TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Jarić, Ivana
AU  - Živanović, Jasmina
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Ristić, Nataša
AU  - Stanković, Sanja D.
AU  - Milošević, Verica
PY  - 2016
UR  - http://www.ncbi.nlm.nih.gov/pubmed/26951517
UR  - http://www.hh.um.es/Abstracts/Vol_31/31_11/31_11_1209.htm
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2469
AB  - Histological analysis of the adrenal cortex, after testosterone application in a rat model of the andropause, was the main subject of the present study. Middle-aged Wistar rats were divided into sham-operated (SO; n=8), orchidectomized (Orx; n=8) and testosterone treated orchidectomized (Orx+T; n=8) groups. Testosterone propionate (5 mg/kg b.w. /day) was administered for three weeks, while SO and Orx groups received the vehicle alone. Histological objectives were achieved using stereology, histochemistry and steroid receptor immunostaining. The concentrations of testosterone, aldosterone, corticosterone and DHEA were determined by immunoassays. Expectedly, increased (p<0.05) serum concentration of testosterone was observed in Orx+T group. The volume of ZG cells and nuclei increased in Orx+T animals by 50% and 25% (p<0.05) respectively, but the serum concentrations of aldosterone decreased (p<0.05) by 60%, all compared to the same parameters in Orx group. The immunostaining for androgen receptors (ARs) suggested their cytoplasmic localization in ZG cells of Orx+T rats. Volume of the ZF cell nuclei in Orx+T group decreased (p<0.05) by 17%, which was followed by the significant (p<0.05) fall in corticosterone production and secretion, all in comparison with Orx animals. Also, nuclear immunolocalization of ARs of high optical density was observed through the ZF of Orx+T group. In Orx+T rats volume of ZR cells and nuclei, and circulating DHEA concentration increased (p<0.05) by 68%, 22% and about 6.6 times respectively, compared to Orx animals. Besides the extra-receptor actions in adrenal cortex, testosterone supposedly affects some steroidogenesis-related gene expression, as indicated by centripetal rise in the number of nuclear ARs.
PB  - Sociedad Española de Histología e Ingeniería Tisular, University of Murcia
T2  - Histology and Histopathology
T1  - Histological parameters of the adrenal cortex after testosterone application in a rat model of the andropause
IS  - 11
VL  - 31
DO  - 10.14670/HH-11-749
SP  - 1209
EP  - 20
ER  - 

@article{
author = "Ajdžanović, Vladimir and Jarić, Ivana and Živanović, Jasmina and Filipović, Branko and Šošić-Jurjević, Branka  and Ristić, Nataša and Stanković, Sanja D. and Milošević, Verica",
year = "2016",
abstract = "Histological analysis of the adrenal cortex, after testosterone application in a rat model of the andropause, was the main subject of the present study. Middle-aged Wistar rats were divided into sham-operated (SO; n=8), orchidectomized (Orx; n=8) and testosterone treated orchidectomized (Orx+T; n=8) groups. Testosterone propionate (5 mg/kg b.w. /day) was administered for three weeks, while SO and Orx groups received the vehicle alone. Histological objectives were achieved using stereology, histochemistry and steroid receptor immunostaining. The concentrations of testosterone, aldosterone, corticosterone and DHEA were determined by immunoassays. Expectedly, increased (p<0.05) serum concentration of testosterone was observed in Orx+T group. The volume of ZG cells and nuclei increased in Orx+T animals by 50% and 25% (p<0.05) respectively, but the serum concentrations of aldosterone decreased (p<0.05) by 60%, all compared to the same parameters in Orx group. The immunostaining for androgen receptors (ARs) suggested their cytoplasmic localization in ZG cells of Orx+T rats. Volume of the ZF cell nuclei in Orx+T group decreased (p<0.05) by 17%, which was followed by the significant (p<0.05) fall in corticosterone production and secretion, all in comparison with Orx animals. Also, nuclear immunolocalization of ARs of high optical density was observed through the ZF of Orx+T group. In Orx+T rats volume of ZR cells and nuclei, and circulating DHEA concentration increased (p<0.05) by 68%, 22% and about 6.6 times respectively, compared to Orx animals. Besides the extra-receptor actions in adrenal cortex, testosterone supposedly affects some steroidogenesis-related gene expression, as indicated by centripetal rise in the number of nuclear ARs.",
publisher = "Sociedad Española de Histología e Ingeniería Tisular, University of Murcia",
journal = "Histology and Histopathology",
title = "Histological parameters of the adrenal cortex after testosterone application in a rat model of the andropause",
number = "11",
volume = "31",
doi = "10.14670/HH-11-749",
pages = "1209-20"
}

Ajdžanović, V., Jarić, I., Živanović, J., Filipović, B., Šošić-Jurjević, B., Ristić, N., Stanković, S. D.,& Milošević, V.. (2016). Histological parameters of the adrenal cortex after testosterone application in a rat model of the andropause. in Histology and Histopathology
Sociedad Española de Histología e Ingeniería Tisular, University of Murcia., 31(11), 1209-20.
https://doi.org/10.14670/HH-11-749

Ajdžanović V, Jarić I, Živanović J, Filipović B, Šošić-Jurjević B, Ristić N, Stanković SD, Milošević V. Histological parameters of the adrenal cortex after testosterone application in a rat model of the andropause. in Histology and Histopathology. 2016;31(11):1209-20.
doi:10.14670/HH-11-749 .

Ajdžanović, Vladimir, Jarić, Ivana, Živanović, Jasmina, Filipović, Branko, Šošić-Jurjević, Branka , Ristić, Nataša, Stanković, Sanja D., Milošević, Verica, "Histological parameters of the adrenal cortex after testosterone application in a rat model of the andropause" in Histology and Histopathology, 31, no. 11 (2016):1209-20,
https://doi.org/10.14670/HH-11-749 . .