TY  - JOUR
AU  - Ferree, Patrick M.
AU  - Blagojević, Jelena
AU  - Houben, Andreas
AU  - Martins, Cesar
AU  - Trifonov, Vladimir A.
AU  - Vujošević, Mladen
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6686
AB  - Since the discovery of B chromosomes, multiple different definitions of these selfish genetic elements have been put forth. We reconsidered early definitions in light of recently published studies. While there are many characteristics that vary among different B chromosomes, such as their evolutionary origins, size, segregation behaviors, gene content, and function, there is one defining trait of all B chromosomes: they are nonessential for the organism. The points raised here may be useful for framing future B chromosome studies and help guide the categorization of new chromosomal elements that are uncovered in genomic studies.
PB  - Oxford University Press
T2  - G3: Genes|Genomes|Genetics
T1  - What is a B chromosome? Early definitions revisited
DO  - 10.1093/g3journal/jkae068
SP  - jkae068
ER  - 

@article{
author = "Ferree, Patrick M. and Blagojević, Jelena and Houben, Andreas and Martins, Cesar and Trifonov, Vladimir A. and Vujošević, Mladen",
year = "2024",
abstract = "Since the discovery of B chromosomes, multiple different definitions of these selfish genetic elements have been put forth. We reconsidered early definitions in light of recently published studies. While there are many characteristics that vary among different B chromosomes, such as their evolutionary origins, size, segregation behaviors, gene content, and function, there is one defining trait of all B chromosomes: they are nonessential for the organism. The points raised here may be useful for framing future B chromosome studies and help guide the categorization of new chromosomal elements that are uncovered in genomic studies.",
publisher = "Oxford University Press",
journal = "G3: Genes|Genomes|Genetics",
title = "What is a B chromosome? Early definitions revisited",
doi = "10.1093/g3journal/jkae068",
pages = "jkae068"
}

Ferree, P. M., Blagojević, J., Houben, A., Martins, C., Trifonov, V. A.,& Vujošević, M.. (2024). What is a B chromosome? Early definitions revisited. in G3: Genes|Genomes|Genetics
Oxford University Press., jkae068.
https://doi.org/10.1093/g3journal/jkae068

Ferree PM, Blagojević J, Houben A, Martins C, Trifonov VA, Vujošević M. What is a B chromosome? Early definitions revisited. in G3: Genes|Genomes|Genetics. 2024;:jkae068.
doi:10.1093/g3journal/jkae068 .

Ferree, Patrick M., Blagojević, Jelena, Houben, Andreas, Martins, Cesar, Trifonov, Vladimir A., Vujošević, Mladen, "What is a B chromosome? Early definitions revisited" in G3: Genes|Genomes|Genetics (2024):jkae068,
https://doi.org/10.1093/g3journal/jkae068 . .

TY  - JOUR
AU  - Budinski, Ivana
AU  - Bajić, Branka
AU  - Rajičić, Marija
AU  - Paunović, Milan
AU  - Miljević, Milan
AU  - Vujošević, Mladen
AU  - Blagojević, Jelena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6496
AB  - Accurate species identification is crucial for ecological research and effective wildlife management. Advances in molecular genetic tools enable this, even for cryptic species complexes that are often morphologically confusing or indistinguishable. However, the costs of these methods and sequencing remain prohibitive for many researchers, particularly in less developed regions. The aim of this study was to test whether ISSR-PCR markers can be used to distinguish three similar horseshoe bat species that are often misidentified and occur in sympatry in Serbia. Samples from 64 Rhinolophus euryale, R. blasii, and
R. mehelyi bats were identified using this ISSR-PCR approach, and species identifications were additionally confirmed by sequencing D-loop fragment of mitochondrial DNA. ISSR-PCR yielded species-specific band patterns on the agarose gel that allowed the differentiation of three medium-sized horseshoe bat species. This approach does not require sequencing, making it a quick and inexpensive tool for the genetic identification of these species, and complements already existing methods. There is potential to scale up this method to other cryptic species complexes, reducing misidentifications that lead to inaccurate population trend assessments and have knock-on effects on our ability to monitor and conserve wildlife, especially rare and endangered species.
PB  - Springer Nature
T2  - Mammal Research
T1  - Rapid and reliable method for identification of three medium‑sized horseshoe bat species in Europe
DO  - 10.1007/s13364-023-00735-w
ER  - 

@article{
author = "Budinski, Ivana and Bajić, Branka and Rajičić, Marija and Paunović, Milan and Miljević, Milan and Vujošević, Mladen and Blagojević, Jelena",
year = "2024",
abstract = "Accurate species identification is crucial for ecological research and effective wildlife management. Advances in molecular genetic tools enable this, even for cryptic species complexes that are often morphologically confusing or indistinguishable. However, the costs of these methods and sequencing remain prohibitive for many researchers, particularly in less developed regions. The aim of this study was to test whether ISSR-PCR markers can be used to distinguish three similar horseshoe bat species that are often misidentified and occur in sympatry in Serbia. Samples from 64 Rhinolophus euryale, R. blasii, and
R. mehelyi bats were identified using this ISSR-PCR approach, and species identifications were additionally confirmed by sequencing D-loop fragment of mitochondrial DNA. ISSR-PCR yielded species-specific band patterns on the agarose gel that allowed the differentiation of three medium-sized horseshoe bat species. This approach does not require sequencing, making it a quick and inexpensive tool for the genetic identification of these species, and complements already existing methods. There is potential to scale up this method to other cryptic species complexes, reducing misidentifications that lead to inaccurate population trend assessments and have knock-on effects on our ability to monitor and conserve wildlife, especially rare and endangered species.",
publisher = "Springer Nature",
journal = "Mammal Research",
title = "Rapid and reliable method for identification of three medium‑sized horseshoe bat species in Europe",
doi = "10.1007/s13364-023-00735-w"
}

Budinski, I., Bajić, B., Rajičić, M., Paunović, M., Miljević, M., Vujošević, M.,& Blagojević, J.. (2024). Rapid and reliable method for identification of three medium‑sized horseshoe bat species in Europe. in Mammal Research
Springer Nature..
https://doi.org/10.1007/s13364-023-00735-w

Budinski I, Bajić B, Rajičić M, Paunović M, Miljević M, Vujošević M, Blagojević J. Rapid and reliable method for identification of three medium‑sized horseshoe bat species in Europe. in Mammal Research. 2024;.
doi:10.1007/s13364-023-00735-w .

Budinski, Ivana, Bajić, Branka, Rajičić, Marija, Paunović, Milan, Miljević, Milan, Vujošević, Mladen, Blagojević, Jelena, "Rapid and reliable method for identification of three medium‑sized horseshoe bat species in Europe" in Mammal Research (2024),
https://doi.org/10.1007/s13364-023-00735-w . .

TY  - CONF
AU  - Vujošević, Mladen
AU  - Rajičić, Marija
AU  - Budinski, Ivana
AU  - Bajić, Branka
AU  - Adnađević, Tanja
AU  - Blagojević, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6429
AB  - B chromosomes (Bs) are uncommon in mammals, featuring less than 2% of species, but six out of 22 species possess them in the genus Apodemus. In Serbia, over 40 populations of yellow-necked mice
(Apodemus flavicollis) with varying habitat quality were studied. The frequency of B carriers (range: 0.09 to 0.67) increased with elevation, correlated with sub-zero days, and inversely with average temperature. B carrier frequency remained stable over eight years despite population density fluctuations, while seasonal variations linked to population size were observed. Overcrowding stress reduced B carriers among pre-reproductive individuals but enhanced survival in suboptimal conditions.
Phenotypic traits and B frequency were correlated, influencing cranial morphometric development. B carriers showed distinct developmental pathways for cranial traits, suggesting environment-specific benefits. Bs’ presence did not affect carrier fecundity or fertility and was evenly distributed across age groups. B chromosomes (up to five) displayed common structures across populations in Serbia and Eastern Europe, possibly originating from sex chromosome pericentromeric regions. B-specific chromatin spatially resembled pericentromeric sex chromosomes, suggesting a similar mechanism to bypass meiotic
checkpoints.
Current data support a heterotic model in A. flavicollis. Bs likely contribute to species adaptability by increasing genetic variability, potentially expanding their distribution.
PB  - Springer Nature
C3  - Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia
T1  - B chromosomes in populations of Apodemus flavicollis – never ending story
DO  - 10.1186/s12919-023-00284-9
SP  - 33
ER  - 

@conference{
author = "Vujošević, Mladen and Rajičić, Marija and Budinski, Ivana and Bajić, Branka and Adnađević, Tanja and Blagojević, Jelena",
year = "2023",
abstract = "B chromosomes (Bs) are uncommon in mammals, featuring less than 2% of species, but six out of 22 species possess them in the genus Apodemus. In Serbia, over 40 populations of yellow-necked mice
(Apodemus flavicollis) with varying habitat quality were studied. The frequency of B carriers (range: 0.09 to 0.67) increased with elevation, correlated with sub-zero days, and inversely with average temperature. B carrier frequency remained stable over eight years despite population density fluctuations, while seasonal variations linked to population size were observed. Overcrowding stress reduced B carriers among pre-reproductive individuals but enhanced survival in suboptimal conditions.
Phenotypic traits and B frequency were correlated, influencing cranial morphometric development. B carriers showed distinct developmental pathways for cranial traits, suggesting environment-specific benefits. Bs’ presence did not affect carrier fecundity or fertility and was evenly distributed across age groups. B chromosomes (up to five) displayed common structures across populations in Serbia and Eastern Europe, possibly originating from sex chromosome pericentromeric regions. B-specific chromatin spatially resembled pericentromeric sex chromosomes, suggesting a similar mechanism to bypass meiotic
checkpoints.
Current data support a heterotic model in A. flavicollis. Bs likely contribute to species adaptability by increasing genetic variability, potentially expanding their distribution.",
publisher = "Springer Nature",
journal = "Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia",
title = "B chromosomes in populations of Apodemus flavicollis – never ending story",
doi = "10.1186/s12919-023-00284-9",
pages = "33"
}

Vujošević, M., Rajičić, M., Budinski, I., Bajić, B., Adnađević, T.,& Blagojević, J.. (2023). B chromosomes in populations of Apodemus flavicollis – never ending story. in Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia
Springer Nature., 33.
https://doi.org/10.1186/s12919-023-00284-9

Vujošević M, Rajičić M, Budinski I, Bajić B, Adnađević T, Blagojević J. B chromosomes in populations of Apodemus flavicollis – never ending story. in Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia. 2023;:33.
doi:10.1186/s12919-023-00284-9 .

Vujošević, Mladen, Rajičić, Marija, Budinski, Ivana, Bajić, Branka, Adnađević, Tanja, Blagojević, Jelena, "B chromosomes in populations of Apodemus flavicollis – never ending story" in Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia (2023):33,
https://doi.org/10.1186/s12919-023-00284-9 . .

TY  - CONF
AU  - Blagojević, Jelena
AU  - Malkov, Saša
AU  - Rajičić, Marija
AU  - Rončević, Aleksa
AU  - Budinski, Ivana
AU  - Bajić, Branka
AU  - Miljević, Milan
AU  - Vujošević, Mladen
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6428
AB  - Background
Long-term studies on B chromosome (Bs) dynamics in natural populations of yellow-necked mice, Apodemus flavicollis, have unveiled intriguing seasonal variations influenced by environmental factors. On average, one third of animals possessed Bs in large natural populations. Urbanization, a potent catalyst for genetic diversity alterations, brings about habitat fragmentation and degradation, producing patches of small forested areas within urban landscapes. These fragmented habitats pose significant challenges for small mammal populations as migration and gene flow are severely restricted. To comprehensively grasp the repercussions of urbanization on B chromosome frequency, we embarked on a study within the Belgrade region.
Materials and Methods
The frequency of individuals with B chromosomes in five urban isolated forests populations of A. flavicollis has been studied. Employing live animal traps, we collected samples, and chromosome preparation was done directly from the bone marrow. Each animal’s karyotype was analysed through examination of twenty metaphase figures.
Results
A total of 246 animals were sampled across five forest sites within the Belgrade territory and underwent karyotyping. The prevalence of animals with Bs exhibited remarkable variability, ranging from 3% to 50%.
Intriguingly, at four of the sites, the incidence of Bs was significantly lower (ranging from 3% to 13%) compared to the average frequencies observed in natural populations. Conversely, at one site, the frequency soared to an exceptionally high 50%. Since all studied localities are at short distances from each other, climatic factors could be considered as the same. However, the studied sites differ in size and the level of adaptation to people’s urban life. In a bid to elucidate the driving factors behind frequency variation, we developed a model simulating the influence of genetic drift, the predominant evolutionary force in small, isolated populations.
Conclusions
Beyond investigations focused on the molecular structure and origin of B chromosomes, it becomes evident that a comprehensive understanding necessitates population-level research, shedding light on
the intricate mechanisms governing their persistence across diverse environments.
PB  - Springer Nature
C3  - Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia
T1  - Variation in the frequency of B chromosomes in small isolated populations of Apodemus flavicollis
DO  - 10.1186/s12919-023-00284-9
SP  - 33
ER  - 

@conference{
author = "Blagojević, Jelena and Malkov, Saša and Rajičić, Marija and Rončević, Aleksa and Budinski, Ivana and Bajić, Branka and Miljević, Milan and Vujošević, Mladen",
year = "2023",
abstract = "Background
Long-term studies on B chromosome (Bs) dynamics in natural populations of yellow-necked mice, Apodemus flavicollis, have unveiled intriguing seasonal variations influenced by environmental factors. On average, one third of animals possessed Bs in large natural populations. Urbanization, a potent catalyst for genetic diversity alterations, brings about habitat fragmentation and degradation, producing patches of small forested areas within urban landscapes. These fragmented habitats pose significant challenges for small mammal populations as migration and gene flow are severely restricted. To comprehensively grasp the repercussions of urbanization on B chromosome frequency, we embarked on a study within the Belgrade region.
Materials and Methods
The frequency of individuals with B chromosomes in five urban isolated forests populations of A. flavicollis has been studied. Employing live animal traps, we collected samples, and chromosome preparation was done directly from the bone marrow. Each animal’s karyotype was analysed through examination of twenty metaphase figures.
Results
A total of 246 animals were sampled across five forest sites within the Belgrade territory and underwent karyotyping. The prevalence of animals with Bs exhibited remarkable variability, ranging from 3% to 50%.
Intriguingly, at four of the sites, the incidence of Bs was significantly lower (ranging from 3% to 13%) compared to the average frequencies observed in natural populations. Conversely, at one site, the frequency soared to an exceptionally high 50%. Since all studied localities are at short distances from each other, climatic factors could be considered as the same. However, the studied sites differ in size and the level of adaptation to people’s urban life. In a bid to elucidate the driving factors behind frequency variation, we developed a model simulating the influence of genetic drift, the predominant evolutionary force in small, isolated populations.
Conclusions
Beyond investigations focused on the molecular structure and origin of B chromosomes, it becomes evident that a comprehensive understanding necessitates population-level research, shedding light on
the intricate mechanisms governing their persistence across diverse environments.",
publisher = "Springer Nature",
journal = "Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia",
title = "Variation in the frequency of B chromosomes in small isolated populations of Apodemus flavicollis",
doi = "10.1186/s12919-023-00284-9",
pages = "33"
}

Blagojević, J., Malkov, S., Rajičić, M., Rončević, A., Budinski, I., Bajić, B., Miljević, M.,& Vujošević, M.. (2023). Variation in the frequency of B chromosomes in small isolated populations of Apodemus flavicollis. in Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia
Springer Nature., 33.
https://doi.org/10.1186/s12919-023-00284-9

Blagojević J, Malkov S, Rajičić M, Rončević A, Budinski I, Bajić B, Miljević M, Vujošević M. Variation in the frequency of B chromosomes in small isolated populations of Apodemus flavicollis. in Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia. 2023;:33.
doi:10.1186/s12919-023-00284-9 .

Blagojević, Jelena, Malkov, Saša, Rajičić, Marija, Rončević, Aleksa, Budinski, Ivana, Bajić, Branka, Miljević, Milan, Vujošević, Mladen, "Variation in the frequency of B chromosomes in small isolated populations of Apodemus flavicollis" in Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia (2023):33,
https://doi.org/10.1186/s12919-023-00284-9 . .

TY  - CONF
AU  - Rajičić, Marija
AU  - Miljević, Milan
AU  - Bajić, Branka
AU  - Budinski, Ivana
AU  - Rončević, Aleksa
AU  - Vujošević, Mladen
AU  - Blagojević, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6282
AB  - B chromosomes (Bs) are additional elements to the standard karyotype that are not required for
normal growth and development. Bs are predominantly derived from chromosomes of the
standard chromosome set. In some species, these supernumerary chromosomes are passed on to
the next generation more frequently than would be expected under Mendel's law of segregation.
Although the molecular mechanism is unknown, this chromosome drive can occur during
premeiotic, meiotic, or postmeiotic division. Inheritance of B chromosomes across generations is
unknown in many species, including Apodemus flavicollis. The standard karyotype of this
species (2n=48) may contain up to eight B chromosomes, but animals with 1B are most
common. Animals with Bs are present in all studied populations of A. flavicollis in Serbia with
different frequencies (0.10-0.64).
Here we studied the transmission rate of B chromosomes in captive-bred pairs of A. flavicollis.
Juveniles were selected for sex and Bs markers, all of which were determined by PCR. In
addition, primary cell cultures and chromosome preparations were performed for animals with
positive B markers to select animals with 1B. In this way, we were able to form two
combinations of breeding pairs: four pairs with 1B females and 0B males and three pairs with 0B
females and 1B males.
In the pairs where the female had 1B and the male 0B, we obtained a total of 20 offspring, 14 of
which were carriers of the B chromosome (13 with 1B and one animal was a mosaic with 0-2Bs
in the cells). In pairs where males were carriers of 1B and females of 0B, we obtained 10
offspring, of which five animals were carriers of the B chromosome (4 with 1B and one animal
was a mosaic with 0-1B).
In our previous studies of more than forty natural populations, the frequencies of animals with
Bs were the same in males and females. Also, previous studies of meiotic segregation in males
showed that there was no accumulation of Bs, suggesting a general lack of meiotic drive. Herein,
for the first time, we found deviations from Mendel's law of segregation in the transmission of
Bs by the female sex. Our preliminary results suggest the possible presence of a meiotic drive in
the female sex of A. flavicollis, in which ¾ of the offspring inherit B from the mother, whereas in
the male sex B is inherited according to Mendel's law of segregation. However, the small number
of crossed pairs of animals and the small number of litters in the present study indicate the need
to continue this experiment to address the issue of Bs transmission across generations in A.
flavicollis
PB  - Novosibirsk: Institute of Molecular and Cellular Biology SB RAS
C3  - Abstracts: International conference: Chromosome 2023; 2023 Sep 5-10; Novosibirsk, Russia
T1  - B chromosome inheritance in Apodemus flavicollis
DO  - 10.25205/978-5-4437-1514-8
SP  - 42
ER  - 

@conference{
author = "Rajičić, Marija and Miljević, Milan and Bajić, Branka and Budinski, Ivana and Rončević, Aleksa and Vujošević, Mladen and Blagojević, Jelena",
year = "2023",
abstract = "B chromosomes (Bs) are additional elements to the standard karyotype that are not required for
normal growth and development. Bs are predominantly derived from chromosomes of the
standard chromosome set. In some species, these supernumerary chromosomes are passed on to
the next generation more frequently than would be expected under Mendel's law of segregation.
Although the molecular mechanism is unknown, this chromosome drive can occur during
premeiotic, meiotic, or postmeiotic division. Inheritance of B chromosomes across generations is
unknown in many species, including Apodemus flavicollis. The standard karyotype of this
species (2n=48) may contain up to eight B chromosomes, but animals with 1B are most
common. Animals with Bs are present in all studied populations of A. flavicollis in Serbia with
different frequencies (0.10-0.64).
Here we studied the transmission rate of B chromosomes in captive-bred pairs of A. flavicollis.
Juveniles were selected for sex and Bs markers, all of which were determined by PCR. In
addition, primary cell cultures and chromosome preparations were performed for animals with
positive B markers to select animals with 1B. In this way, we were able to form two
combinations of breeding pairs: four pairs with 1B females and 0B males and three pairs with 0B
females and 1B males.
In the pairs where the female had 1B and the male 0B, we obtained a total of 20 offspring, 14 of
which were carriers of the B chromosome (13 with 1B and one animal was a mosaic with 0-2Bs
in the cells). In pairs where males were carriers of 1B and females of 0B, we obtained 10
offspring, of which five animals were carriers of the B chromosome (4 with 1B and one animal
was a mosaic with 0-1B).
In our previous studies of more than forty natural populations, the frequencies of animals with
Bs were the same in males and females. Also, previous studies of meiotic segregation in males
showed that there was no accumulation of Bs, suggesting a general lack of meiotic drive. Herein,
for the first time, we found deviations from Mendel's law of segregation in the transmission of
Bs by the female sex. Our preliminary results suggest the possible presence of a meiotic drive in
the female sex of A. flavicollis, in which ¾ of the offspring inherit B from the mother, whereas in
the male sex B is inherited according to Mendel's law of segregation. However, the small number
of crossed pairs of animals and the small number of litters in the present study indicate the need
to continue this experiment to address the issue of Bs transmission across generations in A.
flavicollis",
publisher = "Novosibirsk: Institute of Molecular and Cellular Biology SB RAS",
journal = "Abstracts: International conference: Chromosome 2023; 2023 Sep 5-10; Novosibirsk, Russia",
title = "B chromosome inheritance in Apodemus flavicollis",
doi = "10.25205/978-5-4437-1514-8",
pages = "42"
}

Rajičić, M., Miljević, M., Bajić, B., Budinski, I., Rončević, A., Vujošević, M.,& Blagojević, J.. (2023). B chromosome inheritance in Apodemus flavicollis. in Abstracts: International conference: Chromosome 2023; 2023 Sep 5-10; Novosibirsk, Russia
Novosibirsk: Institute of Molecular and Cellular Biology SB RAS., 42.
https://doi.org/10.25205/978-5-4437-1514-8

Rajičić M, Miljević M, Bajić B, Budinski I, Rončević A, Vujošević M, Blagojević J. B chromosome inheritance in Apodemus flavicollis. in Abstracts: International conference: Chromosome 2023; 2023 Sep 5-10; Novosibirsk, Russia. 2023;:42.
doi:10.25205/978-5-4437-1514-8 .

Rajičić, Marija, Miljević, Milan, Bajić, Branka, Budinski, Ivana, Rončević, Aleksa, Vujošević, Mladen, Blagojević, Jelena, "B chromosome inheritance in Apodemus flavicollis" in Abstracts: International conference: Chromosome 2023; 2023 Sep 5-10; Novosibirsk, Russia (2023):42,
https://doi.org/10.25205/978-5-4437-1514-8 . .

TY  - CONF
AU  - Blagojević, Jelena
AU  - Rajičić, Marija
AU  - Adnađević, Tanja
AU  - Budinski, Ivana
AU  - Bajić, Branka
AU  - Miljević, Milan
AU  - Rončević, Aleksa
AU  - Vujošević, Mladen
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5775
AB  - B chromosomes (Bs), or supernumerary chromosomes, are highly polymorphic special chromosome group. Among rodents, 37 species harbour individuals with Bs in their populations. Genus Apodemus is specific, with even six species having Bs. Furthermore, long-term studies in natural populations of the yellow-necked wood mouse Apodemus flavicollis showed that one-third of individuals carry Bs on average. In more than 40 natural populations we analysed in Serbia, the frequency of animals with Bs ranged from 0.11 to 0.63 and correlated negatively with average temperature and positively with attitude. In general, non-Mendelian transmission and frequent heterochromatic composition are characteristics that have classified Bs as selfish genetic elements in the past. However, there are increasing confirmations of active genes in them, which opens the discussion on their adaptive significance. We aim to investigate how urbanisation influences the frequency of Bs in this species. Urbanisation is rapidly changing natural habitats and affecting animal community composition, genetic diversity, and the interrelationship between populations of native species. Habitat fragmentation is the leading course of genetic diversity loss. We analysed the frequency of animals with Bs in four managed city forests on the territory of Belgrade, the capital of Serbia, characterised by different sizes and degrees of isolation. Our study revealed that the frequency of animals with Bs varies significantly among studied sites, from 0.04 to 0.50. We assume that, besides other factors, genetic drift plays a significant role in obtained differences.
PB  - Valladolid: Escuela Técnica Superior de Ingenierías Agrarias, Universidad de Valladolid
C3  - Book of Abstracts: 17th Rodens et spatium; 2023 May 22-26; Valladolid, Spain
T1  - B chromosomes in urban populations of yellow-necked wood mice, Apodemus flavicolis (Mammalia, Rodentia)
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5775
ER  - 

@conference{
editor = "Herrero, Cófreces, Larena, Silvia, Larena, Luque, José, Juan",
author = "Blagojević, Jelena and Rajičić, Marija and Adnađević, Tanja and Budinski, Ivana and Bajić, Branka and Miljević, Milan and Rončević, Aleksa and Vujošević, Mladen",
year = "2023",
abstract = "B chromosomes (Bs), or supernumerary chromosomes, are highly polymorphic special chromosome group. Among rodents, 37 species harbour individuals with Bs in their populations. Genus Apodemus is specific, with even six species having Bs. Furthermore, long-term studies in natural populations of the yellow-necked wood mouse Apodemus flavicollis showed that one-third of individuals carry Bs on average. In more than 40 natural populations we analysed in Serbia, the frequency of animals with Bs ranged from 0.11 to 0.63 and correlated negatively with average temperature and positively with attitude. In general, non-Mendelian transmission and frequent heterochromatic composition are characteristics that have classified Bs as selfish genetic elements in the past. However, there are increasing confirmations of active genes in them, which opens the discussion on their adaptive significance. We aim to investigate how urbanisation influences the frequency of Bs in this species. Urbanisation is rapidly changing natural habitats and affecting animal community composition, genetic diversity, and the interrelationship between populations of native species. Habitat fragmentation is the leading course of genetic diversity loss. We analysed the frequency of animals with Bs in four managed city forests on the territory of Belgrade, the capital of Serbia, characterised by different sizes and degrees of isolation. Our study revealed that the frequency of animals with Bs varies significantly among studied sites, from 0.04 to 0.50. We assume that, besides other factors, genetic drift plays a significant role in obtained differences.",
publisher = "Valladolid: Escuela Técnica Superior de Ingenierías Agrarias, Universidad de Valladolid",
journal = "Book of Abstracts: 17th Rodens et spatium; 2023 May 22-26; Valladolid, Spain",
title = "B chromosomes in urban populations of yellow-necked wood mice, Apodemus flavicolis (Mammalia, Rodentia)",
pages = "30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5775"
}

Herrero, C., Larena, S., Larena, L., José, J., Blagojević, J., Rajičić, M., Adnađević, T., Budinski, I., Bajić, B., Miljević, M., Rončević, A.,& Vujošević, M.. (2023). B chromosomes in urban populations of yellow-necked wood mice, Apodemus flavicolis (Mammalia, Rodentia). in Book of Abstracts: 17th Rodens et spatium; 2023 May 22-26; Valladolid, Spain
Valladolid: Escuela Técnica Superior de Ingenierías Agrarias, Universidad de Valladolid., 30.
https://hdl.handle.net/21.15107/rcub_ibiss_5775

Herrero C, Larena S, Larena L, José J, Blagojević J, Rajičić M, Adnađević T, Budinski I, Bajić B, Miljević M, Rončević A, Vujošević M. B chromosomes in urban populations of yellow-necked wood mice, Apodemus flavicolis (Mammalia, Rodentia). in Book of Abstracts: 17th Rodens et spatium; 2023 May 22-26; Valladolid, Spain. 2023;:30.
https://hdl.handle.net/21.15107/rcub_ibiss_5775 .

Herrero, Cófreces, Larena, Silvia, Larena, Luque, José, Juan, Blagojević, Jelena, Rajičić, Marija, Adnađević, Tanja, Budinski, Ivana, Bajić, Branka, Miljević, Milan, Rončević, Aleksa, Vujošević, Mladen, "B chromosomes in urban populations of yellow-necked wood mice, Apodemus flavicolis (Mammalia, Rodentia)" in Book of Abstracts: 17th Rodens et spatium; 2023 May 22-26; Valladolid, Spain (2023):30,
https://hdl.handle.net/21.15107/rcub_ibiss_5775 .

TY  - JOUR
AU  - Rajičić, Marija
AU  - Budinski, Ivana
AU  - Miljević, Milan
AU  - Bajić, Branka
AU  - Paunović, Milan
AU  - Vujošević, Mladen
AU  - Blagojević, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5242
AB  - B chromosomes (Bs) are supernumerary to the standard chromosome set, from which they prevalently
derive. Variation in numbers both among individuals or populations and among cells within individuals
is their constant feature. Leisler’s bat Nyctalus leisleri (Kuhl, 1817) is one of only four species of Chiroptera
with detected Bs. Four males of N. leisleri were collected from two localities on the territory of Serbia
and cytogenetically analysed. All animals had Bs with interindividual variability ranging from two to
five heterochromatic micro Bs. The highest number of Bs was detected in this species. Among mammals,
Rodentia and Chiroptera are orders with the largest number of species, but Bs frequently appear in rodents
and rarely in chiropterans. Possible explanations for this difference are offered.
PB  - Pensoft Publishers
T2  - Comparative Cytogenetics
T1  - The new highest number of B chromosomes (Bs) in Leisler's bat Nyctalus leisleri (Kuhl, 1817)
IS  - 3
VL  - 16
DO  - 10.3897/CompCytogen.v16i3.89911
SP  - 173
EP  - 184
ER  - 

@article{
author = "Rajičić, Marija and Budinski, Ivana and Miljević, Milan and Bajić, Branka and Paunović, Milan and Vujošević, Mladen and Blagojević, Jelena",
year = "2022",
abstract = "B chromosomes (Bs) are supernumerary to the standard chromosome set, from which they prevalently
derive. Variation in numbers both among individuals or populations and among cells within individuals
is their constant feature. Leisler’s bat Nyctalus leisleri (Kuhl, 1817) is one of only four species of Chiroptera
with detected Bs. Four males of N. leisleri were collected from two localities on the territory of Serbia
and cytogenetically analysed. All animals had Bs with interindividual variability ranging from two to
five heterochromatic micro Bs. The highest number of Bs was detected in this species. Among mammals,
Rodentia and Chiroptera are orders with the largest number of species, but Bs frequently appear in rodents
and rarely in chiropterans. Possible explanations for this difference are offered.",
publisher = "Pensoft Publishers",
journal = "Comparative Cytogenetics",
title = "The new highest number of B chromosomes (Bs) in Leisler's bat Nyctalus leisleri (Kuhl, 1817)",
number = "3",
volume = "16",
doi = "10.3897/CompCytogen.v16i3.89911",
pages = "173-184"
}

Rajičić, M., Budinski, I., Miljević, M., Bajić, B., Paunović, M., Vujošević, M.,& Blagojević, J.. (2022). The new highest number of B chromosomes (Bs) in Leisler's bat Nyctalus leisleri (Kuhl, 1817). in Comparative Cytogenetics
Pensoft Publishers., 16(3), 173-184.
https://doi.org/10.3897/CompCytogen.v16i3.89911

Rajičić M, Budinski I, Miljević M, Bajić B, Paunović M, Vujošević M, Blagojević J. The new highest number of B chromosomes (Bs) in Leisler's bat Nyctalus leisleri (Kuhl, 1817). in Comparative Cytogenetics. 2022;16(3):173-184.
doi:10.3897/CompCytogen.v16i3.89911 .

Rajičić, Marija, Budinski, Ivana, Miljević, Milan, Bajić, Branka, Paunović, Milan, Vujošević, Mladen, Blagojević, Jelena, "The new highest number of B chromosomes (Bs) in Leisler's bat Nyctalus leisleri (Kuhl, 1817)" in Comparative Cytogenetics, 16, no. 3 (2022):173-184,
https://doi.org/10.3897/CompCytogen.v16i3.89911 . .

TY  - JOUR
AU  - Rajičić, Marija
AU  - Makunin, Alexey
AU  - Adnađević, Tanja
AU  - Trifonov, Vladimir
AU  - Vujošević, Mladen
AU  - Blagojević, Jelena
PY  - 2022
UR  - https://www.mdpi.com/2075-1729/12/1/50
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4764
AB  - B chromosomes (Bs) are highly polymorphic additional chromosomes in the genomes of many species. Due to the dispensability of Bs and the lack of noticeable phenotypic effects in their carriers, they were considered genetically inert for a long time. Recent studies on Bs in Apodemus flavicollis revealed their genetic composition, potential origin, and spatial organization in the interphase nucleus. Surprisingly, the genetic content of Bs in this species is preserved in all studied samples, even in geographically distinct populations, indicating its biological importance. Using RT-PCR we studied the transcription activity of three genes (Rraga, Haus6, and Cenpe) previously identified on Bs in A. flavicollis. We analysed mRNA isolated from spleen tissues of 34 animals harboring different numbers of Bs (0–3).The products of transcriptional activity of the analysed sequences differ in individuals with and without Bs. We recorded B-genes and/or genes from the standard genome in the presence of Bs, showing sex-dependent higher levels of transcriptional activity. Furthermore, the transcriptional activity of Cenpe varied with the age of the animals differently in the group with and without Bs. With aging, the amount of product was only found to significantly decrease in B carriers. The potential biological significance of all these differences is discussed in the paper.
PB  - Basel: MDPI
T2  - Life
T1  - B Chromosomes’ Sequences in Yellow-Necked Mice Apodemus flavicollis—Exploring the Transcription
IS  - 1
VL  - 12
DO  - 10.3390/life12010050
SP  - 50
ER  - 

@article{
author = "Rajičić, Marija and Makunin, Alexey and Adnađević, Tanja and Trifonov, Vladimir and Vujošević, Mladen and Blagojević, Jelena",
year = "2022",
abstract = "B chromosomes (Bs) are highly polymorphic additional chromosomes in the genomes of many species. Due to the dispensability of Bs and the lack of noticeable phenotypic effects in their carriers, they were considered genetically inert for a long time. Recent studies on Bs in Apodemus flavicollis revealed their genetic composition, potential origin, and spatial organization in the interphase nucleus. Surprisingly, the genetic content of Bs in this species is preserved in all studied samples, even in geographically distinct populations, indicating its biological importance. Using RT-PCR we studied the transcription activity of three genes (Rraga, Haus6, and Cenpe) previously identified on Bs in A. flavicollis. We analysed mRNA isolated from spleen tissues of 34 animals harboring different numbers of Bs (0–3).The products of transcriptional activity of the analysed sequences differ in individuals with and without Bs. We recorded B-genes and/or genes from the standard genome in the presence of Bs, showing sex-dependent higher levels of transcriptional activity. Furthermore, the transcriptional activity of Cenpe varied with the age of the animals differently in the group with and without Bs. With aging, the amount of product was only found to significantly decrease in B carriers. The potential biological significance of all these differences is discussed in the paper.",
publisher = "Basel: MDPI",
journal = "Life",
title = "B Chromosomes’ Sequences in Yellow-Necked Mice Apodemus flavicollis—Exploring the Transcription",
number = "1",
volume = "12",
doi = "10.3390/life12010050",
pages = "50"
}

Rajičić, M., Makunin, A., Adnađević, T., Trifonov, V., Vujošević, M.,& Blagojević, J.. (2022). B Chromosomes’ Sequences in Yellow-Necked Mice Apodemus flavicollis—Exploring the Transcription. in Life
Basel: MDPI., 12(1), 50.
https://doi.org/10.3390/life12010050

Rajičić M, Makunin A, Adnađević T, Trifonov V, Vujošević M, Blagojević J. B Chromosomes’ Sequences in Yellow-Necked Mice Apodemus flavicollis—Exploring the Transcription. in Life. 2022;12(1):50.
doi:10.3390/life12010050 .

Rajičić, Marija, Makunin, Alexey, Adnađević, Tanja, Trifonov, Vladimir, Vujošević, Mladen, Blagojević, Jelena, "B Chromosomes’ Sequences in Yellow-Necked Mice Apodemus flavicollis—Exploring the Transcription" in Life, 12, no. 1 (2022):50,
https://doi.org/10.3390/life12010050 . .

TY  - JOUR
AU  - Karamysheva, Tatyana
AU  - Romanenko, Svetlana
AU  - Makunin, Alexey
AU  - Rajičić, Marija
AU  - Bogdanov, Alexey
AU  - Trifonov, Vladimir
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
AU  - Orishchenko, Konstantin
AU  - Rubtsov, Nikolay
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/34359988
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8305704
UR  - https://www.mdpi.com/2073-4409/10/7/1819
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4453
AB  - The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that heterochromatic compartments are not randomly localized in a nucleus. The purpose of this work was to study the organization and three-dimensional spatial arrangement of Bs in the interphase nucleus. Using microdissection of Bs and autosome centromeric heterochromatic regions of the yellow-necked mouse (Apodemus flavicollis) we obtained DNA probes for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained here B-specific DNA probes and autosomal C-positive pericentromeric region-specific probes further corroborated the previously stated hypothesis about the pseudoautosomal origin of the additional chromosomes of this species. Analysis of the spatial organization of the Bs demonstrated the peripheral location of B-specific chromatin within the interphase nucleus and feasible contact with the nuclear envelope (similarly to pericentromeric regions of autosomes and sex chromosomes). It is assumed that such interaction is essential for the regulation of nuclear architecture. It also points out that Bs may follow the same mechanism as sex chromosomes to avoid a meiotic checkpoint.
T2  - Cells
T1  - New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis
IS  - 7
VL  - 10
DO  - 10.3390/cells10071819
SP  - 1819
ER  - 

@article{
author = "Karamysheva, Tatyana and Romanenko, Svetlana and Makunin, Alexey and Rajičić, Marija and Bogdanov, Alexey and Trifonov, Vladimir and Blagojević, Jelena and Vujošević, Mladen and Orishchenko, Konstantin and Rubtsov, Nikolay",
year = "2021",
abstract = "The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that heterochromatic compartments are not randomly localized in a nucleus. The purpose of this work was to study the organization and three-dimensional spatial arrangement of Bs in the interphase nucleus. Using microdissection of Bs and autosome centromeric heterochromatic regions of the yellow-necked mouse (Apodemus flavicollis) we obtained DNA probes for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained here B-specific DNA probes and autosomal C-positive pericentromeric region-specific probes further corroborated the previously stated hypothesis about the pseudoautosomal origin of the additional chromosomes of this species. Analysis of the spatial organization of the Bs demonstrated the peripheral location of B-specific chromatin within the interphase nucleus and feasible contact with the nuclear envelope (similarly to pericentromeric regions of autosomes and sex chromosomes). It is assumed that such interaction is essential for the regulation of nuclear architecture. It also points out that Bs may follow the same mechanism as sex chromosomes to avoid a meiotic checkpoint.",
journal = "Cells",
title = "New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis",
number = "7",
volume = "10",
doi = "10.3390/cells10071819",
pages = "1819"
}

Karamysheva, T., Romanenko, S., Makunin, A., Rajičić, M., Bogdanov, A., Trifonov, V., Blagojević, J., Vujošević, M., Orishchenko, K.,& Rubtsov, N.. (2021). New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis. in Cells, 10(7), 1819.
https://doi.org/10.3390/cells10071819

Karamysheva T, Romanenko S, Makunin A, Rajičić M, Bogdanov A, Trifonov V, Blagojević J, Vujošević M, Orishchenko K, Rubtsov N. New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis. in Cells. 2021;10(7):1819.
doi:10.3390/cells10071819 .

Karamysheva, Tatyana, Romanenko, Svetlana, Makunin, Alexey, Rajičić, Marija, Bogdanov, Alexey, Trifonov, Vladimir, Blagojević, Jelena, Vujošević, Mladen, Orishchenko, Konstantin, Rubtsov, Nikolay, "New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis" in Cells, 10, no. 7 (2021):1819,
https://doi.org/10.3390/cells10071819 . .

TY  - JOUR
AU  - Jojić, Vida
AU  - Čabrilo, Borislav
AU  - Bjelić-Čabrilo, Olivera
AU  - Jovanović, Vladimir
AU  - Budinski, Ivana
AU  - Vujošević, Mladen
AU  - Blagojević, Jelena
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4645
AB  - Background:  Mammalian mandible and cranium are well‑established model systems for studying canalization 
and developmental stability (DS) as two elements of developmental homeostasis. Nematode infections are usually 
acquired in early life and increase in intensity with age, while canalization and DS of rodent skulls could vary through 
late postnatal ontogeny. We aimed to estimate magnitudes and describe patterns of mandibular and cranial canaliza‑
tion and DS related to age and parasite intensity (diversity) in adult yellow‑necked mice (Apodemus favicollis).
Results:  We found the absence of age‑related changes in the levels of canalization for mandibular and cranial size 
and DS for mandibular size. However, individual measures of mandibular and cranial shape variance increased, while 
individual measures of mandibular shape fuctuating asymmetry (FA) decreased with age. We detected mandibular 
and cranial shape changes during postnatal ontogeny, but revealed no age‑related dynamics of their covariance 
structure among and within individuals. Categories regarding parasitism difered in the level of canalization for cranial 
size and the level of DS for cranial shape. We observed diferences in age‑related dynamics of the level of canalization 
between non‑parasitized and parasitized animals, as well as between yellow‑necked mice parasitized by diferent 
number of nematode species. Likewise, individual measures of mandibular and cranial shape FA decreased with age 
for the mandible in the less parasitized category and increased for the cranium in the most parasitized category.
Conclusions:  Our age‑related results partly agree with previous fndings. However, no rodent study so far has 
explored age‑related changes in the magnitude of FA for mandibular size or mandibular and cranial FA covariance 
structure. This is the frst study dealing with the nematode parasitism‑related canalization and DS in rodents. We 
showed that nematode parasitism does not afect mandibular and cranial shape variation and covariance structure 
among and within individuals. However, parasite intensity (diversity) is related to ontogenetic dynamics of the levels 
of canalization and DS. Overall, additional studies on animals from natural populations are required before drawing 
some general conclusions.
PB  - BioMed Central Ltd. Part of Springer Nature.
T2  - Frontiers in Zoology
T1  - Canalization and developmental stability  of the yellow-necked mouse (Apodemus  favicollis) mandible and cranium related to age  and nematode parasitism
VL  - 18
DO  - 10.1186/s12983-021-00439-4
SP  - 55
ER  - 

@article{
author = "Jojić, Vida and Čabrilo, Borislav and Bjelić-Čabrilo, Olivera and Jovanović, Vladimir and Budinski, Ivana and Vujošević, Mladen and Blagojević, Jelena",
year = "2021",
abstract = "Background:  Mammalian mandible and cranium are well‑established model systems for studying canalization 
and developmental stability (DS) as two elements of developmental homeostasis. Nematode infections are usually 
acquired in early life and increase in intensity with age, while canalization and DS of rodent skulls could vary through 
late postnatal ontogeny. We aimed to estimate magnitudes and describe patterns of mandibular and cranial canaliza‑
tion and DS related to age and parasite intensity (diversity) in adult yellow‑necked mice (Apodemus favicollis).
Results:  We found the absence of age‑related changes in the levels of canalization for mandibular and cranial size 
and DS for mandibular size. However, individual measures of mandibular and cranial shape variance increased, while 
individual measures of mandibular shape fuctuating asymmetry (FA) decreased with age. We detected mandibular 
and cranial shape changes during postnatal ontogeny, but revealed no age‑related dynamics of their covariance 
structure among and within individuals. Categories regarding parasitism difered in the level of canalization for cranial 
size and the level of DS for cranial shape. We observed diferences in age‑related dynamics of the level of canalization 
between non‑parasitized and parasitized animals, as well as between yellow‑necked mice parasitized by diferent 
number of nematode species. Likewise, individual measures of mandibular and cranial shape FA decreased with age 
for the mandible in the less parasitized category and increased for the cranium in the most parasitized category.
Conclusions:  Our age‑related results partly agree with previous fndings. However, no rodent study so far has 
explored age‑related changes in the magnitude of FA for mandibular size or mandibular and cranial FA covariance 
structure. This is the frst study dealing with the nematode parasitism‑related canalization and DS in rodents. We 
showed that nematode parasitism does not afect mandibular and cranial shape variation and covariance structure 
among and within individuals. However, parasite intensity (diversity) is related to ontogenetic dynamics of the levels 
of canalization and DS. Overall, additional studies on animals from natural populations are required before drawing 
some general conclusions.",
publisher = "BioMed Central Ltd. Part of Springer Nature.",
journal = "Frontiers in Zoology",
title = "Canalization and developmental stability  of the yellow-necked mouse (Apodemus  favicollis) mandible and cranium related to age  and nematode parasitism",
volume = "18",
doi = "10.1186/s12983-021-00439-4",
pages = "55"
}

Jojić, V., Čabrilo, B., Bjelić-Čabrilo, O., Jovanović, V., Budinski, I., Vujošević, M.,& Blagojević, J.. (2021). Canalization and developmental stability  of the yellow-necked mouse (Apodemus  favicollis) mandible and cranium related to age  and nematode parasitism. in Frontiers in Zoology
BioMed Central Ltd. Part of Springer Nature.., 18, 55.
https://doi.org/10.1186/s12983-021-00439-4

Jojić V, Čabrilo B, Bjelić-Čabrilo O, Jovanović V, Budinski I, Vujošević M, Blagojević J. Canalization and developmental stability  of the yellow-necked mouse (Apodemus  favicollis) mandible and cranium related to age  and nematode parasitism. in Frontiers in Zoology. 2021;18:55.
doi:10.1186/s12983-021-00439-4 .

Jojić, Vida, Čabrilo, Borislav, Bjelić-Čabrilo, Olivera, Jovanović, Vladimir, Budinski, Ivana, Vujošević, Mladen, Blagojević, Jelena, "Canalization and developmental stability  of the yellow-necked mouse (Apodemus  favicollis) mandible and cranium related to age  and nematode parasitism" in Frontiers in Zoology, 18 (2021):55,
https://doi.org/10.1186/s12983-021-00439-4 . .

TY  - CONF
AU  - Čabrilo, Borislav
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
AU  - Miljević, Milan
AU  - Tošić, Božana
AU  - Bjelić Čabrilo, Olivera
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4640
AB  - Background. B chromosomes are supernumerary chromosomes that have been discovered in over 1000 eukaryote species. They show remarkable variaton in structure and behaviour, their only common trait being their conditonal dispensability. The influence of these genetc elements on their carriers is stll debated, with the two most accepted models of their maintenance being the parasitc and the heterotc model.
Objectves. The purpose of this study was to investgate the possible influence of B chromosomes on quanttatve characteristcs of intestnal nematode infecton in the yellow-necked mouse (Apodemus flavicollis).
Material and Methods. A total of 305 mice were sampled across 18 localites on the territory of Serbia over five years. Every individual with more than 48 chromosomes was considered to have Bs. Afer dissecton, intestnal nematodes were extracted and identfed. An analysis of Bs influence on parasite prevalence and abundance was conducted, using the exact unconditonal test and generalized linear modelling respectvely.
Results. Nine intestnal nematode species were detected in the host sample. In the total host sample, both prevalence and abundance of intestnal nematodes were greater in Bs carriers. Two nematode species, Aspiculuris tetraptera and Mastophorus muris, had signifcantly higher prevalence in the B+ subset of the host sample. Similarly, B chromosomes explained a signifcant proporton of abundance variaton of the nematode species Syphacia frederici, with its abundance greater in Bs carriers.
Conclusion. While the results of this study show a possible connecton between Bs presence and higher prevalence and abundance of intestnal nematodes, this fnding is purely correlatonal. Previously published data indicate a complex effect of B chromosomes on host characteristcs and survival through various molecular pathways. Bs carriers may reap the benefts of increased survival prospects as a by-product of the selfsh behaviour of the chromosomes themselves, blurring the line between the parasitc and heterotc model.
PB  - Belgrade: Serbian Society of Parasitology
C3  - Programme & Abstract book: 13th European Multicolloquium of Parasitology; 2021 Oct 12-16; Belgrade, Serbia
T1  - Possible influence of B chromosomes on the prevalence and abundance of intestinal nematode parasites of the yellow-necked mouse (Apodemus flavicollis)
SP  - 261
EP  - 262
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4640
ER  - 

@conference{
author = "Čabrilo, Borislav and Blagojević, Jelena and Vujošević, Mladen and Miljević, Milan and Tošić, Božana and Bjelić Čabrilo, Olivera",
year = "2021",
abstract = "Background. B chromosomes are supernumerary chromosomes that have been discovered in over 1000 eukaryote species. They show remarkable variaton in structure and behaviour, their only common trait being their conditonal dispensability. The influence of these genetc elements on their carriers is stll debated, with the two most accepted models of their maintenance being the parasitc and the heterotc model.
Objectves. The purpose of this study was to investgate the possible influence of B chromosomes on quanttatve characteristcs of intestnal nematode infecton in the yellow-necked mouse (Apodemus flavicollis).
Material and Methods. A total of 305 mice were sampled across 18 localites on the territory of Serbia over five years. Every individual with more than 48 chromosomes was considered to have Bs. Afer dissecton, intestnal nematodes were extracted and identfed. An analysis of Bs influence on parasite prevalence and abundance was conducted, using the exact unconditonal test and generalized linear modelling respectvely.
Results. Nine intestnal nematode species were detected in the host sample. In the total host sample, both prevalence and abundance of intestnal nematodes were greater in Bs carriers. Two nematode species, Aspiculuris tetraptera and Mastophorus muris, had signifcantly higher prevalence in the B+ subset of the host sample. Similarly, B chromosomes explained a signifcant proporton of abundance variaton of the nematode species Syphacia frederici, with its abundance greater in Bs carriers.
Conclusion. While the results of this study show a possible connecton between Bs presence and higher prevalence and abundance of intestnal nematodes, this fnding is purely correlatonal. Previously published data indicate a complex effect of B chromosomes on host characteristcs and survival through various molecular pathways. Bs carriers may reap the benefts of increased survival prospects as a by-product of the selfsh behaviour of the chromosomes themselves, blurring the line between the parasitc and heterotc model.",
publisher = "Belgrade: Serbian Society of Parasitology",
journal = "Programme & Abstract book: 13th European Multicolloquium of Parasitology; 2021 Oct 12-16; Belgrade, Serbia",
title = "Possible influence of B chromosomes on the prevalence and abundance of intestinal nematode parasites of the yellow-necked mouse (Apodemus flavicollis)",
pages = "261-262",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4640"
}

Čabrilo, B., Blagojević, J., Vujošević, M., Miljević, M., Tošić, B.,& Bjelić Čabrilo, O.. (2021). Possible influence of B chromosomes on the prevalence and abundance of intestinal nematode parasites of the yellow-necked mouse (Apodemus flavicollis). in Programme & Abstract book: 13th European Multicolloquium of Parasitology; 2021 Oct 12-16; Belgrade, Serbia
Belgrade: Serbian Society of Parasitology., 261-262.
https://hdl.handle.net/21.15107/rcub_ibiss_4640

Čabrilo B, Blagojević J, Vujošević M, Miljević M, Tošić B, Bjelić Čabrilo O. Possible influence of B chromosomes on the prevalence and abundance of intestinal nematode parasites of the yellow-necked mouse (Apodemus flavicollis). in Programme & Abstract book: 13th European Multicolloquium of Parasitology; 2021 Oct 12-16; Belgrade, Serbia. 2021;:261-262.
https://hdl.handle.net/21.15107/rcub_ibiss_4640 .

Čabrilo, Borislav, Blagojević, Jelena, Vujošević, Mladen, Miljević, Milan, Tošić, Božana, Bjelić Čabrilo, Olivera, "Possible influence of B chromosomes on the prevalence and abundance of intestinal nematode parasites of the yellow-necked mouse (Apodemus flavicollis)" in Programme & Abstract book: 13th European Multicolloquium of Parasitology; 2021 Oct 12-16; Belgrade, Serbia (2021):261-262,
https://hdl.handle.net/21.15107/rcub_ibiss_4640 .

TY  - CONF
AU  - Budinski, Ivana
AU  - Jovanović, Vladimir
AU  - Bajić, Branka
AU  - Blagojević, Jelena
AU  - Rajičić, Marija
AU  - Paunović, Milan
AU  - Presetnik, Primož
AU  - Vujošević, Mladen
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4270
AB  - Large Mediterranean peninsulas of Europe like the Balkans, Italian and the Iberian Peninsula
have been recognized as Pleistocene glacial refugia for many temperate species. The aim of
this study was to investgate demographic history and genetc structure of Mediterranean
horseshoe bat populatons on the Balkan Peninsula. R. euryale is a cave-dwelling species
distributed throughout the Mediterranean region. We collected 82 samples from 20
localites in the Balkans and Italian Peninsula and analysed genetc diversity of mitochondrial
D-loop sequences. Our results revealed low nucleotde but high haplotype diversity, and 20
haplotypes were reported for the frst tme. Phylogenetc reconstructons showed that all
haplotypes obtained from both Peninsulas belong to the same lineage together with the
previously published samples from Turkey, southern France and North Africa. All haplotypes
from the current study represent single haplogroup and haplotype network had a star-like
topology that is indicatve of recent populaton expansion. Scenario of sudden demographic
expansion was also supported by shallow genetc differentaton and mismatch distributon
analysis, and we estmate that expansion within this lineage started afer the last glacial
maximum. We present the new data on genetc variaton in this species, and highlight the
importance of the Balkans in the demographic history of Mediterranean horseshoe bat. The
obtained results support the hypothesis that the Balkan Peninsula was a glacial refugium for
R. euryale during the Pleistocene.
PB  - Belgrade: Serbian Genetic Society
C3  - 6th Congress of the Serbian genetic society; 2019 Oct 13-17; Vrnjačka Banja, Serbia
T1  - Was the Balkan peninsula a glacial refugium for the Mediterranean horseshoe bat, Rhinolophus euryale (Blasius, 1853)
SP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4270
ER  - 

@conference{
author = "Budinski, Ivana and Jovanović, Vladimir and Bajić, Branka and Blagojević, Jelena and Rajičić, Marija and Paunović, Milan and Presetnik, Primož and Vujošević, Mladen",
year = "2019",
abstract = "Large Mediterranean peninsulas of Europe like the Balkans, Italian and the Iberian Peninsula
have been recognized as Pleistocene glacial refugia for many temperate species. The aim of
this study was to investgate demographic history and genetc structure of Mediterranean
horseshoe bat populatons on the Balkan Peninsula. R. euryale is a cave-dwelling species
distributed throughout the Mediterranean region. We collected 82 samples from 20
localites in the Balkans and Italian Peninsula and analysed genetc diversity of mitochondrial
D-loop sequences. Our results revealed low nucleotde but high haplotype diversity, and 20
haplotypes were reported for the frst tme. Phylogenetc reconstructons showed that all
haplotypes obtained from both Peninsulas belong to the same lineage together with the
previously published samples from Turkey, southern France and North Africa. All haplotypes
from the current study represent single haplogroup and haplotype network had a star-like
topology that is indicatve of recent populaton expansion. Scenario of sudden demographic
expansion was also supported by shallow genetc differentaton and mismatch distributon
analysis, and we estmate that expansion within this lineage started afer the last glacial
maximum. We present the new data on genetc variaton in this species, and highlight the
importance of the Balkans in the demographic history of Mediterranean horseshoe bat. The
obtained results support the hypothesis that the Balkan Peninsula was a glacial refugium for
R. euryale during the Pleistocene.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "6th Congress of the Serbian genetic society; 2019 Oct 13-17; Vrnjačka Banja, Serbia",
title = "Was the Balkan peninsula a glacial refugium for the Mediterranean horseshoe bat, Rhinolophus euryale (Blasius, 1853)",
pages = "151",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4270"
}

Budinski, I., Jovanović, V., Bajić, B., Blagojević, J., Rajičić, M., Paunović, M., Presetnik, P.,& Vujošević, M.. (2019). Was the Balkan peninsula a glacial refugium for the Mediterranean horseshoe bat, Rhinolophus euryale (Blasius, 1853). in 6th Congress of the Serbian genetic society; 2019 Oct 13-17; Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society., 151.
https://hdl.handle.net/21.15107/rcub_ibiss_4270

Budinski I, Jovanović V, Bajić B, Blagojević J, Rajičić M, Paunović M, Presetnik P, Vujošević M. Was the Balkan peninsula a glacial refugium for the Mediterranean horseshoe bat, Rhinolophus euryale (Blasius, 1853). in 6th Congress of the Serbian genetic society; 2019 Oct 13-17; Vrnjačka Banja, Serbia. 2019;:151.
https://hdl.handle.net/21.15107/rcub_ibiss_4270 .

Budinski, Ivana, Jovanović, Vladimir, Bajić, Branka, Blagojević, Jelena, Rajičić, Marija, Paunović, Milan, Presetnik, Primož, Vujošević, Mladen, "Was the Balkan peninsula a glacial refugium for the Mediterranean horseshoe bat, Rhinolophus euryale (Blasius, 1853)" in 6th Congress of the Serbian genetic society; 2019 Oct 13-17; Vrnjačka Banja, Serbia (2019):151,
https://hdl.handle.net/21.15107/rcub_ibiss_4270 .

TY  - JOUR
AU  - Budinski, Ivana
AU  - Jovanović, Vladimir
AU  - Bajić, Branka
AU  - Blagojević, Jelena
AU  - Rajičić, Marija
AU  - Paunović, Milan
AU  - Presetnik, Primož
AU  - Vujošević, Mladen
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3543
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4330
AB  - The Balkan Peninsula is identified as one of the major glacial refugia in Europe during the
Pleistocene, and it has served as a genetic source for post-glacial recolonization for many temperate
species. The aim of this study was to investigate the genetic diversity and phylogeographic patterns of the
Mediterranean horseshoe bat, Rhinolophus euryale Blasius 1853, on the Balkan Peninsula. We also
analyzed its demographic history and tested the hypothesis that this region was a glacial refugium for this
species. We collected 82 samples from 20 localities in the Balkans and Italy and sequenced the
mitochondrial D-loop region. Our results revealed low nucleotide but high haplotype diversity, with 20
out of 24 haplotypes reported for the first time. All Balkan and Italian samples belonged to a single
genetic clade in the phylogenetic reconstruction, where they clustered together with previously published
samples from Turkey, southern France and North Africa. The haplotype network had a star-like pattern
that is indicative of recent population expansion. Both mismatch distribution and shallow genetic
differentiation also supported the scenario of a sudden demographic expansion. We estimated that
expansion within this lineage commenced in the Late Pleistocene. We suggest that the Balkan Peninsula
was a glacial refugium for R. euryale.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Mitochondrial phylogeography of the Mediterranean horseshoe bat on the Balkan Peninsula
IS  - 4
VL  - 71
DO  - 10.2298/ABS190529059B
SP  - 767
EP  - 774
ER  - 

@article{
author = "Budinski, Ivana and Jovanović, Vladimir and Bajić, Branka and Blagojević, Jelena and Rajičić, Marija and Paunović, Milan and Presetnik, Primož and Vujošević, Mladen",
year = "2019",
abstract = "The Balkan Peninsula is identified as one of the major glacial refugia in Europe during the
Pleistocene, and it has served as a genetic source for post-glacial recolonization for many temperate
species. The aim of this study was to investigate the genetic diversity and phylogeographic patterns of the
Mediterranean horseshoe bat, Rhinolophus euryale Blasius 1853, on the Balkan Peninsula. We also
analyzed its demographic history and tested the hypothesis that this region was a glacial refugium for this
species. We collected 82 samples from 20 localities in the Balkans and Italy and sequenced the
mitochondrial D-loop region. Our results revealed low nucleotide but high haplotype diversity, with 20
out of 24 haplotypes reported for the first time. All Balkan and Italian samples belonged to a single
genetic clade in the phylogenetic reconstruction, where they clustered together with previously published
samples from Turkey, southern France and North Africa. The haplotype network had a star-like pattern
that is indicative of recent population expansion. Both mismatch distribution and shallow genetic
differentiation also supported the scenario of a sudden demographic expansion. We estimated that
expansion within this lineage commenced in the Late Pleistocene. We suggest that the Balkan Peninsula
was a glacial refugium for R. euryale.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Mitochondrial phylogeography of the Mediterranean horseshoe bat on the Balkan Peninsula",
number = "4",
volume = "71",
doi = "10.2298/ABS190529059B",
pages = "767-774"
}

Budinski, I., Jovanović, V., Bajić, B., Blagojević, J., Rajičić, M., Paunović, M., Presetnik, P.,& Vujošević, M.. (2019). Mitochondrial phylogeography of the Mediterranean horseshoe bat on the Balkan Peninsula. in Archives of Biological Sciences
Serbian Biological Society., 71(4), 767-774.
https://doi.org/10.2298/ABS190529059B

Budinski I, Jovanović V, Bajić B, Blagojević J, Rajičić M, Paunović M, Presetnik P, Vujošević M. Mitochondrial phylogeography of the Mediterranean horseshoe bat on the Balkan Peninsula. in Archives of Biological Sciences. 2019;71(4):767-774.
doi:10.2298/ABS190529059B .

Budinski, Ivana, Jovanović, Vladimir, Bajić, Branka, Blagojević, Jelena, Rajičić, Marija, Paunović, Milan, Presetnik, Primož, Vujošević, Mladen, "Mitochondrial phylogeography of the Mediterranean horseshoe bat on the Balkan Peninsula" in Archives of Biological Sciences, 71, no. 4 (2019):767-774,
https://doi.org/10.2298/ABS190529059B . .

TY  - JOUR
AU  - Blagojević, Jelena
AU  - Šekler, Milanko
AU  - Rajičić, Marija
AU  - Bajić, Branka
AU  - Budinski, Ivana
AU  - Jovanović, Vladimir
AU  - Adnađević, Tanja
AU  - Vidanović, Dejan
AU  - Matović, Kazimir
AU  - Vujošević, Mladen
PY  - 2019
UR  - https://www.akademiai.com/doi/10.1556/004.2019.035
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3479
AB  - The greatest epidemiological significance of leptospirosis in Europe comes from the fact that it is the most widespread zoonosis in the world. However, epizootiological data, especially information on maintenance hosts such as small wild mammals, are largely missing. To fill this gap in data in Serbia, we used RT-PCR for the detection of pathogenic Leptospira species and analysed 107 animals belonging to six species of small wild mammals (Apodemus agrarius, Apodemus flavicollis, Microtus arvalis, Myodes glareolus, Microtus subterraneus and Sorex araneus) collected from two localities. The animals from the first locality that was situated in a tourist area, were collected for four consecutive years (2014-2017). We found persistent incidence of infection from year to year ranging from 6.67% to 78.57%. The average frequency of infected animals was 33.3% with the highest frequency in 2014, the year characterised by a very high number of flooding days. All animals proved to be infected with pathogenic Leptospira species that were collected from the second locality situated in an agricultural area in a single year, 2014. The findings show a variable but constant presence of pathogenic Leptospira species in populations of small wild mammals in the studied areas, which indicates the need for constant monitoring.
T2  - Acta Veterinaria Hungarica
T1  - The prevalence of pathogenic forms of Leptospira in natural populations of small wild mammals in Serbia.
IS  - 3
VL  - 67
DO  - 10.1556/004.2019.035
SP  - 338
EP  - 346
ER  - 

@article{
author = "Blagojević, Jelena and Šekler, Milanko and Rajičić, Marija and Bajić, Branka and Budinski, Ivana and Jovanović, Vladimir and Adnađević, Tanja and Vidanović, Dejan and Matović, Kazimir and Vujošević, Mladen",
year = "2019",
abstract = "The greatest epidemiological significance of leptospirosis in Europe comes from the fact that it is the most widespread zoonosis in the world. However, epizootiological data, especially information on maintenance hosts such as small wild mammals, are largely missing. To fill this gap in data in Serbia, we used RT-PCR for the detection of pathogenic Leptospira species and analysed 107 animals belonging to six species of small wild mammals (Apodemus agrarius, Apodemus flavicollis, Microtus arvalis, Myodes glareolus, Microtus subterraneus and Sorex araneus) collected from two localities. The animals from the first locality that was situated in a tourist area, were collected for four consecutive years (2014-2017). We found persistent incidence of infection from year to year ranging from 6.67% to 78.57%. The average frequency of infected animals was 33.3% with the highest frequency in 2014, the year characterised by a very high number of flooding days. All animals proved to be infected with pathogenic Leptospira species that were collected from the second locality situated in an agricultural area in a single year, 2014. The findings show a variable but constant presence of pathogenic Leptospira species in populations of small wild mammals in the studied areas, which indicates the need for constant monitoring.",
journal = "Acta Veterinaria Hungarica",
title = "The prevalence of pathogenic forms of Leptospira in natural populations of small wild mammals in Serbia.",
number = "3",
volume = "67",
doi = "10.1556/004.2019.035",
pages = "338-346"
}

Blagojević, J., Šekler, M., Rajičić, M., Bajić, B., Budinski, I., Jovanović, V., Adnađević, T., Vidanović, D., Matović, K.,& Vujošević, M.. (2019). The prevalence of pathogenic forms of Leptospira in natural populations of small wild mammals in Serbia.. in Acta Veterinaria Hungarica, 67(3), 338-346.
https://doi.org/10.1556/004.2019.035

Blagojević J, Šekler M, Rajičić M, Bajić B, Budinski I, Jovanović V, Adnađević T, Vidanović D, Matović K, Vujošević M. The prevalence of pathogenic forms of Leptospira in natural populations of small wild mammals in Serbia.. in Acta Veterinaria Hungarica. 2019;67(3):338-346.
doi:10.1556/004.2019.035 .

Blagojević, Jelena, Šekler, Milanko, Rajičić, Marija, Bajić, Branka, Budinski, Ivana, Jovanović, Vladimir, Adnađević, Tanja, Vidanović, Dejan, Matović, Kazimir, Vujošević, Mladen, "The prevalence of pathogenic forms of Leptospira in natural populations of small wild mammals in Serbia." in Acta Veterinaria Hungarica, 67, no. 3 (2019):338-346,
https://doi.org/10.1556/004.2019.035 . .

TY  - JOUR
AU  - Budinski, Ivana
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Bajić, Branka
AU  - Adnađević, Tanja
AU  - Paunović, Milan
AU  - Vujošević, Mladen
PY  - 2019
UR  - http://dx.plos.org/10.1371/journal.pone.0210321
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6353099
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3262
AB  - Migratory behaviour, sociality and roost selection have a great impact on the population structure of one species. Many bat species live in groups, and movements between summer and hibernation sites are common in temperate bats. The Mediterranean horseshoe bat Rhinolophus euryale is a cave-dwelling species that exhibits roost philopatry and undertakes seasonal movements which are usually shorter than 50 km. Its distribution in Serbia is restricted to karstic areas in western and eastern parts of the country, with a lack of known roosts between them. In this study, microsatellite markers were used to evaluate genetic variation in this species in the Central Balkans. Specifically, spatial genetic structuring between geographic regions and relatedness within different colony types were assessed. All analysed loci were polymorphic, and there was no significant inbreeding coefficient recorded. A moderate degree of genetic differentiation among the sampled colonies was found, and significant isolation by distance was recorded. Our results revealed that populations show a tendency to segregate into three clusters. Unexpectedly, populations from Montenegro and Eastern Serbia tended to group into one cluster, while populations from Western Serbia and Slovenia represented second and third cluster, respectively. The majority of variance was partitioned within colonies, and only a small but significant portion among clusters. Average relatedness within colony members was close to zero, did not differ significantly between the different colony types, and kinship is unlikely to be a major grouping mechanism in this species.
T2  - PloS One
T1  - Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans.
IS  - 1
VL  - 14
DO  - 10.1371/journal.pone.0210321
SP  - e0210321
ER  - 

@article{
author = "Budinski, Ivana and Blagojević, Jelena and Jovanović, Vladimir and Bajić, Branka and Adnađević, Tanja and Paunović, Milan and Vujošević, Mladen",
year = "2019",
abstract = "Migratory behaviour, sociality and roost selection have a great impact on the population structure of one species. Many bat species live in groups, and movements between summer and hibernation sites are common in temperate bats. The Mediterranean horseshoe bat Rhinolophus euryale is a cave-dwelling species that exhibits roost philopatry and undertakes seasonal movements which are usually shorter than 50 km. Its distribution in Serbia is restricted to karstic areas in western and eastern parts of the country, with a lack of known roosts between them. In this study, microsatellite markers were used to evaluate genetic variation in this species in the Central Balkans. Specifically, spatial genetic structuring between geographic regions and relatedness within different colony types were assessed. All analysed loci were polymorphic, and there was no significant inbreeding coefficient recorded. A moderate degree of genetic differentiation among the sampled colonies was found, and significant isolation by distance was recorded. Our results revealed that populations show a tendency to segregate into three clusters. Unexpectedly, populations from Montenegro and Eastern Serbia tended to group into one cluster, while populations from Western Serbia and Slovenia represented second and third cluster, respectively. The majority of variance was partitioned within colonies, and only a small but significant portion among clusters. Average relatedness within colony members was close to zero, did not differ significantly between the different colony types, and kinship is unlikely to be a major grouping mechanism in this species.",
journal = "PloS One",
title = "Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans.",
number = "1",
volume = "14",
doi = "10.1371/journal.pone.0210321",
pages = "e0210321"
}

Budinski, I., Blagojević, J., Jovanović, V., Bajić, B., Adnađević, T., Paunović, M.,& Vujošević, M.. (2019). Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans.. in PloS One, 14(1), e0210321.
https://doi.org/10.1371/journal.pone.0210321

Budinski I, Blagojević J, Jovanović V, Bajić B, Adnađević T, Paunović M, Vujošević M. Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans.. in PloS One. 2019;14(1):e0210321.
doi:10.1371/journal.pone.0210321 .

Budinski, Ivana, Blagojević, Jelena, Jovanović, Vladimir, Bajić, Branka, Adnađević, Tanja, Paunović, Milan, Vujošević, Mladen, "Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans." in PloS One, 14, no. 1 (2019):e0210321,
https://doi.org/10.1371/journal.pone.0210321 . .

TY  - CONF
AU  - Jojić, Vida
AU  - Čabrilo, Borislav
AU  - Vujošević, Mladen
AU  - Jovanović, Vladimir
AU  - Bjelić-Čabrilo, Olivera
AU  - Blagojević, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5638
AB  - Developmental stability (DS), along with canalization, represents an element of developmental
homeostasis (DH). DH is defined as the mechanism responsible for ensuring phenotypic
constancy in organisms despite the great variability of genetic and environmental features.
DS is usually measured by fluctuating asymmetry (FA) which refers to the minor, random
differences between the two sides in bilaterally symmetric traits. The factors that cause
FA can be either genetic or environmental in origin. In this study we used 276 mandibles
and 323 crania of adult yellow-necked mice (Apodemus flavicollis) featured by the frequent
presence of supernumerary B chromosomes (Bs). We investigated the associations between
developmental stability and susceptibility to nematode parasitism in this species in the
context of Bs presence or absence. In agreement with prevailing view that Bs are genomic
parasites, B carriers would possess lower level of DS, i.e. higher level of FA, compared to noncarriers.
We hypothesized that parasitized individuals should be more asymmetric as well.
By applying landmark-based geometric morphometrics, we estimated the levels of FA (FA10a
indices) for mandibular size and shape and cranial shape in non-parasitized B non-carriers
(NPB0), parasitized B non-carriers (PB0), non-parasitized B carriers (NPB+) and parasitized B
carriers (PB+). According to the hypotheses mentioned above, NPB0 mice would possess the
lowest FA10a indices. Although our results revealed no significant differences in the levels
of FA, NPB0 individuals are characterized by the lowest FA10a index for mandibular size, but
the highest FA10a index for cranial shape. Additionally, the similar levels of FA estimated for
PB0 and PB+ mice indicated that B chromosomes are not linked to developmental stability
and susceptibility to nematode parasitism in this species.
PB  - Belgrade: Serbian Genetic Society
C3  - 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
T1  - Developmental stability, B chromosomes and suspectibility to parasitism in the yellow-necked mouse Apodemus flavicollis
SP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5638
ER  - 

@conference{
author = "Jojić, Vida and Čabrilo, Borislav and Vujošević, Mladen and Jovanović, Vladimir and Bjelić-Čabrilo, Olivera and Blagojević, Jelena",
year = "2019",
abstract = "Developmental stability (DS), along with canalization, represents an element of developmental
homeostasis (DH). DH is defined as the mechanism responsible for ensuring phenotypic
constancy in organisms despite the great variability of genetic and environmental features.
DS is usually measured by fluctuating asymmetry (FA) which refers to the minor, random
differences between the two sides in bilaterally symmetric traits. The factors that cause
FA can be either genetic or environmental in origin. In this study we used 276 mandibles
and 323 crania of adult yellow-necked mice (Apodemus flavicollis) featured by the frequent
presence of supernumerary B chromosomes (Bs). We investigated the associations between
developmental stability and susceptibility to nematode parasitism in this species in the
context of Bs presence or absence. In agreement with prevailing view that Bs are genomic
parasites, B carriers would possess lower level of DS, i.e. higher level of FA, compared to noncarriers.
We hypothesized that parasitized individuals should be more asymmetric as well.
By applying landmark-based geometric morphometrics, we estimated the levels of FA (FA10a
indices) for mandibular size and shape and cranial shape in non-parasitized B non-carriers
(NPB0), parasitized B non-carriers (PB0), non-parasitized B carriers (NPB+) and parasitized B
carriers (PB+). According to the hypotheses mentioned above, NPB0 mice would possess the
lowest FA10a indices. Although our results revealed no significant differences in the levels
of FA, NPB0 individuals are characterized by the lowest FA10a index for mandibular size, but
the highest FA10a index for cranial shape. Additionally, the similar levels of FA estimated for
PB0 and PB+ mice indicated that B chromosomes are not linked to developmental stability
and susceptibility to nematode parasitism in this species.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia",
title = "Developmental stability, B chromosomes and suspectibility to parasitism in the yellow-necked mouse Apodemus flavicollis",
pages = "139",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5638"
}

Jojić, V., Čabrilo, B., Vujošević, M., Jovanović, V., Bjelić-Čabrilo, O.,& Blagojević, J.. (2019). Developmental stability, B chromosomes and suspectibility to parasitism in the yellow-necked mouse Apodemus flavicollis. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society., 139.
https://hdl.handle.net/21.15107/rcub_ibiss_5638

Jojić V, Čabrilo B, Vujošević M, Jovanović V, Bjelić-Čabrilo O, Blagojević J. Developmental stability, B chromosomes and suspectibility to parasitism in the yellow-necked mouse Apodemus flavicollis. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia. 2019;:139.
https://hdl.handle.net/21.15107/rcub_ibiss_5638 .

Jojić, Vida, Čabrilo, Borislav, Vujošević, Mladen, Jovanović, Vladimir, Bjelić-Čabrilo, Olivera, Blagojević, Jelena, "Developmental stability, B chromosomes and suspectibility to parasitism in the yellow-necked mouse Apodemus flavicollis" in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia (2019):139,
https://hdl.handle.net/21.15107/rcub_ibiss_5638 .

TY  - CONF
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Budinski, Ivana
AU  - Rajičić, Marija
AU  - Adnađević, Tanja
AU  - Vujošević, Mladen
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4850
AB  - Water, being of critical importance for the survival of life on the planet, requires our permanent care and attention. There is a growing concern about the genotoxicity of complex environmental mixtures present in surface waters, due to the risk of genetic damage and cancer, both in aquatic organisms and humans. The present study focuses on exploring the status of water pollution in areas with combined industrial and agricultural activities in order to estimate the magnitude of toxicity and genotoxicity using the Allium test. We collected samples from the Sava and Danube rivers, upstream and downstream of Šabac (Sava River) and Smederevo (Danube River). In both rivers sampling was done in periods of low and high water levels. Levels of toxicity were low in both rivers, although the presence of organic pollution was seen from higher root mean length in all sample groups than in negative controls. The highest values of genotoxicity at locations upstream of both Šabac and Smederevo were obtained in samples collected in a period of high water level, but only the upstream sample from the Sava River had reached a significant level of genotoxicity. This can be explained as a consequence of intensive agricultural activities. Our results indicate that communal town and industrial wastewaters influence river water quality more significantly during low level regimes, while high water levels increase the risk of exposure to chemicals used in agriculture.
PB  - Belgrade: Serbian Genetic Society
C3  - Book of Proceedings 6th Congress of Serbian Genetic Society
T1  - Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes
SP  - 7
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4850
ER  - 

@conference{
author = "Blagojević, Jelena and Jovanović, Vladimir and Budinski, Ivana and Rajičić, Marija and Adnađević, Tanja and Vujošević, Mladen",
year = "2019",
abstract = "Water, being of critical importance for the survival of life on the planet, requires our permanent care and attention. There is a growing concern about the genotoxicity of complex environmental mixtures present in surface waters, due to the risk of genetic damage and cancer, both in aquatic organisms and humans. The present study focuses on exploring the status of water pollution in areas with combined industrial and agricultural activities in order to estimate the magnitude of toxicity and genotoxicity using the Allium test. We collected samples from the Sava and Danube rivers, upstream and downstream of Šabac (Sava River) and Smederevo (Danube River). In both rivers sampling was done in periods of low and high water levels. Levels of toxicity were low in both rivers, although the presence of organic pollution was seen from higher root mean length in all sample groups than in negative controls. The highest values of genotoxicity at locations upstream of both Šabac and Smederevo were obtained in samples collected in a period of high water level, but only the upstream sample from the Sava River had reached a significant level of genotoxicity. This can be explained as a consequence of intensive agricultural activities. Our results indicate that communal town and industrial wastewaters influence river water quality more significantly during low level regimes, while high water levels increase the risk of exposure to chemicals used in agriculture.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "Book of Proceedings 6th Congress of Serbian Genetic Society",
title = "Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes",
pages = "7-14",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4850"
}

Blagojević, J., Jovanović, V., Budinski, I., Rajičić, M., Adnađević, T.,& Vujošević, M.. (2019). Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes. in Book of Proceedings 6th Congress of Serbian Genetic Society
Belgrade: Serbian Genetic Society., 7-14.
https://hdl.handle.net/21.15107/rcub_ibiss_4850

Blagojević J, Jovanović V, Budinski I, Rajičić M, Adnađević T, Vujošević M. Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes. in Book of Proceedings 6th Congress of Serbian Genetic Society. 2019;:7-14.
https://hdl.handle.net/21.15107/rcub_ibiss_4850 .

Blagojević, Jelena, Jovanović, Vladimir, Budinski, Ivana, Rajičić, Marija, Adnađević, Tanja, Vujošević, Mladen, "Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes" in Book of Proceedings 6th Congress of Serbian Genetic Society (2019):7-14,
https://hdl.handle.net/21.15107/rcub_ibiss_4850 .

TY  - CONF
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Budinski, Ivana
AU  - Rajičić, Marija
AU  - Adnađević, Tanja
AU  - Vujošević, Mladen
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4846
AB  - Water, being of critical importance for the survival of life on the planet, requires our
permanent care and attention. There is a growing concern about the genotoxicity of complex
environmental mixtures present in surface waters, due to the risk of genetic damage and
cancer, both in aquatic organisms and humans. Allium anaphase-telophase test has been
accepted as a promising tool to detect, among other things, toxicity and genotoxicity of river
water. The present study has focused on exploring the status of water pollution in areas
with combined industrial and agricultural activities in order to estimate the magnitude
of toxicity and genotoxicity using the Allium test. We collected samples of surface water
from the Sava River, upstream and downstream from the town of Šabaca nd at the Danube
River upstream and downstream from the town Smederevo. We analyzed using the Allium
anaphase-telophase test. In both rivers sampling was done in periods of low and high water
level. While at low water level genotoxicity was higher at points downstream of Šabaca nd
Smederevo, samples collected in periods of highwater level were more genotoxic upstream
from the towns. This can be explained as a consequence of agricultural activities.
PB  - Belgrade: Serbian Genetic Society
C3  - 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
T1  - Influence of town pollutions on levels of water genotoxicity during different water level regimes
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4846
ER  - 

@conference{
author = "Blagojević, Jelena and Jovanović, Vladimir and Budinski, Ivana and Rajičić, Marija and Adnađević, Tanja and Vujošević, Mladen",
year = "2019",
abstract = "Water, being of critical importance for the survival of life on the planet, requires our
permanent care and attention. There is a growing concern about the genotoxicity of complex
environmental mixtures present in surface waters, due to the risk of genetic damage and
cancer, both in aquatic organisms and humans. Allium anaphase-telophase test has been
accepted as a promising tool to detect, among other things, toxicity and genotoxicity of river
water. The present study has focused on exploring the status of water pollution in areas
with combined industrial and agricultural activities in order to estimate the magnitude
of toxicity and genotoxicity using the Allium test. We collected samples of surface water
from the Sava River, upstream and downstream from the town of Šabaca nd at the Danube
River upstream and downstream from the town Smederevo. We analyzed using the Allium
anaphase-telophase test. In both rivers sampling was done in periods of low and high water
level. While at low water level genotoxicity was higher at points downstream of Šabaca nd
Smederevo, samples collected in periods of highwater level were more genotoxic upstream
from the towns. This can be explained as a consequence of agricultural activities.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia",
title = "Influence of town pollutions on levels of water genotoxicity during different water level regimes",
pages = "129",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4846"
}

Blagojević, J., Jovanović, V., Budinski, I., Rajičić, M., Adnađević, T.,& Vujošević, M.. (2019). Influence of town pollutions on levels of water genotoxicity during different water level regimes. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society., 129.
https://hdl.handle.net/21.15107/rcub_ibiss_4846

Blagojević J, Jovanović V, Budinski I, Rajičić M, Adnađević T, Vujošević M. Influence of town pollutions on levels of water genotoxicity during different water level regimes. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia. 2019;:129.
https://hdl.handle.net/21.15107/rcub_ibiss_4846 .

Blagojević, Jelena, Jovanović, Vladimir, Budinski, Ivana, Rajičić, Marija, Adnađević, Tanja, Vujošević, Mladen, "Influence of town pollutions on levels of water genotoxicity during different water level regimes" in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia (2019):129,
https://hdl.handle.net/21.15107/rcub_ibiss_4846 .

TY  - JOUR
AU  - Blagojević, Jelena
AU  - Rajičić, Marija
AU  - Jovanović, Vladimir
AU  - Adnađević, Tanja
AU  - Budinski, Ivana
AU  - Bajić, Branka
AU  - Vujošević, Mladen
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800004B
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2364
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3283
AB  - Arvicoline voles are known as a karyotypically extremely polymorphic group in which the genus Microtus leads with the highest rate of karyotype change. A member of this genus, the European pine vole Microtus subterraneus (de Selys-Longchamps, 1836), is widely distributed in Europe and parts of Asia. There are two cytotypes differing in diploid chromosome number, 2n=54 and 52, each showing different chromosomal polymorphisms. At two localities in southeastern Serbia, Mt. Jastrebac and Vlasina, we found the 2n=52 cytotype. Meiotic preparations from males revealed the presence of asynaptic sex chromosomes. Although asynaptic sex chromosomes are frequent in Microtus, this is the first finding for M. subterraneus. From summarized data it appears that two-thirds of the studied species, mainly from Microtus and Terricola subgenera, possess asynaptic sex chromosomes.
T2  - Archives of Biological Sciences
T1  - One more Microtus species with asynaptic sex chromosomes
IS  - 3
VL  - 70
DO  - 10.2298/ABS171113004B
SP  - 443
EP  - 447
ER  - 

@article{
author = "Blagojević, Jelena and Rajičić, Marija and Jovanović, Vladimir and Adnađević, Tanja and Budinski, Ivana and Bajić, Branka and Vujošević, Mladen",
year = "2018",
abstract = "Arvicoline voles are known as a karyotypically extremely polymorphic group in which the genus Microtus leads with the highest rate of karyotype change. A member of this genus, the European pine vole Microtus subterraneus (de Selys-Longchamps, 1836), is widely distributed in Europe and parts of Asia. There are two cytotypes differing in diploid chromosome number, 2n=54 and 52, each showing different chromosomal polymorphisms. At two localities in southeastern Serbia, Mt. Jastrebac and Vlasina, we found the 2n=52 cytotype. Meiotic preparations from males revealed the presence of asynaptic sex chromosomes. Although asynaptic sex chromosomes are frequent in Microtus, this is the first finding for M. subterraneus. From summarized data it appears that two-thirds of the studied species, mainly from Microtus and Terricola subgenera, possess asynaptic sex chromosomes.",
journal = "Archives of Biological Sciences",
title = "One more Microtus species with asynaptic sex chromosomes",
number = "3",
volume = "70",
doi = "10.2298/ABS171113004B",
pages = "443-447"
}

Blagojević, J., Rajičić, M., Jovanović, V., Adnađević, T., Budinski, I., Bajić, B.,& Vujošević, M.. (2018). One more Microtus species with asynaptic sex chromosomes. in Archives of Biological Sciences, 70(3), 443-447.
https://doi.org/10.2298/ABS171113004B

Blagojević J, Rajičić M, Jovanović V, Adnađević T, Budinski I, Bajić B, Vujošević M. One more Microtus species with asynaptic sex chromosomes. in Archives of Biological Sciences. 2018;70(3):443-447.
doi:10.2298/ABS171113004B .

Blagojević, Jelena, Rajičić, Marija, Jovanović, Vladimir, Adnađević, Tanja, Budinski, Ivana, Bajić, Branka, Vujošević, Mladen, "One more Microtus species with asynaptic sex chromosomes" in Archives of Biological Sciences, 70, no. 3 (2018):443-447,
https://doi.org/10.2298/ABS171113004B . .

TY  - JOUR
AU  - Jovanović, Vladimir
AU  - Čabrilo, Borislav
AU  - Budinski, Ivana
AU  - Bjelić-Čabrilo, Olivera
AU  - Adnađević, Tanja
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
PY  - 2018
UR  - https://www.cambridge.org/core/product/identifier/S0022149X18000548/type/journal_article
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3111
AB  - The yellow-necked mouse, Apodemus flavicollis, can be considered as a model for genetic polymorphism produced by the frequent presence of supernumerary or B chromosomes (Bs). Host genetic background is rarely taken into account in studies of parasite sex ratio. The main aim of this study was to investigate the range of infrapopulation sex ratios for nematode parasites of the yellow-necked mouse and to determine which factors most influence variation in parasite sex ratios. Six nematode species found in the collected yellow-necked mice were analysed. We confirmed the predominant pattern of female-biased sex ratios in vertebrate parasite infrapopulations. The presence of B chromosomes in host genomes played an important role in infrapopulations of Heligmosomoides polygyrus, Syphacia stroma and Trichuris muris, as hosts with B chromosomes carried a higher proportion of males. The relative increase of males in infrapopulations could result from a shift in parasite life history strategy, induced by adaptation to the specific host genotypes (Bs present). In a meta-analysis with previously published data, the sex determination system was demonstrated to play a significant role in nematode sex ratio variation, as well as specific life history patterns, such as the place of egg hatching.
T2  - Journal of Helminthology
T1  - Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis).
DO  - 10.1017/S0022149X18000548
ER  - 

@article{
author = "Jovanović, Vladimir and Čabrilo, Borislav and Budinski, Ivana and Bjelić-Čabrilo, Olivera and Adnađević, Tanja and Blagojević, Jelena and Vujošević, Mladen",
year = "2018",
abstract = "The yellow-necked mouse, Apodemus flavicollis, can be considered as a model for genetic polymorphism produced by the frequent presence of supernumerary or B chromosomes (Bs). Host genetic background is rarely taken into account in studies of parasite sex ratio. The main aim of this study was to investigate the range of infrapopulation sex ratios for nematode parasites of the yellow-necked mouse and to determine which factors most influence variation in parasite sex ratios. Six nematode species found in the collected yellow-necked mice were analysed. We confirmed the predominant pattern of female-biased sex ratios in vertebrate parasite infrapopulations. The presence of B chromosomes in host genomes played an important role in infrapopulations of Heligmosomoides polygyrus, Syphacia stroma and Trichuris muris, as hosts with B chromosomes carried a higher proportion of males. The relative increase of males in infrapopulations could result from a shift in parasite life history strategy, induced by adaptation to the specific host genotypes (Bs present). In a meta-analysis with previously published data, the sex determination system was demonstrated to play a significant role in nematode sex ratio variation, as well as specific life history patterns, such as the place of egg hatching.",
journal = "Journal of Helminthology",
title = "Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis).",
doi = "10.1017/S0022149X18000548"
}

Jovanović, V., Čabrilo, B., Budinski, I., Bjelić-Čabrilo, O., Adnađević, T., Blagojević, J.,& Vujošević, M.. (2018). Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis).. in Journal of Helminthology.
https://doi.org/10.1017/S0022149X18000548

Jovanović V, Čabrilo B, Budinski I, Bjelić-Čabrilo O, Adnađević T, Blagojević J, Vujošević M. Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis).. in Journal of Helminthology. 2018;.
doi:10.1017/S0022149X18000548 .

Jovanović, Vladimir, Čabrilo, Borislav, Budinski, Ivana, Bjelić-Čabrilo, Olivera, Adnađević, Tanja, Blagojević, Jelena, Vujošević, Mladen, "Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis)." in Journal of Helminthology (2018),
https://doi.org/10.1017/S0022149X18000548 . .

TY  - JOUR
AU  - Makunin, Alexey I.
AU  - Rajičić, Marija
AU  - Karamysheva, Tatyana V.
AU  - Romanenko, Svetlana A.
AU  - Druzhkova, Anna S.
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
AU  - Rubtsov, Nikolay B.
AU  - Graphodatsky, Alexander S.
AU  - Trifonov, Vladimir A.
PY  - 2018
UR  - http://link.springer.com/10.1007/s00412-018-0662-0
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29380046
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2969
AB  - Supernumerary chromosomes sporadically arise in many eukaryotic species as a result of genomic rearrangements. If present in a substantial part of species population, those are called B chromosomes, or Bs. This is the case for 70 mammalian species, most of which are rodents. In humans, the most common types of extra chromosomes, sSMCs (small supernumerary marker chromosomes), are diagnosed in approximately 1 of 2000 postnatal cases. Due to low frequency in population, human sSMCs are not considered B chromosomes. Genetic content of both B-chromosomes and sSMCs in most cases remains understudied. Here, we apply microdissection of single chromosomes with subsequent low-pass sequencing on Ion Torrent PGM and Illumina MiSeq to identify unique and repetitive DNA sequences present in a single human sSMC and several B chromosomes in mice Apodemus flavicollis and Apodemus peninsulae. The pipeline for sequencing data analysis was made available in Galaxy interface as an addition to previously published command-line version. Human sSMC was attributed to the proximal part of chromosome 15 long arm, and breakpoints leading to its formation were located into satellite DNA arrays. Genetic content of Apodemus B chromosomes was species-specific, and minor alterations were observed in both species. Common features of Bs in these Apodemus species were satellite DNA and ERV enrichment, as well as the presence of the vaccinia-related kinase gene Vrk1. Understanding of the non-essential genome elements content provides important insights into genome evolution in general.
T2  - Chromosoma
T1  - Low-pass single-chromosome sequencing of human small supernumerary marker chromosomes (sSMCs) and Apodemus B chromosomes.
IS  - 3
VL  - 127
DO  - 10.1007/s00412-018-0662-0
SP  - 301
EP  - 311
ER  - 

@article{
author = "Makunin, Alexey I. and Rajičić, Marija and Karamysheva, Tatyana V. and Romanenko, Svetlana A. and Druzhkova, Anna S. and Blagojević, Jelena and Vujošević, Mladen and Rubtsov, Nikolay B. and Graphodatsky, Alexander S. and Trifonov, Vladimir A.",
year = "2018",
abstract = "Supernumerary chromosomes sporadically arise in many eukaryotic species as a result of genomic rearrangements. If present in a substantial part of species population, those are called B chromosomes, or Bs. This is the case for 70 mammalian species, most of which are rodents. In humans, the most common types of extra chromosomes, sSMCs (small supernumerary marker chromosomes), are diagnosed in approximately 1 of 2000 postnatal cases. Due to low frequency in population, human sSMCs are not considered B chromosomes. Genetic content of both B-chromosomes and sSMCs in most cases remains understudied. Here, we apply microdissection of single chromosomes with subsequent low-pass sequencing on Ion Torrent PGM and Illumina MiSeq to identify unique and repetitive DNA sequences present in a single human sSMC and several B chromosomes in mice Apodemus flavicollis and Apodemus peninsulae. The pipeline for sequencing data analysis was made available in Galaxy interface as an addition to previously published command-line version. Human sSMC was attributed to the proximal part of chromosome 15 long arm, and breakpoints leading to its formation were located into satellite DNA arrays. Genetic content of Apodemus B chromosomes was species-specific, and minor alterations were observed in both species. Common features of Bs in these Apodemus species were satellite DNA and ERV enrichment, as well as the presence of the vaccinia-related kinase gene Vrk1. Understanding of the non-essential genome elements content provides important insights into genome evolution in general.",
journal = "Chromosoma",
title = "Low-pass single-chromosome sequencing of human small supernumerary marker chromosomes (sSMCs) and Apodemus B chromosomes.",
number = "3",
volume = "127",
doi = "10.1007/s00412-018-0662-0",
pages = "301-311"
}

Makunin, A. I., Rajičić, M., Karamysheva, T. V., Romanenko, S. A., Druzhkova, A. S., Blagojević, J., Vujošević, M., Rubtsov, N. B., Graphodatsky, A. S.,& Trifonov, V. A.. (2018). Low-pass single-chromosome sequencing of human small supernumerary marker chromosomes (sSMCs) and Apodemus B chromosomes.. in Chromosoma, 127(3), 301-311.
https://doi.org/10.1007/s00412-018-0662-0

Makunin AI, Rajičić M, Karamysheva TV, Romanenko SA, Druzhkova AS, Blagojević J, Vujošević M, Rubtsov NB, Graphodatsky AS, Trifonov VA. Low-pass single-chromosome sequencing of human small supernumerary marker chromosomes (sSMCs) and Apodemus B chromosomes.. in Chromosoma. 2018;127(3):301-311.
doi:10.1007/s00412-018-0662-0 .

Makunin, Alexey I., Rajičić, Marija, Karamysheva, Tatyana V., Romanenko, Svetlana A., Druzhkova, Anna S., Blagojević, Jelena, Vujošević, Mladen, Rubtsov, Nikolay B., Graphodatsky, Alexander S., Trifonov, Vladimir A., "Low-pass single-chromosome sequencing of human small supernumerary marker chromosomes (sSMCs) and Apodemus B chromosomes." in Chromosoma, 127, no. 3 (2018):301-311,
https://doi.org/10.1007/s00412-018-0662-0 . .

TY  - JOUR
AU  - Vujošević, Mladen
AU  - Rajičić, Marija
AU  - Blagojević, Jelena
PY  - 2018
UR  - http://www.mdpi.com/2073-4425/9/10/487
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3172
AB  - The study of B chromosomes (Bs) started more than a century ago, while their presence in mammals dates since 1965. As the past two decades have seen huge progress in application of molecular techniques, we decided to throw a glance on new data on Bs in mammals and to review them. We listed 85 mammals with Bs that make 1.94% of karyotypically studied species. Contrary to general view, a typical B chromosome in mammals appears both as sub- or metacentric that is the same size as small chromosomes of standard complement. Both karyotypically stable and unstable species possess Bs. The presence of Bs in certain species influences the cell division, the degree of recombination, the development, a number of quantitative characteristics, the host-parasite interactions and their behaviour. There is at least some data on molecular structure of Bs recorded in nearly a quarter of species. Nevertheless, a more detailed molecular composition of Bs presently known for six mammalian species, confirms the presence of protein coding genes, and the transcriptional activity for some of them. Therefore, the idea that Bs are inert is outdated, but the role of Bs is yet to be determined. The maintenance of Bs is obviously not the same for all species, so the current models must be adapted while bearing in mind that Bs are not inactive as it was once thought.
T2  - Genes
T1  - B Chromosomes in Populations of Mammals Revisited
IS  - 10
VL  - 9
DO  - 10.3390/genes9100487
SP  - 487
ER  - 

@article{
author = "Vujošević, Mladen and Rajičić, Marija and Blagojević, Jelena",
year = "2018",
abstract = "The study of B chromosomes (Bs) started more than a century ago, while their presence in mammals dates since 1965. As the past two decades have seen huge progress in application of molecular techniques, we decided to throw a glance on new data on Bs in mammals and to review them. We listed 85 mammals with Bs that make 1.94% of karyotypically studied species. Contrary to general view, a typical B chromosome in mammals appears both as sub- or metacentric that is the same size as small chromosomes of standard complement. Both karyotypically stable and unstable species possess Bs. The presence of Bs in certain species influences the cell division, the degree of recombination, the development, a number of quantitative characteristics, the host-parasite interactions and their behaviour. There is at least some data on molecular structure of Bs recorded in nearly a quarter of species. Nevertheless, a more detailed molecular composition of Bs presently known for six mammalian species, confirms the presence of protein coding genes, and the transcriptional activity for some of them. Therefore, the idea that Bs are inert is outdated, but the role of Bs is yet to be determined. The maintenance of Bs is obviously not the same for all species, so the current models must be adapted while bearing in mind that Bs are not inactive as it was once thought.",
journal = "Genes",
title = "B Chromosomes in Populations of Mammals Revisited",
number = "10",
volume = "9",
doi = "10.3390/genes9100487",
pages = "487"
}

Vujošević, M., Rajičić, M.,& Blagojević, J.. (2018). B Chromosomes in Populations of Mammals Revisited. in Genes, 9(10), 487.
https://doi.org/10.3390/genes9100487

Vujošević M, Rajičić M, Blagojević J. B Chromosomes in Populations of Mammals Revisited. in Genes. 2018;9(10):487.
doi:10.3390/genes9100487 .

Vujošević, Mladen, Rajičić, Marija, Blagojević, Jelena, "B Chromosomes in Populations of Mammals Revisited" in Genes, 9, no. 10 (2018):487,
https://doi.org/10.3390/genes9100487 . .

TY  - JOUR
AU  - Čabrilo, Borislav
AU  - Jovanović, Vladimir
AU  - Bjelić Čabrilo, Olivera
AU  - Budinski, Ivana
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
PY  - 2018
UR  - https://content.sciendo.com/view/journals/helm/55/3/article-p247.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3122
AB  - Fifty-one yellow-necked mice from the Obedska bara locality were analysed for the presence of intestinal nematode parasites in order to assert whether there was a host sex bias in infection. Previous research indicated that males would be the more infected sex, either due to the immunosuppressive effect of testosterone or their different allocation of resources towards immune defence. Quantitative infection parameters were compared between host sexes for all nematode species and nematodes in general. In addition, the influence of host sex, age, total body length, body mass and presence of other nematode species on parasite abundance was analysed. No statistically significant differences between males and females were noted for any of the studied quantitative parameters, leading to an absence of sex-biased parasitism in this study.
T2  - Helminthologia
T1  - Is there a host sex bias in intestinal nematode parasitism of the yellow-necked mouse (Apodemus flavicollis) at Obedska bara pond, Serbia?
IS  - 3
VL  - 55
DO  - 10.2478/helm-2018-0018
SP  - 247
EP  - 250
ER  - 

@article{
author = "Čabrilo, Borislav and Jovanović, Vladimir and Bjelić Čabrilo, Olivera and Budinski, Ivana and Blagojević, Jelena and Vujošević, Mladen",
year = "2018",
abstract = "Fifty-one yellow-necked mice from the Obedska bara locality were analysed for the presence of intestinal nematode parasites in order to assert whether there was a host sex bias in infection. Previous research indicated that males would be the more infected sex, either due to the immunosuppressive effect of testosterone or their different allocation of resources towards immune defence. Quantitative infection parameters were compared between host sexes for all nematode species and nematodes in general. In addition, the influence of host sex, age, total body length, body mass and presence of other nematode species on parasite abundance was analysed. No statistically significant differences between males and females were noted for any of the studied quantitative parameters, leading to an absence of sex-biased parasitism in this study.",
journal = "Helminthologia",
title = "Is there a host sex bias in intestinal nematode parasitism of the yellow-necked mouse (Apodemus flavicollis) at Obedska bara pond, Serbia?",
number = "3",
volume = "55",
doi = "10.2478/helm-2018-0018",
pages = "247-250"
}

Čabrilo, B., Jovanović, V., Bjelić Čabrilo, O., Budinski, I., Blagojević, J.,& Vujošević, M.. (2018). Is there a host sex bias in intestinal nematode parasitism of the yellow-necked mouse (Apodemus flavicollis) at Obedska bara pond, Serbia?. in Helminthologia, 55(3), 247-250.
https://doi.org/10.2478/helm-2018-0018

Čabrilo B, Jovanović V, Bjelić Čabrilo O, Budinski I, Blagojević J, Vujošević M. Is there a host sex bias in intestinal nematode parasitism of the yellow-necked mouse (Apodemus flavicollis) at Obedska bara pond, Serbia?. in Helminthologia. 2018;55(3):247-250.
doi:10.2478/helm-2018-0018 .

Čabrilo, Borislav, Jovanović, Vladimir, Bjelić Čabrilo, Olivera, Budinski, Ivana, Blagojević, Jelena, Vujošević, Mladen, "Is there a host sex bias in intestinal nematode parasitism of the yellow-necked mouse (Apodemus flavicollis) at Obedska bara pond, Serbia?" in Helminthologia, 55, no. 3 (2018):247-250,
https://doi.org/10.2478/helm-2018-0018 . .

TY  - CONF
AU  - Budinski, Ivana
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Bajić, Branka
AU  - Rajičić, Marija
AU  - Paunović, Milan
AU  - Vujošević, Mladen
PY  - 2017
UR  - http://ebrs2017.eus/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2944
AB  - Mediterranean horseshoe bat is considered to be mostly sedentary species, with seasonal movements usually shorter than 50 km. Known roosts of Rhinolophus euryale in Serbia are distributed in western and eastern parts of the country, overlapping with karst distribution. All but one roosts recorded in last 50 years were caves. The distance between caves in Western and Eastern Serbia is greater than 150 km, and the environment between them is devoid of caves. Furthermore, there were no recent records of this species from Central Serbia. Bearing in mind the non-migratory nature of this species, our aim was to test for population structuring (isolation by distance pattern) among different geographic regions.
C3  - 14th European Bat Research Symposium
T1  - Population Genetic Structure of Mediterranean Horseshoe Bat Rhinolophus Euryale in Serbia
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2944
ER  - 

@conference{
author = "Budinski, Ivana and Blagojević, Jelena and Jovanović, Vladimir and Bajić, Branka and Rajičić, Marija and Paunović, Milan and Vujošević, Mladen",
year = "2017",
abstract = "Mediterranean horseshoe bat is considered to be mostly sedentary species, with seasonal movements usually shorter than 50 km. Known roosts of Rhinolophus euryale in Serbia are distributed in western and eastern parts of the country, overlapping with karst distribution. All but one roosts recorded in last 50 years were caves. The distance between caves in Western and Eastern Serbia is greater than 150 km, and the environment between them is devoid of caves. Furthermore, there were no recent records of this species from Central Serbia. Bearing in mind the non-migratory nature of this species, our aim was to test for population structuring (isolation by distance pattern) among different geographic regions.",
journal = "14th European Bat Research Symposium",
title = "Population Genetic Structure of Mediterranean Horseshoe Bat Rhinolophus Euryale in Serbia",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2944"
}

Budinski, I., Blagojević, J., Jovanović, V., Bajić, B., Rajičić, M., Paunović, M.,& Vujošević, M.. (2017). Population Genetic Structure of Mediterranean Horseshoe Bat Rhinolophus Euryale in Serbia. in 14th European Bat Research Symposium.
https://hdl.handle.net/21.15107/rcub_ibiss_2944

Budinski I, Blagojević J, Jovanović V, Bajić B, Rajičić M, Paunović M, Vujošević M. Population Genetic Structure of Mediterranean Horseshoe Bat Rhinolophus Euryale in Serbia. in 14th European Bat Research Symposium. 2017;.
https://hdl.handle.net/21.15107/rcub_ibiss_2944 .

Budinski, Ivana, Blagojević, Jelena, Jovanović, Vladimir, Bajić, Branka, Rajičić, Marija, Paunović, Milan, Vujošević, Mladen, "Population Genetic Structure of Mediterranean Horseshoe Bat Rhinolophus Euryale in Serbia" in 14th European Bat Research Symposium (2017),
https://hdl.handle.net/21.15107/rcub_ibiss_2944 .

TY  - JOUR
AU  - Rajičić, Marija
AU  - Romanenko, Svetlana A.
AU  - Karamysheva, Tatyana V.
AU  - Blagojević, Jelena
AU  - Adnađević, Tanja
AU  - Budinski, Ivana
AU  - Bogdanov, Aleksey S.
AU  - Trifonov, Vladimir A.
AU  - Rubtsov, Nikolay B.
AU  - Vujošević, Mladen
PY  - 2017
UR  - http://dx.plos.org/10.1371/journal.pone.0172704
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2624
AB  - B chromosomes (Bs) are known for more than hundred years but their origin, structure and pattern of evolution are not well understood. In the past few years new methodological approaches, involving isolation of Bs followed by whole DNA amplification, DNA probe generation, and fluorescent in situ hybridization (FISH) or the B chromosome DNA sequencing, has allowed detailed analysis of their origin and molecular structure in different species. In this study we explored the origin of Bs in the yellow-necked wood mouse, Apodemus flavicollis, using generation of microdissected DNA probes followed by FISH on metaphase chromosomes. Bs of A. flavicollis were successfully isolated and DNA was used as the template for B-specific probes for the first time. We revealed homology of DNA derived from the analyzed B chromosomes to the pericentromeric region (PR) of sex chromosomes and subtelomeric region of two pairs of small autosomes, but lower homology to the rest of the Y chromosome. Moreover, all analysed Bs had the same structure regardless of their number per individual or the great geographic distance between examined populations from the Balkan Peninsula (Serbia) and Eastern Europe (south region of Russia and central Belarus). Therefore, it was suggested that B chromosomes in A. flavicollis have a unique common origin from the PR of sex chromosomes, and/or similar evolutionary pattern.
T2  - PloS one
T1  - The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)—Break rules but keep playing the game
IS  - 3
VL  - 12
DO  - 10.1371/journal.pone.0172704
SP  - e0172704
EP  - e0172704
ER  - 

@article{
editor = "Houben, Andreas",
author = "Rajičić, Marija and Romanenko, Svetlana A. and Karamysheva, Tatyana V. and Blagojević, Jelena and Adnađević, Tanja and Budinski, Ivana and Bogdanov, Aleksey S. and Trifonov, Vladimir A. and Rubtsov, Nikolay B. and Vujošević, Mladen",
year = "2017",
abstract = "B chromosomes (Bs) are known for more than hundred years but their origin, structure and pattern of evolution are not well understood. In the past few years new methodological approaches, involving isolation of Bs followed by whole DNA amplification, DNA probe generation, and fluorescent in situ hybridization (FISH) or the B chromosome DNA sequencing, has allowed detailed analysis of their origin and molecular structure in different species. In this study we explored the origin of Bs in the yellow-necked wood mouse, Apodemus flavicollis, using generation of microdissected DNA probes followed by FISH on metaphase chromosomes. Bs of A. flavicollis were successfully isolated and DNA was used as the template for B-specific probes for the first time. We revealed homology of DNA derived from the analyzed B chromosomes to the pericentromeric region (PR) of sex chromosomes and subtelomeric region of two pairs of small autosomes, but lower homology to the rest of the Y chromosome. Moreover, all analysed Bs had the same structure regardless of their number per individual or the great geographic distance between examined populations from the Balkan Peninsula (Serbia) and Eastern Europe (south region of Russia and central Belarus). Therefore, it was suggested that B chromosomes in A. flavicollis have a unique common origin from the PR of sex chromosomes, and/or similar evolutionary pattern.",
journal = "PloS one",
title = "The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)—Break rules but keep playing the game",
number = "3",
volume = "12",
doi = "10.1371/journal.pone.0172704",
pages = "e0172704-e0172704"
}

Houben, A., Rajičić, M., Romanenko, S. A., Karamysheva, T. V., Blagojević, J., Adnađević, T., Budinski, I., Bogdanov, A. S., Trifonov, V. A., Rubtsov, N. B.,& Vujošević, M.. (2017). The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)—Break rules but keep playing the game. in PloS one, 12(3), e0172704-e0172704.
https://doi.org/10.1371/journal.pone.0172704

Houben A, Rajičić M, Romanenko SA, Karamysheva TV, Blagojević J, Adnađević T, Budinski I, Bogdanov AS, Trifonov VA, Rubtsov NB, Vujošević M. The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)—Break rules but keep playing the game. in PloS one. 2017;12(3):e0172704-e0172704.
doi:10.1371/journal.pone.0172704 .

Houben, Andreas, Rajičić, Marija, Romanenko, Svetlana A., Karamysheva, Tatyana V., Blagojević, Jelena, Adnađević, Tanja, Budinski, Ivana, Bogdanov, Aleksey S., Trifonov, Vladimir A., Rubtsov, Nikolay B., Vujošević, Mladen, "The origin of B chromosomes in yellow-necked mice (Apodemus flavicollis)—Break rules but keep playing the game" in PloS one, 12, no. 3 (2017):e0172704-e0172704,
https://doi.org/10.1371/journal.pone.0172704 . .