TY  - DATA
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Jović, Jelena
AU  - Budečević, Sanja
AU  - Stojković, Biljana
AU  - Đorđević, Mirko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6696
AB  - Data sets for life history traits (early fecundity, total fecundity, lifespan and fertility) of female and male seed beetles (Acanthoscelides obtectus) harbouring one of the three naturally occurring mitochondrial haplotypes (MG1a, MG1d and MG3b).
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - TFT life history
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6696
ER  - 

@misc{
author = "Vlajnić, Lea and Savković, Uroš and Jović, Jelena and Budečević, Sanja and Stojković, Biljana and Đorđević, Mirko",
year = "2024",
abstract = "Data sets for life history traits (early fecundity, total fecundity, lifespan and fertility) of female and male seed beetles (Acanthoscelides obtectus) harbouring one of the three naturally occurring mitochondrial haplotypes (MG1a, MG1d and MG3b).",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "TFT life history",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6696"
}

Vlajnić, L., Savković, U., Jović, J., Budečević, S., Stojković, B.,& Đorđević, M.. (2024). TFT life history. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6696

Vlajnić L, Savković U, Jović J, Budečević S, Stojković B, Đorđević M. TFT life history. 2024;.
https://hdl.handle.net/21.15107/rcub_ibiss_6696 .

Vlajnić, Lea, Savković, Uroš, Jović, Jelena, Budečević, Sanja, Stojković, Biljana, Đorđević, Mirko, "TFT life history" (2024),
https://hdl.handle.net/21.15107/rcub_ibiss_6696 .

TY  - JOUR
AU  - Rončević, Aleksa
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Stojković, Biljana
AU  - Budečević, Sanja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6694
AB  - Morphological plasticity may be induced by altering host plants and commonly it is sexspecific in phytophagous insects. It is hypothesized that stress in insects caused by a host shift
leads to morphological changes and developmental destabilization, which may be identified
by fluctuating asymmetry. We performed reciprocal transplant experiments in eight replicated
populations of the seed beetle Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae,
Bruchinae) adapted to common bean (Phaseolus vulgaris L., Fabaceae) as its ancestral host
and chickpea (Cicer arietinum L., Fabaceae) as a suboptimal host. Using methods of
geometric morphometrics we assessed the effects of the short- and long-term host shift on size
and shape variation of beetles of both sexes. We also tested the hypothesis that fluctuating
asymmetry is lower when the beetles develop within seeds of the ancestral host. Our results
showed that females respond more plastically related to their body size and shape of the
abdomen, particularly during a short-term host shift, suggesting that females have a greater
importance in maintaining the population on a new host. The level of fluctuating asymmetry
is lowest in males that have evolved for generations on bean indicating that they have the
most canalized development, so we suggested that symmetry in A. obtectus is maintained by
sexual selection. Even if the beetles are adapted to a suboptimal host over many generations,
they stabilize their development after returning to the ancestral host. Our work indicates that
host shifts may change morphological aspects of the beetles in a sex-specific manner and
consequently influence their developmental trajectories.
PB  - John Wiley & Sons Ltd
T2  - Entomologia Experimentalis at Applicata
T1  - Sex-specific consequences of host shift for morphology and fluctuating asymmetry in a seed beetle: an experimental evolution approach
DO  - 10.1111/eea.13453
ER  - 

@article{
author = "Rončević, Aleksa and Savković, Uroš and Đorđević, Mirko and Vlajnić, Lea and Stojković, Biljana and Budečević, Sanja",
year = "2024",
abstract = "Morphological plasticity may be induced by altering host plants and commonly it is sexspecific in phytophagous insects. It is hypothesized that stress in insects caused by a host shift
leads to morphological changes and developmental destabilization, which may be identified
by fluctuating asymmetry. We performed reciprocal transplant experiments in eight replicated
populations of the seed beetle Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae,
Bruchinae) adapted to common bean (Phaseolus vulgaris L., Fabaceae) as its ancestral host
and chickpea (Cicer arietinum L., Fabaceae) as a suboptimal host. Using methods of
geometric morphometrics we assessed the effects of the short- and long-term host shift on size
and shape variation of beetles of both sexes. We also tested the hypothesis that fluctuating
asymmetry is lower when the beetles develop within seeds of the ancestral host. Our results
showed that females respond more plastically related to their body size and shape of the
abdomen, particularly during a short-term host shift, suggesting that females have a greater
importance in maintaining the population on a new host. The level of fluctuating asymmetry
is lowest in males that have evolved for generations on bean indicating that they have the
most canalized development, so we suggested that symmetry in A. obtectus is maintained by
sexual selection. Even if the beetles are adapted to a suboptimal host over many generations,
they stabilize their development after returning to the ancestral host. Our work indicates that
host shifts may change morphological aspects of the beetles in a sex-specific manner and
consequently influence their developmental trajectories.",
publisher = "John Wiley & Sons Ltd",
journal = "Entomologia Experimentalis at Applicata",
title = "Sex-specific consequences of host shift for morphology and fluctuating asymmetry in a seed beetle: an experimental evolution approach",
doi = "10.1111/eea.13453"
}

Rončević, A., Savković, U., Đorđević, M., Vlajnić, L., Stojković, B.,& Budečević, S.. (2024). Sex-specific consequences of host shift for morphology and fluctuating asymmetry in a seed beetle: an experimental evolution approach. in Entomologia Experimentalis at Applicata
John Wiley & Sons Ltd..
https://doi.org/10.1111/eea.13453

Rončević A, Savković U, Đorđević M, Vlajnić L, Stojković B, Budečević S. Sex-specific consequences of host shift for morphology and fluctuating asymmetry in a seed beetle: an experimental evolution approach. in Entomologia Experimentalis at Applicata. 2024;.
doi:10.1111/eea.13453 .

Rončević, Aleksa, Savković, Uroš, Đorđević, Mirko, Vlajnić, Lea, Stojković, Biljana, Budečević, Sanja, "Sex-specific consequences of host shift for morphology and fluctuating asymmetry in a seed beetle: an experimental evolution approach" in Entomologia Experimentalis at Applicata (2024),
https://doi.org/10.1111/eea.13453 . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Budečević, Sanja
AU  - Ljubisavljević, Katarina
AU  - Tomašević Kolarov, Nataša
AU  - Ajduković, Maja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6571
AB  - The modular organization of tetrapod paired limbs and girdles, influenced by the expression of Hox genes is one of the primary driving forces of the evolution of animal locomotion. The increased morphological diversification of the paired limbs is correlated with reduced between-limb covariation, while correlation within the elements is usually higher than between the elements. The tailed amphibians, such as Lissotriton newts, have a biphasic lifestyle with aquatic and terrestrial environments imposing different constraints on limb skeleton. By employing the methods of computerized microtomography and 3D geometric morphometrics, we explored the pattern of morphological variation, disparity, modularity and morphological integration in the proximal parts of the anterior limbs of six species of Eurasian small bodied newts. Although the species significantly differ in limb shape, there is a great overlap in morphology of scapula and humerus, and there are no differences in morphological disparity. For the scapula, the shape differences related to the duration of the aquatic period are in length, depth and curvature. The shape of the humerus is not affected by the length of aquatic period, and shape differences between the species are related to robustness of the body. The length of aquatic period has statistically supported phylogenetic signal. The scapula and humerus are structures of varying modularity. For the humerus, the strongest support on the phylogenetic level was for the capitulum/shaft hypothesis, which can also be interpreted as functional modularity. For the scapula, the greatest support was for the antero-posterior hypothesis of modularity in case of Lissotriton vulgaris, which can be explained by different functional roles and muscle insertion patterns, while there was no phylogenetic modularity. The modularity patterns seem to correspond with the general tetrapod pattern, with modularity being more pronounced in the distal structure. The future research should include more salamandrid taxa with different habitat preferences and both adult and larval stages, in order to explore how size, phylogeny and ecology affect the morphology and covariation patterns of limbs.
PB  - John Wiley and Sons
T2  - Journal of Anatomy
T1  - Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts
DO  - 10.1111/joa.14030
ER  - 

@article{
author = "Urošević, Aleksandar and Budečević, Sanja and Ljubisavljević, Katarina and Tomašević Kolarov, Nataša and Ajduković, Maja",
year = "2024",
abstract = "The modular organization of tetrapod paired limbs and girdles, influenced by the expression of Hox genes is one of the primary driving forces of the evolution of animal locomotion. The increased morphological diversification of the paired limbs is correlated with reduced between-limb covariation, while correlation within the elements is usually higher than between the elements. The tailed amphibians, such as Lissotriton newts, have a biphasic lifestyle with aquatic and terrestrial environments imposing different constraints on limb skeleton. By employing the methods of computerized microtomography and 3D geometric morphometrics, we explored the pattern of morphological variation, disparity, modularity and morphological integration in the proximal parts of the anterior limbs of six species of Eurasian small bodied newts. Although the species significantly differ in limb shape, there is a great overlap in morphology of scapula and humerus, and there are no differences in morphological disparity. For the scapula, the shape differences related to the duration of the aquatic period are in length, depth and curvature. The shape of the humerus is not affected by the length of aquatic period, and shape differences between the species are related to robustness of the body. The length of aquatic period has statistically supported phylogenetic signal. The scapula and humerus are structures of varying modularity. For the humerus, the strongest support on the phylogenetic level was for the capitulum/shaft hypothesis, which can also be interpreted as functional modularity. For the scapula, the greatest support was for the antero-posterior hypothesis of modularity in case of Lissotriton vulgaris, which can be explained by different functional roles and muscle insertion patterns, while there was no phylogenetic modularity. The modularity patterns seem to correspond with the general tetrapod pattern, with modularity being more pronounced in the distal structure. The future research should include more salamandrid taxa with different habitat preferences and both adult and larval stages, in order to explore how size, phylogeny and ecology affect the morphology and covariation patterns of limbs.",
publisher = "John Wiley and Sons",
journal = "Journal of Anatomy",
title = "Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts",
doi = "10.1111/joa.14030"
}

Urošević, A., Budečević, S., Ljubisavljević, K., Tomašević Kolarov, N.,& Ajduković, M.. (2024). Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts. in Journal of Anatomy
John Wiley and Sons..
https://doi.org/10.1111/joa.14030

Urošević A, Budečević S, Ljubisavljević K, Tomašević Kolarov N, Ajduković M. Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts. in Journal of Anatomy. 2024;.
doi:10.1111/joa.14030 .

Urošević, Aleksandar, Budečević, Sanja, Ljubisavljević, Katarina, Tomašević Kolarov, Nataša, Ajduković, Maja, "Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts" in Journal of Anatomy (2024),
https://doi.org/10.1111/joa.14030 . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Budečević, Sanja
AU  - Ljubisavljević, Katarina
AU  - Tomašević Kolarov, Nataša
AU  - Ajduković, Maja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6571
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6572
AB  - The modular organization of tetrapod paired limbs and girdles, influenced by the expression of Hox genes is one of the primary driving forces of the evolution of animal locomotion. The increased morphological diversification of the paired limbs is correlated with reduced between-limb covariation, while correlation within the elements is usually higher than between the elements. The tailed amphibians, such as Lissotriton newts, have a biphasic lifestyle with aquatic and terrestrial environments imposing different constraints on limb skeleton. By employing the methods of computerized microtomography and 3D geometric morphometrics, we explored the pattern of morphological variation, disparity, modularity and morphological integration in the proximal parts of the anterior limbs of six species of Eurasian small bodied newts. Although the species significantly differ in limb shape, there is a great overlap in morphology of scapula and humerus, and there are no differences in morphological disparity. For the scapula, the shape differences related to the duration of the aquatic period are in length, depth and curvature. The shape of the humerus is not affected by the length of aquatic period, and shape differences between the species are related to robustness of the body. The length of aquatic period has statistically supported phylogenetic signal. The scapula and humerus are structures of varying modularity. For the humerus, the strongest support on the phylogenetic level was for the capitulum/shaft hypothesis, which can also be interpreted as functional modularity. For the scapula, the greatest support was for the antero-posterior hypothesis of modularity in case of Lissotriton vulgaris, which can be explained by different functional roles and muscle insertion patterns, while there was no phylogenetic modularity. The modularity patterns seem to correspond with the general tetrapod pattern, with modularity being more pronounced in the distal structure. The future research should include more salamandrid taxa with different habitat preferences and both adult and larval stages, in order to explore how size, phylogeny and ecology affect the morphology and covariation patterns of limbs.
PB  - John Wiley and Sons
T2  - Journal of Anatomy
T1  - Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts
DO  - 10.1111/joa.14030
ER  - 

@article{
author = "Urošević, Aleksandar and Budečević, Sanja and Ljubisavljević, Katarina and Tomašević Kolarov, Nataša and Ajduković, Maja",
year = "2024",
abstract = "The modular organization of tetrapod paired limbs and girdles, influenced by the expression of Hox genes is one of the primary driving forces of the evolution of animal locomotion. The increased morphological diversification of the paired limbs is correlated with reduced between-limb covariation, while correlation within the elements is usually higher than between the elements. The tailed amphibians, such as Lissotriton newts, have a biphasic lifestyle with aquatic and terrestrial environments imposing different constraints on limb skeleton. By employing the methods of computerized microtomography and 3D geometric morphometrics, we explored the pattern of morphological variation, disparity, modularity and morphological integration in the proximal parts of the anterior limbs of six species of Eurasian small bodied newts. Although the species significantly differ in limb shape, there is a great overlap in morphology of scapula and humerus, and there are no differences in morphological disparity. For the scapula, the shape differences related to the duration of the aquatic period are in length, depth and curvature. The shape of the humerus is not affected by the length of aquatic period, and shape differences between the species are related to robustness of the body. The length of aquatic period has statistically supported phylogenetic signal. The scapula and humerus are structures of varying modularity. For the humerus, the strongest support on the phylogenetic level was for the capitulum/shaft hypothesis, which can also be interpreted as functional modularity. For the scapula, the greatest support was for the antero-posterior hypothesis of modularity in case of Lissotriton vulgaris, which can be explained by different functional roles and muscle insertion patterns, while there was no phylogenetic modularity. The modularity patterns seem to correspond with the general tetrapod pattern, with modularity being more pronounced in the distal structure. The future research should include more salamandrid taxa with different habitat preferences and both adult and larval stages, in order to explore how size, phylogeny and ecology affect the morphology and covariation patterns of limbs.",
publisher = "John Wiley and Sons",
journal = "Journal of Anatomy",
title = "Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts",
doi = "10.1111/joa.14030"
}

Urošević, A., Budečević, S., Ljubisavljević, K., Tomašević Kolarov, N.,& Ajduković, M.. (2024). Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts. in Journal of Anatomy
John Wiley and Sons..
https://doi.org/10.1111/joa.14030

Urošević A, Budečević S, Ljubisavljević K, Tomašević Kolarov N, Ajduković M. Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts. in Journal of Anatomy. 2024;.
doi:10.1111/joa.14030 .

Urošević, Aleksandar, Budečević, Sanja, Ljubisavljević, Katarina, Tomašević Kolarov, Nataša, Ajduković, Maja, "Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts" in Journal of Anatomy (2024),
https://doi.org/10.1111/joa.14030 . .

TY  - DATA
AU  - Budečević, Sanja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5873
AB  - This file contains data for the morphological analyses (landmarks coordinates) of reciprocal transplant laboratory evolution  experiment on seed beetles adapted to different plant hosts. This file is in the .csv format and it contains one sheet and fifty columns.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Data set for Sex-specific consequences of experimentally induced host shift on morphology and fluctuating asymmetry in seed beetle: experimental evolution approach
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5873
ER  - 

@misc{
author = "Budečević, Sanja",
year = "2023",
abstract = "This file contains data for the morphological analyses (landmarks coordinates) of reciprocal transplant laboratory evolution  experiment on seed beetles adapted to different plant hosts. This file is in the .csv format and it contains one sheet and fifty columns.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Data set for Sex-specific consequences of experimentally induced host shift on morphology and fluctuating asymmetry in seed beetle: experimental evolution approach",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5873"
}

Budečević, S.. (2023). Data set for Sex-specific consequences of experimentally induced host shift on morphology and fluctuating asymmetry in seed beetle: experimental evolution approach. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5873

Budečević S. Data set for Sex-specific consequences of experimentally induced host shift on morphology and fluctuating asymmetry in seed beetle: experimental evolution approach. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5873 .

Budečević, Sanja, "Data set for Sex-specific consequences of experimentally induced host shift on morphology and fluctuating asymmetry in seed beetle: experimental evolution approach" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5873 .

TY  - CONF
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Pavlović, Kasja
AU  - Krako Jakoviljević, Nina
AU  - Ivanović, Tijana
AU  - Budečević, Sanja
AU  - Stojković, Biljana
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Stojković, Oliver
AU  - Đorđević, Mirko
PY  - 2023
UR  - https://ece2023.com/scientific-program/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6252
AB  - Evolutionary theory suggests that inheritance of mitochondria through the maternal lineage could drive the accumulation of male-harming mutations in mitochondrial genomes. Namely, due to uniparental mode of mitochondrial inheritance, natural selection is “blind” to mutations that are deleterious to males as long as they are beneficial or neutral to females. This evolutionary phenomenon is known as ‘the Mother’s Curse’. The male-specific adverse effects of mitochondrial mutations are thought to be due to the fact that males are sensitive to impairments in the function of the oxidative phosphorylation (OXPHOS) system, i.e. mitochondrial respiration. The OXPHOS system consists of five complexes whose subunits are coded by the mitochondrial and nuclear genomes. In our previous work, we identified several mitochondrial haplotypes (mitotypes) in laboratory populations of the seed beetle (Acanthoscelides obtectus). One of them, MG3b mitotype, significantly reduces male fertility compared to other mitotypes when expressed in the same nuclear environment, but has no negative effects on females. In this study, we tested whether the MG3b mitotype affects OXPHOS functioning in males compared with other, control mitotypes. Specifically, we expressed MG3b and two control mitotypes alongside the same nuclear background and measured mitochondrial respiration in males using high-resolution respirometry. Our results show that respiration linked to all OXPHOS complexes is significantly reduced in MG3b males compared with controls. This is particularly true for the complex IV, which shows mean reduction in activity of around 40%. This work provides evidence that the decrease in mitochondrial respiration is the cause of subfertility of MG3b males.
PB  - Hellenic Entomological Society
C3  - Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
T1  - Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility
SP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6252
ER  - 

@conference{
author = "Vlajnić, Lea and Savković, Uroš and Pavlović, Kasja and Krako Jakoviljević, Nina and Ivanović, Tijana and Budečević, Sanja and Stojković, Biljana and Pešić, Snežana and Vukajlović, Filip and Predojević, Dragana and Mitrovski Bogdanović, Ana and Stojković, Oliver and Đorđević, Mirko",
year = "2023",
abstract = "Evolutionary theory suggests that inheritance of mitochondria through the maternal lineage could drive the accumulation of male-harming mutations in mitochondrial genomes. Namely, due to uniparental mode of mitochondrial inheritance, natural selection is “blind” to mutations that are deleterious to males as long as they are beneficial or neutral to females. This evolutionary phenomenon is known as ‘the Mother’s Curse’. The male-specific adverse effects of mitochondrial mutations are thought to be due to the fact that males are sensitive to impairments in the function of the oxidative phosphorylation (OXPHOS) system, i.e. mitochondrial respiration. The OXPHOS system consists of five complexes whose subunits are coded by the mitochondrial and nuclear genomes. In our previous work, we identified several mitochondrial haplotypes (mitotypes) in laboratory populations of the seed beetle (Acanthoscelides obtectus). One of them, MG3b mitotype, significantly reduces male fertility compared to other mitotypes when expressed in the same nuclear environment, but has no negative effects on females. In this study, we tested whether the MG3b mitotype affects OXPHOS functioning in males compared with other, control mitotypes. Specifically, we expressed MG3b and two control mitotypes alongside the same nuclear background and measured mitochondrial respiration in males using high-resolution respirometry. Our results show that respiration linked to all OXPHOS complexes is significantly reduced in MG3b males compared with controls. This is particularly true for the complex IV, which shows mean reduction in activity of around 40%. This work provides evidence that the decrease in mitochondrial respiration is the cause of subfertility of MG3b males.",
publisher = "Hellenic Entomological Society",
journal = "Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece",
title = "Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility",
pages = "40",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6252"
}

Vlajnić, L., Savković, U., Pavlović, K., Krako Jakoviljević, N., Ivanović, T., Budečević, S., Stojković, B., Pešić, S., Vukajlović, F., Predojević, D., Mitrovski Bogdanović, A., Stojković, O.,& Đorđević, M.. (2023). Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
Hellenic Entomological Society., 40.
https://hdl.handle.net/21.15107/rcub_ibiss_6252

Vlajnić L, Savković U, Pavlović K, Krako Jakoviljević N, Ivanović T, Budečević S, Stojković B, Pešić S, Vukajlović F, Predojević D, Mitrovski Bogdanović A, Stojković O, Đorđević M. Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece. 2023;:40.
https://hdl.handle.net/21.15107/rcub_ibiss_6252 .

Vlajnić, Lea, Savković, Uroš, Pavlović, Kasja, Krako Jakoviljević, Nina, Ivanović, Tijana, Budečević, Sanja, Stojković, Biljana, Pešić, Snežana, Vukajlović, Filip, Predojević, Dragana, Mitrovski Bogdanović, Ana, Stojković, Oliver, Đorđević, Mirko, "Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility" in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece (2023):40,
https://hdl.handle.net/21.15107/rcub_ibiss_6252 .

TY  - CONF
AU  - Budečević, Sanja
AU  - Rončević, Aleksa
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Stojković, Biljana
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Stojković, Oliver
AU  - Savković, Uroš
PY  - 2023
UR  - https://ece2023.com/scientific-program/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6254
AB  - Host-shift induced stress in phytophagous insects could have effect on developmental destabilization. Indicator of developmental instability is fluctuating asymmetry (FA)- small random deviations between right and left side of bilateral symmetrical traits. In theory, symmetry is subject to sexual selection in insects, since FA is considered as an indicator of male quality. Response to environmental variation is commonly sex-specific and can affect patterns of sexual dimorphism. We performed reciprocal transplant experiment in eight replicated seed beetle (Acanthoscelides obtectus) populations adapted to bean and chickpea. Within the framework of experimental evolution and using the method of geometric morphometrics we: 1. observed the effects of long and short term host-shift on patterns of shape variation of beetles of both sexes and 2. tested hypothesis that both sexes will have a higher level of FA after long or short term change from optimal to suboptimal host. We found that long term host-shift process affected shape in a way that bean adapted beetles had wider and shorter abdomen compared to chickpea adapted ones. Short-term effects are more notable in bean selected beetles, especially in the abdominal region. Bean adapted males had the most canalized development, i.e. the lowest level of FA, compared to chickpea beetles. In chickpea adapted females, difference in level of FA significantly decreased after the short term host-shift. Our work indicates that host-shift process can change morphological aspects of beetles in a sex-specific manner and consequently influence their developmental trajectories.
PB  - Hellenic Entomological Society
C3  - Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
T1  - Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach
SP  - 362
EP  - 363
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6254
ER  - 

@conference{
author = "Budečević, Sanja and Rončević, Aleksa and Đorđević, Mirko and Vlajnić, Lea and Stojković, Biljana and Pešić, Snežana and Vukajlović, Filip and Predojević, Dragana and Mitrovski Bogdanović, Ana and Stojković, Oliver and Savković, Uroš",
year = "2023",
abstract = "Host-shift induced stress in phytophagous insects could have effect on developmental destabilization. Indicator of developmental instability is fluctuating asymmetry (FA)- small random deviations between right and left side of bilateral symmetrical traits. In theory, symmetry is subject to sexual selection in insects, since FA is considered as an indicator of male quality. Response to environmental variation is commonly sex-specific and can affect patterns of sexual dimorphism. We performed reciprocal transplant experiment in eight replicated seed beetle (Acanthoscelides obtectus) populations adapted to bean and chickpea. Within the framework of experimental evolution and using the method of geometric morphometrics we: 1. observed the effects of long and short term host-shift on patterns of shape variation of beetles of both sexes and 2. tested hypothesis that both sexes will have a higher level of FA after long or short term change from optimal to suboptimal host. We found that long term host-shift process affected shape in a way that bean adapted beetles had wider and shorter abdomen compared to chickpea adapted ones. Short-term effects are more notable in bean selected beetles, especially in the abdominal region. Bean adapted males had the most canalized development, i.e. the lowest level of FA, compared to chickpea beetles. In chickpea adapted females, difference in level of FA significantly decreased after the short term host-shift. Our work indicates that host-shift process can change morphological aspects of beetles in a sex-specific manner and consequently influence their developmental trajectories.",
publisher = "Hellenic Entomological Society",
journal = "Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece",
title = "Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach",
pages = "362-363",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6254"
}

Budečević, S., Rončević, A., Đorđević, M., Vlajnić, L., Stojković, B., Pešić, S., Vukajlović, F., Predojević, D., Mitrovski Bogdanović, A., Stojković, O.,& Savković, U.. (2023). Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
Hellenic Entomological Society., 362-363.
https://hdl.handle.net/21.15107/rcub_ibiss_6254

Budečević S, Rončević A, Đorđević M, Vlajnić L, Stojković B, Pešić S, Vukajlović F, Predojević D, Mitrovski Bogdanović A, Stojković O, Savković U. Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece. 2023;:362-363.
https://hdl.handle.net/21.15107/rcub_ibiss_6254 .

Budečević, Sanja, Rončević, Aleksa, Đorđević, Mirko, Vlajnić, Lea, Stojković, Biljana, Pešić, Snežana, Vukajlović, Filip, Predojević, Dragana, Mitrovski Bogdanović, Ana, Stojković, Oliver, Savković, Uroš, "Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach" in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece (2023):362-363,
https://hdl.handle.net/21.15107/rcub_ibiss_6254 .

TY  - CONF
AU  - Predojević, Dragana
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Stojković, Biljana
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Mitrovski Bogdanović, Ana
AU  - Stojković, Oliver
AU  - Budečević, Sanja
PY  - 2023
UR  - https://ece2023.com/scientific-program/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6253
AB  - Insecticide resistance is an example of rapid evolution under strong selection pressure and, is therefore of great importance for human and environmental health and economy. Acanthoscelides obtectus is a globally distributed Coleoptera pest that attacks bean seeds and degrades their quality. One method of protecting against seed beetles is the application of a widely used group of pesticides- pyrethroids. Insecticides, as xenobiotics, can induce stress in pests, leading to destabilization of their development and changes in their morphology. Fluctuating asymmetry (FA), small random deviations between right and left sides of bilateral symmetrical traits, is considered as a result of developmental instability and is thus a measurable indicator of phenotypic response to stress during morphogenesis in insects. In this work, we exposed four populations of A. obtectus to sub-lethal doses (LD20) of pyrethroid insecticide for five generations in a laboratory evolution experiment. To estimate the level of stress, we monitored the effects of the pesticide on shape changes and the level of FA using a geometric morphometric approach. Our results showed that beetles treated with LD20 significantly changed their morphology in the thorax region, while the effect on the level of FA was minor. This work uses experimental evolution as a powerful tool to study pesticide resistance and paves the way for further studies on morphological adaptations, modularity and developmental stability under stress conditions.
PB  - Hellenic Entomological Society
C3  - Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
T1  - Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development
SP  - 365
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6253
ER  - 

@conference{
author = "Predojević, Dragana and Savković, Uroš and Đorđević, Mirko and Vlajnić, Lea and Stojković, Biljana and Pešić, Snežana and Vukajlović, Filip and Mitrovski Bogdanović, Ana and Stojković, Oliver and Budečević, Sanja",
year = "2023",
abstract = "Insecticide resistance is an example of rapid evolution under strong selection pressure and, is therefore of great importance for human and environmental health and economy. Acanthoscelides obtectus is a globally distributed Coleoptera pest that attacks bean seeds and degrades their quality. One method of protecting against seed beetles is the application of a widely used group of pesticides- pyrethroids. Insecticides, as xenobiotics, can induce stress in pests, leading to destabilization of their development and changes in their morphology. Fluctuating asymmetry (FA), small random deviations between right and left sides of bilateral symmetrical traits, is considered as a result of developmental instability and is thus a measurable indicator of phenotypic response to stress during morphogenesis in insects. In this work, we exposed four populations of A. obtectus to sub-lethal doses (LD20) of pyrethroid insecticide for five generations in a laboratory evolution experiment. To estimate the level of stress, we monitored the effects of the pesticide on shape changes and the level of FA using a geometric morphometric approach. Our results showed that beetles treated with LD20 significantly changed their morphology in the thorax region, while the effect on the level of FA was minor. This work uses experimental evolution as a powerful tool to study pesticide resistance and paves the way for further studies on morphological adaptations, modularity and developmental stability under stress conditions.",
publisher = "Hellenic Entomological Society",
journal = "Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece",
title = "Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development",
pages = "365",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6253"
}

Predojević, D., Savković, U., Đorđević, M., Vlajnić, L., Stojković, B., Pešić, S., Vukajlović, F., Mitrovski Bogdanović, A., Stojković, O.,& Budečević, S.. (2023). Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
Hellenic Entomological Society., 365.
https://hdl.handle.net/21.15107/rcub_ibiss_6253

Predojević D, Savković U, Đorđević M, Vlajnić L, Stojković B, Pešić S, Vukajlović F, Mitrovski Bogdanović A, Stojković O, Budečević S. Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece. 2023;:365.
https://hdl.handle.net/21.15107/rcub_ibiss_6253 .

Predojević, Dragana, Savković, Uroš, Đorđević, Mirko, Vlajnić, Lea, Stojković, Biljana, Pešić, Snežana, Vukajlović, Filip, Mitrovski Bogdanović, Ana, Stojković, Oliver, Budečević, Sanja, "Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development" in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece (2023):365,
https://hdl.handle.net/21.15107/rcub_ibiss_6253 .

TY  - DATA
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Vukajlović, Filip
AU  - Budečević, Sanja
AU  - Stojković, Biljana
AU  - Stojković, Oliver
AU  - Pešić, Snežana
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Đorđević, Mirko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6017
AB  - This data set is a milestone  of the WP3 work package of the Experimental evolution approach in developing insect pest control methods (ELEVATE) project number #7683961 financed by the Science Fund of the Republic of Serbia. Data set contains raw data on the effects of candidate Trojan female technique (TFT) mutations across the range of nuclear backgrounds on fertility of male and female seed beetles. For obtaining this data set we have used controlled genetic crosses between females with 3 specific mitotypes (1 of which is a candidate TFT mitotype, and 2 are controls) and males with 5 different nuclear backgrounds. Such crossings were used to assess female and male fertility of seed beetles with different combinations of mito-nuclear genomes.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6017
ER  - 

@misc{
author = "Vlajnić, Lea and Savković, Uroš and Vukajlović, Filip and Budečević, Sanja and Stojković, Biljana and Stojković, Oliver and Pešić, Snežana and Predojević, Dragana and Mitrovski Bogdanović, Ana and Đorđević, Mirko",
year = "2023",
abstract = "This data set is a milestone  of the WP3 work package of the Experimental evolution approach in developing insect pest control methods (ELEVATE) project number #7683961 financed by the Science Fund of the Republic of Serbia. Data set contains raw data on the effects of candidate Trojan female technique (TFT) mutations across the range of nuclear backgrounds on fertility of male and female seed beetles. For obtaining this data set we have used controlled genetic crosses between females with 3 specific mitotypes (1 of which is a candidate TFT mitotype, and 2 are controls) and males with 5 different nuclear backgrounds. Such crossings were used to assess female and male fertility of seed beetles with different combinations of mito-nuclear genomes.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6017"
}

Vlajnić, L., Savković, U., Vukajlović, F., Budečević, S., Stojković, B., Stojković, O., Pešić, S., Predojević, D., Mitrovski Bogdanović, A.,& Đorđević, M.. (2023). Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6017

Vlajnić L, Savković U, Vukajlović F, Budečević S, Stojković B, Stojković O, Pešić S, Predojević D, Mitrovski Bogdanović A, Đorđević M. Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6017 .

Vlajnić, Lea, Savković, Uroš, Vukajlović, Filip, Budečević, Sanja, Stojković, Biljana, Stojković, Oliver, Pešić, Snežana, Predojević, Dragana, Mitrovski Bogdanović, Ana, Đorđević, Mirko, "Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6017 .

TY  - DATA
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Vukajlović, Filip
AU  - Budečević, Sanja
AU  - Stojković, Biljana
AU  - Stojković, Oliver
AU  - Pešić, Snežana
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Đorđević, Mirko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6018
AB  - This data set is a milestone of the WP3 work package of the Experimental evolution approach in developing insect pest control methods (ELEVATE) project number #7683961 financed by the Science Fund of the Republic of Serbia. Data set contains sequences of subunit 1 of cytochrome oxidase (COI) for populations of seed beetles (Acanthoscelides obtectus) with different shares of Trojan female technique (TFT) and control mytotypes.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6018
ER  - 

@misc{
author = "Vlajnić, Lea and Savković, Uroš and Vukajlović, Filip and Budečević, Sanja and Stojković, Biljana and Stojković, Oliver and Pešić, Snežana and Predojević, Dragana and Mitrovski Bogdanović, Ana and Đorđević, Mirko",
year = "2023",
abstract = "This data set is a milestone of the WP3 work package of the Experimental evolution approach in developing insect pest control methods (ELEVATE) project number #7683961 financed by the Science Fund of the Republic of Serbia. Data set contains sequences of subunit 1 of cytochrome oxidase (COI) for populations of seed beetles (Acanthoscelides obtectus) with different shares of Trojan female technique (TFT) and control mytotypes.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6018"
}

Vlajnić, L., Savković, U., Vukajlović, F., Budečević, S., Stojković, B., Stojković, O., Pešić, S., Predojević, D., Mitrovski Bogdanović, A.,& Đorđević, M.. (2023). Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6018

Vlajnić L, Savković U, Vukajlović F, Budečević S, Stojković B, Stojković O, Pešić S, Predojević D, Mitrovski Bogdanović A, Đorđević M. Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6018 .

Vlajnić, Lea, Savković, Uroš, Vukajlović, Filip, Budečević, Sanja, Stojković, Biljana, Stojković, Oliver, Pešić, Snežana, Predojević, Dragana, Mitrovski Bogdanović, Ana, Đorđević, Mirko, "Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6018 .

TY  - JOUR
AU  - Zlatić, Nenad
AU  - Budečević, Sanja
AU  - Stanković, Milan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5891
AB  - The shape–environment relationship in plants refers to the ways in which the physical
characteristics and structures of plants are influenced by their environment. Plants have evolved a
remarkable ability to adapt to their specific habitats, and their shape and form play a crucial role
in determining their survival and reproductive success. This study aimed to examine differences
in size and shape between morphological traits in mountain germander (Teucrium montanum L.)
from different geological substrates (calcareous and serpentinite). For this study, 400 individuals of
T. montanum from 20 populations (ten populations from the serpentinite and ten from the calcareous
substrate) were selected. Using the geometric morphometrics approach, it was shown that the degree
of phenotypic variation in the size and shape of the corolla, leaf, and stem of T. montanum depends
on the type of substrate. The main differences between the populations are the narrower part of the
lower lip of the corolla, the narrower leaf, and the wider central part of the vascular system stem
from serpentinite populations. The results of this study will contribute to a better understanding
of the morphological variability of T. montanum in relation to edaphic conditions. In addition, the
results confirm that certain morphological differences play an important role in the adaptive response
in relation to substrate composition, especially for substrates with increased metal content, such as
serpentinite. The shape–environment relationship in plants could define diversity and complexity in
plant life, and underscores the importance of shape as a key factor in their survival and success in
different habitats.
PB  - Basel: MDPI
T2  - Plants
T1  - Geological Substrate Effects on Teucrium montanum L. (Lamiaceae) Morphological Traits: Geometric Morphometrics Approach
IS  - 12
VL  - 12
DO  - 10.3390/plants12122381
SP  - 2381
ER  - 

@article{
author = "Zlatić, Nenad and Budečević, Sanja and Stanković, Milan",
year = "2023",
abstract = "The shape–environment relationship in plants refers to the ways in which the physical
characteristics and structures of plants are influenced by their environment. Plants have evolved a
remarkable ability to adapt to their specific habitats, and their shape and form play a crucial role
in determining their survival and reproductive success. This study aimed to examine differences
in size and shape between morphological traits in mountain germander (Teucrium montanum L.)
from different geological substrates (calcareous and serpentinite). For this study, 400 individuals of
T. montanum from 20 populations (ten populations from the serpentinite and ten from the calcareous
substrate) were selected. Using the geometric morphometrics approach, it was shown that the degree
of phenotypic variation in the size and shape of the corolla, leaf, and stem of T. montanum depends
on the type of substrate. The main differences between the populations are the narrower part of the
lower lip of the corolla, the narrower leaf, and the wider central part of the vascular system stem
from serpentinite populations. The results of this study will contribute to a better understanding
of the morphological variability of T. montanum in relation to edaphic conditions. In addition, the
results confirm that certain morphological differences play an important role in the adaptive response
in relation to substrate composition, especially for substrates with increased metal content, such as
serpentinite. The shape–environment relationship in plants could define diversity and complexity in
plant life, and underscores the importance of shape as a key factor in their survival and success in
different habitats.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Geological Substrate Effects on Teucrium montanum L. (Lamiaceae) Morphological Traits: Geometric Morphometrics Approach",
number = "12",
volume = "12",
doi = "10.3390/plants12122381",
pages = "2381"
}

Zlatić, N., Budečević, S.,& Stanković, M.. (2023). Geological Substrate Effects on Teucrium montanum L. (Lamiaceae) Morphological Traits: Geometric Morphometrics Approach. in Plants
Basel: MDPI., 12(12), 2381.
https://doi.org/10.3390/plants12122381

Zlatić N, Budečević S, Stanković M. Geological Substrate Effects on Teucrium montanum L. (Lamiaceae) Morphological Traits: Geometric Morphometrics Approach. in Plants. 2023;12(12):2381.
doi:10.3390/plants12122381 .

Zlatić, Nenad, Budečević, Sanja, Stanković, Milan, "Geological Substrate Effects on Teucrium montanum L. (Lamiaceae) Morphological Traits: Geometric Morphometrics Approach" in Plants, 12, no. 12 (2023):2381,
https://doi.org/10.3390/plants12122381 . .

TY  - JOUR
AU  - Budečević, Sanja
AU  - Hočevar, Katarina
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5857
AB  - To gain insight into the evolution of flower traits in the generalized food-deceptive plant Iris pumila, we assessed the color, size, shape, and fluctuating asymmetry (FA) of three functionally distinct floral organs—outer perianths (‘falls’), inner perianths (‘standards’), and style branches—and estimated pollinator-mediated selection on these traits. We evaluated the perianth color as the achromatic brightness of the fall, measured the flower stem height, and analyzed the floral organ size, shape, and FA using geometric morphometrics. Pollinated flowers had significantly higher brightness, longer flower stems, and larger floral organs compared to non-pollinated flowers. The shape and FA of the floral organs did not differ, except for the fall FA, where higher values were found for falls of pollinated flowers. Pollinator-mediated selection was confirmed for flower stem height and for subtle changes in the shape of the fall and style branch—organs that form the pollination tunnel. This study provides evidence that, although all analyzed flower traits play significant roles in pollinator attraction, flower stem height and pollination tunnel shape evolved under the pollinator-mediated selection, whereas achromatic brightness, size, and symmetry of floral organs did not directly affect pollination success.
PB  - Basel : MDPI
T2  - Symmetry
T1  - Phenotypic Selection on Flower Traits in Food-Deceptive Plant Iris pumila L.: The Role of Pollinators
IS  - 6
VL  - 15
DO  - 10.3390/sym15061149
SP  - 1149
ER  - 

@article{
author = "Budečević, Sanja and Hočevar, Katarina and Manitašević Jovanović, Sanja and Vuleta, Ana",
year = "2023",
abstract = "To gain insight into the evolution of flower traits in the generalized food-deceptive plant Iris pumila, we assessed the color, size, shape, and fluctuating asymmetry (FA) of three functionally distinct floral organs—outer perianths (‘falls’), inner perianths (‘standards’), and style branches—and estimated pollinator-mediated selection on these traits. We evaluated the perianth color as the achromatic brightness of the fall, measured the flower stem height, and analyzed the floral organ size, shape, and FA using geometric morphometrics. Pollinated flowers had significantly higher brightness, longer flower stems, and larger floral organs compared to non-pollinated flowers. The shape and FA of the floral organs did not differ, except for the fall FA, where higher values were found for falls of pollinated flowers. Pollinator-mediated selection was confirmed for flower stem height and for subtle changes in the shape of the fall and style branch—organs that form the pollination tunnel. This study provides evidence that, although all analyzed flower traits play significant roles in pollinator attraction, flower stem height and pollination tunnel shape evolved under the pollinator-mediated selection, whereas achromatic brightness, size, and symmetry of floral organs did not directly affect pollination success.",
publisher = "Basel : MDPI",
journal = "Symmetry",
title = "Phenotypic Selection on Flower Traits in Food-Deceptive Plant Iris pumila L.: The Role of Pollinators",
number = "6",
volume = "15",
doi = "10.3390/sym15061149",
pages = "1149"
}

Budečević, S., Hočevar, K., Manitašević Jovanović, S.,& Vuleta, A.. (2023). Phenotypic Selection on Flower Traits in Food-Deceptive Plant Iris pumila L.: The Role of Pollinators. in Symmetry
Basel : MDPI., 15(6), 1149.
https://doi.org/10.3390/sym15061149

Budečević S, Hočevar K, Manitašević Jovanović S, Vuleta A. Phenotypic Selection on Flower Traits in Food-Deceptive Plant Iris pumila L.: The Role of Pollinators. in Symmetry. 2023;15(6):1149.
doi:10.3390/sym15061149 .

Budečević, Sanja, Hočevar, Katarina, Manitašević Jovanović, Sanja, Vuleta, Ana, "Phenotypic Selection on Flower Traits in Food-Deceptive Plant Iris pumila L.: The Role of Pollinators" in Symmetry, 15, no. 6 (2023):1149,
https://doi.org/10.3390/sym15061149 . .

TY  - JOUR
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Budečević, Sanja
AU  - Stojković, Biljana
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1111/een.13222
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5352
AB  - Expansion of the host range in phytophagous insects, followed by the specialisation on novel hosts, encompasses changes in many aspects of insects' behaviour, physiology, and the interaction between their life-history features. Here, we analyse the roles of insects' developmental plasticity in the process of host shift. Using laboratory populations of the seed beetle (Acanthoscelides obtectus), which have evolved on both optimal (common beans) and suboptimal (chickpea) plant hosts for more than 35 years, we experimentally replicated the process of host shift and analysed the patterns of short-term and long-term life-history responses to host variation. In order to test whether selection for increased plasticity has an effect on host shifting processes, we used existing bean and chickpea adapted populations to establish new populations in which the host plant offered for insect development was changed each generation (for 13 generations). To test the potential for a short-term plastic response, beetles from each laboratory population were raised on both hosts for one generation. Results showed that, in contrast to the populations that evolved on beans, which maintained high levels of developmental plasticity, long-term host switching to chickpeas was accompanied with specialisation of pre-adult viability with a simultaneous increase in fecundity. Populations evolved on alternate plant hosts that revealed similar plasticity patterns as their ancestral populations. These results suggest that short-term plastic responses could determine the paths of long-term evolution of life-history plasticity. However, more time could be needed for plasticity to evolve differently from the initial responses.
T2  - Ecological Entomology
T1  - Evolution of developmental plasticity and the potential of host shift in the seed beetle: Insights from laboratory evolution experiments
DO  - 10.1111/een.13222
ER  - 

@article{
author = "Savković, Uroš and Đorđević, Mirko and Vlajnić, Lea and Budečević, Sanja and Stojković, Biljana",
year = "2022",
abstract = "Expansion of the host range in phytophagous insects, followed by the specialisation on novel hosts, encompasses changes in many aspects of insects' behaviour, physiology, and the interaction between their life-history features. Here, we analyse the roles of insects' developmental plasticity in the process of host shift. Using laboratory populations of the seed beetle (Acanthoscelides obtectus), which have evolved on both optimal (common beans) and suboptimal (chickpea) plant hosts for more than 35 years, we experimentally replicated the process of host shift and analysed the patterns of short-term and long-term life-history responses to host variation. In order to test whether selection for increased plasticity has an effect on host shifting processes, we used existing bean and chickpea adapted populations to establish new populations in which the host plant offered for insect development was changed each generation (for 13 generations). To test the potential for a short-term plastic response, beetles from each laboratory population were raised on both hosts for one generation. Results showed that, in contrast to the populations that evolved on beans, which maintained high levels of developmental plasticity, long-term host switching to chickpeas was accompanied with specialisation of pre-adult viability with a simultaneous increase in fecundity. Populations evolved on alternate plant hosts that revealed similar plasticity patterns as their ancestral populations. These results suggest that short-term plastic responses could determine the paths of long-term evolution of life-history plasticity. However, more time could be needed for plasticity to evolve differently from the initial responses.",
journal = "Ecological Entomology",
title = "Evolution of developmental plasticity and the potential of host shift in the seed beetle: Insights from laboratory evolution experiments",
doi = "10.1111/een.13222"
}

Savković, U., Đorđević, M., Vlajnić, L., Budečević, S.,& Stojković, B.. (2022). Evolution of developmental plasticity and the potential of host shift in the seed beetle: Insights from laboratory evolution experiments. in Ecological Entomology.
https://doi.org/10.1111/een.13222

Savković U, Đorđević M, Vlajnić L, Budečević S, Stojković B. Evolution of developmental plasticity and the potential of host shift in the seed beetle: Insights from laboratory evolution experiments. in Ecological Entomology. 2022;.
doi:10.1111/een.13222 .

Savković, Uroš, Đorđević, Mirko, Vlajnić, Lea, Budečević, Sanja, Stojković, Biljana, "Evolution of developmental plasticity and the potential of host shift in the seed beetle: Insights from laboratory evolution experiments" in Ecological Entomology (2022),
https://doi.org/10.1111/een.13222 . .

TY  - JOUR
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
AU  - Tucić, Branka
AU  - Klingenberg, Christian Peter
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4997
AB  - Directional asymmetry is a systematic difference between the left and right sides for structures with bilateral symmetry or a systematic differentiation among repeated parts for complex symmetry. This study explores factors that produce directional asymmetry in the flower of Iris pumila, a structure with complex symmetry that makes it possible to investigate multiple such factors simultaneously. The shapes and sizes of three types of floral organs, the falls, standards, and style branches, were quantified using the methods of geometric morphometrics. For each flower, this study recorded the compass orientations of floral organs as well as their anatomical orientations relative to the two spathes subtending each flower. To characterize directional asymmetry at the whole-flower level, differences in the average sizes and shapes according to compass orientation and relative orientation were computed, and the left–right asymmetry was also evaluated for each individual organ. No size or shape differences within flowers were found in relation to anatomical position; this may relate to the terminal position of flowers in Iris pumila, suggesting that there may be no adaxial–abaxial polarity, which is very prominent in many other taxa. There was clear directional asymmetry of shape in relation to compass orientation, presumably driven by a consistent environmental gradient such as solar irradiance. There was also clear directional asymmetry between left and right halves of every floral organ, most likely related to the arrangement of organs in the bud. These findings indicate that different factors are acting to produce directional asymmetry at different levels. In conventional analyses not recording flower orientations, these effects would be impossible to disentangle from each other and would probably be included as part of fluctuating asymmetry.
PB  - Hoboken : John Wiley & Sons Ltd
T2  - Evolution & Development
T1  - Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry
DO  - 10.1111/ede.12402
ER  - 

@article{
author = "Budečević, Sanja and Manitašević Jovanović, Sanja and Vuleta, Ana and Tucić, Branka and Klingenberg, Christian Peter",
year = "2022",
abstract = "Directional asymmetry is a systematic difference between the left and right sides for structures with bilateral symmetry or a systematic differentiation among repeated parts for complex symmetry. This study explores factors that produce directional asymmetry in the flower of Iris pumila, a structure with complex symmetry that makes it possible to investigate multiple such factors simultaneously. The shapes and sizes of three types of floral organs, the falls, standards, and style branches, were quantified using the methods of geometric morphometrics. For each flower, this study recorded the compass orientations of floral organs as well as their anatomical orientations relative to the two spathes subtending each flower. To characterize directional asymmetry at the whole-flower level, differences in the average sizes and shapes according to compass orientation and relative orientation were computed, and the left–right asymmetry was also evaluated for each individual organ. No size or shape differences within flowers were found in relation to anatomical position; this may relate to the terminal position of flowers in Iris pumila, suggesting that there may be no adaxial–abaxial polarity, which is very prominent in many other taxa. There was clear directional asymmetry of shape in relation to compass orientation, presumably driven by a consistent environmental gradient such as solar irradiance. There was also clear directional asymmetry between left and right halves of every floral organ, most likely related to the arrangement of organs in the bud. These findings indicate that different factors are acting to produce directional asymmetry at different levels. In conventional analyses not recording flower orientations, these effects would be impossible to disentangle from each other and would probably be included as part of fluctuating asymmetry.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "Evolution & Development",
title = "Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry",
doi = "10.1111/ede.12402"
}

Budečević, S., Manitašević Jovanović, S., Vuleta, A., Tucić, B.,& Klingenberg, C. P.. (2022). Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry. in Evolution & Development
Hoboken : John Wiley & Sons Ltd..
https://doi.org/10.1111/ede.12402

Budečević S, Manitašević Jovanović S, Vuleta A, Tucić B, Klingenberg CP. Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry. in Evolution & Development. 2022;.
doi:10.1111/ede.12402 .

Budečević, Sanja, Manitašević Jovanović, Sanja, Vuleta, Ana, Tucić, Branka, Klingenberg, Christian Peter, "Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry" in Evolution & Development (2022),
https://doi.org/10.1111/ede.12402 . .

TY  - CONF
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Budečević, Sanja
AU  - Vlajnić, Lea
AU  - Stojković, Biljana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5034
AB  - Eksperimentalna (laboratorijska) evolucija predstavlja savremen metodološki pristup u empirijskom testiranju evoluciono-bioloških hipoteza. Stavljajući model sisteme u uslove koji su unapred definisani i konstantni, eksperimentalnom evolucijom mogu se pratiti transgeneracijske fenotipske i genetičke promene populacija što omogućava proučavanje procesa adaptacija. Pojedine eksperimentalne populacije pasuljevog žiška su pod specifičnim režimima laboratorijske evolucije više od 35 godina i predstavljaju jedinstven primer višedecenijske eksperimentalne evolucije na svetu. Ovo predavanje obuhvatiće primere koji ilustruju evoluciju fenotipske plastičnosti osobina životne istorije u uslovima izmenjene biljke domaćina uključujući promene u ponašanju prilikom polaganja jaja, morfološke promene i populacionu dinamiku. Dodatano biće predstavljeni rezultati populacija koje su održavane u režimima za ranu i kasnu reprodukciju koje su dovele do velikih promena na molekularnom, biohemijskom i fiziološkom nivou. Konačno, posebna pažnja će biti posvećena primeni eksperimentalne evolucije u razvoju savremene metode kontrole brojnosti insekata koja se zasniva na specifičnim mitohondrijskim mutacijama s polno specifičnim efektima koji ograničavaju reproduktivni potencijal mužjaka bez negativnih efekata na ženke (tehnika trojanskih ženki).
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Aktualne i višedecenijske studije eksperimentalne evolucije na pasuljevom žišku
SP  - 93
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5034
ER  - 

@conference{
author = "Savković, Uroš and Đorđević, Mirko and Budečević, Sanja and Vlajnić, Lea and Stojković, Biljana",
year = "2022",
abstract = "Eksperimentalna (laboratorijska) evolucija predstavlja savremen metodološki pristup u empirijskom testiranju evoluciono-bioloških hipoteza. Stavljajući model sisteme u uslove koji su unapred definisani i konstantni, eksperimentalnom evolucijom mogu se pratiti transgeneracijske fenotipske i genetičke promene populacija što omogućava proučavanje procesa adaptacija. Pojedine eksperimentalne populacije pasuljevog žiška su pod specifičnim režimima laboratorijske evolucije više od 35 godina i predstavljaju jedinstven primer višedecenijske eksperimentalne evolucije na svetu. Ovo predavanje obuhvatiće primere koji ilustruju evoluciju fenotipske plastičnosti osobina životne istorije u uslovima izmenjene biljke domaćina uključujući promene u ponašanju prilikom polaganja jaja, morfološke promene i populacionu dinamiku. Dodatano biće predstavljeni rezultati populacija koje su održavane u režimima za ranu i kasnu reprodukciju koje su dovele do velikih promena na molekularnom, biohemijskom i fiziološkom nivou. Konačno, posebna pažnja će biti posvećena primeni eksperimentalne evolucije u razvoju savremene metode kontrole brojnosti insekata koja se zasniva na specifičnim mitohondrijskim mutacijama s polno specifičnim efektima koji ograničavaju reproduktivni potencijal mužjaka bez negativnih efekata na ženke (tehnika trojanskih ženki).",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Aktualne i višedecenijske studije eksperimentalne evolucije na pasuljevom žišku",
pages = "93",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5034"
}

Savković, U., Đorđević, M., Budečević, S., Vlajnić, L.,& Stojković, B.. (2022). Aktualne i višedecenijske studije eksperimentalne evolucije na pasuljevom žišku. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 93.
https://hdl.handle.net/21.15107/rcub_ibiss_5034

Savković U, Đorđević M, Budečević S, Vlajnić L, Stojković B. Aktualne i višedecenijske studije eksperimentalne evolucije na pasuljevom žišku. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:93.
https://hdl.handle.net/21.15107/rcub_ibiss_5034 .

Savković, Uroš, Đorđević, Mirko, Budečević, Sanja, Vlajnić, Lea, Stojković, Biljana, "Aktualne i višedecenijske studije eksperimentalne evolucije na pasuljevom žišku" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):93,
https://hdl.handle.net/21.15107/rcub_ibiss_5034 .

TY  - CONF
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Budečević, Sanja
AU  - Vlajnić, Lea
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Stojković, Oliver
AU  - Stojković, Biljana
PY  - 2022
UR  - https://www.eseb2022.cz/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5030
AB  - Diverse aspects of insects’ behaviour, physiology, and the relationship between life-history traits are challenged when insects try to expand their host range, exploit alternative food sources and specialise on them. Process that enables phytophagous insects to utilise new food sources, known as host shift, is tightly associated with developmental plasticity and is seldom studied in laboratory settings. Using an experimental evolution approach we simulated the host shift process and observed the evolution of plasticity in seed beetle (Acanthoscelides obtectus) laboratory populations that evolved on optimal (common beans) and suboptimal (chickpea) plant hosts for more than 35 years. We have looked into: 1) life-history traits and how the long-term exposure to different hosts affects them; 2) the consequences when insects are exposed to short-term (in a single generation) change of the host plant, and 3) what happens when the host plant is altered each generation, that is, we observed  the process of the selection for increased plasticity in a laboratory setting. Prior to life-history assays, populations were in the experiment for 13 generations. We found that long-term host shift to chickpeas decreased plasticity levels for preadult traits compared to bean adapted populations. Simultaneously, fecundity evolved a more plastic response. Groups that were evolving in conditions where plant hosts were alternated each generation had the same plasticity patterns as their ancestral populations, suggesting the need for more time for plastic response to evolve. This research illustrates the importance of phenotypic plasticity in maintaining populations under changing feeding conditions.
PB  - European Society for Evolutionary Biology
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
T1  - Developmental plasticity and the potential of host shift in the seed beetle
SP  - 184
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5030
ER  - 

@conference{
author = "Savković, Uroš and Đorđević, Mirko and Budečević, Sanja and Vlajnić, Lea and Pešić, Snežana and Vukajlović, Filip and Predojević, Dragana and Mitrovski Bogdanović, Ana and Stojković, Oliver and Stojković, Biljana",
year = "2022",
abstract = "Diverse aspects of insects’ behaviour, physiology, and the relationship between life-history traits are challenged when insects try to expand their host range, exploit alternative food sources and specialise on them. Process that enables phytophagous insects to utilise new food sources, known as host shift, is tightly associated with developmental plasticity and is seldom studied in laboratory settings. Using an experimental evolution approach we simulated the host shift process and observed the evolution of plasticity in seed beetle (Acanthoscelides obtectus) laboratory populations that evolved on optimal (common beans) and suboptimal (chickpea) plant hosts for more than 35 years. We have looked into: 1) life-history traits and how the long-term exposure to different hosts affects them; 2) the consequences when insects are exposed to short-term (in a single generation) change of the host plant, and 3) what happens when the host plant is altered each generation, that is, we observed  the process of the selection for increased plasticity in a laboratory setting. Prior to life-history assays, populations were in the experiment for 13 generations. We found that long-term host shift to chickpeas decreased plasticity levels for preadult traits compared to bean adapted populations. Simultaneously, fecundity evolved a more plastic response. Groups that were evolving in conditions where plant hosts were alternated each generation had the same plasticity patterns as their ancestral populations, suggesting the need for more time for plastic response to evolve. This research illustrates the importance of phenotypic plasticity in maintaining populations under changing feeding conditions.",
publisher = "European Society for Evolutionary Biology",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic",
title = "Developmental plasticity and the potential of host shift in the seed beetle",
pages = "184",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5030"
}

Savković, U., Đorđević, M., Budečević, S., Vlajnić, L., Pešić, S., Vukajlović, F., Predojević, D., Mitrovski Bogdanović, A., Stojković, O.,& Stojković, B.. (2022). Developmental plasticity and the potential of host shift in the seed beetle. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
European Society for Evolutionary Biology., 184.
https://hdl.handle.net/21.15107/rcub_ibiss_5030

Savković U, Đorđević M, Budečević S, Vlajnić L, Pešić S, Vukajlović F, Predojević D, Mitrovski Bogdanović A, Stojković O, Stojković B. Developmental plasticity and the potential of host shift in the seed beetle. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic. 2022;:184.
https://hdl.handle.net/21.15107/rcub_ibiss_5030 .

Savković, Uroš, Đorđević, Mirko, Budečević, Sanja, Vlajnić, Lea, Pešić, Snežana, Vukajlović, Filip, Predojević, Dragana, Mitrovski Bogdanović, Ana, Stojković, Oliver, Stojković, Biljana, "Developmental plasticity and the potential of host shift in the seed beetle" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic (2022):184,
https://hdl.handle.net/21.15107/rcub_ibiss_5030 .

TY  - JOUR
AU  - Budečević, Sanja
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Stojković, Biljana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4201
AB  - Sexual dimorphism and specific patterns of development contribute in a great manner to the direction and degree of the sexual differences in body size and shape in many insects. Using a landmark-based geometric morpohometrics approach, we investigated sex-specific morphological size and shape variation in the seed beetle, Acanthoscelides obtectus. We also tested the functional hypothesis of the two morphological modules—thorax and abdomen in both sexes. Female-biased sexual dimorphism in size was shown, while differences in shape were reflected in the wider thorax and abdomen and shorter abdomen in females in comparison to males. The functional hypothesis of a two-module body was confirmed only in females before correction for size, and in both sexes after the allometry correction. Our results indicate that reproductive function has the central role in forming the patterns of modularity. We hypothesize that high morphological integration of the abdomen in females results from intense stabilizing selection, while the more relaxed integration in males is driven by the higher intensity of sexual selection.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Insects
T1  - Sexual Dimorphism and Morphological Modularity in Acanthoscelides obtectus (Say, 1831) (Coleoptera: Chrysomelidae): A Geometric Morphometric Approach
IS  - 4
VL  - 12
DO  - 10.3390/insects12040350
SP  - 350
ER  - 

@article{
author = "Budečević, Sanja and Savković, Uroš and Đorđević, Mirko and Vlajnić, Lea and Stojković, Biljana",
year = "2021",
abstract = "Sexual dimorphism and specific patterns of development contribute in a great manner to the direction and degree of the sexual differences in body size and shape in many insects. Using a landmark-based geometric morpohometrics approach, we investigated sex-specific morphological size and shape variation in the seed beetle, Acanthoscelides obtectus. We also tested the functional hypothesis of the two morphological modules—thorax and abdomen in both sexes. Female-biased sexual dimorphism in size was shown, while differences in shape were reflected in the wider thorax and abdomen and shorter abdomen in females in comparison to males. The functional hypothesis of a two-module body was confirmed only in females before correction for size, and in both sexes after the allometry correction. Our results indicate that reproductive function has the central role in forming the patterns of modularity. We hypothesize that high morphological integration of the abdomen in females results from intense stabilizing selection, while the more relaxed integration in males is driven by the higher intensity of sexual selection.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Insects",
title = "Sexual Dimorphism and Morphological Modularity in Acanthoscelides obtectus (Say, 1831) (Coleoptera: Chrysomelidae): A Geometric Morphometric Approach",
number = "4",
volume = "12",
doi = "10.3390/insects12040350",
pages = "350"
}

Budečević, S., Savković, U., Đorđević, M., Vlajnić, L.,& Stojković, B.. (2021). Sexual Dimorphism and Morphological Modularity in Acanthoscelides obtectus (Say, 1831) (Coleoptera: Chrysomelidae): A Geometric Morphometric Approach. in Insects
Multidisciplinary Digital Publishing Institute., 12(4), 350.
https://doi.org/10.3390/insects12040350

Budečević S, Savković U, Đorđević M, Vlajnić L, Stojković B. Sexual Dimorphism and Morphological Modularity in Acanthoscelides obtectus (Say, 1831) (Coleoptera: Chrysomelidae): A Geometric Morphometric Approach. in Insects. 2021;12(4):350.
doi:10.3390/insects12040350 .

Budečević, Sanja, Savković, Uroš, Đorđević, Mirko, Vlajnić, Lea, Stojković, Biljana, "Sexual Dimorphism and Morphological Modularity in Acanthoscelides obtectus (Say, 1831) (Coleoptera: Chrysomelidae): A Geometric Morphometric Approach" in Insects, 12, no. 4 (2021):350,
https://doi.org/10.3390/insects12040350 . .

TY  - THES
AU  - Budečević, Sanja
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3258
AB  - Analize unutar - i među - individualnihih razlika morfoloških struktura omogućuju otkrivanje obrazaca fenotipskog variranja, kao i ekološko - evolucionih mehanizama koji dovode do njihove divergencije. U ovoj disertaciji utvrđene su komponente fenotipske varijanse oblika funkcionalno različitih cvetnih organa kod entomofilne biljne vrste Iris pumila L., polimorfne u odnosu na boju cveta. Eksperimenti su vršeni na biljkama gajenim u eksperimentalnoj bašti, poreklom iz recipročnih ukrštanja 24 klonalna genotipa. Metodama geometrijske morfometrije utvrđeni su obrasci variranja oblika tri bilateralno simetrična organa: fola, standarda i grane tučka. Kod sva tri organa udeo simetrične komponente u ukupnom variranju oblika bio je najveći, dok je fluktuirajuća asimetrija (FA) dominirala u asimetričnoj komponenti. Iako se smatra da je primarni uzrok FA nestabilnost razvića, naša istraživanja su pokazala da fenotipska plastičnost može takođe da proizvede FA. Naime, replike cvetnih organa sa različitom orijentacijom prema suncu imale su različite vrednosti asimetričnih komponenti varijanse oblika, kao rezultat plastičnosti u odnosu na mikrosredinsku heterogenost ambijentalne svetlosti. Da veličina, oblik i boja cveta predstavljaju vizuelne signale za privlačenje potencijalnih oprašivača potvrdila su poređenja veličine cvetnih organa oprašenih i neoprašenih biljaka. Oprašeni cvetovi su, po pravilu, imali veće organe od neoprašenih, sa izuzetkom grane tučka. Regresione analize su pokazale da je veličina fola bila pod delovanjem pozitivne, a grane tučka negativne direkcione selekcije. Suprotno tome, oblik cvetnih organa se razlikovao u zavisnosti od boje, što ukazuje da je preferencija oprašivača prema specifičnim kombinacijama vizuelnih signala bila važan mehanizam morfološke divergencije reproduktivnih organa I. pumila.
AB  - Analyzes of intra - and inter - individual differences of morphological structures allow the detection of patterns of phenotypic variation, as well as ecological evolutionary mechanisms leading to their divergence. In this dissertation, components of the phenotypic variation of the shape of functionally distinctive flower organs were determined in the entomophilous plant, specific for striking flower color polymorphism, Iris pumila L. Experiments were carried out on plants grown in experimental garden, originating from reciprocal crossing of 24 clonal genotypes. Using the methods of geometric morphometrics patterns of variation of the shape of three bilaterally symmetrical organs (falls, standards and style branches) have been revealed. In all three floral organs, the proportion of the symmetric component in the overall shape variation was the highest, while the fluctuating asymmetry (FA) was dominated in asymmetric component. Although it is thought that primary cause of FA is developmental instability, our research has shown that phenotypic plasticity can also produce FA. Namely, replicas of flower organs with different orientations towards the sun had different values of asymmetric components of variance of the shape, as an outcome of plasticity in relation to the microenvironmental heterogeneity of ambient light. In this dissertation it is confirmed that size, shape and color of flowers are visual attractants for pollinators. Pairwase comparisons in size between pollinated and unpollinated flowers revealed that pollinated falls and standards are bigger related to their unpollinated pairs. Regression analyzes have shown that the size of the falls was under positive selection, while size of style branches was under negative direct selection. In contrast, the shape of the flower organs differed depending on their color, indicating that the pollinator's preference for specific combinations of visual signals was an important mechanism of morphological divergence of the reproductive organs of I. pumila.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Morfološko variranje, fenotipska plastičnost i fluktuirajuća asimetrija oblika cvetnih organa kod Iris pumila L.
T1  - Morphological variation, phenotypic plasticity and fluctuating asymmetry of floral organ shapes in Iris pumila L.
SP  - 1
EP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3258
ER  - 

@phdthesis{
author = "Budečević, Sanja",
year = "2019",
abstract = "Analize unutar - i među - individualnihih razlika morfoloških struktura omogućuju otkrivanje obrazaca fenotipskog variranja, kao i ekološko - evolucionih mehanizama koji dovode do njihove divergencije. U ovoj disertaciji utvrđene su komponente fenotipske varijanse oblika funkcionalno različitih cvetnih organa kod entomofilne biljne vrste Iris pumila L., polimorfne u odnosu na boju cveta. Eksperimenti su vršeni na biljkama gajenim u eksperimentalnoj bašti, poreklom iz recipročnih ukrštanja 24 klonalna genotipa. Metodama geometrijske morfometrije utvrđeni su obrasci variranja oblika tri bilateralno simetrična organa: fola, standarda i grane tučka. Kod sva tri organa udeo simetrične komponente u ukupnom variranju oblika bio je najveći, dok je fluktuirajuća asimetrija (FA) dominirala u asimetričnoj komponenti. Iako se smatra da je primarni uzrok FA nestabilnost razvića, naša istraživanja su pokazala da fenotipska plastičnost može takođe da proizvede FA. Naime, replike cvetnih organa sa različitom orijentacijom prema suncu imale su različite vrednosti asimetričnih komponenti varijanse oblika, kao rezultat plastičnosti u odnosu na mikrosredinsku heterogenost ambijentalne svetlosti. Da veličina, oblik i boja cveta predstavljaju vizuelne signale za privlačenje potencijalnih oprašivača potvrdila su poređenja veličine cvetnih organa oprašenih i neoprašenih biljaka. Oprašeni cvetovi su, po pravilu, imali veće organe od neoprašenih, sa izuzetkom grane tučka. Regresione analize su pokazale da je veličina fola bila pod delovanjem pozitivne, a grane tučka negativne direkcione selekcije. Suprotno tome, oblik cvetnih organa se razlikovao u zavisnosti od boje, što ukazuje da je preferencija oprašivača prema specifičnim kombinacijama vizuelnih signala bila važan mehanizam morfološke divergencije reproduktivnih organa I. pumila., Analyzes of intra - and inter - individual differences of morphological structures allow the detection of patterns of phenotypic variation, as well as ecological evolutionary mechanisms leading to their divergence. In this dissertation, components of the phenotypic variation of the shape of functionally distinctive flower organs were determined in the entomophilous plant, specific for striking flower color polymorphism, Iris pumila L. Experiments were carried out on plants grown in experimental garden, originating from reciprocal crossing of 24 clonal genotypes. Using the methods of geometric morphometrics patterns of variation of the shape of three bilaterally symmetrical organs (falls, standards and style branches) have been revealed. In all three floral organs, the proportion of the symmetric component in the overall shape variation was the highest, while the fluctuating asymmetry (FA) was dominated in asymmetric component. Although it is thought that primary cause of FA is developmental instability, our research has shown that phenotypic plasticity can also produce FA. Namely, replicas of flower organs with different orientations towards the sun had different values of asymmetric components of variance of the shape, as an outcome of plasticity in relation to the microenvironmental heterogeneity of ambient light. In this dissertation it is confirmed that size, shape and color of flowers are visual attractants for pollinators. Pairwase comparisons in size between pollinated and unpollinated flowers revealed that pollinated falls and standards are bigger related to their unpollinated pairs. Regression analyzes have shown that the size of the falls was under positive selection, while size of style branches was under negative direct selection. In contrast, the shape of the flower organs differed depending on their color, indicating that the pollinator's preference for specific combinations of visual signals was an important mechanism of morphological divergence of the reproductive organs of I. pumila.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Morfološko variranje, fenotipska plastičnost i fluktuirajuća asimetrija oblika cvetnih organa kod Iris pumila L., Morphological variation, phenotypic plasticity and fluctuating asymmetry of floral organ shapes in Iris pumila L.",
pages = "1-150",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3258"
}

Budečević, S.. (2019). Morfološko variranje, fenotipska plastičnost i fluktuirajuća asimetrija oblika cvetnih organa kod Iris pumila L.. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-150.
https://hdl.handle.net/21.15107/rcub_ibiss_3258

Budečević S. Morfološko variranje, fenotipska plastičnost i fluktuirajuća asimetrija oblika cvetnih organa kod Iris pumila L.. in University of Belgrade, Faculty of Biology. 2019;:1-150.
https://hdl.handle.net/21.15107/rcub_ibiss_3258 .

Budečević, Sanja, "Morfološko variranje, fenotipska plastičnost i fluktuirajuća asimetrija oblika cvetnih organa kod Iris pumila L." in University of Belgrade, Faculty of Biology (2019):1-150,
https://hdl.handle.net/21.15107/rcub_ibiss_3258 .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
PY  - 2019
UR  - https://app.oxfordabstracts.com/events/653/program-app/submission/123447
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3546
AB  - To predict short-term plant responses to global warming, an open-topped-chamber experiment was conducted in two sun-exposed natural populations of Iris pumila during a growing season. In each population, one half of all randomly selected clonal plants were experimentally warmed using a clear-sided, open-topped chamber (OTC), while the other half experienced the ambient temperature conditions. The air temperature within the OTCs was approx.1-2 ºC higher than that outside of the OTCs in general. In spring and summer of 2018, phenotypic values of the three functional leaf traits: specific leaf area (SLA), leaf dry mass content (LDMC), and leaf water content (LWC) were recorded on the ramets from each clone growing inside and outside of the OTC. The phenotypic expressions of all analyzed traits changed significantly with temperature in both populations, but in an opposite direction. While SLA and LWC tended to be greater in the ramets developing within the OTCs relative to those growing outside of them, their LDMC values reversed, being higher inside than outside of the OTCs. The analyzed traits varied seasonally as well. Regardless of the location within a clone, the values of SLA and LWC were greater in spring then in summer. Conversely, LDMC reached its maximum in the warmer season. Our results clearly suggest that I. pumila plants have the capacity to cope with increasing temperatures by plastic responses of leaf functional traits. This finding is of crucial importance to forecast the fate of Iris populations under global warming - that is, whether they will adopt or disappear.
PB  - The European Society for Evolutionary Biology
C3  - 17th Congress of the European Society for Evolutionary Biology
T1  - Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment
SP  - S36e.P12
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3546
ER  - 

@conference{
author = "Hočevar, Katarina and Budečević, Sanja and Vuleta, Ana and Manitašević Jovanović, Sanja",
year = "2019",
abstract = "To predict short-term plant responses to global warming, an open-topped-chamber experiment was conducted in two sun-exposed natural populations of Iris pumila during a growing season. In each population, one half of all randomly selected clonal plants were experimentally warmed using a clear-sided, open-topped chamber (OTC), while the other half experienced the ambient temperature conditions. The air temperature within the OTCs was approx.1-2 ºC higher than that outside of the OTCs in general. In spring and summer of 2018, phenotypic values of the three functional leaf traits: specific leaf area (SLA), leaf dry mass content (LDMC), and leaf water content (LWC) were recorded on the ramets from each clone growing inside and outside of the OTC. The phenotypic expressions of all analyzed traits changed significantly with temperature in both populations, but in an opposite direction. While SLA and LWC tended to be greater in the ramets developing within the OTCs relative to those growing outside of them, their LDMC values reversed, being higher inside than outside of the OTCs. The analyzed traits varied seasonally as well. Regardless of the location within a clone, the values of SLA and LWC were greater in spring then in summer. Conversely, LDMC reached its maximum in the warmer season. Our results clearly suggest that I. pumila plants have the capacity to cope with increasing temperatures by plastic responses of leaf functional traits. This finding is of crucial importance to forecast the fate of Iris populations under global warming - that is, whether they will adopt or disappear.",
publisher = "The European Society for Evolutionary Biology",
journal = "17th Congress of the European Society for Evolutionary Biology",
title = "Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment",
pages = "S36e.P12",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3546"
}

Hočevar, K., Budečević, S., Vuleta, A.,& Manitašević Jovanović, S.. (2019). Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment. in 17th Congress of the European Society for Evolutionary Biology
The European Society for Evolutionary Biology., S36e.P12.
https://hdl.handle.net/21.15107/rcub_ibiss_3546

Hočevar K, Budečević S, Vuleta A, Manitašević Jovanović S. Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment. in 17th Congress of the European Society for Evolutionary Biology. 2019;:S36e.P12.
https://hdl.handle.net/21.15107/rcub_ibiss_3546 .

Hočevar, Katarina, Budečević, Sanja, Vuleta, Ana, Manitašević Jovanović, Sanja, "Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment" in 17th Congress of the European Society for Evolutionary Biology (2019):S36e.P12,
https://hdl.handle.net/21.15107/rcub_ibiss_3546 .

TY  - CONF
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
AU  - Hočevar, Katarina
AU  - Tucić, Branka
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5061
AB  - Flowers are complex phenotypic structures composed of four functionally distinct organs arranged in concentric floral whorls: sepals in the frst, petals in the second, and two inner whorls
consisting of reproductive structures: stamens in the third whorl and tricarpellary gynoecium, in
the centre. Color, size and shape of these floral organs are considered to be important signals that
can attract the pollinators, advertising the plants’ floral reward (commonly nectar and pollen).
Here we use the methods of geometric morphometrics to compare size and shape variation of
three functionally different floral organs: falls, standards and style arms, among fve distinct color
morphs (blue, violet, dark blue, dark violet and yellow). Pairwise comparisons of the centroid size
means showed that all floral organs differed signifcantly among the analysed color phenotypes.
The mean shape of falls, standards and style arms appeared to be flower color-specifc as well, but
most noticeably between yellow - on one side, and blue/violet morphs - on the other. Procrustes
ANOVAs revealed the signifcant presence of directional and fluctuating asymmetry for falls and
standards among all fve color morphs, with the exception of style arms. The observed morphological diversifcation of floral organs among distinctive I. pumila color phenotypes could be the
adaptive outcome of the interactions with their pollinators.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5061
ER  - 

@conference{
author = "Budečević, Sanja and Manitašević Jovanović, Sanja and Vuleta, Ana and Hočevar, Katarina and Tucić, Branka",
year = "2018",
abstract = "Flowers are complex phenotypic structures composed of four functionally distinct organs arranged in concentric floral whorls: sepals in the frst, petals in the second, and two inner whorls
consisting of reproductive structures: stamens in the third whorl and tricarpellary gynoecium, in
the centre. Color, size and shape of these floral organs are considered to be important signals that
can attract the pollinators, advertising the plants’ floral reward (commonly nectar and pollen).
Here we use the methods of geometric morphometrics to compare size and shape variation of
three functionally different floral organs: falls, standards and style arms, among fve distinct color
morphs (blue, violet, dark blue, dark violet and yellow). Pairwise comparisons of the centroid size
means showed that all floral organs differed signifcantly among the analysed color phenotypes.
The mean shape of falls, standards and style arms appeared to be flower color-specifc as well, but
most noticeably between yellow - on one side, and blue/violet morphs - on the other. Procrustes
ANOVAs revealed the signifcant presence of directional and fluctuating asymmetry for falls and
standards among all fve color morphs, with the exception of style arms. The observed morphological diversifcation of floral organs among distinctive I. pumila color phenotypes could be the
adaptive outcome of the interactions with their pollinators.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?",
pages = "82",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5061"
}

Budečević, S., Manitašević Jovanović, S., Vuleta, A., Hočevar, K.,& Tucić, B.. (2018). Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 82.
https://hdl.handle.net/21.15107/rcub_ibiss_5061

Budečević S, Manitašević Jovanović S, Vuleta A, Hočevar K, Tucić B. Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:82.
https://hdl.handle.net/21.15107/rcub_ibiss_5061 .

Budečević, Sanja, Manitašević Jovanović, Sanja, Vuleta, Ana, Hočevar, Katarina, Tucić, Branka, "Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):82,
https://hdl.handle.net/21.15107/rcub_ibiss_5061 .

TY  - CONF
AU  - Vuleta, Ana
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5062
AB  - Angiosperm flowers serve as an advertisement for pollinators and are, therefore, believed to
be shaped by pollinator-mediated selection. The large flower size, which is one of the distinct
characteristics of the genus Iris, might have evolved under the strong selective pressures imposed
by pollinators, either because larger flowers indicate more rewards or because the pollinators can
detect them from a greater distance. To test the role of visual floral signals in attracting pollinators and, consequently, pollination efciency, we compared the phenotypic expressions of flower
height and centroid size of petaloid floral organs: falls, standards and style arms, as well as anthocyanin absorption between naturally pollinated and non-pollinated flowers of I. pumila plants
grown in a common-garden experiment. Our results indicated that I. pumila pollinators generally
preferred taller flowers with greater organ sizes compared to the alternative ones. However, the
direction of pollinator-mediated selection appeared to be strongly flower organ-specifc: positive
on fall, negative on style arm and neutral on standard size. The observed results are in agreement
with the functions that each of these floral structures has in the pollination process: standards are
a long-distance reward signals, falls are landing platform for pollinating insects, while style arms,
as upper parts of the pollination tunnels, promote pollen deposition. We failed to corroborate the
existence of pollinator-mediated selection on anthocyanin absorption. This suggested that some
other aspects of flower colour might be more attractive to insect pollinators, or that different biotic or abiotic factors could account for the maintenance of flower colour polymorphism in I. pumila.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Do pollinators prefer bigger floral organs? A case study on Iris pumila L.
SP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5062
ER  - 

@conference{
author = "Vuleta, Ana and Budečević, Sanja and Manitašević Jovanović, Sanja and Tucić, Branka",
year = "2018",
abstract = "Angiosperm flowers serve as an advertisement for pollinators and are, therefore, believed to
be shaped by pollinator-mediated selection. The large flower size, which is one of the distinct
characteristics of the genus Iris, might have evolved under the strong selective pressures imposed
by pollinators, either because larger flowers indicate more rewards or because the pollinators can
detect them from a greater distance. To test the role of visual floral signals in attracting pollinators and, consequently, pollination efciency, we compared the phenotypic expressions of flower
height and centroid size of petaloid floral organs: falls, standards and style arms, as well as anthocyanin absorption between naturally pollinated and non-pollinated flowers of I. pumila plants
grown in a common-garden experiment. Our results indicated that I. pumila pollinators generally
preferred taller flowers with greater organ sizes compared to the alternative ones. However, the
direction of pollinator-mediated selection appeared to be strongly flower organ-specifc: positive
on fall, negative on style arm and neutral on standard size. The observed results are in agreement
with the functions that each of these floral structures has in the pollination process: standards are
a long-distance reward signals, falls are landing platform for pollinating insects, while style arms,
as upper parts of the pollination tunnels, promote pollen deposition. We failed to corroborate the
existence of pollinator-mediated selection on anthocyanin absorption. This suggested that some
other aspects of flower colour might be more attractive to insect pollinators, or that different biotic or abiotic factors could account for the maintenance of flower colour polymorphism in I. pumila.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Do pollinators prefer bigger floral organs? A case study on Iris pumila L.",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5062"
}

Vuleta, A., Budečević, S., Manitašević Jovanović, S.,& Tucić, B.. (2018). Do pollinators prefer bigger floral organs? A case study on Iris pumila L.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 86.
https://hdl.handle.net/21.15107/rcub_ibiss_5062

Vuleta A, Budečević S, Manitašević Jovanović S, Tucić B. Do pollinators prefer bigger floral organs? A case study on Iris pumila L.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:86.
https://hdl.handle.net/21.15107/rcub_ibiss_5062 .

Vuleta, Ana, Budečević, Sanja, Manitašević Jovanović, Sanja, Tucić, Branka, "Do pollinators prefer bigger floral organs? A case study on Iris pumila L." in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):86,
https://hdl.handle.net/21.15107/rcub_ibiss_5062 .

TY  - JOUR
AU  - Tucić, Branka
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
AU  - Klingenberg, Christian Peter
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jeb.13207
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29134739
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2927
AB  - Fluctuating asymmetry (FA) is widely used to quantify developmental instability (DI) in ecological and evolutionary studies. It has long been recognized that FA may not exclusively originate from DI for sessile organisms such as plants, because phenotypic plasticity in response to heterogeneities in the environment might also produce FA. This study provides the first empirical evidence for this hypothesis. We reasoned that solar irradiance, which is greater on the southern side than on the northern side of plants growing in the temperate zone of the Northern Hemisphere, would cause systematic morphological differences and asymmetry associated with the orientation of plant parts. We used geometric morphometrics to characterize the size and shape of flower parts in Iris pumila grown in a common garden. The size of floral organs was not significantly affected by orientation. Shape and particularly its asymmetric component differed significantly according to orientation for three different floral parts. Orientation accounted for 10.4% of the total shape asymmetry within flowers in the falls, for 11.4% in the standards and for 2.2% in the style branches. This indicates that phenotypic plasticity in response to a directed environmental factor, most likely solar irradiance, contributes to FA of flowers under natural conditions. That FA partly results from phenotypic plasticity and not just from DI needs to be considered by studies of FA in plants and other sessile organisms.
T2  - Journal of Evolutionary Biology
T1  - Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.
IS  - 2
VL  - 31
DO  - 10.1111/jeb.13207
SP  - 197
EP  - 210
ER  - 

@article{
author = "Tucić, Branka and Budečević, Sanja and Manitašević Jovanović, Sanja and Vuleta, Ana and Klingenberg, Christian Peter",
year = "2018",
abstract = "Fluctuating asymmetry (FA) is widely used to quantify developmental instability (DI) in ecological and evolutionary studies. It has long been recognized that FA may not exclusively originate from DI for sessile organisms such as plants, because phenotypic plasticity in response to heterogeneities in the environment might also produce FA. This study provides the first empirical evidence for this hypothesis. We reasoned that solar irradiance, which is greater on the southern side than on the northern side of plants growing in the temperate zone of the Northern Hemisphere, would cause systematic morphological differences and asymmetry associated with the orientation of plant parts. We used geometric morphometrics to characterize the size and shape of flower parts in Iris pumila grown in a common garden. The size of floral organs was not significantly affected by orientation. Shape and particularly its asymmetric component differed significantly according to orientation for three different floral parts. Orientation accounted for 10.4% of the total shape asymmetry within flowers in the falls, for 11.4% in the standards and for 2.2% in the style branches. This indicates that phenotypic plasticity in response to a directed environmental factor, most likely solar irradiance, contributes to FA of flowers under natural conditions. That FA partly results from phenotypic plasticity and not just from DI needs to be considered by studies of FA in plants and other sessile organisms.",
journal = "Journal of Evolutionary Biology",
title = "Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.",
number = "2",
volume = "31",
doi = "10.1111/jeb.13207",
pages = "197-210"
}

Tucić, B., Budečević, S., Manitašević Jovanović, S., Vuleta, A.,& Klingenberg, C. P.. (2018). Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.. in Journal of Evolutionary Biology, 31(2), 197-210.
https://doi.org/10.1111/jeb.13207

Tucić B, Budečević S, Manitašević Jovanović S, Vuleta A, Klingenberg CP. Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.. in Journal of Evolutionary Biology. 2018;31(2):197-210.
doi:10.1111/jeb.13207 .

Tucić, Branka, Budečević, Sanja, Manitašević Jovanović, Sanja, Vuleta, Ana, Klingenberg, Christian Peter, "Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence." in Journal of Evolutionary Biology, 31, no. 2 (2018):197-210,
https://doi.org/10.1111/jeb.13207 . .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5059
AB  - The 70kD heat shock proteins (Hsp70s) are highly conserved molecular chaperones that play
essential roles in numerous cellular processes, including the folding of newly synthesized and
stress-denatured proteins, the translocation of proteins across membranes, as well as the degradation of aggregated proteins. Here we examined the variation in the abundance of Hsp70s in
different floral organs of the three distinctive color morphs in Iris pumila. Flowers of twenty seven
genotypes raised in a common-garden and belonging to three colour classes: yellow, dark blue
and dark violet, were collected (9 per each class) for an immunoblot analysis. The content of Hsp70
was quantifed in four floral organs: falls, standards, stamens and style arms. The immunoblot analysis revealed that the expression of Hsp70s was floral organ-specifc. While only one Hsp70 isoform
was expressed in the standards and falls, the stamens and style arms expressed two isoforms. The
relative abundance of Hsp70 varied among differed flower organs as well. The lowest value was
recorded in the standards and the highest in the stamens, whereas its abundance in the falls and
style arms appeared to be intermediate. Such variation pattern was detected exclusively in the
dark violet colour morphs. In the yellow flowers, however, the amount of Hsp70 tended to be lower compared to the dark blue or dark violet colour morphs. This study provides original evidence
for organ-specifc Hsp70 expression in flowers of I. pumila plants, likely due to the differential metabolic activities occurring in the cells of these functionally distinctive floral structures.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila
SP  - 89
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5059
ER  - 

@conference{
author = "Hočevar, Katarina and Budečević, Sanja and Manitašević Jovanović, Sanja",
year = "2018",
abstract = "The 70kD heat shock proteins (Hsp70s) are highly conserved molecular chaperones that play
essential roles in numerous cellular processes, including the folding of newly synthesized and
stress-denatured proteins, the translocation of proteins across membranes, as well as the degradation of aggregated proteins. Here we examined the variation in the abundance of Hsp70s in
different floral organs of the three distinctive color morphs in Iris pumila. Flowers of twenty seven
genotypes raised in a common-garden and belonging to three colour classes: yellow, dark blue
and dark violet, were collected (9 per each class) for an immunoblot analysis. The content of Hsp70
was quantifed in four floral organs: falls, standards, stamens and style arms. The immunoblot analysis revealed that the expression of Hsp70s was floral organ-specifc. While only one Hsp70 isoform
was expressed in the standards and falls, the stamens and style arms expressed two isoforms. The
relative abundance of Hsp70 varied among differed flower organs as well. The lowest value was
recorded in the standards and the highest in the stamens, whereas its abundance in the falls and
style arms appeared to be intermediate. Such variation pattern was detected exclusively in the
dark violet colour morphs. In the yellow flowers, however, the amount of Hsp70 tended to be lower compared to the dark blue or dark violet colour morphs. This study provides original evidence
for organ-specifc Hsp70 expression in flowers of I. pumila plants, likely due to the differential metabolic activities occurring in the cells of these functionally distinctive floral structures.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila",
pages = "89-90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5059"
}

Hočevar, K., Budečević, S.,& Manitašević Jovanović, S.. (2018). Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 89-90.
https://hdl.handle.net/21.15107/rcub_ibiss_5059

Hočevar K, Budečević S, Manitašević Jovanović S. Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:89-90.
https://hdl.handle.net/21.15107/rcub_ibiss_5059 .

Hočevar, Katarina, Budečević, Sanja, Manitašević Jovanović, Sanja, "Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):89-90,
https://hdl.handle.net/21.15107/rcub_ibiss_5059 .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5060
AB  - The 70kD heat shock proteins (Hsp70s) are highly conserved molecular chaperones that playessential roles in numerous cellular processes, including the folding of newly synthesized andstress-denatured proteins, the translocation of proteins across membranes, as well as the degradation of aggregated proteins. Here we examined the variation in the abundance of Hsp70s indifferent floral organs of the three distinctive color morphs in Iris pumila. Flowers of twenty sevengenotypes raised in a common-garden and belonging to three colour classes: yellow, dark blueand dark violet, were collected (9 per each class) for an immunoblot analysis. The content of Hsp70was quantifed in four floral organs: falls, standards, stamens and style arms. The immunoblot analysis revealed that the expression of Hsp70s was floral organ-specifc. While only one Hsp70 isoformwas expressed in the standards and falls, the stamens and style arms expressed two isoforms. Therelative abundance of Hsp70 varied among differed flower organs as well. The lowest value wasrecorded in the standards and the highest in the stamens, whereas its abundance in the falls andstyle arms appeared to be intermediate. Such variation pattern was detected exclusively in thedark violet colour morphs. In the yellow flowers, however, the amount of Hsp70 tended to be lower compared to the dark blue or dark violet colour morphs. This study provides original evidencefor organ-specifc Hsp70 expression in flowers of I. pumila plants, likely due to the differential metabolic activities occurring in the cells of these functionally distinctive floral structures.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila
SP  - PP3-8
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5060
ER  - 

@conference{
author = "Hočevar, Katarina and Budečević, Sanja and Manitašević Jovanović, Sanja",
year = "2018",
abstract = "The 70kD heat shock proteins (Hsp70s) are highly conserved molecular chaperones that playessential roles in numerous cellular processes, including the folding of newly synthesized andstress-denatured proteins, the translocation of proteins across membranes, as well as the degradation of aggregated proteins. Here we examined the variation in the abundance of Hsp70s indifferent floral organs of the three distinctive color morphs in Iris pumila. Flowers of twenty sevengenotypes raised in a common-garden and belonging to three colour classes: yellow, dark blueand dark violet, were collected (9 per each class) for an immunoblot analysis. The content of Hsp70was quantifed in four floral organs: falls, standards, stamens and style arms. The immunoblot analysis revealed that the expression of Hsp70s was floral organ-specifc. While only one Hsp70 isoformwas expressed in the standards and falls, the stamens and style arms expressed two isoforms. Therelative abundance of Hsp70 varied among differed flower organs as well. The lowest value wasrecorded in the standards and the highest in the stamens, whereas its abundance in the falls andstyle arms appeared to be intermediate. Such variation pattern was detected exclusively in thedark violet colour morphs. In the yellow flowers, however, the amount of Hsp70 tended to be lower compared to the dark blue or dark violet colour morphs. This study provides original evidencefor organ-specifc Hsp70 expression in flowers of I. pumila plants, likely due to the differential metabolic activities occurring in the cells of these functionally distinctive floral structures.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila",
pages = "PP3-8",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5060"
}

Hočevar, K., Budečević, S.,& Manitašević Jovanović, S.. (2018). Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade., PP3-8.
https://hdl.handle.net/21.15107/rcub_ibiss_5060

Hočevar K, Budečević S, Manitašević Jovanović S. Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:PP3-8.
https://hdl.handle.net/21.15107/rcub_ibiss_5060 .

Hočevar, Katarina, Budečević, Sanja, Manitašević Jovanović, Sanja, "Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):PP3-8,
https://hdl.handle.net/21.15107/rcub_ibiss_5060 .

TY  - CONF
AU  - Manitašević Jovanović, Sanja
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5057
AB  - Flavonoids and carotenoids are plant pigments that play a variety of functional roles in higher
plants. Beside their impacts on flower color, they are involved in plant growth, development and
protection against a wide range of environmental stressors. They act either as light attenuators
and free radical scavengers (e.g. anthocyanins) or participate in non-photochemical quenching
(carotenoids). To quantify the accumulation of flavonoids and carotenoids in different floral organs,
ffty Iris pumila genotypes grown in a common-garden and belonging to different color classes
(yellow, blue, violet, dark blue and dark violet) were assessed. One flower per each genotype was
harvested and analyzed spectrophotometrically for the content of three flavonoids (anthocyanins,
flavones, flavonols) and total carotenoids in different floral organs: falls, standards, stamens and
style arms. While the quantity of flavonoids was found to be floral organ- and color morph-specific, the amount of carotenoids varied only among individual floral organs. The variation pattern of
the analyzed compounds displayed identical trend - the lowest amounts were recorded in the stamen and style arm, and the highest in the standard and fall. The content of carotenoids appeared
to be the highest in floral organs of the yellow color morphs, whereas in the blue, violet, dark blue
and dark violet flower phenotypes its abundance was a half of that recorded for the yellow one.
Conversely, the anthocyanin content was rather high in the floral organs of dark violet and dark
blue color morphs, but extremely low in those of the yellow one. Our results clearly suggest that a
greater accumulation of both plant pigments in sterile rather than in fertile flower organs could be
causally related to their specifc function in the process of sexual reproduction, where the former
serve to attract plant pollinators, while the latter promote an efcient pollen deposition.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Flavonoid and carotenoid content in floral organs of Iris pumila
SP  - 93
EP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5057
ER  - 

@conference{
author = "Manitašević Jovanović, Sanja and Hočevar, Katarina and Budečević, Sanja",
year = "2018",
abstract = "Flavonoids and carotenoids are plant pigments that play a variety of functional roles in higher
plants. Beside their impacts on flower color, they are involved in plant growth, development and
protection against a wide range of environmental stressors. They act either as light attenuators
and free radical scavengers (e.g. anthocyanins) or participate in non-photochemical quenching
(carotenoids). To quantify the accumulation of flavonoids and carotenoids in different floral organs,
ffty Iris pumila genotypes grown in a common-garden and belonging to different color classes
(yellow, blue, violet, dark blue and dark violet) were assessed. One flower per each genotype was
harvested and analyzed spectrophotometrically for the content of three flavonoids (anthocyanins,
flavones, flavonols) and total carotenoids in different floral organs: falls, standards, stamens and
style arms. While the quantity of flavonoids was found to be floral organ- and color morph-specific, the amount of carotenoids varied only among individual floral organs. The variation pattern of
the analyzed compounds displayed identical trend - the lowest amounts were recorded in the stamen and style arm, and the highest in the standard and fall. The content of carotenoids appeared
to be the highest in floral organs of the yellow color morphs, whereas in the blue, violet, dark blue
and dark violet flower phenotypes its abundance was a half of that recorded for the yellow one.
Conversely, the anthocyanin content was rather high in the floral organs of dark violet and dark
blue color morphs, but extremely low in those of the yellow one. Our results clearly suggest that a
greater accumulation of both plant pigments in sterile rather than in fertile flower organs could be
causally related to their specifc function in the process of sexual reproduction, where the former
serve to attract plant pollinators, while the latter promote an efcient pollen deposition.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Flavonoid and carotenoid content in floral organs of Iris pumila",
pages = "93-94",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5057"
}

Manitašević Jovanović, S., Hočevar, K.,& Budečević, S.. (2018). Flavonoid and carotenoid content in floral organs of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 93-94.
https://hdl.handle.net/21.15107/rcub_ibiss_5057

Manitašević Jovanović S, Hočevar K, Budečević S. Flavonoid and carotenoid content in floral organs of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:93-94.
https://hdl.handle.net/21.15107/rcub_ibiss_5057 .

Manitašević Jovanović, Sanja, Hočevar, Katarina, Budečević, Sanja, "Flavonoid and carotenoid content in floral organs of Iris pumila" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):93-94,
https://hdl.handle.net/21.15107/rcub_ibiss_5057 .