvodnju matica.
Sprovedene analize ukazuju na gubitak genetičke raznovrsnosti kod lokalno adaptiranih populacija kao i gubitak jedne od dve podvrste medonosnih pčela autohtonih na teritoriji Srbije. Trenutna situacija zahteva preispitivanje savremenih praksi koje se primenjuju u pčelarstvu i primenu adekvatnih mera kako bi se očuvala genetička raznovrsnost.",
publisher = "Belgrade : Entomological Society of Serbia",
journal = "Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.",
title = "Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije",
pages = "47-48",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5036"
}

Davidović, S., Patenković, A., Erić, P., Erić, K., Stanisavljević, L.,& Tanasković, M.. (2022). Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.
Belgrade : Entomological Society of Serbia., 47-48.
https://hdl.handle.net/21.15107/rcub_ibiss_5036

Davidović S, Patenković A, Erić P, Erić K, Stanisavljević L, Tanasković M. Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.. 2022;:47-48.
https://hdl.handle.net/21.15107/rcub_ibiss_5036 .

Davidović, Slobodan, Patenković, Aleksandra, Erić, Pavle, Erić, Katarina, Stanisavljević, Ljubiša, Tanasković, Marija, "Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije" in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia. (2022):47-48,
https://hdl.handle.net/21.15107/rcub_ibiss_5036 .

TY  - DATA
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Kusza, Szilvia
AU  - Oleksa, Andzrej
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4779
AB  - Figure S1. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 14 microsatellite loci (A7, A8, A14, A24, A28, A35, A43, A79, A88, A107, A113, Ap43, Ap249 and B124) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries and 8 different localities from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica) and South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia. In figure S1 the first three principal components (PC) are presented.
Figure S7. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 9 microsatellite loci (A7, A14, A24, A28, A35, A43, A88, A107 and A113) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries and 8 different localities from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica) and South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia and four different European countries (Spain, Italy, Poland and Hungary). In this figure four different A. mellifera species are presented as well: A. m. iberica from Spain, A. m. ligustica from Italy, A. m. mellifera from Poland, A. m. carnica from Hungary, Poland and Serbia. In figure S7 the first three principal components (PC) are presented.
T2  - Insects
T1  - Supplementary material "Further evidence of population admixture in the Serbian honey bee population"
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4779
ER  - 

@misc{
author = "Tanasković, Marija and Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Mihajlović, Milica and Tanasić, Vanja and Kusza, Szilvia and Oleksa, Andzrej and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Figure S1. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 14 microsatellite loci (A7, A8, A14, A24, A28, A35, A43, A79, A88, A107, A113, Ap43, Ap249 and B124) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries and 8 different localities from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica) and South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia. In figure S1 the first three principal components (PC) are presented.
Figure S7. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 9 microsatellite loci (A7, A14, A24, A28, A35, A43, A88, A107 and A113) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries and 8 different localities from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica) and South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia and four different European countries (Spain, Italy, Poland and Hungary). In this figure four different A. mellifera species are presented as well: A. m. iberica from Spain, A. m. ligustica from Italy, A. m. mellifera from Poland, A. m. carnica from Hungary, Poland and Serbia. In figure S7 the first three principal components (PC) are presented.",
journal = "Insects",
title = "Supplementary material "Further evidence of population admixture in the Serbian honey bee population"",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4779"
}

Tanasković, M., Erić, P., Patenković, A., Erić, K., Mihajlović, M., Tanasić, V., Kusza, S., Oleksa, A., Stanisavljević, L.,& Davidović, S.. (2022). Supplementary material "Further evidence of population admixture in the Serbian honey bee population". in Insects.
https://hdl.handle.net/21.15107/rcub_ibiss_4779

Tanasković M, Erić P, Patenković A, Erić K, Mihajlović M, Tanasić V, Kusza S, Oleksa A, Stanisavljević L, Davidović S. Supplementary material "Further evidence of population admixture in the Serbian honey bee population". in Insects. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_4779 .

Tanasković, Marija, Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Mihajlović, Milica, Tanasić, Vanja, Kusza, Szilvia, Oleksa, Andzrej, Stanisavljević, Ljubiša, Davidović, Slobodan, "Supplementary material "Further evidence of population admixture in the Serbian honey bee population"" in Insects (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_4779 .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Jelić, Mihailo
AU  - Marinković, Saša
AU  - Kukobat, Mila
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Hribšek, Irena
AU  - Sušić, Goran
AU  - Dragićević, Milan
AU  - Tanasković, Marija
AU  - Stamenković-Radak, Marina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4254
AB  - Abstract
Once a widespread species across the region of Southeast Europe, the Griffon vulture is now confined to small and isolated populations across the Balkan Peninsula. The population from Serbia represents its biggest and most viable population that can serve as an important reservoir of genetic diversity from which the birds can be used for the region’s reintroduction programs. The available genetic data for this valuable population are scarce and it is necessary to assess its genetic diversity and inbreeding level if the population is going to be used for restocking and reintroduction.
To assess the genetic diversity we used microsatellite markers from ten loci and mitochondrial Cytb nucleotide sequences. The blood samples were collected from 58 unrelated birds during the marking in the nests. We have performed a comparative analysis of newly obtained data on microsatellites and Cytb with existing data. Genetic differentiation analysis between different native populations of French Pyrenees, Croatia and Israel identified two genetic clusters that differentiate populations from the Balkan and Iberian Peninsulas. Genetic diversity analysis based on microsatellites demonstrated similar levels among all populations while analysis of Cytb detected somewhat lower diversity in the population from Serbia. Further analyses demonstrated that all analyzed populations experienced a recent bottleneck event. Phylogeographic analysis based on Cytb sequences showed that the most frequent haplotype is found in all Griffon vulture populations and that each population possesses private haplotypes. Considering the serious recent bottleneck event which the population from Serbia experienced we estimated the overall relatedness among the birds from this population. The level of inbreeding was relatively high and on average it was 8,3% while the mean number of relatives for each bird was close to three.
Our data suggest that, even though a relatively high level of inbreeding can be detected among the individual birds, the Griffon vulture population from Serbia can be used as a source population for restocking and reintroduction programs in the region. The observed genetic differentiation between the populations from the Iberian and Balkan Peninsula suggest that the introduction of foreign birds should be avoided and that local birds should be used instead.
PB  - Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
C3  - Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia
T1  - Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia
IS  - 1
VL  - 43
SP  - 117
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4254
ER  - 

@conference{
author = "Davidović, Slobodan and Jelić, Mihailo and Marinković, Saša and Kukobat, Mila and Mihajlović, Milica and Tanasić, Vanja and Hribšek, Irena and Sušić, Goran and Dragićević, Milan and Tanasković, Marija and Stamenković-Radak, Marina",
year = "2021",
abstract = "Abstract
Once a widespread species across the region of Southeast Europe, the Griffon vulture is now confined to small and isolated populations across the Balkan Peninsula. The population from Serbia represents its biggest and most viable population that can serve as an important reservoir of genetic diversity from which the birds can be used for the region’s reintroduction programs. The available genetic data for this valuable population are scarce and it is necessary to assess its genetic diversity and inbreeding level if the population is going to be used for restocking and reintroduction.
To assess the genetic diversity we used microsatellite markers from ten loci and mitochondrial Cytb nucleotide sequences. The blood samples were collected from 58 unrelated birds during the marking in the nests. We have performed a comparative analysis of newly obtained data on microsatellites and Cytb with existing data. Genetic differentiation analysis between different native populations of French Pyrenees, Croatia and Israel identified two genetic clusters that differentiate populations from the Balkan and Iberian Peninsulas. Genetic diversity analysis based on microsatellites demonstrated similar levels among all populations while analysis of Cytb detected somewhat lower diversity in the population from Serbia. Further analyses demonstrated that all analyzed populations experienced a recent bottleneck event. Phylogeographic analysis based on Cytb sequences showed that the most frequent haplotype is found in all Griffon vulture populations and that each population possesses private haplotypes. Considering the serious recent bottleneck event which the population from Serbia experienced we estimated the overall relatedness among the birds from this population. The level of inbreeding was relatively high and on average it was 8,3% while the mean number of relatives for each bird was close to three.
Our data suggest that, even though a relatively high level of inbreeding can be detected among the individual birds, the Griffon vulture population from Serbia can be used as a source population for restocking and reintroduction programs in the region. The observed genetic differentiation between the populations from the Iberian and Balkan Peninsula suggest that the introduction of foreign birds should be avoided and that local birds should be used instead.",
publisher = "Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad",
journal = "Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia",
title = "Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia",
number = "1",
volume = "43",
pages = "117",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4254"
}

Davidović, S., Jelić, M., Marinković, S., Kukobat, M., Mihajlović, M., Tanasić, V., Hribšek, I., Sušić, G., Dragićević, M., Tanasković, M.,& Stamenković-Radak, M.. (2021). Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia. in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia
Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad., 43(1), 117.
https://hdl.handle.net/21.15107/rcub_ibiss_4254

Davidović S, Jelić M, Marinković S, Kukobat M, Mihajlović M, Tanasić V, Hribšek I, Sušić G, Dragićević M, Tanasković M, Stamenković-Radak M. Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia. in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia. 2021;43(1):117.
https://hdl.handle.net/21.15107/rcub_ibiss_4254 .

Davidović, Slobodan, Jelić, Mihailo, Marinković, Saša, Kukobat, Mila, Mihajlović, Milica, Tanasić, Vanja, Hribšek, Irena, Sušić, Goran, Dragićević, Milan, Tanasković, Marija, Stamenković-Radak, Marina, "Genetic diversity analysis of microsatellites and mitochondrial Cytb gene, relatedness estimates and Cytb phylogeography of protected Griffon vulture species from Serbia" in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia, 43, no. 1 (2021):117,
https://hdl.handle.net/21.15107/rcub_ibiss_4254 .