TY  - JOUR
AU  - Ignjatović, Đurđica
AU  - Živković, Jelena
AU  - Tovilović-Kovačević, Gordana
AU  - Savikin, Katarina
AU  - Tomić, Mirko
AU  - Maksimović, Zoran
AU  - Jankovic, Teodora
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1973
AB  - Methanol and 70\% acetone extracts from Veronica jacquinii, Veronica
   urticifolia and Veronica teucrium were evaluated regarding their
   neuroprotective/antioxidant effects on the human neuroblastoma SH-SY5Y
   cell line. All extracts exhibited modest protective activity by
   increasing cell survival in cells stressed with sodium nitroprusside
   (9-12\%) and hydrogen peroxide (16-21\%) compared to non-treated cells.
   These activities were accompanied by reductions in the amount of
   superoxide radicals and index of lipid peroxidation. Extracts were
   further analysed for the content of total phenolics, phenylpropanoid
   glycosides and iridoids, and the major compounds acteoside and aucubin.
   The highest amount of total phenolics was observed in V. jacquinii,
   while V. teucrium was richest in total iridoids, acteoside and aucubin.
   Angiogenic properties on EA.hy926 human endothelial cells were also
   examined. At the highest non-toxic concentration (25 mu g/ml), the
   tested extracts inhibited spontaneous tube formation of endothelial
   cells on the extracellular matrix, implying their possible
   antiangiogenic activity. The most potent inhibitory effects were shown
   by methanol extract of V. jacquinii and aqueous acetone extract of V.
   teucrium. The extracts did not significantly change the adhesive or
   migratory capacity of EA.hy926 cells. Considering the traditional use of
   Veronica species, these results suggest a need for further assessment of
   their supposed wound-healing properties.
T2  - Frontiers in Life Science
T1  - Evaluation of angiogenic and neuroprotective potential of different
 extracts from three Veronica species
IS  - 2
VL  - 8
DO  - 10.1080/21553769.2014.998297
SP  - 107
EP  - 116
ER  - 

@article{
author = "Ignjatović, Đurđica and Živković, Jelena and Tovilović-Kovačević, Gordana and Savikin, Katarina and Tomić, Mirko and Maksimović, Zoran and Jankovic, Teodora",
year = "2015",
abstract = "Methanol and 70\% acetone extracts from Veronica jacquinii, Veronica
   urticifolia and Veronica teucrium were evaluated regarding their
   neuroprotective/antioxidant effects on the human neuroblastoma SH-SY5Y
   cell line. All extracts exhibited modest protective activity by
   increasing cell survival in cells stressed with sodium nitroprusside
   (9-12\%) and hydrogen peroxide (16-21\%) compared to non-treated cells.
   These activities were accompanied by reductions in the amount of
   superoxide radicals and index of lipid peroxidation. Extracts were
   further analysed for the content of total phenolics, phenylpropanoid
   glycosides and iridoids, and the major compounds acteoside and aucubin.
   The highest amount of total phenolics was observed in V. jacquinii,
   while V. teucrium was richest in total iridoids, acteoside and aucubin.
   Angiogenic properties on EA.hy926 human endothelial cells were also
   examined. At the highest non-toxic concentration (25 mu g/ml), the
   tested extracts inhibited spontaneous tube formation of endothelial
   cells on the extracellular matrix, implying their possible
   antiangiogenic activity. The most potent inhibitory effects were shown
   by methanol extract of V. jacquinii and aqueous acetone extract of V.
   teucrium. The extracts did not significantly change the adhesive or
   migratory capacity of EA.hy926 cells. Considering the traditional use of
   Veronica species, these results suggest a need for further assessment of
   their supposed wound-healing properties.",
journal = "Frontiers in Life Science",
title = "Evaluation of angiogenic and neuroprotective potential of different
 extracts from three Veronica species",
number = "2",
volume = "8",
doi = "10.1080/21553769.2014.998297",
pages = "107-116"
}

Ignjatović, Đ., Živković, J., Tovilović-Kovačević, G., Savikin, K., Tomić, M., Maksimović, Z.,& Jankovic, T.. (2015). Evaluation of angiogenic and neuroprotective potential of different
 extracts from three Veronica species. in Frontiers in Life Science, 8(2), 107-116.
https://doi.org/10.1080/21553769.2014.998297

Ignjatović Đ, Živković J, Tovilović-Kovačević G, Savikin K, Tomić M, Maksimović Z, Jankovic T. Evaluation of angiogenic and neuroprotective potential of different
 extracts from three Veronica species. in Frontiers in Life Science. 2015;8(2):107-116.
doi:10.1080/21553769.2014.998297 .

Ignjatović, Đurđica, Živković, Jelena, Tovilović-Kovačević, Gordana, Savikin, Katarina, Tomić, Mirko, Maksimović, Zoran, Jankovic, Teodora, "Evaluation of angiogenic and neuroprotective potential of different
 extracts from three Veronica species" in Frontiers in Life Science, 8, no. 2 (2015):107-116,
https://doi.org/10.1080/21553769.2014.998297 . .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Jankovic, Teodora
AU  - Pljevljakusic, Dejan
AU  - Ninković, Slavica
AU  - Smigocki, Ann
AU  - Vinterhalter, Dragan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1952
AB  - The induction and establishment of hairy root cultures of Gentiana
   dinarica using two strains of Agrobacterium rhizogenes (A4M70GUS and
   15834/PI) is reported for the first time. Strain 15834/PI had higher
   induction rate of hairy roots (32.15 \%) than strain A4M70GUS (6.12 \%).
   Transgenic nature of the roots was confirmed by GUS assay and PCR
   analysis. Two clones per strain (A4M70GUS-D and -I, and 15834/PI-2 and
   -3) with marked differences in general morphology and growth rate were
   further studied. The methanol extracts of hairy root clones were
   analyzed for xanthones content using HPLC method. The effects of the
   type of carbohydrate source (sucrose, fructose or glucose) at different
   concentrations on the growth parameters (growth index, dry weight,
   fresh/dry weight ratio), phenolic and xanthone production, and free
   radical scavenging activity of the transgenic clones were evaluated.
   Statistical two level factorial design was used to define optimal
   conditions for growth and successful secondary metabolite production in
   G. dinarica hairy root clones. The results showed that clones A4M70GUS-D
   and 15834/PI-3 were the superior ones. These two clones had the highest
   dry weight on 116.8 mM sucrose, producing up to threefold higher amounts
   of total phenolics and norswertianin-1-O-primeveroside than other
   clones, untransformed roots and roots of wild-grown plants.
T2  - Plant Cell Tissue and Organ Culture
T1  - Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production
IS  - 3
VL  - 121
DO  - 10.1007/s11240-015-0737-z
SP  - 667
EP  - 679
ER  - 

@article{
author = "Vinterhalter, Branka and Krstić Milošević, Dijana and Jankovic, Teodora and Pljevljakusic, Dejan and Ninković, Slavica and Smigocki, Ann and Vinterhalter, Dragan",
year = "2015",
abstract = "The induction and establishment of hairy root cultures of Gentiana
   dinarica using two strains of Agrobacterium rhizogenes (A4M70GUS and
   15834/PI) is reported for the first time. Strain 15834/PI had higher
   induction rate of hairy roots (32.15 \%) than strain A4M70GUS (6.12 \%).
   Transgenic nature of the roots was confirmed by GUS assay and PCR
   analysis. Two clones per strain (A4M70GUS-D and -I, and 15834/PI-2 and
   -3) with marked differences in general morphology and growth rate were
   further studied. The methanol extracts of hairy root clones were
   analyzed for xanthones content using HPLC method. The effects of the
   type of carbohydrate source (sucrose, fructose or glucose) at different
   concentrations on the growth parameters (growth index, dry weight,
   fresh/dry weight ratio), phenolic and xanthone production, and free
   radical scavenging activity of the transgenic clones were evaluated.
   Statistical two level factorial design was used to define optimal
   conditions for growth and successful secondary metabolite production in
   G. dinarica hairy root clones. The results showed that clones A4M70GUS-D
   and 15834/PI-3 were the superior ones. These two clones had the highest
   dry weight on 116.8 mM sucrose, producing up to threefold higher amounts
   of total phenolics and norswertianin-1-O-primeveroside than other
   clones, untransformed roots and roots of wild-grown plants.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production",
number = "3",
volume = "121",
doi = "10.1007/s11240-015-0737-z",
pages = "667-679"
}

Vinterhalter, B., Krstić Milošević, D., Jankovic, T., Pljevljakusic, D., Ninković, S., Smigocki, A.,& Vinterhalter, D.. (2015). Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production. in Plant Cell Tissue and Organ Culture, 121(3), 667-679.
https://doi.org/10.1007/s11240-015-0737-z

Vinterhalter B, Krstić Milošević D, Jankovic T, Pljevljakusic D, Ninković S, Smigocki A, Vinterhalter D. Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production. in Plant Cell Tissue and Organ Culture. 2015;121(3):667-679.
doi:10.1007/s11240-015-0737-z .

Vinterhalter, Branka, Krstić Milošević, Dijana, Jankovic, Teodora, Pljevljakusic, Dejan, Ninković, Slavica, Smigocki, Ann, Vinterhalter, Dragan, "Gentiana dinarica Beck. hairy root cultures and evaluation of factors
 affecting growth and xanthone production" in Plant Cell Tissue and Organ Culture, 121, no. 3 (2015):667-679,
https://doi.org/10.1007/s11240-015-0737-z . .