TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Tatalović, Nikola
AU  - Brkljačić, Jelena
AU  - Mijović, Milica
AU  - Nestorović, Vojkan
AU  - Mijušković, Ana
AU  - Oreščanin-Dušić, Zorana
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić, Milan
AU  - Spasić, Snežana
AU  - Blagojević, Duško
AU  - Miljević, Čedo
PY  - 2022
UR  - https://www.mdpi.com/1422-0067/23/22/13698
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5344
AB  - Sexual dysfunction, as a noticeable adverse effect of atypical antipsychotic drugs (APDs) for the treatment of schizophrenia, has not been investigated in detail. A study was undertaken to investigate whether 28-day long treatment with clozapine, ziprasidone or sertindole (using a recommended daily dose for atypical antipsychotic therapy), induced histopathological changes both in rat testicles and prostate, changed the activity of the antioxidant defence system and altered blood testosterone and prolactin. Clozapine, ziprasidone and sertindole induced histopathological changes in rat testicular tissue, which could be attributed to a disturbed testicular antioxidant defence system in addition to an altered prolactin to testosterone ratio. None of the APD treatments induced histopathological changes in prostate. Our results demonstrate that APDs have the capacity to change both redox and endocrinological balance. One or both outcomes could underline testicular degeneration and disturbed spermatogenesis.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis
IS  - 22
VL  - 23
DO  - 10.3390/ijms232213698
SP  - 13698
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Tatalović, Nikola and Brkljačić, Jelena and Mijović, Milica and Nestorović, Vojkan and Mijušković, Ana and Oreščanin-Dušić, Zorana and Vidonja Uzelac, Teodora and Nikolić, Milan and Spasić, Snežana and Blagojević, Duško and Miljević, Čedo",
year = "2022",
abstract = "Sexual dysfunction, as a noticeable adverse effect of atypical antipsychotic drugs (APDs) for the treatment of schizophrenia, has not been investigated in detail. A study was undertaken to investigate whether 28-day long treatment with clozapine, ziprasidone or sertindole (using a recommended daily dose for atypical antipsychotic therapy), induced histopathological changes both in rat testicles and prostate, changed the activity of the antioxidant defence system and altered blood testosterone and prolactin. Clozapine, ziprasidone and sertindole induced histopathological changes in rat testicular tissue, which could be attributed to a disturbed testicular antioxidant defence system in addition to an altered prolactin to testosterone ratio. None of the APD treatments induced histopathological changes in prostate. Our results demonstrate that APDs have the capacity to change both redox and endocrinological balance. One or both outcomes could underline testicular degeneration and disturbed spermatogenesis.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis",
number = "22",
volume = "23",
doi = "10.3390/ijms232213698",
pages = "13698"
}

Nikolić-Kokić, A., Tatalović, N., Brkljačić, J., Mijović, M., Nestorović, V., Mijušković, A., Oreščanin-Dušić, Z., Vidonja Uzelac, T., Nikolić, M., Spasić, S., Blagojević, D.,& Miljević, Č.. (2022). Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis. in International Journal of Molecular Sciences
Basel: MDPI., 23(22), 13698.
https://doi.org/10.3390/ijms232213698

Nikolić-Kokić A, Tatalović N, Brkljačić J, Mijović M, Nestorović V, Mijušković A, Oreščanin-Dušić Z, Vidonja Uzelac T, Nikolić M, Spasić S, Blagojević D, Miljević Č. Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis. in International Journal of Molecular Sciences. 2022;23(22):13698.
doi:10.3390/ijms232213698 .

Nikolić-Kokić, Aleksandra, Tatalović, Nikola, Brkljačić, Jelena, Mijović, Milica, Nestorović, Vojkan, Mijušković, Ana, Oreščanin-Dušić, Zorana, Vidonja Uzelac, Teodora, Nikolić, Milan, Spasić, Snežana, Blagojević, Duško, Miljević, Čedo, "Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis" in International Journal of Molecular Sciences, 23, no. 22 (2022):13698,
https://doi.org/10.3390/ijms232213698 . .

TY  - JOUR
AU  - Platanić Arizanović, Lena
AU  - Nikolić-Kokić, Aleksandra
AU  - Brkljačić, Jelena
AU  - Tatalović, Nikola
AU  - Miler, Marko
AU  - Oreščanin Dušić, Zorana
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić, Milan
AU  - Milošević, Verica
AU  - Blagojević, Duško
AU  - Spasić, Snežana
AU  - Miljević, Čedo
PY  - 2021
UR  - https://www.tandfonline.com/doi/abs/10.1080/15287394.2020.1844827
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4037
AB  - Chronic use of atypical antipsychotics may produce hepatic damage. Atypical antipsychotics, including clozapine, sertindole, and ziprasidone, are extensively metabolized by the liver and this process generates toxic-free radical metabolic intermediates which may contribute to liver damage. The aim of this study was to investigate whether clozapine, sertindole, or ziprasidone affected hepatic antioxidant defense enzymes which consequently led to disturbed redox homeostasis. The expression and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), and glutathione-S-transferases (GST) were measured in rat livers at doses corresponding to human antipsychotic therapy. Clozapine increased activity of SOD types 1 and 2, GR and GST, but reduced CAT activity. Sertindole elevated activities of both SODs. In ziprasidone-treated rats only decreased CAT activity was found. All three antipsychotics produced mild-to-moderate hepatic histopathological changes categorized as regenerative alterations. No apparent signs of immune cell infiltration, microvesicular or macrovesicular fatty change, or hepatocytes in mitosis were observed. In conclusion, a 4-week long daily treatment with clozapine, sertindole, or ziprasidone altered hepatic antioxidant enzyme activities and induced histopathological changes in liver. The most severe alterations were noted in clozapine-treated rats. Data indicate that redox disturbances may contribute to liver dysfunction after long-term atypical antipsychotic drug treatment.
PB  - Bellwether Publishing, Ltd.
T2  - Journal of Toxicology and Environmental Health, Part A
T1  - Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction
IS  - 4
VL  - 84
DO  - 10.1080/15287394.2020.1844827
SP  - 173
EP  - 182
ER  - 

@article{
author = "Platanić Arizanović, Lena and Nikolić-Kokić, Aleksandra and Brkljačić, Jelena and Tatalović, Nikola and Miler, Marko and Oreščanin Dušić, Zorana and Vidonja Uzelac, Teodora and Nikolić, Milan and Milošević, Verica and Blagojević, Duško and Spasić, Snežana and Miljević, Čedo",
year = "2021",
abstract = "Chronic use of atypical antipsychotics may produce hepatic damage. Atypical antipsychotics, including clozapine, sertindole, and ziprasidone, are extensively metabolized by the liver and this process generates toxic-free radical metabolic intermediates which may contribute to liver damage. The aim of this study was to investigate whether clozapine, sertindole, or ziprasidone affected hepatic antioxidant defense enzymes which consequently led to disturbed redox homeostasis. The expression and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), and glutathione-S-transferases (GST) were measured in rat livers at doses corresponding to human antipsychotic therapy. Clozapine increased activity of SOD types 1 and 2, GR and GST, but reduced CAT activity. Sertindole elevated activities of both SODs. In ziprasidone-treated rats only decreased CAT activity was found. All three antipsychotics produced mild-to-moderate hepatic histopathological changes categorized as regenerative alterations. No apparent signs of immune cell infiltration, microvesicular or macrovesicular fatty change, or hepatocytes in mitosis were observed. In conclusion, a 4-week long daily treatment with clozapine, sertindole, or ziprasidone altered hepatic antioxidant enzyme activities and induced histopathological changes in liver. The most severe alterations were noted in clozapine-treated rats. Data indicate that redox disturbances may contribute to liver dysfunction after long-term atypical antipsychotic drug treatment.",
publisher = "Bellwether Publishing, Ltd.",
journal = "Journal of Toxicology and Environmental Health, Part A",
title = "Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction",
number = "4",
volume = "84",
doi = "10.1080/15287394.2020.1844827",
pages = "173-182"
}

Platanić Arizanović, L., Nikolić-Kokić, A., Brkljačić, J., Tatalović, N., Miler, M., Oreščanin Dušić, Z., Vidonja Uzelac, T., Nikolić, M., Milošević, V., Blagojević, D., Spasić, S.,& Miljević, Č.. (2021). Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction. in Journal of Toxicology and Environmental Health, Part A
Bellwether Publishing, Ltd.., 84(4), 173-182.
https://doi.org/10.1080/15287394.2020.1844827

Platanić Arizanović L, Nikolić-Kokić A, Brkljačić J, Tatalović N, Miler M, Oreščanin Dušić Z, Vidonja Uzelac T, Nikolić M, Milošević V, Blagojević D, Spasić S, Miljević Č. Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction. in Journal of Toxicology and Environmental Health, Part A. 2021;84(4):173-182.
doi:10.1080/15287394.2020.1844827 .

Platanić Arizanović, Lena, Nikolić-Kokić, Aleksandra, Brkljačić, Jelena, Tatalović, Nikola, Miler, Marko, Oreščanin Dušić, Zorana, Vidonja Uzelac, Teodora, Nikolić, Milan, Milošević, Verica, Blagojević, Duško, Spasić, Snežana, Miljević, Čedo, "Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction" in Journal of Toxicology and Environmental Health, Part A, 84, no. 4 (2021):173-182,
https://doi.org/10.1080/15287394.2020.1844827 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Mijušković, Ana
AU  - Tatalović, Nikola
AU  - Brkljačić, Jelena
AU  - Miler, Marko
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić, Milan
AU  - Milošević, Verica
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
AU  - Miljević, Čedo
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/123456789/3900
AB  - The use of atypical antipsychotic drugs (APD) was reported to be associated with adverse effects
on the kidneys. Thus, the aim of this study was to examine whether APD exerted their adverse
effects by interfering with the renal antioxidant defense system. Male 3-mo-old Wistar rats were
treated for 28 d with ziprasidone (ZIP), clozapine (CLO), or sertindole (SER) using a daily dose
recommended for antipsychotic drug therapy. The expression and activities of antioxidant
enzymes superoxide dismutase (SOD) type 1 and type 2, catalase (CAT), glutathione reductase
(GR), and glutathione S-transferases (GSTs) activity were measured in the kidneys. Changes in the
kidneys were also evaluated histologically. Ziprasidone, CLO, and SER reduced renal SOD type 1
and type 2 activities. Decreased CAT activity was observed only in SER-treated rats. An inhibition
in GR activity and increased activity of GST was found only after treatment with CLO. Histological
analysis showed dilatation of proximal tubules in kidneys with all three drugs. In conclusion, data
indicate that redox disturbances may contribute to renal morphologic alterations in proximal
tubules in rats treated with all APD.
PB  - Taylor & Francis
T2  - Journal of Toxicology and Environmental Health, Part A: Current Issues
T1  - Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney
IS  - 20
VL  - 79
DO  - 10.1080/15287394.2016.1201706
SP  - 905
EP  - 911
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Mijušković, Ana and Tatalović, Nikola and Brkljačić, Jelena and Miler, Marko and Oreščanin Dušić, Zorana and Nikolić, Milan and Milošević, Verica and Blagojević, Duško and Spasić, Mihajlo and Miljević, Čedo",
year = "2016",
abstract = "The use of atypical antipsychotic drugs (APD) was reported to be associated with adverse effects
on the kidneys. Thus, the aim of this study was to examine whether APD exerted their adverse
effects by interfering with the renal antioxidant defense system. Male 3-mo-old Wistar rats were
treated for 28 d with ziprasidone (ZIP), clozapine (CLO), or sertindole (SER) using a daily dose
recommended for antipsychotic drug therapy. The expression and activities of antioxidant
enzymes superoxide dismutase (SOD) type 1 and type 2, catalase (CAT), glutathione reductase
(GR), and glutathione S-transferases (GSTs) activity were measured in the kidneys. Changes in the
kidneys were also evaluated histologically. Ziprasidone, CLO, and SER reduced renal SOD type 1
and type 2 activities. Decreased CAT activity was observed only in SER-treated rats. An inhibition
in GR activity and increased activity of GST was found only after treatment with CLO. Histological
analysis showed dilatation of proximal tubules in kidneys with all three drugs. In conclusion, data
indicate that redox disturbances may contribute to renal morphologic alterations in proximal
tubules in rats treated with all APD.",
publisher = "Taylor & Francis",
journal = "Journal of Toxicology and Environmental Health, Part A: Current Issues",
title = "Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney",
number = "20",
volume = "79",
doi = "10.1080/15287394.2016.1201706",
pages = "905-911"
}

Nikolić-Kokić, A., Mijušković, A., Tatalović, N., Brkljačić, J., Miler, M., Oreščanin Dušić, Z., Nikolić, M., Milošević, V., Blagojević, D., Spasić, M.,& Miljević, Č.. (2016). Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney. in Journal of Toxicology and Environmental Health, Part A: Current Issues
Taylor & Francis., 79(20), 905-911.
https://doi.org/10.1080/15287394.2016.1201706

Nikolić-Kokić A, Mijušković A, Tatalović N, Brkljačić J, Miler M, Oreščanin Dušić Z, Nikolić M, Milošević V, Blagojević D, Spasić M, Miljević Č. Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney. in Journal of Toxicology and Environmental Health, Part A: Current Issues. 2016;79(20):905-911.
doi:10.1080/15287394.2016.1201706 .

Nikolić-Kokić, Aleksandra, Mijušković, Ana, Tatalović, Nikola, Brkljačić, Jelena, Miler, Marko, Oreščanin Dušić, Zorana, Nikolić, Milan, Milošević, Verica, Blagojević, Duško, Spasić, Mihajlo, Miljević, Čedo, "Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney" in Journal of Toxicology and Environmental Health, Part A: Current Issues, 79, no. 20 (2016):905-911,
https://doi.org/10.1080/15287394.2016.1201706 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Mijušković, Ana
AU  - Tatalović, Nikola
AU  - Brkljačić, Jelena
AU  - Miler, Marko
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić, Milan
AU  - Milošević, Verica
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
AU  - Miljević, Čedo
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4042
AB  - The use of atypical antipsychotic drugs (APD) was reported to be associated with adverse effectson the kidneys. Thus, the aim of this study was to examine whether APD exerted their adverseeffects by interfering with the renal antioxidant defense system. Male 3-mo-old Wistar rats weretreated for 28 d with ziprasidone (ZIP), clozapine (CLO), or sertindole (SER) using a daily doserecommended for antipsychotic drug therapy. The expression and activities of antioxidantenzymes superoxide dismutase (SOD) type 1 and type 2, catalase (CAT), glutathione reductase(GR), and glutathione S-transferases (GSTs) activity were measured in the kidneys. Changes in thekidneys were also evaluated histologically. Ziprasidone, CLO, and SER reduced renal SOD type 1and type 2 activities. Decreased CAT activity was observed only in SER-treated rats. An inhibitionin GR activity and increased activity of GST was found only after treatment with CLO. Histologicalanalysis showed dilatation of proximal tubules in kidneys with all three drugs. In conclusion, dataindicate that redox disturbances may contribute to renal morphologic alterations in proximaltubules in rats treated with all APD.
PB  - Taylor & Francis
T2  - Journal of Toxicology and Environmental Health, Part A: Current Issues
T1  - Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney
IS  - 20
VL  - 79
DO  - 10.1080/15287394.2016.1201706
SP  - 905
EP  - 911
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Mijušković, Ana and Tatalović, Nikola and Brkljačić, Jelena and Miler, Marko and Oreščanin Dušić, Zorana and Nikolić, Milan and Milošević, Verica and Blagojević, Duško and Spasić, Mihajlo and Miljević, Čedo",
year = "2016",
abstract = "The use of atypical antipsychotic drugs (APD) was reported to be associated with adverse effectson the kidneys. Thus, the aim of this study was to examine whether APD exerted their adverseeffects by interfering with the renal antioxidant defense system. Male 3-mo-old Wistar rats weretreated for 28 d with ziprasidone (ZIP), clozapine (CLO), or sertindole (SER) using a daily doserecommended for antipsychotic drug therapy. The expression and activities of antioxidantenzymes superoxide dismutase (SOD) type 1 and type 2, catalase (CAT), glutathione reductase(GR), and glutathione S-transferases (GSTs) activity were measured in the kidneys. Changes in thekidneys were also evaluated histologically. Ziprasidone, CLO, and SER reduced renal SOD type 1and type 2 activities. Decreased CAT activity was observed only in SER-treated rats. An inhibitionin GR activity and increased activity of GST was found only after treatment with CLO. Histologicalanalysis showed dilatation of proximal tubules in kidneys with all three drugs. In conclusion, dataindicate that redox disturbances may contribute to renal morphologic alterations in proximaltubules in rats treated with all APD.",
publisher = "Taylor & Francis",
journal = "Journal of Toxicology and Environmental Health, Part A: Current Issues",
title = "Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney",
number = "20",
volume = "79",
doi = "10.1080/15287394.2016.1201706",
pages = "905-911"
}

Nikolić-Kokić, A., Mijušković, A., Tatalović, N., Brkljačić, J., Miler, M., Oreščanin Dušić, Z., Nikolić, M., Milošević, V., Blagojević, D., Spasić, M.,& Miljević, Č.. (2016). Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney. in Journal of Toxicology and Environmental Health, Part A: Current Issues
Taylor & Francis., 79(20), 905-911.
https://doi.org/10.1080/15287394.2016.1201706

Nikolić-Kokić A, Mijušković A, Tatalović N, Brkljačić J, Miler M, Oreščanin Dušić Z, Nikolić M, Milošević V, Blagojević D, Spasić M, Miljević Č. Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney. in Journal of Toxicology and Environmental Health, Part A: Current Issues. 2016;79(20):905-911.
doi:10.1080/15287394.2016.1201706 .

Nikolić-Kokić, Aleksandra, Mijušković, Ana, Tatalović, Nikola, Brkljačić, Jelena, Miler, Marko, Oreščanin Dušić, Zorana, Nikolić, Milan, Milošević, Verica, Blagojević, Duško, Spasić, Mihajlo, Miljević, Čedo, "Effects of antipsychotic drug administration on antioxidative defense enzymes in male rat kidney" in Journal of Toxicology and Environmental Health, Part A: Current Issues, 79, no. 20 (2016):905-911,
https://doi.org/10.1080/15287394.2016.1201706 . .

TY  - JOUR
AU  - Nikolić, Milan
AU  - Blagojević, Duško
AU  - Nikolić-Kokić, Aleksandra
AU  - Stanić, Dragana
AU  - Vranić, Danijela
AU  - Jones, David R.
AU  - Niketić, Vesna
AU  - Spasić, Mihajlo
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/552
AB  - In a previous study, it was shown that the lipid fraction, which is occasionally observed in red blood cell hemolysates, represents cholesterol (Ch) associated with phospholipid firmly bound to haemoglobin (termed Hb-Ch). The current study was conducted to investigate whether Hb-Ch could affect the primary anti-oxidant enzyme defence system in human erythrocytes. Sixty healthy volunteers were used for the current study. Group 1 consisted of 28 subjects without or with a low level of Hb-Ch. Group 2 comprised 32 subjects with a considerably higher level of Hb-Ch. The activities of erythrocyte superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, as well as the content of methaemoglobin (metHb) were measured in both groups. The results indicated that the amount of Hb-Ch neither influenced the activities of the erythrocyte anti-oxidant enzymes nor altered the level of metHb. However, a higher amount of Hb-Ch changed the correlations in the part of the anti-oxidant defence system relating to glutathione, suggesting increased peroxidative pressure from plasma lipids. Group 2 also had significantly increased concentrations of total plasma Ch and triglycerides. Together, these facts are strong indications that the anti-oxidant defence system in human erythrocytes finely retunes its composition according to plasma oxidative demands. .
AB  - U prethodnom radu pokazano je da lipidna frakcija koja se javlja u hemolizatu zdravih ljudi predstavlja holesterol (asosovan sa fosfolipidima) čvrsto vezan za hemoglobin (Hb-Ch). U ovom radu ispitivan je uticaj Hb-Ch na anti-oksidativni enzimski sistem u humanim eritrocitima. Određena je aktivnost superoksid-dizmutaze, katalaze, glutation-peroksidaze i glutation-reduktaze, kao i sadržaj met-hemoglobina (metHb) u eritrocitima 60 ljudi, podeljenih u dve grupe na osnovu količine Hb-Ch. Rezultati pokazuju da količina prisutnog Hb-Ch ne menja aktivnost merenih enzima, niti nivo metHb. Međutim, u grupi ispitanika sa povećanim sadržajem Hb-Ch zapažene su korelativne promene u delu anti-oksidativnog enzimskog sistema povezanog sa glutationom. U istoj grupi detektovane su i veće koncentracije ukupnog holesterola i triglicerida u plazmi, što zajedno ukazuje na povećani peroksidativni pritisak iz plazme. Ovi rezultati ukazuju da odbrambeni anti-oksidativni enzimski sistem u humanim eritrocitima prilagođava svoju organizaciju prema zahtevima iz svog okruženja. .
T2  - Journal of the Serbian Chemical Society
T1  - Da li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima?
T1  - Does cholesterol bound to haemoglobin affect the anti-oxidant enzyme defence system in human erythrocytes?
IS  - 4
VL  - 72
SP  - 339
EP  - 345
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_552
ER  - 

@article{
author = "Nikolić, Milan and Blagojević, Duško and Nikolić-Kokić, Aleksandra and Stanić, Dragana and Vranić, Danijela and Jones, David R. and Niketić, Vesna and Spasić, Mihajlo",
year = "2007, 2007",
abstract = "In a previous study, it was shown that the lipid fraction, which is occasionally observed in red blood cell hemolysates, represents cholesterol (Ch) associated with phospholipid firmly bound to haemoglobin (termed Hb-Ch). The current study was conducted to investigate whether Hb-Ch could affect the primary anti-oxidant enzyme defence system in human erythrocytes. Sixty healthy volunteers were used for the current study. Group 1 consisted of 28 subjects without or with a low level of Hb-Ch. Group 2 comprised 32 subjects with a considerably higher level of Hb-Ch. The activities of erythrocyte superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, as well as the content of methaemoglobin (metHb) were measured in both groups. The results indicated that the amount of Hb-Ch neither influenced the activities of the erythrocyte anti-oxidant enzymes nor altered the level of metHb. However, a higher amount of Hb-Ch changed the correlations in the part of the anti-oxidant defence system relating to glutathione, suggesting increased peroxidative pressure from plasma lipids. Group 2 also had significantly increased concentrations of total plasma Ch and triglycerides. Together, these facts are strong indications that the anti-oxidant defence system in human erythrocytes finely retunes its composition according to plasma oxidative demands. ., U prethodnom radu pokazano je da lipidna frakcija koja se javlja u hemolizatu zdravih ljudi predstavlja holesterol (asosovan sa fosfolipidima) čvrsto vezan za hemoglobin (Hb-Ch). U ovom radu ispitivan je uticaj Hb-Ch na anti-oksidativni enzimski sistem u humanim eritrocitima. Određena je aktivnost superoksid-dizmutaze, katalaze, glutation-peroksidaze i glutation-reduktaze, kao i sadržaj met-hemoglobina (metHb) u eritrocitima 60 ljudi, podeljenih u dve grupe na osnovu količine Hb-Ch. Rezultati pokazuju da količina prisutnog Hb-Ch ne menja aktivnost merenih enzima, niti nivo metHb. Međutim, u grupi ispitanika sa povećanim sadržajem Hb-Ch zapažene su korelativne promene u delu anti-oksidativnog enzimskog sistema povezanog sa glutationom. U istoj grupi detektovane su i veće koncentracije ukupnog holesterola i triglicerida u plazmi, što zajedno ukazuje na povećani peroksidativni pritisak iz plazme. Ovi rezultati ukazuju da odbrambeni anti-oksidativni enzimski sistem u humanim eritrocitima prilagođava svoju organizaciju prema zahtevima iz svog okruženja. .",
journal = "Journal of the Serbian Chemical Society",
title = "Da li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima?, Does cholesterol bound to haemoglobin affect the anti-oxidant enzyme defence system in human erythrocytes?",
number = "4",
volume = "72",
pages = "339-345",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_552"
}

Nikolić, M., Blagojević, D., Nikolić-Kokić, A., Stanić, D., Vranić, D., Jones, D. R., Niketić, V.,& Spasić, M.. (2007). Da li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima?. in Journal of the Serbian Chemical Society, 72(4), 339-345.
https://hdl.handle.net/21.15107/rcub_ibiss_552

Nikolić M, Blagojević D, Nikolić-Kokić A, Stanić D, Vranić D, Jones DR, Niketić V, Spasić M. Da li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima?. in Journal of the Serbian Chemical Society. 2007;72(4):339-345.
https://hdl.handle.net/21.15107/rcub_ibiss_552 .

Nikolić, Milan, Blagojević, Duško, Nikolić-Kokić, Aleksandra, Stanić, Dragana, Vranić, Danijela, Jones, David R., Niketić, Vesna, Spasić, Mihajlo, "Da li holesterol vezan za hemoglobin utiče na anti-oksidativni enzimski sistem u humanim eritrocitima?" in Journal of the Serbian Chemical Society, 72, no. 4 (2007):339-345,
https://hdl.handle.net/21.15107/rcub_ibiss_552 .

TY  - JOUR
AU  - Stojanović, Srđan D.
AU  - Spasić, Mihajlo
AU  - Stanić, Dragana
AU  - Nikolić, Milan
AU  - Raičević, Smiljana
AU  - Niketić, Vesna
PY  - 2005
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/550
AB  - The peroxynitrite-induced nitration of manganese superoxide dismutase (MnSOD) tyrosine residue, which causes enzyme inactivation, is well established. This led to suggestions that MnSOD nitration and inactivation in vivo, detected in various diseases associated with oxidative stress and overproduction of nitric monoxide (NO), conditions which favor peroxynitrite formation, is also caused by peroxynitrite. However, our previous in vitro study demonstrated that exposure of MnSOD to NO led to NO conversion into nitrosonium (NO+) and nitroxyl (NO–) species, which caused enzyme modifications and inactivation. Here it is reported that MnSOD is tyrosine nitrated upon exposure to NO, as well as that MnSOD nitration contributes to inactivation of the enzyme. Collectively, these observations provide a compelling argument supporting the generation of nitrating species in MnSOD exposed to NO and shed a new light on MnSOD tyrosine nitration and inactivation in vivo. This may represent a novel mechanism by which MnSOD protects cell from deleterious effects associated with overproduction of NO. However, extensive MnSOD modification and inactivation associated with prolonged exposure to NO will amplify the toxic effects caused by increased cell superoxide and NO levels.
AB  - Dobro je poznato da peroksinitrit izaziva nitrovanje ostataka tirozina u mangan-superoksid- dismutazi (MnSOD) što dovodi do inaktivacije enzima. Pokazano je da nitrovanje i inaktivacija MnSOD-a nastaje u raznim bolestima za koje je karakteristič an oksidativni stres i povećana produkcija azot-monoksida (NO). Pošto se pri ovim uslovima očekuje nastajanje peroksinitrita predloženo je da peroksinitrit izaziva nitrovanje i inaktivaciju MnSOD in vivo. U našem prethodnom radu pokazali smo da MnSOD katalizuje transformaciju NO u nitrozonijum (NO+) i nitroksil (NO–) reaktivne vrste, te identifikovali neke od modifikacija molekula enzima koje pri tome nastaju izazivajući njegovu inaktivaciju. U ovom radu je pokazano da pri izlaganju MnSOD azot-monoksidu dolazi i do nitrovanja ostatka tirozina u molekulu enzima, što doprinosi njegovoj inaktivaciji. Ovi rezultati ukazuju da pri interakciji MnSOD sa NO dolazi do nastajanja nitrujućih vrsta, što baca novo svetlo na proces nitrovanja ostataka tirozina i inaktivaciju MnSOD in vivo. Ovo može da predstavlja novi mehanizam kojim MnSOD štiti ćeliju odštetnih efekata izazvanih hiperprodukcijom azot-monoksida. Međutim ekstenzivne modifikacije i inaktivacija MnSOD do kojih dolazi pri produženom izlaganju enzima NO, uvećaće toksične efekte izazvane povećanim koncentracijama superoksida i NO u ćeliji.
T2  - Journal of the Serbian Chemical Society
T1  - Mangan-superoksid-dismutaza (MnSOD) katalizuje NO-zavisno nitrovanje ostatka tirozina
T1  - Manganese superoxide dismutase (MnSOD) catalyzes NO-dependent tyrosine residue nitration
IS  - 4
VL  - 70
SP  - 601
EP  - 608
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_550
ER  - 

@article{
author = "Stojanović, Srđan D. and Spasić, Mihajlo and Stanić, Dragana and Nikolić, Milan and Raičević, Smiljana and Niketić, Vesna",
year = "2005, 2005",
abstract = "The peroxynitrite-induced nitration of manganese superoxide dismutase (MnSOD) tyrosine residue, which causes enzyme inactivation, is well established. This led to suggestions that MnSOD nitration and inactivation in vivo, detected in various diseases associated with oxidative stress and overproduction of nitric monoxide (NO), conditions which favor peroxynitrite formation, is also caused by peroxynitrite. However, our previous in vitro study demonstrated that exposure of MnSOD to NO led to NO conversion into nitrosonium (NO+) and nitroxyl (NO–) species, which caused enzyme modifications and inactivation. Here it is reported that MnSOD is tyrosine nitrated upon exposure to NO, as well as that MnSOD nitration contributes to inactivation of the enzyme. Collectively, these observations provide a compelling argument supporting the generation of nitrating species in MnSOD exposed to NO and shed a new light on MnSOD tyrosine nitration and inactivation in vivo. This may represent a novel mechanism by which MnSOD protects cell from deleterious effects associated with overproduction of NO. However, extensive MnSOD modification and inactivation associated with prolonged exposure to NO will amplify the toxic effects caused by increased cell superoxide and NO levels., Dobro je poznato da peroksinitrit izaziva nitrovanje ostataka tirozina u mangan-superoksid- dismutazi (MnSOD) što dovodi do inaktivacije enzima. Pokazano je da nitrovanje i inaktivacija MnSOD-a nastaje u raznim bolestima za koje je karakteristič an oksidativni stres i povećana produkcija azot-monoksida (NO). Pošto se pri ovim uslovima očekuje nastajanje peroksinitrita predloženo je da peroksinitrit izaziva nitrovanje i inaktivaciju MnSOD in vivo. U našem prethodnom radu pokazali smo da MnSOD katalizuje transformaciju NO u nitrozonijum (NO+) i nitroksil (NO–) reaktivne vrste, te identifikovali neke od modifikacija molekula enzima koje pri tome nastaju izazivajući njegovu inaktivaciju. U ovom radu je pokazano da pri izlaganju MnSOD azot-monoksidu dolazi i do nitrovanja ostatka tirozina u molekulu enzima, što doprinosi njegovoj inaktivaciji. Ovi rezultati ukazuju da pri interakciji MnSOD sa NO dolazi do nastajanja nitrujućih vrsta, što baca novo svetlo na proces nitrovanja ostataka tirozina i inaktivaciju MnSOD in vivo. Ovo može da predstavlja novi mehanizam kojim MnSOD štiti ćeliju odštetnih efekata izazvanih hiperprodukcijom azot-monoksida. Međutim ekstenzivne modifikacije i inaktivacija MnSOD do kojih dolazi pri produženom izlaganju enzima NO, uvećaće toksične efekte izazvane povećanim koncentracijama superoksida i NO u ćeliji.",
journal = "Journal of the Serbian Chemical Society",
title = "Mangan-superoksid-dismutaza (MnSOD) katalizuje NO-zavisno nitrovanje ostatka tirozina, Manganese superoxide dismutase (MnSOD) catalyzes NO-dependent tyrosine residue nitration",
number = "4",
volume = "70",
pages = "601-608",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_550"
}

Stojanović, S. D., Spasić, M., Stanić, D., Nikolić, M., Raičević, S.,& Niketić, V.. (2005). Mangan-superoksid-dismutaza (MnSOD) katalizuje NO-zavisno nitrovanje ostatka tirozina. in Journal of the Serbian Chemical Society, 70(4), 601-608.
https://hdl.handle.net/21.15107/rcub_ibiss_550

Stojanović SD, Spasić M, Stanić D, Nikolić M, Raičević S, Niketić V. Mangan-superoksid-dismutaza (MnSOD) katalizuje NO-zavisno nitrovanje ostatka tirozina. in Journal of the Serbian Chemical Society. 2005;70(4):601-608.
https://hdl.handle.net/21.15107/rcub_ibiss_550 .

Stojanović, Srđan D., Spasić, Mihajlo, Stanić, Dragana, Nikolić, Milan, Raičević, Smiljana, Niketić, Vesna, "Mangan-superoksid-dismutaza (MnSOD) katalizuje NO-zavisno nitrovanje ostatka tirozina" in Journal of the Serbian Chemical Society, 70, no. 4 (2005):601-608,
https://hdl.handle.net/21.15107/rcub_ibiss_550 .