TY  - JOUR
AU  - Oalđe Pavlović, Mariana
AU  - Lunić, Tanja
AU  - Graovac, Stefan
AU  - Mandić, Marija
AU  - Repac, Jelena
AU  - Gašić, Uroš
AU  - Božić Nedeljković, Biljana
AU  - Božić, Bojan
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669022006835
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5013
AB  - Diabetes mellitus has been recognized as one of the most challenging public health problems in the 21st century. The present study aimed to investigate the antidiabetic potential of 18 selected Lamiaceae representatives on yeast α-glucosidase and further translate experimental data into predicted human α-glucosidase inhibition. Since conventional antidiabetic drugs exhibit numerous unavoidable side effects, plant extracts are being increasingly explored as safer alternatives. Plant extracts were prepared by maceration, characterized by liquid chromatography-mass spectrometry (LC-MS), and subjected to in vitro determination of yeast α-glucosidase inhibitory potential, after which the kinetic study of one of the most potent extracts (Mentha × piperita) was performed. Docking simulations were done on both yeast and human α-glucosidase to explore binding modes and energies of the secondary metabolites identified in the examined extracts. Finally, the dynamical model has been constructed (adjR2 = 0.9885) and applied to explore extracts’ inhibition potential onto the human α-glucosidase. The constructed dynamical model revealed, for the first time, that different extracts of Satureja montana represent highly promising candidates for the prevention/treatment of diabetes mellitus. Therefore, the dynamical model described in this study represents a useful tool and a significant advance in the discovery of novel therapeutics with antidiabetic potential in humans.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling
VL  - 186
DO  - 10.1016/j.indcrop.2022.115200
SP  - 115200
ER  - 

@article{
author = "Oalđe Pavlović, Mariana and Lunić, Tanja and Graovac, Stefan and Mandić, Marija and Repac, Jelena and Gašić, Uroš and Božić Nedeljković, Biljana and Božić, Bojan",
year = "2022",
abstract = "Diabetes mellitus has been recognized as one of the most challenging public health problems in the 21st century. The present study aimed to investigate the antidiabetic potential of 18 selected Lamiaceae representatives on yeast α-glucosidase and further translate experimental data into predicted human α-glucosidase inhibition. Since conventional antidiabetic drugs exhibit numerous unavoidable side effects, plant extracts are being increasingly explored as safer alternatives. Plant extracts were prepared by maceration, characterized by liquid chromatography-mass spectrometry (LC-MS), and subjected to in vitro determination of yeast α-glucosidase inhibitory potential, after which the kinetic study of one of the most potent extracts (Mentha × piperita) was performed. Docking simulations were done on both yeast and human α-glucosidase to explore binding modes and energies of the secondary metabolites identified in the examined extracts. Finally, the dynamical model has been constructed (adjR2 = 0.9885) and applied to explore extracts’ inhibition potential onto the human α-glucosidase. The constructed dynamical model revealed, for the first time, that different extracts of Satureja montana represent highly promising candidates for the prevention/treatment of diabetes mellitus. Therefore, the dynamical model described in this study represents a useful tool and a significant advance in the discovery of novel therapeutics with antidiabetic potential in humans.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling",
volume = "186",
doi = "10.1016/j.indcrop.2022.115200",
pages = "115200"
}

Oalđe Pavlović, M., Lunić, T., Graovac, S., Mandić, M., Repac, J., Gašić, U., Božić Nedeljković, B.,& Božić, B.. (2022). Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling. in Industrial Crops and Products
Elsevier B.V.., 186, 115200.
https://doi.org/10.1016/j.indcrop.2022.115200

Oalđe Pavlović M, Lunić T, Graovac S, Mandić M, Repac J, Gašić U, Božić Nedeljković B, Božić B. Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling. in Industrial Crops and Products. 2022;186:115200.
doi:10.1016/j.indcrop.2022.115200 .

Oalđe Pavlović, Mariana, Lunić, Tanja, Graovac, Stefan, Mandić, Marija, Repac, Jelena, Gašić, Uroš, Božić Nedeljković, Biljana, Božić, Bojan, "Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling" in Industrial Crops and Products, 186 (2022):115200,
https://doi.org/10.1016/j.indcrop.2022.115200 . .

TY  - JOUR
AU  - Mandić, Marija
AU  - Mitić, Katarina
AU  - Nedeljković, Predrag
AU  - Perić, Mina
AU  - Božić, Bojan
AU  - Lunić, Tanja
AU  - Bačić, Ana
AU  - Rajilić-Stojanović, Mirjana
AU  - Peković, Sanja
AU  - Božić Nedeljković, Biljana
PY  - 2022
UR  - https://www.mdpi.com/2072-6643/14/6/1273
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35334928
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8955508
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4940
AB  - The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.
PB  - Basel: MDPI
T2  - Nutrients
T1  - Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.
IS  - 6
VL  - 14
DO  - 10.3390/nu14061273
SP  - 1273
ER  - 

@article{
author = "Mandić, Marija and Mitić, Katarina and Nedeljković, Predrag and Perić, Mina and Božić, Bojan and Lunić, Tanja and Bačić, Ana and Rajilić-Stojanović, Mirjana and Peković, Sanja and Božić Nedeljković, Biljana",
year = "2022",
abstract = "The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.",
publisher = "Basel: MDPI",
journal = "Nutrients",
title = "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.",
number = "6",
volume = "14",
doi = "10.3390/nu14061273",
pages = "1273"
}

Mandić, M., Mitić, K., Nedeljković, P., Perić, M., Božić, B., Lunić, T., Bačić, A., Rajilić-Stojanović, M., Peković, S.,& Božić Nedeljković, B.. (2022). Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.. in Nutrients
Basel: MDPI., 14(6), 1273.
https://doi.org/10.3390/nu14061273

Mandić M, Mitić K, Nedeljković P, Perić M, Božić B, Lunić T, Bačić A, Rajilić-Stojanović M, Peković S, Božić Nedeljković B. Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.. in Nutrients. 2022;14(6):1273.
doi:10.3390/nu14061273 .

Mandić, Marija, Mitić, Katarina, Nedeljković, Predrag, Perić, Mina, Božić, Bojan, Lunić, Tanja, Bačić, Ana, Rajilić-Stojanović, Mirjana, Peković, Sanja, Božić Nedeljković, Biljana, "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis." in Nutrients, 14, no. 6 (2022):1273,
https://doi.org/10.3390/nu14061273 . .

TY  - JOUR
AU  - Oalđe Pavlović, Mariana
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Jovanović Marić, Jovana
AU  - Lunić, Tanja
AU  - Mandić, Marija
AU  - Kračun-Kolarević, Margareta
AU  - Živković, Jelena
AU  - Alimpić Aradski, Ana
AU  - Marin, Petar D.
AU  - Šavikin, Katarina
AU  - Vuković-Gačić, Branka
AU  - Božić Nedeljković, Biljana
AU  - Duletić-Laušević, Sonja
PY  - 2021
UR  - https://www.mdpi.com/2223-7747/10/11/2306
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4654
AB  - This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha × piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.
PB  - Basel: MDPI
T2  - Plants
T1  - A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species
IS  - 11
VL  - 10
DO  - 10.3390/plants10112306
SP  - 2306
ER  - 

@article{
author = "Oalđe Pavlović, Mariana and Kolarević, Stoimir and Đorđević, Jelena and Jovanović Marić, Jovana and Lunić, Tanja and Mandić, Marija and Kračun-Kolarević, Margareta and Živković, Jelena and Alimpić Aradski, Ana and Marin, Petar D. and Šavikin, Katarina and Vuković-Gačić, Branka and Božić Nedeljković, Biljana and Duletić-Laušević, Sonja",
year = "2021",
abstract = "This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha × piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species",
number = "11",
volume = "10",
doi = "10.3390/plants10112306",
pages = "2306"
}

Oalđe Pavlović, M., Kolarević, S., Đorđević, J., Jovanović Marić, J., Lunić, T., Mandić, M., Kračun-Kolarević, M., Živković, J., Alimpić Aradski, A., Marin, P. D., Šavikin, K., Vuković-Gačić, B., Božić Nedeljković, B.,& Duletić-Laušević, S.. (2021). A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants
Basel: MDPI., 10(11), 2306.
https://doi.org/10.3390/plants10112306

Oalđe Pavlović M, Kolarević S, Đorđević J, Jovanović Marić J, Lunić T, Mandić M, Kračun-Kolarević M, Živković J, Alimpić Aradski A, Marin PD, Šavikin K, Vuković-Gačić B, Božić Nedeljković B, Duletić-Laušević S. A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants. 2021;10(11):2306.
doi:10.3390/plants10112306 .

Oalđe Pavlović, Mariana, Kolarević, Stoimir, Đorđević, Jelena, Jovanović Marić, Jovana, Lunić, Tanja, Mandić, Marija, Kračun-Kolarević, Margareta, Živković, Jelena, Alimpić Aradski, Ana, Marin, Petar D., Šavikin, Katarina, Vuković-Gačić, Branka, Božić Nedeljković, Biljana, Duletić-Laušević, Sonja, "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species" in Plants, 10, no. 11 (2021):2306,
https://doi.org/10.3390/plants10112306 . .

TY  - CONF
AU  - Oalđe, Mariana
AU  - Kolarević, Stoimir
AU  - Mandić, Marija
AU  - Lunić, Tanja
AU  - Vuković-Gačić, Branka
AU  - Nedeljković, Biljana Božić
AU  - Duletić-Laušević, Sonja
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4858
AB  - Salvia officinalis (sage) and Salvia rosmarinus (previously Rosmarinus officinalis, rosemary) are commonly used medicinal plants from the Lamiaceae family, with strong antioxidant abilities. This study was carried out in several stages to comprehensively and gradually explore the antioxidant and antitumor mechanisms of their ethanolic extracts using different experimental approaches. Their in vitro antioxidant activity was assessed using ABTS assay. Plasmid pUC19 E. coli XL1-Blue was used to evaluate their genoprotective activity, while SOS/umuC assay on Salmonella typhimurium TA1535/pSK1002 and Comet assay on human lung fibroblast cell line (MRC-5) were employed to examine their antigenotoxic potential. Furthermore, their antitumor mechanisms were analyzed on the colorectal cancer cell line (HCT-116) by testing their impact on the cells’ viability (MTT assay), as well as the production of reactive oxygen species (ROS) (NBT test) and nitric oxide (NO) (Griess assay). The results showed that sage extract had slightly higher ABTS-scavenging and antigenotoxic activities in the bacterial model when compared to the rosemary extract, however, opposite results were obtained for the acellular model. Moreover, both extracts protected the MRC-5 cells from the induced genomic damages. As for tumor cells, sage extract reduced their viability by approximately 10 %. Additionally, both extracts significantly reduced the ROS production, while also increasing the NO production, especially the rosemary extract. These results delivered novel information about the nature of antioxidant effects of sage and rosemary ethanolic extracts. They provided substantial protection of the acellular, bacterial and healthy human cellular models by employing radical-scavenging and antigenotoxic mechanisms. At the same time, both extracts exhibited antitumor activity towards colorectal cancer cells in vitro that could be mediated by inducing an increase in NO production. With that being said, sage and rosemary ethanolic extracts represent potent genoprotective, antioxidant and antitumor agents. However, their exact mechanisms of action remain to be further explored and clarified
PB  - Elsevier BV
PB  - Elsevier Inc.
C3  - Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia
T1  - Different levels of exploring the antioxidant and antitumor potential of Salvia officinalis and Salvia rosmarinus
VL  - 177
DO  - 10.1016/j.freeradbiomed.2021.08.131
SP  - S95
ER  - 

@conference{
author = "Oalđe, Mariana and Kolarević, Stoimir and Mandić, Marija and Lunić, Tanja and Vuković-Gačić, Branka and Nedeljković, Biljana Božić and Duletić-Laušević, Sonja",
year = "2021",
abstract = "Salvia officinalis (sage) and Salvia rosmarinus (previously Rosmarinus officinalis, rosemary) are commonly used medicinal plants from the Lamiaceae family, with strong antioxidant abilities. This study was carried out in several stages to comprehensively and gradually explore the antioxidant and antitumor mechanisms of their ethanolic extracts using different experimental approaches. Their in vitro antioxidant activity was assessed using ABTS assay. Plasmid pUC19 E. coli XL1-Blue was used to evaluate their genoprotective activity, while SOS/umuC assay on Salmonella typhimurium TA1535/pSK1002 and Comet assay on human lung fibroblast cell line (MRC-5) were employed to examine their antigenotoxic potential. Furthermore, their antitumor mechanisms were analyzed on the colorectal cancer cell line (HCT-116) by testing their impact on the cells’ viability (MTT assay), as well as the production of reactive oxygen species (ROS) (NBT test) and nitric oxide (NO) (Griess assay). The results showed that sage extract had slightly higher ABTS-scavenging and antigenotoxic activities in the bacterial model when compared to the rosemary extract, however, opposite results were obtained for the acellular model. Moreover, both extracts protected the MRC-5 cells from the induced genomic damages. As for tumor cells, sage extract reduced their viability by approximately 10 %. Additionally, both extracts significantly reduced the ROS production, while also increasing the NO production, especially the rosemary extract. These results delivered novel information about the nature of antioxidant effects of sage and rosemary ethanolic extracts. They provided substantial protection of the acellular, bacterial and healthy human cellular models by employing radical-scavenging and antigenotoxic mechanisms. At the same time, both extracts exhibited antitumor activity towards colorectal cancer cells in vitro that could be mediated by inducing an increase in NO production. With that being said, sage and rosemary ethanolic extracts represent potent genoprotective, antioxidant and antitumor agents. However, their exact mechanisms of action remain to be further explored and clarified",
publisher = "Elsevier BV, Elsevier Inc.",
journal = "Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia",
title = "Different levels of exploring the antioxidant and antitumor potential of Salvia officinalis and Salvia rosmarinus",
volume = "177",
doi = "10.1016/j.freeradbiomed.2021.08.131",
pages = "S95"
}

Oalđe, M., Kolarević, S., Mandić, M., Lunić, T., Vuković-Gačić, B., Nedeljković, B. B.,& Duletić-Laušević, S.. (2021). Different levels of exploring the antioxidant and antitumor potential of Salvia officinalis and Salvia rosmarinus. in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia
Elsevier BV., 177, S95.
https://doi.org/10.1016/j.freeradbiomed.2021.08.131

Oalđe M, Kolarević S, Mandić M, Lunić T, Vuković-Gačić B, Nedeljković BB, Duletić-Laušević S. Different levels of exploring the antioxidant and antitumor potential of Salvia officinalis and Salvia rosmarinus. in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia. 2021;177:S95.
doi:10.1016/j.freeradbiomed.2021.08.131 .

Oalđe, Mariana, Kolarević, Stoimir, Mandić, Marija, Lunić, Tanja, Vuković-Gačić, Branka, Nedeljković, Biljana Božić, Duletić-Laušević, Sonja, "Different levels of exploring the antioxidant and antitumor potential of Salvia officinalis and Salvia rosmarinus" in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia, 177 (2021):S95,
https://doi.org/10.1016/j.freeradbiomed.2021.08.131 . .

TY  - JOUR
AU  - Lunić, Tanja M.
AU  - Oalđe, Mariana M.
AU  - Mandić, Marija R.
AU  - Sabovljević, Aneta D
AU  - Sabovljević, Marko S.
AU  - Gašić, Uroš
AU  - Duletić-Laušević, Sonja N.
AU  - Božić, Bojan Dj.
AU  - Božić Nedeljković, Biljana Dj.
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/15/3343
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32717985
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7436104
UR  - https://radar.ibiss.bg.ac.rs/123456789/3851
AB  - Recently, there has been an increasing interest in the chemistry and biological potential of mosses, since a large number of biologically active compounds have been found within these species. This study aimed at examining the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory/antineurodegenerative, and antitumor activities) of moss Hypnum cupressiforme Hedw. extracts. Corresponding extracts have been obtained applying Soxhlet extractor. The chemical characterization was performed using spectrophotometric assays and liquid chromatography-mass spectrometry (LC-MS). The extracts were analyzed for antioxidant activity and for inhibitory activities on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase. Additionally, extracts were tested against four cell lines-MRC-5, BV2, HCT-116, and MDA-MB-231-for antitumor and anti-inflammatory activities. Chemical analysis of extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. Major compounds identified by LC-MS in H. cupressiforme were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. According to biochemical assays the investigated extracts exhibited significant immunomodulatory potential. Significant antiproliferative potential against MDA-MB-231 cells has been observed together with the promising anti-neuroinflammatory application. The obtained data suggest that moss H. cupressiforme is a valuable natural source of biologically active compounds with potential application in the pharmaceutical industry.
PB  - MDPI AG
T2  - Molecules (Basel, Switzerland)
T1  - Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw.
IS  - 15
VL  - 25
DO  - 10.3390/molecules25153343
SP  - 3343
ER  - 

@article{
author = "Lunić, Tanja M. and Oalđe, Mariana M. and Mandić, Marija R. and Sabovljević, Aneta D and Sabovljević, Marko S. and Gašić, Uroš and Duletić-Laušević, Sonja N. and Božić, Bojan Dj. and Božić Nedeljković, Biljana Dj.",
year = "2020",
abstract = "Recently, there has been an increasing interest in the chemistry and biological potential of mosses, since a large number of biologically active compounds have been found within these species. This study aimed at examining the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory/antineurodegenerative, and antitumor activities) of moss Hypnum cupressiforme Hedw. extracts. Corresponding extracts have been obtained applying Soxhlet extractor. The chemical characterization was performed using spectrophotometric assays and liquid chromatography-mass spectrometry (LC-MS). The extracts were analyzed for antioxidant activity and for inhibitory activities on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase. Additionally, extracts were tested against four cell lines-MRC-5, BV2, HCT-116, and MDA-MB-231-for antitumor and anti-inflammatory activities. Chemical analysis of extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. Major compounds identified by LC-MS in H. cupressiforme were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. According to biochemical assays the investigated extracts exhibited significant immunomodulatory potential. Significant antiproliferative potential against MDA-MB-231 cells has been observed together with the promising anti-neuroinflammatory application. The obtained data suggest that moss H. cupressiforme is a valuable natural source of biologically active compounds with potential application in the pharmaceutical industry.",
publisher = "MDPI AG",
journal = "Molecules (Basel, Switzerland)",
title = "Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw.",
number = "15",
volume = "25",
doi = "10.3390/molecules25153343",
pages = "3343"
}

Lunić, T. M., Oalđe, M. M., Mandić, M. R., Sabovljević, A. D., Sabovljević, M. S., Gašić, U., Duletić-Laušević, S. N., Božić, B. Dj.,& Božić Nedeljković, B. Dj.. (2020). Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw.. in Molecules (Basel, Switzerland)
MDPI AG., 25(15), 3343.
https://doi.org/10.3390/molecules25153343

Lunić TM, Oalđe MM, Mandić MR, Sabovljević AD, Sabovljević MS, Gašić U, Duletić-Laušević SN, Božić BD, Božić Nedeljković BD. Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw.. in Molecules (Basel, Switzerland). 2020;25(15):3343.
doi:10.3390/molecules25153343 .

Lunić, Tanja M., Oalđe, Mariana M., Mandić, Marija R., Sabovljević, Aneta D, Sabovljević, Marko S., Gašić, Uroš, Duletić-Laušević, Sonja N., Božić, Bojan Dj., Božić Nedeljković, Biljana Dj., "Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw." in Molecules (Basel, Switzerland), 25, no. 15 (2020):3343,
https://doi.org/10.3390/molecules25153343 . .

TY  - CONF
AU  - Lunić, Tanja
AU  - Oalđe, Mariana
AU  - Mandić, Marija
AU  - Sabovljević, Aneta
AU  - Sabovljević, Marko
AU  - Gašić, Uroš
AU  - Duletić-Laušević, Sonja
AU  - Božić, Bojan
AU  - Božić Nedeljković, Biljana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4106
AB  - Mosses have recently been recognized as promising sources of novel biologically active compounds applicable in the prevention of numerous pathological conditions or as potent adjuvant therapeutics. This study aimed to examine the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory, antineurodegenerative, and antitumor activities) of the moss H. cupressiforme extracts. The corresponding extracts were obtained using solvents of different polarities and their mixtures with Soxhlet extractor and further characterized by spectrophotometric assays and liquid chromatography coupled to mass spectrometry (LC-MS). The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), total reduction power, and β-carotene bleaching assays. Further on, the inhibitory activity on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase was tested. Additionally, biocompatibility, antitumor, and anti-inflammatory potential were tested on MRC-5, HCT-116, MDA-MB-231, and BV2 cells, respectively.

Chemical analysis of the moss extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. The major compounds identified by LC-MS in H. cupressiforme extracts were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. Biochemical assays revealed the significant immunomodulatory potential of examined extracts. Moreover, significant antiproliferative potential against human breast cancer cells – MDA-MB-231, with the proliferation inhibition rate up to 50%, and acceptable biocompatibility was observed. Also, a significant decrease in NO production, observed in lipopolysaccharide-stimulated BV2 cells, implies potential anti-neuroinflammatory application. Overall, obtained results qualify the moss H. cupressiforme as a highly promising candidate for more detailed examination and also putative therapeutical application.
PB  - Basel: MDPI
C3  - 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.
T1  - Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts
DO  - 10.3390/ECMC2020-07282
ER  - 

@conference{
author = "Lunić, Tanja and Oalđe, Mariana and Mandić, Marija and Sabovljević, Aneta and Sabovljević, Marko and Gašić, Uroš and Duletić-Laušević, Sonja and Božić, Bojan and Božić Nedeljković, Biljana",
year = "2020",
abstract = "Mosses have recently been recognized as promising sources of novel biologically active compounds applicable in the prevention of numerous pathological conditions or as potent adjuvant therapeutics. This study aimed to examine the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory, antineurodegenerative, and antitumor activities) of the moss H. cupressiforme extracts. The corresponding extracts were obtained using solvents of different polarities and their mixtures with Soxhlet extractor and further characterized by spectrophotometric assays and liquid chromatography coupled to mass spectrometry (LC-MS). The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), total reduction power, and β-carotene bleaching assays. Further on, the inhibitory activity on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase was tested. Additionally, biocompatibility, antitumor, and anti-inflammatory potential were tested on MRC-5, HCT-116, MDA-MB-231, and BV2 cells, respectively.

Chemical analysis of the moss extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. The major compounds identified by LC-MS in H. cupressiforme extracts were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. Biochemical assays revealed the significant immunomodulatory potential of examined extracts. Moreover, significant antiproliferative potential against human breast cancer cells – MDA-MB-231, with the proliferation inhibition rate up to 50%, and acceptable biocompatibility was observed. Also, a significant decrease in NO production, observed in lipopolysaccharide-stimulated BV2 cells, implies potential anti-neuroinflammatory application. Overall, obtained results qualify the moss H. cupressiforme as a highly promising candidate for more detailed examination and also putative therapeutical application.",
publisher = "Basel: MDPI",
journal = "6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.",
title = "Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts",
doi = "10.3390/ECMC2020-07282"
}

Lunić, T., Oalđe, M., Mandić, M., Sabovljević, A., Sabovljević, M., Gašić, U., Duletić-Laušević, S., Božić, B.,& Božić Nedeljković, B.. (2020). Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts. in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.
Basel: MDPI..
https://doi.org/10.3390/ECMC2020-07282

Lunić T, Oalđe M, Mandić M, Sabovljević A, Sabovljević M, Gašić U, Duletić-Laušević S, Božić B, Božić Nedeljković B. Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts. in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.. 2020;.
doi:10.3390/ECMC2020-07282 .

Lunić, Tanja, Oalđe, Mariana, Mandić, Marija, Sabovljević, Aneta, Sabovljević, Marko, Gašić, Uroš, Duletić-Laušević, Sonja, Božić, Bojan, Božić Nedeljković, Biljana, "Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts" in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online. (2020),
https://doi.org/10.3390/ECMC2020-07282 . .

TY  - CONF
AU  - Oalđe, Mariana
AU  - Mandić, Marija
AU  - Gašić, Uroš
AU  - Lunić, Tanja
AU  - Kolarević, Stoimir
AU  - Vuković-Gačić, Branka
AU  - Alimpić Aradski, Ana
AU  - Marin, Petar
AU  - Božić Nedeljković, Biljana
AU  - Duletić-Laušević, Sonja
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4104
AB  - The physiological imbalance between the production and accumulation of reactive oxygen species (ROS), known as oxidative stress, is responsible for developing and progression of a plethora of diseases – cancer, among others. Since plants are recognized as potential antioxidant agents, the aim of this study was to chemically characterize ethanolic extracts of 18 species from Lamiaceae family using liquid chromatography-mass spectrometry (LC-MS), followed by testing the antioxidant activity using ABTS assay. Biocompatibility of the investigated extracts was assessed by MTT assay on normal human lung fibroblasts (MRC-5). Finally, antiproliferative activity was evaluated using MTT assay on human colorectal cancer (HCT-116) cell line, while the effects of extracts on superoxide anion radical (O2•-) production were tested using NBT assay. Melissa officinalis, Mentha piperita, Origanum majorana, O. vulgare, and Thymus vulgaris showed the highest antioxidant activity against ABTS radicals. LC-MS analysis indicated that these extracts mostly contain caffeic, protocatechuic and p-coumaric acids. The extracts were biocompatible with MRC-5 cell line. Interestingly, Lavandula angustifolia and Ocimum basilicum extracts, which were less active against ABTS radicals, showed a significant antiproliferative effect against HCT-116. Moreover, L. angustifolia and T. vulgaris extracts significantly stimulated the production of O2•- in HCT-116, indicating potential antitumor activity. The extracts that expressed antiproliferative effect and stimulated ROS production contain higher amounts of rosmarinic, caffeic and chlorogenic acids, naringin, luteolin, apigenin and cirsimaritin, indicating that some of them, or more probably their synergistic effects, are responsible for the extracts’ ability to moderate the production of ROS in HCT-116 cells.
PB  - Basel: MDPI
C3  - 6th International Electronic Conference on Medicinal Chemistry: Online; 2020 Nov 1-30
T1  - Chemical characterization of Lamiaceae plant extracts – the quest for novel immunomodulators
DO  - 10.3390/ECMC2020-07280
ER  - 

@conference{
author = "Oalđe, Mariana and Mandić, Marija and Gašić, Uroš and Lunić, Tanja and Kolarević, Stoimir and Vuković-Gačić, Branka and Alimpić Aradski, Ana and Marin, Petar and Božić Nedeljković, Biljana and Duletić-Laušević, Sonja",
year = "2020",
abstract = "The physiological imbalance between the production and accumulation of reactive oxygen species (ROS), known as oxidative stress, is responsible for developing and progression of a plethora of diseases – cancer, among others. Since plants are recognized as potential antioxidant agents, the aim of this study was to chemically characterize ethanolic extracts of 18 species from Lamiaceae family using liquid chromatography-mass spectrometry (LC-MS), followed by testing the antioxidant activity using ABTS assay. Biocompatibility of the investigated extracts was assessed by MTT assay on normal human lung fibroblasts (MRC-5). Finally, antiproliferative activity was evaluated using MTT assay on human colorectal cancer (HCT-116) cell line, while the effects of extracts on superoxide anion radical (O2•-) production were tested using NBT assay. Melissa officinalis, Mentha piperita, Origanum majorana, O. vulgare, and Thymus vulgaris showed the highest antioxidant activity against ABTS radicals. LC-MS analysis indicated that these extracts mostly contain caffeic, protocatechuic and p-coumaric acids. The extracts were biocompatible with MRC-5 cell line. Interestingly, Lavandula angustifolia and Ocimum basilicum extracts, which were less active against ABTS radicals, showed a significant antiproliferative effect against HCT-116. Moreover, L. angustifolia and T. vulgaris extracts significantly stimulated the production of O2•- in HCT-116, indicating potential antitumor activity. The extracts that expressed antiproliferative effect and stimulated ROS production contain higher amounts of rosmarinic, caffeic and chlorogenic acids, naringin, luteolin, apigenin and cirsimaritin, indicating that some of them, or more probably their synergistic effects, are responsible for the extracts’ ability to moderate the production of ROS in HCT-116 cells.",
publisher = "Basel: MDPI",
journal = "6th International Electronic Conference on Medicinal Chemistry: Online; 2020 Nov 1-30",
title = "Chemical characterization of Lamiaceae plant extracts – the quest for novel immunomodulators",
doi = "10.3390/ECMC2020-07280"
}

Oalđe, M., Mandić, M., Gašić, U., Lunić, T., Kolarević, S., Vuković-Gačić, B., Alimpić Aradski, A., Marin, P., Božić Nedeljković, B.,& Duletić-Laušević, S.. (2020). Chemical characterization of Lamiaceae plant extracts – the quest for novel immunomodulators. in 6th International Electronic Conference on Medicinal Chemistry: Online; 2020 Nov 1-30
Basel: MDPI..
https://doi.org/10.3390/ECMC2020-07280

Oalđe M, Mandić M, Gašić U, Lunić T, Kolarević S, Vuković-Gačić B, Alimpić Aradski A, Marin P, Božić Nedeljković B, Duletić-Laušević S. Chemical characterization of Lamiaceae plant extracts – the quest for novel immunomodulators. in 6th International Electronic Conference on Medicinal Chemistry: Online; 2020 Nov 1-30. 2020;.
doi:10.3390/ECMC2020-07280 .

Oalđe, Mariana, Mandić, Marija, Gašić, Uroš, Lunić, Tanja, Kolarević, Stoimir, Vuković-Gačić, Branka, Alimpić Aradski, Ana, Marin, Petar, Božić Nedeljković, Biljana, Duletić-Laušević, Sonja, "Chemical characterization of Lamiaceae plant extracts – the quest for novel immunomodulators" in 6th International Electronic Conference on Medicinal Chemistry: Online; 2020 Nov 1-30 (2020),
https://doi.org/10.3390/ECMC2020-07280 . .