TY  - JOUR
AU  - Dragicević, Ivana C
AU  - Konjević, Radomir M
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
AU  - Nešković, Mirjana
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1540
AB  - Potato (Solanum tuberosum cv. Desiree) shoots grown in vitro in continuous darkness or in long days (LDs), were used to investigate indole-3-acetic acid (IAA) effects on stolon initiation and tuber formation, combining IAA with increased or decreased gibberellin levels. An increased gibberellin (GA) level was achieved by the applying 1 mu M GA(3), while decreased gibberellin level was presumably realized by the adding 3 mu M tetcyclacis (Tc). About 15% of potato shoots developed stolons both in LDs and in darkness. Stolon initiation was stimulated by GA(3) in darkness and by Tc in LDs. Tuber formation was strongly inhibited in LDs and by GA(3) both in light and darkness, but stimulated in darkness at low GA level. Exceptionally, tuber formation occurred in LDs at the highest Tc concentrations, in about 25% of explants. Indole-3-acetic acid alone stimulated stolon formation in LDs, both in the presence or absence of GA(3). IAA alone also stimulated tuber formation in dark-grown shoots, but could not overcome the inhibitory effect of LDs. Indications that, depending on their concentration ratio, IAA may interact with GA(3) in different tuberization phases, have been discussed.
T2  - Plant Growth Regulation
T1  - The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro
IS  - 3
VL  - 54
EP  - 193
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1540
ER  - 

@article{
author = "Dragicević, Ivana C and Konjević, Radomir M and Vinterhalter, Branka and Vinterhalter, Dragan V and Nešković, Mirjana",
year = "2008",
abstract = "Potato (Solanum tuberosum cv. Desiree) shoots grown in vitro in continuous darkness or in long days (LDs), were used to investigate indole-3-acetic acid (IAA) effects on stolon initiation and tuber formation, combining IAA with increased or decreased gibberellin levels. An increased gibberellin (GA) level was achieved by the applying 1 mu M GA(3), while decreased gibberellin level was presumably realized by the adding 3 mu M tetcyclacis (Tc). About 15% of potato shoots developed stolons both in LDs and in darkness. Stolon initiation was stimulated by GA(3) in darkness and by Tc in LDs. Tuber formation was strongly inhibited in LDs and by GA(3) both in light and darkness, but stimulated in darkness at low GA level. Exceptionally, tuber formation occurred in LDs at the highest Tc concentrations, in about 25% of explants. Indole-3-acetic acid alone stimulated stolon formation in LDs, both in the presence or absence of GA(3). IAA alone also stimulated tuber formation in dark-grown shoots, but could not overcome the inhibitory effect of LDs. Indications that, depending on their concentration ratio, IAA may interact with GA(3) in different tuberization phases, have been discussed.",
journal = "Plant Growth Regulation",
title = "The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro",
number = "3",
volume = "54",
pages = "193",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1540"
}

Dragicević, I. C., Konjević, R. M., Vinterhalter, B., Vinterhalter, D. V.,& Nešković, M.. (2008). The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro. in Plant Growth Regulation, 54(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1540

Dragicević IC, Konjević RM, Vinterhalter B, Vinterhalter DV, Nešković M. The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro. in Plant Growth Regulation. 2008;54(3):null-193.
https://hdl.handle.net/21.15107/rcub_ibiss_1540 .

Dragicević, Ivana C, Konjević, Radomir M, Vinterhalter, Branka, Vinterhalter, Dragan V, Nešković, Mirjana, "The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures in vitro" in Plant Growth Regulation, 54, no. 3 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1540 .

TY  - JOUR
AU  - Skorić, Marijana
AU  - Momčilović, Ivana
AU  - Krstić, Dijana
AU  - Maksimović, Vuk M
AU  - Konjević, Radomir M
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1672
AB  - A protocol for in vitro propagation of Gentiana asclepiadea was developed. The best multiplication rate was obtained with woody plant medium (WPM) supplemented with 8.9 mu M BAP (6-benzylaminopurine) and 1.1 mu M IAA (indole-3-acetic acid). Gibberellic acid in the presence of 8.9 mu M BAP and 1.1 mu M IAA stimulated shoot elongation without affecting multiplication index. Although spontaneous rooting on hormone-free medium was observed, auxins increased the rooting ability. Treatment with IBA (indole-3-butyric acid) induced a higher number of roots, while the addition of IAA caused an increase in root length. The accumulation of mangiferin and gentiopicrine, in G. asclepiadea grown in vitro, was much lower in roots than in shoots. The accumulation of gentiopicrine and mangiferin in cultured plants was significantly enhanced in the presence of cytokinins (BAP), even above the level of plants from natural habitat.
T2  - Phyton-Annales Rei Botanicae
T1  - In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin
IS  - 1
VL  - 46
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1672
ER  - 

@article{
author = "Skorić, Marijana and Momčilović, Ivana and Krstić, Dijana and Maksimović, Vuk M and Konjević, Radomir M",
year = "2006",
abstract = "A protocol for in vitro propagation of Gentiana asclepiadea was developed. The best multiplication rate was obtained with woody plant medium (WPM) supplemented with 8.9 mu M BAP (6-benzylaminopurine) and 1.1 mu M IAA (indole-3-acetic acid). Gibberellic acid in the presence of 8.9 mu M BAP and 1.1 mu M IAA stimulated shoot elongation without affecting multiplication index. Although spontaneous rooting on hormone-free medium was observed, auxins increased the rooting ability. Treatment with IBA (indole-3-butyric acid) induced a higher number of roots, while the addition of IAA caused an increase in root length. The accumulation of mangiferin and gentiopicrine, in G. asclepiadea grown in vitro, was much lower in roots than in shoots. The accumulation of gentiopicrine and mangiferin in cultured plants was significantly enhanced in the presence of cytokinins (BAP), even above the level of plants from natural habitat.",
journal = "Phyton-Annales Rei Botanicae",
title = "In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin",
number = "1",
volume = "46",
pages = "54",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1672"
}

Skorić, M., Momčilović, I., Krstić, D., Maksimović, V. M.,& Konjević, R. M.. (2006). In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin. in Phyton-Annales Rei Botanicae, 46(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1672

Skorić M, Momčilović I, Krstić D, Maksimović VM, Konjević RM. In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin. in Phyton-Annales Rei Botanicae. 2006;46(1):null-54.
https://hdl.handle.net/21.15107/rcub_ibiss_1672 .

Skorić, Marijana, Momčilović, Ivana, Krstić, Dijana, Maksimović, Vuk M, Konjević, Radomir M, "In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin" in Phyton-Annales Rei Botanicae, 46, no. 1 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1672 .

TY  - JOUR
AU  - Todorović, Slađana
AU  - Grubišić, Dragoljub
AU  - Giba, Zlatko
AU  - Mišić, Danijela
AU  - Konjević, Radomir M
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1626
AB  - The effect of sucrose on fruiting, seed production, and seed germination of lesser centaury [Centaurium pulchellum (Sw.) Druce] was examined using explants of flowers and flower buds. Sucrose concentrations in the culture medium ranged from 0.003 to 0.3 M. It has been shown that the number of auxiliary buds, capsules dimension, number of viable seeds per capsule and seed dimensions increased with the increase of sucrose concentrations. The highest values were recorded at sucrose concentrations higher than 0.03 M, except for seeds size, which were larger at sucrose concentration ranging from 0.003 to 0.1 M. The germination of in vitro produced seeds was affected by previous culture history: a higher germination percentage was obtained in seeds that were raised from explants originally grown on medium with sucrose concentrations higher than 0.003 M.
T2  - Biologia Plantarum
T1  - Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum
IS  - 4
VL  - 50
EP  - 774
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1626
ER  - 

@article{
author = "Todorović, Slađana and Grubišić, Dragoljub and Giba, Zlatko and Mišić, Danijela and Konjević, Radomir M",
year = "2006",
abstract = "The effect of sucrose on fruiting, seed production, and seed germination of lesser centaury [Centaurium pulchellum (Sw.) Druce] was examined using explants of flowers and flower buds. Sucrose concentrations in the culture medium ranged from 0.003 to 0.3 M. It has been shown that the number of auxiliary buds, capsules dimension, number of viable seeds per capsule and seed dimensions increased with the increase of sucrose concentrations. The highest values were recorded at sucrose concentrations higher than 0.03 M, except for seeds size, which were larger at sucrose concentration ranging from 0.003 to 0.1 M. The germination of in vitro produced seeds was affected by previous culture history: a higher germination percentage was obtained in seeds that were raised from explants originally grown on medium with sucrose concentrations higher than 0.003 M.",
journal = "Biologia Plantarum",
title = "Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum",
number = "4",
volume = "50",
pages = "774",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1626"
}

Todorović, S., Grubišić, D., Giba, Z., Mišić, D.,& Konjević, R. M.. (2006). Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum. in Biologia Plantarum, 50(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1626

Todorović S, Grubišić D, Giba Z, Mišić D, Konjević RM. Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum. in Biologia Plantarum. 2006;50(4):null-774.
https://hdl.handle.net/21.15107/rcub_ibiss_1626 .

Todorović, Slađana, Grubišić, Dragoljub, Giba, Zlatko, Mišić, Danijela, Konjević, Radomir M, "Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum" in Biologia Plantarum, 50, no. 4 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1626 .

TY  - JOUR
AU  - Todorović, Slađana
AU  - Giba, Zlatko
AU  - Živković, Suzana
AU  - Grubišić, Dragoljub
AU  - Konjević, Radomir M
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1688
AB  - The germination of Empress tree (Paulownia tomentosa Steud.) seeds is phytochrome-controlled. Liquid smoke could not induce germination in darkness but red light irradiation of liquid smoke imbibed seeds induced a high percentage of germination. Maximum germination was achieved at liquid smoke concentration of 0.1% (v/v) when present during the imbibition phase or during the phase of phytochrome activity. The light requirement of these seeds could be completely substituted by exogenously applied gibberellins. In the presence of liquid smoke, optimal concentrations of GA(3), GA(4), and GA(9) necessary for inducing germination were several times lower than in the controls, while that of GA(7) was equally active when applied at a concentration one order of magnitude lower. The inhibitory effect of the applied growth retardants was strongly reduced and liquid smoke, in the presence of retardants, allowed light-induced germination, if applied simultaneously or after retardants treatment.
T2  - Plant Growth Regulation
T1  - Stimulation of empress tree seed germination by liquid smoke
IS  - 2-3
VL  - 47
EP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1688
ER  - 

@article{
author = "Todorović, Slađana and Giba, Zlatko and Živković, Suzana and Grubišić, Dragoljub and Konjević, Radomir M",
year = "2005",
abstract = "The germination of Empress tree (Paulownia tomentosa Steud.) seeds is phytochrome-controlled. Liquid smoke could not induce germination in darkness but red light irradiation of liquid smoke imbibed seeds induced a high percentage of germination. Maximum germination was achieved at liquid smoke concentration of 0.1% (v/v) when present during the imbibition phase or during the phase of phytochrome activity. The light requirement of these seeds could be completely substituted by exogenously applied gibberellins. In the presence of liquid smoke, optimal concentrations of GA(3), GA(4), and GA(9) necessary for inducing germination were several times lower than in the controls, while that of GA(7) was equally active when applied at a concentration one order of magnitude lower. The inhibitory effect of the applied growth retardants was strongly reduced and liquid smoke, in the presence of retardants, allowed light-induced germination, if applied simultaneously or after retardants treatment.",
journal = "Plant Growth Regulation",
title = "Stimulation of empress tree seed germination by liquid smoke",
number = "2-3",
volume = "47",
pages = "148",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1688"
}

Todorović, S., Giba, Z., Živković, S., Grubišić, D.,& Konjević, R. M.. (2005). Stimulation of empress tree seed germination by liquid smoke. in Plant Growth Regulation, 47(2-3).
https://hdl.handle.net/21.15107/rcub_ibiss_1688

Todorović S, Giba Z, Živković S, Grubišić D, Konjević RM. Stimulation of empress tree seed germination by liquid smoke. in Plant Growth Regulation. 2005;47(2-3):null-148.
https://hdl.handle.net/21.15107/rcub_ibiss_1688 .

Todorović, Slađana, Giba, Zlatko, Živković, Suzana, Grubišić, Dragoljub, Konjević, Radomir M, "Stimulation of empress tree seed germination by liquid smoke" in Plant Growth Regulation, 47, no. 2-3 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1688 .

TY  - CONF
AU  - Jovanović, Vladimir
AU  - Giba, Zlatko
AU  - Đoković, Dejan D
AU  - Milosavljević, Slobodan M
AU  - Grubišić, Dragoljub
AU  - Konjević, Radomir M
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1716
AB  - We used two species of light-requiring seeds, Paulownia tomentosa, which have absolute light requirement (no germination in darkness), and Stellaria media seeds, which germinate in darkness to a certain extent because of presence of preformed active phytochrome, to obtain results strongly suggesting that gibberellic acid nitrite stimulates seed germination via its capability as a functional NO donor. Exogenous application of gibberellic acid nitrite stimulates gibberellin-insensitive Stellaria media seed germination in darkness as do a wide variety of NO donors. Pure gibberellic acid could replace the light requirement of P tomentosa seeds, thus enabling them to germinate in darkness. Gibberellic acid nitrite did not have this effect. A stimulative effect from gibberellic acid nitrite could be detected only after exposure of these seeds to short, 10 min, pulse of red light. Taken together, these results suggest that gibberellic activity of gibberellic acid nitrite is lost after nitrosation but, regarding to the presence of -O-NO moiety in the molecule, gibberellic acid nitrite shares stimulative properties in seed germination with other compounds with NO-releasing properties.
C3  - Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus
T1  - Gibberellic acid nitrite stimulates germination of two species of light-requiring seeds via the nitric oxide pathway
IS  - null
VL  - 1048
EP  - 481
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1716
ER  - 

@conference{
author = "Jovanović, Vladimir and Giba, Zlatko and Đoković, Dejan D and Milosavljević, Slobodan M and Grubišić, Dragoljub and Konjević, Radomir M",
year = "2005",
abstract = "We used two species of light-requiring seeds, Paulownia tomentosa, which have absolute light requirement (no germination in darkness), and Stellaria media seeds, which germinate in darkness to a certain extent because of presence of preformed active phytochrome, to obtain results strongly suggesting that gibberellic acid nitrite stimulates seed germination via its capability as a functional NO donor. Exogenous application of gibberellic acid nitrite stimulates gibberellin-insensitive Stellaria media seed germination in darkness as do a wide variety of NO donors. Pure gibberellic acid could replace the light requirement of P tomentosa seeds, thus enabling them to germinate in darkness. Gibberellic acid nitrite did not have this effect. A stimulative effect from gibberellic acid nitrite could be detected only after exposure of these seeds to short, 10 min, pulse of red light. Taken together, these results suggest that gibberellic activity of gibberellic acid nitrite is lost after nitrosation but, regarding to the presence of -O-NO moiety in the molecule, gibberellic acid nitrite shares stimulative properties in seed germination with other compounds with NO-releasing properties.",
journal = "Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "Gibberellic acid nitrite stimulates germination of two species of light-requiring seeds via the nitric oxide pathway",
number = "null",
volume = "1048",
pages = "481",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1716"
}

Jovanović, V., Giba, Z., Đoković, D. D., Milosavljević, S. M., Grubišić, D.,& Konjević, R. M.. (2005). Gibberellic acid nitrite stimulates germination of two species of light-requiring seeds via the nitric oxide pathway. in Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus, 1048(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1716

Jovanović V, Giba Z, Đoković DD, Milosavljević SM, Grubišić D, Konjević RM. Gibberellic acid nitrite stimulates germination of two species of light-requiring seeds via the nitric oxide pathway. in Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048(null):null-481.
https://hdl.handle.net/21.15107/rcub_ibiss_1716 .

Jovanović, Vladimir, Giba, Zlatko, Đoković, Dejan D, Milosavljević, Slobodan M, Grubišić, Dragoljub, Konjević, Radomir M, "Gibberellic acid nitrite stimulates germination of two species of light-requiring seeds via the nitric oxide pathway" in Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus, 1048, no. null (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1716 .

TY  - JOUR
AU  - Mišić, Danijela
AU  - Maksimović, Vuk M
AU  - Todorović, Slađana
AU  - Grubišić, Dragoljub V.
AU  - Konjević, Radomir M
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1719
AB  - A wide range of sucrose, fructose, and glucose levels in culture media was tested in vitro in order to determine those that provide optimum growth, morphogenesis, and the production of secondary metabolites in Nepeta rtanjensis Diklic and Milojevic shoots. The effect of different concentrations of the carbohydrates in culture media on the internal carbohydrate status of N. rtanjensis shoots was also determined. Our results show that in vitro growth and development of N. rtanjensis, as well as nepetalactone accumulation, are significantly affected by both the type of carbohydrate and its concentration in the culture medium. Glucose proved to be the most efficient carbon and energy source.
T2  - Israel Journal of Plant Sciences
T1  - Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro
IS  - 2
VL  - 53
EP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1719
ER  - 

@article{
author = "Mišić, Danijela and Maksimović, Vuk M and Todorović, Slađana and Grubišić, Dragoljub V. and Konjević, Radomir M",
year = "2005",
abstract = "A wide range of sucrose, fructose, and glucose levels in culture media was tested in vitro in order to determine those that provide optimum growth, morphogenesis, and the production of secondary metabolites in Nepeta rtanjensis Diklic and Milojevic shoots. The effect of different concentrations of the carbohydrates in culture media on the internal carbohydrate status of N. rtanjensis shoots was also determined. Our results show that in vitro growth and development of N. rtanjensis, as well as nepetalactone accumulation, are significantly affected by both the type of carbohydrate and its concentration in the culture medium. Glucose proved to be the most efficient carbon and energy source.",
journal = "Israel Journal of Plant Sciences",
title = "Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro",
number = "2",
volume = "53",
pages = "108",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1719"
}

Mišić, D., Maksimović, V. M., Todorović, S., Grubišić, D. V.,& Konjević, R. M.. (2005). Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro. in Israel Journal of Plant Sciences, 53(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1719

Mišić D, Maksimović VM, Todorović S, Grubišić DV, Konjević RM. Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro. in Israel Journal of Plant Sciences. 2005;53(2):null-108.
https://hdl.handle.net/21.15107/rcub_ibiss_1719 .

Mišić, Danijela, Maksimović, Vuk M, Todorović, Slađana, Grubišić, Dragoljub V., Konjević, Radomir M, "Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro" in Israel Journal of Plant Sciences, 53, no. 2 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1719 .

TY  - JOUR
AU  - Mišić, Danijela
AU  - Ghalawenji, Nabil
AU  - Grubišić, Dragoljub V.
AU  - Konjević, Radomir M
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1722
AB  - A micropropagation protocol was developed for the conservation of critically endangered Serbian perennial Nepeta rtanjensis (Lamiaceae). Rooted shoots were obtained from one-node stem segments and shoot tips on a half-strength Murashige and Skoog (MS) medium without growth regulators. The best pH of the medium for axillary buds induction and for rooting of shoots was found to be at 7 and/or 7.2 respectively. The addition of cytokinins to the culture medium did not significantly stimulated auxillary bud production as compared to the control. On the contrary, on media supplemented with high cytokinin concentrations, only dwarf shoots with rudimentary roots were obtained. All tested concentrations of 6-benzylaminopyrine (BAP) and kinetine (Kn) in combination with 0.1 mg 1(-1) indole-3-acetic acid (IAA) negatively affected the elongation and rooting of shoots. Plants micropropagated on hormone free medium and rooted in vitro were successfully acclimatized in greenhouse and in open field conditions. The result of successful acclimatization was the production of more than 7000 plantlets with normal sexually reproduction. They flowered, fruited and produced seeds which exhibited 47% germination. The survival rate of plants that were transferred to the open field for the acclimatization and exposed to the winter chill was 99%. The reintroduction of N. rtanjensis occurred in May 2004. One thousand plantlets were planted within the historic range of this plant species. The survival rate was also 99%.
T2  - Phyton-Annales Rei Botanicae
T1  - Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia
IS  - 1
VL  - 45
EP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1722
ER  - 

@article{
author = "Mišić, Danijela and Ghalawenji, Nabil and Grubišić, Dragoljub V. and Konjević, Radomir M",
year = "2005",
abstract = "A micropropagation protocol was developed for the conservation of critically endangered Serbian perennial Nepeta rtanjensis (Lamiaceae). Rooted shoots were obtained from one-node stem segments and shoot tips on a half-strength Murashige and Skoog (MS) medium without growth regulators. The best pH of the medium for axillary buds induction and for rooting of shoots was found to be at 7 and/or 7.2 respectively. The addition of cytokinins to the culture medium did not significantly stimulated auxillary bud production as compared to the control. On the contrary, on media supplemented with high cytokinin concentrations, only dwarf shoots with rudimentary roots were obtained. All tested concentrations of 6-benzylaminopyrine (BAP) and kinetine (Kn) in combination with 0.1 mg 1(-1) indole-3-acetic acid (IAA) negatively affected the elongation and rooting of shoots. Plants micropropagated on hormone free medium and rooted in vitro were successfully acclimatized in greenhouse and in open field conditions. The result of successful acclimatization was the production of more than 7000 plantlets with normal sexually reproduction. They flowered, fruited and produced seeds which exhibited 47% germination. The survival rate of plants that were transferred to the open field for the acclimatization and exposed to the winter chill was 99%. The reintroduction of N. rtanjensis occurred in May 2004. One thousand plantlets were planted within the historic range of this plant species. The survival rate was also 99%.",
journal = "Phyton-Annales Rei Botanicae",
title = "Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia",
number = "1",
volume = "45",
pages = "20",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1722"
}

Mišić, D., Ghalawenji, N., Grubišić, D. V.,& Konjević, R. M.. (2005). Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia. in Phyton-Annales Rei Botanicae, 45(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1722

Mišić D, Ghalawenji N, Grubišić DV, Konjević RM. Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia. in Phyton-Annales Rei Botanicae. 2005;45(1):null-20.
https://hdl.handle.net/21.15107/rcub_ibiss_1722 .

Mišić, Danijela, Ghalawenji, Nabil, Grubišić, Dragoljub V., Konjević, Radomir M, "Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia" in Phyton-Annales Rei Botanicae, 45, no. 1 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1722 .

TY  - CONF
AU  - Sabovljević, Aneta
AU  - Grubišić, Dragoljub
AU  - Sabovljević, Marko S
AU  - Konjević, Radomir M
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1744
C3  - Acta Physiologiae Plantarum
T1  - Fructose Induces Maleness in the Moss Bryum Argenteum (Hedw.) Grown in Vitro
IS  - 3
VL  - 26
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1744
ER  - 

@conference{
author = "Sabovljević, Aneta and Grubišić, Dragoljub and Sabovljević, Marko S and Konjević, Radomir M",
year = "2004",
journal = "Acta Physiologiae Plantarum",
title = "Fructose Induces Maleness in the Moss Bryum Argenteum (Hedw.) Grown in Vitro",
number = "3",
volume = "26",
pages = "53",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1744"
}

Sabovljević, A., Grubišić, D., Sabovljević, M. S.,& Konjević, R. M.. (2004). Fructose Induces Maleness in the Moss Bryum Argenteum (Hedw.) Grown in Vitro. in Acta Physiologiae Plantarum, 26(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1744

Sabovljević A, Grubišić D, Sabovljević MS, Konjević RM. Fructose Induces Maleness in the Moss Bryum Argenteum (Hedw.) Grown in Vitro. in Acta Physiologiae Plantarum. 2004;26(3):null-53.
https://hdl.handle.net/21.15107/rcub_ibiss_1744 .

Sabovljević, Aneta, Grubišić, Dragoljub, Sabovljević, Marko S, Konjević, Radomir M, "Fructose Induces Maleness in the Moss Bryum Argenteum (Hedw.) Grown in Vitro" in Acta Physiologiae Plantarum, 26, no. 3 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1744 .

TY  - JOUR
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
AU  - Konjević, Radomir M
PY  - 2003
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1763
AB  - Nitrates have long been known to promote seed germination in many species, and various proposals have been made regarding the role of nitrates in this process. One hypothesis suggests a possible ecological role for nitrate, providing the seeds with a report of soil nitrogen status and the proximity of already established plants. However, nitrogen oxides (NO, NO2), which are present in the soil, also may be information carriers that indicate not only soil nitrate content, but also microbial activity and, therefore, soil quality. Because of annual variation of soil trace gas fluxes, seeds could be provided with information on seasonal and climate changes in their surroundings. Thus, nitrogen oxides would be the outer information carriers providing the seeds with integral data about many important factors required for successful germination and seedling establishment.
T2  - Seed Science Research
T1  - Nitrogen oxides as environmental sensors for seeds
IS  - 3
VL  - 13
EP  - 196
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1763
ER  - 

@article{
author = "Giba, Zlatko and Grubišić, Dragoljub and Konjević, Radomir M",
year = "2003",
abstract = "Nitrates have long been known to promote seed germination in many species, and various proposals have been made regarding the role of nitrates in this process. One hypothesis suggests a possible ecological role for nitrate, providing the seeds with a report of soil nitrogen status and the proximity of already established plants. However, nitrogen oxides (NO, NO2), which are present in the soil, also may be information carriers that indicate not only soil nitrate content, but also microbial activity and, therefore, soil quality. Because of annual variation of soil trace gas fluxes, seeds could be provided with information on seasonal and climate changes in their surroundings. Thus, nitrogen oxides would be the outer information carriers providing the seeds with integral data about many important factors required for successful germination and seedling establishment.",
journal = "Seed Science Research",
title = "Nitrogen oxides as environmental sensors for seeds",
number = "3",
volume = "13",
pages = "196",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1763"
}

Giba, Z., Grubišić, D.,& Konjević, R. M.. (2003). Nitrogen oxides as environmental sensors for seeds. in Seed Science Research, 13(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1763

Giba Z, Grubišić D, Konjević RM. Nitrogen oxides as environmental sensors for seeds. in Seed Science Research. 2003;13(3):null-196.
https://hdl.handle.net/21.15107/rcub_ibiss_1763 .

Giba, Zlatko, Grubišić, Dragoljub, Konjević, Radomir M, "Nitrogen oxides as environmental sensors for seeds" in Seed Science Research, 13, no. 3 (2003),
https://hdl.handle.net/21.15107/rcub_ibiss_1763 .

TY  - JOUR
AU  - Batak, Ivan
AU  - Skorić, Marijana
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
AU  - Poff, KL
AU  - Konjević, Radomir M
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1785
AB  - Nitrogenous compounds, such as potassium nitrate, potentiate germination of different species of light-requiring seeds. Using light-induced Arabidopsis thaliana seed germination as a model system, our data suggested that only phytochrome A (phyA)-specific induced germination was affected after the exogenous application of nitrates, different nitric oxide (NO)-donors (such as organic nitrates) or sodium nitroprusside. The stimulative effect was very pronounced. Treated seed samples reached maximal germination after very short periods of red-light irradiation. To a far lesser extent, these substances affected phytochrome B (phyB)-specific induced germination. In phyB-specific induced germination, potassium nitrate was most effective, but germination percentages never exceeded 50%. The least effective was sodium nitroprusside, which practically did not affect phyB-specific induced germination. These results were confirmed using corresponding phytochrome mutants.
T2  - Seed Science Research
T1  - The effects of potassium nitrate and NO-donors on phytochrome A- and phytochrome B-specific induced germination of Arabidopsis thaliana seeds
IS  - 4
VL  - 12
EP  - 259
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1785
ER  - 

@article{
author = "Batak, Ivan and Skorić, Marijana and Giba, Zlatko and Grubišić, Dragoljub and Poff, KL and Konjević, Radomir M",
year = "2002",
abstract = "Nitrogenous compounds, such as potassium nitrate, potentiate germination of different species of light-requiring seeds. Using light-induced Arabidopsis thaliana seed germination as a model system, our data suggested that only phytochrome A (phyA)-specific induced germination was affected after the exogenous application of nitrates, different nitric oxide (NO)-donors (such as organic nitrates) or sodium nitroprusside. The stimulative effect was very pronounced. Treated seed samples reached maximal germination after very short periods of red-light irradiation. To a far lesser extent, these substances affected phytochrome B (phyB)-specific induced germination. In phyB-specific induced germination, potassium nitrate was most effective, but germination percentages never exceeded 50%. The least effective was sodium nitroprusside, which practically did not affect phyB-specific induced germination. These results were confirmed using corresponding phytochrome mutants.",
journal = "Seed Science Research",
title = "The effects of potassium nitrate and NO-donors on phytochrome A- and phytochrome B-specific induced germination of Arabidopsis thaliana seeds",
number = "4",
volume = "12",
pages = "259",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1785"
}

Batak, I., Skorić, M., Giba, Z., Grubišić, D., Poff, K.,& Konjević, R. M.. (2002). The effects of potassium nitrate and NO-donors on phytochrome A- and phytochrome B-specific induced germination of Arabidopsis thaliana seeds. in Seed Science Research, 12(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1785

Batak I, Skorić M, Giba Z, Grubišić D, Poff K, Konjević RM. The effects of potassium nitrate and NO-donors on phytochrome A- and phytochrome B-specific induced germination of Arabidopsis thaliana seeds. in Seed Science Research. 2002;12(4):null-259.
https://hdl.handle.net/21.15107/rcub_ibiss_1785 .

Batak, Ivan, Skorić, Marijana, Giba, Zlatko, Grubišić, Dragoljub, Poff, KL, Konjević, Radomir M, "The effects of potassium nitrate and NO-donors on phytochrome A- and phytochrome B-specific induced germination of Arabidopsis thaliana seeds" in Seed Science Research, 12, no. 4 (2002),
https://hdl.handle.net/21.15107/rcub_ibiss_1785 .

TY  - JOUR
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
AU  - Todorović, S
AU  - Sajc, L
AU  - Stojaković, D
AU  - Konjević, Radomir M
PY  - 1998
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1852
AB  - Using different nitric oxide releasing compounds and appropriate controls we have obtained data strongly suggesting the involvement of nitric oxide in the phytochrome controlled germination of Paulownia tomentosa seeds. Direct detection of nitric oxide, under various experimental conditions, was performed by a spin-trapping technique combined with electron paramagnetic resonance (EPR) spectroscopy. The addition of methylene blue prevented light-induced and NO donors-potentiated germination of P. tomentosa seeds. This inhibition could be completely overcome by addition of gibberellin. The promotive effect of nitrite was pH dependent, maximally pronounced at the pH range where nitrite undergoes dismutation and liberates nitric oxide. Under these conditions, nitrite exerted its efficacy at the same concentrations at which nitric oxide releasing compounds such as sodium nitroprusside (SNP), S-nitroso acetylpenicillamine (SNAP), and 3-morpholinosydnonimine (SIN-1), were the most effective. Likewise, the potentiation of P. tomentosa seed germination could be achieved by chemical reduction of nitrite with Na2S2O4 during which liberation of nitric oxide could be detected.
T2  - Plant Growth Regulation
T1  - Effect of nitric oxide - releasing compounds on phytochrome - controlled germination of Empress tree seeds
IS  - 3
VL  - 26
EP  - 181
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1852
ER  - 

@article{
author = "Giba, Zlatko and Grubišić, Dragoljub and Todorović, S and Sajc, L and Stojaković, D and Konjević, Radomir M",
year = "1998",
abstract = "Using different nitric oxide releasing compounds and appropriate controls we have obtained data strongly suggesting the involvement of nitric oxide in the phytochrome controlled germination of Paulownia tomentosa seeds. Direct detection of nitric oxide, under various experimental conditions, was performed by a spin-trapping technique combined with electron paramagnetic resonance (EPR) spectroscopy. The addition of methylene blue prevented light-induced and NO donors-potentiated germination of P. tomentosa seeds. This inhibition could be completely overcome by addition of gibberellin. The promotive effect of nitrite was pH dependent, maximally pronounced at the pH range where nitrite undergoes dismutation and liberates nitric oxide. Under these conditions, nitrite exerted its efficacy at the same concentrations at which nitric oxide releasing compounds such as sodium nitroprusside (SNP), S-nitroso acetylpenicillamine (SNAP), and 3-morpholinosydnonimine (SIN-1), were the most effective. Likewise, the potentiation of P. tomentosa seed germination could be achieved by chemical reduction of nitrite with Na2S2O4 during which liberation of nitric oxide could be detected.",
journal = "Plant Growth Regulation",
title = "Effect of nitric oxide - releasing compounds on phytochrome - controlled germination of Empress tree seeds",
number = "3",
volume = "26",
pages = "181",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1852"
}

Giba, Z., Grubišić, D., Todorović, S., Sajc, L., Stojaković, D.,& Konjević, R. M.. (1998). Effect of nitric oxide - releasing compounds on phytochrome - controlled germination of Empress tree seeds. in Plant Growth Regulation, 26(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1852

Giba Z, Grubišić D, Todorović S, Sajc L, Stojaković D, Konjević RM. Effect of nitric oxide - releasing compounds on phytochrome - controlled germination of Empress tree seeds. in Plant Growth Regulation. 1998;26(3):null-181.
https://hdl.handle.net/21.15107/rcub_ibiss_1852 .

Giba, Zlatko, Grubišić, Dragoljub, Todorović, S, Sajc, L, Stojaković, D, Konjević, Radomir M, "Effect of nitric oxide - releasing compounds on phytochrome - controlled germination of Empress tree seeds" in Plant Growth Regulation, 26, no. 3 (1998),
https://hdl.handle.net/21.15107/rcub_ibiss_1852 .

TY  - JOUR
AU  - Janoudi, AK
AU  - Konjević, Radomir M
AU  - Whitelam, G
AU  - Gordon, W
AU  - Poff, KL
PY  - 1997
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1872
AB  - The role of phytochrome A (phyA) and phytochrome B (phyB) in phototropism was investigated by using the phytochrome-deficient mutants phyA-101, phyB-1 and a phyA/phyB double mutant. The red-light-induced enhancement of phototropism, which is normally observed in wild-type seedlings, could not be detected in the phyA/phyB mutant at fluences of red light between 0.1 and 19 000 mu mol m(-2). The loss of phyB has been shown to have no apparent effect on enhancement, while the loss of phyA resulted in a loss of enhancement only in the low fluence range (Janoudi et al. 1997). The conclusions of the aforementioned study can now be modified based on the current results which indicate that phototropic enhancement in the high fluence range is mediated by either phyA or phyB, and that other phytochromes have no role in enhancement. First positive phototropism was unaffected in phyA-101 and phyB-1. However, the magnitude of first positive phototropism in the phyA/phyB mutant was significantly lower than that of the wild-type Landsberg parent. Thus, the presence of either phyA or phyB is required for normal expression of first positive phototropism. The time threshold for second positive phototropism is unaltered in the phyA-101 and phyB-1 mutants. However, the time threshold in the phyA/phyB mutant is about 2 h, approximately six times that of the wild type. Finally, the magnitude of second positive phototropism in both phyA-101 and phyB-1 is diminished in comparison with the wild-type response. Thus, phyA and phyB, acting independently or in combination, regulate the magnitude of phototropic curvature and the time threshold for second positive phototropism. We conclude that the presence of phyA and phyB is required, but not sufficient, for the expression of normal phototropism.
T2  - Physiologia Plantarum
T1  - Both phytochrome A and phytochrome B are required for the normal expression of phototropism in Arabidopsis thaliana seedlings
IS  - 2
VL  - 101
EP  - 282
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1872
ER  - 

@article{
author = "Janoudi, AK and Konjević, Radomir M and Whitelam, G and Gordon, W and Poff, KL",
year = "1997",
abstract = "The role of phytochrome A (phyA) and phytochrome B (phyB) in phototropism was investigated by using the phytochrome-deficient mutants phyA-101, phyB-1 and a phyA/phyB double mutant. The red-light-induced enhancement of phototropism, which is normally observed in wild-type seedlings, could not be detected in the phyA/phyB mutant at fluences of red light between 0.1 and 19 000 mu mol m(-2). The loss of phyB has been shown to have no apparent effect on enhancement, while the loss of phyA resulted in a loss of enhancement only in the low fluence range (Janoudi et al. 1997). The conclusions of the aforementioned study can now be modified based on the current results which indicate that phototropic enhancement in the high fluence range is mediated by either phyA or phyB, and that other phytochromes have no role in enhancement. First positive phototropism was unaffected in phyA-101 and phyB-1. However, the magnitude of first positive phototropism in the phyA/phyB mutant was significantly lower than that of the wild-type Landsberg parent. Thus, the presence of either phyA or phyB is required for normal expression of first positive phototropism. The time threshold for second positive phototropism is unaltered in the phyA-101 and phyB-1 mutants. However, the time threshold in the phyA/phyB mutant is about 2 h, approximately six times that of the wild type. Finally, the magnitude of second positive phototropism in both phyA-101 and phyB-1 is diminished in comparison with the wild-type response. Thus, phyA and phyB, acting independently or in combination, regulate the magnitude of phototropic curvature and the time threshold for second positive phototropism. We conclude that the presence of phyA and phyB is required, but not sufficient, for the expression of normal phototropism.",
journal = "Physiologia Plantarum",
title = "Both phytochrome A and phytochrome B are required for the normal expression of phototropism in Arabidopsis thaliana seedlings",
number = "2",
volume = "101",
pages = "282",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1872"
}

Janoudi, A., Konjević, R. M., Whitelam, G., Gordon, W.,& Poff, K.. (1997). Both phytochrome A and phytochrome B are required for the normal expression of phototropism in Arabidopsis thaliana seedlings. in Physiologia Plantarum, 101(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1872

Janoudi A, Konjević RM, Whitelam G, Gordon W, Poff K. Both phytochrome A and phytochrome B are required for the normal expression of phototropism in Arabidopsis thaliana seedlings. in Physiologia Plantarum. 1997;101(2):null-282.
https://hdl.handle.net/21.15107/rcub_ibiss_1872 .

Janoudi, AK, Konjević, Radomir M, Whitelam, G, Gordon, W, Poff, KL, "Both phytochrome A and phytochrome B are required for the normal expression of phototropism in Arabidopsis thaliana seedlings" in Physiologia Plantarum, 101, no. 2 (1997),
https://hdl.handle.net/21.15107/rcub_ibiss_1872 .