TY  - CONF
AU  - Mileusnić, Dina
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Kulaš, Jelena
AU  - Vukojević, Vesna
AU  - Đurđić, Slađana
AU  - Mutić, Jelena
AU  - Kataranovski, Milena
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4855
AB  - Epidemiological studies indicate a possible link between cadmium (Cd) ingested through food and various pathologies / diseases. There is no such data when it comes to skin, although the deposition of this metal in hair is used as a biomarker of human exposure. Only one study has shown the effect of orally administered Cd on wound healing, but the effects on other homeostatic skin activities, including immune, have not been investigated. In order to investigate the potential immunomodulatory effect of this heavy metal on the skin, DA strain rats were exposed to subchronic (30 days) oral intake of cadmium (in drinking water, 5 mg / l and 50 mg / l).Cd was deposited in this tissue and led to oxidative stress, judging by the increase in antioxidant enzymes (SOD and CAT), structural changes in the skin, as well as the presence / activation of innate immune cells. Higher concentrations of deposited Cd in the epidermis compared to the dermis resulted in a stress response at the epidermal cell (EC) level, manifested by increased mRNA expression for metallothioneins (MT-1 and MT-2) and decreased GSH after a higher dose of CdThis metal also led to the manifestation of signs of inflammation, judging by the increased expression of mRNA for iNOS and IL-1β and the production of NO and IL-1β, at a lower dose, and mRNA and IL-6 and TNF protein product at a higher dose. On the other hand, Cd led to increased production of the regulatory cytokine IL-10 (at higher doses). Increased IL-17 production was observed in EC cultures isolated from the skin of rats exposed to 50 mg / l, while the amount of cytokine produced further increased in EC co-culture with lymph node lymphocytes after ConA stimulation (both doses).Cd present in the skin also increased the ability of the EC to respond to the application of the contact allergen DNCB, judging by the increase in IL-6 and TNF (at both doses), while IL-1β remained unchanged. The presented results show a generally proinflammatory effect of Cd on epidermal cells and indicate the potential of orally ingested Cd to modulate immune responses in the skin.
PB  - Belgrade: Immunological Society of Serbia
C3  - Svetski dan imunologije; 2018 Apr 26; Belgrade, Serbia
T1  - Oral cadmium intake affect skin immune ractivity
T1  - Oralni unos kadmijuma utiče na imunsku reaktivnost kože
SP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4855
ER  - 

@conference{
author = "Mileusnić, Dina and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Ninkov, Marina and Kulaš, Jelena and Vukojević, Vesna and Đurđić, Slađana and Mutić, Jelena and Kataranovski, Milena",
year = "2018",
abstract = "Epidemiological studies indicate a possible link between cadmium (Cd) ingested through food and various pathologies / diseases. There is no such data when it comes to skin, although the deposition of this metal in hair is used as a biomarker of human exposure. Only one study has shown the effect of orally administered Cd on wound healing, but the effects on other homeostatic skin activities, including immune, have not been investigated. In order to investigate the potential immunomodulatory effect of this heavy metal on the skin, DA strain rats were exposed to subchronic (30 days) oral intake of cadmium (in drinking water, 5 mg / l and 50 mg / l).Cd was deposited in this tissue and led to oxidative stress, judging by the increase in antioxidant enzymes (SOD and CAT), structural changes in the skin, as well as the presence / activation of innate immune cells. Higher concentrations of deposited Cd in the epidermis compared to the dermis resulted in a stress response at the epidermal cell (EC) level, manifested by increased mRNA expression for metallothioneins (MT-1 and MT-2) and decreased GSH after a higher dose of CdThis metal also led to the manifestation of signs of inflammation, judging by the increased expression of mRNA for iNOS and IL-1β and the production of NO and IL-1β, at a lower dose, and mRNA and IL-6 and TNF protein product at a higher dose. On the other hand, Cd led to increased production of the regulatory cytokine IL-10 (at higher doses). Increased IL-17 production was observed in EC cultures isolated from the skin of rats exposed to 50 mg / l, while the amount of cytokine produced further increased in EC co-culture with lymph node lymphocytes after ConA stimulation (both doses).Cd present in the skin also increased the ability of the EC to respond to the application of the contact allergen DNCB, judging by the increase in IL-6 and TNF (at both doses), while IL-1β remained unchanged. The presented results show a generally proinflammatory effect of Cd on epidermal cells and indicate the potential of orally ingested Cd to modulate immune responses in the skin.",
publisher = "Belgrade: Immunological Society of Serbia",
journal = "Svetski dan imunologije; 2018 Apr 26; Belgrade, Serbia",
title = "Oral cadmium intake affect skin immune ractivity, Oralni unos kadmijuma utiče na imunsku reaktivnost kože",
pages = "24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4855"
}

Mileusnić, D., Popov Aleksandrov, A., Mirkov, I., Ninkov, M., Kulaš, J., Vukojević, V., Đurđić, S., Mutić, J.,& Kataranovski, M.. (2018). Oral cadmium intake affect skin immune ractivity. in Svetski dan imunologije; 2018 Apr 26; Belgrade, Serbia
Belgrade: Immunological Society of Serbia., 24.
https://hdl.handle.net/21.15107/rcub_ibiss_4855

Mileusnić D, Popov Aleksandrov A, Mirkov I, Ninkov M, Kulaš J, Vukojević V, Đurđić S, Mutić J, Kataranovski M. Oral cadmium intake affect skin immune ractivity. in Svetski dan imunologije; 2018 Apr 26; Belgrade, Serbia. 2018;:24.
https://hdl.handle.net/21.15107/rcub_ibiss_4855 .

Mileusnić, Dina, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Ninkov, Marina, Kulaš, Jelena, Vukojević, Vesna, Đurđić, Slađana, Mutić, Jelena, Kataranovski, Milena, "Oral cadmium intake affect skin immune ractivity" in Svetski dan imunologije; 2018 Apr 26; Belgrade, Serbia (2018):24,
https://hdl.handle.net/21.15107/rcub_ibiss_4855 .

TY  - JOUR
AU  - Prokić, Marko
AU  - Borković-Mitić, Slavica
AU  - Krizmanić, Imre
AU  - Mutić, Jelena
AU  - Trifković, Jelena
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5930
AB  - Metals are involved in the formation of reactive
oxygen species and can induce oxidative stress. The aim of
this study was to assess the effects of several metals on
oxidative stress in the skin and muscle of the Pelophylax
esculentus “complex” frogs (parental species Pelophylax
ridibundus, Pelophylax lessonae, and their hybrid Pelophylax esculentus) that inhabit the wetland Obedska Bara in
Serbia, and the potential use of these species as bioindicator
organisms in biomonitoring studies. The biomarkers of
oxidative stress (SOD, CAT, GSH-Px, GR, GST activities
and GSH, SH concentrations) and cholinesterase activity
were investigated. The concentrations of nine metals (Fe,
Cu, Zn, As, Cd, Cr, Hg, Ni, and Pb) were measured in the
water and tissues. Correlations were established between
metals and biomarkers in the tissues. The results of metal
accumulation distinguished the skin of P. lessonae and
muscle of P. ridibundus from other P. esculentus complex
species. The oxidative stress biomarkers observed in P.
ridibundus and P. esculentus had greater similarity than in
P. lessonae. The P. lessonae displayed the highest number
of correlations between biomarkers and metals. The results
of tissue responses revealed that skin was more susceptible
to metal-induced oxidative stress, with only exception of
As. In the light of these findings, we can suggest the use of
P. esculentus complex species as a biomonitoring species in
studies of metal accumulation and metal-induced oxidative
stress, but with special emphasis on P. lessonae.
PB  - Dordrecht: Springer
T2  - Ecotoxicology
T1  - Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex
IS  - 8
VL  - 25
DO  - 10.1007/s10646-016-1707-x
SP  - 1531
EP  - 1542
ER  - 

@article{
author = "Prokić, Marko and Borković-Mitić, Slavica and Krizmanić, Imre and Mutić, Jelena and Trifković, Jelena and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Pavlović, Slađan and Saičić, Zorica",
year = "2016",
abstract = "Metals are involved in the formation of reactive
oxygen species and can induce oxidative stress. The aim of
this study was to assess the effects of several metals on
oxidative stress in the skin and muscle of the Pelophylax
esculentus “complex” frogs (parental species Pelophylax
ridibundus, Pelophylax lessonae, and their hybrid Pelophylax esculentus) that inhabit the wetland Obedska Bara in
Serbia, and the potential use of these species as bioindicator
organisms in biomonitoring studies. The biomarkers of
oxidative stress (SOD, CAT, GSH-Px, GR, GST activities
and GSH, SH concentrations) and cholinesterase activity
were investigated. The concentrations of nine metals (Fe,
Cu, Zn, As, Cd, Cr, Hg, Ni, and Pb) were measured in the
water and tissues. Correlations were established between
metals and biomarkers in the tissues. The results of metal
accumulation distinguished the skin of P. lessonae and
muscle of P. ridibundus from other P. esculentus complex
species. The oxidative stress biomarkers observed in P.
ridibundus and P. esculentus had greater similarity than in
P. lessonae. The P. lessonae displayed the highest number
of correlations between biomarkers and metals. The results
of tissue responses revealed that skin was more susceptible
to metal-induced oxidative stress, with only exception of
As. In the light of these findings, we can suggest the use of
P. esculentus complex species as a biomonitoring species in
studies of metal accumulation and metal-induced oxidative
stress, but with special emphasis on P. lessonae.",
publisher = "Dordrecht: Springer",
journal = "Ecotoxicology",
title = "Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex",
number = "8",
volume = "25",
doi = "10.1007/s10646-016-1707-x",
pages = "1531-1542"
}

Prokić, M., Borković-Mitić, S., Krizmanić, I., Mutić, J., Trifković, J., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Pavlović, S.,& Saičić, Z.. (2016). Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex. in Ecotoxicology
Dordrecht: Springer., 25(8), 1531-1542.
https://doi.org/10.1007/s10646-016-1707-x

Prokić M, Borković-Mitić S, Krizmanić I, Mutić J, Trifković J, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Pavlović S, Saičić Z. Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex. in Ecotoxicology. 2016;25(8):1531-1542.
doi:10.1007/s10646-016-1707-x .

Prokić, Marko, Borković-Mitić, Slavica, Krizmanić, Imre, Mutić, Jelena, Trifković, Jelena, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Pavlović, Slađan, Saičić, Zorica, "Bioaccumulation and effects of metals on oxidative stress and neurotoxicity parameters in the frogs from the Pelophylax esculentus complex" in Ecotoxicology, 25, no. 8 (2016):1531-1542,
https://doi.org/10.1007/s10646-016-1707-x . .