TY  - JOUR
AU  - Krizak, Strahinja
AU  - Nikoic, Ljiljana
AU  - Stanic, Marina
AU  - Zizic, Milan
AU  - Zakrzewska, Joanna
AU  - Zivic, Miroslav
AU  - Todorović, Nataša
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1977
AB  - We describe here whole-cell currents of droplets prepared from the
   apical region of growing Phycomyces blakesleeanus sporangiophores.
   Whole-cell current recordings revealed the osmotically activated,
   outwardly rectifying, fast inactivating instantaneous current (ORIC)
   with biophysical properties closely resembling volume-regulated anionic
   current (VRAC). ORIC is activated under conditions of osmotically
   induced swelling and shows strong selectivity for anions over cations.
   In addition, ORIC shows voltage and time-dependent inactivation at
   positive potentials and recovery from inactivation at negative
   potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion
   channel blocker, in a voltage-dependent manner. This is the first report
   of the presence of VRAC-like current in an organism outside the chordate
   lineage. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS.
   All rights reserved.
T2  - Research in Microbiology
T1  - Osmotic swelling activates a novel anionic current with VRAC-like
 properties in a cytoplasmic droplet membrane from Phycomyces
 blakesleeanus sporangiophores
IS  - 3
VL  - 166
DO  - 10.1016/j.resmic.2015.02.004
SP  - 162
EP  - 173
ER  - 

@article{
author = "Krizak, Strahinja and Nikoic, Ljiljana and Stanic, Marina and Zizic, Milan and Zakrzewska, Joanna and Zivic, Miroslav and Todorović, Nataša",
year = "2015",
abstract = "We describe here whole-cell currents of droplets prepared from the
   apical region of growing Phycomyces blakesleeanus sporangiophores.
   Whole-cell current recordings revealed the osmotically activated,
   outwardly rectifying, fast inactivating instantaneous current (ORIC)
   with biophysical properties closely resembling volume-regulated anionic
   current (VRAC). ORIC is activated under conditions of osmotically
   induced swelling and shows strong selectivity for anions over cations.
   In addition, ORIC shows voltage and time-dependent inactivation at
   positive potentials and recovery from inactivation at negative
   potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion
   channel blocker, in a voltage-dependent manner. This is the first report
   of the presence of VRAC-like current in an organism outside the chordate
   lineage. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS.
   All rights reserved.",
journal = "Research in Microbiology",
title = "Osmotic swelling activates a novel anionic current with VRAC-like
 properties in a cytoplasmic droplet membrane from Phycomyces
 blakesleeanus sporangiophores",
number = "3",
volume = "166",
doi = "10.1016/j.resmic.2015.02.004",
pages = "162-173"
}

Krizak, S., Nikoic, L., Stanic, M., Zizic, M., Zakrzewska, J., Zivic, M.,& Todorović, N.. (2015). Osmotic swelling activates a novel anionic current with VRAC-like
 properties in a cytoplasmic droplet membrane from Phycomyces
 blakesleeanus sporangiophores. in Research in Microbiology, 166(3), 162-173.
https://doi.org/10.1016/j.resmic.2015.02.004

Krizak S, Nikoic L, Stanic M, Zizic M, Zakrzewska J, Zivic M, Todorović N. Osmotic swelling activates a novel anionic current with VRAC-like
 properties in a cytoplasmic droplet membrane from Phycomyces
 blakesleeanus sporangiophores. in Research in Microbiology. 2015;166(3):162-173.
doi:10.1016/j.resmic.2015.02.004 .

Krizak, Strahinja, Nikoic, Ljiljana, Stanic, Marina, Zizic, Milan, Zakrzewska, Joanna, Zivic, Miroslav, Todorović, Nataša, "Osmotic swelling activates a novel anionic current with VRAC-like
 properties in a cytoplasmic droplet membrane from Phycomyces
 blakesleeanus sporangiophores" in Research in Microbiology, 166, no. 3 (2015):162-173,
https://doi.org/10.1016/j.resmic.2015.02.004 . .

TY  - JOUR
AU  - Mojić, Marija
AU  - Bogdanović Pristov, Jelena
AU  - Maksimović-Ivanić, Danijela
AU  - Jones, David R.
AU  - Stanic, Marina
AU  - Mijatović, Sanja
AU  - Spasojevic, Ivan
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2177
AB  - In vitro studies have shown that hydrogen peroxide (H2O2) produced by
   high-concentration ascorbate and cell culture medium iron efficiently
   kills cancer cells. This provided the rationale for clinical trials of
   high-dose intravenous ascorbate-based treatment for cancer. A drawback
   in all the in vitro studies was their failure to take into account the
   in vivo concentration of iron to supplement cell culture media which are
   characterized by low iron content. Here we showed, using two prostate
   cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the
   anticancer/cytotoxic effects of ascorbate are completely abolished by
   iron at physiological concentrations in cell culture medium and human
   plasma. A detailed examination of mechanisms showed that iron at
   physiological concentrations promotes both production and decomposition
   of H2O2. The latter is mediated by Fenton reaction and prevents H2O2
   accumulation. The hydroxyl radical, which is produced in the Fenton
   reaction, is buffered by extracellular proteins, and could not affect
   intracellular targets like H2O2. These findings show that anticancer
   effects of ascorbate have been significantly overestimated in previous
   in vitro studies, and that common cell culture media might be unsuitable
   for redox research.
T2  - Scientific Reports
T1  - Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study
IS  - 5955
VL  - 4
DO  - 10.1038/srep05955
ER  - 

@article{
author = "Mojić, Marija and Bogdanović Pristov, Jelena and Maksimović-Ivanić, Danijela and Jones, David R. and Stanic, Marina and Mijatović, Sanja and Spasojevic, Ivan",
year = "2014",
abstract = "In vitro studies have shown that hydrogen peroxide (H2O2) produced by
   high-concentration ascorbate and cell culture medium iron efficiently
   kills cancer cells. This provided the rationale for clinical trials of
   high-dose intravenous ascorbate-based treatment for cancer. A drawback
   in all the in vitro studies was their failure to take into account the
   in vivo concentration of iron to supplement cell culture media which are
   characterized by low iron content. Here we showed, using two prostate
   cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the
   anticancer/cytotoxic effects of ascorbate are completely abolished by
   iron at physiological concentrations in cell culture medium and human
   plasma. A detailed examination of mechanisms showed that iron at
   physiological concentrations promotes both production and decomposition
   of H2O2. The latter is mediated by Fenton reaction and prevents H2O2
   accumulation. The hydroxyl radical, which is produced in the Fenton
   reaction, is buffered by extracellular proteins, and could not affect
   intracellular targets like H2O2. These findings show that anticancer
   effects of ascorbate have been significantly overestimated in previous
   in vitro studies, and that common cell culture media might be unsuitable
   for redox research.",
journal = "Scientific Reports",
title = "Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study",
number = "5955",
volume = "4",
doi = "10.1038/srep05955"
}

Mojić, M., Bogdanović Pristov, J., Maksimović-Ivanić, D., Jones, D. R., Stanic, M., Mijatović, S.,& Spasojevic, I.. (2014). Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study. in Scientific Reports, 4(5955).
https://doi.org/10.1038/srep05955

Mojić M, Bogdanović Pristov J, Maksimović-Ivanić D, Jones DR, Stanic M, Mijatović S, Spasojevic I. Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study. in Scientific Reports. 2014;4(5955).
doi:10.1038/srep05955 .

Mojić, Marija, Bogdanović Pristov, Jelena, Maksimović-Ivanić, Danijela, Jones, David R., Stanic, Marina, Mijatović, Sanja, Spasojevic, Ivan, "Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study" in Scientific Reports, 4, no. 5955 (2014),
https://doi.org/10.1038/srep05955 . .