TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana
AU  - Milinčić, Danijel
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6687
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
VL  - 17
DO  - 10.3390/ph17040518
SP  - 518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana and Milinčić, Danijel and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
volume = "17",
doi = "10.3390/ph17040518",
pages = "518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M., Milinčić, D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
Basel: MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić M, Milinčić D, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana, Milinčić, Danijel, Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - JOUR
AU  - Čutović, Natalija
AU  - Marković, Tatjana
AU  - Kostić, Marina
AU  - Gašić, Uroš
AU  - Prijić, Željana
AU  - Ren, Xiuxia
AU  - Lukić, Milan
AU  - Bugarski, Branko
PY  - 2022
UR  - https://www.mdpi.com/1424-8247/15/12/1537
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5358
AB  - Without being aware of its chemical makeup, many ancient societies have used Steppe peony in their traditional medicine. Given that modern phytopreparation intended for use on human skin requires, above all, knowledge of its chemical composition, the goal of this study was to make a screening of the composition of aqueous and methanolic extracts of the petals of P. tenuifolia L. and to examine them for various skin-beneficial properties. The extracts were prepared by maceration, ultrasound-assisted, and microwave-assisted extraction procedures. The chemical profiling was conducted by the use of UHPLC-LTQ-OrbiTrap MS and UHPLC/MS, and spectrophotometric methods for the determination of total polyphenol and total flavonoid contents. The biological activities entailed antioxidant ABTS, DPPH, CUPRAC (Cupric Ion Reducing Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power) assays, antimicrobial (antibacterial and antifungal) and antibiofilm activities, cytotoxicity, wound healing potential, as well as the adhesion and invasion of Staphylococcus lugdunensis. The results showed that the petals are rich in phenolic acids and flavonoids, which are commonly associated with numerous biological activities. The aqueous extracts were more efficient in the majority of the bioactivity assays then the methanolic ones, whereas the optimal extraction method varied between the assays. This study is the first step towards the safe use of the aqueous extracts of P. tenuifolia petals for therapeutic skin treatments.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia
IS  - 12
VL  - 15
DO  - 10.3390/ph15121537
SP  - 1537
ER  - 

@article{
author = "Čutović, Natalija and Marković, Tatjana and Kostić, Marina and Gašić, Uroš and Prijić, Željana and Ren, Xiuxia and Lukić, Milan and Bugarski, Branko",
year = "2022",
abstract = "Without being aware of its chemical makeup, many ancient societies have used Steppe peony in their traditional medicine. Given that modern phytopreparation intended for use on human skin requires, above all, knowledge of its chemical composition, the goal of this study was to make a screening of the composition of aqueous and methanolic extracts of the petals of P. tenuifolia L. and to examine them for various skin-beneficial properties. The extracts were prepared by maceration, ultrasound-assisted, and microwave-assisted extraction procedures. The chemical profiling was conducted by the use of UHPLC-LTQ-OrbiTrap MS and UHPLC/MS, and spectrophotometric methods for the determination of total polyphenol and total flavonoid contents. The biological activities entailed antioxidant ABTS, DPPH, CUPRAC (Cupric Ion Reducing Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power) assays, antimicrobial (antibacterial and antifungal) and antibiofilm activities, cytotoxicity, wound healing potential, as well as the adhesion and invasion of Staphylococcus lugdunensis. The results showed that the petals are rich in phenolic acids and flavonoids, which are commonly associated with numerous biological activities. The aqueous extracts were more efficient in the majority of the bioactivity assays then the methanolic ones, whereas the optimal extraction method varied between the assays. This study is the first step towards the safe use of the aqueous extracts of P. tenuifolia petals for therapeutic skin treatments.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia",
number = "12",
volume = "15",
doi = "10.3390/ph15121537",
pages = "1537"
}

Čutović, N., Marković, T., Kostić, M., Gašić, U., Prijić, Ž., Ren, X., Lukić, M.,& Bugarski, B.. (2022). Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia. in Pharmaceuticals
Basel: MDPI., 15(12), 1537.
https://doi.org/10.3390/ph15121537

Čutović N, Marković T, Kostić M, Gašić U, Prijić Ž, Ren X, Lukić M, Bugarski B. Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia. in Pharmaceuticals. 2022;15(12):1537.
doi:10.3390/ph15121537 .

Čutović, Natalija, Marković, Tatjana, Kostić, Marina, Gašić, Uroš, Prijić, Željana, Ren, Xiuxia, Lukić, Milan, Bugarski, Branko, "Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia" in Pharmaceuticals, 15, no. 12 (2022):1537,
https://doi.org/10.3390/ph15121537 . .

TY  - CONF
AU  - Jovanović, Aleksandra
AU  - Ćujić, Danica
AU  - Gnjatović, Marija
AU  - Stepanović, Sandra
AU  - Dinić, Svetlana
AU  - Mihailović, Mirjana
AU  - Bugarski, Branko
PY  - 2022
UR  - https://icatsconf.org/ICAT22/van
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5256
AB  - In the present research, the storage and UV stability of small unilamellar liposomes (SUVs) with encapsulated
silymarin and silibinin were investigated in terms of changes in vesicle size, polydispersity index (PDI), zeta
potential, mobility, and conductivity. Silymarin and silibinin were encapsulated within phospholipid liposomes
produced using the proliposome method and subsequently sonicated. On the 1st day, vesicle size was 1681.0±55.5
nm for silymarin liposomes and 1884.7±2.5 nm for silibinin liposomes, whereas PDI was 0.321±0.012 and
0.319±0.011. Additionally, the zeta potential of SUVs with silymarin and silibinin was -38.6±0.8 and -37.6±1.2
mV, respectively, whereas mobility and conductivity were -3.03±0.06 μmcm/Vs and 0.149±0.002 mS/cm (for
silymarin sample) and -2.94±0.09 μmcm/Vs and 0.154±0.002 mS/cm (for silibinin sample). After 28 days of storage
at 4°C, there was a statistically significant increase in the vesicle size of SUVs with silymarin and silibinin
(2703.5±44.5 and 2172.5±26.2 nm) and a decrease in zeta potential (-16.7±0.5 and -9.8±0.1 mV), mobility (-
1.31±0.04 and -0.76±0.01 μmcm/Vs), and conductivity (0.027±0.001 and 0.023±0.001 mS/cm). On the other hand,
UV irradiation did not affect changes in vesicle size and PDI of all liposomes, but it caused a decrease in zeta
potential, -32.9±1.0 mV for silymarin and -33.9±0.3 mV for silibinin, mobility, -2.64±0.1 and -2.47±0.2 μmcm/Vs,
and conductivity, 0.131±0.005 and 0.075±0.006 mS/cm. Also, after 28 days of storage, UV-irradiated SUVs with
silymarin and silibinin have significantly higher vesicle size and lower zeta potential, mobility, and conductivity in
comparison to the measurements immediately after UV irradiation. In conclusion, both non-treated and UVirradiated
silymarin- and silibinin-loaded SUVs were unstable during storage at 4°C, resulting in changes in vesicle
size, zeta potential, mobility, and conductivity, thus additional experiments for improving liposomal stability should
be performed.
PB  - Çumra, Turkey: Plusbase Akademi Publishing
C3  - Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey
T1  - Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin
SP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5256
ER  - 

@conference{
author = "Jovanović, Aleksandra and Ćujić, Danica and Gnjatović, Marija and Stepanović, Sandra and Dinić, Svetlana and Mihailović, Mirjana and Bugarski, Branko",
year = "2022",
abstract = "In the present research, the storage and UV stability of small unilamellar liposomes (SUVs) with encapsulated
silymarin and silibinin were investigated in terms of changes in vesicle size, polydispersity index (PDI), zeta
potential, mobility, and conductivity. Silymarin and silibinin were encapsulated within phospholipid liposomes
produced using the proliposome method and subsequently sonicated. On the 1st day, vesicle size was 1681.0±55.5
nm for silymarin liposomes and 1884.7±2.5 nm for silibinin liposomes, whereas PDI was 0.321±0.012 and
0.319±0.011. Additionally, the zeta potential of SUVs with silymarin and silibinin was -38.6±0.8 and -37.6±1.2
mV, respectively, whereas mobility and conductivity were -3.03±0.06 μmcm/Vs and 0.149±0.002 mS/cm (for
silymarin sample) and -2.94±0.09 μmcm/Vs and 0.154±0.002 mS/cm (for silibinin sample). After 28 days of storage
at 4°C, there was a statistically significant increase in the vesicle size of SUVs with silymarin and silibinin
(2703.5±44.5 and 2172.5±26.2 nm) and a decrease in zeta potential (-16.7±0.5 and -9.8±0.1 mV), mobility (-
1.31±0.04 and -0.76±0.01 μmcm/Vs), and conductivity (0.027±0.001 and 0.023±0.001 mS/cm). On the other hand,
UV irradiation did not affect changes in vesicle size and PDI of all liposomes, but it caused a decrease in zeta
potential, -32.9±1.0 mV for silymarin and -33.9±0.3 mV for silibinin, mobility, -2.64±0.1 and -2.47±0.2 μmcm/Vs,
and conductivity, 0.131±0.005 and 0.075±0.006 mS/cm. Also, after 28 days of storage, UV-irradiated SUVs with
silymarin and silibinin have significantly higher vesicle size and lower zeta potential, mobility, and conductivity in
comparison to the measurements immediately after UV irradiation. In conclusion, both non-treated and UVirradiated
silymarin- and silibinin-loaded SUVs were unstable during storage at 4°C, resulting in changes in vesicle
size, zeta potential, mobility, and conductivity, thus additional experiments for improving liposomal stability should
be performed.",
publisher = "Çumra, Turkey: Plusbase Akademi Publishing",
journal = "Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey",
title = "Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5256"
}

Jovanović, A., Ćujić, D., Gnjatović, M., Stepanović, S., Dinić, S., Mihailović, M.,& Bugarski, B.. (2022). Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin. in Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey
Çumra, Turkey: Plusbase Akademi Publishing., 158.
https://hdl.handle.net/21.15107/rcub_ibiss_5256

Jovanović A, Ćujić D, Gnjatović M, Stepanović S, Dinić S, Mihailović M, Bugarski B. Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin. in Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey. 2022;:158.
https://hdl.handle.net/21.15107/rcub_ibiss_5256 .

Jovanović, Aleksandra, Ćujić, Danica, Gnjatović, Marija, Stepanović, Sandra, Dinić, Svetlana, Mihailović, Mirjana, Bugarski, Branko, "Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin" in Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey (2022):158,
https://hdl.handle.net/21.15107/rcub_ibiss_5256 .

TY  - JOUR
AU  - Gomes, Clarissa Pedrosa da C
AU  - Ágg, Bence
AU  - Andova, Andrejaana
AU  - Arslan, Serdal
AU  - Baker, Andrew
AU  - Barteková, Monika
AU  - Beis, Dimitris
AU  - Betsou, Fay
AU  - Wettinger, Stephanie Bezzina
AU  - Bugarski, Branko
AU  - Condorelli, Gianluigi
AU  - Silva, Gustavo José Justo da
AU  - Danilin, Sabrina
AU  - de Gonzalo-Calvo, David
AU  - Buil, Alfonso
AU  - Carmo-Fonseca, Maria
AU  - Enguita, Francisco J
AU  - Felekkis, Kyriacos
AU  - Ferdinandy, Peter
AU  - Gyöngyösi, Mariann
AU  - Hackl, Matthias
AU  - Karaduzovic-Hadziabdic, Kanita
AU  - Hellemans, Jan
AU  - Heymans, Stephane
AU  - Hlavackova, Markéta
AU  - Hoydal, Morten Andre
AU  - Janković, Aleksandra
AU  - Jusic, Amela
AU  - Kardassis, Dimitris
AU  - Kerkelä, Risto
AU  - Kuster, Gabriela M
AU  - Lakkisto, Päivi
AU  - Leszek, Przemyslaw
AU  - Lustrek, Mitja
AU  - Maegdefessel, Lars
AU  - Martelli, Fabio
AU  - Novella, Susana
AU  - O'Brien, Timothy
AU  - Papaneophytou, Christos
AU  - Pedrazzini, Thierry
AU  - Pinet, Florence
AU  - Popescu, Octavian
AU  - Potočnjak, Ines
AU  - Robinson, Emma
AU  - Sasson, Shlomo
AU  - Scholz, Markus
AU  - Simionescu, Maya
AU  - Stoll, Monika
AU  - Varga, Zoltan V
AU  - Vinciguerra, Manlio
AU  - Xuereb, Angela
AU  - Yilmaz, Mehmet Birhan
AU  - Emanueli, Costanza
AU  - Devaux, Yvan
AU  - on behalf of the EU-CardioRNA COST Action (CA17129)
PY  - 2019
UR  - https://www.mdpi.com/2311-553X/5/2/31
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6630366
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3443
AB  - Cardiovascular disease (CVD) remains the leading cause of death worldwide and, despite continuous advances, better diagnostic and prognostic tools, as well as therapy, are needed. The human transcriptome, which is the set of all RNA produced in a cell, is much more complex than previously thought and the lack of dialogue between researchers and industrials and consensus on guidelines to generate data make it harder to compare and reproduce results. This European Cooperation in Science and Technology (COST) Action aims to accelerate the understanding of transcriptomics in CVD and further the translation of experimental data into usable applications to improve personalized medicine in this field by creating an interdisciplinary network. It aims to provide opportunities for collaboration between stakeholders from complementary backgrounds, allowing the functions of different RNAs and their interactions to be more rapidly deciphered in the cardiovascular context for translation into the clinic, thus fostering personalized medicine and meeting a current public health challenge. Thus, this Action will advance studies on cardiovascular transcriptomics, generate innovative projects, and consolidate the leadership of European research groups in the field.COST (European Cooperation in Science and Technology) is a funding organization for research and innovation networks (www.cost.eu).
T2  - Non-Coding RNA
T1  - Catalyzing Transcriptomics Research in Cardiovascular Disease: The CardioRNA COST Action CA17129.
IS  - 2
VL  - 5
DO  - 10.3390/ncrna5020031
SP  - 31
ER  - 

@article{
author = "Gomes, Clarissa Pedrosa da C and Ágg, Bence and Andova, Andrejaana and Arslan, Serdal and Baker, Andrew and Barteková, Monika and Beis, Dimitris and Betsou, Fay and Wettinger, Stephanie Bezzina and Bugarski, Branko and Condorelli, Gianluigi and Silva, Gustavo José Justo da and Danilin, Sabrina and de Gonzalo-Calvo, David and Buil, Alfonso and Carmo-Fonseca, Maria and Enguita, Francisco J and Felekkis, Kyriacos and Ferdinandy, Peter and Gyöngyösi, Mariann and Hackl, Matthias and Karaduzovic-Hadziabdic, Kanita and Hellemans, Jan and Heymans, Stephane and Hlavackova, Markéta and Hoydal, Morten Andre and Janković, Aleksandra and Jusic, Amela and Kardassis, Dimitris and Kerkelä, Risto and Kuster, Gabriela M and Lakkisto, Päivi and Leszek, Przemyslaw and Lustrek, Mitja and Maegdefessel, Lars and Martelli, Fabio and Novella, Susana and O'Brien, Timothy and Papaneophytou, Christos and Pedrazzini, Thierry and Pinet, Florence and Popescu, Octavian and Potočnjak, Ines and Robinson, Emma and Sasson, Shlomo and Scholz, Markus and Simionescu, Maya and Stoll, Monika and Varga, Zoltan V and Vinciguerra, Manlio and Xuereb, Angela and Yilmaz, Mehmet Birhan and Emanueli, Costanza and Devaux, Yvan and on behalf of the EU-CardioRNA COST Action (CA17129)",
year = "2019",
abstract = "Cardiovascular disease (CVD) remains the leading cause of death worldwide and, despite continuous advances, better diagnostic and prognostic tools, as well as therapy, are needed. The human transcriptome, which is the set of all RNA produced in a cell, is much more complex than previously thought and the lack of dialogue between researchers and industrials and consensus on guidelines to generate data make it harder to compare and reproduce results. This European Cooperation in Science and Technology (COST) Action aims to accelerate the understanding of transcriptomics in CVD and further the translation of experimental data into usable applications to improve personalized medicine in this field by creating an interdisciplinary network. It aims to provide opportunities for collaboration between stakeholders from complementary backgrounds, allowing the functions of different RNAs and their interactions to be more rapidly deciphered in the cardiovascular context for translation into the clinic, thus fostering personalized medicine and meeting a current public health challenge. Thus, this Action will advance studies on cardiovascular transcriptomics, generate innovative projects, and consolidate the leadership of European research groups in the field.COST (European Cooperation in Science and Technology) is a funding organization for research and innovation networks (www.cost.eu).",
journal = "Non-Coding RNA",
title = "Catalyzing Transcriptomics Research in Cardiovascular Disease: The CardioRNA COST Action CA17129.",
number = "2",
volume = "5",
doi = "10.3390/ncrna5020031",
pages = "31"
}

Gomes, C. P. d. C., Ágg, B., Andova, A., Arslan, S., Baker, A., Barteková, M., Beis, D., Betsou, F., Wettinger, S. B., Bugarski, B., Condorelli, G., Silva, G. J. J. d., Danilin, S., de Gonzalo-Calvo, D., Buil, A., Carmo-Fonseca, M., Enguita, F. J., Felekkis, K., Ferdinandy, P., Gyöngyösi, M., Hackl, M., Karaduzovic-Hadziabdic, K., Hellemans, J., Heymans, S., Hlavackova, M., Hoydal, M. A., Janković, A., Jusic, A., Kardassis, D., Kerkelä, R., Kuster, G. M., Lakkisto, P., Leszek, P., Lustrek, M., Maegdefessel, L., Martelli, F., Novella, S., O'Brien, T., Papaneophytou, C., Pedrazzini, T., Pinet, F., Popescu, O., Potočnjak, I., Robinson, E., Sasson, S., Scholz, M., Simionescu, M., Stoll, M., Varga, Z. V., Vinciguerra, M., Xuereb, A., Yilmaz, M. B., Emanueli, C., Devaux, Y.,& on behalf of the EU-CardioRNA COST Action (CA17129). (2019). Catalyzing Transcriptomics Research in Cardiovascular Disease: The CardioRNA COST Action CA17129.. in Non-Coding RNA, 5(2), 31.
https://doi.org/10.3390/ncrna5020031

Gomes CPDC, Ágg B, Andova A, Arslan S, Baker A, Barteková M, Beis D, Betsou F, Wettinger SB, Bugarski B, Condorelli G, Silva GJJD, Danilin S, de Gonzalo-Calvo D, Buil A, Carmo-Fonseca M, Enguita FJ, Felekkis K, Ferdinandy P, Gyöngyösi M, Hackl M, Karaduzovic-Hadziabdic K, Hellemans J, Heymans S, Hlavackova M, Hoydal MA, Janković A, Jusic A, Kardassis D, Kerkelä R, Kuster GM, Lakkisto P, Leszek P, Lustrek M, Maegdefessel L, Martelli F, Novella S, O'Brien T, Papaneophytou C, Pedrazzini T, Pinet F, Popescu O, Potočnjak I, Robinson E, Sasson S, Scholz M, Simionescu M, Stoll M, Varga ZV, Vinciguerra M, Xuereb A, Yilmaz MB, Emanueli C, Devaux Y, on behalf of the EU-CardioRNA COST Action (CA17129). Catalyzing Transcriptomics Research in Cardiovascular Disease: The CardioRNA COST Action CA17129.. in Non-Coding RNA. 2019;5(2):31.
doi:10.3390/ncrna5020031 .

Gomes, Clarissa Pedrosa da C, Ágg, Bence, Andova, Andrejaana, Arslan, Serdal, Baker, Andrew, Barteková, Monika, Beis, Dimitris, Betsou, Fay, Wettinger, Stephanie Bezzina, Bugarski, Branko, Condorelli, Gianluigi, Silva, Gustavo José Justo da, Danilin, Sabrina, de Gonzalo-Calvo, David, Buil, Alfonso, Carmo-Fonseca, Maria, Enguita, Francisco J, Felekkis, Kyriacos, Ferdinandy, Peter, Gyöngyösi, Mariann, Hackl, Matthias, Karaduzovic-Hadziabdic, Kanita, Hellemans, Jan, Heymans, Stephane, Hlavackova, Markéta, Hoydal, Morten Andre, Janković, Aleksandra, Jusic, Amela, Kardassis, Dimitris, Kerkelä, Risto, Kuster, Gabriela M, Lakkisto, Päivi, Leszek, Przemyslaw, Lustrek, Mitja, Maegdefessel, Lars, Martelli, Fabio, Novella, Susana, O'Brien, Timothy, Papaneophytou, Christos, Pedrazzini, Thierry, Pinet, Florence, Popescu, Octavian, Potočnjak, Ines, Robinson, Emma, Sasson, Shlomo, Scholz, Markus, Simionescu, Maya, Stoll, Monika, Varga, Zoltan V, Vinciguerra, Manlio, Xuereb, Angela, Yilmaz, Mehmet Birhan, Emanueli, Costanza, Devaux, Yvan, on behalf of the EU-CardioRNA COST Action (CA17129), "Catalyzing Transcriptomics Research in Cardiovascular Disease: The CardioRNA COST Action CA17129." in Non-Coding RNA, 5, no. 2 (2019):31,
https://doi.org/10.3390/ncrna5020031 . .

TY  - JOUR
AU  - Bukara, Katarina
AU  - Drvenica, Ivana
AU  - Ilić, Vesna
AU  - Stančić, Ana
AU  - Mišić, Danijela
AU  - Vasić, Borislav
AU  - Gajić, Radoš
AU  - Vučetić, Dušan
AU  - Kiekens, Filip
AU  - Bugarski, Branko
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0168165616315735
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-84992192143&origin=SingleRecordEmailAlert&txGid=694651161B2E63C5600F6DE538B0ACEC.wsnAw8kcdt7IPYLO0V48gA:3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2525
AB  - The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials – porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process – gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.
T2  - Journal of Biotechnology
T1  - Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts
VL  - 240
DO  - 10.1016/j.jbiotec.2016.10.017
SP  - 14
EP  - 22
ER  - 

@article{
author = "Bukara, Katarina and Drvenica, Ivana and Ilić, Vesna and Stančić, Ana and Mišić, Danijela and Vasić, Borislav and Gajić, Radoš and Vučetić, Dušan and Kiekens, Filip and Bugarski, Branko",
year = "2016",
abstract = "The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials – porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process – gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.",
journal = "Journal of Biotechnology",
title = "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts",
volume = "240",
doi = "10.1016/j.jbiotec.2016.10.017",
pages = "14-22"
}

Bukara, K., Drvenica, I., Ilić, V., Stančić, A., Mišić, D., Vasić, B., Gajić, R., Vučetić, D., Kiekens, F.,& Bugarski, B.. (2016). Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology, 240, 14-22.
https://doi.org/10.1016/j.jbiotec.2016.10.017

Bukara K, Drvenica I, Ilić V, Stančić A, Mišić D, Vasić B, Gajić R, Vučetić D, Kiekens F, Bugarski B. Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology. 2016;240:14-22.
doi:10.1016/j.jbiotec.2016.10.017 .

Bukara, Katarina, Drvenica, Ivana, Ilić, Vesna, Stančić, Ana, Mišić, Danijela, Vasić, Borislav, Gajić, Radoš, Vučetić, Dušan, Kiekens, Filip, Bugarski, Branko, "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts" in Journal of Biotechnology, 240 (2016):14-22,
https://doi.org/10.1016/j.jbiotec.2016.10.017 . .