TY  - JOUR
AU  - Cavichi, Lucas Vinicius
AU  - Liberal, Ângela
AU  - Dias, Maria Inês
AU  - Mandim, Filipa
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Kalschne, Daneysa Lahis
AU  - Fernandes, Ângela
AU  - Canan, Cristiane
AU  - Barros, Lillian
AU  - Amaral, Joana S.
PY  - 2023
UR  - https://www.mdpi.com/2304-8158/12/1/192
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36613411
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9818490
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5400
AB  - In recent years, the interest in products of natural origin has boosted the exploitation and use of plants as food and sources of bioactive compounds, especially wild plants widely used in different cultures for several purposes. Commelina erecta is a wild edible plant (WEP) traditionally used as food and medicine, about which few studies exist. Thus, this study aimed at enhancing the knowledge about its nutritional, chemical and bioactive profile, considering different plant parts and development stages, in order to increase its inclusion in the diet of South American communities. The nutritional profile was found to be similar to other WEP frequently consumed in Brazil. Thirteen phenolic compounds (HPLC-DAD-ESI/MS) were tentatively identified, with apigenin, luteolin and quercetin derivatives being the most abundant. Fructose and oxalic acid were the major sugar and organic acid, respectively, in the aerial parts of C. erecta, and four isoforms of tocopherols were also identified. Regarding the plant's antioxidant activity, the EC50 values varied between 18.4 and 1060 µg/mL in the inhibition of lipid peroxidation assay (TBARS) and between 53 and 115 µg/mL in the oxidative haemolysis inhibition (OxHLIA) assay. The hydroethanolic extract obtained from stems at the flowering stage also presented anti-inflammatory activity. In general, all the extracts evidenced promising antimicrobial activity. Altogether, these results reinforce the traditional use of this plant species as food and medicine to support the diet of needier populations and also promote food sovereignty and sustainability.
PB  - Basel: MDPI
T2  - Foods
T1  - Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil
IS  - 1
VL  - 12
DO  - 10.3390/foods12010192
SP  - 192
ER  - 

@article{
author = "Cavichi, Lucas Vinicius and Liberal, Ângela and Dias, Maria Inês and Mandim, Filipa and Pinela, José and Kostić, Marina and Soković, Marina and Kalschne, Daneysa Lahis and Fernandes, Ângela and Canan, Cristiane and Barros, Lillian and Amaral, Joana S.",
year = "2023",
abstract = "In recent years, the interest in products of natural origin has boosted the exploitation and use of plants as food and sources of bioactive compounds, especially wild plants widely used in different cultures for several purposes. Commelina erecta is a wild edible plant (WEP) traditionally used as food and medicine, about which few studies exist. Thus, this study aimed at enhancing the knowledge about its nutritional, chemical and bioactive profile, considering different plant parts and development stages, in order to increase its inclusion in the diet of South American communities. The nutritional profile was found to be similar to other WEP frequently consumed in Brazil. Thirteen phenolic compounds (HPLC-DAD-ESI/MS) were tentatively identified, with apigenin, luteolin and quercetin derivatives being the most abundant. Fructose and oxalic acid were the major sugar and organic acid, respectively, in the aerial parts of C. erecta, and four isoforms of tocopherols were also identified. Regarding the plant's antioxidant activity, the EC50 values varied between 18.4 and 1060 µg/mL in the inhibition of lipid peroxidation assay (TBARS) and between 53 and 115 µg/mL in the oxidative haemolysis inhibition (OxHLIA) assay. The hydroethanolic extract obtained from stems at the flowering stage also presented anti-inflammatory activity. In general, all the extracts evidenced promising antimicrobial activity. Altogether, these results reinforce the traditional use of this plant species as food and medicine to support the diet of needier populations and also promote food sovereignty and sustainability.",
publisher = "Basel: MDPI",
journal = "Foods",
title = "Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil",
number = "1",
volume = "12",
doi = "10.3390/foods12010192",
pages = "192"
}

Cavichi, L. V., Liberal, Â., Dias, M. I., Mandim, F., Pinela, J., Kostić, M., Soković, M., Kalschne, D. L., Fernandes, Â., Canan, C., Barros, L.,& Amaral, J. S.. (2023). Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil. in Foods
Basel: MDPI., 12(1), 192.
https://doi.org/10.3390/foods12010192

Cavichi LV, Liberal Â, Dias MI, Mandim F, Pinela J, Kostić M, Soković M, Kalschne DL, Fernandes Â, Canan C, Barros L, Amaral JS. Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil. in Foods. 2023;12(1):192.
doi:10.3390/foods12010192 .

Cavichi, Lucas Vinicius, Liberal, Ângela, Dias, Maria Inês, Mandim, Filipa, Pinela, José, Kostić, Marina, Soković, Marina, Kalschne, Daneysa Lahis, Fernandes, Ângela, Canan, Cristiane, Barros, Lillian, Amaral, Joana S., "Chemical Composition and Biological Activity of Commelina erecta: An Edible Wild Plant Consumed in Brazil" in Foods, 12, no. 1 (2023):192,
https://doi.org/10.3390/foods12010192 . .

TY  - JOUR
AU  - Fernandes, Angela
AU  - Polyzos, Nikolaos
AU  - Mandim, Filipa
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Petropoulos, Spyridon
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6399
AB  - The aim of the present study was to evaluate the possible effects of two different bios timulant formulations at different application regimes and combined or not with mineral fertilizers
(e.g., W1–W8, including the control treatment (no formulations added)) on the yield parameters and
fruit quality of watermelon plants. The highest yield was recorded for the W5 treatment due to the
formation of more fruit. The highest content of fat, proteins and ash was recorded for treatment
W1, whereas carbohydrates were the most abundant in the control treatment, resulting also in the
highest energetic value. The main detected sugars in all the tested samples were sucrose and fructose,
which were the highest for the W4 and W5 treatments (sucrose) and W4 treatment (fructose). Malic
and citric acid were the most abundant compounds, especially in the W4 treatment. In terms of
tocopherols, only α-tocopherol was detected, with the highest amounts being recorded for the W4
treatment. Regarding bioactive properties, the lowest IC50 values for OxHLIA were recorded for
the W2, W3 and W8 formulations. Moreover, all the extracts exhibited significant anti-inflammatory
activity comparable to the positive control, while a variable efficacy of the tested extracts against
the studied bacteria and fungi was recorded. In conclusion, our results indicate that simple agro nomic practices such as biostimulant application may improve crop performance and improve the
proximal composition and the overall quality of watermelon fruit within the context of sustainable
crop production.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants
IS  - 7
VL  - 9
DO  - 10.3390/horticulturae9070838
SP  - 838
ER  - 

@article{
author = "Fernandes, Angela and Polyzos, Nikolaos and Mandim, Filipa and Pereira, Carla and Petrović, Jovana and Soković, Marina and Petropoulos, Spyridon",
year = "2023",
abstract = "The aim of the present study was to evaluate the possible effects of two different bios timulant formulations at different application regimes and combined or not with mineral fertilizers
(e.g., W1–W8, including the control treatment (no formulations added)) on the yield parameters and
fruit quality of watermelon plants. The highest yield was recorded for the W5 treatment due to the
formation of more fruit. The highest content of fat, proteins and ash was recorded for treatment
W1, whereas carbohydrates were the most abundant in the control treatment, resulting also in the
highest energetic value. The main detected sugars in all the tested samples were sucrose and fructose,
which were the highest for the W4 and W5 treatments (sucrose) and W4 treatment (fructose). Malic
and citric acid were the most abundant compounds, especially in the W4 treatment. In terms of
tocopherols, only α-tocopherol was detected, with the highest amounts being recorded for the W4
treatment. Regarding bioactive properties, the lowest IC50 values for OxHLIA were recorded for
the W2, W3 and W8 formulations. Moreover, all the extracts exhibited significant anti-inflammatory
activity comparable to the positive control, while a variable efficacy of the tested extracts against
the studied bacteria and fungi was recorded. In conclusion, our results indicate that simple agro nomic practices such as biostimulant application may improve crop performance and improve the
proximal composition and the overall quality of watermelon fruit within the context of sustainable
crop production.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants",
number = "7",
volume = "9",
doi = "10.3390/horticulturae9070838",
pages = "838"
}

Fernandes, A., Polyzos, N., Mandim, F., Pereira, C., Petrović, J., Soković, M.,& Petropoulos, S.. (2023). Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants. in Horticulturae
Basel: MDPI., 9(7), 838.
https://doi.org/10.3390/horticulturae9070838

Fernandes A, Polyzos N, Mandim F, Pereira C, Petrović J, Soković M, Petropoulos S. Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants. in Horticulturae. 2023;9(7):838.
doi:10.3390/horticulturae9070838 .

Fernandes, Angela, Polyzos, Nikolaos, Mandim, Filipa, Pereira, Carla, Petrović, Jovana, Soković, Marina, Petropoulos, Spyridon, "Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants" in Horticulturae, 9, no. 7 (2023):838,
https://doi.org/10.3390/horticulturae9070838 . .

TY  - JOUR
AU  - da Silva, Tamires Barlati Vieira
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Mandim, Filipa
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
AU  - Seixas, Flávio Augusto Vicente
AU  - Bracht, Adelar
AU  - Peralta, Rosane Marina
PY  - 2023
UR  - http://xlink.rsc.org/?DOI=D2FO02442J
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36723015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5465
AB  - A variety of the classic green tea plant, Camellia sinensis, was developed and is exclusive to Kenya. Due to high content of anthocyanin polyphenols in its leaves, the beverage obtained from this variety is purple in color and is the origin of the name purple tea. This work had two main purposes. The first one was to identify and quantify the major anthocyanin polyphenols in a hot water aqueous extract of the purple tea leaves. The second one was to test the hypothesis if this extract is capable of inhibiting triglyceride absorption considering that anthocyanin polyphenolics have been frequently associated to antilipidemic effects. Parallel experiments were always done with a similar green tea extract for comparison purposes. The antioxidant, anti-inflammatory, and cytotoxic activities of both tea varieties are similar. The purple tea extract, however, was strongly inhibitory toward the pancreatic lipase (minimal IC50 = 67.4 μg mL-1), whereas the green tea preparation was a weak inhibitor. Triglyceride digestion in mice was inhibited by the purple tea extract starting at 100 mg kg-1 dose and with a well-defined dose dependence. Green tea had no effect on triglyceride digestion at doses up to 500 mg kg-1. The latter effect is probably caused by several components in the purple tea extract including non-anthocyanin and anthocyanin polyphenols, the first ones acting solely via the inhibition of the pancreatic lipase and the latter by inhibiting both the lipase and the transport of free fatty acids from the intestinal lumen into the circulating blood. The results suggest that the regular consumption of Kenyan purple tea can be useful in the control of obesity.
T2  - Food & Function
T1  - Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice
IS  - 3
VL  - 14
DO  - 10.1039/d2fo02442j
SP  - 1761
EP  - 1772
ER  - 

@article{
author = "da Silva, Tamires Barlati Vieira and Dias, Maria Inês and Pereira, Carla and Mandim, Filipa and Ivanov, Marija and Soković, Marina and Ferreira, Isabel C. F. R. and Barros, Lillian and Seixas, Flávio Augusto Vicente and Bracht, Adelar and Peralta, Rosane Marina",
year = "2023",
abstract = "A variety of the classic green tea plant, Camellia sinensis, was developed and is exclusive to Kenya. Due to high content of anthocyanin polyphenols in its leaves, the beverage obtained from this variety is purple in color and is the origin of the name purple tea. This work had two main purposes. The first one was to identify and quantify the major anthocyanin polyphenols in a hot water aqueous extract of the purple tea leaves. The second one was to test the hypothesis if this extract is capable of inhibiting triglyceride absorption considering that anthocyanin polyphenolics have been frequently associated to antilipidemic effects. Parallel experiments were always done with a similar green tea extract for comparison purposes. The antioxidant, anti-inflammatory, and cytotoxic activities of both tea varieties are similar. The purple tea extract, however, was strongly inhibitory toward the pancreatic lipase (minimal IC50 = 67.4 μg mL-1), whereas the green tea preparation was a weak inhibitor. Triglyceride digestion in mice was inhibited by the purple tea extract starting at 100 mg kg-1 dose and with a well-defined dose dependence. Green tea had no effect on triglyceride digestion at doses up to 500 mg kg-1. The latter effect is probably caused by several components in the purple tea extract including non-anthocyanin and anthocyanin polyphenols, the first ones acting solely via the inhibition of the pancreatic lipase and the latter by inhibiting both the lipase and the transport of free fatty acids from the intestinal lumen into the circulating blood. The results suggest that the regular consumption of Kenyan purple tea can be useful in the control of obesity.",
journal = "Food & Function",
title = "Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice",
number = "3",
volume = "14",
doi = "10.1039/d2fo02442j",
pages = "1761-1772"
}

da Silva, T. B. V., Dias, M. I., Pereira, C., Mandim, F., Ivanov, M., Soković, M., Ferreira, I. C. F. R., Barros, L., Seixas, F. A. V., Bracht, A.,& Peralta, R. M.. (2023). Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice. in Food & Function, 14(3), 1761-1772.
https://doi.org/10.1039/d2fo02442j

da Silva TBV, Dias MI, Pereira C, Mandim F, Ivanov M, Soković M, Ferreira ICFR, Barros L, Seixas FAV, Bracht A, Peralta RM. Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice. in Food & Function. 2023;14(3):1761-1772.
doi:10.1039/d2fo02442j .

da Silva, Tamires Barlati Vieira, Dias, Maria Inês, Pereira, Carla, Mandim, Filipa, Ivanov, Marija, Soković, Marina, Ferreira, Isabel C. F. R., Barros, Lillian, Seixas, Flávio Augusto Vicente, Bracht, Adelar, Peralta, Rosane Marina, "Purple tea: chemical characterization and evaluation as inhibitor of pancreatic lipase and fat digestion in mice" in Food & Function, 14, no. 3 (2023):1761-1772,
https://doi.org/10.1039/d2fo02442j . .

TY  - JOUR
AU  - Besrour, Nermine
AU  - Oludemi, Taofiq
AU  - Mandim, Filipa
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Soković, Marina
AU  - Stojković, Dejan
AU  - Ferreira, Olga
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2022
UR  - https://www.mdpi.com/2076-3921/11/4/677
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9031312
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4963
AB  - The cosmetic industry is constantly searching for bioactive ingredients, namely, those obtained from natural sources with environmentally friendly connotations and less toxic effects. A previous study of our research group optimized the extraction of phenolic compounds from Juglans regia by heat-assisted extraction. Due to its richness in different phenolic compounds, the present work aimed to develop a formulation containing J. regia leaf extract. The extract's antioxidant, anti-tyrosinase, antimicrobial, anti-inflammatory, wound healing, cytotoxicity, and photostability properties were evaluated. The extract was then incorporated into an O/W base cream, followed by characterization of the final formulation in terms of its antioxidant properties, phenolic composition, and stability over time and at different storage conditions. The most abundant compounds in the hydroethanolic extract were 3-O-caffeoylquinic acid (18.30 ± 0.04 mg/g), quercetin-O-pentoside (9.64 ± 0.06 mg/g), and quercetin 3-O-glucoside (6.70 ± 0.19 mg/g). Besides those, the extract presented antioxidant, anti-inflammatory, wound closure, and antibacterial effects against several skin pathogens. In addition, HaCaT cell viability was maintained up to 98% at 400 µg/mL. Within Proteus vulgaris-infected HaCaT cells, the extract also presented an over 40% bacterial mortality rate at its nontoxic concentration (200 µg/mL). After incorporating the extract, the obtained formulation presented a good physicochemical profile over time and at different storage conditions while also maintaining its antioxidant effect; as such, it can be considered stable for topical application. Future work to evaluate its performance in terms of skin permeation and detailed toxicological studies with a focus on regulatory requirements, involving skin irritation, eye irritation, genotoxicity, photo-irritation, and dermal absorption, should be conducted, as the prepared formulation demonstrated relevant properties that deserve to be further explored.
PB  - Basel: MDPI
T2  - Antioxidants (Basel, Switzerland)
T1  - Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development.
IS  - 4
VL  - 11
DO  - 10.3390/antiox11040677
SP  - 677
ER  - 

@article{
author = "Besrour, Nermine and Oludemi, Taofiq and Mandim, Filipa and Pereira, Carla and Dias, Maria Inês and Soković, Marina and Stojković, Dejan and Ferreira, Olga and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2022",
abstract = "The cosmetic industry is constantly searching for bioactive ingredients, namely, those obtained from natural sources with environmentally friendly connotations and less toxic effects. A previous study of our research group optimized the extraction of phenolic compounds from Juglans regia by heat-assisted extraction. Due to its richness in different phenolic compounds, the present work aimed to develop a formulation containing J. regia leaf extract. The extract's antioxidant, anti-tyrosinase, antimicrobial, anti-inflammatory, wound healing, cytotoxicity, and photostability properties were evaluated. The extract was then incorporated into an O/W base cream, followed by characterization of the final formulation in terms of its antioxidant properties, phenolic composition, and stability over time and at different storage conditions. The most abundant compounds in the hydroethanolic extract were 3-O-caffeoylquinic acid (18.30 ± 0.04 mg/g), quercetin-O-pentoside (9.64 ± 0.06 mg/g), and quercetin 3-O-glucoside (6.70 ± 0.19 mg/g). Besides those, the extract presented antioxidant, anti-inflammatory, wound closure, and antibacterial effects against several skin pathogens. In addition, HaCaT cell viability was maintained up to 98% at 400 µg/mL. Within Proteus vulgaris-infected HaCaT cells, the extract also presented an over 40% bacterial mortality rate at its nontoxic concentration (200 µg/mL). After incorporating the extract, the obtained formulation presented a good physicochemical profile over time and at different storage conditions while also maintaining its antioxidant effect; as such, it can be considered stable for topical application. Future work to evaluate its performance in terms of skin permeation and detailed toxicological studies with a focus on regulatory requirements, involving skin irritation, eye irritation, genotoxicity, photo-irritation, and dermal absorption, should be conducted, as the prepared formulation demonstrated relevant properties that deserve to be further explored.",
publisher = "Basel: MDPI",
journal = "Antioxidants (Basel, Switzerland)",
title = "Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development.",
number = "4",
volume = "11",
doi = "10.3390/antiox11040677",
pages = "677"
}

Besrour, N., Oludemi, T., Mandim, F., Pereira, C., Dias, M. I., Soković, M., Stojković, D., Ferreira, O., Ferreira, I. C. F. R.,& Barros, L.. (2022). Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development.. in Antioxidants (Basel, Switzerland)
Basel: MDPI., 11(4), 677.
https://doi.org/10.3390/antiox11040677

Besrour N, Oludemi T, Mandim F, Pereira C, Dias MI, Soković M, Stojković D, Ferreira O, Ferreira ICFR, Barros L. Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development.. in Antioxidants (Basel, Switzerland). 2022;11(4):677.
doi:10.3390/antiox11040677 .

Besrour, Nermine, Oludemi, Taofiq, Mandim, Filipa, Pereira, Carla, Dias, Maria Inês, Soković, Marina, Stojković, Dejan, Ferreira, Olga, Ferreira, Isabel C. F. R., Barros, Lillian, "Valorization of Juglans regia Leaves as Cosmeceutical Ingredients: Bioactivity Evaluation and Final Formulation Development." in Antioxidants (Basel, Switzerland), 11, no. 4 (2022):677,
https://doi.org/10.3390/antiox11040677 . .

TY  - JOUR
AU  - Othman, Souha
AU  - Añibarro-Ortega, Mikel
AU  - Dias, Maria Inês
AU  - Ćirić, Ana
AU  - Mandim, Filipa
AU  - Soković, Marina
AU  - Ferreira, Isabel C F R
AU  - Pinela, José
AU  - Barros, Lillian
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9587281
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5175
AB  - Quince (Cydonia oblonga Mill.) is an astringent fruit widely processed into marmalade and other sweets through processes that discard the peel as a by-product. Therefore, this study was performed to characterize the quince peel composition in nutrients and phytochemicals and evaluate its in vitro biological activity, following a "zero waste" approach. The quince peel dry powder was particularly rich in fiber (20.2 g/100 g), fructose (34 g/100 g), malic acid (7.2 g/100 g), and potassium (692 mg/100 g). Extracts prepared by dynamic hydroethanolic maceration and hot water extraction yielded 4.70 and 4.27 mg/g of phenolic compounds, respectively, with a prevalence of flavan-3-ols. The hydroethanolic extract was the most effective in inhibiting lipid peroxidation and oxidative hemolysis, and also presented better antimicrobial effects against foodborne pathogens, which agreed with the highest flavan-3-ol contents. The extracts were better than control synthetic food additives against some tested fungal and bacterial strains. On the other hand, no ability to inhibit nitric oxide production or toxicity to the tumor and non-tumor cell lines was observed. Furthermore, the solid residues remaining after extraction contained 35-37 g/100 g of fiber. Overall, quince peel can be upcycled into fiber-rich and bioactive ingredients to endow the value chain with natural food fortifiers, preservatives, and health promoters.
PB  - Elsevier Ltd.
T2  - Heliyon
T1  - Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems
IS  - 10
VL  - 8
DO  - 10.1016/j.heliyon.2022.e11042
SP  - e11042
ER  - 

@article{
author = "Othman, Souha and Añibarro-Ortega, Mikel and Dias, Maria Inês and Ćirić, Ana and Mandim, Filipa and Soković, Marina and Ferreira, Isabel C F R and Pinela, José and Barros, Lillian",
year = "2022",
abstract = "Quince (Cydonia oblonga Mill.) is an astringent fruit widely processed into marmalade and other sweets through processes that discard the peel as a by-product. Therefore, this study was performed to characterize the quince peel composition in nutrients and phytochemicals and evaluate its in vitro biological activity, following a "zero waste" approach. The quince peel dry powder was particularly rich in fiber (20.2 g/100 g), fructose (34 g/100 g), malic acid (7.2 g/100 g), and potassium (692 mg/100 g). Extracts prepared by dynamic hydroethanolic maceration and hot water extraction yielded 4.70 and 4.27 mg/g of phenolic compounds, respectively, with a prevalence of flavan-3-ols. The hydroethanolic extract was the most effective in inhibiting lipid peroxidation and oxidative hemolysis, and also presented better antimicrobial effects against foodborne pathogens, which agreed with the highest flavan-3-ol contents. The extracts were better than control synthetic food additives against some tested fungal and bacterial strains. On the other hand, no ability to inhibit nitric oxide production or toxicity to the tumor and non-tumor cell lines was observed. Furthermore, the solid residues remaining after extraction contained 35-37 g/100 g of fiber. Overall, quince peel can be upcycled into fiber-rich and bioactive ingredients to endow the value chain with natural food fortifiers, preservatives, and health promoters.",
publisher = "Elsevier Ltd.",
journal = "Heliyon",
title = "Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems",
number = "10",
volume = "8",
doi = "10.1016/j.heliyon.2022.e11042",
pages = "e11042"
}

Othman, S., Añibarro-Ortega, M., Dias, M. I., Ćirić, A., Mandim, F., Soković, M., Ferreira, I. C. F. R., Pinela, J.,& Barros, L.. (2022). Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems. in Heliyon
Elsevier Ltd.., 8(10), e11042.
https://doi.org/10.1016/j.heliyon.2022.e11042

Othman S, Añibarro-Ortega M, Dias MI, Ćirić A, Mandim F, Soković M, Ferreira ICFR, Pinela J, Barros L. Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems. in Heliyon. 2022;8(10):e11042.
doi:10.1016/j.heliyon.2022.e11042 .

Othman, Souha, Añibarro-Ortega, Mikel, Dias, Maria Inês, Ćirić, Ana, Mandim, Filipa, Soković, Marina, Ferreira, Isabel C F R, Pinela, José, Barros, Lillian, "Valorization of quince peel into functional food ingredients: A path towards "zero waste" and sustainable food systems" in Heliyon, 8, no. 10 (2022):e11042,
https://doi.org/10.1016/j.heliyon.2022.e11042 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Ferreira, Isabel C. F. R.
AU  - Santos-Buelga, Celestino
AU  - Barros, Lillian
PY  - 2022
UR  - https://www.mdpi.com/2079-7737/11/5/699
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4980
AB  - Cardoon (Cynara cardunculus var. altilis) blades were collected at sixteen sampling dates (B1-B16) to study the influence of the phenological growth stage on the phenolic composition and biological properties. Twenty phenolic compounds were identified, among which trans 3,4-O-dicaffeoylquinic acid, 5-O-caffeoylquinic acid, and luteolin-O-hexoside (39.6, 42.6, and 101.0 mg/g extract, respectively) were the main compounds. Immature blades (B3) had a higher content of phenolic compounds (178 mg/g extract) and a greater ability to inhibit the formation of thiobarbituric acid reactive substances (IC50 of 1.61 µg/mL). Samples at more advanced growth stages revealed a greater capacity to inhibit oxidative hemolysis (B8, IC50 of 25 and 47.4 µg/mL for Δt of 60 and 120 min, respectively) and higher cytotoxic (B8-B13, GI50 between 7.1 and 17 µg/mL), anti-inflammatory (B13, IC50 of 10 µg/mL), and antibacterial activities. In turn, the antifungal activity varied depending on the tested fungi. All these results suggest that maturity influences the phenolic composition and bioactive properties of cardoon blades, which reveal great potential for the development of bioactive ingredients for food and pharmaceutical applications, among others.
PB  - Basel: MDPI
T2  - Biology
T1  - Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.
IS  - 5
VL  - 11
DO  - 10.3390/biology11050699
SP  - 699
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Pinela, José and Dias, Maria Inês and Kostić, Marina and Soković, Marina and Ferreira, Isabel C. F. R. and Santos-Buelga, Celestino and Barros, Lillian",
year = "2022",
abstract = "Cardoon (Cynara cardunculus var. altilis) blades were collected at sixteen sampling dates (B1-B16) to study the influence of the phenological growth stage on the phenolic composition and biological properties. Twenty phenolic compounds were identified, among which trans 3,4-O-dicaffeoylquinic acid, 5-O-caffeoylquinic acid, and luteolin-O-hexoside (39.6, 42.6, and 101.0 mg/g extract, respectively) were the main compounds. Immature blades (B3) had a higher content of phenolic compounds (178 mg/g extract) and a greater ability to inhibit the formation of thiobarbituric acid reactive substances (IC50 of 1.61 µg/mL). Samples at more advanced growth stages revealed a greater capacity to inhibit oxidative hemolysis (B8, IC50 of 25 and 47.4 µg/mL for Δt of 60 and 120 min, respectively) and higher cytotoxic (B8-B13, GI50 between 7.1 and 17 µg/mL), anti-inflammatory (B13, IC50 of 10 µg/mL), and antibacterial activities. In turn, the antifungal activity varied depending on the tested fungi. All these results suggest that maturity influences the phenolic composition and bioactive properties of cardoon blades, which reveal great potential for the development of bioactive ingredients for food and pharmaceutical applications, among others.",
publisher = "Basel: MDPI",
journal = "Biology",
title = "Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.",
number = "5",
volume = "11",
doi = "10.3390/biology11050699",
pages = "699"
}

Mandim, F., Petropoulos, S. A., Pinela, J., Dias, M. I., Kostić, M., Soković, M., Ferreira, I. C. F. R., Santos-Buelga, C.,& Barros, L.. (2022). Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.. in Biology
Basel: MDPI., 11(5), 699.
https://doi.org/10.3390/biology11050699

Mandim F, Petropoulos SA, Pinela J, Dias MI, Kostić M, Soković M, Ferreira ICFR, Santos-Buelga C, Barros L. Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages.. in Biology. 2022;11(5):699.
doi:10.3390/biology11050699 .

Mandim, Filipa, Petropoulos, Spyridon A., Pinela, José, Dias, Maria Inês, Kostić, Marina, Soković, Marina, Ferreira, Isabel C. F. R., Santos-Buelga, Celestino, Barros, Lillian, "Phenolic Composition and Antioxidant, Anti-Inflammatory, Cytotoxic, and Antimicrobial Activities of Cardoon Blades at Different Growth Stages." in Biology, 11, no. 5 (2022):699,
https://doi.org/10.3390/biology11050699 . .

TY  - CONF
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4929
AB  - Cynara cardunculus L. (cardoon) is a typical Mediterranean species that comprises the ancestor wild
cardoon (var. sylvestris), the cultivated leafy cardoon (var. altilis) and globe artichoke (var. scolymus). It
can be found worldwide due to its high adaptability to diverse climate conditions (e.g., resistance to extreme
temperatures, water stress, and soils with variable pH) [1,2]. It is also considered a multipurpose crop due
to its nutritional, pharmacological and industrial applications [2]. Due to the increase of its commercial and
economic value, this study aimed to evaluate the polyphenolic composition and bioactive potential of
cardoon bracts in relation to plant growth cycle. Cardoon bracts were collected in central Greece at eight
different maturation stages (samples C1 – lower maturity to C8 – higher maturity). The phenolic profile was
determined by HPLC-DAD-ESI/MS. The antioxidant activity was measured through two cell-based assays:
TBARS (thiobarbituric acid reactive substances formation inhibition) and OxHLIA (oxidative hemolysis
inhibition). Cytotoxic effects were screened against four human tumor cell lines and hepatotoxicity against
a non-tumor cell line (PLP2) by the sulforhodamine B assay. The anti-inflammatory potential was tested
through the inhibition of NO production by a murine macrophage cell line (RAW 264.7). Finally, the
antibacterial and antifungal activities were evaluated by the broth microdilution method. Twelve phenolic
compounds were tentatively identified in the cardoon bract extracts and quantified in higher amounts in
immature samples. Immature bracts (C1) also revealed the highest cytotoxic (GI50 of 30 – 79 μg/mL) and
anti-inflammatory (IC50 = 72 μg/mL) activities, while they presented the highest capacity to efficiently
inhibit the formation of TBARS (IC50 = 26.8 μg/mL). In contrast, the extract with the higher maturity grade
(C7) revealed superior efficacy against oxidative hemolysis (IC50 of 38 and 75 μg/mL at Δt=60 min and 120
min, respectively). The highest antibacterial and antifungal activities were attributed to samples C1 and C6
and samples C2 and C4, respectively. The results obtained in this work could be helpful to choose the best
harvesting time of cardoon bracts, allowing obtaining a greater variety of phenolic compounds, and
consequently, a higher bioactive potential, and ultimately the most appropriate use of their constituents.
Nevertheless, further studies are needed to better understand the compounds responsible for the observed
activities, as well as to reveal the mechanisms involved in these activities.
PB  - Bragança: Instituto Politécnico de Bragança
C3  - 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online
T1  - Phenolic composition and bioactive properties of cardoon bracts: influence of the growth cycle
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4929
ER  - 

@conference{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Pinela, José and Dias, Maria Inês and Kostić, Marina and Soković, Marina and Santos-Buelga, Celestino and Ferreira, Isabel C.F.R. and Barros, Lillian",
year = "2021",
abstract = "Cynara cardunculus L. (cardoon) is a typical Mediterranean species that comprises the ancestor wild
cardoon (var. sylvestris), the cultivated leafy cardoon (var. altilis) and globe artichoke (var. scolymus). It
can be found worldwide due to its high adaptability to diverse climate conditions (e.g., resistance to extreme
temperatures, water stress, and soils with variable pH) [1,2]. It is also considered a multipurpose crop due
to its nutritional, pharmacological and industrial applications [2]. Due to the increase of its commercial and
economic value, this study aimed to evaluate the polyphenolic composition and bioactive potential of
cardoon bracts in relation to plant growth cycle. Cardoon bracts were collected in central Greece at eight
different maturation stages (samples C1 – lower maturity to C8 – higher maturity). The phenolic profile was
determined by HPLC-DAD-ESI/MS. The antioxidant activity was measured through two cell-based assays:
TBARS (thiobarbituric acid reactive substances formation inhibition) and OxHLIA (oxidative hemolysis
inhibition). Cytotoxic effects were screened against four human tumor cell lines and hepatotoxicity against
a non-tumor cell line (PLP2) by the sulforhodamine B assay. The anti-inflammatory potential was tested
through the inhibition of NO production by a murine macrophage cell line (RAW 264.7). Finally, the
antibacterial and antifungal activities were evaluated by the broth microdilution method. Twelve phenolic
compounds were tentatively identified in the cardoon bract extracts and quantified in higher amounts in
immature samples. Immature bracts (C1) also revealed the highest cytotoxic (GI50 of 30 – 79 μg/mL) and
anti-inflammatory (IC50 = 72 μg/mL) activities, while they presented the highest capacity to efficiently
inhibit the formation of TBARS (IC50 = 26.8 μg/mL). In contrast, the extract with the higher maturity grade
(C7) revealed superior efficacy against oxidative hemolysis (IC50 of 38 and 75 μg/mL at Δt=60 min and 120
min, respectively). The highest antibacterial and antifungal activities were attributed to samples C1 and C6
and samples C2 and C4, respectively. The results obtained in this work could be helpful to choose the best
harvesting time of cardoon bracts, allowing obtaining a greater variety of phenolic compounds, and
consequently, a higher bioactive potential, and ultimately the most appropriate use of their constituents.
Nevertheless, further studies are needed to better understand the compounds responsible for the observed
activities, as well as to reveal the mechanisms involved in these activities.",
publisher = "Bragança: Instituto Politécnico de Bragança",
journal = "1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online",
title = "Phenolic composition and bioactive properties of cardoon bracts: influence of the growth cycle",
pages = "47",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4929"
}

Mandim, F., Petropoulos, S. A., Pinela, J., Dias, M. I., Kostić, M., Soković, M., Santos-Buelga, C., Ferreira, I. C.F.R.,& Barros, L.. (2021). Phenolic composition and bioactive properties of cardoon bracts: influence of the growth cycle. in 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online
Bragança: Instituto Politécnico de Bragança., 47.
https://hdl.handle.net/21.15107/rcub_ibiss_4929

Mandim F, Petropoulos SA, Pinela J, Dias MI, Kostić M, Soković M, Santos-Buelga C, Ferreira IC, Barros L. Phenolic composition and bioactive properties of cardoon bracts: influence of the growth cycle. in 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online. 2021;:47.
https://hdl.handle.net/21.15107/rcub_ibiss_4929 .

Mandim, Filipa, Petropoulos, Spyridon A., Pinela, José, Dias, Maria Inês, Kostić, Marina, Soković, Marina, Santos-Buelga, Celestino, Ferreira, Isabel C.F.R., Barros, Lillian, "Phenolic composition and bioactive properties of cardoon bracts: influence of the growth cycle" in 1st Congress of  Natural products application: Health, Cosmetic and Food: Book of Abstracts; 2021 Feb 4-5; Online (2021):47,
https://hdl.handle.net/21.15107/rcub_ibiss_4929 .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Giannoulis, Kyriakos D.
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Queijo, Beatriz
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814621018811
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4474
AB  - Cardoon seeds collected in Greece at four different maturity stages (samples S1 to S4) were analysed in terms of chemical composition and in vitro bioactivities. The content of phenolic compounds (six compounds in total) increased with increasing maturity, and 3,5-O-dicaffeyolquinic (14.8-33.8 mg/g extract) acid was the compound detected in higher abundance. Mature seeds (sample S4) also revealed the highest content in lipids (23 g/100 g extract) and tocopherols (29.62 mg/100 g dw) and demonstrated the highest cytotoxic (GI50 of 97-216 µg/mL) and anti-inflammatory (IC50 = 148 µg/mL) activities, and capacity to inhibit the formation of thiobarbituric acid reactive substances (TBARS) (IC50 = 5 µg/mL). Cardoon seed hydroethanolic extracts also revealed high antibacterial and antifungal potential, particularly samples S3 and S1, respectively. This study proved the multifaceted potential associated with valorisation of cardoon seeds, while their biological and chemical composition can be influenced by the maturity stage.
T2  - Food Chemistry
T1  - Chemical composition and biological activity of cardoon (Cynara cardunculus L. var. altilis) seeds harvested at different maturity stages.
VL  - 369
DO  - 10.1016/j.foodchem.2021.130875
SP  - 130875
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Pinela, José and Dias, Maria Inês and Giannoulis, Kyriakos D. and Kostić, Marina and Soković, Marina and Queijo, Beatriz and Santos-Buelga, Celestino and Ferreira, Isabel C F R and Barros, Lillian",
year = "2021",
abstract = "Cardoon seeds collected in Greece at four different maturity stages (samples S1 to S4) were analysed in terms of chemical composition and in vitro bioactivities. The content of phenolic compounds (six compounds in total) increased with increasing maturity, and 3,5-O-dicaffeyolquinic (14.8-33.8 mg/g extract) acid was the compound detected in higher abundance. Mature seeds (sample S4) also revealed the highest content in lipids (23 g/100 g extract) and tocopherols (29.62 mg/100 g dw) and demonstrated the highest cytotoxic (GI50 of 97-216 µg/mL) and anti-inflammatory (IC50 = 148 µg/mL) activities, and capacity to inhibit the formation of thiobarbituric acid reactive substances (TBARS) (IC50 = 5 µg/mL). Cardoon seed hydroethanolic extracts also revealed high antibacterial and antifungal potential, particularly samples S3 and S1, respectively. This study proved the multifaceted potential associated with valorisation of cardoon seeds, while their biological and chemical composition can be influenced by the maturity stage.",
journal = "Food Chemistry",
title = "Chemical composition and biological activity of cardoon (Cynara cardunculus L. var. altilis) seeds harvested at different maturity stages.",
volume = "369",
doi = "10.1016/j.foodchem.2021.130875",
pages = "130875"
}

Mandim, F., Petropoulos, S. A., Pinela, J., Dias, M. I., Giannoulis, K. D., Kostić, M., Soković, M., Queijo, B., Santos-Buelga, C., Ferreira, I. C. F. R.,& Barros, L.. (2021). Chemical composition and biological activity of cardoon (Cynara cardunculus L. var. altilis) seeds harvested at different maturity stages.. in Food Chemistry, 369, 130875.
https://doi.org/10.1016/j.foodchem.2021.130875

Mandim F, Petropoulos SA, Pinela J, Dias MI, Giannoulis KD, Kostić M, Soković M, Queijo B, Santos-Buelga C, Ferreira ICFR, Barros L. Chemical composition and biological activity of cardoon (Cynara cardunculus L. var. altilis) seeds harvested at different maturity stages.. in Food Chemistry. 2021;369:130875.
doi:10.1016/j.foodchem.2021.130875 .

Mandim, Filipa, Petropoulos, Spyridon A., Pinela, José, Dias, Maria Inês, Giannoulis, Kyriakos D., Kostić, Marina, Soković, Marina, Queijo, Beatriz, Santos-Buelga, Celestino, Ferreira, Isabel C F R, Barros, Lillian, "Chemical composition and biological activity of cardoon (Cynara cardunculus L. var. altilis) seeds harvested at different maturity stages." in Food Chemistry, 369 (2021):130875,
https://doi.org/10.1016/j.foodchem.2021.130875 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32781352
UR  - https://radar.ibiss.bg.ac.rs/123456789/3846
AB  - Cardoon (Cynara cardunculus L.) bracts were collected at different maturation stages to investigate seasonal changes in the phenolic compounds profile and in vitro bioactivities. Among the 12 phenolic compounds tentatively identified, 3,5-O-dicaffeoylquinic acid (21.83 mg/g extract) and apigenin-7-O-glucuronide (10.6 mg/g extract) were the most abundant. Immature bracts (C1: principal growth stage (PGS) 5) had the highest phenolic compounds content, and anti-inflammatory (IC50 = 72 µg/mL) and cytotoxic (GI50 of 30-79 µg/mL) activities. Moreover, extract C1 inhibited efficiently the formation of thiobarbituric acid reactive substances (TBARS; IC50 = 26.8 µg/mL), while extract C8 (PGS 8/9) was more effective against oxidative haemolysis (IC50 38 and 75 µg/mL). The highest antibacterial and antifungal activities were attributed to samples C1 and C6 (PGS 7/8) and samples C2 (PGS 5/6) and C4 (PGS 6/7), respectively. Overall, the obtained results suggest the seasonal changes of polyphenolic composition and bioactivity of cardoon bracts of variable maturity.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts.
VL  - 336
DO  - 10.1016/j.foodchem.2020.127744
SP  - 127744
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Dias, Maria Inês and Pinela, José and Kostić, Marina and Soković, Marina and Santos-Buelga, Celestino and Ferreira, Isabel C F R and Barros, Lillian",
year = "2021",
abstract = "Cardoon (Cynara cardunculus L.) bracts were collected at different maturation stages to investigate seasonal changes in the phenolic compounds profile and in vitro bioactivities. Among the 12 phenolic compounds tentatively identified, 3,5-O-dicaffeoylquinic acid (21.83 mg/g extract) and apigenin-7-O-glucuronide (10.6 mg/g extract) were the most abundant. Immature bracts (C1: principal growth stage (PGS) 5) had the highest phenolic compounds content, and anti-inflammatory (IC50 = 72 µg/mL) and cytotoxic (GI50 of 30-79 µg/mL) activities. Moreover, extract C1 inhibited efficiently the formation of thiobarbituric acid reactive substances (TBARS; IC50 = 26.8 µg/mL), while extract C8 (PGS 8/9) was more effective against oxidative haemolysis (IC50 38 and 75 µg/mL). The highest antibacterial and antifungal activities were attributed to samples C1 and C6 (PGS 7/8) and samples C2 (PGS 5/6) and C4 (PGS 6/7), respectively. Overall, the obtained results suggest the seasonal changes of polyphenolic composition and bioactivity of cardoon bracts of variable maturity.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts.",
volume = "336",
doi = "10.1016/j.foodchem.2020.127744",
pages = "127744"
}

Mandim, F., Petropoulos, S. A., Dias, M. I., Pinela, J., Kostić, M., Soković, M., Santos-Buelga, C., Ferreira, I. C. F. R.,& Barros, L.. (2021). Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts.. in Food Chemistry
Elsevier Ltd., 336, 127744.
https://doi.org/10.1016/j.foodchem.2020.127744

Mandim F, Petropoulos SA, Dias MI, Pinela J, Kostić M, Soković M, Santos-Buelga C, Ferreira ICFR, Barros L. Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts.. in Food Chemistry. 2021;336:127744.
doi:10.1016/j.foodchem.2020.127744 .

Mandim, Filipa, Petropoulos, Spyridon A., Dias, Maria Inês, Pinela, José, Kostić, Marina, Soković, Marina, Santos-Buelga, Celestino, Ferreira, Isabel C F R, Barros, Lillian, "Seasonal variation in bioactive properties and phenolic composition of cardoon (Cynara cardunculus var. altilis) bracts." in Food Chemistry, 336 (2021):127744,
https://doi.org/10.1016/j.foodchem.2020.127744 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://www.mdpi.com/2076-3921/10/12/1907
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8750300
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4741
AB  - Hydroethanolic extracts of cardoon petioles collected at sixteen growth stages (P1-P16) were characterized in terms of their phenolic composition and bioactive potential (antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities). Fifteen phenolic compounds were tentatively identified (i.e., ten phenolic acids and five flavonoid glycosides); the main compounds were 5-O-caffeoylquinic and 1,5-di-O-caffeoylquinic acids. Samples collected at early maturity (P1-P4) presented a weak positive correlation between the higher content in polyphenols (P3: 101-mg/g extract) and better inhibition capacity against thiobarbituric acid reactive substance formation (TBARS; P3: IC50 = 5.0 µg/mL). Samples at intermediate maturation stages (P9) presented higher cytotoxic and anti-inflammatory potential. Moreover, immature petioles showed greater antihemolytic (OxHLIA; P4: IC50 = 65 and 180 µg/mL for Δt of 60 and 120 min, respectively) and antibacterial activity. The antifungal activity varied depending on the maturation stage and the fungi strain. In conclusion, the maturation stage may greatly affect the polyphenols composition and content and the bioactive potential of cardoon petioles.
PB  - Basel: MDPI
T2  - Antioxidants (Basel, Switzerland)
T1  - Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage.
IS  - 12
VL  - 10
DO  - 10.3390/antiox10121907
SP  - 1907
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Dias, Maria Inês and Pinela, José and Kostić, Marina and Soković, Marina and Santos-Buelga, Celestino and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Hydroethanolic extracts of cardoon petioles collected at sixteen growth stages (P1-P16) were characterized in terms of their phenolic composition and bioactive potential (antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities). Fifteen phenolic compounds were tentatively identified (i.e., ten phenolic acids and five flavonoid glycosides); the main compounds were 5-O-caffeoylquinic and 1,5-di-O-caffeoylquinic acids. Samples collected at early maturity (P1-P4) presented a weak positive correlation between the higher content in polyphenols (P3: 101-mg/g extract) and better inhibition capacity against thiobarbituric acid reactive substance formation (TBARS; P3: IC50 = 5.0 µg/mL). Samples at intermediate maturation stages (P9) presented higher cytotoxic and anti-inflammatory potential. Moreover, immature petioles showed greater antihemolytic (OxHLIA; P4: IC50 = 65 and 180 µg/mL for Δt of 60 and 120 min, respectively) and antibacterial activity. The antifungal activity varied depending on the maturation stage and the fungi strain. In conclusion, the maturation stage may greatly affect the polyphenols composition and content and the bioactive potential of cardoon petioles.",
publisher = "Basel: MDPI",
journal = "Antioxidants (Basel, Switzerland)",
title = "Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage.",
number = "12",
volume = "10",
doi = "10.3390/antiox10121907",
pages = "1907"
}

Mandim, F., Petropoulos, S. A., Dias, M. I., Pinela, J., Kostić, M., Soković, M., Santos-Buelga, C., Ferreira, I. C. F. R.,& Barros, L.. (2021). Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage.. in Antioxidants (Basel, Switzerland)
Basel: MDPI., 10(12), 1907.
https://doi.org/10.3390/antiox10121907

Mandim F, Petropoulos SA, Dias MI, Pinela J, Kostić M, Soković M, Santos-Buelga C, Ferreira ICFR, Barros L. Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage.. in Antioxidants (Basel, Switzerland). 2021;10(12):1907.
doi:10.3390/antiox10121907 .

Mandim, Filipa, Petropoulos, Spyridon A., Dias, Maria Inês, Pinela, José, Kostić, Marina, Soković, Marina, Santos-Buelga, Celestino, Ferreira, Isabel C. F. R., Barros, Lillian, "Phenolic Composition and Biological Properties of Cynara cardunculus L. var. altilis Petioles: Influence of the Maturity Stage." in Antioxidants (Basel, Switzerland), 10, no. 12 (2021):1907,
https://doi.org/10.3390/antiox10121907 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Petropoulos, Spyridon A.
AU  - Giannoulis, Kyriakos D.
AU  - Dias, Maria Inês
AU  - Fernandes, Ângela
AU  - Pinela, José
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Santos-Buelga, Celestino
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0963996920303069
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3674
AB  - Cynara cardunculus L. (cardoon) has several health benefits mainly attributed to its abundance in polyphenols. In this study, cardoon heads (capitula) were harvested in Greece during the flowering stage, and the hydroethanolic extracts were assessed in terms of phenolic compounds composition and antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities. The phenolic profile was evaluated by HPLC-DAD-ESI/MS to better understand the seasonal changes in the individual compound levels and how these changes correlate with bioactive properties. The main phenolic compounds identified were caffeoylquinic and dicaffeoylquinic acid derivatives. Immature heads (Car A: principal growth stage (PGS) 5) had the highest phenolic content (34.3 mg/g) and cytotoxic (GI50 of 69–268 µg/mL) and anti-inflammatory (IC50 of 183 µg/mL) activities. Sample Car D (PGS 6/7) revealed the highest antioxidant (IC50 of 23–227 µg/mL) and antifungal (MIC of 0.26–0.51 mg/mL) potential. Regarding the antibacterial activity, Car E (PGS 7) revealed the best results (MIC of 0.59–1.18 mg/mL). This study suggests that the maturity stage of the plant influences the phenolic composition and bioactivity.
PB  - Elsevier Ltd
T2  - Food Research International
T1  - Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis
VL  - 134
DO  - 10.1016/j.foodres.2020.109281
SP  - 109281
ER  - 

@article{
author = "Mandim, Filipa and Petropoulos, Spyridon A. and Giannoulis, Kyriakos D. and Dias, Maria Inês and Fernandes, Ângela and Pinela, José and Kostić, Marina and Soković, Marina and Barros, Lillian and Santos-Buelga, Celestino and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "Cynara cardunculus L. (cardoon) has several health benefits mainly attributed to its abundance in polyphenols. In this study, cardoon heads (capitula) were harvested in Greece during the flowering stage, and the hydroethanolic extracts were assessed in terms of phenolic compounds composition and antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities. The phenolic profile was evaluated by HPLC-DAD-ESI/MS to better understand the seasonal changes in the individual compound levels and how these changes correlate with bioactive properties. The main phenolic compounds identified were caffeoylquinic and dicaffeoylquinic acid derivatives. Immature heads (Car A: principal growth stage (PGS) 5) had the highest phenolic content (34.3 mg/g) and cytotoxic (GI50 of 69–268 µg/mL) and anti-inflammatory (IC50 of 183 µg/mL) activities. Sample Car D (PGS 6/7) revealed the highest antioxidant (IC50 of 23–227 µg/mL) and antifungal (MIC of 0.26–0.51 mg/mL) potential. Regarding the antibacterial activity, Car E (PGS 7) revealed the best results (MIC of 0.59–1.18 mg/mL). This study suggests that the maturity stage of the plant influences the phenolic composition and bioactivity.",
publisher = "Elsevier Ltd",
journal = "Food Research International",
title = "Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis",
volume = "134",
doi = "10.1016/j.foodres.2020.109281",
pages = "109281"
}

Mandim, F., Petropoulos, S. A., Giannoulis, K. D., Dias, M. I., Fernandes, Â., Pinela, J., Kostić, M., Soković, M., Barros, L., Santos-Buelga, C.,& Ferreira, I. C.F.R.. (2020). Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis. in Food Research International
Elsevier Ltd., 134, 109281.
https://doi.org/10.1016/j.foodres.2020.109281

Mandim F, Petropoulos SA, Giannoulis KD, Dias MI, Fernandes Â, Pinela J, Kostić M, Soković M, Barros L, Santos-Buelga C, Ferreira IC. Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis. in Food Research International. 2020;134:109281.
doi:10.1016/j.foodres.2020.109281 .

Mandim, Filipa, Petropoulos, Spyridon A., Giannoulis, Kyriakos D., Dias, Maria Inês, Fernandes, Ângela, Pinela, José, Kostić, Marina, Soković, Marina, Barros, Lillian, Santos-Buelga, Celestino, Ferreira, Isabel C.F.R., "Seasonal variation of bioactive properties and phenolic composition of Cynara cardunculus var. altilis" in Food Research International, 134 (2020):109281,
https://doi.org/10.1016/j.foodres.2020.109281 . .

TY  - JOUR
AU  - Mandim, Filipa
AU  - Dias, Maria Inês
AU  - Pinela, José
AU  - Barracosa, Paulo
AU  - Ivanov, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Santos-Buelga, Celestino
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3659
AB  - Cardoon is a multi-purpose crop for several industries. In this study, cardoon seeds were separated according to the viability and characterized for their chemical composition and bioactivities. Viable seeds contained higher levels of α-tocopherol (6.7 mg/100 g), lipids (23.11 g/100 g, manly oleic and linoleic acids), and free sugars (5.4 g/100 g) than the unviable ones. The hydroethanolic extract of viable seeds presented a higher concentration of phenolic compounds, namely 5-O-caffeoylquinic (8.0 mg/g) and 3,5-O-dicaffeoylquinic (43.9 mg/g) acids, and greater in vitro antioxidant activity. Both extracts showed antibacterial properties, but the best results were achieved with unviable seeds. The extracts had similar antifungal activity but did not reveal anti-inflammatory capacity or cytotoxicity to the tested cell lines. Therefore, while viable seeds contained antioxidant phytochemicals and an energy and carbon source for germination, unavailable seeds stood out for their potential to be used in the development of bio-based antibacterial ingredients.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Chemical composition and in vitro biological activities of cardoon (Cynara cardunculus L. var. altilis DC.) seeds as influenced by viability: Chemical prospection and bioactivity of cardoon seeds
VL  - 323
DO  - 10.1016/j.foodchem.2020.126838
SP  - 126838
ER  - 

@article{
author = "Mandim, Filipa and Dias, Maria Inês and Pinela, José and Barracosa, Paulo and Ivanov, Marija and Stojković, Dejan and Soković, Marina and Santos-Buelga, Celestino and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "Cardoon is a multi-purpose crop for several industries. In this study, cardoon seeds were separated according to the viability and characterized for their chemical composition and bioactivities. Viable seeds contained higher levels of α-tocopherol (6.7 mg/100 g), lipids (23.11 g/100 g, manly oleic and linoleic acids), and free sugars (5.4 g/100 g) than the unviable ones. The hydroethanolic extract of viable seeds presented a higher concentration of phenolic compounds, namely 5-O-caffeoylquinic (8.0 mg/g) and 3,5-O-dicaffeoylquinic (43.9 mg/g) acids, and greater in vitro antioxidant activity. Both extracts showed antibacterial properties, but the best results were achieved with unviable seeds. The extracts had similar antifungal activity but did not reveal anti-inflammatory capacity or cytotoxicity to the tested cell lines. Therefore, while viable seeds contained antioxidant phytochemicals and an energy and carbon source for germination, unavailable seeds stood out for their potential to be used in the development of bio-based antibacterial ingredients.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Chemical composition and in vitro biological activities of cardoon (Cynara cardunculus L. var. altilis DC.) seeds as influenced by viability: Chemical prospection and bioactivity of cardoon seeds",
volume = "323",
doi = "10.1016/j.foodchem.2020.126838",
pages = "126838"
}

Mandim, F., Dias, M. I., Pinela, J., Barracosa, P., Ivanov, M., Stojković, D., Soković, M., Santos-Buelga, C., Barros, L.,& Ferreira, I. C.F.R.. (2020). Chemical composition and in vitro biological activities of cardoon (Cynara cardunculus L. var. altilis DC.) seeds as influenced by viability: Chemical prospection and bioactivity of cardoon seeds. in Food Chemistry
Elsevier Ltd., 323, 126838.
https://doi.org/10.1016/j.foodchem.2020.126838

Mandim F, Dias MI, Pinela J, Barracosa P, Ivanov M, Stojković D, Soković M, Santos-Buelga C, Barros L, Ferreira IC. Chemical composition and in vitro biological activities of cardoon (Cynara cardunculus L. var. altilis DC.) seeds as influenced by viability: Chemical prospection and bioactivity of cardoon seeds. in Food Chemistry. 2020;323:126838.
doi:10.1016/j.foodchem.2020.126838 .

Mandim, Filipa, Dias, Maria Inês, Pinela, José, Barracosa, Paulo, Ivanov, Marija, Stojković, Dejan, Soković, Marina, Santos-Buelga, Celestino, Barros, Lillian, Ferreira, Isabel C.F.R., "Chemical composition and in vitro biological activities of cardoon (Cynara cardunculus L. var. altilis DC.) seeds as influenced by viability: Chemical prospection and bioactivity of cardoon seeds" in Food Chemistry, 323 (2020):126838,
https://doi.org/10.1016/j.foodchem.2020.126838 . .