TY  - JOUR
AU  - Opačić, Miloš
AU  - Ristić, Aleksandar J.
AU  - Sokić, Dragoslav
AU  - Baščarević, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Zorović, Maja
AU  - Živin, Marko
AU  - Šelih, Vid Simon
AU  - Spasojević, Ivan
AU  - Savić, Danijela
PY  - 2021
UR  - https://onlinelibrary.wiley.com/doi/10.1002/brb3.1986
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4403
AB  - Introduction Disruption of copper homeostasis and dysfunction of mitochondria have been documented in sclerotic hippocampi (HS) of patients with mesial temporal lobe epilepsy (mTLE). However, a potential link between these pathological changes has not been tackled so far. Herein, we analyzed regional distribution of neuron somata density, copper concentration, and the activity of cytochrome c oxidase (CCO), a component of mitochondrial electron transport chain and copper-containing metalloprotein, in HS. Methods Histochemical staining and laser ablation inductively coupled plasma mass spectrometry were carried out to construct comparable maps of these parameters in coronal sections of hippocampi of 3 mTLE-HS patients and 3 control subjects. Results Copper levels were decreased in all regions of HS with pyramidal neuron somata. CCO activity was significantly reduced in stratum pyramidale (PY) 1 and cornu Ammonis field 4, the two regions with significant reduction in neuron somata density. CCO activity was also lower in layers that contain apical dendrites of pyramidal neurons and mossy fibers. It appears that copper deficiency in PY2 and PY3 comes before CCO activity reduction and neuronal loss. A strong positive correlation was found between neuron density, Cu concentration, and CCO activity. Conclusions Presented results imply that pathological alterations in Cu and energy metabolism could be involved in the development of HS. A limitation of this study was the relatively small number of patients. However, presented results underline copper deficiency as a component of pathological mechanisms of epilepsy and warrant further investigation of cuproproteins and members of copper transport machinery.
T2  - Brain and Behavior
T1  - Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients
IS  - 2
VL  - 11
DO  - 10.1002/brb3.1986
SP  - e01986
ER  - 

@article{
author = "Opačić, Miloš and Ristić, Aleksandar J. and Sokić, Dragoslav and Baščarević, Vladimir and Raičević, Savo and Savić, Slobodan and Zorović, Maja and Živin, Marko and Šelih, Vid Simon and Spasojević, Ivan and Savić, Danijela",
year = "2021",
abstract = "Introduction Disruption of copper homeostasis and dysfunction of mitochondria have been documented in sclerotic hippocampi (HS) of patients with mesial temporal lobe epilepsy (mTLE). However, a potential link between these pathological changes has not been tackled so far. Herein, we analyzed regional distribution of neuron somata density, copper concentration, and the activity of cytochrome c oxidase (CCO), a component of mitochondrial electron transport chain and copper-containing metalloprotein, in HS. Methods Histochemical staining and laser ablation inductively coupled plasma mass spectrometry were carried out to construct comparable maps of these parameters in coronal sections of hippocampi of 3 mTLE-HS patients and 3 control subjects. Results Copper levels were decreased in all regions of HS with pyramidal neuron somata. CCO activity was significantly reduced in stratum pyramidale (PY) 1 and cornu Ammonis field 4, the two regions with significant reduction in neuron somata density. CCO activity was also lower in layers that contain apical dendrites of pyramidal neurons and mossy fibers. It appears that copper deficiency in PY2 and PY3 comes before CCO activity reduction and neuronal loss. A strong positive correlation was found between neuron density, Cu concentration, and CCO activity. Conclusions Presented results imply that pathological alterations in Cu and energy metabolism could be involved in the development of HS. A limitation of this study was the relatively small number of patients. However, presented results underline copper deficiency as a component of pathological mechanisms of epilepsy and warrant further investigation of cuproproteins and members of copper transport machinery.",
journal = "Brain and Behavior",
title = "Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients",
number = "2",
volume = "11",
doi = "10.1002/brb3.1986",
pages = "e01986"
}

Opačić, M., Ristić, A. J., Sokić, D., Baščarević, V., Raičević, S., Savić, S., Zorović, M., Živin, M., Šelih, V. S., Spasojević, I.,& Savić, D.. (2021). Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients. in Brain and Behavior, 11(2), e01986.
https://doi.org/10.1002/brb3.1986

Opačić M, Ristić AJ, Sokić D, Baščarević V, Raičević S, Savić S, Zorović M, Živin M, Šelih VS, Spasojević I, Savić D. Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients. in Brain and Behavior. 2021;11(2):e01986.
doi:10.1002/brb3.1986 .

Opačić, Miloš, Ristić, Aleksandar J., Sokić, Dragoslav, Baščarević, Vladimir, Raičević, Savo, Savić, Slobodan, Zorović, Maja, Živin, Marko, Šelih, Vid Simon, Spasojević, Ivan, Savić, Danijela, "Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients" in Brain and Behavior, 11, no. 2 (2021):e01986,
https://doi.org/10.1002/brb3.1986 . .

TY  - JOUR
AU  - Korać Jačić, Jelena
AU  - Nikolić, Ljiljana
AU  - Stanković, Dalibor M.
AU  - Opačić, Miloš
AU  - Dimitrijević, Milena
AU  - Savić, Danijela
AU  - Grgurić Šipka, Sanja
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3597
AB  - Upon release in response to stress, epinephrine (Epi) may interact with labile iron pool in human plasma with potentially important (patho)physiological consequences. We have shown that Epi and Fe3+ build stable 1:1 high-spin bidentate complex at physiological pH, and that Epi does not undergo degradation in the presence of iron. However, the interactions of Epi with the more soluble Fe2+, and the impact of iron on biological activity of Epi are still not known. Herein we showed that Epi and Fe2+ build colorless complex which is stable under anaerobic conditions. In the presence of O2, Epi promoted the oxidation of Fe2+ and the formation of Epi-Fe3+ complex. Cyclic voltammetry showed that mid-point potential of Epi-Fe2+ complex is very low (-582 mV vs. standard hydrogen electrode), which explains catalyzed oxidation of Fe2+. Next, we examined the impact of iron binding on biological performance of Epi using patch clamping in cell culture with constitutive expression of adrenergic receptors. Epi alone evoked an increase of outward currents, whereas Epi in the complex with Fe3+ did not. This implies that the binding of Epi to adrenergic receptors and their activation is prevented by the formation of complex with iron. Pro-oxidative activity of Epi-Fe2+ complex may represent a link between chronic stress and cardiovascular problems. On the other hand, labile iron could serve as a modulator of biological activity of ligands. Such interactions may be important in human pathologies that are related to iron overload or deficiency.
T2  - Free Radical Biology and Medicine
T1  - Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.
VL  - 148
DO  - 10.1016/j.freeradbiomed.2020.01.001
SP  - 123
EP  - 127
ER  - 

@article{
author = "Korać Jačić, Jelena and Nikolić, Ljiljana and Stanković, Dalibor M. and Opačić, Miloš and Dimitrijević, Milena and Savić, Danijela and Grgurić Šipka, Sanja and Spasojević, Ivan and Bogdanović Pristov, Jelena",
year = "2020",
abstract = "Upon release in response to stress, epinephrine (Epi) may interact with labile iron pool in human plasma with potentially important (patho)physiological consequences. We have shown that Epi and Fe3+ build stable 1:1 high-spin bidentate complex at physiological pH, and that Epi does not undergo degradation in the presence of iron. However, the interactions of Epi with the more soluble Fe2+, and the impact of iron on biological activity of Epi are still not known. Herein we showed that Epi and Fe2+ build colorless complex which is stable under anaerobic conditions. In the presence of O2, Epi promoted the oxidation of Fe2+ and the formation of Epi-Fe3+ complex. Cyclic voltammetry showed that mid-point potential of Epi-Fe2+ complex is very low (-582 mV vs. standard hydrogen electrode), which explains catalyzed oxidation of Fe2+. Next, we examined the impact of iron binding on biological performance of Epi using patch clamping in cell culture with constitutive expression of adrenergic receptors. Epi alone evoked an increase of outward currents, whereas Epi in the complex with Fe3+ did not. This implies that the binding of Epi to adrenergic receptors and their activation is prevented by the formation of complex with iron. Pro-oxidative activity of Epi-Fe2+ complex may represent a link between chronic stress and cardiovascular problems. On the other hand, labile iron could serve as a modulator of biological activity of ligands. Such interactions may be important in human pathologies that are related to iron overload or deficiency.",
journal = "Free Radical Biology and Medicine",
title = "Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.",
volume = "148",
doi = "10.1016/j.freeradbiomed.2020.01.001",
pages = "123-127"
}

Korać Jačić, J., Nikolić, L., Stanković, D. M., Opačić, M., Dimitrijević, M., Savić, D., Grgurić Šipka, S., Spasojević, I.,& Bogdanović Pristov, J.. (2020). Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.. in Free Radical Biology and Medicine, 148, 123-127.
https://doi.org/10.1016/j.freeradbiomed.2020.01.001

Korać Jačić J, Nikolić L, Stanković DM, Opačić M, Dimitrijević M, Savić D, Grgurić Šipka S, Spasojević I, Bogdanović Pristov J. Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.. in Free Radical Biology and Medicine. 2020;148:123-127.
doi:10.1016/j.freeradbiomed.2020.01.001 .

Korać Jačić, Jelena, Nikolić, Ljiljana, Stanković, Dalibor M., Opačić, Miloš, Dimitrijević, Milena, Savić, Danijela, Grgurić Šipka, Sanja, Spasojević, Ivan, Bogdanović Pristov, Jelena, "Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors." in Free Radical Biology and Medicine, 148 (2020):123-127,
https://doi.org/10.1016/j.freeradbiomed.2020.01.001 . .

TY  - CONF
AU  - Savić, Danijela
AU  - Opačić, Miloš
AU  - Brkljačić, Jelena
AU  - Ristić, Aleksandar
AU  - Sokić, Dragoslav
AU  - Baščarević, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Zorović, Maja
AU  - Živin, Marko
AU  - Šelih, Vid Simon
AU  - Spasić, Snežana
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5882
AB  - More and more studies are identifying the regulation of metal homeostasis as one of the key points of central nervous system’s
well-being. Epilepsy is a particularly interesting neurological condition when viewed in terms of the correlation between the
amount of metals and the development of a seizure. This lecture will present contribution of our group to the field of metal
biology in epilepsy by mapping brain metals in sclerotic hippocampus resected from drug resistant mesial temporal lobe
epilepsy (mTLE) patients as surgical therapeutic approach. Direct insight into this epileptogenic area, by two powerful techniques,
optical emission and mass spectrometry, has led us to investigation of copper turnover. Namely, among the examined metals,
we found the deficiency of copper in sclerotic hippocampus on two levels: (i) in whole structure (ii) and locally in the areas of
neuronal loss, with significant correlation between copper concentration and neuron density. Furthermore, analysis of copper
metalloproteins showed: (i) significant increase or decrease in levels of protein that is participating in copper transport into
the cell (CTR1) depending on the degree of hippocampal neuronal loss; (ii) and lower activity of an enzyme in which copper
is part of the active site, cytochrome c oxidase, in sclerotic hippocampi of patients compared to control tissue. In our further
investigations it remained to be determined whether changes in copper concentrations and copper metalloproteins are causal
to pathology of mTLE or they represent epiphenomenon.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - The importance of copper in pathology of mesial temporal lobe epilepsy
SP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5882
ER  - 

@conference{
author = "Savić, Danijela and Opačić, Miloš and Brkljačić, Jelena and Ristić, Aleksandar and Sokić, Dragoslav and Baščarević, Vladimir and Raičević, Savo and Savić, Slobodan and Zorović, Maja and Živin, Marko and Šelih, Vid Simon and Spasić, Snežana and Spasojević, Ivan",
year = "2019",
abstract = "More and more studies are identifying the regulation of metal homeostasis as one of the key points of central nervous system’s
well-being. Epilepsy is a particularly interesting neurological condition when viewed in terms of the correlation between the
amount of metals and the development of a seizure. This lecture will present contribution of our group to the field of metal
biology in epilepsy by mapping brain metals in sclerotic hippocampus resected from drug resistant mesial temporal lobe
epilepsy (mTLE) patients as surgical therapeutic approach. Direct insight into this epileptogenic area, by two powerful techniques,
optical emission and mass spectrometry, has led us to investigation of copper turnover. Namely, among the examined metals,
we found the deficiency of copper in sclerotic hippocampus on two levels: (i) in whole structure (ii) and locally in the areas of
neuronal loss, with significant correlation between copper concentration and neuron density. Furthermore, analysis of copper
metalloproteins showed: (i) significant increase or decrease in levels of protein that is participating in copper transport into
the cell (CTR1) depending on the degree of hippocampal neuronal loss; (ii) and lower activity of an enzyme in which copper
is part of the active site, cytochrome c oxidase, in sclerotic hippocampi of patients compared to control tissue. In our further
investigations it remained to be determined whether changes in copper concentrations and copper metalloproteins are causal
to pathology of mTLE or they represent epiphenomenon.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "The importance of copper in pathology of mesial temporal lobe epilepsy",
pages = "46",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5882"
}

Savić, D., Opačić, M., Brkljačić, J., Ristić, A., Sokić, D., Baščarević, V., Raičević, S., Savić, S., Zorović, M., Živin, M., Šelih, V. S., Spasić, S.,& Spasojević, I.. (2019). The importance of copper in pathology of mesial temporal lobe epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 46.
https://hdl.handle.net/21.15107/rcub_ibiss_5882

Savić D, Opačić M, Brkljačić J, Ristić A, Sokić D, Baščarević V, Raičević S, Savić S, Zorović M, Živin M, Šelih VS, Spasić S, Spasojević I. The importance of copper in pathology of mesial temporal lobe epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:46.
https://hdl.handle.net/21.15107/rcub_ibiss_5882 .

Savić, Danijela, Opačić, Miloš, Brkljačić, Jelena, Ristić, Aleksandar, Sokić, Dragoslav, Baščarević, Vladimir, Raičević, Savo, Savić, Slobodan, Zorović, Maja, Živin, Marko, Šelih, Vid Simon, Spasić, Snežana, Spasojević, Ivan, "The importance of copper in pathology of mesial temporal lobe epilepsy" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):46,
https://hdl.handle.net/21.15107/rcub_ibiss_5882 .

TY  - CONF
AU  - Opačić, Miloš
AU  - Zorović, Maja
AU  - Savić, Danijela
AU  - Živin, Marko
AU  - Raičević, Savo
AU  - Baščarević, Vladimir
AU  - Ristić, Aleksandar
AU  - Sokić, Dragoslav
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5986
AB  - Aims: A drop in copper level and the loss of energy homeostasis are both portrayed in mesial temporal lobe epilepsy (mTLE) with hippocampal sclerosis (HS) patients. Cytochrome c oxidase (COX) represents a crossroad of energy and copper metabolism; it is a key component of mitochondrial machinery and contains two copper centers. Our aim here was to examine the link between COX activity and the copper transporting system in HS. COX activity and the levels of mRNA of selected chaperones - COX11, COX17, Sco1 and Sco2 were determined in 13 anatomically distinct hippocampal regions. Methods: Study was performed on seven hippocampal samples, four of which had been acquired during the course of amygdalohippocampectomy treatment of medically intractable epilepsy and three control postmortem samples. Adjacent slices were used for Nissl staining, COX activity assay and mRNA in situ hybridization with autoradiography. Densitometry was performed using ImageJ. Results: Overall COX activity was decreased in HS compared to controls (P = 0.0003). However, 5 regions showed significantly lower COX activity in HS and 8 did not. Subiculum showed slightly higher activity in HS. The levels of mRNA levels were lowered in HS in 6 regions for COX11, 10 regions for COX17, two regions for Sco1 and 11 regions for Sco2. Conclusions: Our findings suggest the loss of energy homeostasis in HS may be related to pathological changes in specific components of copper delivery to COX, and that the impact may vary between different hippocampal regions.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients
SP  - 296
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5986
ER  - 

@conference{
author = "Opačić, Miloš and Zorović, Maja and Savić, Danijela and Živin, Marko and Raičević, Savo and Baščarević, Vladimir and Ristić, Aleksandar and Sokić, Dragoslav and Spasojević, Ivan",
year = "2019",
abstract = "Aims: A drop in copper level and the loss of energy homeostasis are both portrayed in mesial temporal lobe epilepsy (mTLE) with hippocampal sclerosis (HS) patients. Cytochrome c oxidase (COX) represents a crossroad of energy and copper metabolism; it is a key component of mitochondrial machinery and contains two copper centers. Our aim here was to examine the link between COX activity and the copper transporting system in HS. COX activity and the levels of mRNA of selected chaperones - COX11, COX17, Sco1 and Sco2 were determined in 13 anatomically distinct hippocampal regions. Methods: Study was performed on seven hippocampal samples, four of which had been acquired during the course of amygdalohippocampectomy treatment of medically intractable epilepsy and three control postmortem samples. Adjacent slices were used for Nissl staining, COX activity assay and mRNA in situ hybridization with autoradiography. Densitometry was performed using ImageJ. Results: Overall COX activity was decreased in HS compared to controls (P = 0.0003). However, 5 regions showed significantly lower COX activity in HS and 8 did not. Subiculum showed slightly higher activity in HS. The levels of mRNA levels were lowered in HS in 6 regions for COX11, 10 regions for COX17, two regions for Sco1 and 11 regions for Sco2. Conclusions: Our findings suggest the loss of energy homeostasis in HS may be related to pathological changes in specific components of copper delivery to COX, and that the impact may vary between different hippocampal regions.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients",
pages = "296",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5986"
}

Opačić, M., Zorović, M., Savić, D., Živin, M., Raičević, S., Baščarević, V., Ristić, A., Sokić, D.,& Spasojević, I.. (2019). Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 296.
https://hdl.handle.net/21.15107/rcub_ibiss_5986

Opačić M, Zorović M, Savić D, Živin M, Raičević S, Baščarević V, Ristić A, Sokić D, Spasojević I. Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:296.
https://hdl.handle.net/21.15107/rcub_ibiss_5986 .

Opačić, Miloš, Zorović, Maja, Savić, Danijela, Živin, Marko, Raičević, Savo, Baščarević, Vladimir, Ristić, Aleksandar, Sokić, Dragoslav, Spasojević, Ivan, "Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):296,
https://hdl.handle.net/21.15107/rcub_ibiss_5986 .

TY  - CONF
AU  - Nikolić, Ljiljana
AU  - Korać, Jelena
AU  - Bijelić, Dunja
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5510
AB  - Iron, an essential element for living organisms, participates in a wide range of metabolic
processes. It appears predominantly firmly bound to proteins, but can also be loosely bound to
low-affinity ligands, referred as labile iron pool (LIP). The composition and amount of LIP can
vary considerably under different physiological conditions, playing a beneficial role in iron
economy and homeostasis or contributing to the generation of reactive oxygen species. It is
still not known if bioactivity of low-affinity ligands can be modulated by iron binding.
Catecholamine neurotransmitters including norepinephrine (NE) can chelate iron. In the close
vicinity of synaptic cleft, astrocytes are direct target of norepinephrine. Here we show on
cultured rat cortical astrocytes that iron bound to NE completely blocks neurotransmitter
activity of NE. However, how astrocyte activity changes when norepinephrine binds iron
remains unknown.
We show, using spectrophotometry that NE and Fe3+ form complex in the 1:1 stoichiometry,
at pH 7.4. Iron effect on astrocyte response to NE was examined by the whole-cell patch-clamp
technique. NE alone evokes changes in the membrane currents of astrocytes, but such effects
were not observed for the NE- Fe3+ complex.
Our results demonstrating that iron in the complex with norepinephrine inhibits alpha-adrenergic
receptors and modulates astrocyte activity, imply a novel neuromodulatory role for LIP.
PB  - COST Action CA15133
C3  - Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland
T1  - Iron modulates norepinephrine effect on astrocytes
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5510
ER  - 

@conference{
author = "Nikolić, Ljiljana and Korać, Jelena and Bijelić, Dunja and Spasojević, Ivan and Bogdanović Pristov, Jelena",
year = "2019",
abstract = "Iron, an essential element for living organisms, participates in a wide range of metabolic
processes. It appears predominantly firmly bound to proteins, but can also be loosely bound to
low-affinity ligands, referred as labile iron pool (LIP). The composition and amount of LIP can
vary considerably under different physiological conditions, playing a beneficial role in iron
economy and homeostasis or contributing to the generation of reactive oxygen species. It is
still not known if bioactivity of low-affinity ligands can be modulated by iron binding.
Catecholamine neurotransmitters including norepinephrine (NE) can chelate iron. In the close
vicinity of synaptic cleft, astrocytes are direct target of norepinephrine. Here we show on
cultured rat cortical astrocytes that iron bound to NE completely blocks neurotransmitter
activity of NE. However, how astrocyte activity changes when norepinephrine binds iron
remains unknown.
We show, using spectrophotometry that NE and Fe3+ form complex in the 1:1 stoichiometry,
at pH 7.4. Iron effect on astrocyte response to NE was examined by the whole-cell patch-clamp
technique. NE alone evokes changes in the membrane currents of astrocytes, but such effects
were not observed for the NE- Fe3+ complex.
Our results demonstrating that iron in the complex with norepinephrine inhibits alpha-adrenergic
receptors and modulates astrocyte activity, imply a novel neuromodulatory role for LIP.",
publisher = "COST Action CA15133",
journal = "Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland",
title = "Iron modulates norepinephrine effect on astrocytes",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5510"
}

Nikolić, L., Korać, J., Bijelić, D., Spasojević, I.,& Bogdanović Pristov, J.. (2019). Iron modulates norepinephrine effect on astrocytes. in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland
COST Action CA15133..
https://hdl.handle.net/21.15107/rcub_ibiss_5510

Nikolić L, Korać J, Bijelić D, Spasojević I, Bogdanović Pristov J. Iron modulates norepinephrine effect on astrocytes. in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland. 2019;.
https://hdl.handle.net/21.15107/rcub_ibiss_5510 .

Nikolić, Ljiljana, Korać, Jelena, Bijelić, Dunja, Spasojević, Ivan, Bogdanović Pristov, Jelena, "Iron modulates norepinephrine effect on astrocytes" in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland (2019),
https://hdl.handle.net/21.15107/rcub_ibiss_5510 .

TY  - CONF
AU  - Korać, Jelena
AU  - Nikolić, Ljiljana
AU  - Bijelić, Dunja
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5501
AB  - Aims: Astrocyte position between synapses and blood vessels allows them to ful l crucial functions such as regulation of
synaptic activity and potassium bu ering. Well positioned in the close vicinity of synaptic cleft astrocytes are considered to be
a direct target of norepinephrine (NE). Synaptic activity and neurotransmitter actions can be in uenced by extracellular iron.
Here we investigated whether iron interacts with NE and if this interaction can modulate astrocyte response to NE.
Methods: To investigate the interaction between iron and norepinephrine we used spectrophotometry approach. Iron e ect
on astrocyte response to NE was examined by the whole-cell patch-clamp technique. Membrane currents were recorded from
cultured cortical astrocytes prepared from WT rats.
Results: Using spectrophotometry we observed that iron interacts with NE which leads to the formation of a stable complex
in the 1:1 stoichiometry. We also found that iron bound to NE completely blocks NE-induced increase of large-conductance
calcium sensitive potassium current in astrocytes.
Conclusions: Astrocyte response to NE is modi ed when this neurotransmitter forms a complex with iron. This implies that
NE binding to astrocytic noradrenergic receptors may be prevented by iron. Our  ndings point toward compromised astrocyte
functions related to the potassium bu ering when NE action is modi ed by iron.
PB  - Belgrade : Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Iron modulates norepinephrine effect on astrocytes
SP  - 370
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5501
ER  - 

@conference{
author = "Korać, Jelena and Nikolić, Ljiljana and Bijelić, Dunja and Spasojević, Ivan and Bogdanović Pristov, Jelena",
year = "2019",
abstract = "Aims: Astrocyte position between synapses and blood vessels allows them to ful l crucial functions such as regulation of
synaptic activity and potassium bu ering. Well positioned in the close vicinity of synaptic cleft astrocytes are considered to be
a direct target of norepinephrine (NE). Synaptic activity and neurotransmitter actions can be in uenced by extracellular iron.
Here we investigated whether iron interacts with NE and if this interaction can modulate astrocyte response to NE.
Methods: To investigate the interaction between iron and norepinephrine we used spectrophotometry approach. Iron e ect
on astrocyte response to NE was examined by the whole-cell patch-clamp technique. Membrane currents were recorded from
cultured cortical astrocytes prepared from WT rats.
Results: Using spectrophotometry we observed that iron interacts with NE which leads to the formation of a stable complex
in the 1:1 stoichiometry. We also found that iron bound to NE completely blocks NE-induced increase of large-conductance
calcium sensitive potassium current in astrocytes.
Conclusions: Astrocyte response to NE is modi ed when this neurotransmitter forms a complex with iron. This implies that
NE binding to astrocytic noradrenergic receptors may be prevented by iron. Our  ndings point toward compromised astrocyte
functions related to the potassium bu ering when NE action is modi ed by iron.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Iron modulates norepinephrine effect on astrocytes",
pages = "370",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5501"
}

Korać, J., Nikolić, L., Bijelić, D., Spasojević, I.,& Bogdanović Pristov, J.. (2019). Iron modulates norepinephrine effect on astrocytes. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade : Serbian Neuroscience Society., 370.
https://hdl.handle.net/21.15107/rcub_ibiss_5501

Korać J, Nikolić L, Bijelić D, Spasojević I, Bogdanović Pristov J. Iron modulates norepinephrine effect on astrocytes. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:370.
https://hdl.handle.net/21.15107/rcub_ibiss_5501 .

Korać, Jelena, Nikolić, Ljiljana, Bijelić, Dunja, Spasojević, Ivan, Bogdanović Pristov, Jelena, "Iron modulates norepinephrine effect on astrocytes" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):370,
https://hdl.handle.net/21.15107/rcub_ibiss_5501 .

TY  - CONF
AU  - Savić, Danijela
AU  - Opačić, Miloš
AU  - Ristić, Aleksandar
AU  - Sokić, Dragoslav
AU  - Baščarević, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Živin, Marko
AU  - Šelih, Vid Simon
AU  - Spasić, Snežana
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5887
AB  - The accumulating evidence on the relation between the disturbed metal homeostasis
and epilepsy urges the need for data regarding the total metal concentrations, as well
as metal distribution in the brain itself, in order to indicate where to direct the
potential therapy, to metal supplementation or chelation. This paper summarizes our
results on the measurements of some important essential metals in hippocampi of
patients with mesial temporal lobe epilepsy (mTLE) who underwent
amigdalohippocampectomy. The key findings point out that levels of copper and
manganese are deficient in hippocampi of mTLE patients, and that their
concentrations correlated positively with neuronal loss in affected regions of sclerotic
hippocampus. In addition, the Cu concentration was decreased in the areas of total
neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor
deficient compared to control. Our results contribute to deeper insight of metals
biology in the epilepsy and may represent the initial point of new and non-invasive
therapy of drug resistant epilepsy.
PB  - Belgrade: Faculty of Chemistry: Serbian Biochemical Society
C3  - Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia
T1  - Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy
SP  - 105
EP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5887
ER  - 

@conference{
author = "Savić, Danijela and Opačić, Miloš and Ristić, Aleksandar and Sokić, Dragoslav and Baščarević, Vladimir and Raičević, Savo and Savić, Slobodan and Živin, Marko and Šelih, Vid Simon and Spasić, Snežana and Spasojević, Ivan",
year = "2018",
abstract = "The accumulating evidence on the relation between the disturbed metal homeostasis
and epilepsy urges the need for data regarding the total metal concentrations, as well
as metal distribution in the brain itself, in order to indicate where to direct the
potential therapy, to metal supplementation or chelation. This paper summarizes our
results on the measurements of some important essential metals in hippocampi of
patients with mesial temporal lobe epilepsy (mTLE) who underwent
amigdalohippocampectomy. The key findings point out that levels of copper and
manganese are deficient in hippocampi of mTLE patients, and that their
concentrations correlated positively with neuronal loss in affected regions of sclerotic
hippocampus. In addition, the Cu concentration was decreased in the areas of total
neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor
deficient compared to control. Our results contribute to deeper insight of metals
biology in the epilepsy and may represent the initial point of new and non-invasive
therapy of drug resistant epilepsy.",
publisher = "Belgrade: Faculty of Chemistry: Serbian Biochemical Society",
journal = "Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia",
title = "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy",
pages = "105-112",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5887"
}

Savić, D., Opačić, M., Ristić, A., Sokić, D., Baščarević, V., Raičević, S., Savić, S., Živin, M., Šelih, V. S., Spasić, S.,& Spasojević, I.. (2018). Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia
Belgrade: Faculty of Chemistry: Serbian Biochemical Society., 105-112.
https://hdl.handle.net/21.15107/rcub_ibiss_5887

Savić D, Opačić M, Ristić A, Sokić D, Baščarević V, Raičević S, Savić S, Živin M, Šelih VS, Spasić S, Spasojević I. Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia. 2018;:105-112.
https://hdl.handle.net/21.15107/rcub_ibiss_5887 .

Savić, Danijela, Opačić, Miloš, Ristić, Aleksandar, Sokić, Dragoslav, Baščarević, Vladimir, Raičević, Savo, Savić, Slobodan, Živin, Marko, Šelih, Vid Simon, Spasić, Snežana, Spasojević, Ivan, "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy" in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia (2018):105-112,
https://hdl.handle.net/21.15107/rcub_ibiss_5887 .

TY  - CONF
AU  - Korać, Jelena
AU  - Stanković, Dalibor
AU  - Bogdanović Pristov, Jelena
AU  - Nikolić, Ljiljana
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5517
AB  - Epinephrine ((R)-4-(1-hydroxy-2-(methylamino)ethyl)-benzene-1,2-diol (Epi) is
catecholamine that is released by the sympathetic nervous system and adrenal medulla. It
is a physiologically important molecule that acts as a hormone, neurotransmitter, and
medication with a broad range of effects 1-3 . Coordinate and redox interaction of Epi with
iron affects the interactions with other molecules and its biological effects 4
. In this study,
we reported details of redox interactions of Epi with Fe 2+ at pH 7.4, which correspond to
the pH value of human plasma Epi and Fe 2+ form a complex that acts as a strong reducing
agent. Cyclic voltammetry showed that the positions of E pa and E pc potentials were at
approximately -480 and -1100 mV. This implies that Epi and Fe 2+ build a complex with
unique redox properties. E1/2 was significantly lower compared to E0' for O 2 /O 2•- (-350
mV). It is important to point out this because superoxide radical anion is produced via
spontaneous Fe 2+ reaction with O 2. In other words, Epi-Fe 2+ complex should be capable of
reducing transition metals in (patho)physiologicaly relevant complexes that are not
susceptible to reduction by O 2. Our results confirmed that Epi-Fe
2+ is capable of reducing
the S-S group of glutathione disulfide. On the other hand, Epi acted in a catalyst-like
fashion to promote Fe 2+ oxidation by molecular oxygen, and to a facilitated formation of
the Epi–Fe 3+ complexes, at physiological pH. In addition, we examined the effects of
epinepfrine and Epi/Fe3+ system on glioma cells. Epinephrine alone evokes changes in the
membrane currents of glioma cells, but such effects were not observed for the complex
with Fe 3+
. This implies that Epi-Fe 3+ might modulate neural activity of Epi in CNS.
PB  - Belgrade : Faculty of Chemistry : Serbian Biochemical Society
C3  - Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia
T1  - Redox interactions of epinephrine with iron at physiological pH
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5517
ER  - 

@conference{
author = "Korać, Jelena and Stanković, Dalibor and Bogdanović Pristov, Jelena and Nikolić, Ljiljana and Spasojević, Ivan",
year = "2018",
abstract = "Epinephrine ((R)-4-(1-hydroxy-2-(methylamino)ethyl)-benzene-1,2-diol (Epi) is
catecholamine that is released by the sympathetic nervous system and adrenal medulla. It
is a physiologically important molecule that acts as a hormone, neurotransmitter, and
medication with a broad range of effects 1-3 . Coordinate and redox interaction of Epi with
iron affects the interactions with other molecules and its biological effects 4
. In this study,
we reported details of redox interactions of Epi with Fe 2+ at pH 7.4, which correspond to
the pH value of human plasma Epi and Fe 2+ form a complex that acts as a strong reducing
agent. Cyclic voltammetry showed that the positions of E pa and E pc potentials were at
approximately -480 and -1100 mV. This implies that Epi and Fe 2+ build a complex with
unique redox properties. E1/2 was significantly lower compared to E0' for O 2 /O 2•- (-350
mV). It is important to point out this because superoxide radical anion is produced via
spontaneous Fe 2+ reaction with O 2. In other words, Epi-Fe 2+ complex should be capable of
reducing transition metals in (patho)physiologicaly relevant complexes that are not
susceptible to reduction by O 2. Our results confirmed that Epi-Fe
2+ is capable of reducing
the S-S group of glutathione disulfide. On the other hand, Epi acted in a catalyst-like
fashion to promote Fe 2+ oxidation by molecular oxygen, and to a facilitated formation of
the Epi–Fe 3+ complexes, at physiological pH. In addition, we examined the effects of
epinepfrine and Epi/Fe3+ system on glioma cells. Epinephrine alone evokes changes in the
membrane currents of glioma cells, but such effects were not observed for the complex
with Fe 3+
. This implies that Epi-Fe 3+ might modulate neural activity of Epi in CNS.",
publisher = "Belgrade : Faculty of Chemistry : Serbian Biochemical Society",
journal = "Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia",
title = "Redox interactions of epinephrine with iron at physiological pH",
pages = "141",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5517"
}

Korać, J., Stanković, D., Bogdanović Pristov, J., Nikolić, L.,& Spasojević, I.. (2018). Redox interactions of epinephrine with iron at physiological pH. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia
Belgrade : Faculty of Chemistry : Serbian Biochemical Society., 141.
https://hdl.handle.net/21.15107/rcub_ibiss_5517

Korać J, Stanković D, Bogdanović Pristov J, Nikolić L, Spasojević I. Redox interactions of epinephrine with iron at physiological pH. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia. 2018;:141.
https://hdl.handle.net/21.15107/rcub_ibiss_5517 .

Korać, Jelena, Stanković, Dalibor, Bogdanović Pristov, Jelena, Nikolić, Ljiljana, Spasojević, Ivan, "Redox interactions of epinephrine with iron at physiological pH" in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia (2018):141,
https://hdl.handle.net/21.15107/rcub_ibiss_5517 .

TY  - JOUR
AU  - Opačić, Miloš
AU  - Stević, Zorica
AU  - Baščarević, Vladimir
AU  - Živić, Miroslav
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://www.liebertpub.com/doi/10.1089/ars.2017.7433
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3065
AB  - The monitoring of progression in amyotrophic lateral sclerosis (ALS) relies on clinical outcome measures that take months to interpret, such as revised ALS functional rating scale (ALSFRS-R) score, with no approved biomarkers. A number of clinical studies have documented the involvement of oxidative stress in ALS pathology. Pertinent to this, we propose to evaluate oxidation-reduction potential (ORP) of cerebrospinal fluid (CSF) as a potential indicator of ALS progression. The case-control study included 24 patients with neurological non-neurodegenerative disorders (controls) and 82 ALS patients with different degrees of disease (ALSFRS-R score: 21-47). ORP was significantly higher in ALS patients than controls. It was not dependent on age or gender. A strong negative correlation was found between ORP and ALSFRS-R score for all patients and patients with spinal onset. In other words, ORP increased with ALS progression. No correlation was found for the subset of patients with bulbar onset, most likely because of the physical distance between neurodegenerative loci and the site of CSF collection. These results lead to the hypothesis that ORP of CSF has a potential as monitoring biomarker in ALS, particularly in the cohort of patients with spinal onset. Antioxid. Redox Signal. 28, 1570-1575.
T2  - Antioxidants and Redox Signaling
T1  - Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?
IS  - 17
VL  - 28
DO  - 10.1089/ars.2017.7433
SP  - 1570
EP  - 1575
ER  - 

@article{
author = "Opačić, Miloš and Stević, Zorica and Baščarević, Vladimir and Živić, Miroslav and Spasić, Mihajlo and Spasojević, Ivan",
year = "2018",
abstract = "The monitoring of progression in amyotrophic lateral sclerosis (ALS) relies on clinical outcome measures that take months to interpret, such as revised ALS functional rating scale (ALSFRS-R) score, with no approved biomarkers. A number of clinical studies have documented the involvement of oxidative stress in ALS pathology. Pertinent to this, we propose to evaluate oxidation-reduction potential (ORP) of cerebrospinal fluid (CSF) as a potential indicator of ALS progression. The case-control study included 24 patients with neurological non-neurodegenerative disorders (controls) and 82 ALS patients with different degrees of disease (ALSFRS-R score: 21-47). ORP was significantly higher in ALS patients than controls. It was not dependent on age or gender. A strong negative correlation was found between ORP and ALSFRS-R score for all patients and patients with spinal onset. In other words, ORP increased with ALS progression. No correlation was found for the subset of patients with bulbar onset, most likely because of the physical distance between neurodegenerative loci and the site of CSF collection. These results lead to the hypothesis that ORP of CSF has a potential as monitoring biomarker in ALS, particularly in the cohort of patients with spinal onset. Antioxid. Redox Signal. 28, 1570-1575.",
journal = "Antioxidants and Redox Signaling",
title = "Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?",
number = "17",
volume = "28",
doi = "10.1089/ars.2017.7433",
pages = "1570-1575"
}

Opačić, M., Stević, Z., Baščarević, V., Živić, M., Spasić, M.,& Spasojević, I.. (2018). Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?. in Antioxidants and Redox Signaling, 28(17), 1570-1575.
https://doi.org/10.1089/ars.2017.7433

Opačić M, Stević Z, Baščarević V, Živić M, Spasić M, Spasojević I. Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?. in Antioxidants and Redox Signaling. 2018;28(17):1570-1575.
doi:10.1089/ars.2017.7433 .

Opačić, Miloš, Stević, Zorica, Baščarević, Vladimir, Živić, Miroslav, Spasić, Mihajlo, Spasojević, Ivan, "Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?" in Antioxidants and Redox Signaling, 28, no. 17 (2018):1570-1575,
https://doi.org/10.1089/ars.2017.7433 . .

TY  - JOUR
AU  - Martinov, Jelena
AU  - Krstić, Miodrag
AU  - Spasić, Snežana
AU  - Miletić, Srdjan
AU  - Stefanović-Kojić, Jovana
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0963996917304787
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2843
AB  - Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Fourier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin-derived oligosaccharides on hydroxyl radical (HO)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus aureus in the presence of dietary-relevant HO-generating system (iron+ascorbate). The oligosaccharides react with HO radical to produce carbon dioxide radical anion (CO 2 - ). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO 2 - , which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO.
T2  - Food Research International
T1  - Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus
DO  - 10.1016/j.foodres.2017.08.040
ER  - 

@article{
author = "Martinov, Jelena and Krstić, Miodrag and Spasić, Snežana and Miletić, Srdjan and Stefanović-Kojić, Jovana and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Spasojević, Ivan and Spasić, Mihajlo",
year = "2017",
abstract = "Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Fourier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin-derived oligosaccharides on hydroxyl radical (HO)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus aureus in the presence of dietary-relevant HO-generating system (iron+ascorbate). The oligosaccharides react with HO radical to produce carbon dioxide radical anion (CO 2 - ). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO 2 - , which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO.",
journal = "Food Research International",
title = "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus",
doi = "10.1016/j.foodres.2017.08.040"
}

Martinov, J., Krstić, M., Spasić, S., Miletić, S., Stefanović-Kojić, J., Nikolić-Kokić, A., Blagojević, D., Spasojević, I.,& Spasić, M.. (2017). Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International.
https://doi.org/10.1016/j.foodres.2017.08.040

Martinov J, Krstić M, Spasić S, Miletić S, Stefanović-Kojić J, Nikolić-Kokić A, Blagojević D, Spasojević I, Spasić M. Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International. 2017;.
doi:10.1016/j.foodres.2017.08.040 .

Martinov, Jelena, Krstić, Miodrag, Spasić, Snežana, Miletić, Srdjan, Stefanović-Kojić, Jovana, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Spasojević, Ivan, Spasić, Mihajlo, "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus" in Food Research International (2017),
https://doi.org/10.1016/j.foodres.2017.08.040 . .

TY  - JOUR
AU  - Opačić, Miloš
AU  - Ristić, Aleksandar J
AU  - Savić, Danijela
AU  - Šelih, Vid Simon
AU  - Živin, Marko
AU  - Sokić, Dragoslav
AU  - Raičević, Savo
AU  - Baščarević, Vladimir
AU  - Spasojević, Ivan
PY  - 2017
UR  - http://xlink.rsc.org/?DOI=C6MT00293E
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28124054
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2583
AB  - The loss of metal homeostasis has been implicated in the pathophysiology of mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). Here we applied laser ablation inductively coupled plasma mass spectrometry imaging to establish the spatial distribution of Zn, Fe, Cu and Mn in coronal sections of hippocampi of four patients with drug-resistant mTLE-HS who underwent amygdalohippocampectomy. Detailed maps of the metal concentrations in the different morphological areas/layers were built and analyzed. The highest level of Zn (>20 μg g(-1)) was found in mossy fiber-rich regions - cornu ammonis field 4 (CA4), gyrus dentatus, and CA3. The distribution of Fe appears to reflect the routes of the main intrahippocampal blood vessels. The highest concentrations of Cu (>10 μg g(-1)) and Mn (>15 μg g(-1)) were observed in regions/layers with neuron somata - subiculum, CA4, gyrus dentatus, and stratum pyramidale (SPy) in CA1 and CA2. Alveus and other regions with axons and dendrites generally showed lower levels of Zn, Cu, and Mn. The Cu concentration was decreased in the areas of total neuronal loss in SPy in CA1 (9.73 ± 0.91 μg g(-1)), compared to the subiculum (13.32 ± 1.29 μg g(-1); p = 0.043). The Cu and Mn concentrations correlated positively with neuron density in the SPy in CA1 (R = 0.629, p < 0.001; and R = 0.391, p = 0.004). These results provide a deeper insight into hippocampal metabolism of metals, and pave the road for identifying the components of the mechanism of epileptogenesis among Cu and Mn transporters and metalloproteins.
T2  - Metallomics : integrated biometal science
T1  - Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy.
IS  - 2
VL  - 9
DO  - 10.1039/c6mt00293e
SP  - 141
EP  - 148
ER  - 

@article{
author = "Opačić, Miloš and Ristić, Aleksandar J and Savić, Danijela and Šelih, Vid Simon and Živin, Marko and Sokić, Dragoslav and Raičević, Savo and Baščarević, Vladimir and Spasojević, Ivan",
year = "2017",
abstract = "The loss of metal homeostasis has been implicated in the pathophysiology of mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). Here we applied laser ablation inductively coupled plasma mass spectrometry imaging to establish the spatial distribution of Zn, Fe, Cu and Mn in coronal sections of hippocampi of four patients with drug-resistant mTLE-HS who underwent amygdalohippocampectomy. Detailed maps of the metal concentrations in the different morphological areas/layers were built and analyzed. The highest level of Zn (>20 μg g(-1)) was found in mossy fiber-rich regions - cornu ammonis field 4 (CA4), gyrus dentatus, and CA3. The distribution of Fe appears to reflect the routes of the main intrahippocampal blood vessels. The highest concentrations of Cu (>10 μg g(-1)) and Mn (>15 μg g(-1)) were observed in regions/layers with neuron somata - subiculum, CA4, gyrus dentatus, and stratum pyramidale (SPy) in CA1 and CA2. Alveus and other regions with axons and dendrites generally showed lower levels of Zn, Cu, and Mn. The Cu concentration was decreased in the areas of total neuronal loss in SPy in CA1 (9.73 ± 0.91 μg g(-1)), compared to the subiculum (13.32 ± 1.29 μg g(-1); p = 0.043). The Cu and Mn concentrations correlated positively with neuron density in the SPy in CA1 (R = 0.629, p < 0.001; and R = 0.391, p = 0.004). These results provide a deeper insight into hippocampal metabolism of metals, and pave the road for identifying the components of the mechanism of epileptogenesis among Cu and Mn transporters and metalloproteins.",
journal = "Metallomics : integrated biometal science",
title = "Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy.",
number = "2",
volume = "9",
doi = "10.1039/c6mt00293e",
pages = "141-148"
}

Opačić, M., Ristić, A. J., Savić, D., Šelih, V. S., Živin, M., Sokić, D., Raičević, S., Baščarević, V.,& Spasojević, I.. (2017). Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy.. in Metallomics : integrated biometal science, 9(2), 141-148.
https://doi.org/10.1039/c6mt00293e

Opačić M, Ristić AJ, Savić D, Šelih VS, Živin M, Sokić D, Raičević S, Baščarević V, Spasojević I. Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy.. in Metallomics : integrated biometal science. 2017;9(2):141-148.
doi:10.1039/c6mt00293e .

Opačić, Miloš, Ristić, Aleksandar J, Savić, Danijela, Šelih, Vid Simon, Živin, Marko, Sokić, Dragoslav, Raičević, Savo, Baščarević, Vladimir, Spasojević, Ivan, "Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy." in Metallomics : integrated biometal science, 9, no. 2 (2017):141-148,
https://doi.org/10.1039/c6mt00293e . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin-Dušić, Zorana
AU  - Spasojević, Ivan
AU  - Blagojević, Duško
AU  - Stević, Zorica
AU  - Anđus, Pavle
AU  - Spasić, Mihajlo
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6080
AB  - In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - The Effects of Wild-Type and Mutant SOD1 on Smooth Muscle Contraction
IS  - 1
VL  - 67
DO  - 10.2298/ABS141006023N
SP  - 187
EP  - 192
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin-Dušić, Zorana and Spasojević, Ivan and Blagojević, Duško and Stević, Zorica and Anđus, Pavle and Spasić, Mihajlo",
year = "2015",
abstract = "In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "The Effects of Wild-Type and Mutant SOD1 on Smooth Muscle Contraction",
number = "1",
volume = "67",
doi = "10.2298/ABS141006023N",
pages = "187-192"
}

Nikolić-Kokić, A., Oreščanin-Dušić, Z., Spasojević, I., Blagojević, D., Stević, Z., Anđus, P.,& Spasić, M.. (2015). The Effects of Wild-Type and Mutant SOD1 on Smooth Muscle Contraction. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 67(1), 187-192.
https://doi.org/10.2298/ABS141006023N

Nikolić-Kokić A, Oreščanin-Dušić Z, Spasojević I, Blagojević D, Stević Z, Anđus P, Spasić M. The Effects of Wild-Type and Mutant SOD1 on Smooth Muscle Contraction. in Archives of Biological Sciences. 2015;67(1):187-192.
doi:10.2298/ABS141006023N .

Nikolić-Kokić, Aleksandra, Oreščanin-Dušić, Zorana, Spasojević, Ivan, Blagojević, Duško, Stević, Zorica, Anđus, Pavle, Spasić, Mihajlo, "The Effects of Wild-Type and Mutant SOD1 on Smooth Muscle Contraction" in Archives of Biological Sciences, 67, no. 1 (2015):187-192,
https://doi.org/10.2298/ABS141006023N . .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Spasojević, Ivan
AU  - Konić-Ristić, Aleksandra
AU  - Milenković, Dragan
AU  - Živanović, Jasmina
AU  - Jarić, Ivana
AU  - Milošević, Verica
PY  - 2014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6241
AB  - Superficial membrane fluidity is related to erythrocyte deformation behavior and affects the hemodynamics, especially in hypertension and atherosclerosis. Grapefruit flavanone naringenin, which is used as alternative therapeutic of cardiovascular conditions, can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of two nutritional doses of naringenin (lower - 0.1 µg/ml and higher - 1 µg/ml) on superficial membrane fluidity in human erythrocytes, which reflects their deformability. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 7-DS), the spectra of which are dependent on membrane fluidity. The results showed a significant (p=0.029) increase of membrane fluidity near the hydrophilic surface in erythrocytes treated with higher nutritional dose of naringenin, while the lower nutritional dose of naringenin was ineffective (p>0.05) in that membrane region. In the hydrophobic layer just below the erythrocyte membrane phospholipid heads both lower and higher dose of naringenin significantly (p=0.036 and p=0.028, respectively) increased membrane fluidity. These data document the positive and dose dependent effect of naringenin on superficial membrane fluidity in human erythrocytes, recommending its use in the cardiovascular conditions characterized by disturbed hemodynamics.
PB  - Belgrade: Serbian Physiological Society
C3  - Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia
T1  - The positive effect of naringenin on superficial membrane fluidity in human erythrocytes
SP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6241
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Spasojević, Ivan and Konić-Ristić, Aleksandra and Milenković, Dragan and Živanović, Jasmina and Jarić, Ivana and Milošević, Verica",
year = "2014",
abstract = "Superficial membrane fluidity is related to erythrocyte deformation behavior and affects the hemodynamics, especially in hypertension and atherosclerosis. Grapefruit flavanone naringenin, which is used as alternative therapeutic of cardiovascular conditions, can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of two nutritional doses of naringenin (lower - 0.1 µg/ml and higher - 1 µg/ml) on superficial membrane fluidity in human erythrocytes, which reflects their deformability. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 7-DS), the spectra of which are dependent on membrane fluidity. The results showed a significant (p=0.029) increase of membrane fluidity near the hydrophilic surface in erythrocytes treated with higher nutritional dose of naringenin, while the lower nutritional dose of naringenin was ineffective (p>0.05) in that membrane region. In the hydrophobic layer just below the erythrocyte membrane phospholipid heads both lower and higher dose of naringenin significantly (p=0.036 and p=0.028, respectively) increased membrane fluidity. These data document the positive and dose dependent effect of naringenin on superficial membrane fluidity in human erythrocytes, recommending its use in the cardiovascular conditions characterized by disturbed hemodynamics.",
publisher = "Belgrade: Serbian Physiological Society",
journal = "Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia",
title = "The positive effect of naringenin on superficial membrane fluidity in human erythrocytes",
pages = "63",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6241"
}

Ajdžanović, V., Spasojević, I., Konić-Ristić, A., Milenković, D., Živanović, J., Jarić, I.,& Milošević, V.. (2014). The positive effect of naringenin on superficial membrane fluidity in human erythrocytes. in Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia
Belgrade: Serbian Physiological Society., 63.
https://hdl.handle.net/21.15107/rcub_ibiss_6241

Ajdžanović V, Spasojević I, Konić-Ristić A, Milenković D, Živanović J, Jarić I, Milošević V. The positive effect of naringenin on superficial membrane fluidity in human erythrocytes. in Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia. 2014;:63.
https://hdl.handle.net/21.15107/rcub_ibiss_6241 .

Ajdžanović, Vladimir, Spasojević, Ivan, Konić-Ristić, Aleksandra, Milenković, Dragan, Živanović, Jasmina, Jarić, Ivana, Milošević, Verica, "The positive effect of naringenin on superficial membrane fluidity in human erythrocytes" in Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia (2014):63,
https://hdl.handle.net/21.15107/rcub_ibiss_6241 .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Oreščanin Dušić, Zorana
AU  - Slavić, Marija
AU  - Spasojević, Ivan
AU  - Stević, Zorica
AU  - Spasić, Mihajlo
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/504
AB  - Background: Mutated copper, zinc-containing superoxide dismutase (SOD1) may self-aggregate, an event that could also be an initial cause of motor neuron malfunction leading to disease onset. The effects of human mutated SOD1 protein from the blood of familial amyotrophic lateral sclerosis (FALS) patients bearing Leu144Phe (L144F) mutation were compared to wild-type (WT) human SOD1 derived from healthy examinees, for enzymatic activity and the effects on isometric contractions of non-vascular smooth muscle. Methods: We isolated WT and L144F SOD1 enzymes from eight patients with FALS, L144F mutation in exon 5 and eight healthy controls. We then investigated SOD1 activities in the obtained samples by the adrenaline method and profiled them electrophoretically. Finally, we applied WT and L144F SOD1 on the isolated rat uterus. Results: L144F SOD1 showed lower superoxide-dismutating activity compared to WT human SOD1. We found that, in contrast to WT human SOD1, mutated L144F does not induce smooth muscle relaxation. Conclusions: Our data suggest that the lack of relaxation of muscle tonus in the presence of mutated SOD1 may have pathogenic feedback effects in FALS.
AB  - Uvod: Mutirana bakar, cink superoksid-dizmutaza (SOD1) može da pravi agregate, sto predstavlja početni uzrok oštećenja motornog neurona može da izazove nastanak bolesti. U ovom radu su pokazani efekti humane bakar, cink super-oksid dizmutaze iz krvi pacijenata obolelih od familijarne amiotrofične lateralne skleroze (FALS) sa Leu144Phe (L144F) mutacijom i normalne (wild-type - WT) humane SOD1, iz krvi zdravih kontrola, na glatkom mišiću. Metode: Izolovali smo WT i L144F SOD1 enzime kod osam odabranih FALS pacijenata sa L144F mutacijom na egzonu 5 i pet zdravih kontrola. Dalje smo ispitivali aktivnost SOD1 u dobijenim uzorcima adrenalinskom metodom i elektro-foretski ih profilisali. Konačno, izolovanu WT i L144F SOD1 aplicirali smo na izolovani uterus pacova. Rezultati: Aktivnost L144F SOD1 je statistički značajno manja (p<0,05) u poređenju sa aktivnosti WT SOD1 zdravih kontrola. L144F ne izaziva relaksaciju glatkog mišića, kao sto je to slučaj sa WT SOD1. Zaključak: Naši rezultati pokazuju da izostanak relaksacije mišićnog tonusa u prisustvu mutirane SOD1 može imati štetni povratni efekat kod FALS pacijenata.
T2  - Journal of Medical Biochemistry
T1  - Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića
T1  - The effects of human wild-type and FALS mutant L144P SOD1 on non-vascular smooth muscle contractions
IS  - 4
VL  - 32
SP  - 375
EP  - 379
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_504
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Blagojević, Duško and Oreščanin Dušić, Zorana and Slavić, Marija and Spasojević, Ivan and Stević, Zorica and Spasić, Mihajlo",
year = "2013, 2013",
abstract = "Background: Mutated copper, zinc-containing superoxide dismutase (SOD1) may self-aggregate, an event that could also be an initial cause of motor neuron malfunction leading to disease onset. The effects of human mutated SOD1 protein from the blood of familial amyotrophic lateral sclerosis (FALS) patients bearing Leu144Phe (L144F) mutation were compared to wild-type (WT) human SOD1 derived from healthy examinees, for enzymatic activity and the effects on isometric contractions of non-vascular smooth muscle. Methods: We isolated WT and L144F SOD1 enzymes from eight patients with FALS, L144F mutation in exon 5 and eight healthy controls. We then investigated SOD1 activities in the obtained samples by the adrenaline method and profiled them electrophoretically. Finally, we applied WT and L144F SOD1 on the isolated rat uterus. Results: L144F SOD1 showed lower superoxide-dismutating activity compared to WT human SOD1. We found that, in contrast to WT human SOD1, mutated L144F does not induce smooth muscle relaxation. Conclusions: Our data suggest that the lack of relaxation of muscle tonus in the presence of mutated SOD1 may have pathogenic feedback effects in FALS., Uvod: Mutirana bakar, cink superoksid-dizmutaza (SOD1) može da pravi agregate, sto predstavlja početni uzrok oštećenja motornog neurona može da izazove nastanak bolesti. U ovom radu su pokazani efekti humane bakar, cink super-oksid dizmutaze iz krvi pacijenata obolelih od familijarne amiotrofične lateralne skleroze (FALS) sa Leu144Phe (L144F) mutacijom i normalne (wild-type - WT) humane SOD1, iz krvi zdravih kontrola, na glatkom mišiću. Metode: Izolovali smo WT i L144F SOD1 enzime kod osam odabranih FALS pacijenata sa L144F mutacijom na egzonu 5 i pet zdravih kontrola. Dalje smo ispitivali aktivnost SOD1 u dobijenim uzorcima adrenalinskom metodom i elektro-foretski ih profilisali. Konačno, izolovanu WT i L144F SOD1 aplicirali smo na izolovani uterus pacova. Rezultati: Aktivnost L144F SOD1 je statistički značajno manja (p<0,05) u poređenju sa aktivnosti WT SOD1 zdravih kontrola. L144F ne izaziva relaksaciju glatkog mišića, kao sto je to slučaj sa WT SOD1. Zaključak: Naši rezultati pokazuju da izostanak relaksacije mišićnog tonusa u prisustvu mutirane SOD1 može imati štetni povratni efekat kod FALS pacijenata.",
journal = "Journal of Medical Biochemistry",
title = "Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića, The effects of human wild-type and FALS mutant L144P SOD1 on non-vascular smooth muscle contractions",
number = "4",
volume = "32",
pages = "375-379",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_504"
}

Nikolić-Kokić, A., Blagojević, D., Oreščanin Dušić, Z., Slavić, M., Spasojević, I., Stević, Z.,& Spasić, M.. (2013). Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića. in Journal of Medical Biochemistry, 32(4), 375-379.
https://hdl.handle.net/21.15107/rcub_ibiss_504

Nikolić-Kokić A, Blagojević D, Oreščanin Dušić Z, Slavić M, Spasojević I, Stević Z, Spasić M. Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića. in Journal of Medical Biochemistry. 2013;32(4):375-379.
https://hdl.handle.net/21.15107/rcub_ibiss_504 .

Nikolić-Kokić, Aleksandra, Blagojević, Duško, Oreščanin Dušić, Zorana, Slavić, Marija, Spasojević, Ivan, Stević, Zorica, Spasić, Mihajlo, "Efekti humane normalne i FALS mutirane L144P SOD1 na nevaskularne kontrakcije glatkih mišića" in Journal of Medical Biochemistry, 32, no. 4 (2013):375-379,
https://hdl.handle.net/21.15107/rcub_ibiss_504 .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Spasojević, Ivan
AU  - Živanović, Jasmina
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Milošević, Verica
AU  - Severs, Walter
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/498
AB  - Erythrocyte membrane fluidity is related to their rheologic behavior, the dynamic quality of erythrocytes, which is tempted in hypertension and atherosclerosis. An increased risk of these and other cardiovascular diseases occurs in ageing women. Menopause-related conditions are often treated with hormone replacement therapy that may increase the risk of malignancies. Vitex agnus-castus L. essential oil contains various organic compounds (monoterpenes, sesquiterpenes and terpenoids), and is increasingly used as an alternative therapy for menopausal symptoms. These components of the oil may be incorporated into cell membranes, thereby changing the membrane fluidity. The aim of this study was to determine the effects of Vitex agnuscastus essential oil on human erythrocyte membrane fluidity at graded depths. We used Electron Paramagnetic Resonance spectroscopy and fatty acid spin probes (5-doxyl stearic acid and 12-doxyl stearic acid), whose spectra depend on membrane fluidity. After treatment with Vitex agnus-castus essential oil the erythrocytes had a significant (p=0.029) and reversible increase in membrane fluidity in the deeper hydrophobic membrane regions, with no change (p>0.05) in fluidity near the membrane's hydrophilic surface. These results document increased fluidity of the human erythrocyte membrane by Vitex agnus-castus essential oil, and this action may be useful in patients with menopause-related hypertension and other cardiovascular conditions.
AB  - Fluidnost membrane eritrocita uslovljava njihove reološke karakteristike, svojevrsne dinamičke kvalitete, izmenjene u hipertenziji i aterosklerozi. Rizik od navedenih i drugih kardiovaskularnih oboljenja se kod žena povećava sa starenjem. Tegobe koje prate menopauzu se obično ublažavaju supstitucionom hormonskom terapijom, koja može povećati rizik od pojave malignih oboljenja. Esencijalno ulje biljke Vitex agnus-castus L. sadrži različita organska jedinjenja (monoterpene, seskviterpene i terpenoide) i sve češće se koristi kao alternativno terapijsko sredstvo za simptome menopauze. Navedene komponente esencijalnog ulja mogu se ugraditi u ćelijske membrane i menjati njihovu fluidnost. Cilj studije bio je da se utvrde efekti esencijalnog ulja biljke Vitex agnus-castus na fluidnost membrane eritrocita po njenoj du bini. Korišćena je spektroskopija elektronske paramagnetne rezonance i masnokiselinske spinske probe (5-doksil stearinska i 12-doksil stearinska kiselina), čiji spektri za vise od fluidnosti membrane. Nakon tretmana eritrocita esencijalnim uljem biljke Vitex agnus-castus utvrđeno je značajno (p=0,029) i reverzibilno povećanje fluidnosti njihove membrane u dubljim, hidrofobnim regionima, dok u površinskim, hidrofilnim regionima eritrocitne membrane nisu utvrđene značajne promene (p>0,05). Ovi rezultati ukazuju na povećanje fluidnosti eritrocitne membrane nakon tretmana esencijalnim uljem biljke Vitex agnus-castus, što može biti koristan efekat pri tretmanu pacijentkinja sa hipertenzijom i drugim kardiovaskularnim oboljenjima u menopauzi.
PB  - Belgrade: Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane
T1  - Vitex agnus-castus L.: Essential oil increases human erythrocyte membrane fluidity
IS  - 3
VL  - 31
DO  - 10.2478/v10011-011-0055-x
SP  - 222
EP  - 227
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_498
ER  - 

@article{
author = "Ajdžanović, Vladimir and Spasojević, Ivan and Živanović, Jasmina and Šošić-Jurjević, Branka  and Filipović, Branko and Milošević, Verica and Severs, Walter",
year = "2012, 2012",
abstract = "Erythrocyte membrane fluidity is related to their rheologic behavior, the dynamic quality of erythrocytes, which is tempted in hypertension and atherosclerosis. An increased risk of these and other cardiovascular diseases occurs in ageing women. Menopause-related conditions are often treated with hormone replacement therapy that may increase the risk of malignancies. Vitex agnus-castus L. essential oil contains various organic compounds (monoterpenes, sesquiterpenes and terpenoids), and is increasingly used as an alternative therapy for menopausal symptoms. These components of the oil may be incorporated into cell membranes, thereby changing the membrane fluidity. The aim of this study was to determine the effects of Vitex agnuscastus essential oil on human erythrocyte membrane fluidity at graded depths. We used Electron Paramagnetic Resonance spectroscopy and fatty acid spin probes (5-doxyl stearic acid and 12-doxyl stearic acid), whose spectra depend on membrane fluidity. After treatment with Vitex agnus-castus essential oil the erythrocytes had a significant (p=0.029) and reversible increase in membrane fluidity in the deeper hydrophobic membrane regions, with no change (p>0.05) in fluidity near the membrane's hydrophilic surface. These results document increased fluidity of the human erythrocyte membrane by Vitex agnus-castus essential oil, and this action may be useful in patients with menopause-related hypertension and other cardiovascular conditions., Fluidnost membrane eritrocita uslovljava njihove reološke karakteristike, svojevrsne dinamičke kvalitete, izmenjene u hipertenziji i aterosklerozi. Rizik od navedenih i drugih kardiovaskularnih oboljenja se kod žena povećava sa starenjem. Tegobe koje prate menopauzu se obično ublažavaju supstitucionom hormonskom terapijom, koja može povećati rizik od pojave malignih oboljenja. Esencijalno ulje biljke Vitex agnus-castus L. sadrži različita organska jedinjenja (monoterpene, seskviterpene i terpenoide) i sve češće se koristi kao alternativno terapijsko sredstvo za simptome menopauze. Navedene komponente esencijalnog ulja mogu se ugraditi u ćelijske membrane i menjati njihovu fluidnost. Cilj studije bio je da se utvrde efekti esencijalnog ulja biljke Vitex agnus-castus na fluidnost membrane eritrocita po njenoj du bini. Korišćena je spektroskopija elektronske paramagnetne rezonance i masnokiselinske spinske probe (5-doksil stearinska i 12-doksil stearinska kiselina), čiji spektri za vise od fluidnosti membrane. Nakon tretmana eritrocita esencijalnim uljem biljke Vitex agnus-castus utvrđeno je značajno (p=0,029) i reverzibilno povećanje fluidnosti njihove membrane u dubljim, hidrofobnim regionima, dok u površinskim, hidrofilnim regionima eritrocitne membrane nisu utvrđene značajne promene (p>0,05). Ovi rezultati ukazuju na povećanje fluidnosti eritrocitne membrane nakon tretmana esencijalnim uljem biljke Vitex agnus-castus, što može biti koristan efekat pri tretmanu pacijentkinja sa hipertenzijom i drugim kardiovaskularnim oboljenjima u menopauzi.",
publisher = "Belgrade: Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane, Vitex agnus-castus L.: Essential oil increases human erythrocyte membrane fluidity",
number = "3",
volume = "31",
doi = "10.2478/v10011-011-0055-x",
pages = "222-227",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_498"
}

Ajdžanović, V., Spasojević, I., Živanović, J., Šošić-Jurjević, B., Filipović, B., Milošević, V.,& Severs, W.. (2012). Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane. in Journal of Medical Biochemistry
Belgrade: Society of Medical Biochemists of Serbia., 31(3), 222-227.
https://doi.org/10.2478/v10011-011-0055-x
https://hdl.handle.net/21.15107/rcub_ibiss_498

Ajdžanović V, Spasojević I, Živanović J, Šošić-Jurjević B, Filipović B, Milošević V, Severs W. Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane. in Journal of Medical Biochemistry. 2012;31(3):222-227.
doi:10.2478/v10011-011-0055-x
https://hdl.handle.net/21.15107/rcub_ibiss_498 .

Ajdžanović, Vladimir, Spasojević, Ivan, Živanović, Jasmina, Šošić-Jurjević, Branka , Filipović, Branko, Milošević, Verica, Severs, Walter, "Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane" in Journal of Medical Biochemistry, 31, no. 3 (2012):222-227,
https://doi.org/10.2478/v10011-011-0055-x .,
https://hdl.handle.net/21.15107/rcub_ibiss_498 .