TY  - JOUR
AU  - Martinovic, Rajko
AU  - Kolarevic, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Kostic, Jovana
AU  - Markovic, Sandra
AU  - Gacic, Zoran
AU  - Kljajic, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1936
AB  - The effects of ex situ exposure of Mytilus galloprovincialis to
   Superdispersant-25 (S-25), diesel oil and dispersed diesel oil mixtures
   were studied by the impact on level of DNA damage in haemocytes (comet
   assay) and the cardiac activity patterns of mussels. Specimens were
   exposed for 72 h in a static system to diesel oil (100 mu L/L and 1
   mL/L), S-25 (5 and 50 mu L/L), and dispersed diesel oil mixtures M1
   (diesel oil 100 g mu L/L + S-25 5 mu L/L) and M2 (diesel oil 1 mL/L +
   S-25 50 mu L/L). For positive control 40 mu M CdCl2 was used. The comet
   assay results indicated genotoxic potential of S-25 while the effects of
   diesel oil alone were not observed. The highest response was detected
   for M1 while the effects of M2 were not detected. The heart rate
   disorders were recorded for the diesel oil (1 mL/L), S-25 (50 mu L/L)
   and both dispersed diesel oil mixtures. (C) 2015 Elsevier Ltd. All
   rights reserved.
T2  - Marine Environmental Research
T1  - Genotoxic potential and heart rate disorders in the Mediterranean mussel
 Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed
 diesel oil
VL  - 108
DO  - 10.1016/j.marenvres.2015.05.001
SP  - 83
EP  - 90
ER  - 

@article{
author = "Martinovic, Rajko and Kolarevic, Stoimir and Kračun-Kolarević, Margareta and Kostic, Jovana and Markovic, Sandra and Gacic, Zoran and Kljajic, Zoran and Vukovic-Gacic, Branka",
year = "2015",
abstract = "The effects of ex situ exposure of Mytilus galloprovincialis to
   Superdispersant-25 (S-25), diesel oil and dispersed diesel oil mixtures
   were studied by the impact on level of DNA damage in haemocytes (comet
   assay) and the cardiac activity patterns of mussels. Specimens were
   exposed for 72 h in a static system to diesel oil (100 mu L/L and 1
   mL/L), S-25 (5 and 50 mu L/L), and dispersed diesel oil mixtures M1
   (diesel oil 100 g mu L/L + S-25 5 mu L/L) and M2 (diesel oil 1 mL/L +
   S-25 50 mu L/L). For positive control 40 mu M CdCl2 was used. The comet
   assay results indicated genotoxic potential of S-25 while the effects of
   diesel oil alone were not observed. The highest response was detected
   for M1 while the effects of M2 were not detected. The heart rate
   disorders were recorded for the diesel oil (1 mL/L), S-25 (50 mu L/L)
   and both dispersed diesel oil mixtures. (C) 2015 Elsevier Ltd. All
   rights reserved.",
journal = "Marine Environmental Research",
title = "Genotoxic potential and heart rate disorders in the Mediterranean mussel
 Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed
 diesel oil",
volume = "108",
doi = "10.1016/j.marenvres.2015.05.001",
pages = "83-90"
}

Martinovic, R., Kolarevic, S., Kračun-Kolarević, M., Kostic, J., Markovic, S., Gacic, Z., Kljajic, Z.,& Vukovic-Gacic, B.. (2015). Genotoxic potential and heart rate disorders in the Mediterranean mussel
 Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed
 diesel oil. in Marine Environmental Research, 108, 83-90.
https://doi.org/10.1016/j.marenvres.2015.05.001

Martinovic R, Kolarevic S, Kračun-Kolarević M, Kostic J, Markovic S, Gacic Z, Kljajic Z, Vukovic-Gacic B. Genotoxic potential and heart rate disorders in the Mediterranean mussel
 Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed
 diesel oil. in Marine Environmental Research. 2015;108:83-90.
doi:10.1016/j.marenvres.2015.05.001 .

Martinovic, Rajko, Kolarevic, Stoimir, Kračun-Kolarević, Margareta, Kostic, Jovana, Markovic, Sandra, Gacic, Zoran, Kljajic, Zoran, Vukovic-Gacic, Branka, "Genotoxic potential and heart rate disorders in the Mediterranean mussel
 Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed
 diesel oil" in Marine Environmental Research, 108 (2015):83-90,
https://doi.org/10.1016/j.marenvres.2015.05.001 . .

TY  - JOUR
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Visnjic-Jeftic, Zeljka
AU  - Skoric, Stefan
AU  - Gacic, Zoran
AU  - Đikanović, Vesna
AU  - Jaric, Ivan
AU  - Lenhardt, Mirjana
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2368
AB  - The bottom feeding fish species have a good potential to be used for
   assessments of pollution, as they are under pressure from pollutants
   from both water and sediments. In this study, the level of similarity of
   histopathological responses to pollution in gills and liver between
   barbel (Barbus barbus) and sterlet (Acipenser ruthenus) from the Danube
   River was assessed, and compared with elemental concentrations in their
   gills, liver, and muscle. Results indicate that the detected metal
   concentrations were likely cause of different tissue responses in gills
   and liver of the two investigated fish species. Statistical analysis
   indicated a clear differentiation of the two species based on elemental
   concentrations and the level of histopathological changes in gills and
   liver. Metal concentrations exceeded maximum acceptable concentrations
   in a number of analyzed specimens, which indicates the importance of
   this type of monitoring. Results indicate that barbel is a better
   indicator for specific, rather narrow sites, whereas sterlet is a better
   indicator of larger (longer) water current segments. Obtained
   information could be of importance for both scientists and fishery and
   water management authorities working on the development of water
   monitoring programs. (c) 2014 Wiley Periodicals, Inc. Environ Toxicol
   30: 1153-1161, 2015.
T2  - Environmental Toxicology
T1  - Use of histopathology and elemental accumulation in different organs of
 two benthophagous fish species as indicators of river pollution
IS  - 10
VL  - 30
DO  - 10.1002/tox.21988
SP  - 1153
EP  - 1161
ER  - 

@article{
author = "Raskovic, Bozidar and Poleksic, Vesna and Visnjic-Jeftic, Zeljka and Skoric, Stefan and Gacic, Zoran and Đikanović, Vesna and Jaric, Ivan and Lenhardt, Mirjana",
year = "2015",
abstract = "The bottom feeding fish species have a good potential to be used for
   assessments of pollution, as they are under pressure from pollutants
   from both water and sediments. In this study, the level of similarity of
   histopathological responses to pollution in gills and liver between
   barbel (Barbus barbus) and sterlet (Acipenser ruthenus) from the Danube
   River was assessed, and compared with elemental concentrations in their
   gills, liver, and muscle. Results indicate that the detected metal
   concentrations were likely cause of different tissue responses in gills
   and liver of the two investigated fish species. Statistical analysis
   indicated a clear differentiation of the two species based on elemental
   concentrations and the level of histopathological changes in gills and
   liver. Metal concentrations exceeded maximum acceptable concentrations
   in a number of analyzed specimens, which indicates the importance of
   this type of monitoring. Results indicate that barbel is a better
   indicator for specific, rather narrow sites, whereas sterlet is a better
   indicator of larger (longer) water current segments. Obtained
   information could be of importance for both scientists and fishery and
   water management authorities working on the development of water
   monitoring programs. (c) 2014 Wiley Periodicals, Inc. Environ Toxicol
   30: 1153-1161, 2015.",
journal = "Environmental Toxicology",
title = "Use of histopathology and elemental accumulation in different organs of
 two benthophagous fish species as indicators of river pollution",
number = "10",
volume = "30",
doi = "10.1002/tox.21988",
pages = "1153-1161"
}

Raskovic, B., Poleksic, V., Visnjic-Jeftic, Z., Skoric, S., Gacic, Z., Đikanović, V., Jaric, I.,& Lenhardt, M.. (2015). Use of histopathology and elemental accumulation in different organs of
 two benthophagous fish species as indicators of river pollution. in Environmental Toxicology, 30(10), 1153-1161.
https://doi.org/10.1002/tox.21988

Raskovic B, Poleksic V, Visnjic-Jeftic Z, Skoric S, Gacic Z, Đikanović V, Jaric I, Lenhardt M. Use of histopathology and elemental accumulation in different organs of
 two benthophagous fish species as indicators of river pollution. in Environmental Toxicology. 2015;30(10):1153-1161.
doi:10.1002/tox.21988 .

Raskovic, Bozidar, Poleksic, Vesna, Visnjic-Jeftic, Zeljka, Skoric, Stefan, Gacic, Zoran, Đikanović, Vesna, Jaric, Ivan, Lenhardt, Mirjana, "Use of histopathology and elemental accumulation in different organs of
 two benthophagous fish species as indicators of river pollution" in Environmental Toxicology, 30, no. 10 (2015):1153-1161,
https://doi.org/10.1002/tox.21988 . .

TY  - JOUR
AU  - Jacimovic, Milica
AU  - Lenhardt, Mirjana
AU  - Visnjic-Jeftic, Zeljka
AU  - Jaric, Ivan
AU  - Gacic, Zoran
AU  - Hegedis, Aleksandar
AU  - Krpo-Cetkovic, Jasmina
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2060
AB  - Distribution of 17 chemical elements in gills, muscle, and liver of the
   European perch (Perca fluviatilis) and black bullhead (Ameiurus melas)
   from the Sava Lake (Serbia) was studied to detect bioaccumulation
   patterns in relation to the species' diet and trophic level.
   Concentrations of Ba, Cd, Co, Cr, Li, Ni, Pb, B, and Se were below the
   detection limits. Concentrations of Al, Fe, Mn, Sr, and Zn were higher
   in gills of the black bullhead; As, Mo, and Sr were higher in liver of
   the European perch; Fe and Zn were higher in liver of the black
   bullhead. In muscle, a significant difference between species was found
   only for Sr. Copper was detected only in liver of the black bullhead.
   Similarity in elemental concentrations in both species could be
   explained by a relatively similar diet of these two species, while the
   differences, especially in gills, could be explained by different
   habitat preferences.
T2  - Slovenian Veterinary Research
T1  - ELEMENTAL CONCENTRATIONS IN DIFFERENT TISSUES OF EUROPEAN PERCH AND
 BLACK BULLHEAD FROM SAVA LAKE (SERBIA)
IS  - 2
VL  - 52
SP  - 57
EP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2060
ER  - 

@article{
author = "Jacimovic, Milica and Lenhardt, Mirjana and Visnjic-Jeftic, Zeljka and Jaric, Ivan and Gacic, Zoran and Hegedis, Aleksandar and Krpo-Cetkovic, Jasmina",
year = "2015",
abstract = "Distribution of 17 chemical elements in gills, muscle, and liver of the
   European perch (Perca fluviatilis) and black bullhead (Ameiurus melas)
   from the Sava Lake (Serbia) was studied to detect bioaccumulation
   patterns in relation to the species' diet and trophic level.
   Concentrations of Ba, Cd, Co, Cr, Li, Ni, Pb, B, and Se were below the
   detection limits. Concentrations of Al, Fe, Mn, Sr, and Zn were higher
   in gills of the black bullhead; As, Mo, and Sr were higher in liver of
   the European perch; Fe and Zn were higher in liver of the black
   bullhead. In muscle, a significant difference between species was found
   only for Sr. Copper was detected only in liver of the black bullhead.
   Similarity in elemental concentrations in both species could be
   explained by a relatively similar diet of these two species, while the
   differences, especially in gills, could be explained by different
   habitat preferences.",
journal = "Slovenian Veterinary Research",
title = "ELEMENTAL CONCENTRATIONS IN DIFFERENT TISSUES OF EUROPEAN PERCH AND
 BLACK BULLHEAD FROM SAVA LAKE (SERBIA)",
number = "2",
volume = "52",
pages = "57-65",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2060"
}

Jacimovic, M., Lenhardt, M., Visnjic-Jeftic, Z., Jaric, I., Gacic, Z., Hegedis, A.,& Krpo-Cetkovic, J.. (2015). ELEMENTAL CONCENTRATIONS IN DIFFERENT TISSUES OF EUROPEAN PERCH AND
 BLACK BULLHEAD FROM SAVA LAKE (SERBIA). in Slovenian Veterinary Research, 52(2), 57-65.
https://hdl.handle.net/21.15107/rcub_ibiss_2060

Jacimovic M, Lenhardt M, Visnjic-Jeftic Z, Jaric I, Gacic Z, Hegedis A, Krpo-Cetkovic J. ELEMENTAL CONCENTRATIONS IN DIFFERENT TISSUES OF EUROPEAN PERCH AND
 BLACK BULLHEAD FROM SAVA LAKE (SERBIA). in Slovenian Veterinary Research. 2015;52(2):57-65.
https://hdl.handle.net/21.15107/rcub_ibiss_2060 .

Jacimovic, Milica, Lenhardt, Mirjana, Visnjic-Jeftic, Zeljka, Jaric, Ivan, Gacic, Zoran, Hegedis, Aleksandar, Krpo-Cetkovic, Jasmina, "ELEMENTAL CONCENTRATIONS IN DIFFERENT TISSUES OF EUROPEAN PERCH AND
 BLACK BULLHEAD FROM SAVA LAKE (SERBIA)" in Slovenian Veterinary Research, 52, no. 2 (2015):57-65,
https://hdl.handle.net/21.15107/rcub_ibiss_2060 .

TY  - JOUR
AU  - Vukovic-Gacic, Branka
AU  - Kolarevic, Stoimir
AU  - Sunjog, Karolina
AU  - Tomović, Jelena
AU  - Knežević-Vukčević, Jelena
AU  - Paunović, Momir
AU  - Gacic, Zoran
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2174
AB  - Genotoxic response of freshwater mussels U. tumidus and U. pictorum to
   environmental stress was studied using comet assay on hemocytes and gill
   cells. The mussels were acclimated to controlled laboratory conditions
   for 10 days, and then exposed at 4 sites in the Sava and Danube rivers
   in the area of the city of Belgrade. Samples of each species were taken
   after 7, 14, and 30 days of exposure. The mussels sampled immediately
   after acclimation served as controls. Genotoxic response in both species
   was induced earlier at sites receiving untreated wastewaters from the
   city's main collectors (7 days), than at the site receiving only
   domestic wastewaters from small settlements located upstream from the
   city (30 days). There was a correlation between the comet tail intensity
   values in tissues of exposed mussels and the concentrations of zinc,
   copper, iron, and arsenic at the exposure sites. The genotoxic responses
   in both tissues of U. pictorum and in hemocytes of U. tumidus were
   similar, while U. tumidus gill cells failed to exhibit significant
   genotoxic response at two sites. These findings, together with higher
   mortality of U. tumidus at the most polluted sites, promote U. pictorum
   as a model for genotoxicity monitoring in freshwater environments.
T2  - Hydrobiologia
T1  - Comparative study of the genotoxic response of freshwater mussels Unio
 tumidus and Unio pictorum to environmental stress
IS  - 1
VL  - 735
DO  - 10.1007/s10750-013-1513-x
SP  - 221
EP  - 231
ER  - 

@article{
author = "Vukovic-Gacic, Branka and Kolarevic, Stoimir and Sunjog, Karolina and Tomović, Jelena and Knežević-Vukčević, Jelena and Paunović, Momir and Gacic, Zoran",
year = "2014",
abstract = "Genotoxic response of freshwater mussels U. tumidus and U. pictorum to
   environmental stress was studied using comet assay on hemocytes and gill
   cells. The mussels were acclimated to controlled laboratory conditions
   for 10 days, and then exposed at 4 sites in the Sava and Danube rivers
   in the area of the city of Belgrade. Samples of each species were taken
   after 7, 14, and 30 days of exposure. The mussels sampled immediately
   after acclimation served as controls. Genotoxic response in both species
   was induced earlier at sites receiving untreated wastewaters from the
   city's main collectors (7 days), than at the site receiving only
   domestic wastewaters from small settlements located upstream from the
   city (30 days). There was a correlation between the comet tail intensity
   values in tissues of exposed mussels and the concentrations of zinc,
   copper, iron, and arsenic at the exposure sites. The genotoxic responses
   in both tissues of U. pictorum and in hemocytes of U. tumidus were
   similar, while U. tumidus gill cells failed to exhibit significant
   genotoxic response at two sites. These findings, together with higher
   mortality of U. tumidus at the most polluted sites, promote U. pictorum
   as a model for genotoxicity monitoring in freshwater environments.",
journal = "Hydrobiologia",
title = "Comparative study of the genotoxic response of freshwater mussels Unio
 tumidus and Unio pictorum to environmental stress",
number = "1",
volume = "735",
doi = "10.1007/s10750-013-1513-x",
pages = "221-231"
}

Vukovic-Gacic, B., Kolarevic, S., Sunjog, K., Tomović, J., Knežević-Vukčević, J., Paunović, M.,& Gacic, Z.. (2014). Comparative study of the genotoxic response of freshwater mussels Unio
 tumidus and Unio pictorum to environmental stress. in Hydrobiologia, 735(1), 221-231.
https://doi.org/10.1007/s10750-013-1513-x

Vukovic-Gacic B, Kolarevic S, Sunjog K, Tomović J, Knežević-Vukčević J, Paunović M, Gacic Z. Comparative study of the genotoxic response of freshwater mussels Unio
 tumidus and Unio pictorum to environmental stress. in Hydrobiologia. 2014;735(1):221-231.
doi:10.1007/s10750-013-1513-x .

Vukovic-Gacic, Branka, Kolarevic, Stoimir, Sunjog, Karolina, Tomović, Jelena, Knežević-Vukčević, Jelena, Paunović, Momir, Gacic, Zoran, "Comparative study of the genotoxic response of freshwater mussels Unio
 tumidus and Unio pictorum to environmental stress" in Hydrobiologia, 735, no. 1 (2014):221-231,
https://doi.org/10.1007/s10750-013-1513-x . .

TY  - JOUR
AU  - Tomović, Jelena
AU  - Paunović, Momir
AU  - Atanacković, Ana
AU  - Markovic, Vanja
AU  - Gacic, Zoran
AU  - Csanyi, Bela
AU  - Simic, Vladica
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2109
AB  - The aim of this study is to present the distribution of aquatic molluscs
   along a 2600 km long stretch of the Danube River based on information
   obtained during the Second Joint Danube Survey (JDS2) in 2007. The
   collected data are used to validate the abiotic typology of the Danube
   River and to determine the extent of variations in mollusc richness and
   composition that can be explained by the basic habitat characteristics
   of the sites. The examination of the distribution of mollusc fauna
   reveals differences between the three main sectors of the river, the
   Upper, Middle and Lower Danube. The Iron Gate Section was identified as
   the boundary between the Middle and Lower, Danube. A total of 42 aquatic
   mollusc species-group taxa of 14 families are detected within the study
   area. The Middle and Upper sectors are characterised by higher species
   richness and diversity as compared to the Lower Danube.
T2  - Acta Zoologica Bulgarica
T1  - Biotic Typology of the Danube River Based on Distribution of Mollusc
 Fauna as Revealed by the Second Joint Danube Survey (2007)
IS  - 4
VL  - 66
SP  - 527
EP  - 537
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2109
ER  - 

@article{
author = "Tomović, Jelena and Paunović, Momir and Atanacković, Ana and Markovic, Vanja and Gacic, Zoran and Csanyi, Bela and Simic, Vladica",
year = "2014",
abstract = "The aim of this study is to present the distribution of aquatic molluscs
   along a 2600 km long stretch of the Danube River based on information
   obtained during the Second Joint Danube Survey (JDS2) in 2007. The
   collected data are used to validate the abiotic typology of the Danube
   River and to determine the extent of variations in mollusc richness and
   composition that can be explained by the basic habitat characteristics
   of the sites. The examination of the distribution of mollusc fauna
   reveals differences between the three main sectors of the river, the
   Upper, Middle and Lower Danube. The Iron Gate Section was identified as
   the boundary between the Middle and Lower, Danube. A total of 42 aquatic
   mollusc species-group taxa of 14 families are detected within the study
   area. The Middle and Upper sectors are characterised by higher species
   richness and diversity as compared to the Lower Danube.",
journal = "Acta Zoologica Bulgarica",
title = "Biotic Typology of the Danube River Based on Distribution of Mollusc
 Fauna as Revealed by the Second Joint Danube Survey (2007)",
number = "4",
volume = "66",
pages = "527-537",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2109"
}

Tomović, J., Paunović, M., Atanacković, A., Markovic, V., Gacic, Z., Csanyi, B.,& Simic, V.. (2014). Biotic Typology of the Danube River Based on Distribution of Mollusc
 Fauna as Revealed by the Second Joint Danube Survey (2007). in Acta Zoologica Bulgarica, 66(4), 527-537.
https://hdl.handle.net/21.15107/rcub_ibiss_2109

Tomović J, Paunović M, Atanacković A, Markovic V, Gacic Z, Csanyi B, Simic V. Biotic Typology of the Danube River Based on Distribution of Mollusc
 Fauna as Revealed by the Second Joint Danube Survey (2007). in Acta Zoologica Bulgarica. 2014;66(4):527-537.
https://hdl.handle.net/21.15107/rcub_ibiss_2109 .

Tomović, Jelena, Paunović, Momir, Atanacković, Ana, Markovic, Vanja, Gacic, Zoran, Csanyi, Bela, Simic, Vladica, "Biotic Typology of the Danube River Based on Distribution of Mollusc
 Fauna as Revealed by the Second Joint Danube Survey (2007)" in Acta Zoologica Bulgarica, 66, no. 4 (2014):527-537,
https://hdl.handle.net/21.15107/rcub_ibiss_2109 .

TY  - JOUR
AU  - Heberger, Karoly
AU  - Kolarevic, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Gacic, Zoran
AU  - Kljajic, Zoran
AU  - Mitric, Milena
AU  - Vukovic-Gacic, Branka
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2165
AB  - Specimens of the mussel Mytilus galloprovincialis were collected from
   five sites in the Boka Kotorska Bay (Adriatic Sea, Montenegro) during
   the period summer 2011-autumn 2012. Three types of tissue, haemolymph,
   digestive gland were used for assessment of DNA damage. Images of
   randomly selected cells were analyzed with a fluorescence microscope and
   image analysis by the Comet Assay IV Image-analysis system. Three
   parameters, viz, tail length, tail intensity and Olive tail moment were
   analyzed on 4200 nuclei per cell type. We observed variations in the
   level of DNA damage in mussels collected at different sites, as well as
   seasonal variations in response. Sum of ranking differences (SRD) was
   implemented to compare use of different types of cell and different
   measure of comet tail per nucleus. Numerical scales were transferred
   into ranks, range scaling between 0 and 1; standardization and
   normalization were carried out.
   SRD selected the best (and worst) combinations: tail moment is the best
   for all data treatment and for all organs; second best is tail length,
   and intensity ranks third (except for digestive gland). The differences
   were significant at the 5\% level. Whereas gills and haemolymph cells do
   not differ significantly, cells of the digestive gland are much more
   suitable to estimate genotoxicity. Variance analysis decomposed the
   effect of different factors on the SRD values. This unique combination
   has provided not only the relative importance of factors, but also an
   overall evaluation: the best evaluation method, the best data
   pre-treatment, etc., were chosen even for partially contradictory data.
   The rank transformation is superior to any other way of scaling, which
   is proven by ordering the SRD values by SRD again, and by cross
   validation. (C) 2014 Elsevier B.V. All rights reserved.
T2  - Mutation Research-Genetic Toxicology and Environmental Mutagenesis
T1  - Evaluation of single-cell gel electrophoresis data: Combination of
 variance analysis with sum of ranking differences
VL  - 771
DO  - 10.1016/j.mrgentox.2014.04.028
SP  - 15
EP  - 22
ER  - 

@article{
author = "Heberger, Karoly and Kolarevic, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Gacic, Zoran and Kljajic, Zoran and Mitric, Milena and Vukovic-Gacic, Branka",
year = "2014",
abstract = "Specimens of the mussel Mytilus galloprovincialis were collected from
   five sites in the Boka Kotorska Bay (Adriatic Sea, Montenegro) during
   the period summer 2011-autumn 2012. Three types of tissue, haemolymph,
   digestive gland were used for assessment of DNA damage. Images of
   randomly selected cells were analyzed with a fluorescence microscope and
   image analysis by the Comet Assay IV Image-analysis system. Three
   parameters, viz, tail length, tail intensity and Olive tail moment were
   analyzed on 4200 nuclei per cell type. We observed variations in the
   level of DNA damage in mussels collected at different sites, as well as
   seasonal variations in response. Sum of ranking differences (SRD) was
   implemented to compare use of different types of cell and different
   measure of comet tail per nucleus. Numerical scales were transferred
   into ranks, range scaling between 0 and 1; standardization and
   normalization were carried out.
   SRD selected the best (and worst) combinations: tail moment is the best
   for all data treatment and for all organs; second best is tail length,
   and intensity ranks third (except for digestive gland). The differences
   were significant at the 5\% level. Whereas gills and haemolymph cells do
   not differ significantly, cells of the digestive gland are much more
   suitable to estimate genotoxicity. Variance analysis decomposed the
   effect of different factors on the SRD values. This unique combination
   has provided not only the relative importance of factors, but also an
   overall evaluation: the best evaluation method, the best data
   pre-treatment, etc., were chosen even for partially contradictory data.
   The rank transformation is superior to any other way of scaling, which
   is proven by ordering the SRD values by SRD again, and by cross
   validation. (C) 2014 Elsevier B.V. All rights reserved.",
journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis",
title = "Evaluation of single-cell gel electrophoresis data: Combination of
 variance analysis with sum of ranking differences",
volume = "771",
doi = "10.1016/j.mrgentox.2014.04.028",
pages = "15-22"
}

Heberger, K., Kolarevic, S., Kračun-Kolarević, M., Sunjog, K., Gacic, Z., Kljajic, Z., Mitric, M.,& Vukovic-Gacic, B.. (2014). Evaluation of single-cell gel electrophoresis data: Combination of
 variance analysis with sum of ranking differences. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 771, 15-22.
https://doi.org/10.1016/j.mrgentox.2014.04.028

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Heberger, Karoly, Kolarevic, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Gacic, Zoran, Kljajic, Zoran, Mitric, Milena, Vukovic-Gacic, Branka, "Evaluation of single-cell gel electrophoresis data: Combination of
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