TY  - JOUR
AU  - Li, Hanluo
AU  - Ziemer, Mirjana
AU  - Stojanović, Ivana D.
AU  - Saksida, Tamara
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Đmura, Goran
AU  - Mićanović, Dragica
AU  - Koprivica, Ivan
AU  - Krajnović, Tamara
AU  - Drača, Dijana
AU  - Simon, Jan-Christoph
AU  - Lethaus, Bernd
AU  - Savković, Vuk
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4775
AB  - Wound  healing  of  acute  full-thickness  injuries  and  chronic  non-healing  ulcers  leads  to  delayed  wound  closure,  prolonged recovery period and hypertrophic scarring, generating a demand for an autologous cell therapy and a relevant pre-clinical research  models for  wound healing.  In this study, an  immunocompetent model  for  wound  healing  was  employed  using a syngeneic murine cell line of mesenchymal stem cells cultured from the mouse whisker hair follicle outer root sheath (named moMSCORS). moMSCORS were isolated using an air-liquid interface method, expanded  in vitro  and characterized according to the MSC definition criteria - cell viability,  in vitro  proliferation, MSC phenotype and multi-lineage differentiations. Moreover, upon applying moMSCORS in an  in vivo  full-thickness wound model in the syngeneic C57BL/6 mice, the treated wounds displayed different morphology to that of the untreated wound beds. Quantitative evaluation of angiogenesis, granulation and wound closure involving clinical scoring and software-based quantification indicated a lower degree of inflammation in the treated wounds. Histological staining of treated wounds by the means of H&E, Alcian Blue, PicroSirius Red and αSMA immune labelling showed lower cellularity, less collagen filaments as well as thinner dermal and epidermal layers compared with the untreated wounds, indicating a general reduction of hypertrophic scars. The decreased inflammation, accelerated wound closure and non-hypertrophic scarring, which were facilitated by moMSCORS, hereby address a common problem of hypertrophic scars and non-physiological tissue properties upon wound closure, and additionally offer an  in  vivo  model  for  the  autologous  cell-based  wound  healing.
PB  - Basel: Springer Nature Switzerland AG
T2  - Stem Cell Reviews and Reports
T1  - Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine  Wound Healing Model
DO  - 10.1007/s12015-021-10288-7
ER  - 

@article{
author = "Li, Hanluo and Ziemer, Mirjana and Stojanović, Ivana D. and Saksida, Tamara and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Đmura, Goran and Mićanović, Dragica and Koprivica, Ivan and Krajnović, Tamara and Drača, Dijana and Simon, Jan-Christoph and Lethaus, Bernd and Savković, Vuk",
year = "2022",
abstract = "Wound  healing  of  acute  full-thickness  injuries  and  chronic  non-healing  ulcers  leads  to  delayed  wound  closure,  prolonged recovery period and hypertrophic scarring, generating a demand for an autologous cell therapy and a relevant pre-clinical research  models for  wound healing.  In this study, an  immunocompetent model  for  wound  healing  was  employed  using a syngeneic murine cell line of mesenchymal stem cells cultured from the mouse whisker hair follicle outer root sheath (named moMSCORS). moMSCORS were isolated using an air-liquid interface method, expanded  in vitro  and characterized according to the MSC definition criteria - cell viability,  in vitro  proliferation, MSC phenotype and multi-lineage differentiations. Moreover, upon applying moMSCORS in an  in vivo  full-thickness wound model in the syngeneic C57BL/6 mice, the treated wounds displayed different morphology to that of the untreated wound beds. Quantitative evaluation of angiogenesis, granulation and wound closure involving clinical scoring and software-based quantification indicated a lower degree of inflammation in the treated wounds. Histological staining of treated wounds by the means of H&E, Alcian Blue, PicroSirius Red and αSMA immune labelling showed lower cellularity, less collagen filaments as well as thinner dermal and epidermal layers compared with the untreated wounds, indicating a general reduction of hypertrophic scars. The decreased inflammation, accelerated wound closure and non-hypertrophic scarring, which were facilitated by moMSCORS, hereby address a common problem of hypertrophic scars and non-physiological tissue properties upon wound closure, and additionally offer an  in  vivo  model  for  the  autologous  cell-based  wound  healing.",
publisher = "Basel: Springer Nature Switzerland AG",
journal = "Stem Cell Reviews and Reports",
title = "Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine  Wound Healing Model",
doi = "10.1007/s12015-021-10288-7"
}

Li, H., Ziemer, M., Stojanović, I. D., Saksida, T., Maksimović-Ivanić, D., Mijatović, S., Đmura, G., Mićanović, D., Koprivica, I., Krajnović, T., Drača, D., Simon, J., Lethaus, B.,& Savković, V.. (2022). Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine  Wound Healing Model. in Stem Cell Reviews and Reports
Basel: Springer Nature Switzerland AG..
https://doi.org/10.1007/s12015-021-10288-7

Li H, Ziemer M, Stojanović ID, Saksida T, Maksimović-Ivanić D, Mijatović S, Đmura G, Mićanović D, Koprivica I, Krajnović T, Drača D, Simon J, Lethaus B, Savković V. Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine  Wound Healing Model. in Stem Cell Reviews and Reports. 2022;.
doi:10.1007/s12015-021-10288-7 .

Li, Hanluo, Ziemer, Mirjana, Stojanović, Ivana D., Saksida, Tamara, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Đmura, Goran, Mićanović, Dragica, Koprivica, Ivan, Krajnović, Tamara, Drača, Dijana, Simon, Jan-Christoph, Lethaus, Bernd, Savković, Vuk, "Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine  Wound Healing Model" in Stem Cell Reviews and Reports (2022),
https://doi.org/10.1007/s12015-021-10288-7 . .

TY  - JOUR
AU  - Li, Hanluo
AU  - Masieri, Federica Francesca
AU  - Schneider, Marie
AU  - Bartella, Alexander
AU  - Gaus, Sebastian
AU  - Hahnel, Sebastian
AU  - Zimmerer, Rüdiger
AU  - Sack, Ulrich
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Simon, Jan-Christoph
AU  - Lethaus, Bernd
AU  - Savković, Vuk
PY  - 2021
UR  - internal-pdf://Li et al. - 2021 - The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem.pdf
UR  - https://www.mdpi.com/2218-273X/11/2/154
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4149
AB  - Hair follicle outer root sheath (ORS) is a putative source of stem cells with therapeutic capacity. ORS contains several multipotent stem cell populations, primarily in the distal compartment of the bulge region. However, the bulge is routinely obtained using invasive isolation methods, which require human scalp tissue ex vivo. Non-invasive sampling has been standardized by means of the plucking procedure, enabling to reproducibly obtain the mid-ORS part. The mid-ORS shows potential for giving rise to multiple stem cell populations in vitro. To demonstrate the phenotypic features of distal, middle, and proximal ORS parts, gene and protein expression profiles were studied in physically separated portions. The mid-part of the ORS showed a comparable or higher NGFR, nestin/NES, CD34, CD73, CD44, CD133, CK5, PAX3, MITF, and PMEL expression on both protein and gene levels, when compared to the distal ORS part. Distinct subpopulations of cells exhibiting small and round morphology were characterized with flow cytometry as simultaneously expressing CD73/CD271, CD49f/CD105, nestin, and not CK10. Potentially, these distinct subpopulations can give rise to cultured neuroectodermal and mesenchymal stem cell populations in vitro. In conclusion, the mid part of the ORS holds the potential for yielding multiple stem cells, in particular mesenchymal stem cells.
PB  - MDPI AG
T2  - Biomolecules
T1  - The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers
IS  - 2
VL  - 11
DO  - 10.3390/biom11020154
SP  - 154
ER  - 

@article{
author = "Li, Hanluo and Masieri, Federica Francesca and Schneider, Marie and Bartella, Alexander and Gaus, Sebastian and Hahnel, Sebastian and Zimmerer, Rüdiger and Sack, Ulrich and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Simon, Jan-Christoph and Lethaus, Bernd and Savković, Vuk",
year = "2021",
abstract = "Hair follicle outer root sheath (ORS) is a putative source of stem cells with therapeutic capacity. ORS contains several multipotent stem cell populations, primarily in the distal compartment of the bulge region. However, the bulge is routinely obtained using invasive isolation methods, which require human scalp tissue ex vivo. Non-invasive sampling has been standardized by means of the plucking procedure, enabling to reproducibly obtain the mid-ORS part. The mid-ORS shows potential for giving rise to multiple stem cell populations in vitro. To demonstrate the phenotypic features of distal, middle, and proximal ORS parts, gene and protein expression profiles were studied in physically separated portions. The mid-part of the ORS showed a comparable or higher NGFR, nestin/NES, CD34, CD73, CD44, CD133, CK5, PAX3, MITF, and PMEL expression on both protein and gene levels, when compared to the distal ORS part. Distinct subpopulations of cells exhibiting small and round morphology were characterized with flow cytometry as simultaneously expressing CD73/CD271, CD49f/CD105, nestin, and not CK10. Potentially, these distinct subpopulations can give rise to cultured neuroectodermal and mesenchymal stem cell populations in vitro. In conclusion, the mid part of the ORS holds the potential for yielding multiple stem cells, in particular mesenchymal stem cells.",
publisher = "MDPI AG",
journal = "Biomolecules",
title = "The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers",
number = "2",
volume = "11",
doi = "10.3390/biom11020154",
pages = "154"
}

Li, H., Masieri, F. F., Schneider, M., Bartella, A., Gaus, S., Hahnel, S., Zimmerer, R., Sack, U., Maksimović-Ivanić, D., Mijatović, S., Simon, J., Lethaus, B.,& Savković, V.. (2021). The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers. in Biomolecules
MDPI AG., 11(2), 154.
https://doi.org/10.3390/biom11020154

Li H, Masieri FF, Schneider M, Bartella A, Gaus S, Hahnel S, Zimmerer R, Sack U, Maksimović-Ivanić D, Mijatović S, Simon J, Lethaus B, Savković V. The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers. in Biomolecules. 2021;11(2):154.
doi:10.3390/biom11020154 .

Li, Hanluo, Masieri, Federica Francesca, Schneider, Marie, Bartella, Alexander, Gaus, Sebastian, Hahnel, Sebastian, Zimmerer, Rüdiger, Sack, Ulrich, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Simon, Jan-Christoph, Lethaus, Bernd, Savković, Vuk, "The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers" in Biomolecules, 11, no. 2 (2021):154,
https://doi.org/10.3390/biom11020154 . .