TY  - JOUR
AU  - De Oliveira, Izamara
AU  - Chrysargyris, Antonios
AU  - Heleno, Sandrina A.
AU  - Carocho, Marcio
AU  - Calhelha, Ricardo
AU  - Dias, Maria Ines
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Petropoulos, Spyridon
AU  - Santos-Buelga, Celestino
AU  - Tzortzakis, Nikolaos
AU  - Barros, Lillian
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6389
AB  - The present study aims to determine the combined effect of cropping system and irrigation regime on the chemical composition and bioactive properties of lemon balm aerial parts. For this purpose, lemon balm plants were grown under two farming systems (conventional farming vs organic farming) and two irrigation levels (full irrigation vs deficit irrigation) and harvested twice throughout the growing period. The collected aerial parts were subjected to three different methods of extractions, namely infusion, maceration and ultrasound-assisted extraction and the obtained extracts were evaluated in terms of chemical profile and bioactivities. Five organic acids with varied composition among the tested treatments were identified in all the tested samples for both harvests, namely, citric, malic, oxalic, shikimic and quinic acid. Regarding phenolic compounds composition, the most abundant ones were rosmarinic acid, lithospermic acid A isomer I and hydroxylsalvianolic E, especially for the maceration and infusion extraction methods. Full irrigation resulted in lower EC50 values than deficit irrigation only in the treatments of the second harvest, while variable cytotoxic and anti-inflammatory effects were recorded in both harvests. Finally, in most cases the lemon balm extracts has similar or better activity than the positive controls, while the antifungal activity of lemon balm extracts was stronger than the antibacterial effects. In conclusion, the results of the present study showed that the implemented agronomic practices, as well as the extraction protocol may significantly affect the chemical profile and bioactivities of lemon balm extracts, suggesting that both the farming system and the irrigation schedule may improve the quality of the extracts depending on the implemented extraction protocol.
PB  - Elsevier
T2  - Food Research International
T1  - Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.
VL  - 170
DO  - 10.1016/j.foodres.2023.113044
SP  - 113044
ER  - 

@article{
author = "De Oliveira, Izamara and Chrysargyris, Antonios and Heleno, Sandrina A. and Carocho, Marcio and Calhelha, Ricardo and Dias, Maria Ines and Petrović, Jovana and Soković, Marina and Petropoulos, Spyridon and Santos-Buelga, Celestino and Tzortzakis, Nikolaos and Barros, Lillian",
year = "2023",
abstract = "The present study aims to determine the combined effect of cropping system and irrigation regime on the chemical composition and bioactive properties of lemon balm aerial parts. For this purpose, lemon balm plants were grown under two farming systems (conventional farming vs organic farming) and two irrigation levels (full irrigation vs deficit irrigation) and harvested twice throughout the growing period. The collected aerial parts were subjected to three different methods of extractions, namely infusion, maceration and ultrasound-assisted extraction and the obtained extracts were evaluated in terms of chemical profile and bioactivities. Five organic acids with varied composition among the tested treatments were identified in all the tested samples for both harvests, namely, citric, malic, oxalic, shikimic and quinic acid. Regarding phenolic compounds composition, the most abundant ones were rosmarinic acid, lithospermic acid A isomer I and hydroxylsalvianolic E, especially for the maceration and infusion extraction methods. Full irrigation resulted in lower EC50 values than deficit irrigation only in the treatments of the second harvest, while variable cytotoxic and anti-inflammatory effects were recorded in both harvests. Finally, in most cases the lemon balm extracts has similar or better activity than the positive controls, while the antifungal activity of lemon balm extracts was stronger than the antibacterial effects. In conclusion, the results of the present study showed that the implemented agronomic practices, as well as the extraction protocol may significantly affect the chemical profile and bioactivities of lemon balm extracts, suggesting that both the farming system and the irrigation schedule may improve the quality of the extracts depending on the implemented extraction protocol.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.",
volume = "170",
doi = "10.1016/j.foodres.2023.113044",
pages = "113044"
}

De Oliveira, I., Chrysargyris, A., Heleno, S. A., Carocho, M., Calhelha, R., Dias, M. I., Petrović, J., Soković, M., Petropoulos, S., Santos-Buelga, C., Tzortzakis, N.,& Barros, L.. (2023). Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.. in Food Research International
Elsevier., 170, 113044.
https://doi.org/10.1016/j.foodres.2023.113044

De Oliveira I, Chrysargyris A, Heleno SA, Carocho M, Calhelha R, Dias MI, Petrović J, Soković M, Petropoulos S, Santos-Buelga C, Tzortzakis N, Barros L. Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.. in Food Research International. 2023;170:113044.
doi:10.1016/j.foodres.2023.113044 .

De Oliveira, Izamara, Chrysargyris, Antonios, Heleno, Sandrina A., Carocho, Marcio, Calhelha, Ricardo, Dias, Maria Ines, Petrović, Jovana, Soković, Marina, Petropoulos, Spyridon, Santos-Buelga, Celestino, Tzortzakis, Nikolaos, Barros, Lillian, "Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes." in Food Research International, 170 (2023):113044,
https://doi.org/10.1016/j.foodres.2023.113044 . .

TY  - JOUR
AU  - Fernandes, Angela
AU  - Polyzos, Nikolaos
AU  - Mandim, Filipa
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Petropoulos, Spyridon
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6399
AB  - The aim of the present study was to evaluate the possible effects of two different bios timulant formulations at different application regimes and combined or not with mineral fertilizers
(e.g., W1–W8, including the control treatment (no formulations added)) on the yield parameters and
fruit quality of watermelon plants. The highest yield was recorded for the W5 treatment due to the
formation of more fruit. The highest content of fat, proteins and ash was recorded for treatment
W1, whereas carbohydrates were the most abundant in the control treatment, resulting also in the
highest energetic value. The main detected sugars in all the tested samples were sucrose and fructose,
which were the highest for the W4 and W5 treatments (sucrose) and W4 treatment (fructose). Malic
and citric acid were the most abundant compounds, especially in the W4 treatment. In terms of
tocopherols, only α-tocopherol was detected, with the highest amounts being recorded for the W4
treatment. Regarding bioactive properties, the lowest IC50 values for OxHLIA were recorded for
the W2, W3 and W8 formulations. Moreover, all the extracts exhibited significant anti-inflammatory
activity comparable to the positive control, while a variable efficacy of the tested extracts against
the studied bacteria and fungi was recorded. In conclusion, our results indicate that simple agro nomic practices such as biostimulant application may improve crop performance and improve the
proximal composition and the overall quality of watermelon fruit within the context of sustainable
crop production.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants
IS  - 7
VL  - 9
DO  - 10.3390/horticulturae9070838
SP  - 838
ER  - 

@article{
author = "Fernandes, Angela and Polyzos, Nikolaos and Mandim, Filipa and Pereira, Carla and Petrović, Jovana and Soković, Marina and Petropoulos, Spyridon",
year = "2023",
abstract = "The aim of the present study was to evaluate the possible effects of two different bios timulant formulations at different application regimes and combined or not with mineral fertilizers
(e.g., W1–W8, including the control treatment (no formulations added)) on the yield parameters and
fruit quality of watermelon plants. The highest yield was recorded for the W5 treatment due to the
formation of more fruit. The highest content of fat, proteins and ash was recorded for treatment
W1, whereas carbohydrates were the most abundant in the control treatment, resulting also in the
highest energetic value. The main detected sugars in all the tested samples were sucrose and fructose,
which were the highest for the W4 and W5 treatments (sucrose) and W4 treatment (fructose). Malic
and citric acid were the most abundant compounds, especially in the W4 treatment. In terms of
tocopherols, only α-tocopherol was detected, with the highest amounts being recorded for the W4
treatment. Regarding bioactive properties, the lowest IC50 values for OxHLIA were recorded for
the W2, W3 and W8 formulations. Moreover, all the extracts exhibited significant anti-inflammatory
activity comparable to the positive control, while a variable efficacy of the tested extracts against
the studied bacteria and fungi was recorded. In conclusion, our results indicate that simple agro nomic practices such as biostimulant application may improve crop performance and improve the
proximal composition and the overall quality of watermelon fruit within the context of sustainable
crop production.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants",
number = "7",
volume = "9",
doi = "10.3390/horticulturae9070838",
pages = "838"
}

Fernandes, A., Polyzos, N., Mandim, F., Pereira, C., Petrović, J., Soković, M.,& Petropoulos, S.. (2023). Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants. in Horticulturae
Basel: MDPI., 9(7), 838.
https://doi.org/10.3390/horticulturae9070838

Fernandes A, Polyzos N, Mandim F, Pereira C, Petrović J, Soković M, Petropoulos S. Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants. in Horticulturae. 2023;9(7):838.
doi:10.3390/horticulturae9070838 .

Fernandes, Angela, Polyzos, Nikolaos, Mandim, Filipa, Pereira, Carla, Petrović, Jovana, Soković, Marina, Petropoulos, Spyridon, "Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants" in Horticulturae, 9, no. 7 (2023):838,
https://doi.org/10.3390/horticulturae9070838 . .

TY  - CONF
AU  - Sampaio, Shirley
AU  - Lonchamp, Julien
AU  - Dias, Maria Inês
AU  - Liddle, Catriona
AU  - Petropoulos, Spyridon
AU  - Glamočlija, Jasmina
AU  - Alexopoulos, Alexios
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel
AU  - Barros, Lillian
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4308
UR  - http://hdl.handle.net/10198/22068
AB  - Aqueous extracts from seven coloured potato varieties (three red-fleshed, three-purple fleshed, and one
marble-fleshed genotype) were studied for their anthocyanin content, in vitro biological activities,
colouring properties and their potential application in the food industry. Acylated glycosides or
pelargonidin and petunidin aglycones were identified as the main anthocyanin forms in the red and purple
varieties, respectively. The total anthocyanin content among varieties ranged from 478.3 to 886.2 mg/100
g extract. All the extracts presented in vitro antioxidant, antibacterial and antifungal activities, whereas no
toxic effects were detected. Finally, two selected extracts were tested as colourants in a soft drink
formulation and presented suitable sensory profiles as well as high colour stability during a 30-day
shelf-life when compared with the commercial colourant E163. Therefore, the tested extracts could be
used as natural food colourants.
PB  - Instituto Politécnico de Bragança, Centro de Investigacao de Montanha
C3  - Book of abstracts, 1st Natural products application: health, cosmetic and food
T1  - Anthocyanin-rich extracts from purple and red potatoes as natural colourants: bioactive properties, application in a soft drink formulation and sensory analysis
SP  - 239
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4308
ER  - 

@conference{
author = "Sampaio, Shirley and Lonchamp, Julien and Dias, Maria Inês and Liddle, Catriona and Petropoulos, Spyridon and Glamočlija, Jasmina and Alexopoulos, Alexios and Santos-Buelga, Celestino and Ferreira, Isabel and Barros, Lillian",
year = "2021",
abstract = "Aqueous extracts from seven coloured potato varieties (three red-fleshed, three-purple fleshed, and one
marble-fleshed genotype) were studied for their anthocyanin content, in vitro biological activities,
colouring properties and their potential application in the food industry. Acylated glycosides or
pelargonidin and petunidin aglycones were identified as the main anthocyanin forms in the red and purple
varieties, respectively. The total anthocyanin content among varieties ranged from 478.3 to 886.2 mg/100
g extract. All the extracts presented in vitro antioxidant, antibacterial and antifungal activities, whereas no
toxic effects were detected. Finally, two selected extracts were tested as colourants in a soft drink
formulation and presented suitable sensory profiles as well as high colour stability during a 30-day
shelf-life when compared with the commercial colourant E163. Therefore, the tested extracts could be
used as natural food colourants.",
publisher = "Instituto Politécnico de Bragança, Centro de Investigacao de Montanha",
journal = "Book of abstracts, 1st Natural products application: health, cosmetic and food",
title = "Anthocyanin-rich extracts from purple and red potatoes as natural colourants: bioactive properties, application in a soft drink formulation and sensory analysis",
pages = "239",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4308"
}

Sampaio, S., Lonchamp, J., Dias, M. I., Liddle, C., Petropoulos, S., Glamočlija, J., Alexopoulos, A., Santos-Buelga, C., Ferreira, I.,& Barros, L.. (2021). Anthocyanin-rich extracts from purple and red potatoes as natural colourants: bioactive properties, application in a soft drink formulation and sensory analysis. in Book of abstracts, 1st Natural products application: health, cosmetic and food
Instituto Politécnico de Bragança, Centro de Investigacao de Montanha., 239.
https://hdl.handle.net/21.15107/rcub_ibiss_4308

Sampaio S, Lonchamp J, Dias MI, Liddle C, Petropoulos S, Glamočlija J, Alexopoulos A, Santos-Buelga C, Ferreira I, Barros L. Anthocyanin-rich extracts from purple and red potatoes as natural colourants: bioactive properties, application in a soft drink formulation and sensory analysis. in Book of abstracts, 1st Natural products application: health, cosmetic and food. 2021;:239.
https://hdl.handle.net/21.15107/rcub_ibiss_4308 .

Sampaio, Shirley, Lonchamp, Julien, Dias, Maria Inês, Liddle, Catriona, Petropoulos, Spyridon, Glamočlija, Jasmina, Alexopoulos, Alexios, Santos-Buelga, Celestino, Ferreira, Isabel, Barros, Lillian, "Anthocyanin-rich extracts from purple and red potatoes as natural colourants: bioactive properties, application in a soft drink formulation and sensory analysis" in Book of abstracts, 1st Natural products application: health, cosmetic and food (2021):239,
https://hdl.handle.net/21.15107/rcub_ibiss_4308 .

TY  - JOUR
AU  - Liberal, Ângela
AU  - Ângela, Fernandes
AU  - Polyzos, Nikolaos
AU  - Petropoulos, Spyridon
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4056
AB  - Petroselinum  crispum  Mill.,  Fuss.,  is  a  culinary  vegetable  used  as  an  aromatic  herb  that  garnishes  and  flavours  a  great  variety  of  dishes.  In  the  present  study,  the  chemical  profiles  and  bioactivities  of  leaf  samples  from  25  cultivars  (three  types:  plain-  and  curly-leafed  and  turnip-rooted)  from  this  species  were  assessed.  Seven  phenolic  compounds  were  identified  in  all  the  varieties, including apigenin and kaempherol derivates. Apigenin-O-pentoside-O-hexoside was the major  compound  in  all  the  tested  parsley  types  (20,  22  and  13  mg/g  of  extract,  respectively)  and  responsible  for  its  excellent  antioxidant  activity,  also  investigated  in  this  study.  Antimicrobial  activities  were  also  explored,  and  the  results  revealed  a  good  bioactivity  against  specific  tested  pathogens, such as bacteria and fungi. In conclusion, the leaves of all the types of P. crispum are a good source of natural bioactive compounds that confer health benefits, and thus, they should be part of a balanced and diversified diet.
PB  - Basel: MDPI
T2  - Molecules (Basel, Switzerland)
T1  - Bioactive Properties and Phenolic Compound Profiles of Turnip-Rooted, Plain-Leafed and Curly-Leafed Parsley Cultivars
IS  - 23
VL  - 25
DO  - 10.3390/molecules25235606
SP  - 5606
ER  - 

@article{
author = "Liberal, Ângela and Ângela, Fernandes and Polyzos, Nikolaos and Petropoulos, Spyridon and Dias, Maria Inês and Pinela, José and Petrović, Jovana and Soković, Marina and Ferreira, Isabel C.F.R. and Barros, Lillian",
year = "2020",
abstract = "Petroselinum  crispum  Mill.,  Fuss.,  is  a  culinary  vegetable  used  as  an  aromatic  herb  that  garnishes  and  flavours  a  great  variety  of  dishes.  In  the  present  study,  the  chemical  profiles  and  bioactivities  of  leaf  samples  from  25  cultivars  (three  types:  plain-  and  curly-leafed  and  turnip-rooted)  from  this  species  were  assessed.  Seven  phenolic  compounds  were  identified  in  all  the  varieties, including apigenin and kaempherol derivates. Apigenin-O-pentoside-O-hexoside was the major  compound  in  all  the  tested  parsley  types  (20,  22  and  13  mg/g  of  extract,  respectively)  and  responsible  for  its  excellent  antioxidant  activity,  also  investigated  in  this  study.  Antimicrobial  activities  were  also  explored,  and  the  results  revealed  a  good  bioactivity  against  specific  tested  pathogens, such as bacteria and fungi. In conclusion, the leaves of all the types of P. crispum are a good source of natural bioactive compounds that confer health benefits, and thus, they should be part of a balanced and diversified diet.",
publisher = "Basel: MDPI",
journal = "Molecules (Basel, Switzerland)",
title = "Bioactive Properties and Phenolic Compound Profiles of Turnip-Rooted, Plain-Leafed and Curly-Leafed Parsley Cultivars",
number = "23",
volume = "25",
doi = "10.3390/molecules25235606",
pages = "5606"
}

Liberal, Â., Ângela, F., Polyzos, N., Petropoulos, S., Dias, M. I., Pinela, J., Petrović, J., Soković, M., Ferreira, I. C.F.R.,& Barros, L.. (2020). Bioactive Properties and Phenolic Compound Profiles of Turnip-Rooted, Plain-Leafed and Curly-Leafed Parsley Cultivars. in Molecules (Basel, Switzerland)
Basel: MDPI., 25(23), 5606.
https://doi.org/10.3390/molecules25235606

Liberal Â, Ângela F, Polyzos N, Petropoulos S, Dias MI, Pinela J, Petrović J, Soković M, Ferreira IC, Barros L. Bioactive Properties and Phenolic Compound Profiles of Turnip-Rooted, Plain-Leafed and Curly-Leafed Parsley Cultivars. in Molecules (Basel, Switzerland). 2020;25(23):5606.
doi:10.3390/molecules25235606 .

Liberal, Ângela, Ângela, Fernandes, Polyzos, Nikolaos, Petropoulos, Spyridon, Dias, Maria Inês, Pinela, José, Petrović, Jovana, Soković, Marina, Ferreira, Isabel C.F.R., Barros, Lillian, "Bioactive Properties and Phenolic Compound Profiles of Turnip-Rooted, Plain-Leafed and Curly-Leafed Parsley Cultivars" in Molecules (Basel, Switzerland), 25, no. 23 (2020):5606,
https://doi.org/10.3390/molecules25235606 . .

TY  - JOUR
AU  - Petropoulos, Spyridon
AU  - Fernandes, Ângela
AU  - Stojković, Dejan
AU  - Pereira, Carla
AU  - Taofiq, Oludemi
AU  - Di Gioia, Francesco
AU  - Tzortzakis, Nikos
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0959652619331245?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3471
AB  - The intensification of horticultural crops cultivation makes urgent the seeking for alternative growth substrates that could substitute non-renewable and/or synthetic growing media, such as peat and rock wool. The aim of the present study was to evaluate the potential use of byproducts from two industrial crops commonly cultivated in the Mediterranean basin, namely cardoon and cotton, as growth substrates for Cichorium spinosum, while zeolite addition was also tested as a soil amendment. A pot experiment was carried for two consecutive growing periods and plant growth was evaluated for six growing media compositions, while plant extracts were also evaluated in terms of their phenolic compounds profile, antioxidant and antimicrobial activities. The results of this study showed that cotton byproducts and zeolite may partially substitute peat in growth substrate of C. spinosum and high yields comparable to peat may be achieved. Phenolic compounds content and antioxidant activity of leaves' extracts was higher for plants grown in soil which showed severe stress symptoms comparing to the other tested substrate blends. Antimicrobial activity was also affected by growth substrate composition, only in the case of antibacterial properties of leaves' extracts, whereas none of the extracts presented significant antifungal activities. In conclusion, the use of cotton ginning byproducts and zeolite in growth substrate blends may partially substitute conventional substrates as peat in horticultural crops production, resulting in reduction of production cost and lessening of bulky byproducts’ management and related environmental burden without compromising yield.
PB  - Elsevier
T2  - Journal of Cleaner Production
T1  - Cotton and cardoon byproducts as potential growing media components for Cichorium spinosum L. commercial cultivation
VL  - 240
DO  - 10.1016/J.JCLEPRO.2019.118254
SP  - 118254
ER  - 

@article{
author = "Petropoulos, Spyridon and Fernandes, Ângela and Stojković, Dejan and Pereira, Carla and Taofiq, Oludemi and Di Gioia, Francesco and Tzortzakis, Nikos and Soković, Marina and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2019",
abstract = "The intensification of horticultural crops cultivation makes urgent the seeking for alternative growth substrates that could substitute non-renewable and/or synthetic growing media, such as peat and rock wool. The aim of the present study was to evaluate the potential use of byproducts from two industrial crops commonly cultivated in the Mediterranean basin, namely cardoon and cotton, as growth substrates for Cichorium spinosum, while zeolite addition was also tested as a soil amendment. A pot experiment was carried for two consecutive growing periods and plant growth was evaluated for six growing media compositions, while plant extracts were also evaluated in terms of their phenolic compounds profile, antioxidant and antimicrobial activities. The results of this study showed that cotton byproducts and zeolite may partially substitute peat in growth substrate of C. spinosum and high yields comparable to peat may be achieved. Phenolic compounds content and antioxidant activity of leaves' extracts was higher for plants grown in soil which showed severe stress symptoms comparing to the other tested substrate blends. Antimicrobial activity was also affected by growth substrate composition, only in the case of antibacterial properties of leaves' extracts, whereas none of the extracts presented significant antifungal activities. In conclusion, the use of cotton ginning byproducts and zeolite in growth substrate blends may partially substitute conventional substrates as peat in horticultural crops production, resulting in reduction of production cost and lessening of bulky byproducts’ management and related environmental burden without compromising yield.",
publisher = "Elsevier",
journal = "Journal of Cleaner Production",
title = "Cotton and cardoon byproducts as potential growing media components for Cichorium spinosum L. commercial cultivation",
volume = "240",
doi = "10.1016/J.JCLEPRO.2019.118254",
pages = "118254"
}

Petropoulos, S., Fernandes, Â., Stojković, D., Pereira, C., Taofiq, O., Di Gioia, F., Tzortzakis, N., Soković, M., Barros, L.,& Ferreira, I. C.F.R.. (2019). Cotton and cardoon byproducts as potential growing media components for Cichorium spinosum L. commercial cultivation. in Journal of Cleaner Production
Elsevier., 240, 118254.
https://doi.org/10.1016/J.JCLEPRO.2019.118254

Petropoulos S, Fernandes Â, Stojković D, Pereira C, Taofiq O, Di Gioia F, Tzortzakis N, Soković M, Barros L, Ferreira IC. Cotton and cardoon byproducts as potential growing media components for Cichorium spinosum L. commercial cultivation. in Journal of Cleaner Production. 2019;240:118254.
doi:10.1016/J.JCLEPRO.2019.118254 .

Petropoulos, Spyridon, Fernandes, Ângela, Stojković, Dejan, Pereira, Carla, Taofiq, Oludemi, Di Gioia, Francesco, Tzortzakis, Nikos, Soković, Marina, Barros, Lillian, Ferreira, Isabel C.F.R., "Cotton and cardoon byproducts as potential growing media components for Cichorium spinosum L. commercial cultivation" in Journal of Cleaner Production, 240 (2019):118254,
https://doi.org/10.1016/J.JCLEPRO.2019.118254 . .

TY  - JOUR
AU  - Petropoulos, Spyridon
AU  - Fernandes, Ângela
AU  - Pereira, Carla
AU  - Tzortzakis, Nikos
AU  - Vaz, Josiana
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0308814619305436?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3314
AB  - In the present study, the nutritional value, bioactive properties, and chemical composition of various cardoon (Cynara cardunculus L.) genotypes cultivated in central Greece were investigated. The results demonstrated that Cynara seeds are a good source of fat and protein, while they also contain considerable amounts of K, Mg, and Fe and low amount of Na. Sucrose, oxalic acid, and α-tocopherol were the only free sugar, organic acid, and tocopherol isoform respectively, found among the studied genotypes. The most abundant fatty acids were linoleic, oleic and palmitic acid, while PUFA was the most abundant fatty acid class. All the tested seeds contained only two phenolic compounds, namely 5-O-caffeoylquinic acid and 3,5-O-caffeoylquinic acid, while significant antioxidant activities and cytotoxicity against tumor cell lines and antimicrobial effects were also observed. In conclusion, cardoon seed extracts could be exploited in the food and pharmaceutical industries as alternative sources of natural compounds with bioactive properties.
T2  - Food Chemistry
T1  - Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds
VL  - 289
DO  - 10.1016/J.FOODCHEM.2019.03.066
SP  - 404
EP  - 412
ER  - 

@article{
author = "Petropoulos, Spyridon and Fernandes, Ângela and Pereira, Carla and Tzortzakis, Nikos and Vaz, Josiana and Soković, Marina and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2019",
abstract = "In the present study, the nutritional value, bioactive properties, and chemical composition of various cardoon (Cynara cardunculus L.) genotypes cultivated in central Greece were investigated. The results demonstrated that Cynara seeds are a good source of fat and protein, while they also contain considerable amounts of K, Mg, and Fe and low amount of Na. Sucrose, oxalic acid, and α-tocopherol were the only free sugar, organic acid, and tocopherol isoform respectively, found among the studied genotypes. The most abundant fatty acids were linoleic, oleic and palmitic acid, while PUFA was the most abundant fatty acid class. All the tested seeds contained only two phenolic compounds, namely 5-O-caffeoylquinic acid and 3,5-O-caffeoylquinic acid, while significant antioxidant activities and cytotoxicity against tumor cell lines and antimicrobial effects were also observed. In conclusion, cardoon seed extracts could be exploited in the food and pharmaceutical industries as alternative sources of natural compounds with bioactive properties.",
journal = "Food Chemistry",
title = "Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds",
volume = "289",
doi = "10.1016/J.FOODCHEM.2019.03.066",
pages = "404-412"
}

Petropoulos, S., Fernandes, Â., Pereira, C., Tzortzakis, N., Vaz, J., Soković, M., Barros, L.,& Ferreira, I. C.F.R.. (2019). Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds. in Food Chemistry, 289, 404-412.
https://doi.org/10.1016/J.FOODCHEM.2019.03.066

Petropoulos S, Fernandes Â, Pereira C, Tzortzakis N, Vaz J, Soković M, Barros L, Ferreira IC. Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds. in Food Chemistry. 2019;289:404-412.
doi:10.1016/J.FOODCHEM.2019.03.066 .

Petropoulos, Spyridon, Fernandes, Ângela, Pereira, Carla, Tzortzakis, Nikos, Vaz, Josiana, Soković, Marina, Barros, Lillian, Ferreira, Isabel C.F.R., "Bioactivities, chemical composition and nutritional value of Cynara cardunculus L. seeds" in Food Chemistry, 289 (2019):404-412,
https://doi.org/10.1016/J.FOODCHEM.2019.03.066 . .

TY  - JOUR
AU  - Karkanis, Anestis C.
AU  - Fernandes, Ângela
AU  - Vaz, Josiana
AU  - Petropoulos, Spyridon
AU  - Georgiou, Eleftheria
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Oludemi, Taofiq
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2019
UR  - http://xlink.rsc.org/?DOI=C8FO02601G
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3311
AB  - Sanguisorba minor Scop. is a perennial plant native to the Mediterranean region which exhibits several medicinal activities. In the present study, plant growth, chemical composition, and antitumor and antimicrobial properties of S. minor plants were evaluated under different growing conditions. In particular, plants were grown on different substrates, namely (A) peat, (B) peat : perlite (1 : 1) and (C) peat : perlite (2 : 1). The dry weight of the aerial parts and roots was higher for the peat treatment (A), whereas plant growth was severely affected in the peat : perlite (1 : 1) treatment (B). The major detected sugars were fructose and glucose, while the peat : perlite (2 : 1) treatment resulted in a significant increase of α-tocopherol, glucose and total sugars content, especially in the aerial parts. Oxalic acid and citric acid were the major organic acids detected, with a varied effect of growth substrate on organic acid profile. Growth substrate and plant part also had a significant effect on fatty acid composition, especially on linoleic acid and α-linolenic acid content which was higher in the peat : perlite (1 : 1) treatment in both aerial parts and roots. An impact on the composition of phenolic compounds was also observed, where the plants grown on peat : perlite (2 : 1) and peat : perlite (1 : 1) substrates had the highest content of individual and total phenolic compounds in their aerial parts and roots, respectively. Moreover, roots contained significantly higher amounts of phenolic compounds than the aerial parts. Moreover, plant extracts exhibited antiproliferative activity against four tumor cell lines (HeLa, HepG2, MCF-7 and NCI-H460) and a primary culture of porcine liver cells (PLP2), as well as significant antimicrobial properties. In conclusion, S. minor presented significant bioactive properties, while growth substrates affected the nutritional value, chemical composition, antitumor and antimicrobial properties of the species which could be probably attributed to the higher phenolic compound content and different compositions of phenolic compounds. Therefore, although the species is commonly found in the Mediterranean region, it is underexploited yet and its plant tissues could be a potential source of natural bioactive compounds with further use in pharmaceutical and medicinal applications.
T2  - Food & Function
T1  - Chemical composition and bioactive properties of Sanguisorba minor Scop. under Mediterranean growing conditions.
IS  - 3
VL  - 10
DO  - 10.1039/c8fo02601g
SP  - 1340
EP  - 1351
ER  - 

@article{
author = "Karkanis, Anestis C. and Fernandes, Ângela and Vaz, Josiana and Petropoulos, Spyridon and Georgiou, Eleftheria and Ćirić, Ana and Soković, Marina and Oludemi, Taofiq and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2019",
abstract = "Sanguisorba minor Scop. is a perennial plant native to the Mediterranean region which exhibits several medicinal activities. In the present study, plant growth, chemical composition, and antitumor and antimicrobial properties of S. minor plants were evaluated under different growing conditions. In particular, plants were grown on different substrates, namely (A) peat, (B) peat : perlite (1 : 1) and (C) peat : perlite (2 : 1). The dry weight of the aerial parts and roots was higher for the peat treatment (A), whereas plant growth was severely affected in the peat : perlite (1 : 1) treatment (B). The major detected sugars were fructose and glucose, while the peat : perlite (2 : 1) treatment resulted in a significant increase of α-tocopherol, glucose and total sugars content, especially in the aerial parts. Oxalic acid and citric acid were the major organic acids detected, with a varied effect of growth substrate on organic acid profile. Growth substrate and plant part also had a significant effect on fatty acid composition, especially on linoleic acid and α-linolenic acid content which was higher in the peat : perlite (1 : 1) treatment in both aerial parts and roots. An impact on the composition of phenolic compounds was also observed, where the plants grown on peat : perlite (2 : 1) and peat : perlite (1 : 1) substrates had the highest content of individual and total phenolic compounds in their aerial parts and roots, respectively. Moreover, roots contained significantly higher amounts of phenolic compounds than the aerial parts. Moreover, plant extracts exhibited antiproliferative activity against four tumor cell lines (HeLa, HepG2, MCF-7 and NCI-H460) and a primary culture of porcine liver cells (PLP2), as well as significant antimicrobial properties. In conclusion, S. minor presented significant bioactive properties, while growth substrates affected the nutritional value, chemical composition, antitumor and antimicrobial properties of the species which could be probably attributed to the higher phenolic compound content and different compositions of phenolic compounds. Therefore, although the species is commonly found in the Mediterranean region, it is underexploited yet and its plant tissues could be a potential source of natural bioactive compounds with further use in pharmaceutical and medicinal applications.",
journal = "Food & Function",
title = "Chemical composition and bioactive properties of Sanguisorba minor Scop. under Mediterranean growing conditions.",
number = "3",
volume = "10",
doi = "10.1039/c8fo02601g",
pages = "1340-1351"
}

Karkanis, A. C., Fernandes, Â., Vaz, J., Petropoulos, S., Georgiou, E., Ćirić, A., Soković, M., Oludemi, T., Barros, L.,& Ferreira, I. C. F. R.. (2019). Chemical composition and bioactive properties of Sanguisorba minor Scop. under Mediterranean growing conditions.. in Food & Function, 10(3), 1340-1351.
https://doi.org/10.1039/c8fo02601g

Karkanis AC, Fernandes Â, Vaz J, Petropoulos S, Georgiou E, Ćirić A, Soković M, Oludemi T, Barros L, Ferreira ICFR. Chemical composition and bioactive properties of Sanguisorba minor Scop. under Mediterranean growing conditions.. in Food & Function. 2019;10(3):1340-1351.
doi:10.1039/c8fo02601g .

Karkanis, Anestis C., Fernandes, Ângela, Vaz, Josiana, Petropoulos, Spyridon, Georgiou, Eleftheria, Ćirić, Ana, Soković, Marina, Oludemi, Taofiq, Barros, Lillian, Ferreira, Isabel C. F. R., "Chemical composition and bioactive properties of Sanguisorba minor Scop. under Mediterranean growing conditions." in Food & Function, 10, no. 3 (2019):1340-1351,
https://doi.org/10.1039/c8fo02601g . .

TY  - CONF
AU  - Fernandes, Ângela
AU  - Petropoulos, Spyridon
AU  - Barros, Lillian
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4364
AB  - Garlic (Allium sativum L.) is one of the most economically important species of the Allium genus throughout the 
world. One of the major beneficial effects of garlic is related with antioxidant properties which have been associated 
with many therapeutic effects, including cancer prevention, antithrombotic effects, cardiovascular protection and anti aging effects. 
The aim of the present study was to determine the bioactive properties from various Greek garlics in order to determine 
the variability in their antimicrobial and antioxidant properties, as well as to compare them with commercial cultivars. 
The collected samples included: one local garlic cultivar from Evros “Nea Vissa” (samples G1 and G2); one sample 
of a local landrace and one sample of Chinese origin cultivated in Neapoli; (samples G3 and G4, respectively); one 
sample from Euboea Prefecture of Chinese origin (G5); one sample of local cultivar of “Platykampos”, one 
commercial sample of Chinese origin and one sample of commercial cultivar Gardos, all cultivated in Magnissia 
Perfecture (G6-G8, respectively), and three samples from Arcadia Prefecture of local cultivar of “Tripoli” (G9-11). 
The antioxidant activity was evaluated by DPPH radical-scavenging activity, reducing power, inhibition of beta carotene bleaching and inhibition of lipid peroxidation. The antimicrobial activity was evaluated against four Gram 
positive bacteria, six Gram negative bacteria and two fungi, using the microdilution method, in order to obtain the 
minimal growth inhibititory concentrations and minimal bactericidal/funcicidal concentrations.
Genotype G5 showed the lowest EC50 values for all the tested antioxidant activity assays. The antimicrobial activity 
was significant, especially against the bacteria P. mirabilis and antibiotic resistant E. coli. Significant variation was 
observed between the studied garlic genotypes, indicating the importance of both growing conditions and genotype 
on the bioactive properties of dry garlic. This variation could be further exploited in breeding programs in order to 
select elite genotypes with increased bioactive properties.
PB  - Belgrade: University of Belgrade
C3  - UNIFood Conference; 2018 Oct 5-6
T1  - Biactive properties of Greek garlic genotypes
SP  - 234
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4364
ER  - 

@conference{
author = "Fernandes, Ângela and Petropoulos, Spyridon and Barros, Lillian and Ćirić, Ana and Soković, Marina and Ferreira, Isabel C. F. R.",
year = "2018",
abstract = "Garlic (Allium sativum L.) is one of the most economically important species of the Allium genus throughout the 
world. One of the major beneficial effects of garlic is related with antioxidant properties which have been associated 
with many therapeutic effects, including cancer prevention, antithrombotic effects, cardiovascular protection and anti aging effects. 
The aim of the present study was to determine the bioactive properties from various Greek garlics in order to determine 
the variability in their antimicrobial and antioxidant properties, as well as to compare them with commercial cultivars. 
The collected samples included: one local garlic cultivar from Evros “Nea Vissa” (samples G1 and G2); one sample 
of a local landrace and one sample of Chinese origin cultivated in Neapoli; (samples G3 and G4, respectively); one 
sample from Euboea Prefecture of Chinese origin (G5); one sample of local cultivar of “Platykampos”, one 
commercial sample of Chinese origin and one sample of commercial cultivar Gardos, all cultivated in Magnissia 
Perfecture (G6-G8, respectively), and three samples from Arcadia Prefecture of local cultivar of “Tripoli” (G9-11). 
The antioxidant activity was evaluated by DPPH radical-scavenging activity, reducing power, inhibition of beta carotene bleaching and inhibition of lipid peroxidation. The antimicrobial activity was evaluated against four Gram 
positive bacteria, six Gram negative bacteria and two fungi, using the microdilution method, in order to obtain the 
minimal growth inhibititory concentrations and minimal bactericidal/funcicidal concentrations.
Genotype G5 showed the lowest EC50 values for all the tested antioxidant activity assays. The antimicrobial activity 
was significant, especially against the bacteria P. mirabilis and antibiotic resistant E. coli. Significant variation was 
observed between the studied garlic genotypes, indicating the importance of both growing conditions and genotype 
on the bioactive properties of dry garlic. This variation could be further exploited in breeding programs in order to 
select elite genotypes with increased bioactive properties.",
publisher = "Belgrade: University of Belgrade",
journal = "UNIFood Conference; 2018 Oct 5-6",
title = "Biactive properties of Greek garlic genotypes",
pages = "234",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4364"
}

Fernandes, Â., Petropoulos, S., Barros, L., Ćirić, A., Soković, M.,& Ferreira, I. C. F. R.. (2018). Biactive properties of Greek garlic genotypes. in UNIFood Conference; 2018 Oct 5-6
Belgrade: University of Belgrade., 234.
https://hdl.handle.net/21.15107/rcub_ibiss_4364

Fernandes Â, Petropoulos S, Barros L, Ćirić A, Soković M, Ferreira ICFR. Biactive properties of Greek garlic genotypes. in UNIFood Conference; 2018 Oct 5-6. 2018;:234.
https://hdl.handle.net/21.15107/rcub_ibiss_4364 .

Fernandes, Ângela, Petropoulos, Spyridon, Barros, Lillian, Ćirić, Ana, Soković, Marina, Ferreira, Isabel C. F. R., "Biactive properties of Greek garlic genotypes" in UNIFood Conference; 2018 Oct 5-6 (2018):234,
https://hdl.handle.net/21.15107/rcub_ibiss_4364 .

TY  - JOUR
AU  - Petropoulos, Spyridon
AU  - Fernandes, Ângela
AU  - Barros, Lillian
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Ferreira, Isabel C.F.R.
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0308814617317168
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2896
AB  - Recent studies show a significant variation in antioxidant and antimicrobial properties between the various garlic genotypes mostly due to differences in chemical composition and bioactive compounds content. The aim of the present study was to evaluate antioxidant properties and antimicrobial activity of garlics collected from the main cultivation areas of Greece, as well as to correlate this activity with their total phenolics content. Genotype G5 showed the highest total phenolics content, which was significantly correlated with the lowest EC50 values for all the tested antioxidant activity assays. Antimicrobial activity was significant, especially against the bacteria Proteus mirabilis and Antibiotic resistant Escherichia coli. In conclusion, significant variation was observed between the studied garlic genotypes, indicating the importance of both growing conditions and genotype on bioactive properties of dry garlic bulbs. This variation could be further exploited in breeding programs in order to select elite genotypes with increased bioactive properties.
T2  - Food Chemistry
T1  - Antimicrobial and antioxidant properties of various Greek garlic genotypes
VL  - 245
DO  - 10.1016/j.foodchem.2017.10.078
SP  - 7
EP  - 12
ER  - 

@article{
author = "Petropoulos, Spyridon and Fernandes, Ângela and Barros, Lillian and Ćirić, Ana and Soković, Marina and Ferreira, Isabel C.F.R.",
year = "2018",
abstract = "Recent studies show a significant variation in antioxidant and antimicrobial properties between the various garlic genotypes mostly due to differences in chemical composition and bioactive compounds content. The aim of the present study was to evaluate antioxidant properties and antimicrobial activity of garlics collected from the main cultivation areas of Greece, as well as to correlate this activity with their total phenolics content. Genotype G5 showed the highest total phenolics content, which was significantly correlated with the lowest EC50 values for all the tested antioxidant activity assays. Antimicrobial activity was significant, especially against the bacteria Proteus mirabilis and Antibiotic resistant Escherichia coli. In conclusion, significant variation was observed between the studied garlic genotypes, indicating the importance of both growing conditions and genotype on bioactive properties of dry garlic bulbs. This variation could be further exploited in breeding programs in order to select elite genotypes with increased bioactive properties.",
journal = "Food Chemistry",
title = "Antimicrobial and antioxidant properties of various Greek garlic genotypes",
volume = "245",
doi = "10.1016/j.foodchem.2017.10.078",
pages = "7-12"
}

Petropoulos, S., Fernandes, Â., Barros, L., Ćirić, A., Soković, M.,& Ferreira, I. C.F.R.. (2018). Antimicrobial and antioxidant properties of various Greek garlic genotypes. in Food Chemistry, 245, 7-12.
https://doi.org/10.1016/j.foodchem.2017.10.078

Petropoulos S, Fernandes Â, Barros L, Ćirić A, Soković M, Ferreira IC. Antimicrobial and antioxidant properties of various Greek garlic genotypes. in Food Chemistry. 2018;245:7-12.
doi:10.1016/j.foodchem.2017.10.078 .

Petropoulos, Spyridon, Fernandes, Ângela, Barros, Lillian, Ćirić, Ana, Soković, Marina, Ferreira, Isabel C.F.R., "Antimicrobial and antioxidant properties of various Greek garlic genotypes" in Food Chemistry, 245 (2018):7-12,
https://doi.org/10.1016/j.foodchem.2017.10.078 . .