TY  - JOUR
AU  - Venturelli, Leonardo
AU  - Kohler, Anne-Céline
AU  - Stupar, Petar
AU  - Villalba, Maria I.
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
AU  - Bertacchi, Massimiliano
AU  - Dinarelli, Simone
AU  - Girasole, Marco
AU  - Pešić, Milica
AU  - Banković, Jasna
AU  - Vela, Maria E.
AU  - Yantorno, Osvaldo
AU  - Willaert, Ronnie
AU  - Dietler, Giovanni
AU  - Longo, Giovanni
AU  - Kasas, Sandor
PY  - 2020
UR  - http://doi.wiley.com/10.1002/jmr.2849
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3641
AB  - The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.
T2  - Journal of Molecular Recognition
T1  - A perspective view on the nanomotion detection of living organisms and its features
DO  - 10.1002/jmr.2849
SP  - e2849
ER  - 

@article{
author = "Venturelli, Leonardo and Kohler, Anne-Céline and Stupar, Petar and Villalba, Maria I. and Kalauzi, Aleksandar and Radotić, Ksenija and Bertacchi, Massimiliano and Dinarelli, Simone and Girasole, Marco and Pešić, Milica and Banković, Jasna and Vela, Maria E. and Yantorno, Osvaldo and Willaert, Ronnie and Dietler, Giovanni and Longo, Giovanni and Kasas, Sandor",
year = "2020",
abstract = "The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.",
journal = "Journal of Molecular Recognition",
title = "A perspective view on the nanomotion detection of living organisms and its features",
doi = "10.1002/jmr.2849",
pages = "e2849"
}

Venturelli, L., Kohler, A., Stupar, P., Villalba, M. I., Kalauzi, A., Radotić, K., Bertacchi, M., Dinarelli, S., Girasole, M., Pešić, M., Banković, J., Vela, M. E., Yantorno, O., Willaert, R., Dietler, G., Longo, G.,& Kasas, S.. (2020). A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition, e2849.
https://doi.org/10.1002/jmr.2849

Venturelli L, Kohler A, Stupar P, Villalba MI, Kalauzi A, Radotić K, Bertacchi M, Dinarelli S, Girasole M, Pešić M, Banković J, Vela ME, Yantorno O, Willaert R, Dietler G, Longo G, Kasas S. A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition. 2020;:e2849.
doi:10.1002/jmr.2849 .

Venturelli, Leonardo, Kohler, Anne-Céline, Stupar, Petar, Villalba, Maria I., Kalauzi, Aleksandar, Radotić, Ksenija, Bertacchi, Massimiliano, Dinarelli, Simone, Girasole, Marco, Pešić, Milica, Banković, Jasna, Vela, Maria E., Yantorno, Osvaldo, Willaert, Ronnie, Dietler, Giovanni, Longo, Giovanni, Kasas, Sandor, "A perspective view on the nanomotion detection of living organisms and its features" in Journal of Molecular Recognition (2020):e2849,
https://doi.org/10.1002/jmr.2849 . .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Milosevic, Vuk
AU  - Živković, Miroslava
AU  - Stojanov, Dragan
AU  - Milojković, Olga
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2017
UR  - https://peerj.com/articles/3839
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2874
AB  - Background. We investigated EEG rhythms, particularly alpha activity, and their relationship to post-stroke neuropathology and cognitive functions in the subacute and chronic stages of minor strokes. Methods. We included 10 patients with right middle cerebral artery (MCA) ischemic strokes a nd 11 healthy controls. All the assessments of stroke patients were done both in the subacute and chronic stages. Neurological impairment was measured using the National Institute of Health Stroke Scale (NIHSS), whereas cognitive functions were assessed using the Montreal Cognitive Assessment (MoCA) and MoCA memory index (MoCA-MIS). The EEG was recorded using a 19 channel EEG system with standard EEG electrode placement. In particular, we analyzed the EEGs derived from the four lateral frontal (F3, F7, F4, F8), and corresponding lateral posterior (P3, P4, T5, T6) electrodes. Quantitative EEG analysis included: the group FFT spectra, the weighted average of alpha frequency (α AVG), the group probability density distributions of all conventional EEG frequency band relative amplitudes (EEG microstructure), the inter- and intra-hemispheric coherences, and the topographic distribution of alpha carrier frequency phase potentials (PPs). Statistical analysis was done using a Kruskal-Wallis ANOVA with a post-hoc Mann-WhitneyU two-tailed test, and Spearman's correlation. Results. We demonstrated transient cognitive impairment alongside a slower alpha fre- quency (αAVG) in the subacute right MCA stroke patients vs. the controls. This slower alpha frequency showed no amplitude change, but was highly synchronized intra- hemispherically, overlying the ipsi-lesional hemisphere, and inter-hemispherically, overlying the frontal cortex. In addition, the disturbances in EEG alpha activity in subacute stroke patients were expressed as a decrease in alpha PPs over the frontal cortex and an altered "alpha flow", indicating the sustained augmentation of inter- hemispheric interactions. Although the stroke induced slower alpha was a transient phenomenon, the increased alpha intra-hemispheric synchronization, overlying the ipsi-lesional hemisphere, the increased alpha F3-F4 inter-hemispheric synchronization, the delayed alpha waves, and the newly established inter-hemispheric "alpha flow" within the frontal cortex, remained as a permanent consequence of the minor stroke. This newly established frontal inter-hemispheric "alpha flow" represented a permanent consequence of the ``hidden" stroke neuropathology, despite the fact that cognitive impairment has been returned to the control values. All the detected permanent changes at the EEG level with no cognitive impairment after a minor stroke could be a way for the brain to compensate for the lesion and restore the lost function. Discussion. Our study indicates slower EEG alpha generation, synchronization and ``flow" as potential biomarkers of cognitive impairment onset and/or compensatory post-stroke re-organizational processes.
T2  - PeerJ
T1  - Slower EEG alpha generation, synchronization and “flow”—possible biomarkers of cognitive impairment and neuropathology of minor stroke
IS  - 9
VL  - 5
DO  - 10.7717/peerj.3839
SP  - e3839
EP  - e3839
ER  - 

@article{
author = "Petrović, Jelena and Milosevic, Vuk and Živković, Miroslava and Stojanov, Dragan and Milojković, Olga and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2017",
abstract = "Background. We investigated EEG rhythms, particularly alpha activity, and their relationship to post-stroke neuropathology and cognitive functions in the subacute and chronic stages of minor strokes. Methods. We included 10 patients with right middle cerebral artery (MCA) ischemic strokes a nd 11 healthy controls. All the assessments of stroke patients were done both in the subacute and chronic stages. Neurological impairment was measured using the National Institute of Health Stroke Scale (NIHSS), whereas cognitive functions were assessed using the Montreal Cognitive Assessment (MoCA) and MoCA memory index (MoCA-MIS). The EEG was recorded using a 19 channel EEG system with standard EEG electrode placement. In particular, we analyzed the EEGs derived from the four lateral frontal (F3, F7, F4, F8), and corresponding lateral posterior (P3, P4, T5, T6) electrodes. Quantitative EEG analysis included: the group FFT spectra, the weighted average of alpha frequency (α AVG), the group probability density distributions of all conventional EEG frequency band relative amplitudes (EEG microstructure), the inter- and intra-hemispheric coherences, and the topographic distribution of alpha carrier frequency phase potentials (PPs). Statistical analysis was done using a Kruskal-Wallis ANOVA with a post-hoc Mann-WhitneyU two-tailed test, and Spearman's correlation. Results. We demonstrated transient cognitive impairment alongside a slower alpha fre- quency (αAVG) in the subacute right MCA stroke patients vs. the controls. This slower alpha frequency showed no amplitude change, but was highly synchronized intra- hemispherically, overlying the ipsi-lesional hemisphere, and inter-hemispherically, overlying the frontal cortex. In addition, the disturbances in EEG alpha activity in subacute stroke patients were expressed as a decrease in alpha PPs over the frontal cortex and an altered "alpha flow", indicating the sustained augmentation of inter- hemispheric interactions. Although the stroke induced slower alpha was a transient phenomenon, the increased alpha intra-hemispheric synchronization, overlying the ipsi-lesional hemisphere, the increased alpha F3-F4 inter-hemispheric synchronization, the delayed alpha waves, and the newly established inter-hemispheric "alpha flow" within the frontal cortex, remained as a permanent consequence of the minor stroke. This newly established frontal inter-hemispheric "alpha flow" represented a permanent consequence of the ``hidden" stroke neuropathology, despite the fact that cognitive impairment has been returned to the control values. All the detected permanent changes at the EEG level with no cognitive impairment after a minor stroke could be a way for the brain to compensate for the lesion and restore the lost function. Discussion. Our study indicates slower EEG alpha generation, synchronization and ``flow" as potential biomarkers of cognitive impairment onset and/or compensatory post-stroke re-organizational processes.",
journal = "PeerJ",
title = "Slower EEG alpha generation, synchronization and “flow”—possible biomarkers of cognitive impairment and neuropathology of minor stroke",
number = "9",
volume = "5",
doi = "10.7717/peerj.3839",
pages = "e3839-e3839"
}

Petrović, J., Milosevic, V., Živković, M., Stojanov, D., Milojković, O., Kalauzi, A.,& Šaponjić, J.. (2017). Slower EEG alpha generation, synchronization and “flow”—possible biomarkers of cognitive impairment and neuropathology of minor stroke. in PeerJ, 5(9), e3839-e3839.
https://doi.org/10.7717/peerj.3839

Petrović J, Milosevic V, Živković M, Stojanov D, Milojković O, Kalauzi A, Šaponjić J. Slower EEG alpha generation, synchronization and “flow”—possible biomarkers of cognitive impairment and neuropathology of minor stroke. in PeerJ. 2017;5(9):e3839-e3839.
doi:10.7717/peerj.3839 .

Petrović, Jelena, Milosevic, Vuk, Živković, Miroslava, Stojanov, Dragan, Milojković, Olga, Kalauzi, Aleksandar, Šaponjić, Jasna, "Slower EEG alpha generation, synchronization and “flow”—possible biomarkers of cognitive impairment and neuropathology of minor stroke" in PeerJ, 5, no. 9 (2017):e3839-e3839,
https://doi.org/10.7717/peerj.3839 . .

TY  - JOUR
AU  - Lazić, Katarina
AU  - Petrović, Jelena
AU  - Ćirić, Jelena
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0031938416306308
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3297
AB  - Postoperative sleep disorders, particularly the REM sleep disorder, may have a significant deleterious impact on postoperative outcomes and may contribute to the genesis of certain delayed postoperative complications. We have followed the effect of distinct anesthesia regimens (ketamine/diazepam vs. pentobarbital) over 6days following the induction of a stable anesthetized state in adult male Wistar rats, chronically instrumented for sleep recording. In order to compare the effect of both anesthetics in the physiological controls vs. the rats with impaired pedunculopontine tegmental nucleus (PPT) cholinergic innervation, during the operative procedure for the implantation of EEG and EMG electrodes, the bilateral PPT lesion was conducted using ibotenic acid (IBO). We have followed in particular post-anesthesia REM sleep. Our results show the distinct EEG microstructure of the motor cortex during the different stable anesthetized states, and their distinct impact on post-anesthesia REM sleep. In contrast to pentobarbital anesthesia, the ketamine/diazepam anesthesia potentiated the long-lasting post-anesthesia REM statewith higher muscle tone (REM1) vs. REM state with atonia (REM2). Whereas both anesthesias prolonged the post-anesthesia REM sleep duration, the long-term prolongation of the REM1 state was demonstrated only after the ketamine/diazepam anesthesia, first due to the increased number of REM1 episodes, and then due to the prolonged REM1 episodes duration. On the other hand, whereas both anesthetic regimens abolished the prolonged post-anesthesia REM/REM1 sleep and the EEG microstructure disorder during REM sleep, only the pentobarbital abolished the increased NREM/REM/NREM transitions, caused by the PPT lesion. In addition, in the PPT lesioned rats, the ketamine/diazepam anesthesia decreased the Wake/NREM/Wake transitions while the pentobarbital anesthesia decreased the Wake/REM/Wake transitions. Our present study suggests pentobarbital anesthesia as being highly beneficial for post-anesthesia REM sleep in the physiological condition as well as during PPT cholinergic neuropathology.
T2  - Physiology & Behavior
T1  - REM sleep disorder following general anesthesia in rats.
VL  - 168
DO  - 10.1016/j.physbeh.2016.10.013
SP  - 41
EP  - 54
ER  - 

@article{
author = "Lazić, Katarina and Petrović, Jelena and Ćirić, Jelena and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2017",
abstract = "Postoperative sleep disorders, particularly the REM sleep disorder, may have a significant deleterious impact on postoperative outcomes and may contribute to the genesis of certain delayed postoperative complications. We have followed the effect of distinct anesthesia regimens (ketamine/diazepam vs. pentobarbital) over 6days following the induction of a stable anesthetized state in adult male Wistar rats, chronically instrumented for sleep recording. In order to compare the effect of both anesthetics in the physiological controls vs. the rats with impaired pedunculopontine tegmental nucleus (PPT) cholinergic innervation, during the operative procedure for the implantation of EEG and EMG electrodes, the bilateral PPT lesion was conducted using ibotenic acid (IBO). We have followed in particular post-anesthesia REM sleep. Our results show the distinct EEG microstructure of the motor cortex during the different stable anesthetized states, and their distinct impact on post-anesthesia REM sleep. In contrast to pentobarbital anesthesia, the ketamine/diazepam anesthesia potentiated the long-lasting post-anesthesia REM statewith higher muscle tone (REM1) vs. REM state with atonia (REM2). Whereas both anesthesias prolonged the post-anesthesia REM sleep duration, the long-term prolongation of the REM1 state was demonstrated only after the ketamine/diazepam anesthesia, first due to the increased number of REM1 episodes, and then due to the prolonged REM1 episodes duration. On the other hand, whereas both anesthetic regimens abolished the prolonged post-anesthesia REM/REM1 sleep and the EEG microstructure disorder during REM sleep, only the pentobarbital abolished the increased NREM/REM/NREM transitions, caused by the PPT lesion. In addition, in the PPT lesioned rats, the ketamine/diazepam anesthesia decreased the Wake/NREM/Wake transitions while the pentobarbital anesthesia decreased the Wake/REM/Wake transitions. Our present study suggests pentobarbital anesthesia as being highly beneficial for post-anesthesia REM sleep in the physiological condition as well as during PPT cholinergic neuropathology.",
journal = "Physiology & Behavior",
title = "REM sleep disorder following general anesthesia in rats.",
volume = "168",
doi = "10.1016/j.physbeh.2016.10.013",
pages = "41-54"
}

Lazić, K., Petrović, J., Ćirić, J., Kalauzi, A.,& Šaponjić, J.. (2017). REM sleep disorder following general anesthesia in rats.. in Physiology & Behavior, 168, 41-54.
https://doi.org/10.1016/j.physbeh.2016.10.013

Lazić K, Petrović J, Ćirić J, Kalauzi A, Šaponjić J. REM sleep disorder following general anesthesia in rats.. in Physiology & Behavior. 2017;168:41-54.
doi:10.1016/j.physbeh.2016.10.013 .

Lazić, Katarina, Petrović, Jelena, Ćirić, Jelena, Kalauzi, Aleksandar, Šaponjić, Jasna, "REM sleep disorder following general anesthesia in rats." in Physiology & Behavior, 168 (2017):41-54,
https://doi.org/10.1016/j.physbeh.2016.10.013 . .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
AU  - Petrović, Jelena
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1909
UR  - http://www.sciencedirect.com/science/article/pii/S0166432815303454
AB  - We studied the impact of aging during sleep in the rat models of
   Alzheimer's (AD) and Parkinson's (PD) disease cholinergic neuropathology
   to determine the possible different and earlier onset of age-related
   sleep disorder during the neurodegenerative diseases vs. healthy aging.
   We used the bilateral nucleus basalis (NB) and pedunculopontine
   tegmental nucleus (PPT) lesioned rats as the in vivo models of
   functionally distinct cholinergic neuropathology, and we followed the
   impact of aging on sleep architecture, the electroencephalographic (EEG)
   microstructure and motor control across sleep/wake states.
   Our results have shown for the first time that the earliest signs of
   aging during distinct cholinergic neuropathology were expressed through
   a different and topographically specific EEG microstructure during rapid
   eye movement sleep (REM). EEG delta amplitude attenuation within the
   sensorimotor cortex (SMCx) during REM was the earliest sign of aging in
   the NB lesion. EEG sigma amplitude augmentation within the motor cortex
   (MCx) during REM was the earliest sign of aging in the PPT lesion. In
   addition, aging was differently expressed through the SMCx drive
   alterations, but it was commonly expressed through the MCx drive
   alterations during all sleep/wake states.
   Our study provided evidence of distinct REM sleep disorders and sleep
   state related cortical drives as the signs of aging onset during
   functionally distinct cholinergic neuropathologies (NB lesion vs. PPT
   lesion). (C) 2015 Elsevier B.V. All rights reserved.
T2  - Behavioural Brain Research
T1  - Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology
VL  - 301
DO  - 10.1016/j.bbr.2015.12.046
SP  - 273
EP  - 286
ER  - 

@article{
author = "Ćirić, Jelena and Lazić, Katarina and Petrović, Jelena and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2016",
abstract = "We studied the impact of aging during sleep in the rat models of
   Alzheimer's (AD) and Parkinson's (PD) disease cholinergic neuropathology
   to determine the possible different and earlier onset of age-related
   sleep disorder during the neurodegenerative diseases vs. healthy aging.
   We used the bilateral nucleus basalis (NB) and pedunculopontine
   tegmental nucleus (PPT) lesioned rats as the in vivo models of
   functionally distinct cholinergic neuropathology, and we followed the
   impact of aging on sleep architecture, the electroencephalographic (EEG)
   microstructure and motor control across sleep/wake states.
   Our results have shown for the first time that the earliest signs of
   aging during distinct cholinergic neuropathology were expressed through
   a different and topographically specific EEG microstructure during rapid
   eye movement sleep (REM). EEG delta amplitude attenuation within the
   sensorimotor cortex (SMCx) during REM was the earliest sign of aging in
   the NB lesion. EEG sigma amplitude augmentation within the motor cortex
   (MCx) during REM was the earliest sign of aging in the PPT lesion. In
   addition, aging was differently expressed through the SMCx drive
   alterations, but it was commonly expressed through the MCx drive
   alterations during all sleep/wake states.
   Our study provided evidence of distinct REM sleep disorders and sleep
   state related cortical drives as the signs of aging onset during
   functionally distinct cholinergic neuropathologies (NB lesion vs. PPT
   lesion). (C) 2015 Elsevier B.V. All rights reserved.",
journal = "Behavioural Brain Research",
title = "Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology",
volume = "301",
doi = "10.1016/j.bbr.2015.12.046",
pages = "273-286"
}

Ćirić, J., Lazić, K., Petrović, J., Kalauzi, A.,& Šaponjić, J.. (2016). Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology. in Behavioural Brain Research, 301, 273-286.
https://doi.org/10.1016/j.bbr.2015.12.046

Ćirić J, Lazić K, Petrović J, Kalauzi A, Šaponjić J. Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology. in Behavioural Brain Research. 2016;301:273-286.
doi:10.1016/j.bbr.2015.12.046 .

Ćirić, Jelena, Lazić, Katarina, Petrović, Jelena, Kalauzi, Aleksandar, Šaponjić, Jasna, "Age-related disorders of sleep and motor control in the rat models of
 functionally distinct cholinergic neuropathology" in Behavioural Brain Research, 301 (2016):273-286,
https://doi.org/10.1016/j.bbr.2015.12.046 . .

TY  - JOUR
AU  - Kalauzi, Aleksandar
AU  - Nikolić, Ljiljana
AU  - Savić, Danijela
AU  - Radotic, Ksenija
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2011
AB  - The physiological and biochemical factors that lead to cell death have
   not been recognized completely. To our knowledge, there are no data on
   the bioelectric parameters that characterize early period of cell death,
   as well as on the appearance of related membrane current frequencies. We
   studied early parameters of glutaraldehyde (GA)-induced cell death, by
   examining the membrane properties of mouse microglia using the
   whole-cell patch-clamp technique. In addition, we investigated the
   GA-induced changes in the membrane current frequency, to see if
   characteristic frequencies would appear in dying cell. For data
   analysis, we applied a new approach, an improved multiple moving window
   length analysis and interval weighted spectra averaging (IWSA). We chose
   GA for its ability to induce almost instantaneous cell death. The 0.6 \%
   GA did not induce changes in the bioelectric membrane properties of
   microglia. However, the 3 \% GA caused significant decrease of membrane
   capacitance and resistance accompanied by the prominent increase in the
   membrane currents and nearly ohmic current response of microglial cells.
   These data indicate that 3 \% GA caused complete loss of the membrane
   function consequently inducing instantaneous cell death. The membrane
   function loss was characterized by appearance of the 1.26-4.62 Hz
   frequency peak in the IWSA spectra, while no significant increase of
   amplitudes could be observed for cells treated with 0.6 \% GA. To our
   knowledge, this is the first record of a frequency associated with
   complete loss of the membrane function and thus can be considered as an
   early indicator of cell death.
T2  - Journal of Membrane Biology
T1  - Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval
 Weighted Spectra Averaging: Changes in Membrane Properties and Current
 Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde
IS  - 1
VL  - 248
DO  - 10.1007/s00232-014-9748-7
SP  - 117
EP  - 123
ER  - 

@article{
author = "Kalauzi, Aleksandar and Nikolić, Ljiljana and Savić, Danijela and Radotic, Ksenija",
year = "2015",
abstract = "The physiological and biochemical factors that lead to cell death have
   not been recognized completely. To our knowledge, there are no data on
   the bioelectric parameters that characterize early period of cell death,
   as well as on the appearance of related membrane current frequencies. We
   studied early parameters of glutaraldehyde (GA)-induced cell death, by
   examining the membrane properties of mouse microglia using the
   whole-cell patch-clamp technique. In addition, we investigated the
   GA-induced changes in the membrane current frequency, to see if
   characteristic frequencies would appear in dying cell. For data
   analysis, we applied a new approach, an improved multiple moving window
   length analysis and interval weighted spectra averaging (IWSA). We chose
   GA for its ability to induce almost instantaneous cell death. The 0.6 \%
   GA did not induce changes in the bioelectric membrane properties of
   microglia. However, the 3 \% GA caused significant decrease of membrane
   capacitance and resistance accompanied by the prominent increase in the
   membrane currents and nearly ohmic current response of microglial cells.
   These data indicate that 3 \% GA caused complete loss of the membrane
   function consequently inducing instantaneous cell death. The membrane
   function loss was characterized by appearance of the 1.26-4.62 Hz
   frequency peak in the IWSA spectra, while no significant increase of
   amplitudes could be observed for cells treated with 0.6 \% GA. To our
   knowledge, this is the first record of a frequency associated with
   complete loss of the membrane function and thus can be considered as an
   early indicator of cell death.",
journal = "Journal of Membrane Biology",
title = "Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval
 Weighted Spectra Averaging: Changes in Membrane Properties and Current
 Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde",
number = "1",
volume = "248",
doi = "10.1007/s00232-014-9748-7",
pages = "117-123"
}

Kalauzi, A., Nikolić, L., Savić, D.,& Radotic, K.. (2015). Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval
 Weighted Spectra Averaging: Changes in Membrane Properties and Current
 Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde. in Journal of Membrane Biology, 248(1), 117-123.
https://doi.org/10.1007/s00232-014-9748-7

Kalauzi A, Nikolić L, Savić D, Radotic K. Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval
 Weighted Spectra Averaging: Changes in Membrane Properties and Current
 Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde. in Journal of Membrane Biology. 2015;248(1):117-123.
doi:10.1007/s00232-014-9748-7 .

Kalauzi, Aleksandar, Nikolić, Ljiljana, Savić, Danijela, Radotic, Ksenija, "Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval
 Weighted Spectra Averaging: Changes in Membrane Properties and Current
 Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde" in Journal of Membrane Biology, 248, no. 1 (2015):117-123,
https://doi.org/10.1007/s00232-014-9748-7 . .

TY  - JOUR
AU  - Pešić, Milica
AU  - Podolski-Renić, Ana
AU  - Stojković Burić, Sonja
AU  - Matovic, Branko
AU  - Zmejkoski, Danica
AU  - Kojic, Vesna
AU  - Bogdanovic, Gordana
AU  - Pavicevic, Aleksandra
AU  - Mojovic, Milos
AU  - Savic, Aleksandar
AU  - Milenkovic, Ivana
AU  - Kalauzi, Aleksandar
AU  - Radotic, Ksenija
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1957
AB  - Data on medical applications of cerium oxide nanoparticles CeO2 (CONP)
   are promising, yet information regarding their action in cells is
   incomplete and there are conflicting reports about in vitro toxicity.
   Herein, we have studied cytotoxic effect of CONP in several cancer and
   normal cell lines and their potential to change intracellular redox
   status. The IC50 was achieved only in two of eight tested cell lines,
   melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating
   room temperature method was applied to produce CONP with an average
   crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2-
   vacancies. The induction of cell death by CONP and the production of
   reactive oxygen species (ROS) were analyzed by flow-cytometry. Free
   radicals related antioxidant capacity of the cells was studied by the
   reduction of stable free radical TEMPONE using electron spin resonance
   spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell
   lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small
   cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while
   normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5)
   were insensitive. The most sensitive were 518A2 melanoma and HT-29
   colorectal adenocarcinoma cell lines, with the IC50 values being between
   100 and 200 mu M. Decreased rate of TEMPONE reduction and increased
   production of certain ROS species (peroxynitrite and hydrogen peroxide
   anion) indicates that free radical metabolism, thus redox status was
   changed, and antioxidant capacity damaged in the CONP treated 518A2 and
   HT-29 cells. In conclusion, changes in intracellular redox status
   induced by CONP are partly attributed to the prooxidant activity of the
   nanoparticles. Further, ROS induced cell damages might eventually lead
   to the cell death. However, low inhibitory potential of CONP in the
   other human cell lines tested indicates that CONP may be safe for human
   usage in industry and medicine. (C) 2015 Elsevier Ireland Ltd. All
   rights reserved.
T2  - Chemico-Biological Interactions
T1  - Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity
VL  - 232
DO  - 10.1016/j.cbi.2015.03.013
SP  - 85
EP  - 93
ER  - 

@article{
author = "Pešić, Milica and Podolski-Renić, Ana and Stojković Burić, Sonja and Matovic, Branko and Zmejkoski, Danica and Kojic, Vesna and Bogdanovic, Gordana and Pavicevic, Aleksandra and Mojovic, Milos and Savic, Aleksandar and Milenkovic, Ivana and Kalauzi, Aleksandar and Radotic, Ksenija",
year = "2015",
abstract = "Data on medical applications of cerium oxide nanoparticles CeO2 (CONP)
   are promising, yet information regarding their action in cells is
   incomplete and there are conflicting reports about in vitro toxicity.
   Herein, we have studied cytotoxic effect of CONP in several cancer and
   normal cell lines and their potential to change intracellular redox
   status. The IC50 was achieved only in two of eight tested cell lines,
   melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating
   room temperature method was applied to produce CONP with an average
   crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2-
   vacancies. The induction of cell death by CONP and the production of
   reactive oxygen species (ROS) were analyzed by flow-cytometry. Free
   radicals related antioxidant capacity of the cells was studied by the
   reduction of stable free radical TEMPONE using electron spin resonance
   spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell
   lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small
   cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while
   normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5)
   were insensitive. The most sensitive were 518A2 melanoma and HT-29
   colorectal adenocarcinoma cell lines, with the IC50 values being between
   100 and 200 mu M. Decreased rate of TEMPONE reduction and increased
   production of certain ROS species (peroxynitrite and hydrogen peroxide
   anion) indicates that free radical metabolism, thus redox status was
   changed, and antioxidant capacity damaged in the CONP treated 518A2 and
   HT-29 cells. In conclusion, changes in intracellular redox status
   induced by CONP are partly attributed to the prooxidant activity of the
   nanoparticles. Further, ROS induced cell damages might eventually lead
   to the cell death. However, low inhibitory potential of CONP in the
   other human cell lines tested indicates that CONP may be safe for human
   usage in industry and medicine. (C) 2015 Elsevier Ireland Ltd. All
   rights reserved.",
journal = "Chemico-Biological Interactions",
title = "Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity",
volume = "232",
doi = "10.1016/j.cbi.2015.03.013",
pages = "85-93"
}

Pešić, M., Podolski-Renić, A., Stojković Burić, S., Matovic, B., Zmejkoski, D., Kojic, V., Bogdanovic, G., Pavicevic, A., Mojovic, M., Savic, A., Milenkovic, I., Kalauzi, A.,& Radotic, K.. (2015). Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity. in Chemico-Biological Interactions, 232, 85-93.
https://doi.org/10.1016/j.cbi.2015.03.013

Pešić M, Podolski-Renić A, Stojković Burić S, Matovic B, Zmejkoski D, Kojic V, Bogdanovic G, Pavicevic A, Mojovic M, Savic A, Milenkovic I, Kalauzi A, Radotic K. Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity. in Chemico-Biological Interactions. 2015;232:85-93.
doi:10.1016/j.cbi.2015.03.013 .

Pešić, Milica, Podolski-Renić, Ana, Stojković Burić, Sonja, Matovic, Branko, Zmejkoski, Danica, Kojic, Vesna, Bogdanovic, Gordana, Pavicevic, Aleksandra, Mojovic, Milos, Savic, Aleksandar, Milenkovic, Ivana, Kalauzi, Aleksandar, Radotic, Ksenija, "Anti-cancer effects of cerium oxide nanoparticles and its intracellular
 redox activity" in Chemico-Biological Interactions, 232 (2015):85-93,
https://doi.org/10.1016/j.cbi.2015.03.013 . .

TY  - JOUR
AU  - Ciric, Jelena
AU  - Lazic, Katarina
AU  - Petrovic, Jelena
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1990
AB  - We followed the impact of healthy aging on cortical drive during sleep
   in rats by using the corticomuscular coherence (CMC).
   We employed the chronic electrodes implantation for sleep recording in
   adult, male Wistar rats, and followed the aging impact during sleep from
   3 to 5.5 months age. We have analyzed the sleep/wake states
   architecture, and the sleep/wake state related EEG microstructure and
   CMCs.
   We evidenced the topographically distinct impact of aging on sleep/wake
   states architecture within the sensorimotor (SMCx) vs. motor cortex
   (MCx) from 4.5 to 5.5 months age. Healthy aging consistently altered
   only the SMCx sleep/wake states architecture, and increased the delta
   and beta CMCs through both cortical drives during Wake, but only through
   the MCx drive during REM. According to the delta and beta CMCs values,
   aging impact through the SMCx drive was opposite, but it was convergent
   through the MCx drive during Wake vs. REM, and there was a dual and
   inverse mode for the motor control during REM. (C) 2015 Elsevier Ireland
   Ltd. All rights reserved.
T2  - Mechanisms of Ageing and Development
T1  - Aging induced cortical drive alterations during sleep in rats
VL  - 146
DO  - 10.1016/j.mad.2015.03.002
SP  - 12
EP  - 22
ER  - 

@article{
author = "Ciric, Jelena and Lazic, Katarina and Petrovic, Jelena and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2015",
abstract = "We followed the impact of healthy aging on cortical drive during sleep
   in rats by using the corticomuscular coherence (CMC).
   We employed the chronic electrodes implantation for sleep recording in
   adult, male Wistar rats, and followed the aging impact during sleep from
   3 to 5.5 months age. We have analyzed the sleep/wake states
   architecture, and the sleep/wake state related EEG microstructure and
   CMCs.
   We evidenced the topographically distinct impact of aging on sleep/wake
   states architecture within the sensorimotor (SMCx) vs. motor cortex
   (MCx) from 4.5 to 5.5 months age. Healthy aging consistently altered
   only the SMCx sleep/wake states architecture, and increased the delta
   and beta CMCs through both cortical drives during Wake, but only through
   the MCx drive during REM. According to the delta and beta CMCs values,
   aging impact through the SMCx drive was opposite, but it was convergent
   through the MCx drive during Wake vs. REM, and there was a dual and
   inverse mode for the motor control during REM. (C) 2015 Elsevier Ireland
   Ltd. All rights reserved.",
journal = "Mechanisms of Ageing and Development",
title = "Aging induced cortical drive alterations during sleep in rats",
volume = "146",
doi = "10.1016/j.mad.2015.03.002",
pages = "12-22"
}

Ciric, J., Lazic, K., Petrovic, J., Kalauzi, A.,& Šaponjić, J.. (2015). Aging induced cortical drive alterations during sleep in rats. in Mechanisms of Ageing and Development, 146, 12-22.
https://doi.org/10.1016/j.mad.2015.03.002

Ciric J, Lazic K, Petrovic J, Kalauzi A, Šaponjić J. Aging induced cortical drive alterations during sleep in rats. in Mechanisms of Ageing and Development. 2015;146:12-22.
doi:10.1016/j.mad.2015.03.002 .

Ciric, Jelena, Lazic, Katarina, Petrovic, Jelena, Kalauzi, Aleksandar, Šaponjić, Jasna, "Aging induced cortical drive alterations during sleep in rats" in Mechanisms of Ageing and Development, 146 (2015):12-22,
https://doi.org/10.1016/j.mad.2015.03.002 . .

TY  - JOUR
AU  - Petrovic, Jelena
AU  - Lazic, Katarina
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2169
AB  - The aim of this study was to demonstrate that two REM clusters, which
   emerge following bilateral pedunculopontine tegmental nucleus (PPT)
   lesions in rats, are two functionally distinct REM states.
   We performed the experiments in Wistar rats, chronically instrumented
   for sleep recording. Bilateral PPT lesions were produced by the
   microinfusion of 100 nl of 0.1 M ibotenic acid (IBO). Following a
   recovery period of 2 weeks, we recorded their sleep for 6 h. Bilateral
   PPT lesions were identified by NADPH diaphorase histochemistry.
   We applied Fourier analysis to the signals acquired throughout the 6 h
   recordings, and each 10 s epoch was differentiated as a Wake, NREM or
   REM state. We analyzed the topography of the sleep/wake states
   architecture and their transition structure, their all state-related EEG
   microstructures, and the sensorimotor (SMCx) and motor (MCx) cortex REM
   related cortico-muscular coherences (CMCs).
   Bilateral PPT lesion in rats increased the likelihood of the emergence
   of two distinct REM sleep states, specifically expressed within the MCx:
   REM1 and REM2. Bilateral PPT lesion did not change the sleep/wake states
   architecture of the SMCx, but pathologically increased the duration of
   REM1 within the MCx, alongside increasing Wake/REM1/Wake and
   NREM/REM2/NREM transitions within both cortices. In addition, the
   augmented total REM SMCx EEG beta amplitude and REM1 MCx EEG theta
   amplitude was the underlying EEG microstructure pathology.
   PPT lesion induced REM1 and REM2 are differential states with regard to
   total EMG power, topographically distinct EEG microstructures, and
   locomotor drives to nuchal musculature. (C) 2014 Elsevier B.V. All
   rights reserved.
T2  - Behavioural Brain Research
T1  - REM sleep diversity following the pedunculopontine tegmental nucleus
 lesion in rat
VL  - 271
DO  - 10.1016/j.bbr.2014.06.026
SP  - 258
EP  - 268
ER  - 

@article{
author = "Petrovic, Jelena and Lazic, Katarina and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2014",
abstract = "The aim of this study was to demonstrate that two REM clusters, which
   emerge following bilateral pedunculopontine tegmental nucleus (PPT)
   lesions in rats, are two functionally distinct REM states.
   We performed the experiments in Wistar rats, chronically instrumented
   for sleep recording. Bilateral PPT lesions were produced by the
   microinfusion of 100 nl of 0.1 M ibotenic acid (IBO). Following a
   recovery period of 2 weeks, we recorded their sleep for 6 h. Bilateral
   PPT lesions were identified by NADPH diaphorase histochemistry.
   We applied Fourier analysis to the signals acquired throughout the 6 h
   recordings, and each 10 s epoch was differentiated as a Wake, NREM or
   REM state. We analyzed the topography of the sleep/wake states
   architecture and their transition structure, their all state-related EEG
   microstructures, and the sensorimotor (SMCx) and motor (MCx) cortex REM
   related cortico-muscular coherences (CMCs).
   Bilateral PPT lesion in rats increased the likelihood of the emergence
   of two distinct REM sleep states, specifically expressed within the MCx:
   REM1 and REM2. Bilateral PPT lesion did not change the sleep/wake states
   architecture of the SMCx, but pathologically increased the duration of
   REM1 within the MCx, alongside increasing Wake/REM1/Wake and
   NREM/REM2/NREM transitions within both cortices. In addition, the
   augmented total REM SMCx EEG beta amplitude and REM1 MCx EEG theta
   amplitude was the underlying EEG microstructure pathology.
   PPT lesion induced REM1 and REM2 are differential states with regard to
   total EMG power, topographically distinct EEG microstructures, and
   locomotor drives to nuchal musculature. (C) 2014 Elsevier B.V. All
   rights reserved.",
journal = "Behavioural Brain Research",
title = "REM sleep diversity following the pedunculopontine tegmental nucleus
 lesion in rat",
volume = "271",
doi = "10.1016/j.bbr.2014.06.026",
pages = "258-268"
}

Petrovic, J., Lazic, K., Kalauzi, A.,& Šaponjić, J.. (2014). REM sleep diversity following the pedunculopontine tegmental nucleus
 lesion in rat. in Behavioural Brain Research, 271, 258-268.
https://doi.org/10.1016/j.bbr.2014.06.026

Petrovic J, Lazic K, Kalauzi A, Šaponjić J. REM sleep diversity following the pedunculopontine tegmental nucleus
 lesion in rat. in Behavioural Brain Research. 2014;271:258-268.
doi:10.1016/j.bbr.2014.06.026 .

Petrovic, Jelena, Lazic, Katarina, Kalauzi, Aleksandar, Šaponjić, Jasna, "REM sleep diversity following the pedunculopontine tegmental nucleus
 lesion in rat" in Behavioural Brain Research, 271 (2014):258-268,
https://doi.org/10.1016/j.bbr.2014.06.026 . .

TY  - JOUR
AU  - Šaponjić, Jasna
AU  - Petrović, Jelena
AU  - Kalauzi, Aleksandar
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
AU  - Radulovacki, Miodrag
AU  - Carley, David W
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1027
AB  - We examined the effects of unilateral and bilateral nucleus basalis (NB) lesion in rat on sleep/wake states, and sleep/wake state-related electroencephalographic (EEG) frequency relative amplitude distributions. We aimed this study to identify the possible EEG markers for the onset and progression of cortical cholinergic neurodegeneration in rats. NB lesion was performed by ibotenic acid (IBO) microinfusion, and identified by NADPH-diaphorase histochemistry. Sleep/wake states related EEG relative amplitude analysis was done using the Probability Density Estimate (PDE) routine supplied with MATLAB 6.5. Bilateral NB lesion transiently altered gross sleep/wake states topography 14 days following lesion. While control rats exhibited equivalent durations of Wake, NREM and REM, as determined by sensorimotor versus motor cortex EEG, bilateral NB lesion decreased Wake duration in both cortices, with NREM duration increased within sensorimotor cortex, and REM duration increased within motor cortex. Also, Wake, NREM and REM theta relative amplitude was lower in motor versus sensorimotor cortex in all groups of animals. In sensorimotor cortex bilateral NB lesion increased only REM theta relative amplitude from 1421 days following lesion, and returned to control value 28 days following lesion. In motor cortex both Wake and REM theta relative amplitude transiently increased 14 days following unilateral and bilateral NB lesion, and returned to control values 21 days after lesions. We demonstrated at functional level, for the first time, the topographically specific impact of NB cholinergic cortical afferent system dysregulation on sleep/wake states, REM and Wake EEG theta relative amplitude.
T2  - Sleep and Biological Rhythms
T1  - Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder
IS  - 2
VL  - 11
SP  - 243
EP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1027
ER  - 

@article{
author = "Šaponjić, Jasna and Petrović, Jelena and Kalauzi, Aleksandar and Ćirić, Jelena and Lazić, Katarina and Radulovacki, Miodrag and Carley, David W",
year = "2013",
abstract = "We examined the effects of unilateral and bilateral nucleus basalis (NB) lesion in rat on sleep/wake states, and sleep/wake state-related electroencephalographic (EEG) frequency relative amplitude distributions. We aimed this study to identify the possible EEG markers for the onset and progression of cortical cholinergic neurodegeneration in rats. NB lesion was performed by ibotenic acid (IBO) microinfusion, and identified by NADPH-diaphorase histochemistry. Sleep/wake states related EEG relative amplitude analysis was done using the Probability Density Estimate (PDE) routine supplied with MATLAB 6.5. Bilateral NB lesion transiently altered gross sleep/wake states topography 14 days following lesion. While control rats exhibited equivalent durations of Wake, NREM and REM, as determined by sensorimotor versus motor cortex EEG, bilateral NB lesion decreased Wake duration in both cortices, with NREM duration increased within sensorimotor cortex, and REM duration increased within motor cortex. Also, Wake, NREM and REM theta relative amplitude was lower in motor versus sensorimotor cortex in all groups of animals. In sensorimotor cortex bilateral NB lesion increased only REM theta relative amplitude from 1421 days following lesion, and returned to control value 28 days following lesion. In motor cortex both Wake and REM theta relative amplitude transiently increased 14 days following unilateral and bilateral NB lesion, and returned to control values 21 days after lesions. We demonstrated at functional level, for the first time, the topographically specific impact of NB cholinergic cortical afferent system dysregulation on sleep/wake states, REM and Wake EEG theta relative amplitude.",
journal = "Sleep and Biological Rhythms",
title = "Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder",
number = "2",
volume = "11",
pages = "243-115",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1027"
}

Šaponjić, J., Petrović, J., Kalauzi, A., Ćirić, J., Lazić, K., Radulovacki, M.,& Carley, D. W.. (2013). Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder. in Sleep and Biological Rhythms, 11(2), 243-115.
https://hdl.handle.net/21.15107/rcub_ibiss_1027

Šaponjić J, Petrović J, Kalauzi A, Ćirić J, Lazić K, Radulovacki M, Carley DW. Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder. in Sleep and Biological Rhythms. 2013;11(2):243-115.
https://hdl.handle.net/21.15107/rcub_ibiss_1027 .

Šaponjić, Jasna, Petrović, Jelena, Kalauzi, Aleksandar, Ćirić, Jelena, Lazić, Katarina, Radulovacki, Miodrag, Carley, David W, "Sleep-state related EEG amplitude distribution in the rat model of cortical cholinergic innervation disorder" in Sleep and Biological Rhythms, 11, no. 2 (2013):243-115,
https://hdl.handle.net/21.15107/rcub_ibiss_1027 .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Lazić, Katarina
AU  - Ćirić, Jelena
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/949
AB  - In order to identify the differences for the onset and progression of functionally distinct cholinergic innervation disorders, we investigated the effect of bilateral nucleus basalis (NB) and pedunculopontine tegmental nucleus (PPT) lesions on sleep/wake states and electroencephalographic (EEG) microstructure in rats, chronically implanted for sleep recording. Bilateral NB lesion transiently altered Wake/NREM duration within the sensorimotor cortex, and Wake/REM duration within the motor cortex, while there was no change in the sleep/wake states distributions following the bilateral PPT lesion. Bilateral PPT lesion sustainably increased the Wake/REM and REM/Wake transitions followed by inconsistent dysregulation of the NREM/REM and REM/NREM transitions in sensorimotor cortex, but oppositely by their increment throughout four weeks in motor cortex. Bilateral NB lesion sustainably decreased the NREM/REM and REM/NREM transitions during four weeks in the sensorimotor cortex, but oppositely increased them in the motor cortex. We have shown that the sustained beta and gamma augmentation within the sensorimotor and motor cortex, and across all sleep/wake states, simultaneously with Wake delta amplitude attenuation only within the sensorimotor cortex, were the underlying EEG microstructure for the sleep/wake states-transitions structure disturbance following bilateral PPT lesion. In contrast, the bilateral NB lesion only augmented REM theta in sensorimotor cortex during three weeks. We have shown that the NB and PPT lesions induced differing, structure-related EEG microstructure and transition structure disturbances particularly expressed in motor cortex during NREM and REM sleep. We evidenced for the first time the different topographical expression of the functionally distinct cholinergic neuronal innervation impairment in rat. (C) 2013 Elsevier B.V. All rights reserved.
T2  - Behavioural Brain Research
T1  - Topography of the sleep/wake states related EEG microstructure and transitions structure differentiates the functionally distinct cholinergic innervation disorders in rat
IS  - null
VL  - 256
SP  - 41
EP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_949
ER  - 

@article{
author = "Petrović, Jelena and Lazić, Katarina and Ćirić, Jelena and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2013",
abstract = "In order to identify the differences for the onset and progression of functionally distinct cholinergic innervation disorders, we investigated the effect of bilateral nucleus basalis (NB) and pedunculopontine tegmental nucleus (PPT) lesions on sleep/wake states and electroencephalographic (EEG) microstructure in rats, chronically implanted for sleep recording. Bilateral NB lesion transiently altered Wake/NREM duration within the sensorimotor cortex, and Wake/REM duration within the motor cortex, while there was no change in the sleep/wake states distributions following the bilateral PPT lesion. Bilateral PPT lesion sustainably increased the Wake/REM and REM/Wake transitions followed by inconsistent dysregulation of the NREM/REM and REM/NREM transitions in sensorimotor cortex, but oppositely by their increment throughout four weeks in motor cortex. Bilateral NB lesion sustainably decreased the NREM/REM and REM/NREM transitions during four weeks in the sensorimotor cortex, but oppositely increased them in the motor cortex. We have shown that the sustained beta and gamma augmentation within the sensorimotor and motor cortex, and across all sleep/wake states, simultaneously with Wake delta amplitude attenuation only within the sensorimotor cortex, were the underlying EEG microstructure for the sleep/wake states-transitions structure disturbance following bilateral PPT lesion. In contrast, the bilateral NB lesion only augmented REM theta in sensorimotor cortex during three weeks. We have shown that the NB and PPT lesions induced differing, structure-related EEG microstructure and transition structure disturbances particularly expressed in motor cortex during NREM and REM sleep. We evidenced for the first time the different topographical expression of the functionally distinct cholinergic neuronal innervation impairment in rat. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "Behavioural Brain Research",
title = "Topography of the sleep/wake states related EEG microstructure and transitions structure differentiates the functionally distinct cholinergic innervation disorders in rat",
number = "null",
volume = "256",
pages = "41-118",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_949"
}

Petrović, J., Lazić, K., Ćirić, J., Kalauzi, A.,& Šaponjić, J.. (2013). Topography of the sleep/wake states related EEG microstructure and transitions structure differentiates the functionally distinct cholinergic innervation disorders in rat. in Behavioural Brain Research, 256(null), 41-118.
https://hdl.handle.net/21.15107/rcub_ibiss_949

Petrović J, Lazić K, Ćirić J, Kalauzi A, Šaponjić J. Topography of the sleep/wake states related EEG microstructure and transitions structure differentiates the functionally distinct cholinergic innervation disorders in rat. in Behavioural Brain Research. 2013;256(null):41-118.
https://hdl.handle.net/21.15107/rcub_ibiss_949 .

Petrović, Jelena, Lazić, Katarina, Ćirić, Jelena, Kalauzi, Aleksandar, Šaponjić, Jasna, "Topography of the sleep/wake states related EEG microstructure and transitions structure differentiates the functionally distinct cholinergic innervation disorders in rat" in Behavioural Brain Research, 256, no. null (2013):41-118,
https://hdl.handle.net/21.15107/rcub_ibiss_949 .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Ćirić, Jelena
AU  - Lazić, Katarina
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2013
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-84881370170&partnerID=tZOtx3y1
UR  - http://www.sciencedirect.com/science/article/pii/S0014488613000605
UR  - http://www.ncbi.nlm.nih.gov/pubmed/23481548
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3298
AB  - The pedunculopontine tegmental nucleus (PPT) represents a major aggregation of cholinergic neurons in the mammalian brainstem, which is important in the generation and maintenance of REM sleep. We investigated the effects of unilateral and bilateral PPT lesions on sleep and all the conventional sleep-state related EEG frequency bands amplitudes, in an attempt to find the EEG markers for the onset and progression of PPT cholinergic neuronal degeneration. The experiments were performed on 35 adult male Wistar rats, chronically implanted for sleep recording. During the surgical procedure for EEG and EMG electrodes implantation, the unilateral or bilateral PPT lesion was produced under ketamine/diazepam anesthesia, by the stereotaxically guided microinfusion of 100 nl 0.1M ibotenic acid (IBO) into PPT. We applied Fourier analysis to signals acquired throughout 6h of recordings, and each 10s epoch was differentiated as a Wake, NREM or REM state. We also calculated the group probability density estimates (PDE) of all Wake, NREM and REM conventional EEG frequency amplitudes, and the number of all the transition states using MATLAB 6.5. Our results show that the unilateral or bilateral PPT lesions did not change the sleep/wake architecture, but did change the sleep/wake state transitions structure and the sleep/state related "EEG microstructure". Unilateral or bilateral PPT lesions sustainably increased Wake/REM and REM/Wake transitions from 14 to 35 days after lesions. This was followed by decreased NREM/REM and REM/NREM transitions from 28 days only in the case of the bilateral PPT lesion. The unilateral PPT lesion augmented both Wake theta and REM beta while it also attenuated the relative amplitude of the Wake delta frequency, with a delay of one week. Following a bilateral PPT lesion there was augmentation of the relative amplitude of the Wake, NREM, and REM beta and REM gamma frequency which occurred simultaneously to NREM and Wake delta attenuation. We have shown that the PPT cholinergic neuronal loss sustainably increased the number of the Wake/REM and REM/Wake transitions and augmented sleep-states related cortical activation that was simultaneously expressed by the high frequency amplitude augmentation, as well as Wake and NREM delta frequency attenuation.
T2  - Experimental Neurology
T1  - Lesion of the pedunculopontine tegmental nucleus in rat augments cortical activation and disturbs sleep/wake state transitions structure.
VL  - 247
DO  - 10.1016/j.expneurol.2013.02.007
SP  - 562
EP  - 71
ER  - 

@article{
author = "Petrović, Jelena and Ćirić, Jelena and Lazić, Katarina and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2013",
abstract = "The pedunculopontine tegmental nucleus (PPT) represents a major aggregation of cholinergic neurons in the mammalian brainstem, which is important in the generation and maintenance of REM sleep. We investigated the effects of unilateral and bilateral PPT lesions on sleep and all the conventional sleep-state related EEG frequency bands amplitudes, in an attempt to find the EEG markers for the onset and progression of PPT cholinergic neuronal degeneration. The experiments were performed on 35 adult male Wistar rats, chronically implanted for sleep recording. During the surgical procedure for EEG and EMG electrodes implantation, the unilateral or bilateral PPT lesion was produced under ketamine/diazepam anesthesia, by the stereotaxically guided microinfusion of 100 nl 0.1M ibotenic acid (IBO) into PPT. We applied Fourier analysis to signals acquired throughout 6h of recordings, and each 10s epoch was differentiated as a Wake, NREM or REM state. We also calculated the group probability density estimates (PDE) of all Wake, NREM and REM conventional EEG frequency amplitudes, and the number of all the transition states using MATLAB 6.5. Our results show that the unilateral or bilateral PPT lesions did not change the sleep/wake architecture, but did change the sleep/wake state transitions structure and the sleep/state related "EEG microstructure". Unilateral or bilateral PPT lesions sustainably increased Wake/REM and REM/Wake transitions from 14 to 35 days after lesions. This was followed by decreased NREM/REM and REM/NREM transitions from 28 days only in the case of the bilateral PPT lesion. The unilateral PPT lesion augmented both Wake theta and REM beta while it also attenuated the relative amplitude of the Wake delta frequency, with a delay of one week. Following a bilateral PPT lesion there was augmentation of the relative amplitude of the Wake, NREM, and REM beta and REM gamma frequency which occurred simultaneously to NREM and Wake delta attenuation. We have shown that the PPT cholinergic neuronal loss sustainably increased the number of the Wake/REM and REM/Wake transitions and augmented sleep-states related cortical activation that was simultaneously expressed by the high frequency amplitude augmentation, as well as Wake and NREM delta frequency attenuation.",
journal = "Experimental Neurology",
title = "Lesion of the pedunculopontine tegmental nucleus in rat augments cortical activation and disturbs sleep/wake state transitions structure.",
volume = "247",
doi = "10.1016/j.expneurol.2013.02.007",
pages = "562-71"
}

Petrović, J., Ćirić, J., Lazić, K., Kalauzi, A.,& Šaponjić, J.. (2013). Lesion of the pedunculopontine tegmental nucleus in rat augments cortical activation and disturbs sleep/wake state transitions structure.. in Experimental Neurology, 247, 562-71.
https://doi.org/10.1016/j.expneurol.2013.02.007

Petrović J, Ćirić J, Lazić K, Kalauzi A, Šaponjić J. Lesion of the pedunculopontine tegmental nucleus in rat augments cortical activation and disturbs sleep/wake state transitions structure.. in Experimental Neurology. 2013;247:562-71.
doi:10.1016/j.expneurol.2013.02.007 .

Petrović, Jelena, Ćirić, Jelena, Lazić, Katarina, Kalauzi, Aleksandar, Šaponjić, Jasna, "Lesion of the pedunculopontine tegmental nucleus in rat augments cortical activation and disturbs sleep/wake state transitions structure." in Experimental Neurology, 247 (2013):562-71,
https://doi.org/10.1016/j.expneurol.2013.02.007 . .

TY  - JOUR
AU  - Nikolić, Ljiljana
AU  - Todorović, Nataša
AU  - Zakrzewska, Joanna S
AU  - Stanić, Marina B
AU  - Rauš Balind, Snežana
AU  - Kalauzi, Aleksandar
AU  - Petković, Branka
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1168
UR  - https://link.springer.com/article/10.1007/s00359-012-0727-0
AB  - The spontaneously active Br neuron from the brain-subesophageal ganglion complex of the garden snail Helix pomatia rhythmically generates regular bursts of action potentials with quiescent intervals accompanied by slow oscillations of membrane potential. We examined the involvement of the Na+/K+ pump in modulating its bursting activity by applying a static magnetic field. Whole snail brains and Br neuron were exposed to the 10-mT static magnetic field for 15 min. Biochemical data showed that Na+/K+-ATPase activity increased almost twofold after exposure of snail brains to the static magnetic field. Similarly, P-31 NMR data revealed a trend of increasing ATP consumption and increase in intracellular pH mediated by the Na+/H+ exchanger in snail brains exposed to the static magnetic field. Importantly, current clamp recordings from the Br neuron confirmed the increase in activity of the Na+/K+ pump after exposure to the static magnetic field, as the magnitude of ouabain's effect measured on the membrane resting potential, action potential, and interspike interval duration was higher in neurons exposed to the magnetic field. Metabolic pathways through which the magnetic field influenced the Na+/K+ pump could involve phosphorylation and dephosphorylation, as blocking these processes abolished the effect of the static magnetic field.
PB  - Springer
T2  - Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology
T1  - Involvement of Na+/K+ pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field
IS  - 7
VL  - 198
DO  - 10.1007/s00359-012-0727-0
SP  - 525
EP  - 540
ER  - 

@article{
author = "Nikolić, Ljiljana and Todorović, Nataša and Zakrzewska, Joanna S and Stanić, Marina B and Rauš Balind, Snežana and Kalauzi, Aleksandar and Petković, Branka",
year = "2012",
abstract = "The spontaneously active Br neuron from the brain-subesophageal ganglion complex of the garden snail Helix pomatia rhythmically generates regular bursts of action potentials with quiescent intervals accompanied by slow oscillations of membrane potential. We examined the involvement of the Na+/K+ pump in modulating its bursting activity by applying a static magnetic field. Whole snail brains and Br neuron were exposed to the 10-mT static magnetic field for 15 min. Biochemical data showed that Na+/K+-ATPase activity increased almost twofold after exposure of snail brains to the static magnetic field. Similarly, P-31 NMR data revealed a trend of increasing ATP consumption and increase in intracellular pH mediated by the Na+/H+ exchanger in snail brains exposed to the static magnetic field. Importantly, current clamp recordings from the Br neuron confirmed the increase in activity of the Na+/K+ pump after exposure to the static magnetic field, as the magnitude of ouabain's effect measured on the membrane resting potential, action potential, and interspike interval duration was higher in neurons exposed to the magnetic field. Metabolic pathways through which the magnetic field influenced the Na+/K+ pump could involve phosphorylation and dephosphorylation, as blocking these processes abolished the effect of the static magnetic field.",
publisher = "Springer",
journal = "Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology",
title = "Involvement of Na+/K+ pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field",
number = "7",
volume = "198",
doi = "10.1007/s00359-012-0727-0",
pages = "525-540"
}

Nikolić, L., Todorović, N., Zakrzewska, J. S., Stanić, M. B., Rauš Balind, S., Kalauzi, A.,& Petković, B.. (2012). Involvement of Na+/K+ pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field. in Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology
Springer., 198(7), 525-540.
https://doi.org/10.1007/s00359-012-0727-0

Nikolić L, Todorović N, Zakrzewska JS, Stanić MB, Rauš Balind S, Kalauzi A, Petković B. Involvement of Na+/K+ pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field. in Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology. 2012;198(7):525-540.
doi:10.1007/s00359-012-0727-0 .

Nikolić, Ljiljana, Todorović, Nataša, Zakrzewska, Joanna S, Stanić, Marina B, Rauš Balind, Snežana, Kalauzi, Aleksandar, Petković, Branka, "Involvement of Na+/K+ pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field" in Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology, 198, no. 7 (2012):525-540,
https://doi.org/10.1007/s00359-012-0727-0 . .

TY  - JOUR
AU  - Kalauzi, Aleksandar
AU  - Spasić, Slađana Z.
AU  - Petrović, Jelena
AU  - Ćirić, Jelena
AU  - Šaponjić, Jasna
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1175
AB  - This study was aimed to explore the sleep/wake states related cortico-pontine theta carrier frequency phase shift following a systemically induced chemical axotomy of the monoaminergic afferents within a brain of the freely moving rats. Our experiments were performed in 14 adult, male Sprague Dawley rats, chronically implanted for sleep recording. We recorded sleep during baseline condition, following sham injection (saline i.p. 1 ml/kg), and every week for 5 weeks following injection of the systemic neurotoxins (DSP-4 or PCA; 1 ml/kg, i.p.) for chemical axotomy of the locus coeruleus (LC) and dorsal raphe (DR) axon terminals. After sleep/wake states identification, FFT analysis was performed on 5 s epochs. Theta carrier frequency phase shift (Delta Phi) was calculated for each epoch by averaging theta Fourier component phase shifts, and the Delta Phi values were plotted for each rat in control condition and 28 days following the monoaminergic lesions, as a time for permanently established DR or LC chemical axotomy. Calculated group averages have shown that Delta Phi increased between pons and cortex significantly in all sleep/wake states (Wake, NREM and REM) following the monoaminergic lesions, with respect to controls. Monoaminergic lesions established the pontine leading role in the brain theta oscillations during all sleep/wake states.
T2  - General Physiology and Biophysics
T1  - Cortico-pontine theta carrier frequency phase shift across sleep/wake states following monoaminergic lesion in rat
IS  - 2
VL  - 31
EP  - 171
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1175
ER  - 

@article{
author = "Kalauzi, Aleksandar and Spasić, Slađana Z. and Petrović, Jelena and Ćirić, Jelena and Šaponjić, Jasna",
year = "2012",
abstract = "This study was aimed to explore the sleep/wake states related cortico-pontine theta carrier frequency phase shift following a systemically induced chemical axotomy of the monoaminergic afferents within a brain of the freely moving rats. Our experiments were performed in 14 adult, male Sprague Dawley rats, chronically implanted for sleep recording. We recorded sleep during baseline condition, following sham injection (saline i.p. 1 ml/kg), and every week for 5 weeks following injection of the systemic neurotoxins (DSP-4 or PCA; 1 ml/kg, i.p.) for chemical axotomy of the locus coeruleus (LC) and dorsal raphe (DR) axon terminals. After sleep/wake states identification, FFT analysis was performed on 5 s epochs. Theta carrier frequency phase shift (Delta Phi) was calculated for each epoch by averaging theta Fourier component phase shifts, and the Delta Phi values were plotted for each rat in control condition and 28 days following the monoaminergic lesions, as a time for permanently established DR or LC chemical axotomy. Calculated group averages have shown that Delta Phi increased between pons and cortex significantly in all sleep/wake states (Wake, NREM and REM) following the monoaminergic lesions, with respect to controls. Monoaminergic lesions established the pontine leading role in the brain theta oscillations during all sleep/wake states.",
journal = "General Physiology and Biophysics",
title = "Cortico-pontine theta carrier frequency phase shift across sleep/wake states following monoaminergic lesion in rat",
number = "2",
volume = "31",
pages = "171",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1175"
}

Kalauzi, A., Spasić, S. Z., Petrović, J., Ćirić, J.,& Šaponjić, J.. (2012). Cortico-pontine theta carrier frequency phase shift across sleep/wake states following monoaminergic lesion in rat. in General Physiology and Biophysics, 31(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1175

Kalauzi A, Spasić SZ, Petrović J, Ćirić J, Šaponjić J. Cortico-pontine theta carrier frequency phase shift across sleep/wake states following monoaminergic lesion in rat. in General Physiology and Biophysics. 2012;31(2):null-171.
https://hdl.handle.net/21.15107/rcub_ibiss_1175 .

Kalauzi, Aleksandar, Spasić, Slađana Z., Petrović, Jelena, Ćirić, Jelena, Šaponjić, Jasna, "Cortico-pontine theta carrier frequency phase shift across sleep/wake states following monoaminergic lesion in rat" in General Physiology and Biophysics, 31, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1175 .

TY  - JOUR
AU  - Spasić, Slađana Z.
AU  - Kalauzi, Aleksandar
AU  - Kesić, Srđan
AU  - Obradović, Milica
AU  - Šaponjić, Jasna
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1247
AB  - We used spectral analysis and Higuchi fractal dimension (FD) to correlate the EEG spectral characteristics of the sensorimotor cortex, hippocampus, and pons with their corresponding EEG signal complexities in anesthetized rats. We have explored the quantitative relationship between the mean FDs and EEG wide range high frequency (8-50 Hz) activity during ketamine/xylazine versus nembutal anesthesia at surgical plane. Using FD we detected distinct inter-structure complexity pattern and uncovered for the first time that the polygraphically and behaviorally defined anesthetized state at surgical plane as equal during experiment in two anesthetic regimens, is not the same with respect to the degree of neuronal activity (degree of generalized neuronal inhibition achieved) at different brain levels. Using the correlation of certain brain structure EEG spectral characteristics with their corresponding FDs, and the surrogate data modeling, we determined what particular frequency band contributes to EEG complexities in ketamine/xylazine versus nembutal anesthesia. In this study we have shown that the quantitative relationship between higher frequency EEG amplitude and EEG complexity is the best-modeled by surrogate data as a 3rd order polynomial. On the base of our EEG amplitude/EEG complexity relationship model, and the evidenced spectral differences in ketamine versus nembutal anesthesia we have proved that higher amplitudes of sigma, beta, and gamma frequency in ketamine anesthesia yields to higher FDs. (C) 2011 Elsevier Ltd. All rights reserved.
T2  - Journal of Theoretical Biology
T1  - Surrogate data modeling the relationship between high frequency amplitudes and Higuchi fractal dimension of EEG signals in anesthetized rats
IS  - null
VL  - 289
EP  - 166
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1247
ER  - 

@article{
author = "Spasić, Slađana Z. and Kalauzi, Aleksandar and Kesić, Srđan and Obradović, Milica and Šaponjić, Jasna",
year = "2011",
abstract = "We used spectral analysis and Higuchi fractal dimension (FD) to correlate the EEG spectral characteristics of the sensorimotor cortex, hippocampus, and pons with their corresponding EEG signal complexities in anesthetized rats. We have explored the quantitative relationship between the mean FDs and EEG wide range high frequency (8-50 Hz) activity during ketamine/xylazine versus nembutal anesthesia at surgical plane. Using FD we detected distinct inter-structure complexity pattern and uncovered for the first time that the polygraphically and behaviorally defined anesthetized state at surgical plane as equal during experiment in two anesthetic regimens, is not the same with respect to the degree of neuronal activity (degree of generalized neuronal inhibition achieved) at different brain levels. Using the correlation of certain brain structure EEG spectral characteristics with their corresponding FDs, and the surrogate data modeling, we determined what particular frequency band contributes to EEG complexities in ketamine/xylazine versus nembutal anesthesia. In this study we have shown that the quantitative relationship between higher frequency EEG amplitude and EEG complexity is the best-modeled by surrogate data as a 3rd order polynomial. On the base of our EEG amplitude/EEG complexity relationship model, and the evidenced spectral differences in ketamine versus nembutal anesthesia we have proved that higher amplitudes of sigma, beta, and gamma frequency in ketamine anesthesia yields to higher FDs. (C) 2011 Elsevier Ltd. All rights reserved.",
journal = "Journal of Theoretical Biology",
title = "Surrogate data modeling the relationship between high frequency amplitudes and Higuchi fractal dimension of EEG signals in anesthetized rats",
number = "null",
volume = "289",
pages = "166",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1247"
}

Spasić, S. Z., Kalauzi, A., Kesić, S., Obradović, M.,& Šaponjić, J.. (2011). Surrogate data modeling the relationship between high frequency amplitudes and Higuchi fractal dimension of EEG signals in anesthetized rats. in Journal of Theoretical Biology, 289(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1247

Spasić SZ, Kalauzi A, Kesić S, Obradović M, Šaponjić J. Surrogate data modeling the relationship between high frequency amplitudes and Higuchi fractal dimension of EEG signals in anesthetized rats. in Journal of Theoretical Biology. 2011;289(null):null-166.
https://hdl.handle.net/21.15107/rcub_ibiss_1247 .

Spasić, Slađana Z., Kalauzi, Aleksandar, Kesić, Srđan, Obradović, Milica, Šaponjić, Jasna, "Surrogate data modeling the relationship between high frequency amplitudes and Higuchi fractal dimension of EEG signals in anesthetized rats" in Journal of Theoretical Biology, 289, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1247 .

TY  - JOUR
AU  - Spasić, Slađana Z.
AU  - Kesić, Srđan
AU  - Kalauzi, Aleksandar
AU  - Šaponjić, Jasna
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1303
AB  - The complexity, entropy and other non-linear measures of the electroencephalogram ( EEG), such as Higuchi fractal dimension (FD), have been recently proposed as the measures of anesthesia depth and sedation. We hypothesized that during unconciousness in rats induced by the general anesthetics with opposite mechanism of action, behaviorally and poligraphically controlled as appropriately achieved stable anesthesia, we can detect distinct inter-structure brain dynamic using mean FDs. We used the surrogate data test for nonlinearity in order to establish the existence of nonlinear dynamics, and to justify the use of FD as a nonlinear measure in the time series analysis. The surrogate data of predefined probability distribution and autocorrelation properties have been generated using the algorithm of statically transformed autoregressive process (STAP). FD then is applied to quantify EEG signal complexity at the cortical, hippocampal and pontine level during stable general anesthesia (ketamine/xylazine or nembutal anesthesia). Our study showed for the first time that global neuronal inhibition caused by different mechanisms of anesthetic action induced distinct brain inter- structure complexity gradient in Sprague Dawley rats. EEG signal complexities were higher at cortical and hippocampal level in ketamine/ xylazine vs. nembutal anesthesia, with the dominance of hippocampal complexity. In nembutal anesthesia the complexity dominance moved to pontine level, and ponto- hippocampocortical decreasing complexity gradient was established. This study has proved the Higuchi fractal dimension as a valuable tool for measuring the anesthesia induced inter- structure EEG complexity.
T2  - Fractals-Complex Geometry Patterns and Scaling in Nature and Society
T1  - Different Anesthesia in Rat Induces Distinct Inter-Structure Brain Dynamic Detected By Higuchi Fractal Dimension
IS  - 1
VL  - 19
EP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1303
ER  - 

@article{
author = "Spasić, Slađana Z. and Kesić, Srđan and Kalauzi, Aleksandar and Šaponjić, Jasna",
year = "2011",
abstract = "The complexity, entropy and other non-linear measures of the electroencephalogram ( EEG), such as Higuchi fractal dimension (FD), have been recently proposed as the measures of anesthesia depth and sedation. We hypothesized that during unconciousness in rats induced by the general anesthetics with opposite mechanism of action, behaviorally and poligraphically controlled as appropriately achieved stable anesthesia, we can detect distinct inter-structure brain dynamic using mean FDs. We used the surrogate data test for nonlinearity in order to establish the existence of nonlinear dynamics, and to justify the use of FD as a nonlinear measure in the time series analysis. The surrogate data of predefined probability distribution and autocorrelation properties have been generated using the algorithm of statically transformed autoregressive process (STAP). FD then is applied to quantify EEG signal complexity at the cortical, hippocampal and pontine level during stable general anesthesia (ketamine/xylazine or nembutal anesthesia). Our study showed for the first time that global neuronal inhibition caused by different mechanisms of anesthetic action induced distinct brain inter- structure complexity gradient in Sprague Dawley rats. EEG signal complexities were higher at cortical and hippocampal level in ketamine/ xylazine vs. nembutal anesthesia, with the dominance of hippocampal complexity. In nembutal anesthesia the complexity dominance moved to pontine level, and ponto- hippocampocortical decreasing complexity gradient was established. This study has proved the Higuchi fractal dimension as a valuable tool for measuring the anesthesia induced inter- structure EEG complexity.",
journal = "Fractals-Complex Geometry Patterns and Scaling in Nature and Society",
title = "Different Anesthesia in Rat Induces Distinct Inter-Structure Brain Dynamic Detected By Higuchi Fractal Dimension",
number = "1",
volume = "19",
pages = "123",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1303"
}

Spasić, S. Z., Kesić, S., Kalauzi, A.,& Šaponjić, J.. (2011). Different Anesthesia in Rat Induces Distinct Inter-Structure Brain Dynamic Detected By Higuchi Fractal Dimension. in Fractals-Complex Geometry Patterns and Scaling in Nature and Society, 19(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1303

Spasić SZ, Kesić S, Kalauzi A, Šaponjić J. Different Anesthesia in Rat Induces Distinct Inter-Structure Brain Dynamic Detected By Higuchi Fractal Dimension. in Fractals-Complex Geometry Patterns and Scaling in Nature and Society. 2011;19(1):null-123.
https://hdl.handle.net/21.15107/rcub_ibiss_1303 .

Spasić, Slađana Z., Kesić, Srđan, Kalauzi, Aleksandar, Šaponjić, Jasna, "Different Anesthesia in Rat Induces Distinct Inter-Structure Brain Dynamic Detected By Higuchi Fractal Dimension" in Fractals-Complex Geometry Patterns and Scaling in Nature and Society, 19, no. 1 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1303 .

TY  - JOUR
AU  - Kesić, Srđan
AU  - Kalauzi, Aleksandar
AU  - Radulovacki, Miodrag
AU  - Carley, David W
AU  - Šaponjić, Jasna
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1330
AB  - Sigma and theta frequency electroencephalogram (EEG) oscillations exhibit substantial and well-recognized shifts with transitions across sleep and wake states. We aimed in this study to test the changes in coupling between these characteristic oscillations of non-rapid-eye-movement (NREM)/rapid-eye-movement (REM) sleep within and between cortical and pontine EEGs following monoaminergic lesion, by using the Pearson's product-moment correlation coefficients. Experiments were performed in 14 adult, male Sprague Dawley rats chronically instrumented for sleep recording. We lesioned the dorsal raphe nucleus axon terminals in four rats using PCA neurotoxin (p-chloroamphetamine; Sigma-Aldrich, MO) administered as two intraperitoneal (IP) injections (6 mg/kg) 24 h apart. Lesioning of locus coeruleus axon terminals was performed in five rats using DSP-4 neurotoxin (N-2-chloroethyl-N-ethyl-2-bromobenzilamine; Sigma-Aldrich, MO) in a single IP dose of 50 mg/kg. Our previous study [Saponjic et al., Physiol Behav 90:1-10, 2007] demonstrated that these systemically induced monoaminergic lesions failed to produce significant changes in sleep/wake distribution from control conditions. The present study, by using spectral analysis and by examining the Pearson's correlation coefficients and their approximate probability density (APD) distribution profiles in control and lesion condition, demonstrates significant augmentation of the sigma/theta coupling strength, an inversion of cortical sigma/theta coupling direction and emergence of an additional sigma/theta coupling "mode" specific to the post-lesion state only within the cortex. By using the Pearson's correlation coefficients and their APD profiles, instead of classical sleep/wake distribution analysis, as a measure of direction and strength of sigma/theta coupling within and between cortex and pons, we were able to uncover the impact of a tonically decreased level of brain monoamines as altered strength and mode of coupling between sigma and theta oscillations. Specifically, a new mode of sigma/theta coupling emerged following lesion, which was specific to NREM sleep, suggests that loss of monoaminergic signaling interferes with NREM sleep consolidation. Our results also indicate an importance of monoamines in control of the sleep spindle and theta rhythm generators.
T2  - Sleep and Breathing
T1  - Coupling changes in cortical and pontine sigma and theta frequency oscillations following monoaminergic lesions in rat
IS  - 1
VL  - 15
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1330
ER  - 

@article{
author = "Kesić, Srđan and Kalauzi, Aleksandar and Radulovacki, Miodrag and Carley, David W and Šaponjić, Jasna",
year = "2011",
abstract = "Sigma and theta frequency electroencephalogram (EEG) oscillations exhibit substantial and well-recognized shifts with transitions across sleep and wake states. We aimed in this study to test the changes in coupling between these characteristic oscillations of non-rapid-eye-movement (NREM)/rapid-eye-movement (REM) sleep within and between cortical and pontine EEGs following monoaminergic lesion, by using the Pearson's product-moment correlation coefficients. Experiments were performed in 14 adult, male Sprague Dawley rats chronically instrumented for sleep recording. We lesioned the dorsal raphe nucleus axon terminals in four rats using PCA neurotoxin (p-chloroamphetamine; Sigma-Aldrich, MO) administered as two intraperitoneal (IP) injections (6 mg/kg) 24 h apart. Lesioning of locus coeruleus axon terminals was performed in five rats using DSP-4 neurotoxin (N-2-chloroethyl-N-ethyl-2-bromobenzilamine; Sigma-Aldrich, MO) in a single IP dose of 50 mg/kg. Our previous study [Saponjic et al., Physiol Behav 90:1-10, 2007] demonstrated that these systemically induced monoaminergic lesions failed to produce significant changes in sleep/wake distribution from control conditions. The present study, by using spectral analysis and by examining the Pearson's correlation coefficients and their approximate probability density (APD) distribution profiles in control and lesion condition, demonstrates significant augmentation of the sigma/theta coupling strength, an inversion of cortical sigma/theta coupling direction and emergence of an additional sigma/theta coupling "mode" specific to the post-lesion state only within the cortex. By using the Pearson's correlation coefficients and their APD profiles, instead of classical sleep/wake distribution analysis, as a measure of direction and strength of sigma/theta coupling within and between cortex and pons, we were able to uncover the impact of a tonically decreased level of brain monoamines as altered strength and mode of coupling between sigma and theta oscillations. Specifically, a new mode of sigma/theta coupling emerged following lesion, which was specific to NREM sleep, suggests that loss of monoaminergic signaling interferes with NREM sleep consolidation. Our results also indicate an importance of monoamines in control of the sleep spindle and theta rhythm generators.",
journal = "Sleep and Breathing",
title = "Coupling changes in cortical and pontine sigma and theta frequency oscillations following monoaminergic lesions in rat",
number = "1",
volume = "15",
pages = "47",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1330"
}

Kesić, S., Kalauzi, A., Radulovacki, M., Carley, D. W.,& Šaponjić, J.. (2011). Coupling changes in cortical and pontine sigma and theta frequency oscillations following monoaminergic lesions in rat. in Sleep and Breathing, 15(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1330

Kesić S, Kalauzi A, Radulovacki M, Carley DW, Šaponjić J. Coupling changes in cortical and pontine sigma and theta frequency oscillations following monoaminergic lesions in rat. in Sleep and Breathing. 2011;15(1):null-47.
https://hdl.handle.net/21.15107/rcub_ibiss_1330 .

Kesić, Srđan, Kalauzi, Aleksandar, Radulovacki, Miodrag, Carley, David W, Šaponjić, Jasna, "Coupling changes in cortical and pontine sigma and theta frequency oscillations following monoaminergic lesions in rat" in Sleep and Breathing, 15, no. 1 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1330 .

TY  - JOUR
AU  - Kalauzi, Aleksandar
AU  - Kesić, Srđan
AU  - Šaponjić, Jasna
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1422
AB  - The experiments were performed in 14 adult, male Sprague Dawley rats chronically instrumented for sleep recording and recorded during baseline condition, following sham injection (saline i.p. 1 ml/kg), and every week for 5 weeks following injection of the systemic neurotoxins (DSP-4 or PCA; 1 ml/kg, i.p.) for chemical axotomy of the locus coeruleus (LC) and dorsal raphe (DR) axon terminals. In our former study we demonstrated that the systemically induced lesion of the noradrenergic or serotonergic axon terminals did not affect the sleep-wake distribution from control condition. In this Study, by using spectral analysis and phase shift spectra of the cortical and pontine EEG we analyzed cortico-pontine theta oscillation synchronization phase shift on 6-hour recordings in control condition and 28 days following the monoaminergic lesions, as a time for permanently established DR or LC chemical axotomy. Our results demonstrated for the first time that chronically decreased brain monoamines in freely moving rats changed cortico-pontine theta synchronization phase shift. Pons became a leading theta oscillator. We assume that deficit of monoamines induced predominance of the NREM/REM transitions, characterized with phasic theta oscillations (the increased density of clustered P waves which intrinsic frequency corresponds to theta frequency oscillations), and may produced preceding phasic theta versus tonic theta oscillation drive.
T2  - Journal of Physiology and Pharmacology
T1  - Cortico-Pontine Theta Synchronization Phase Shift Following Monoaminergic Lesion in Rat
IS  - 4
VL  - 60
EP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1422
ER  - 

@article{
author = "Kalauzi, Aleksandar and Kesić, Srđan and Šaponjić, Jasna",
year = "2009",
abstract = "The experiments were performed in 14 adult, male Sprague Dawley rats chronically instrumented for sleep recording and recorded during baseline condition, following sham injection (saline i.p. 1 ml/kg), and every week for 5 weeks following injection of the systemic neurotoxins (DSP-4 or PCA; 1 ml/kg, i.p.) for chemical axotomy of the locus coeruleus (LC) and dorsal raphe (DR) axon terminals. In our former study we demonstrated that the systemically induced lesion of the noradrenergic or serotonergic axon terminals did not affect the sleep-wake distribution from control condition. In this Study, by using spectral analysis and phase shift spectra of the cortical and pontine EEG we analyzed cortico-pontine theta oscillation synchronization phase shift on 6-hour recordings in control condition and 28 days following the monoaminergic lesions, as a time for permanently established DR or LC chemical axotomy. Our results demonstrated for the first time that chronically decreased brain monoamines in freely moving rats changed cortico-pontine theta synchronization phase shift. Pons became a leading theta oscillator. We assume that deficit of monoamines induced predominance of the NREM/REM transitions, characterized with phasic theta oscillations (the increased density of clustered P waves which intrinsic frequency corresponds to theta frequency oscillations), and may produced preceding phasic theta versus tonic theta oscillation drive.",
journal = "Journal of Physiology and Pharmacology",
title = "Cortico-Pontine Theta Synchronization Phase Shift Following Monoaminergic Lesion in Rat",
number = "4",
volume = "60",
pages = "84",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1422"
}

Kalauzi, A., Kesić, S.,& Šaponjić, J.. (2009). Cortico-Pontine Theta Synchronization Phase Shift Following Monoaminergic Lesion in Rat. in Journal of Physiology and Pharmacology, 60(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1422

Kalauzi A, Kesić S, Šaponjić J. Cortico-Pontine Theta Synchronization Phase Shift Following Monoaminergic Lesion in Rat. in Journal of Physiology and Pharmacology. 2009;60(4):null-84.
https://hdl.handle.net/21.15107/rcub_ibiss_1422 .

Kalauzi, Aleksandar, Kesić, Srđan, Šaponjić, Jasna, "Cortico-Pontine Theta Synchronization Phase Shift Following Monoaminergic Lesion in Rat" in Journal of Physiology and Pharmacology, 60, no. 4 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1422 .

TY  - JOUR
AU  - Bojić, Tijana
AU  - Šaponjić, Jasna
AU  - Radulovacki, Miodrag
AU  - Carley, David W
AU  - Kalauzi, Aleksandar
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1529
AB  - We applied a novel approach to respiratory waveform analysis-Monotone Signal Segments Analysis (MSSA) on 6-h recordings of respiratory signals in rats. To validate MSSA as a respiratory signal analysis tool we tested it by detecting: breaths and breath-to-breath intervals; respiratory timing and volume modes; and changes in respiratory pattern caused by lesions of monoaminergic systems in rats. MSSA differentiated three respiratory timing (tachypneic, eupneic, bradypneic-apneic), and three volume (artifacts, normovolemic, hypervolemic-sighs) modes. Lesion-induced respiratory pattern modulation was visible as shifts in the distributions of monotone signal segment amplitudes, and of breath-to-breath intervals. Specifically, noradrenergic lesion induced an increase in mean volume (p <= 0.03), with no change of the mean breath-to-breath interval duration (p >= 0.06). MSSA of timing modes detected noradrenergic lesion-induced interdependent changes in the balance of eupneic (decrease; p <= 0.02), and tachypneic (an increase; p <= 0.02) breath intervals with respect to control. In terms of breath durations within each timing mode, there was a tendency toward prolongation of the eupneic (p <= 0.08) and bradypneic-apneic (p <= 0.06) intervals. These results demonstrate that MSSA is sensitive to subtle shifts in respiratory rhythmogenesis not detectable by simple respiratory pattern descriptive statistics. MSSA represents a potentially valuable new tool for investigations of respiratory pattern control. (C) 2008 Elsevier B.V. All rights reserved.
T2  - Respiratory Physiology & Neurobiology
T1  - Monotone Signal Segments Analysis as a novel method of breath detection and breath-to-breath interval analysis in rat
IS  - 3
VL  - 161
EP  - 280
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1529
ER  - 

@article{
author = "Bojić, Tijana and Šaponjić, Jasna and Radulovacki, Miodrag and Carley, David W and Kalauzi, Aleksandar",
year = "2008",
abstract = "We applied a novel approach to respiratory waveform analysis-Monotone Signal Segments Analysis (MSSA) on 6-h recordings of respiratory signals in rats. To validate MSSA as a respiratory signal analysis tool we tested it by detecting: breaths and breath-to-breath intervals; respiratory timing and volume modes; and changes in respiratory pattern caused by lesions of monoaminergic systems in rats. MSSA differentiated three respiratory timing (tachypneic, eupneic, bradypneic-apneic), and three volume (artifacts, normovolemic, hypervolemic-sighs) modes. Lesion-induced respiratory pattern modulation was visible as shifts in the distributions of monotone signal segment amplitudes, and of breath-to-breath intervals. Specifically, noradrenergic lesion induced an increase in mean volume (p <= 0.03), with no change of the mean breath-to-breath interval duration (p >= 0.06). MSSA of timing modes detected noradrenergic lesion-induced interdependent changes in the balance of eupneic (decrease; p <= 0.02), and tachypneic (an increase; p <= 0.02) breath intervals with respect to control. In terms of breath durations within each timing mode, there was a tendency toward prolongation of the eupneic (p <= 0.08) and bradypneic-apneic (p <= 0.06) intervals. These results demonstrate that MSSA is sensitive to subtle shifts in respiratory rhythmogenesis not detectable by simple respiratory pattern descriptive statistics. MSSA represents a potentially valuable new tool for investigations of respiratory pattern control. (C) 2008 Elsevier B.V. All rights reserved.",
journal = "Respiratory Physiology & Neurobiology",
title = "Monotone Signal Segments Analysis as a novel method of breath detection and breath-to-breath interval analysis in rat",
number = "3",
volume = "161",
pages = "280",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1529"
}

Bojić, T., Šaponjić, J., Radulovacki, M., Carley, D. W.,& Kalauzi, A.. (2008). Monotone Signal Segments Analysis as a novel method of breath detection and breath-to-breath interval analysis in rat. in Respiratory Physiology & Neurobiology, 161(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1529

Bojić T, Šaponjić J, Radulovacki M, Carley DW, Kalauzi A. Monotone Signal Segments Analysis as a novel method of breath detection and breath-to-breath interval analysis in rat. in Respiratory Physiology & Neurobiology. 2008;161(3):null-280.
https://hdl.handle.net/21.15107/rcub_ibiss_1529 .

Bojić, Tijana, Šaponjić, Jasna, Radulovacki, Miodrag, Carley, David W, Kalauzi, Aleksandar, "Monotone Signal Segments Analysis as a novel method of breath detection and breath-to-breath interval analysis in rat" in Respiratory Physiology & Neurobiology, 161, no. 3 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1529 .

TY  - CONF
AU  - Spasić, Slađana Z.
AU  - Stojadinović, Gordana
AU  - Kalauzi, Aleksandar
AU  - Ćulić, Milka
AU  - Martać, Ljiljana
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1715
AB  - We recorded electrocortical activity in anesthetized rats and constructed kmax new self-similar time series, applying Higuchi's algorithm. The aim of this study was to estimate value of the parameter k(max). in order to obtain fractal dimension values as an optimum measure of biosignal change. After our analysis, electrocortical activity recordings resulted in a family of curves f(k(max)). Three regions could be distinguished 2 <= k(max). < 8, with a U-shape; 8 <= k(max) <= 30, with a steeper quasilinear increase; and k(max) >= 30, with a smaller slope quasilinear increase. We suggest the optimum region for k(max): 8 < k(max) < 18, specifically k(max) = 8.
C3  - Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus
T1  - Estimation of parameter k(max) in fractal analysis of rat brain activity
IS  - null
VL  - 1048
EP  - 429
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1715
ER  - 

@conference{
author = "Spasić, Slađana Z. and Stojadinović, Gordana and Kalauzi, Aleksandar and Ćulić, Milka and Martać, Ljiljana",
year = "2005",
abstract = "We recorded electrocortical activity in anesthetized rats and constructed kmax new self-similar time series, applying Higuchi's algorithm. The aim of this study was to estimate value of the parameter k(max). in order to obtain fractal dimension values as an optimum measure of biosignal change. After our analysis, electrocortical activity recordings resulted in a family of curves f(k(max)). Three regions could be distinguished 2 <= k(max). < 8, with a U-shape; 8 <= k(max) <= 30, with a steeper quasilinear increase; and k(max) >= 30, with a smaller slope quasilinear increase. We suggest the optimum region for k(max): 8 < k(max) < 18, specifically k(max) = 8.",
journal = "Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "Estimation of parameter k(max) in fractal analysis of rat brain activity",
number = "null",
volume = "1048",
pages = "429",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1715"
}

Spasić, S. Z., Stojadinović, G., Kalauzi, A., Ćulić, M.,& Martać, L.. (2005). Estimation of parameter k(max) in fractal analysis of rat brain activity. in Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus, 1048(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1715

Spasić SZ, Stojadinović G, Kalauzi A, Ćulić M, Martać L. Estimation of parameter k(max) in fractal analysis of rat brain activity. in Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048(null):null-429.
https://hdl.handle.net/21.15107/rcub_ibiss_1715 .

Spasić, Slađana Z., Stojadinović, Gordana, Kalauzi, Aleksandar, Ćulić, Milka, Martać, Ljiljana, "Estimation of parameter k(max) in fractal analysis of rat brain activity" in Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus, 1048, no. null (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1715 .

TY  - JOUR
AU  - Ćulić, Milka
AU  - Kalauzi, Aleksandar
AU  - Spasić, Slađana Z.
AU  - Stojadinović, Gordana
AU  - Martać, Ljiljana
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1685
AB  - We propose a new method for calculating fractal dimension (DF) of a signal y(t), based on coefficients m(y)((n)), mean absolute values of its nth order derivatives (consecutive finite differences for sampled signals). We found that logarithms of m(y)((n)), = 2, 3,..., n(max), exhibited linear dependence on n: log (m(y)((n))) = (slope)n + Y(int) with stable slopes and Y-intercepts proportional to signal DF values. Using a family of Weierstrass functions, we established a link between Y-intercepts and signal fractal dimension: DF = A(n(max))Y(int) + B(n(max)), and calculated parameters A(n(max)) and B(n(max)) for n(max) = 3,..., 7. Compared to Higuchi's algorithm, advantages of this method include greater speed and eliminating the need to choose value for k(max), since the smallest error was obtained with n(max) = 3.
T2  - Fractals-Complex Geometry Patterns and Scaling in Nature and Society
T1  - Consecutive differences as a method of signal fractal analysis
IS  - 4
VL  - 13
EP  - 292
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1685
ER  - 

@article{
author = "Ćulić, Milka and Kalauzi, Aleksandar and Spasić, Slađana Z. and Stojadinović, Gordana and Martać, Ljiljana",
year = "2005",
abstract = "We propose a new method for calculating fractal dimension (DF) of a signal y(t), based on coefficients m(y)((n)), mean absolute values of its nth order derivatives (consecutive finite differences for sampled signals). We found that logarithms of m(y)((n)), = 2, 3,..., n(max), exhibited linear dependence on n: log (m(y)((n))) = (slope)n + Y(int) with stable slopes and Y-intercepts proportional to signal DF values. Using a family of Weierstrass functions, we established a link between Y-intercepts and signal fractal dimension: DF = A(n(max))Y(int) + B(n(max)), and calculated parameters A(n(max)) and B(n(max)) for n(max) = 3,..., 7. Compared to Higuchi's algorithm, advantages of this method include greater speed and eliminating the need to choose value for k(max), since the smallest error was obtained with n(max) = 3.",
journal = "Fractals-Complex Geometry Patterns and Scaling in Nature and Society",
title = "Consecutive differences as a method of signal fractal analysis",
number = "4",
volume = "13",
pages = "292",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1685"
}

Ćulić, M., Kalauzi, A., Spasić, S. Z., Stojadinović, G.,& Martać, L.. (2005). Consecutive differences as a method of signal fractal analysis. in Fractals-Complex Geometry Patterns and Scaling in Nature and Society, 13(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1685

Ćulić M, Kalauzi A, Spasić SZ, Stojadinović G, Martać L. Consecutive differences as a method of signal fractal analysis. in Fractals-Complex Geometry Patterns and Scaling in Nature and Society. 2005;13(4):null-292.
https://hdl.handle.net/21.15107/rcub_ibiss_1685 .

Ćulić, Milka, Kalauzi, Aleksandar, Spasić, Slađana Z., Stojadinović, Gordana, Martać, Ljiljana, "Consecutive differences as a method of signal fractal analysis" in Fractals-Complex Geometry Patterns and Scaling in Nature and Society, 13, no. 4 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1685 .

TY  - JOUR
AU  - Ćulić, Milka
AU  - Kalauzi, Aleksandar
AU  - Spasić, Slađana Z.
PY  - 2003
PY  - 2003
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/95
AB  - Simple, complex action potentials (AP) and interspike recorded background activity (RBA) were detected and extracted from 12 rat cerebellar cortical recordings. For each recording, an averaged simple action potential (SAP) was formed. Fourier amplitude spectra of SAP and RBA were similar in all recordings. For one of the recordings, a series of RBA simulations (SBA) was generated, superimposing random SAPs, varying the superposition frequency (fsup). Average Fourier amplitude, Amp(SBA), was calculated, in order to use Amp(SBA)=f(fsup) as a calibration line for reading fsup of RBA, which is a measure of the population activity. A probability distribution for SAP intensities was derived and a family of calibration lines constructed. As all lines were linear and parallel (slope S) in log-log plots, a population activity change could be calculated as fsup2/fsup1=[Amp2(RBA)/Amp(RBA)]1/S.
AB  - Jednostavni, složeni akcioni potencijali (AR) i međuspajkovna osnovna aktivnost (MOA) detektovani su i izdvojeni iz 12 registrovanih signala kore malog mozga pacova. Za svaku registraciju, formiran je usrednjeni jednostavni AR (JAR). Spektri Furijeovih amplituda JAR i MOA signala bili su slični kod svih registracija. Za jednu od registracija, superponiranjem slučajnih usrednjenih jednostavnih akcionih potencijala formiran je niz simulacija međuspajkovne osnovne aktivnosti (SOA), pri čemu je varirana frekvencija superpozicije (fsup). Izračunavana je srednja Furijeova amplituda, Amp(SBA), u cilju korišćenja Amp(SBA)=f(fsup) kao kalibracione linije za očitavanje fsup od MOA, što je mera za aktivnost populacije. Izvedena je raspodela verovatnoća za intenzitete JAR, pomoću koje je konstruisana familija kalibracionih linija. Kako su sve linije linearne i paralelne u log-log dijagramu, sa nagibom S, promena aktivnosti populacije može se izračunati kao fsup2/fsup1 =[Amp2(RBA)/Amp(RBA)]1/S.
T2  - Archives of Biological Sciences
T1  - Neki detalji metode za merenje promena aktivnosti neuronskih populacija
T1  - Some details on the method for measuring activity changes of neuronal populations
IS  - 3-4
VL  - 55
SP  - 71
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_95
ER  - 

@article{
author = "Ćulić, Milka and Kalauzi, Aleksandar and Spasić, Slađana Z.",
year = "2003, 2003",
abstract = "Simple, complex action potentials (AP) and interspike recorded background activity (RBA) were detected and extracted from 12 rat cerebellar cortical recordings. For each recording, an averaged simple action potential (SAP) was formed. Fourier amplitude spectra of SAP and RBA were similar in all recordings. For one of the recordings, a series of RBA simulations (SBA) was generated, superimposing random SAPs, varying the superposition frequency (fsup). Average Fourier amplitude, Amp(SBA), was calculated, in order to use Amp(SBA)=f(fsup) as a calibration line for reading fsup of RBA, which is a measure of the population activity. A probability distribution for SAP intensities was derived and a family of calibration lines constructed. As all lines were linear and parallel (slope S) in log-log plots, a population activity change could be calculated as fsup2/fsup1=[Amp2(RBA)/Amp(RBA)]1/S., Jednostavni, složeni akcioni potencijali (AR) i međuspajkovna osnovna aktivnost (MOA) detektovani su i izdvojeni iz 12 registrovanih signala kore malog mozga pacova. Za svaku registraciju, formiran je usrednjeni jednostavni AR (JAR). Spektri Furijeovih amplituda JAR i MOA signala bili su slični kod svih registracija. Za jednu od registracija, superponiranjem slučajnih usrednjenih jednostavnih akcionih potencijala formiran je niz simulacija međuspajkovne osnovne aktivnosti (SOA), pri čemu je varirana frekvencija superpozicije (fsup). Izračunavana je srednja Furijeova amplituda, Amp(SBA), u cilju korišćenja Amp(SBA)=f(fsup) kao kalibracione linije za očitavanje fsup od MOA, što je mera za aktivnost populacije. Izvedena je raspodela verovatnoća za intenzitete JAR, pomoću koje je konstruisana familija kalibracionih linija. Kako su sve linije linearne i paralelne u log-log dijagramu, sa nagibom S, promena aktivnosti populacije može se izračunati kao fsup2/fsup1 =[Amp2(RBA)/Amp(RBA)]1/S.",
journal = "Archives of Biological Sciences",
title = "Neki detalji metode za merenje promena aktivnosti neuronskih populacija, Some details on the method for measuring activity changes of neuronal populations",
number = "3-4",
volume = "55",
pages = "71-76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_95"
}

Ćulić, M., Kalauzi, A.,& Spasić, S. Z.. (2003). Neki detalji metode za merenje promena aktivnosti neuronskih populacija. in Archives of Biological Sciences, 55(3-4), 71-76.
https://hdl.handle.net/21.15107/rcub_ibiss_95

Ćulić M, Kalauzi A, Spasić SZ. Neki detalji metode za merenje promena aktivnosti neuronskih populacija. in Archives of Biological Sciences. 2003;55(3-4):71-76.
https://hdl.handle.net/21.15107/rcub_ibiss_95 .

Ćulić, Milka, Kalauzi, Aleksandar, Spasić, Slađana Z., "Neki detalji metode za merenje promena aktivnosti neuronskih populacija" in Archives of Biological Sciences, 55, no. 3-4 (2003):71-76,
https://hdl.handle.net/21.15107/rcub_ibiss_95 .

TY  - JOUR
AU  - Ćulić, Milka
AU  - Stojadinović, Gordana
AU  - Martać, Ljiljana
AU  - Todorović, Vera N.
AU  - Drndarević, Neda Č.
AU  - Nikolić-Judith, Anna
AU  - Kalauzi, Aleksandar
AU  - Spasić, Slađana Z.
PY  - 2002
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/462
AB  - Although there has been extensive research on the effect of IGF-I on muscles and bone tissue, the effect on brain aging has received little attention. We investigated the IGF-I content in brains of differently aged rats. The IGF-I contents in cerebellar and cerebral cortex were found to be higher in immature rats (4-5 days old) compared to young adult (2.5 months old) and middle-aged (7.5-9 months old) rats. However, the decrease of mean IGF-I in middle-aged rats compared to immature animals was statistically significant only in the cerebellar codex. Our results indicate that IGF-I content decreases through the lifespan and maybe selectively in some brain regions.
AB  - Vršena su istraživanja insulinu sličnog faktora rasta (IGF-I) na mišićno i koštano tkivo, ali je posvećena mala pažnja efektu na mozak u toku starenja. Mi smo ispitivali sadržaj IGF-I u moždanom tkivu pacova različite starosti. Nađeno je da su IGF-I koncentracije u kori malog mozga kao i velikog mozga mladih pacova (4-5 dana starih) više u poređenju sa sadržajima grupe tek-odraslih pacova starosti 2,5 meseca i grupe nešto starijih odraslih pacova (7,5-9 meseci starih). Međutim, smanjenje koncentracije IGF-I sadržaja samo u kori malog mozga nešto starijih pacova (7,5-9 meseci) bilo je značajno u odnosu na vrednosti u novorođenih (4-5 dana starih pacova). Naši rezultati ukazuju da IGF-I opada tokom života i moguće - selektivno u određenim moždanim regionima.
T2  - Acta veterinaria
T1  - Starosne promene u sadržaju insulin - sličnom faktora rasta i u mozgu pacova
T1  - Age-related changes in the content of insulin: Like growth factor-l in rat brain
IS  - 5-6
VL  - 52
SP  - 299
EP  - 304
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_462
ER  - 

@article{
author = "Ćulić, Milka and Stojadinović, Gordana and Martać, Ljiljana and Todorović, Vera N. and Drndarević, Neda Č. and Nikolić-Judith, Anna and Kalauzi, Aleksandar and Spasić, Slađana Z.",
year = "2002, 2002",
abstract = "Although there has been extensive research on the effect of IGF-I on muscles and bone tissue, the effect on brain aging has received little attention. We investigated the IGF-I content in brains of differently aged rats. The IGF-I contents in cerebellar and cerebral cortex were found to be higher in immature rats (4-5 days old) compared to young adult (2.5 months old) and middle-aged (7.5-9 months old) rats. However, the decrease of mean IGF-I in middle-aged rats compared to immature animals was statistically significant only in the cerebellar codex. Our results indicate that IGF-I content decreases through the lifespan and maybe selectively in some brain regions., Vršena su istraživanja insulinu sličnog faktora rasta (IGF-I) na mišićno i koštano tkivo, ali je posvećena mala pažnja efektu na mozak u toku starenja. Mi smo ispitivali sadržaj IGF-I u moždanom tkivu pacova različite starosti. Nađeno je da su IGF-I koncentracije u kori malog mozga kao i velikog mozga mladih pacova (4-5 dana starih) više u poređenju sa sadržajima grupe tek-odraslih pacova starosti 2,5 meseca i grupe nešto starijih odraslih pacova (7,5-9 meseci starih). Međutim, smanjenje koncentracije IGF-I sadržaja samo u kori malog mozga nešto starijih pacova (7,5-9 meseci) bilo je značajno u odnosu na vrednosti u novorođenih (4-5 dana starih pacova). Naši rezultati ukazuju da IGF-I opada tokom života i moguće - selektivno u određenim moždanim regionima.",
journal = "Acta veterinaria",
title = "Starosne promene u sadržaju insulin - sličnom faktora rasta i u mozgu pacova, Age-related changes in the content of insulin: Like growth factor-l in rat brain",
number = "5-6",
volume = "52",
pages = "299-304",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_462"
}

Ćulić, M., Stojadinović, G., Martać, L., Todorović, V. N., Drndarević, N. Č., Nikolić-Judith, A., Kalauzi, A.,& Spasić, S. Z.. (2002). Starosne promene u sadržaju insulin - sličnom faktora rasta i u mozgu pacova. in Acta veterinaria, 52(5-6), 299-304.
https://hdl.handle.net/21.15107/rcub_ibiss_462

Ćulić M, Stojadinović G, Martać L, Todorović VN, Drndarević NČ, Nikolić-Judith A, Kalauzi A, Spasić SZ. Starosne promene u sadržaju insulin - sličnom faktora rasta i u mozgu pacova. in Acta veterinaria. 2002;52(5-6):299-304.
https://hdl.handle.net/21.15107/rcub_ibiss_462 .

Ćulić, Milka, Stojadinović, Gordana, Martać, Ljiljana, Todorović, Vera N., Drndarević, Neda Č., Nikolić-Judith, Anna, Kalauzi, Aleksandar, Spasić, Slađana Z., "Starosne promene u sadržaju insulin - sličnom faktora rasta i u mozgu pacova" in Acta veterinaria, 52, no. 5-6 (2002):299-304,
https://hdl.handle.net/21.15107/rcub_ibiss_462 .