TY  - JOUR
AU  - Ristić, Nataša
AU  - Ajdžanović, Vladimir
AU  - Petrović-Kosanović, Dragana
AU  - Miler, Marko
AU  - Ušćebrka, Gordana
AU  - Milošević, Verica
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3168
AB  - Andropause, the culminating phase of male ageing, is characterized by deregulation of the hypothalamic-pituitarygonadal axis and low circulating free testosterone. The aim of this study was to investigate the immunohistomorphometric characteristics of the pituitary gonadotropic i.e. follicle-stimulating hormone (FSH) and the luteinizing hormone (LH) producing cells after testosterone application in a rat model of the andropause. Middle-aged Wistar rats were divided into orchidectomized (ORX; n=8) and testosterone treated orchidectomized (ORX+T; n=8) groups. Testosterone propionate (5 mg/kg b.m. /day) was administered for three weeks, while the ORX group received the vehicle alone. Immunohistochemically stained FSH and LH cells underwent morphometric and optical density-related analysis, while circulating concentrations of the sex steroids were measured by immunoassays. Serum concentrations of testosterone and estradiol were significantly (p<0.05) increased by 24 and 2.7 fold respectively, compared to the ORX group. The volume of FSH and LH cells was significantly (p<0.05) decreased by 51.3% and 56.6% respectively, in comparison with ORX rats. Relative volume density of FSH and LH cells was also significantly (p<0.05) decreased by 54.0% and 72.8% respectively, compared to the ORX group. Results related to the optical density of gonadotropic cells (reflecting their hormonal content) were in line with the morphometric findings i.e. this parameter of FSH and LH cells was significantly (p<0.05) decreased by 25.7% and 16.2% respectively, in comparison with ORX rats. Conclusion: In conclusion, applied testosterone increased the serum concentrations of sex steroids, as well as it decreased morphometric parameters and optical density of gonadotropic cells in ORX rats.
T2  - Macedonian Veterinary Review
T1  - Immunohistomorphometric changes of the pituitary gonadotropic cells after testosterone application in a rat model of the andropause
IS  - 1
VL  - 42
DO  - 10.2478/macvetrev-2018-0023
SP  - 5
EP  - 13
ER  - 

@article{
author = "Ristić, Nataša and Ajdžanović, Vladimir and Petrović-Kosanović, Dragana and Miler, Marko and Ušćebrka, Gordana and Milošević, Verica",
year = "2019",
abstract = "Andropause, the culminating phase of male ageing, is characterized by deregulation of the hypothalamic-pituitarygonadal axis and low circulating free testosterone. The aim of this study was to investigate the immunohistomorphometric characteristics of the pituitary gonadotropic i.e. follicle-stimulating hormone (FSH) and the luteinizing hormone (LH) producing cells after testosterone application in a rat model of the andropause. Middle-aged Wistar rats were divided into orchidectomized (ORX; n=8) and testosterone treated orchidectomized (ORX+T; n=8) groups. Testosterone propionate (5 mg/kg b.m. /day) was administered for three weeks, while the ORX group received the vehicle alone. Immunohistochemically stained FSH and LH cells underwent morphometric and optical density-related analysis, while circulating concentrations of the sex steroids were measured by immunoassays. Serum concentrations of testosterone and estradiol were significantly (p<0.05) increased by 24 and 2.7 fold respectively, compared to the ORX group. The volume of FSH and LH cells was significantly (p<0.05) decreased by 51.3% and 56.6% respectively, in comparison with ORX rats. Relative volume density of FSH and LH cells was also significantly (p<0.05) decreased by 54.0% and 72.8% respectively, compared to the ORX group. Results related to the optical density of gonadotropic cells (reflecting their hormonal content) were in line with the morphometric findings i.e. this parameter of FSH and LH cells was significantly (p<0.05) decreased by 25.7% and 16.2% respectively, in comparison with ORX rats. Conclusion: In conclusion, applied testosterone increased the serum concentrations of sex steroids, as well as it decreased morphometric parameters and optical density of gonadotropic cells in ORX rats.",
journal = "Macedonian Veterinary Review",
title = "Immunohistomorphometric changes of the pituitary gonadotropic cells after testosterone application in a rat model of the andropause",
number = "1",
volume = "42",
doi = "10.2478/macvetrev-2018-0023",
pages = "5-13"
}

Ristić, N., Ajdžanović, V., Petrović-Kosanović, D., Miler, M., Ušćebrka, G.,& Milošević, V.. (2019). Immunohistomorphometric changes of the pituitary gonadotropic cells after testosterone application in a rat model of the andropause. in Macedonian Veterinary Review, 42(1), 5-13.
https://doi.org/10.2478/macvetrev-2018-0023

Ristić N, Ajdžanović V, Petrović-Kosanović D, Miler M, Ušćebrka G, Milošević V. Immunohistomorphometric changes of the pituitary gonadotropic cells after testosterone application in a rat model of the andropause. in Macedonian Veterinary Review. 2019;42(1):5-13.
doi:10.2478/macvetrev-2018-0023 .

Ristić, Nataša, Ajdžanović, Vladimir, Petrović-Kosanović, Dragana, Miler, Marko, Ušćebrka, Gordana, Milošević, Verica, "Immunohistomorphometric changes of the pituitary gonadotropic cells after testosterone application in a rat model of the andropause" in Macedonian Veterinary Review, 42, no. 1 (2019):5-13,
https://doi.org/10.2478/macvetrev-2018-0023 . .

TY  - CONF
AU  - Petrović-Kosanović, Dragana
AU  - Šošić-Jurjević, Branka
AU  - Veličković, Ksenija
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Ajdžanović, Vladimir
AU  - Jasnić, Nebojša
PY  - 2014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6245
AB  - Hyperthermia induced by heat stress cause damages to various organs. Data on the effects of heat stress on thyroid gland are scarce, despite the fact that thyroid hormones (TH) play essential role in maintenance of thermogenesis and basal metabolic rate. In this study we examined effects of acute heat stress on thyroid histology and concentration of TH and thyrotropin (TSH) in sera. Adult male Wistar rats were divided in two groups (n=10): the first group were intact controls; the other group was exposed to ambient temperature of 30°C for 60 min in a hot chamber. Animals were decapitated immediately after exposure to heat stress. Thyroid glands were excised, weighed and prepared for further histo-morphometric analyses. Serum concentrations of TH were determined by RIA, while TSH was determined by ELISA. Acute exposure to ambient temperature of 38°C did not induce damages of thyroid tissue. Morphometric analysis revealed decreased volume density of thyroid follicles (p<0.05), while the volume density of blood vessels increased (p<0.05). Besides dilation of blood vessels, vascular congestion was also evident. Serum concentrations of TH were unchanged, while TSH increased (p<0.05). Acute exposure to moderate heat stress did not result in injuries of thyroid tissue. Still, increased volume density of blood vessels and accumulation of erythrocytes indicate thyroid blood flow. Increased blood flow, together with increased serum TSH probably contributed to increased mobilization of colloid and thyroid hormone stores under these conditions.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia
T1  - The effect of acute heat stress on rat pituitary-thyroid axis
SP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6245
ER  - 

@conference{
author = "Petrović-Kosanović, Dragana and Šošić-Jurjević, Branka and Veličković, Ksenija and Nestorović, Nataša and Ristić, Nataša and Ajdžanović, Vladimir and Jasnić, Nebojša",
year = "2014",
abstract = "Hyperthermia induced by heat stress cause damages to various organs. Data on the effects of heat stress on thyroid gland are scarce, despite the fact that thyroid hormones (TH) play essential role in maintenance of thermogenesis and basal metabolic rate. In this study we examined effects of acute heat stress on thyroid histology and concentration of TH and thyrotropin (TSH) in sera. Adult male Wistar rats were divided in two groups (n=10): the first group were intact controls; the other group was exposed to ambient temperature of 30°C for 60 min in a hot chamber. Animals were decapitated immediately after exposure to heat stress. Thyroid glands were excised, weighed and prepared for further histo-morphometric analyses. Serum concentrations of TH were determined by RIA, while TSH was determined by ELISA. Acute exposure to ambient temperature of 38°C did not induce damages of thyroid tissue. Morphometric analysis revealed decreased volume density of thyroid follicles (p<0.05), while the volume density of blood vessels increased (p<0.05). Besides dilation of blood vessels, vascular congestion was also evident. Serum concentrations of TH were unchanged, while TSH increased (p<0.05). Acute exposure to moderate heat stress did not result in injuries of thyroid tissue. Still, increased volume density of blood vessels and accumulation of erythrocytes indicate thyroid blood flow. Increased blood flow, together with increased serum TSH probably contributed to increased mobilization of colloid and thyroid hormone stores under these conditions.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia",
title = "The effect of acute heat stress on rat pituitary-thyroid axis",
pages = "160",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6245"
}

Petrović-Kosanović, D., Šošić-Jurjević, B., Veličković, K., Nestorović, N., Ristić, N., Ajdžanović, V.,& Jasnić, N.. (2014). The effect of acute heat stress on rat pituitary-thyroid axis. in Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 160.
https://hdl.handle.net/21.15107/rcub_ibiss_6245

Petrović-Kosanović D, Šošić-Jurjević B, Veličković K, Nestorović N, Ristić N, Ajdžanović V, Jasnić N. The effect of acute heat stress on rat pituitary-thyroid axis. in Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia. 2014;:160.
https://hdl.handle.net/21.15107/rcub_ibiss_6245 .

Petrović-Kosanović, Dragana, Šošić-Jurjević, Branka, Veličković, Ksenija, Nestorović, Nataša, Ristić, Nataša, Ajdžanović, Vladimir, Jasnić, Nebojša, "The effect of acute heat stress on rat pituitary-thyroid axis" in Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia (2014):160,
https://hdl.handle.net/21.15107/rcub_ibiss_6245 .

TY  - JOUR
AU  - Petrović-Kosanović, Dragana
AU  - Ajdžanović, Vladimir
AU  - Čakić-Milošević, Maja
AU  - Koko, Vesna
AU  - Milošević, Verica
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/346
AB  - Hyperthermia can cause significant structural and functional reorganization of tissues and organs. The prolifera- tive and apoptotic processes of rat adrenal cortex were analyzed by light and electron microscopy after an acute exposure to high ambient temperature. Animals were divided in two groups. The first group consisted of intact controls. The rats from the second group were exposed to a high ambient temperature of 38°C for 60 min. Mitotic chromosomes and the largest number of immunoreactive nuclei for the Ki-67 were observed in the zona reticularis (ZR) of the control animals. The relative number of mitoses after heat stress showed a significant decrease in the zona glomerulosa (ZG; 66.8%), zona fasciculata (ZF; 27.8%) and ZR (86.7%) (for all zones p<0.05), while in the whole adrenal cortex the after-treatment decrease was 61.9% (p<0.05) compared to the controls. Under heat stress numerous apoptotic nuclei were seen at the light and ultrastructural levels in all the zones of the adrenal cortex. Such dynamics of mitosis/apoptosis events seriously affect adrenal cortex morphology.
T2  - Archives of Biological Sciences
T1  - The effects of acute heat stress on proliferative and apoptotic processes in the rat adrenal cortex
IS  - 3
VL  - 65
SP  - 905
EP  - 909
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_346
ER  - 

@article{
author = "Petrović-Kosanović, Dragana and Ajdžanović, Vladimir and Čakić-Milošević, Maja and Koko, Vesna and Milošević, Verica",
year = "2013, 2013",
abstract = "Hyperthermia can cause significant structural and functional reorganization of tissues and organs. The prolifera- tive and apoptotic processes of rat adrenal cortex were analyzed by light and electron microscopy after an acute exposure to high ambient temperature. Animals were divided in two groups. The first group consisted of intact controls. The rats from the second group were exposed to a high ambient temperature of 38°C for 60 min. Mitotic chromosomes and the largest number of immunoreactive nuclei for the Ki-67 were observed in the zona reticularis (ZR) of the control animals. The relative number of mitoses after heat stress showed a significant decrease in the zona glomerulosa (ZG; 66.8%), zona fasciculata (ZF; 27.8%) and ZR (86.7%) (for all zones p<0.05), while in the whole adrenal cortex the after-treatment decrease was 61.9% (p<0.05) compared to the controls. Under heat stress numerous apoptotic nuclei were seen at the light and ultrastructural levels in all the zones of the adrenal cortex. Such dynamics of mitosis/apoptosis events seriously affect adrenal cortex morphology.",
journal = "Archives of Biological Sciences",
title = "The effects of acute heat stress on proliferative and apoptotic processes in the rat adrenal cortex",
number = "3",
volume = "65",
pages = "905-909",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_346"
}

Petrović-Kosanović, D., Ajdžanović, V., Čakić-Milošević, M., Koko, V.,& Milošević, V.. (2013). The effects of acute heat stress on proliferative and apoptotic processes in the rat adrenal cortex. in Archives of Biological Sciences, 65(3), 905-909.
https://hdl.handle.net/21.15107/rcub_ibiss_346

Petrović-Kosanović D, Ajdžanović V, Čakić-Milošević M, Koko V, Milošević V. The effects of acute heat stress on proliferative and apoptotic processes in the rat adrenal cortex. in Archives of Biological Sciences. 2013;65(3):905-909.
https://hdl.handle.net/21.15107/rcub_ibiss_346 .

Petrović-Kosanović, Dragana, Ajdžanović, Vladimir, Čakić-Milošević, Maja, Koko, Vesna, Milošević, Verica, "The effects of acute heat stress on proliferative and apoptotic processes in the rat adrenal cortex" in Archives of Biological Sciences, 65, no. 3 (2013):905-909,
https://hdl.handle.net/21.15107/rcub_ibiss_346 .

TY  - JOUR
AU  - Petrović-Kosanović, Dragana
AU  - Ukropina, Mirela
AU  - Čakić-Milošević, Maja
AU  - Budeč, Mirela
AU  - Milošević, Verica
AU  - Koko, Vesna
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/329
AB  - Immunohistochemistry revealed the presence of Vasoactive Intestinal Peptide (VIP), Neuropeptide Y (NPY), and the absence of Substance P (SP) immunoreactivity in the rat adrenal cortex. VIP- and NPY-immunoreactivity were detected in nerve fibers around the small blood vessels projecting into the capsule and cortical zones surrounding blood vessels and cortical cells. After acute heat stress, VIP- and NPY-immunoreactivities in the nerve fibers were reduced, probably as a result of the release of these peptides.
T2  - Archives of Biological Sciences
T1  - Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress
IS  - 1
VL  - 65
SP  - 315
EP  - 320
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_329
ER  - 

@article{
author = "Petrović-Kosanović, Dragana and Ukropina, Mirela and Čakić-Milošević, Maja and Budeč, Mirela and Milošević, Verica and Koko, Vesna",
year = "2013, 2013",
abstract = "Immunohistochemistry revealed the presence of Vasoactive Intestinal Peptide (VIP), Neuropeptide Y (NPY), and the absence of Substance P (SP) immunoreactivity in the rat adrenal cortex. VIP- and NPY-immunoreactivity were detected in nerve fibers around the small blood vessels projecting into the capsule and cortical zones surrounding blood vessels and cortical cells. After acute heat stress, VIP- and NPY-immunoreactivities in the nerve fibers were reduced, probably as a result of the release of these peptides.",
journal = "Archives of Biological Sciences",
title = "Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress",
number = "1",
volume = "65",
pages = "315-320",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_329"
}

Petrović-Kosanović, D., Ukropina, M., Čakić-Milošević, M., Budeč, M., Milošević, V.,& Koko, V.. (2013). Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress. in Archives of Biological Sciences, 65(1), 315-320.
https://hdl.handle.net/21.15107/rcub_ibiss_329

Petrović-Kosanović D, Ukropina M, Čakić-Milošević M, Budeč M, Milošević V, Koko V. Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress. in Archives of Biological Sciences. 2013;65(1):315-320.
https://hdl.handle.net/21.15107/rcub_ibiss_329 .

Petrović-Kosanović, Dragana, Ukropina, Mirela, Čakić-Milošević, Maja, Budeč, Mirela, Milošević, Verica, Koko, Vesna, "Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress" in Archives of Biological Sciences, 65, no. 1 (2013):315-320,
https://hdl.handle.net/21.15107/rcub_ibiss_329 .