TY  - JOUR
AU  - Jovičić, Katarina
AU  - Đikanović, Vesna
AU  - Santrač, Isidora
AU  - Živković, Sanja
AU  - Dimitrijević, Milena
AU  - Vranković, Jelena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6636
AB  - In this study, the concentrations of metals and trace elements (As, Cd, Co, Cr, Cu, Hg, Ni,
Pb and Zn) were determined in the muscle tissue of adult roach and white bream at two different
sites in the Belgrade section of the Danube. Twenty-six fatty acids, consisting of nine saturated FA
(SFAs), seven monosaturated FA (MUFAs) and ten polysaturated FA (PUFAs), were identified. The
analysis of the concentration of metals and trace elements of the roach and white bream showed
species-specific differences in their bioaccumulation. Four of all elements analyzed (As, Hg, Ni and
Pb) correlated significantly with the changes in FA profiles in fish from both sampling sites, with the
exception of Cu, which correlated with the FA profile at the site before, and Zn, whose concentration
influenced the FA profile at the site after wastewater discharges. The lower PUFA content in the fish
from a site under higher environment pressure could indicate that the fish are stressed. The results
suggest that changes in lipid composition may be one of the protective mechanisms of cells to cope
with anthropogenic stressors.
PB  - Basel: MDPI
T2  - Animals
T1  - Effects of Trace Elements on the Fatty Acid Composition in Danubian Fish Species
IS  - 954
VL  - 14
DO  - 10.3390/ani14060954
SP  - 954
ER  - 

@article{
author = "Jovičić, Katarina and Đikanović, Vesna and Santrač, Isidora and Živković, Sanja and Dimitrijević, Milena and Vranković, Jelena",
year = "2024",
abstract = "In this study, the concentrations of metals and trace elements (As, Cd, Co, Cr, Cu, Hg, Ni,
Pb and Zn) were determined in the muscle tissue of adult roach and white bream at two different
sites in the Belgrade section of the Danube. Twenty-six fatty acids, consisting of nine saturated FA
(SFAs), seven monosaturated FA (MUFAs) and ten polysaturated FA (PUFAs), were identified. The
analysis of the concentration of metals and trace elements of the roach and white bream showed
species-specific differences in their bioaccumulation. Four of all elements analyzed (As, Hg, Ni and
Pb) correlated significantly with the changes in FA profiles in fish from both sampling sites, with the
exception of Cu, which correlated with the FA profile at the site before, and Zn, whose concentration
influenced the FA profile at the site after wastewater discharges. The lower PUFA content in the fish
from a site under higher environment pressure could indicate that the fish are stressed. The results
suggest that changes in lipid composition may be one of the protective mechanisms of cells to cope
with anthropogenic stressors.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "Effects of Trace Elements on the Fatty Acid Composition in Danubian Fish Species",
number = "954",
volume = "14",
doi = "10.3390/ani14060954",
pages = "954"
}

Jovičić, K., Đikanović, V., Santrač, I., Živković, S., Dimitrijević, M.,& Vranković, J.. (2024). Effects of Trace Elements on the Fatty Acid Composition in Danubian Fish Species. in Animals
Basel: MDPI., 14(954), 954.
https://doi.org/10.3390/ani14060954

Jovičić K, Đikanović V, Santrač I, Živković S, Dimitrijević M, Vranković J. Effects of Trace Elements on the Fatty Acid Composition in Danubian Fish Species. in Animals. 2024;14(954):954.
doi:10.3390/ani14060954 .

Jovičić, Katarina, Đikanović, Vesna, Santrač, Isidora, Živković, Sanja, Dimitrijević, Milena, Vranković, Jelena, "Effects of Trace Elements on the Fatty Acid Composition in Danubian Fish Species" in Animals, 14, no. 954 (2024):954,
https://doi.org/10.3390/ani14060954 . .

TY  - GEN
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Dmitrović, Slavica
AU  - Petrović, Luka
AU  - Filipović, Biljana
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Anđelković, Boban
AU  - Dimitrijević, Milena
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6196
AB  - Nepeta L. holds a prominent position as the largest genus in the Lamiaceae family and serves as the exclusive representative of the iridoid-lacking Nepetoidae subfamily which produces iridoids. The genus showcases a rich chemodiversity, encompassing taxa both with and without iridoids. Our study investigates the genetic basis of iridoid diversity using omics-guided and functional genomics approaches. We identified functional iridoid synthases (ISY) in iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa L. (NnIS). Remarkably, N. nervosa carries a dormant iridoid biosynthetic platform, suggesting a loss of iridoid production during its evolutionary history. Moreover, we explore regulatory mechanisms through comparative iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs) under various stress conditions (e.g., dehydration, UV − B radiation, pathogens) or elicitors (MeJA). These mechanisms influence plant productivity and the presence/absence of iridoids or their specific groups (iridoid aglycones and iridoid glycosides). Our research offers valuable insights into the molecular mechanisms driving iridoid biosynthesis and the chemical evolution of iridoids within Nepeta. This study enhances our understanding of the intricate relationship between genetics and the environment, providing comprehensive insights into iridoid metabolism, chemical evolution, and ecology.
PB  - Belgrade: Faculty of Chemistry
T2  - Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
T1  - Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme
EP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6196
ER  - 

@misc{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Božunović, Jelena and Nestorović Živković, Jasmina and Gašić, Uroš and Milutinović, Milica and Dmitrović, Slavica and Petrović, Luka and Filipović, Biljana and Banjanac, Tijana and Šiler, Branislav and Anđelković, Boban and Dimitrijević, Milena and Mišić, Danijela",
year = "2023",
abstract = "Nepeta L. holds a prominent position as the largest genus in the Lamiaceae family and serves as the exclusive representative of the iridoid-lacking Nepetoidae subfamily which produces iridoids. The genus showcases a rich chemodiversity, encompassing taxa both with and without iridoids. Our study investigates the genetic basis of iridoid diversity using omics-guided and functional genomics approaches. We identified functional iridoid synthases (ISY) in iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa L. (NnIS). Remarkably, N. nervosa carries a dormant iridoid biosynthetic platform, suggesting a loss of iridoid production during its evolutionary history. Moreover, we explore regulatory mechanisms through comparative iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs) under various stress conditions (e.g., dehydration, UV − B radiation, pathogens) or elicitors (MeJA). These mechanisms influence plant productivity and the presence/absence of iridoids or their specific groups (iridoid aglycones and iridoid glycosides). Our research offers valuable insights into the molecular mechanisms driving iridoid biosynthesis and the chemical evolution of iridoids within Nepeta. This study enhances our understanding of the intricate relationship between genetics and the environment, providing comprehensive insights into iridoid metabolism, chemical evolution, and ecology.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia",
title = "Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme",
pages = "156",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6196"
}

Aničić, N., Matekalo, D., Skorić, M., Božunović, J., Nestorović Živković, J., Gašić, U., Milutinović, M., Dmitrović, S., Petrović, L., Filipović, B., Banjanac, T., Šiler, B., Anđelković, B., Dimitrijević, M.,& Mišić, D.. (2023). Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia
Belgrade: Faculty of Chemistry..
https://hdl.handle.net/21.15107/rcub_ibiss_6196

Aničić N, Matekalo D, Skorić M, Božunović J, Nestorović Živković J, Gašić U, Milutinović M, Dmitrović S, Petrović L, Filipović B, Banjanac T, Šiler B, Anđelković B, Dimitrijević M, Mišić D. Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme. in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia. 2023;:null-156.
https://hdl.handle.net/21.15107/rcub_ibiss_6196 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Božunović, Jelena, Nestorović Živković, Jasmina, Gašić, Uroš, Milutinović, Milica, Dmitrović, Slavica, Petrović, Luka, Filipović, Biljana, Banjanac, Tijana, Šiler, Branislav, Anđelković, Boban, Dimitrijević, Milena, Mišić, Danijela, "Insights into iridoid biosynthesis in Nepeta species (subfam. Nepetoidae, fam. Lamiaceae): Functional characterization of a key enzyme" in Biochemistry in Biotechnology: Serbian Biochemical Society, Twelfth Conference, International scientific meeting; 2023 Sep 21-23; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6196 .

TY  - CONF
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Gašić, Uroš
AU  - Dimitrijević, Milena
AU  - Anđelković, Boban
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Petrović, Luka
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6081
AB  - Within a large iridoids-lacking clade of Nepetoidae (Lamiaceae), the genus Nepeta stands out as an exclusive producer of these atypical monoterpenes with a unique stereochemistry. The genus Nepeta exhibits a high diversity of iridoids, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. Comparative metabolomics, transcriptomics, gene co-expression, and phylogenetic analysis were conducted on the trans,cis-NL-rich N. rtanjensis Diklić & Milojević, the cis,trans-NL-dominant N. sibirica L., and the iridoid-lacking N. nervosa Royle & Bentham, revealing significant structural and functional differences in their iridoid synthases (ISY). N. rtanjensis was found to have two orthologues of ISY (NrISY1 and NrISY2), while N. sibirica and N. nervosa each had only one ISY (NsISY and NnISY, respectively). Although the phylogenetic analysis grouped NrISY1, NsISY, and NnISY with non-active Family 1 isoforms, organ-specific expression highlighted NrISY2, NsISY, and NnISY as promising ISY candidates. In vitro assays confirmed their function and NrISY2 was the most efficient in converting 8-oxogeranial to cis,trans-nepetalactol. However, 3D modeling and molecular docking analyses suggested that NnISY may have a unique cyclase activity. Likely, under in vivo conditions, the NnISY lacks substrates to catalyze, as a result of the loss of upstream geraniol synthase (GES) activity, which results in the loss of iridoids in N. nervosa. Presumably, some of the downstream pathway genes (NEPSs and MLPLs), essential for the NL biosynthesis, also underwent structural and activity changes in this species. Our ongoing work is conducted towards testing these robust hypotheses and re-establishing iridoid biosynthesis in N. nervosa.
PB  - Davis: University of California
C3  - Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
T1  - Functional iridoid synthases from three Nepeta species with differing iridoid  profiles
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6081
ER  - 

@conference{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Gašić, Uroš and Dimitrijević, Milena and Anđelković, Boban and Božunović, Jelena and Milutinović, Milica and Petrović, Luka and Mišić, Danijela",
year = "2023",
abstract = "Within a large iridoids-lacking clade of Nepetoidae (Lamiaceae), the genus Nepeta stands out as an exclusive producer of these atypical monoterpenes with a unique stereochemistry. The genus Nepeta exhibits a high diversity of iridoids, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. Comparative metabolomics, transcriptomics, gene co-expression, and phylogenetic analysis were conducted on the trans,cis-NL-rich N. rtanjensis Diklić & Milojević, the cis,trans-NL-dominant N. sibirica L., and the iridoid-lacking N. nervosa Royle & Bentham, revealing significant structural and functional differences in their iridoid synthases (ISY). N. rtanjensis was found to have two orthologues of ISY (NrISY1 and NrISY2), while N. sibirica and N. nervosa each had only one ISY (NsISY and NnISY, respectively). Although the phylogenetic analysis grouped NrISY1, NsISY, and NnISY with non-active Family 1 isoforms, organ-specific expression highlighted NrISY2, NsISY, and NnISY as promising ISY candidates. In vitro assays confirmed their function and NrISY2 was the most efficient in converting 8-oxogeranial to cis,trans-nepetalactol. However, 3D modeling and molecular docking analyses suggested that NnISY may have a unique cyclase activity. Likely, under in vivo conditions, the NnISY lacks substrates to catalyze, as a result of the loss of upstream geraniol synthase (GES) activity, which results in the loss of iridoids in N. nervosa. Presumably, some of the downstream pathway genes (NEPSs and MLPLs), essential for the NL biosynthesis, also underwent structural and activity changes in this species. Our ongoing work is conducted towards testing these robust hypotheses and re-establishing iridoid biosynthesis in N. nervosa.",
publisher = "Davis: University of California",
journal = "Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA",
title = "Functional iridoid synthases from three Nepeta species with differing iridoid  profiles",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6081"
}

Aničić, N., Matekalo, D., Skorić, M., Nestorović Živković, J., Dmitrović, S., Gašić, U., Dimitrijević, M., Anđelković, B., Božunović, J., Milutinović, M., Petrović, L.,& Mišić, D.. (2023). Functional iridoid synthases from three Nepeta species with differing iridoid  profiles. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA
Davis: University of California., 80.
https://hdl.handle.net/21.15107/rcub_ibiss_6081

Aničić N, Matekalo D, Skorić M, Nestorović Živković J, Dmitrović S, Gašić U, Dimitrijević M, Anđelković B, Božunović J, Milutinović M, Petrović L, Mišić D. Functional iridoid synthases from three Nepeta species with differing iridoid  profiles. in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA. 2023;:80.
https://hdl.handle.net/21.15107/rcub_ibiss_6081 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Gašić, Uroš, Dimitrijević, Milena, Anđelković, Boban, Božunović, Jelena, Milutinović, Milica, Petrović, Luka, Mišić, Danijela, "Functional iridoid synthases from three Nepeta species with differing iridoid  profiles" in Conference Program: TERPNET 2023: The 15th International Meeting on the Biosynthesis,  Function, and Synthetic Biology of Isoprenoids; 2023 Jul 31-Aug 4; Davis, USA (2023):80,
https://hdl.handle.net/21.15107/rcub_ibiss_6081 .

TY  - JOUR
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Petrović, Luka
AU  - Dimitrijević, Milena
AU  - Anđelković, Boban
AU  - Mišić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6456
AB  - Iridoids, a class of atypical monoterpenes, exhibit exceptional diversity within the Nepeta genus (subfam. Nepetoidae, fam. Lamiaceae).The majority of these plants produce iridoids of the unique stereochemistry, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. By comparatively analyzing metabolomics, transcriptomics, gene co-expression, and phylogenetic data of the iridoid-producing N. rtanjensis Diklić & Milojević and iridoid-lacking N. nervosa Royle & Bentham, we presumed that one of the factors responsible for the absence of these compounds in N. nervosa is iridoid synthase (ISY). Two orthologues of ISY were mined from leaves transcriptome of N. rtanjensis (NrPRISE1 and NrPRISE2), while in N. nervosa only one (NnPRISE) was identified, and it was phylogenetically closer to the representatives of the Family 1 isoforms, designated as P5βRs. Organ-specific and MeJA-elicited profiling of iridoid content and co-expression analysis of IBG candidates, highlighted NrPRISE2 and NnPRISE as promising candidates for ISY orthologues, and their function was confirmed using in vitro assays with recombinant proteins, after heterologous expression of recombinant proteins in E. coli and their His-tag affinity purification. NrPRISE2 demonstrated ISY activity both in vitro and likely in planta, which was supported by the 3D modeling and molecular docking analysis, thus reclassification of NrPRISE2 to NrISY is accordingly recommended. NnPRISE also displays in vitro ISY-like activity, while its role under in vivo conditions was not here unambiguously confirmed. Most probably under in vivo conditions the NnPRISE lacks substrates to act upon, as a result of the loss of function of some of the upstream enzymes of the iridoid pathway. Our ongoing work is conducted towards re-establishing the biosynthesis of iridoids in N. nervosa.
PB  - Lausanne, Switzerland: Frontiers
T2  - Frontiers in Plant Science
T1  - Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)
VL  - 14
DO  - 10.3389/fpls.2023.1211453
SP  - 1211453
ER  - 

@article{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Gašić, Uroš and Nestorović Živković, Jasmina and Dmitrović, Slavica and Božunović, Jelena and Milutinović, Milica and Petrović, Luka and Dimitrijević, Milena and Anđelković, Boban and Mišić, Danijela",
year = "2023",
abstract = "Iridoids, a class of atypical monoterpenes, exhibit exceptional diversity within the Nepeta genus (subfam. Nepetoidae, fam. Lamiaceae).The majority of these plants produce iridoids of the unique stereochemistry, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. By comparatively analyzing metabolomics, transcriptomics, gene co-expression, and phylogenetic data of the iridoid-producing N. rtanjensis Diklić & Milojević and iridoid-lacking N. nervosa Royle & Bentham, we presumed that one of the factors responsible for the absence of these compounds in N. nervosa is iridoid synthase (ISY). Two orthologues of ISY were mined from leaves transcriptome of N. rtanjensis (NrPRISE1 and NrPRISE2), while in N. nervosa only one (NnPRISE) was identified, and it was phylogenetically closer to the representatives of the Family 1 isoforms, designated as P5βRs. Organ-specific and MeJA-elicited profiling of iridoid content and co-expression analysis of IBG candidates, highlighted NrPRISE2 and NnPRISE as promising candidates for ISY orthologues, and their function was confirmed using in vitro assays with recombinant proteins, after heterologous expression of recombinant proteins in E. coli and their His-tag affinity purification. NrPRISE2 demonstrated ISY activity both in vitro and likely in planta, which was supported by the 3D modeling and molecular docking analysis, thus reclassification of NrPRISE2 to NrISY is accordingly recommended. NnPRISE also displays in vitro ISY-like activity, while its role under in vivo conditions was not here unambiguously confirmed. Most probably under in vivo conditions the NnPRISE lacks substrates to act upon, as a result of the loss of function of some of the upstream enzymes of the iridoid pathway. Our ongoing work is conducted towards re-establishing the biosynthesis of iridoids in N. nervosa.",
publisher = "Lausanne, Switzerland: Frontiers",
journal = "Frontiers in Plant Science",
title = "Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)",
volume = "14",
doi = "10.3389/fpls.2023.1211453",
pages = "1211453"
}

Aničić, N., Matekalo, D., Skorić, M., Gašić, U., Nestorović Živković, J., Dmitrović, S., Božunović, J., Milutinović, M., Petrović, L., Dimitrijević, M., Anđelković, B.,& Mišić, D.. (2023). Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae). in Frontiers in Plant Science
Lausanne, Switzerland: Frontiers., 14, 1211453.
https://doi.org/10.3389/fpls.2023.1211453

Aničić N, Matekalo D, Skorić M, Gašić U, Nestorović Živković J, Dmitrović S, Božunović J, Milutinović M, Petrović L, Dimitrijević M, Anđelković B, Mišić D. Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae). in Frontiers in Plant Science. 2023;14:1211453.
doi:10.3389/fpls.2023.1211453 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Gašić, Uroš, Nestorović Živković, Jasmina, Dmitrović, Slavica, Božunović, Jelena, Milutinović, Milica, Petrović, Luka, Dimitrijević, Milena, Anđelković, Boban, Mišić, Danijela, "Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)" in Frontiers in Plant Science, 14 (2023):1211453,
https://doi.org/10.3389/fpls.2023.1211453 . .

TY  - CONF
AU  - Jovičić, Katarina
AU  - Đikanović, Vesna
AU  - Vranković, Jelena
AU  - Dimitrijević, Milena
PY  - 2023
UR  - https://www.oen-iad.org/congress2023/documents/conference%20book_44th_iad%20conference_2023.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5488
AB  - Pollution of freshwater ecosystems by discharges of untreated municipal wastewater represents a
major threat for aquatic organisms and thus human health. Serbia discharges wastewater without
previous treatment. In recent years, studies have shown that fatty acids are sensitive to alterations in
organism homeostasis, supporting their use for biomarkers of toxicant exposure. Fish species are an
important source of biologically valuable proteins, fats, fat-soluble vitamins, and n-3polyunsaturated
fatty acids. The purpose of this study was to investigate the diferences of fatty acid composition in
fish from urban sites of the Danube River. A total number of eight specimens of roach (Rutilus rutilus)
were collected from two localities - Višnjica, which is exposed to the discharge of the largest
wastewater collector in the city of Belgrade and Veliko Ratno Ostrvo, which is at the confluence of
Danube and Sava River, and is considered as control location. Fatty acid methyl esters (FAMEs) were
analyzed using gas chromatography–mass spectrometry (GC-MS). A total of 28 fatty acids were found
in the muscle tissue of the studied fish species. The results showed no significant differences in fatty
acid profile between polluted and control localities. All examined fish species contained essential
fatty acids (EPA, ALA, DHA) with DHA levels significantly higher in specimens from control site. The
proportion of total saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) was almost
the same while polyunsaturated fatty acids (PUFAs) were higher in control specimens. It is shown by
previous studies that the main reason of changes in PUFAs is that they could be oxidized under
oxidative stress conditions induced by metals or organic compounds. Even though anthropogenic
load exerted on Višnjica locality has not significantly affected the fish fatty acid profile, changes in
PUFAs indicated the presence of some xenobiotics in their environment.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - Impact of wastewater discharges on fatty acid profiles on roach (Rutilus rutilus) from Danube River, Belgrade
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5488
ER  - 

@conference{
author = "Jovičić, Katarina and Đikanović, Vesna and Vranković, Jelena and Dimitrijević, Milena",
year = "2023",
abstract = "Pollution of freshwater ecosystems by discharges of untreated municipal wastewater represents a
major threat for aquatic organisms and thus human health. Serbia discharges wastewater without
previous treatment. In recent years, studies have shown that fatty acids are sensitive to alterations in
organism homeostasis, supporting their use for biomarkers of toxicant exposure. Fish species are an
important source of biologically valuable proteins, fats, fat-soluble vitamins, and n-3polyunsaturated
fatty acids. The purpose of this study was to investigate the diferences of fatty acid composition in
fish from urban sites of the Danube River. A total number of eight specimens of roach (Rutilus rutilus)
were collected from two localities - Višnjica, which is exposed to the discharge of the largest
wastewater collector in the city of Belgrade and Veliko Ratno Ostrvo, which is at the confluence of
Danube and Sava River, and is considered as control location. Fatty acid methyl esters (FAMEs) were
analyzed using gas chromatography–mass spectrometry (GC-MS). A total of 28 fatty acids were found
in the muscle tissue of the studied fish species. The results showed no significant differences in fatty
acid profile between polluted and control localities. All examined fish species contained essential
fatty acids (EPA, ALA, DHA) with DHA levels significantly higher in specimens from control site. The
proportion of total saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) was almost
the same while polyunsaturated fatty acids (PUFAs) were higher in control specimens. It is shown by
previous studies that the main reason of changes in PUFAs is that they could be oxidized under
oxidative stress conditions induced by metals or organic compounds. Even though anthropogenic
load exerted on Višnjica locality has not significantly affected the fish fatty acid profile, changes in
PUFAs indicated the presence of some xenobiotics in their environment.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "Impact of wastewater discharges on fatty acid profiles on roach (Rutilus rutilus) from Danube River, Belgrade",
pages = "31",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5488"
}

Jovičić, K., Đikanović, V., Vranković, J.,& Dimitrijević, M.. (2023). Impact of wastewater discharges on fatty acid profiles on roach (Rutilus rutilus) from Danube River, Belgrade. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 31.
https://hdl.handle.net/21.15107/rcub_ibiss_5488

Jovičić K, Đikanović V, Vranković J, Dimitrijević M. Impact of wastewater discharges on fatty acid profiles on roach (Rutilus rutilus) from Danube River, Belgrade. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:31.
https://hdl.handle.net/21.15107/rcub_ibiss_5488 .

Jovičić, Katarina, Đikanović, Vesna, Vranković, Jelena, Dimitrijević, Milena, "Impact of wastewater discharges on fatty acid profiles on roach (Rutilus rutilus) from Danube River, Belgrade" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):31,
https://hdl.handle.net/21.15107/rcub_ibiss_5488 .

TY  - CONF
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Dmitrović, Slavica
AU  - Anđelković, Boban
AU  - Dimitrijević, Milena
AU  - Dragićević, Milan
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6563
AB  - Nepeta L. is the largest genus of the Lamiaceae family, and the exclusive representative of the
subfamily Nepetoidae which produces iridoids. However, the genus Nepeta comprises both taxa
producing iridoids (iridoid aglycones-IAs and/or iridoid glucosides-IGs), and those lacking iridoids.
Combining omics-guided approach and functional genomics in elucidating the genetic background
of the iridoid diversity within the genus Nepeta, we provide novel insights into molecular
mechanisms driving the iridoid biosynthesis, and thus into chemical evolution of iridoids within
the genus Nepeta. Multidisciplinary experimental approach is here explained on the example
of iridoid synthases (IS), functionally characterized by in vitro enzymatic assays using recombinant
proteins heterologously expressed in Escherichia coli. Functional ISs are expressed in both
iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa
L. (NnIS). N. nervosa possesses an iridoid biosynthetic platform, but inactive one, most likely due
to the “switching off” or “silencing” of some gene(s) upstream in the pathway, which suggests that
this species has lost the ability to produce iridoids during the evolution. Furthermore, comparative
iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs)
during the exposure of chemodiverse Nepeta taxa to environmental stresses (e.g. dehydration,
UV−B radiation, pathogens) or elicitors (MeJA), facilitated the explanation of regulatory mechanisms
determining the productivity of plants, as well as of factors responsible for the presence/
absence of iridoids or specific groups of iridoids (IAs and IGs), in these plants.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights
SP  - 136
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6563
ER  - 

@conference{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Božunović, Jelena and Nestorović Živković, Jasmina and Gašić, Uroš and Milutinović, Milica and Dmitrović, Slavica and Anđelković, Boban and Dimitrijević, Milena and Dragićević, Milan and Mišić, Danijela",
year = "2022",
abstract = "Nepeta L. is the largest genus of the Lamiaceae family, and the exclusive representative of the
subfamily Nepetoidae which produces iridoids. However, the genus Nepeta comprises both taxa
producing iridoids (iridoid aglycones-IAs and/or iridoid glucosides-IGs), and those lacking iridoids.
Combining omics-guided approach and functional genomics in elucidating the genetic background
of the iridoid diversity within the genus Nepeta, we provide novel insights into molecular
mechanisms driving the iridoid biosynthesis, and thus into chemical evolution of iridoids within
the genus Nepeta. Multidisciplinary experimental approach is here explained on the example
of iridoid synthases (IS), functionally characterized by in vitro enzymatic assays using recombinant
proteins heterologously expressed in Escherichia coli. Functional ISs are expressed in both
iridoid-producing N. rtanjensis (NrIS2) and N. sibirica L. (NsIS), as well as in iridoid-lacking N. nervosa
L. (NnIS). N. nervosa possesses an iridoid biosynthetic platform, but inactive one, most likely due
to the “switching off” or “silencing” of some gene(s) upstream in the pathway, which suggests that
this species has lost the ability to produce iridoids during the evolution. Furthermore, comparative
iridoid profiling and co-expression analysis of biosynthetic genes and transcription factors (TFs)
during the exposure of chemodiverse Nepeta taxa to environmental stresses (e.g. dehydration,
UV−B radiation, pathogens) or elicitors (MeJA), facilitated the explanation of regulatory mechanisms
determining the productivity of plants, as well as of factors responsible for the presence/
absence of iridoids or specific groups of iridoids (IAs and IGs), in these plants.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights",
pages = "136",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6563"
}

Aničić, N., Matekalo, D., Skorić, M., Božunović, J., Nestorović Živković, J., Gašić, U., Milutinović, M., Dmitrović, S., Anđelković, B., Dimitrijević, M., Dragićević, M.,& Mišić, D.. (2022). Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 136.
https://hdl.handle.net/21.15107/rcub_ibiss_6563

Aničić N, Matekalo D, Skorić M, Božunović J, Nestorović Živković J, Gašić U, Milutinović M, Dmitrović S, Anđelković B, Dimitrijević M, Dragićević M, Mišić D. Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:136.
https://hdl.handle.net/21.15107/rcub_ibiss_6563 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Božunović, Jelena, Nestorović Živković, Jasmina, Gašić, Uroš, Milutinović, Milica, Dmitrović, Slavica, Anđelković, Boban, Dimitrijević, Milena, Dragićević, Milan, Mišić, Danijela, "Progress in disentangling the diversity of iridoids within the genus Nepeta: surprising biosynthetic and evolutionary insights" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):136,
https://hdl.handle.net/21.15107/rcub_ibiss_6563 .

TY  - JOUR
AU  - Tasić, Danica
AU  - Opačić, Miloš
AU  - Kovačević, Sanja
AU  - Nikolić-Kokić, Aleksandra
AU  - Dimitrijević, Milena
AU  - Nikolić, Dušan
AU  - Vojnović-Milutinović, Danijela
AU  - Blagojević, Duško
AU  - Đorđević, Ana
AU  - Brkljačić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5025
AB  - The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys
are still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression
of metabolic disturbances in the kidney. We determined protein level of copper transporters,
chaperones, and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes
function in the kidneys of male Wistar rats subjected to 20% liquid fructose supplementation
and/or chronic stress. Liquid fructose supplementation increased level of copper chaperone of
superoxide dismutase and decreased metallothionein level, while rendering the level of copper
importer and copper chaperones involved in copper delivery to mitochondria and trans Golgi
network unaffected. Stress had no effect on renal copper metabolism. The activity and expression
of renal antioxidant enzymes remained unaltered in all experimental groups. In conclusion, fructose,
independently of stress, decreased renal copper level, and modulated renal copper metabolism
as to preserve vital cellular function including mitochondrial energy production and antioxidative
defense, at the expense of intracellular copper storage.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function
IS  - 16
VL  - 23
DO  - 10.3390/ijms23169023
SP  - 9023
ER  - 

@article{
author = "Tasić, Danica and Opačić, Miloš and Kovačević, Sanja and Nikolić-Kokić, Aleksandra and Dimitrijević, Milena and Nikolić, Dušan and Vojnović-Milutinović, Danijela and Blagojević, Duško and Đorđević, Ana and Brkljačić, Jelena",
year = "2022",
abstract = "The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys
are still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression
of metabolic disturbances in the kidney. We determined protein level of copper transporters,
chaperones, and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes
function in the kidneys of male Wistar rats subjected to 20% liquid fructose supplementation
and/or chronic stress. Liquid fructose supplementation increased level of copper chaperone of
superoxide dismutase and decreased metallothionein level, while rendering the level of copper
importer and copper chaperones involved in copper delivery to mitochondria and trans Golgi
network unaffected. Stress had no effect on renal copper metabolism. The activity and expression
of renal antioxidant enzymes remained unaltered in all experimental groups. In conclusion, fructose,
independently of stress, decreased renal copper level, and modulated renal copper metabolism
as to preserve vital cellular function including mitochondrial energy production and antioxidative
defense, at the expense of intracellular copper storage.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function",
number = "16",
volume = "23",
doi = "10.3390/ijms23169023",
pages = "9023"
}

Tasić, D., Opačić, M., Kovačević, S., Nikolić-Kokić, A., Dimitrijević, M., Nikolić, D., Vojnović-Milutinović, D., Blagojević, D., Đorđević, A.,& Brkljačić, J.. (2022). Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences
Basel: MDPI., 23(16), 9023.
https://doi.org/10.3390/ijms23169023

Tasić D, Opačić M, Kovačević S, Nikolić-Kokić A, Dimitrijević M, Nikolić D, Vojnović-Milutinović D, Blagojević D, Đorđević A, Brkljačić J. Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences. 2022;23(16):9023.
doi:10.3390/ijms23169023 .

Tasić, Danica, Opačić, Miloš, Kovačević, Sanja, Nikolić-Kokić, Aleksandra, Dimitrijević, Milena, Nikolić, Dušan, Vojnović-Milutinović, Danijela, Blagojević, Duško, Đorđević, Ana, Brkljačić, Jelena, "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function" in International Journal of Molecular Sciences, 23, no. 16 (2022):9023,
https://doi.org/10.3390/ijms23169023 . .

TY  - JOUR
AU  - Aničić, Neda
AU  - Gašić, Uroš
AU  - Lu, Feng
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Jevtić, Bojan
AU  - Dimitrijević, Milena
AU  - Anđelković, Boban
AU  - Skorić, Marijana
AU  - Nestorović Živković, Jasmina
AU  - Mao, Yingle
AU  - Liu, Jia
AU  - Tang, Chunping
AU  - Soković, Marina
AU  - Ye, Yang
AU  - Mišić, Danijela
PY  - 2021
UR  - https://doi.org/10.3390/ph14050414
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4249
AB  - Two Balkan Peninsula endemics, Nepeta rtanjensis and N. argolica subsp. argolica, both characterized by specialized metabolite profiles predominated by iridoids and phenolics, are differentiated according to the stereochemistry of major iridoid aglycone nepetalactone (NL). For the first time, the present study provides a comparative analysis of antimicrobial and immunomodulating activities of the two Nepeta species and their major iridoids isolated from natural sources—cis,transNL, trans,cis-NL, and 1,5,9-epideoxyloganic acid (1,5,9-eDLA), as well as of phenolic acid rosmarinic acid (RA). Methanol extracts and pure iridoids displayed excellent antimicrobial activity against eight strains of bacteria and seven strains of fungi. They were especially potent against food-borne pathogens such as L. monocytogenes, E. coli, S. aureus, Penicillium sp., and Aspergillus sp. Targeted iridoids were efficient agents in preventing biofilm formation of resistant P. aeruginosa strain, and they displayed additive antimicrobial interaction. Iridoids are, to a great extent, responsible for the prominent antimicrobial activities of the two Nepeta species, although are probably minor contributors to the moderate immunomodulatory effects. The analyzed iridoids and RA, individually or in mixtures, have the potential to be used in the pharmaceutical industry as potent antimicrobials, and in the food industry to increase the shelf life and safety of food products.
PB  - MDPI AG
T2  - Pharmaceuticals
T1  - Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources
IS  - 5
VL  - 14
DO  - 10.3390/ph14050414
SP  - 414
ER  - 

@article{
author = "Aničić, Neda and Gašić, Uroš and Lu, Feng and Ćirić, Ana and Ivanov, Marija and Jevtić, Bojan and Dimitrijević, Milena and Anđelković, Boban and Skorić, Marijana and Nestorović Živković, Jasmina and Mao, Yingle and Liu, Jia and Tang, Chunping and Soković, Marina and Ye, Yang and Mišić, Danijela",
year = "2021",
abstract = "Two Balkan Peninsula endemics, Nepeta rtanjensis and N. argolica subsp. argolica, both characterized by specialized metabolite profiles predominated by iridoids and phenolics, are differentiated according to the stereochemistry of major iridoid aglycone nepetalactone (NL). For the first time, the present study provides a comparative analysis of antimicrobial and immunomodulating activities of the two Nepeta species and their major iridoids isolated from natural sources—cis,transNL, trans,cis-NL, and 1,5,9-epideoxyloganic acid (1,5,9-eDLA), as well as of phenolic acid rosmarinic acid (RA). Methanol extracts and pure iridoids displayed excellent antimicrobial activity against eight strains of bacteria and seven strains of fungi. They were especially potent against food-borne pathogens such as L. monocytogenes, E. coli, S. aureus, Penicillium sp., and Aspergillus sp. Targeted iridoids were efficient agents in preventing biofilm formation of resistant P. aeruginosa strain, and they displayed additive antimicrobial interaction. Iridoids are, to a great extent, responsible for the prominent antimicrobial activities of the two Nepeta species, although are probably minor contributors to the moderate immunomodulatory effects. The analyzed iridoids and RA, individually or in mixtures, have the potential to be used in the pharmaceutical industry as potent antimicrobials, and in the food industry to increase the shelf life and safety of food products.",
publisher = "MDPI AG",
journal = "Pharmaceuticals",
title = "Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources",
number = "5",
volume = "14",
doi = "10.3390/ph14050414",
pages = "414"
}

Aničić, N., Gašić, U., Lu, F., Ćirić, A., Ivanov, M., Jevtić, B., Dimitrijević, M., Anđelković, B., Skorić, M., Nestorović Živković, J., Mao, Y., Liu, J., Tang, C., Soković, M., Ye, Y.,& Mišić, D.. (2021). Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources. in Pharmaceuticals
MDPI AG., 14(5), 414.
https://doi.org/10.3390/ph14050414

Aničić N, Gašić U, Lu F, Ćirić A, Ivanov M, Jevtić B, Dimitrijević M, Anđelković B, Skorić M, Nestorović Živković J, Mao Y, Liu J, Tang C, Soković M, Ye Y, Mišić D. Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources. in Pharmaceuticals. 2021;14(5):414.
doi:10.3390/ph14050414 .

Aničić, Neda, Gašić, Uroš, Lu, Feng, Ćirić, Ana, Ivanov, Marija, Jevtić, Bojan, Dimitrijević, Milena, Anđelković, Boban, Skorić, Marijana, Nestorović Živković, Jasmina, Mao, Yingle, Liu, Jia, Tang, Chunping, Soković, Marina, Ye, Yang, Mišić, Danijela, "Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources" in Pharmaceuticals, 14, no. 5 (2021):414,
https://doi.org/10.3390/ph14050414 . .

TY  - JOUR
AU  - Korać Jačić, Jelena
AU  - Nikolić, Ljiljana
AU  - Stanković, Dalibor M.
AU  - Opačić, Miloš
AU  - Dimitrijević, Milena
AU  - Savić, Danijela
AU  - Grgurić Šipka, Sanja
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3597
AB  - Upon release in response to stress, epinephrine (Epi) may interact with labile iron pool in human plasma with potentially important (patho)physiological consequences. We have shown that Epi and Fe3+ build stable 1:1 high-spin bidentate complex at physiological pH, and that Epi does not undergo degradation in the presence of iron. However, the interactions of Epi with the more soluble Fe2+, and the impact of iron on biological activity of Epi are still not known. Herein we showed that Epi and Fe2+ build colorless complex which is stable under anaerobic conditions. In the presence of O2, Epi promoted the oxidation of Fe2+ and the formation of Epi-Fe3+ complex. Cyclic voltammetry showed that mid-point potential of Epi-Fe2+ complex is very low (-582 mV vs. standard hydrogen electrode), which explains catalyzed oxidation of Fe2+. Next, we examined the impact of iron binding on biological performance of Epi using patch clamping in cell culture with constitutive expression of adrenergic receptors. Epi alone evoked an increase of outward currents, whereas Epi in the complex with Fe3+ did not. This implies that the binding of Epi to adrenergic receptors and their activation is prevented by the formation of complex with iron. Pro-oxidative activity of Epi-Fe2+ complex may represent a link between chronic stress and cardiovascular problems. On the other hand, labile iron could serve as a modulator of biological activity of ligands. Such interactions may be important in human pathologies that are related to iron overload or deficiency.
T2  - Free Radical Biology and Medicine
T1  - Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.
VL  - 148
DO  - 10.1016/j.freeradbiomed.2020.01.001
SP  - 123
EP  - 127
ER  - 

@article{
author = "Korać Jačić, Jelena and Nikolić, Ljiljana and Stanković, Dalibor M. and Opačić, Miloš and Dimitrijević, Milena and Savić, Danijela and Grgurić Šipka, Sanja and Spasojević, Ivan and Bogdanović Pristov, Jelena",
year = "2020",
abstract = "Upon release in response to stress, epinephrine (Epi) may interact with labile iron pool in human plasma with potentially important (patho)physiological consequences. We have shown that Epi and Fe3+ build stable 1:1 high-spin bidentate complex at physiological pH, and that Epi does not undergo degradation in the presence of iron. However, the interactions of Epi with the more soluble Fe2+, and the impact of iron on biological activity of Epi are still not known. Herein we showed that Epi and Fe2+ build colorless complex which is stable under anaerobic conditions. In the presence of O2, Epi promoted the oxidation of Fe2+ and the formation of Epi-Fe3+ complex. Cyclic voltammetry showed that mid-point potential of Epi-Fe2+ complex is very low (-582 mV vs. standard hydrogen electrode), which explains catalyzed oxidation of Fe2+. Next, we examined the impact of iron binding on biological performance of Epi using patch clamping in cell culture with constitutive expression of adrenergic receptors. Epi alone evoked an increase of outward currents, whereas Epi in the complex with Fe3+ did not. This implies that the binding of Epi to adrenergic receptors and their activation is prevented by the formation of complex with iron. Pro-oxidative activity of Epi-Fe2+ complex may represent a link between chronic stress and cardiovascular problems. On the other hand, labile iron could serve as a modulator of biological activity of ligands. Such interactions may be important in human pathologies that are related to iron overload or deficiency.",
journal = "Free Radical Biology and Medicine",
title = "Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.",
volume = "148",
doi = "10.1016/j.freeradbiomed.2020.01.001",
pages = "123-127"
}

Korać Jačić, J., Nikolić, L., Stanković, D. M., Opačić, M., Dimitrijević, M., Savić, D., Grgurić Šipka, S., Spasojević, I.,& Bogdanović Pristov, J.. (2020). Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.. in Free Radical Biology and Medicine, 148, 123-127.
https://doi.org/10.1016/j.freeradbiomed.2020.01.001

Korać Jačić J, Nikolić L, Stanković DM, Opačić M, Dimitrijević M, Savić D, Grgurić Šipka S, Spasojević I, Bogdanović Pristov J. Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.. in Free Radical Biology and Medicine. 2020;148:123-127.
doi:10.1016/j.freeradbiomed.2020.01.001 .

Korać Jačić, Jelena, Nikolić, Ljiljana, Stanković, Dalibor M., Opačić, Miloš, Dimitrijević, Milena, Savić, Danijela, Grgurić Šipka, Sanja, Spasojević, Ivan, Bogdanović Pristov, Jelena, "Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors." in Free Radical Biology and Medicine, 148 (2020):123-127,
https://doi.org/10.1016/j.freeradbiomed.2020.01.001 . .

TY  - CONF
AU  - Mišić, Danijela
AU  - Aničić, Neda
AU  - Nestorović Živković, Jasmina
AU  - Dimitrijević, Milena
AU  - Dumanović, Jelena
AU  - Šiler, Branislav
AU  - Gašić, Uroš
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4354
AB  - Endemiti Balkanskog poluostrva, vrste Nepeta ernesti-mayeri Diklić & Nikolić, N.
parnassica Heldr. & Sart., N. rtanjensis Diklić & Milojević, i N. sibthorpii Benth.,
pripadaju sect. Pycnonepeta Benth. (fam. Lamiaceae). Osim što pokazuju visok stepen
sličnosti u pogledu morfoloških i ekofizioloških karakteristika, ove taksonomski bliske
vrste odlikuje sličan sadržaj glavnih grupa sekundarnih metabolita, terpenoida i fenolnih
jedinjenja. Međutim, analizirane vrste roda Nepeta se razlikuju u pogledu kvalitativnog
sastava 7S diasteroizomera nepetalaktona1
, što ovo jedinjenje čini diskriminativnim
hemotaksonomskim markerom u okviru ove taksonomske grupe. UHPLC–
LTQ/orbitrap/MS metoda je korišćena za profilisanje fenolnih jedinjenja odabranih vrsta
roda Nepeta, nakon čega je UHPLC/DAD/(+/−)HESI–MS/MS analizom izvršena
kvantifikacija 6 dominantnih derivata hidroksicinamične kiseline i 3 nepetalaktona.
Prisustvo cis,trans–nepetalaktona kao dominantnog diasteroizomera utvrđeno je kod
vrsta N. ernesti-mayeri i N. sibthorpii, dok je trans,cis-nepetalakton najzastupljeniji kod
vrsta N. rtanjensis i N. parnassica. Kod vrsta N. rtanjensis i N. parnassica zabeležen je
visoki sadržaj dehidronepetalaktona. Metanolni ekstrakti izdanaka N. rtanjensis i N.
sibthorpii podvrgnuti su semipreparativnoj HPLC/DAD analizi i frakcionisanju, s ciljem
izolacije cis,trans-, trans,cis-nepetalaktona i dehidronepetalaktona čija je identifikacija
potvrđena UHPLC/(+/−)HESI–MS/MS i GC-MS analizama.
PB  - Belgrade: Serbian Biological Society
C3  - Drugi kongres biologa Srbije, Kladovo 25-30.09.2018., knjiga sažetaka
T1  - Hemotaksonomski markeri vrsta Nepeta sibthorpii Benth. kompleksa (sect. Pycnonepeta Benth.)
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4354
ER  - 

@conference{
author = "Mišić, Danijela and Aničić, Neda and Nestorović Živković, Jasmina and Dimitrijević, Milena and Dumanović, Jelena and Šiler, Branislav and Gašić, Uroš and Matekalo, Dragana and Skorić, Marijana",
year = "2018",
abstract = "Endemiti Balkanskog poluostrva, vrste Nepeta ernesti-mayeri Diklić & Nikolić, N.
parnassica Heldr. & Sart., N. rtanjensis Diklić & Milojević, i N. sibthorpii Benth.,
pripadaju sect. Pycnonepeta Benth. (fam. Lamiaceae). Osim što pokazuju visok stepen
sličnosti u pogledu morfoloških i ekofizioloških karakteristika, ove taksonomski bliske
vrste odlikuje sličan sadržaj glavnih grupa sekundarnih metabolita, terpenoida i fenolnih
jedinjenja. Međutim, analizirane vrste roda Nepeta se razlikuju u pogledu kvalitativnog
sastava 7S diasteroizomera nepetalaktona1
, što ovo jedinjenje čini diskriminativnim
hemotaksonomskim markerom u okviru ove taksonomske grupe. UHPLC–
LTQ/orbitrap/MS metoda je korišćena za profilisanje fenolnih jedinjenja odabranih vrsta
roda Nepeta, nakon čega je UHPLC/DAD/(+/−)HESI–MS/MS analizom izvršena
kvantifikacija 6 dominantnih derivata hidroksicinamične kiseline i 3 nepetalaktona.
Prisustvo cis,trans–nepetalaktona kao dominantnog diasteroizomera utvrđeno je kod
vrsta N. ernesti-mayeri i N. sibthorpii, dok je trans,cis-nepetalakton najzastupljeniji kod
vrsta N. rtanjensis i N. parnassica. Kod vrsta N. rtanjensis i N. parnassica zabeležen je
visoki sadržaj dehidronepetalaktona. Metanolni ekstrakti izdanaka N. rtanjensis i N.
sibthorpii podvrgnuti su semipreparativnoj HPLC/DAD analizi i frakcionisanju, s ciljem
izolacije cis,trans-, trans,cis-nepetalaktona i dehidronepetalaktona čija je identifikacija
potvrđena UHPLC/(+/−)HESI–MS/MS i GC-MS analizama.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Drugi kongres biologa Srbije, Kladovo 25-30.09.2018., knjiga sažetaka",
title = "Hemotaksonomski markeri vrsta Nepeta sibthorpii Benth. kompleksa (sect. Pycnonepeta Benth.)",
pages = "57",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4354"
}

Mišić, D., Aničić, N., Nestorović Živković, J., Dimitrijević, M., Dumanović, J., Šiler, B., Gašić, U., Matekalo, D.,& Skorić, M.. (2018). Hemotaksonomski markeri vrsta Nepeta sibthorpii Benth. kompleksa (sect. Pycnonepeta Benth.). in Drugi kongres biologa Srbije, Kladovo 25-30.09.2018., knjiga sažetaka
Belgrade: Serbian Biological Society., 57.
https://hdl.handle.net/21.15107/rcub_ibiss_4354

Mišić D, Aničić N, Nestorović Živković J, Dimitrijević M, Dumanović J, Šiler B, Gašić U, Matekalo D, Skorić M. Hemotaksonomski markeri vrsta Nepeta sibthorpii Benth. kompleksa (sect. Pycnonepeta Benth.). in Drugi kongres biologa Srbije, Kladovo 25-30.09.2018., knjiga sažetaka. 2018;:57.
https://hdl.handle.net/21.15107/rcub_ibiss_4354 .

Mišić, Danijela, Aničić, Neda, Nestorović Živković, Jasmina, Dimitrijević, Milena, Dumanović, Jelena, Šiler, Branislav, Gašić, Uroš, Matekalo, Dragana, Skorić, Marijana, "Hemotaksonomski markeri vrsta Nepeta sibthorpii Benth. kompleksa (sect. Pycnonepeta Benth.)" in Drugi kongres biologa Srbije, Kladovo 25-30.09.2018., knjiga sažetaka (2018):57,
https://hdl.handle.net/21.15107/rcub_ibiss_4354 .