TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Milenković, Dragan
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4144
AB  - In a series of our previous works, we revealed the beneficial effects of applied soy isoflavones (genistein or
daidzein) on the wide context of corticosteroidogenesis in vivo, in a rat model of the andropause. Soy isoflavones
decreased the circulating levels of pituitary adrenocorticotropic hormone, inhibited aldosterone secretion, as
well as corticosterone production and secretion, but stimulated dehydroepiandrosterone secretion, all in
andropausal rats. In vitro studies indicate that the mechanism underlying these hormonal changes relies on inhibition of the pituitary tyrosine kinase and adrenocortical 3β-hydroxysteroid dehydrogenase enzymes by soy
isoflavones. Although the clinical studies are in their infancy, the opinion is that genistein and daidzein have
therapeutic potential for the safe treatment of ageing-caused androgen deprivation and glucocorticoid excess
with related metabolic/hemodynamic issues in males. Our accumulated experience and knowledge in the field of
biomedical effects of plant polyphenols have provided a platform for potential recommending the agenda to
organize and accelerate experimental research aimed at producing the optimal supplementation. We hypothesize
that an in vivo approach should first be exploited in the sequence of investigative steps, followed by in vitro
studies and synchronously conducted molecular docking analyses. In vivo research, besides establishing the
margin of exposure safety or adjustment of the correct polyphenol dose, enables identification and quantification
of the metabolites of applied polyphenols in the blood. Subsequent in vitro exploitation of the metabolites and
related docking analyses provide clarification of the molecular mechanisms of action of applied polyphenols.
Chemical modification of the polyphenol structure or coupling it with nanoparticles might be the next step in
optimizing the design of supplementation. Selected, intact or chemically-modified polyphenol molecules should
be included in preclinical studies on a more closely-related species, while clinical studies would finally assess the
safety and effectiveness of a polyphenol-based remedial strategy. The final supplement represents a product of an
appropriate technological process, conducted in accordance with the recommendations derived from the preceding research.
PB  - Elsevir
T2  - Medical Hypotheses
T1  - Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design
VL  - 148
DO  - 10.1016/j.mehy.2021.110516
SP  - 110516
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka  and Filipović, Branko and Milenković, Dragan and Jakovljević, Vladimir and Milošević, Verica",
year = "2021",
abstract = "In a series of our previous works, we revealed the beneficial effects of applied soy isoflavones (genistein or
daidzein) on the wide context of corticosteroidogenesis in vivo, in a rat model of the andropause. Soy isoflavones
decreased the circulating levels of pituitary adrenocorticotropic hormone, inhibited aldosterone secretion, as
well as corticosterone production and secretion, but stimulated dehydroepiandrosterone secretion, all in
andropausal rats. In vitro studies indicate that the mechanism underlying these hormonal changes relies on inhibition of the pituitary tyrosine kinase and adrenocortical 3β-hydroxysteroid dehydrogenase enzymes by soy
isoflavones. Although the clinical studies are in their infancy, the opinion is that genistein and daidzein have
therapeutic potential for the safe treatment of ageing-caused androgen deprivation and glucocorticoid excess
with related metabolic/hemodynamic issues in males. Our accumulated experience and knowledge in the field of
biomedical effects of plant polyphenols have provided a platform for potential recommending the agenda to
organize and accelerate experimental research aimed at producing the optimal supplementation. We hypothesize
that an in vivo approach should first be exploited in the sequence of investigative steps, followed by in vitro
studies and synchronously conducted molecular docking analyses. In vivo research, besides establishing the
margin of exposure safety or adjustment of the correct polyphenol dose, enables identification and quantification
of the metabolites of applied polyphenols in the blood. Subsequent in vitro exploitation of the metabolites and
related docking analyses provide clarification of the molecular mechanisms of action of applied polyphenols.
Chemical modification of the polyphenol structure or coupling it with nanoparticles might be the next step in
optimizing the design of supplementation. Selected, intact or chemically-modified polyphenol molecules should
be included in preclinical studies on a more closely-related species, while clinical studies would finally assess the
safety and effectiveness of a polyphenol-based remedial strategy. The final supplement represents a product of an
appropriate technological process, conducted in accordance with the recommendations derived from the preceding research.",
publisher = "Elsevir",
journal = "Medical Hypotheses",
title = "Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design",
volume = "148",
doi = "10.1016/j.mehy.2021.110516",
pages = "110516"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B., Filipović, B., Milenković, D., Jakovljević, V.,& Milošević, V.. (2021). Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design. in Medical Hypotheses
Elsevir., 148, 110516.
https://doi.org/10.1016/j.mehy.2021.110516

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B, Milenković D, Jakovljević V, Milošević V. Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design. in Medical Hypotheses. 2021;148:110516.
doi:10.1016/j.mehy.2021.110516 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka , Filipović, Branko, Milenković, Dragan, Jakovljević, Vladimir, Milošević, Verica, "Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design" in Medical Hypotheses, 148 (2021):110516,
https://doi.org/10.1016/j.mehy.2021.110516 . .

TY  - JOUR
AU  - Sretenović, Jasmina
AU  - Joksimović Jović, Jovana
AU  - Srejović, Ivan
AU  - Živković, Vladimir
AU  - Mihajlović, Katarina
AU  - Labudović-Borović, Milica
AU  - Trifunović, Svetlana
AU  - Milošević, Verica
AU  - Lazić, Dejan
AU  - Bolevich, Sergey
AU  - Jakovljević, Vladimir
AU  - Milosavljević, Zoran
PY  - 2021
UR  - https://bacandrology.biomedcentral.com/articles/10.1186/s12610-021-00134-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4444
AB  - BACKGROUND During the last decades, the abuse of anabolic androgenic steroids (AASs) has become popular among professional and recreational athletes. The abuse of AASs leads to decreased levels of sex hormones, but the available literature a gives very small pool of data regarding the effects of swimming alone or combined with AASs on testicle tissue. The aim of this study was to investigate the effects of four-week administration of nandrolone decanoate and swimming training alone or in combination on morphometric parameters, androgen receptor (AR) and redox state in testicle tissue. The study included Wistar albino male rats, 10 weeks old, classified into 4 groups: control (T-N-), nandrolone (T-N+), swimming training (T+N-) and swimming training with nandrolone (T+N+). The rats from nandrolone (N+) groups received nandrolone decanoate 20 mg/kg b.w.once per week. The rats from training (T+) groups, swam 1 h/day 5 days/week. The isolated testicles were measured, left testicles were routinely processed for histological analysis, while right testicles were homogenized and prepared for the analysis of the following oxidative stress biomarkers: index of lipid peroxidation (TBARS), nitrites, catalase, superoxide dismutase (SOD), and reduced glutathione (GSH). RESULTS Diameter, as well as cross-section area of seminiferous tubules were decreased by 10 % and 21 % (respectively) in the T-N+ group and by 15% and 41 % (respectively) in the T+N+ group compared to control. Interstitium of the testicles was decreased in all experimental groups. Reduction of immunoreactivity of AR in T-N+ group was 22 %, in T+N+ group was 9 % compared to control. TBARS levels were increased in T+N- and T+N+ groups. Nitrites were decreased in T+N+ group. Catalase activity was increased in all experimental groups. Swimming alone or combined with nandrolone decreased the level of GSH compared to control. SOD activity was decreased in T-N+ and T+N+ groups compared to control. CONCLUSIONS Nandrolone alone or combined with swimming decreased morphometric parameters and amount of AR in testicle tissue. Changes in the redox state indicate reproductive dysfunction. RéSUMé: CONTEXTE: Au cours des dernières décennies, l’abus de stéroïdes androgéniques anabolisants (SAA) est devenu populaire parmi les athlètes professionnels et récréatifs. L’abus des SAA conduit à une diminution des niveaux d’hormones sexuelles, mais la littérature sur les effets de la natation seule ou combinée avec des SAA sur les tissus testiculaires est encore très limité. Le but de cette étude était d’étudier les effets de l’administration de quatre semaines de décanoate de nandrolone et de l’entraînement à la natation seuls ou en combinaison sur les paramètres morphométriques, le récepteur aux androgènes (RA) et l’état redox dans le tissu testiculaire. L’étude a inclus des rats mâles Wistar albinos, âgés de 10 semaines, classés en 4 groupes: contrôle (T-N-), nandrolone (T-N+), entraînement à la natation (T+N-) et entraînement à la natation avec nandrolone (T+N+). Les rats des groupes nandrolone (N+) ont reçu du décanoate de nandrolone 20 mg/kg p.c. une fois par semaine. Les rats des groupes entraînement (T+) nageaient 1 h/jour 5 jours/semaine. Les testicules isolés ont été mesurés, les testicules gauches ont été systématiquement traités pour l’analyse histologique tandis que les testicules droits ont été homogénéisés et préparés pour l’analyse des biomarqueurs de stress oxydatif suivants: indice de peroxydation lipidique (TBARS), nitrites, catalase, superoxyde dismutase (SOD) et glutathion réduit (GSH). RéSULTATS: Le diamètre, ainsi que la section transversale des tubules séminifères ont été réduits de 10 % et 21 % (respectivement) dans le groupe T-N+ et de 15 % et 41 % (respectivement) dans le groupe T+N+ par rapport au groupe témoin. L’interstitium des testicules était diminué dans tous les groupes expérimentaux. La réduction de l’immunoréactivité de RA dans le groupe T-N+ était de 22 %, dans le groupe T+N+ était de 9 % par rapport au groupe témoin. Les niveaux de TBARS ont augmenté dans les groupes T+N- et T+N+. Les nitrites ont diminué dans le groupe T+N+. L’activité de la catalase a été augmentée dans tous les groupes expérimentaux. La natation seule ou combinée à la nandrolone a réduit le niveau de GSH par rapport au contrôle. L’activité de la SOD était diminuée dans les groupes T-N+ et T+N+ par rapport au contrôle. CONCLUSIONS: La nandrolone seule ou combinée à la natation a diminué les paramètres morphométriques et la quantité de RA dans le tissu testiculaire. Les changements de l’état redox indiquent un dysfonctionnement de la reproduction.
PB  - London: BioMed Central Ltd
T2  - Basic and Clinical Andrology
T1  - Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats
IS  - 1
VL  - 31
DO  - 10.1186/s12610-021-00134-8
SP  - 17
ER  - 

@article{
author = "Sretenović, Jasmina and Joksimović Jović, Jovana and Srejović, Ivan and Živković, Vladimir and Mihajlović, Katarina and Labudović-Borović, Milica and Trifunović, Svetlana and Milošević, Verica and Lazić, Dejan and Bolevich, Sergey and Jakovljević, Vladimir and Milosavljević, Zoran",
year = "2021",
abstract = "BACKGROUND During the last decades, the abuse of anabolic androgenic steroids (AASs) has become popular among professional and recreational athletes. The abuse of AASs leads to decreased levels of sex hormones, but the available literature a gives very small pool of data regarding the effects of swimming alone or combined with AASs on testicle tissue. The aim of this study was to investigate the effects of four-week administration of nandrolone decanoate and swimming training alone or in combination on morphometric parameters, androgen receptor (AR) and redox state in testicle tissue. The study included Wistar albino male rats, 10 weeks old, classified into 4 groups: control (T-N-), nandrolone (T-N+), swimming training (T+N-) and swimming training with nandrolone (T+N+). The rats from nandrolone (N+) groups received nandrolone decanoate 20 mg/kg b.w.once per week. The rats from training (T+) groups, swam 1 h/day 5 days/week. The isolated testicles were measured, left testicles were routinely processed for histological analysis, while right testicles were homogenized and prepared for the analysis of the following oxidative stress biomarkers: index of lipid peroxidation (TBARS), nitrites, catalase, superoxide dismutase (SOD), and reduced glutathione (GSH). RESULTS Diameter, as well as cross-section area of seminiferous tubules were decreased by 10 % and 21 % (respectively) in the T-N+ group and by 15% and 41 % (respectively) in the T+N+ group compared to control. Interstitium of the testicles was decreased in all experimental groups. Reduction of immunoreactivity of AR in T-N+ group was 22 %, in T+N+ group was 9 % compared to control. TBARS levels were increased in T+N- and T+N+ groups. Nitrites were decreased in T+N+ group. Catalase activity was increased in all experimental groups. Swimming alone or combined with nandrolone decreased the level of GSH compared to control. SOD activity was decreased in T-N+ and T+N+ groups compared to control. CONCLUSIONS Nandrolone alone or combined with swimming decreased morphometric parameters and amount of AR in testicle tissue. Changes in the redox state indicate reproductive dysfunction. RéSUMé: CONTEXTE: Au cours des dernières décennies, l’abus de stéroïdes androgéniques anabolisants (SAA) est devenu populaire parmi les athlètes professionnels et récréatifs. L’abus des SAA conduit à une diminution des niveaux d’hormones sexuelles, mais la littérature sur les effets de la natation seule ou combinée avec des SAA sur les tissus testiculaires est encore très limité. Le but de cette étude était d’étudier les effets de l’administration de quatre semaines de décanoate de nandrolone et de l’entraînement à la natation seuls ou en combinaison sur les paramètres morphométriques, le récepteur aux androgènes (RA) et l’état redox dans le tissu testiculaire. L’étude a inclus des rats mâles Wistar albinos, âgés de 10 semaines, classés en 4 groupes: contrôle (T-N-), nandrolone (T-N+), entraînement à la natation (T+N-) et entraînement à la natation avec nandrolone (T+N+). Les rats des groupes nandrolone (N+) ont reçu du décanoate de nandrolone 20 mg/kg p.c. une fois par semaine. Les rats des groupes entraînement (T+) nageaient 1 h/jour 5 jours/semaine. Les testicules isolés ont été mesurés, les testicules gauches ont été systématiquement traités pour l’analyse histologique tandis que les testicules droits ont été homogénéisés et préparés pour l’analyse des biomarqueurs de stress oxydatif suivants: indice de peroxydation lipidique (TBARS), nitrites, catalase, superoxyde dismutase (SOD) et glutathion réduit (GSH). RéSULTATS: Le diamètre, ainsi que la section transversale des tubules séminifères ont été réduits de 10 % et 21 % (respectivement) dans le groupe T-N+ et de 15 % et 41 % (respectivement) dans le groupe T+N+ par rapport au groupe témoin. L’interstitium des testicules était diminué dans tous les groupes expérimentaux. La réduction de l’immunoréactivité de RA dans le groupe T-N+ était de 22 %, dans le groupe T+N+ était de 9 % par rapport au groupe témoin. Les niveaux de TBARS ont augmenté dans les groupes T+N- et T+N+. Les nitrites ont diminué dans le groupe T+N+. L’activité de la catalase a été augmentée dans tous les groupes expérimentaux. La natation seule ou combinée à la nandrolone a réduit le niveau de GSH par rapport au contrôle. L’activité de la SOD était diminuée dans les groupes T-N+ et T+N+ par rapport au contrôle. CONCLUSIONS: La nandrolone seule ou combinée à la natation a diminué les paramètres morphométriques et la quantité de RA dans le tissu testiculaire. Les changements de l’état redox indiquent un dysfonctionnement de la reproduction.",
publisher = "London: BioMed Central Ltd",
journal = "Basic and Clinical Andrology",
title = "Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats",
number = "1",
volume = "31",
doi = "10.1186/s12610-021-00134-8",
pages = "17"
}

Sretenović, J., Joksimović Jović, J., Srejović, I., Živković, V., Mihajlović, K., Labudović-Borović, M., Trifunović, S., Milošević, V., Lazić, D., Bolevich, S., Jakovljević, V.,& Milosavljević, Z.. (2021). Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats. in Basic and Clinical Andrology
London: BioMed Central Ltd., 31(1), 17.
https://doi.org/10.1186/s12610-021-00134-8

Sretenović J, Joksimović Jović J, Srejović I, Živković V, Mihajlović K, Labudović-Borović M, Trifunović S, Milošević V, Lazić D, Bolevich S, Jakovljević V, Milosavljević Z. Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats. in Basic and Clinical Andrology. 2021;31(1):17.
doi:10.1186/s12610-021-00134-8 .

Sretenović, Jasmina, Joksimović Jović, Jovana, Srejović, Ivan, Živković, Vladimir, Mihajlović, Katarina, Labudović-Borović, Milica, Trifunović, Svetlana, Milošević, Verica, Lazić, Dejan, Bolevich, Sergey, Jakovljević, Vladimir, Milosavljević, Zoran, "Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats" in Basic and Clinical Andrology, 31, no. 1 (2021):17,
https://doi.org/10.1186/s12610-021-00134-8 . .

TY  - JOUR
AU  - Jovičić, Nemanja
AU  - Petrović, Ivica
AU  - Pejnović, Nada
AU  - Ljujić, Biljana
AU  - Miletić Kovačević, Marina
AU  - Pavlović, Slađana
AU  - Jeftić, Ilija
AU  - Đukić, Aleksandar
AU  - Srejović, Ivan
AU  - Jakovljević, Vladimir
AU  - Lukić, Miodrag L.
PY  - 2021
UR  - https://www.frontiersin.org/articles/10.3389/fphar.2021.714683/full
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4697
AB  - Galectin-3 (Gal-3) has diverse roles in inflammatory and autoimmune diseases. There is evidence that Gal-3 plays a role in both type 1 and type 2 diabetes. While the role of Gal-3 expression in immune cells invading the pancreatic islets in the experimental model of type 1 diabetes mellitus has been already studied, the importance of the overexpression of Gal-3 in the target β cells is not defined. Therefore, we used multiple low doses of streptozotocin (MLD-STZ)-induced diabetes in C57Bl/6 mice to analyze the effect of transgenic (TG) overexpression of Gal-3 in β cells. Our results demonstrated that the overexpression of Gal-3 protected β cells from apoptosis and attenuated MLD-STZ-induced hyperglycemia, glycosuria, and ketonuria. The cellular analysis of pancreata and draining lymph nodes showed that Gal-3 overexpression significantly decreased the number of pro-inflammatory cells without affecting the presence of T-regulatory cells. As the application of exogenous interleukin 33 (IL-33) given from the beginning of MLD-STZ diabetes induction attenuates the development of disease, by increasing the presence of regulatory FoxP3+ ST2+ cells, we evaluated the potential synergistic effect of the exogenous IL-33 and TG overexpression of Gal-3 in β cells at the later stage of diabetogenesis. The addition of IL-33 potentiated the survival of β cells and attenuated diabetes even when administered later, after the onset of hyperglycemia (12-18 days), suggesting that protection from apoptosis and immunoregulation by IL-33 may attenuate type 1 diabetes.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Pharmacology
T1  - Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33.
VL  - 12
DO  - 10.3389/fphar.2021.714683
SP  - 714683
ER  - 

@article{
author = "Jovičić, Nemanja and Petrović, Ivica and Pejnović, Nada and Ljujić, Biljana and Miletić Kovačević, Marina and Pavlović, Slađana and Jeftić, Ilija and Đukić, Aleksandar and Srejović, Ivan and Jakovljević, Vladimir and Lukić, Miodrag L.",
year = "2021",
abstract = "Galectin-3 (Gal-3) has diverse roles in inflammatory and autoimmune diseases. There is evidence that Gal-3 plays a role in both type 1 and type 2 diabetes. While the role of Gal-3 expression in immune cells invading the pancreatic islets in the experimental model of type 1 diabetes mellitus has been already studied, the importance of the overexpression of Gal-3 in the target β cells is not defined. Therefore, we used multiple low doses of streptozotocin (MLD-STZ)-induced diabetes in C57Bl/6 mice to analyze the effect of transgenic (TG) overexpression of Gal-3 in β cells. Our results demonstrated that the overexpression of Gal-3 protected β cells from apoptosis and attenuated MLD-STZ-induced hyperglycemia, glycosuria, and ketonuria. The cellular analysis of pancreata and draining lymph nodes showed that Gal-3 overexpression significantly decreased the number of pro-inflammatory cells without affecting the presence of T-regulatory cells. As the application of exogenous interleukin 33 (IL-33) given from the beginning of MLD-STZ diabetes induction attenuates the development of disease, by increasing the presence of regulatory FoxP3+ ST2+ cells, we evaluated the potential synergistic effect of the exogenous IL-33 and TG overexpression of Gal-3 in β cells at the later stage of diabetogenesis. The addition of IL-33 potentiated the survival of β cells and attenuated diabetes even when administered later, after the onset of hyperglycemia (12-18 days), suggesting that protection from apoptosis and immunoregulation by IL-33 may attenuate type 1 diabetes.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Pharmacology",
title = "Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33.",
volume = "12",
doi = "10.3389/fphar.2021.714683",
pages = "714683"
}

Jovičić, N., Petrović, I., Pejnović, N., Ljujić, B., Miletić Kovačević, M., Pavlović, S., Jeftić, I., Đukić, A., Srejović, I., Jakovljević, V.,& Lukić, M. L.. (2021). Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33.. in Frontiers in Pharmacology
Lausanne: Frontiers Media S.A.., 12, 714683.
https://doi.org/10.3389/fphar.2021.714683

Jovičić N, Petrović I, Pejnović N, Ljujić B, Miletić Kovačević M, Pavlović S, Jeftić I, Đukić A, Srejović I, Jakovljević V, Lukić ML. Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33.. in Frontiers in Pharmacology. 2021;12:714683.
doi:10.3389/fphar.2021.714683 .

Jovičić, Nemanja, Petrović, Ivica, Pejnović, Nada, Ljujić, Biljana, Miletić Kovačević, Marina, Pavlović, Slađana, Jeftić, Ilija, Đukić, Aleksandar, Srejović, Ivan, Jakovljević, Vladimir, Lukić, Miodrag L., "Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33." in Frontiers in Pharmacology, 12 (2021):714683,
https://doi.org/10.3389/fphar.2021.714683 . .

TY  - JOUR
AU  - Joksimović Jović, Jovana
AU  - Jović, Nikola
AU  - Sretenović, Jasmina
AU  - Živković, Vladimir
AU  - Nikolić, Maja
AU  - Rudić, Jovan
AU  - Milošević, Verica
AU  - Ristić, Nataša
AU  - Andrić, Kristina
AU  - Dimkić Tomić, Tijana
AU  - Miličić, Biljana
AU  - Jakovljević, Vladimir
PY  - 2021
UR  - https://rep.bioscientifica.com/view/journals/rep/163/1/REP-21-0219.xml
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4771
AB  - Numerous evidence implies complex interrelations between polycystic ovary syndrome (PCOS) and hypertension (HT) in reproductive-age women. In this study, we aimed to investigate the potential strain differences in ovarian morphology, hemodynamic, and biochemical characteristics in an androgen-induced PCOS rat model. A total of 24 rats of 3 weeks old (12 Wistar Kyoto - WK and 12 spontaneously hypertensive rats - SHR) were divided into four groups: WK, WK PCOS, SHR, and SHR PCOS. PCOS was induced by daily s.c. injections of testosterone enanthate (1 mg/100 g body weight) administered for 5 weeks. PCOS induction led to estrus cyclicity cessation, cystic ovarian appearance, and sex hormones disturbances in both strains. The morphometric parameters in ovaries were altered in a manner of PCOS-related changes in both strains (higher number in preantral, atretic, and cystic follicles). Ultrasonographically, a significant decrease in ovarian volume (OV) was registered in PCOS groups but also in SHR compared to WK rats. All blood pressure parameters were higher in SHR compared to WK. PCOS modeling increased systolic, mean arterial, and pulse pressure in WK strain, while in SHR, only mean arterial and pulse pressure were higher. Alterations in oxidative stress parameters could provide a molecular basis for PCOS-related changes: in PCOS groups, thiobarbituric acid reactive substance and superoxide anion radical levels were higher in both strains, while superoxide dismutase and glutathione were significantly lowered.
PB  - Society For Reproduction And Fertility
T2  - Reproduction (Cambridge, England)
T1  - Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.
IS  - 1
VL  - 163
DO  - 10.1530/REP-21-0219
SP  - 11
EP  - 21
ER  - 

@article{
author = "Joksimović Jović, Jovana and Jović, Nikola and Sretenović, Jasmina and Živković, Vladimir and Nikolić, Maja and Rudić, Jovan and Milošević, Verica and Ristić, Nataša and Andrić, Kristina and Dimkić Tomić, Tijana and Miličić, Biljana and Jakovljević, Vladimir",
year = "2021",
abstract = "Numerous evidence implies complex interrelations between polycystic ovary syndrome (PCOS) and hypertension (HT) in reproductive-age women. In this study, we aimed to investigate the potential strain differences in ovarian morphology, hemodynamic, and biochemical characteristics in an androgen-induced PCOS rat model. A total of 24 rats of 3 weeks old (12 Wistar Kyoto - WK and 12 spontaneously hypertensive rats - SHR) were divided into four groups: WK, WK PCOS, SHR, and SHR PCOS. PCOS was induced by daily s.c. injections of testosterone enanthate (1 mg/100 g body weight) administered for 5 weeks. PCOS induction led to estrus cyclicity cessation, cystic ovarian appearance, and sex hormones disturbances in both strains. The morphometric parameters in ovaries were altered in a manner of PCOS-related changes in both strains (higher number in preantral, atretic, and cystic follicles). Ultrasonographically, a significant decrease in ovarian volume (OV) was registered in PCOS groups but also in SHR compared to WK rats. All blood pressure parameters were higher in SHR compared to WK. PCOS modeling increased systolic, mean arterial, and pulse pressure in WK strain, while in SHR, only mean arterial and pulse pressure were higher. Alterations in oxidative stress parameters could provide a molecular basis for PCOS-related changes: in PCOS groups, thiobarbituric acid reactive substance and superoxide anion radical levels were higher in both strains, while superoxide dismutase and glutathione were significantly lowered.",
publisher = "Society For Reproduction And Fertility",
journal = "Reproduction (Cambridge, England)",
title = "Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.",
number = "1",
volume = "163",
doi = "10.1530/REP-21-0219",
pages = "11-21"
}

Joksimović Jović, J., Jović, N., Sretenović, J., Živković, V., Nikolić, M., Rudić, J., Milošević, V., Ristić, N., Andrić, K., Dimkić Tomić, T., Miličić, B.,& Jakovljević, V.. (2021). Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.. in Reproduction (Cambridge, England)
Society For Reproduction And Fertility., 163(1), 11-21.
https://doi.org/10.1530/REP-21-0219

Joksimović Jović J, Jović N, Sretenović J, Živković V, Nikolić M, Rudić J, Milošević V, Ristić N, Andrić K, Dimkić Tomić T, Miličić B, Jakovljević V. Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.. in Reproduction (Cambridge, England). 2021;163(1):11-21.
doi:10.1530/REP-21-0219 .

Joksimović Jović, Jovana, Jović, Nikola, Sretenović, Jasmina, Živković, Vladimir, Nikolić, Maja, Rudić, Jovan, Milošević, Verica, Ristić, Nataša, Andrić, Kristina, Dimkić Tomić, Tijana, Miličić, Biljana, Jakovljević, Vladimir, "Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress." in Reproduction (Cambridge, England), 163, no. 1 (2021):11-21,
https://doi.org/10.1530/REP-21-0219 . .

TY  - CONF
AU  - Jovičić, Nemanja
AU  - Petrović, Ivica
AU  - Pejnović, Nada
AU  - Ljujić, Biljana
AU  - Miletić Kovačević, Marina
AU  - Pavlović, Slađana
AU  - Jeftić, Ilija
AU  - Đukić, Aleksandar
AU  - Srejović, Ivan
AU  - Selaković, Dragica
AU  - Jakovljević, Vladimir
AU  - Lukić, Miodrag L.
PY  - 2021
UR  - https://onlinelibrary.wiley.com/toc/15214141/2021/51/S1
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4880
AB  - Galectin 3 (gal 3) has diverse roles in inflammatory and autoimmune diseases. There is evidence that galectin 3 plays a role in both, type 1 and type 2 diabetes. While the role of Gal‐3 expression in immune cells in experimental type 1 diabetes has been already studied, the importance of the overexpression of Gal‐3 in the target β cells is not defined. We used 10‐12 weeks old C57/BL6 male mice (WT) and C57/BL6 mice with transgenically enhanced Gal‐3 expression in pancreatic β cells (TG). Both groups, received STZ for 5 consecutive days at a dose of 40 mg/kg ip. Mice received exogenous mouse IL‐33 (0.4 μg/injection) i.p., 12th, 14 th, 16 th, and 18 th day after the disease induction. Control animals were treated with intraperitoneally PBS + citrate buffer or IL‐33 + citrate buffer. The overexpression of Gal‐3 protected β cells from apoptosis and attenuated MLD‐STZ induced hyperglycemia, glycosuria and ketonuria. The cellular analysis of pancreata and draining lymph nodes showed that Gal‐ 3 overexpression significantly decreased the number of proinflammatory cells without affecting T regulatory cells. The application of exogenous IL‐33, attenuates the development of disease, by increasing the presence of regulatory FoxP3+ ST2+ cells, and competely abrogate diabetogenesis. We demonstrated the potential synergistic effect of exogenous IL‐33 and TG overexpression of Gal‐3 in β cells Not only enhanced expresion of Gal‐3 in β cells reduced T cell mediated autoimmune inflammatory disease, but also exogenous IL‐33 application had powerful terapeutic effect in TG mice.
PB  - Wiley‐VCH GmbH
C3  - 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
T1  - Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33.
DO  - 10.1002/eji.202170200
SP  - 378
ER  - 

@conference{
author = "Jovičić, Nemanja and Petrović, Ivica and Pejnović, Nada and Ljujić, Biljana and Miletić Kovačević, Marina and Pavlović, Slađana and Jeftić, Ilija and Đukić, Aleksandar and Srejović, Ivan and Selaković, Dragica and Jakovljević, Vladimir and Lukić, Miodrag L.",
year = "2021",
abstract = "Galectin 3 (gal 3) has diverse roles in inflammatory and autoimmune diseases. There is evidence that galectin 3 plays a role in both, type 1 and type 2 diabetes. While the role of Gal‐3 expression in immune cells in experimental type 1 diabetes has been already studied, the importance of the overexpression of Gal‐3 in the target β cells is not defined. We used 10‐12 weeks old C57/BL6 male mice (WT) and C57/BL6 mice with transgenically enhanced Gal‐3 expression in pancreatic β cells (TG). Both groups, received STZ for 5 consecutive days at a dose of 40 mg/kg ip. Mice received exogenous mouse IL‐33 (0.4 μg/injection) i.p., 12th, 14 th, 16 th, and 18 th day after the disease induction. Control animals were treated with intraperitoneally PBS + citrate buffer or IL‐33 + citrate buffer. The overexpression of Gal‐3 protected β cells from apoptosis and attenuated MLD‐STZ induced hyperglycemia, glycosuria and ketonuria. The cellular analysis of pancreata and draining lymph nodes showed that Gal‐ 3 overexpression significantly decreased the number of proinflammatory cells without affecting T regulatory cells. The application of exogenous IL‐33, attenuates the development of disease, by increasing the presence of regulatory FoxP3+ ST2+ cells, and competely abrogate diabetogenesis. We demonstrated the potential synergistic effect of exogenous IL‐33 and TG overexpression of Gal‐3 in β cells Not only enhanced expresion of Gal‐3 in β cells reduced T cell mediated autoimmune inflammatory disease, but also exogenous IL‐33 application had powerful terapeutic effect in TG mice.",
publisher = "Wiley‐VCH GmbH",
journal = "6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting",
title = "Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33.",
doi = "10.1002/eji.202170200",
pages = "378"
}

Jovičić, N., Petrović, I., Pejnović, N., Ljujić, B., Miletić Kovačević, M., Pavlović, S., Jeftić, I., Đukić, A., Srejović, I., Selaković, D., Jakovljević, V.,& Lukić, M. L.. (2021). Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33.. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
Wiley‐VCH GmbH., 378.
https://doi.org/10.1002/eji.202170200

Jovičić N, Petrović I, Pejnović N, Ljujić B, Miletić Kovačević M, Pavlović S, Jeftić I, Đukić A, Srejović I, Selaković D, Jakovljević V, Lukić ML. Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33.. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. 2021;:378.
doi:10.1002/eji.202170200 .

Jovičić, Nemanja, Petrović, Ivica, Pejnović, Nada, Ljujić, Biljana, Miletić Kovačević, Marina, Pavlović, Slađana, Jeftić, Ilija, Đukić, Aleksandar, Srejović, Ivan, Selaković, Dragica, Jakovljević, Vladimir, Lukić, Miodrag L., "Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33." in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting (2021):378,
https://doi.org/10.1002/eji.202170200 . .

TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Ajdžanović, Vladimir
AU  - Miljić, Dragana
AU  - Trifunović, Svetlana
AU  - Filipović, Branko
AU  - Stanković, Sanja
AU  - Bolevich, Sergey
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
PY  - 2020
UR  - internal-pdf://ijms-21-02024.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3618
AB  - Estrogen signaling plays an important role in pituitary development and function. In sensitive rat or mice strains of both sexes, estrogen treatments promote lactotropic cell proliferation and induce the formation of pituitary adenomas (dominantly prolactin or growth-hormone-secreting ones). In male patients receiving estrogen, treatment does not necessarily result in pituitary hyperplasia, hyperprolactinemia or adenoma development. In this review, we comprehensively analyze the mechanisms of estrogen action upon their application in male animal models comparing it with available data in human subjects. Sex-specific molecular targets of estrogen action in lactotropic (PRL) cells are highlighted in the context of their proliferative and secretory activity. In addition, putative effects of estradiol on the cellular/tumor microenvironment and the contribution of postnatal pituitary progenitor/stem cells and transdifferentiation processes to prolactinoma development have been analyzed. Finally, estrogen-induced morphological and hormone-secreting changes in pituitary thyrotropic (TSH) and adrenocorticotropic (ACTH) cells are discussed, as well as the putative role of the thyroid and/or glucocorticoid hormones in prolactinoma development, based on the current scarce literature.
T2  - International Journal of Molecular Sciences
T1  - Pituitary Hyperplasia, Hormonal Changes and Prolactinoma Development in Males Exposed to Estrogens — An Insight From Translational Studies
IS  - 6
VL  - 21
DO  - 10.3390/ijms21062024
SP  - 2024
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Ajdžanović, Vladimir and Miljić, Dragana and Trifunović, Svetlana and Filipović, Branko and Stanković, Sanja and Bolevich, Sergey and Jakovljević, Vladimir and Milošević, Verica",
year = "2020",
abstract = "Estrogen signaling plays an important role in pituitary development and function. In sensitive rat or mice strains of both sexes, estrogen treatments promote lactotropic cell proliferation and induce the formation of pituitary adenomas (dominantly prolactin or growth-hormone-secreting ones). In male patients receiving estrogen, treatment does not necessarily result in pituitary hyperplasia, hyperprolactinemia or adenoma development. In this review, we comprehensively analyze the mechanisms of estrogen action upon their application in male animal models comparing it with available data in human subjects. Sex-specific molecular targets of estrogen action in lactotropic (PRL) cells are highlighted in the context of their proliferative and secretory activity. In addition, putative effects of estradiol on the cellular/tumor microenvironment and the contribution of postnatal pituitary progenitor/stem cells and transdifferentiation processes to prolactinoma development have been analyzed. Finally, estrogen-induced morphological and hormone-secreting changes in pituitary thyrotropic (TSH) and adrenocorticotropic (ACTH) cells are discussed, as well as the putative role of the thyroid and/or glucocorticoid hormones in prolactinoma development, based on the current scarce literature.",
journal = "International Journal of Molecular Sciences",
title = "Pituitary Hyperplasia, Hormonal Changes and Prolactinoma Development in Males Exposed to Estrogens — An Insight From Translational Studies",
number = "6",
volume = "21",
doi = "10.3390/ijms21062024",
pages = "2024"
}

Šošić-Jurjević, B., Ajdžanović, V., Miljić, D., Trifunović, S., Filipović, B., Stanković, S., Bolevich, S., Jakovljević, V.,& Milošević, V.. (2020). Pituitary Hyperplasia, Hormonal Changes and Prolactinoma Development in Males Exposed to Estrogens — An Insight From Translational Studies. in International Journal of Molecular Sciences, 21(6), 2024.
https://doi.org/10.3390/ijms21062024

Šošić-Jurjević B, Ajdžanović V, Miljić D, Trifunović S, Filipović B, Stanković S, Bolevich S, Jakovljević V, Milošević V. Pituitary Hyperplasia, Hormonal Changes and Prolactinoma Development in Males Exposed to Estrogens — An Insight From Translational Studies. in International Journal of Molecular Sciences. 2020;21(6):2024.
doi:10.3390/ijms21062024 .

Šošić-Jurjević, Branka , Ajdžanović, Vladimir, Miljić, Dragana, Trifunović, Svetlana, Filipović, Branko, Stanković, Sanja, Bolevich, Sergey, Jakovljević, Vladimir, Milošević, Verica, "Pituitary Hyperplasia, Hormonal Changes and Prolactinoma Development in Males Exposed to Estrogens — An Insight From Translational Studies" in International Journal of Molecular Sciences, 21, no. 6 (2020):2024,
https://doi.org/10.3390/ijms21062024 . .

TY  - JOUR
AU  - Sretenović, Jasmina
AU  - Živković, Vladimir
AU  - Srejović, Ivan
AU  - Ajdžanović, Vladimir
AU  - Ristić, Nataša
AU  - Trifunović, Miroljub
AU  - Pantović, Suzana
AU  - Jović, Slavoljub
AU  - Jakovljević, Vladimir
AU  - Bolevich, Sergey
AU  - Milosavljević, Zoran
AU  - Milošević, Verica
PY  - 2020
UR  - /core/journals/microscopy-and-microanalysis/article/immunohistomorphometric-and-hormonal-analysis-of-the-pituitary-gonadotropic-cells-after-application-of-the-nandrolone-decanoate-and-swimming-training-in-adult-male-rats/CB060C51EF744799F8CC8590E060CAB8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3717
AB  - The aim of the study was to investigate the effects of chronic nandrolone decanoate treatment and/or swimming training on immunohistomorphometric parameters on rat pituitary gonadotropic cells. Male Wistar albino rats, 10 weeks old, were classified into four groups: control (T−N−), nandrolone (T−N+), swimming training (T+N−), and swimming training with nandrolone (T+N+). The T+ groups swam for 4 weeks, 1 h/day, 5 days/week. The N+ groups received nandrolone decanoate (20 mg/kg) once per week for 4 weeks. Pituitary tissue sections were processed and stained for immunohistochemical analysis and immunofluorescence. The volume density of luteinizing hormone (LH) cells was decreased by 48% in T−N+ and for 35% in the T+N+ group. The volume density of follicle-stimulating hormone (FSH) cells was decreased by 39% in T−N+ and for 30% in T+N+ compared to the control. Nandrolone alone, or combined with swimming training, decreased the number of LH/FSH cells compared to the control. The levels of the immunofluorescent signal of LH/FSH cells were increased in all experimental groups. Nandrolone alone decreased the serum level of LH by 17%, whereas swimming training alone increased FSH levels by 11% compared to the control. Serum levels of testosterone were increased in all experimental groups. Nandrolone alone, or combined with swimming training, decreased immunohistomorphometric parameters of gonadotropic cells, whereas the levels of immunofluorescent signal were increased.
PB  - Cambridge University Press (CUP)
T2  - Microscopy and Microanalysis
T1  - Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats
IS  - 4
VL  - 26
DO  - 10.1017/s143192762000166x
SP  - 699
EP  - 707
ER  - 

@article{
author = "Sretenović, Jasmina and Živković, Vladimir and Srejović, Ivan and Ajdžanović, Vladimir and Ristić, Nataša and Trifunović, Miroljub and Pantović, Suzana and Jović, Slavoljub and Jakovljević, Vladimir and Bolevich, Sergey and Milosavljević, Zoran and Milošević, Verica",
year = "2020",
abstract = "The aim of the study was to investigate the effects of chronic nandrolone decanoate treatment and/or swimming training on immunohistomorphometric parameters on rat pituitary gonadotropic cells. Male Wistar albino rats, 10 weeks old, were classified into four groups: control (T−N−), nandrolone (T−N+), swimming training (T+N−), and swimming training with nandrolone (T+N+). The T+ groups swam for 4 weeks, 1 h/day, 5 days/week. The N+ groups received nandrolone decanoate (20 mg/kg) once per week for 4 weeks. Pituitary tissue sections were processed and stained for immunohistochemical analysis and immunofluorescence. The volume density of luteinizing hormone (LH) cells was decreased by 48% in T−N+ and for 35% in the T+N+ group. The volume density of follicle-stimulating hormone (FSH) cells was decreased by 39% in T−N+ and for 30% in T+N+ compared to the control. Nandrolone alone, or combined with swimming training, decreased the number of LH/FSH cells compared to the control. The levels of the immunofluorescent signal of LH/FSH cells were increased in all experimental groups. Nandrolone alone decreased the serum level of LH by 17%, whereas swimming training alone increased FSH levels by 11% compared to the control. Serum levels of testosterone were increased in all experimental groups. Nandrolone alone, or combined with swimming training, decreased immunohistomorphometric parameters of gonadotropic cells, whereas the levels of immunofluorescent signal were increased.",
publisher = "Cambridge University Press (CUP)",
journal = "Microscopy and Microanalysis",
title = "Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats",
number = "4",
volume = "26",
doi = "10.1017/s143192762000166x",
pages = "699-707"
}

Sretenović, J., Živković, V., Srejović, I., Ajdžanović, V., Ristić, N., Trifunović, M., Pantović, S., Jović, S., Jakovljević, V., Bolevich, S., Milosavljević, Z.,& Milošević, V.. (2020). Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats. in Microscopy and Microanalysis
Cambridge University Press (CUP)., 26(4), 699-707.
https://doi.org/10.1017/s143192762000166x

Sretenović J, Živković V, Srejović I, Ajdžanović V, Ristić N, Trifunović M, Pantović S, Jović S, Jakovljević V, Bolevich S, Milosavljević Z, Milošević V. Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats. in Microscopy and Microanalysis. 2020;26(4):699-707.
doi:10.1017/s143192762000166x .

Sretenović, Jasmina, Živković, Vladimir, Srejović, Ivan, Ajdžanović, Vladimir, Ristić, Nataša, Trifunović, Miroljub, Pantović, Suzana, Jović, Slavoljub, Jakovljević, Vladimir, Bolevich, Sergey, Milosavljević, Zoran, Milošević, Verica, "Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats" in Microscopy and Microanalysis, 26, no. 4 (2020):699-707,
https://doi.org/10.1017/s143192762000166x . .

TY  - CONF
AU  - Potrebić, Milica
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Milenković, Dragan
AU  - Pendoski, Laze
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
PY  - 2018
UR  - http://www.enea2018.com/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6041
AB  - Objective: The complex process of aging can be defined as progressive loss of the ability to maintain
tissue homeostasis, which may cause many pathological issues, like depression, Alzheimer’s diseases
or cognitive deficits. Due to their various established health effects, citrus flavanones, naringenin
(NAR) and hesperetin (HES), could have important protective role against development and progression of many age-related diseases.The aim of this study was to examine the effects of these flavanones on pituitary-adrenocortical axis in the rat model of old age (24 months old Wistar rats), Methods: The experimental groups included control group, and naringenin and hesperetin treated groups. NAR and HES were administrated orally (15 mg/kg b. m.) during 4 weeks, while control group received sunflower oil as a vehicle. Concentrations of adrenocorticotropic (ACTH) hormone in pituitary ACTH cells (reflected by relative intensity of fluorescence) and blood levels of ACTH and corticosterone in experimental rats were determined semi-quantitatively and by using the immunoassays, respectively. Results: Results have shown that both blood concentrations of ACTH and corticosterone were markedly lower in NAR [51% and 39%, respectively (P<0.05)] and HES treated group [69% and 65%, respectively (P<0.05)] in comparison with the control group. The relative intensity of ACTH immunofluorescence signal was the highest in the control group, while in NAR and HES groups was significantly lower [39% and 65%, respectively (P<0.05)]. Conclusion: This study has shown that citrus flavanones affect pituitary-adrenocortical axis by decreasing ACTH levels in adrenocorticotropic cells and blood concentrations of ACTH and corticosterone, as well. Since the increased activation of this axis in elderly is associated with development of many aging-related neurophysiological issues, bioactive flavanones may be used in their prevention or delay.
PB  - Altdorf: EndoScience Endokrinologie Service GmbH
C3  - 18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland
T1  - Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats
SP  - 27
EP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6041
ER  - 

@conference{
author = "Potrebić, Milica and Ajdžanović, Vladimir and Miler, Marko and Milenković, Dragan and Pendoski, Laze and Jakovljević, Vladimir and Milošević, Verica",
year = "2018",
abstract = "Objective: The complex process of aging can be defined as progressive loss of the ability to maintain
tissue homeostasis, which may cause many pathological issues, like depression, Alzheimer’s diseases
or cognitive deficits. Due to their various established health effects, citrus flavanones, naringenin
(NAR) and hesperetin (HES), could have important protective role against development and progression of many age-related diseases.The aim of this study was to examine the effects of these flavanones on pituitary-adrenocortical axis in the rat model of old age (24 months old Wistar rats), Methods: The experimental groups included control group, and naringenin and hesperetin treated groups. NAR and HES were administrated orally (15 mg/kg b. m.) during 4 weeks, while control group received sunflower oil as a vehicle. Concentrations of adrenocorticotropic (ACTH) hormone in pituitary ACTH cells (reflected by relative intensity of fluorescence) and blood levels of ACTH and corticosterone in experimental rats were determined semi-quantitatively and by using the immunoassays, respectively. Results: Results have shown that both blood concentrations of ACTH and corticosterone were markedly lower in NAR [51% and 39%, respectively (P<0.05)] and HES treated group [69% and 65%, respectively (P<0.05)] in comparison with the control group. The relative intensity of ACTH immunofluorescence signal was the highest in the control group, while in NAR and HES groups was significantly lower [39% and 65%, respectively (P<0.05)]. Conclusion: This study has shown that citrus flavanones affect pituitary-adrenocortical axis by decreasing ACTH levels in adrenocorticotropic cells and blood concentrations of ACTH and corticosterone, as well. Since the increased activation of this axis in elderly is associated with development of many aging-related neurophysiological issues, bioactive flavanones may be used in their prevention or delay.",
publisher = "Altdorf: EndoScience Endokrinologie Service GmbH",
journal = "18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland",
title = "Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats",
pages = "27-28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6041"
}

Potrebić, M., Ajdžanović, V., Miler, M., Milenković, D., Pendoski, L., Jakovljević, V.,& Milošević, V.. (2018). Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats. in 18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland
Altdorf: EndoScience Endokrinologie Service GmbH., 27-28.
https://hdl.handle.net/21.15107/rcub_ibiss_6041

Potrebić M, Ajdžanović V, Miler M, Milenković D, Pendoski L, Jakovljević V, Milošević V. Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats. in 18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland. 2018;:27-28.
https://hdl.handle.net/21.15107/rcub_ibiss_6041 .

Potrebić, Milica, Ajdžanović, Vladimir, Miler, Marko, Milenković, Dragan, Pendoski, Laze, Jakovljević, Vladimir, Milošević, Verica, "Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats" in 18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland (2018):27-28,
https://hdl.handle.net/21.15107/rcub_ibiss_6041 .

TY  - CONF
AU  - Sretenović, Jasmina
AU  - Živković, Vladimir
AU  - Srejović, Ivan
AU  - Ajdžanović, Vladimir
AU  - Milošević, Verica
AU  - Jakovljević, Vladimir
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5993
AB  - Anabolic androgenic steroids (ASs) are synthetic analogs of testosterone. Because of its anabolic effect, in recent decades, AAS became very popular among professional and recreational sports man. The aim of this study was to examine the chronic effects of exercise training alone or combined with supraphysiological doses of nandrolone decanoate (ND) on cardiac muscle of the left ventricle. The study included 32 Wistar albino male rats, divided into 4 groups: control (I-N-), nandrolone (I-N+), exercise training (T+N-) and exercise training plus nandrolone (T+N+) groups. The T+N- and T+N+ group swam for 3 weeks, 1 hour/day, 5 days/week. The T-N+ and T+N+ groups received nandrolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. After 3 weeks of training, the rats were sacrificed. Heart biopsy specimens were routinely fixed and embedded in paraffin.
Five micrometer thick sections were stained with hematoxylin and eosin (H/E). Captured microscopic images were processed by special software for image analysis to quantify results. Our result show that nandrolone alone causes left ventricular wall thickening for 4% (p > 0.05), training alone 14% (p > 0.05) while training combined with nandrolone leads to increased wall thickening for 24% (p < 0.05). Longitudinal section diameter of cardiac muscle cells was increased for 6% (p >0.05), in T-N+ for 23% (p < 0.05), in T*N- and for 33% (p < 0.05) in T+N+ group. Comparison between the experimental groups showed that the largest difference of longitudinal section diameter can be observed between T-N+ and T+N+ (24%; p < 0.05) and the lowest difference was found between T+N- and T#N+ (8%; p > 0.05). Cross section muscle cell area was increased in T-N+ for 7% (p > 0.05), in T+N- for 29% (p < 0.05), in T+N+ for 31% (p < 0.05). Comparison between the experimental groups showed that the difference of cross section area between T-N+ and T+N+ was 22% (p < 0.05) and the lowest, non-significant difference was found between T+N- and T+N+ (1.6%; p > 0.05). Chronic administration of supraphysiological doses of ND alone promote mild cardiac muscle hypertrophy, but only in combination with the exercise training the degree of the cardiac muscle hypertrophy of the left ventricle is significant.
PB  - Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine
C3  - Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
T1  - Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats
SP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5993
ER  - 

@conference{
author = "Sretenović, Jasmina and Živković, Vladimir and Srejović, Ivan and Ajdžanović, Vladimir and Milošević, Verica and Jakovljević, Vladimir",
year = "2018",
abstract = "Anabolic androgenic steroids (ASs) are synthetic analogs of testosterone. Because of its anabolic effect, in recent decades, AAS became very popular among professional and recreational sports man. The aim of this study was to examine the chronic effects of exercise training alone or combined with supraphysiological doses of nandrolone decanoate (ND) on cardiac muscle of the left ventricle. The study included 32 Wistar albino male rats, divided into 4 groups: control (I-N-), nandrolone (I-N+), exercise training (T+N-) and exercise training plus nandrolone (T+N+) groups. The T+N- and T+N+ group swam for 3 weeks, 1 hour/day, 5 days/week. The T-N+ and T+N+ groups received nandrolone decanoate (20 mg/kg b.w.) once per week, subcutaneously. After 3 weeks of training, the rats were sacrificed. Heart biopsy specimens were routinely fixed and embedded in paraffin.
Five micrometer thick sections were stained with hematoxylin and eosin (H/E). Captured microscopic images were processed by special software for image analysis to quantify results. Our result show that nandrolone alone causes left ventricular wall thickening for 4% (p > 0.05), training alone 14% (p > 0.05) while training combined with nandrolone leads to increased wall thickening for 24% (p < 0.05). Longitudinal section diameter of cardiac muscle cells was increased for 6% (p >0.05), in T-N+ for 23% (p < 0.05), in T*N- and for 33% (p < 0.05) in T+N+ group. Comparison between the experimental groups showed that the largest difference of longitudinal section diameter can be observed between T-N+ and T+N+ (24%; p < 0.05) and the lowest difference was found between T+N- and T#N+ (8%; p > 0.05). Cross section muscle cell area was increased in T-N+ for 7% (p > 0.05), in T+N- for 29% (p < 0.05), in T+N+ for 31% (p < 0.05). Comparison between the experimental groups showed that the difference of cross section area between T-N+ and T+N+ was 22% (p < 0.05) and the lowest, non-significant difference was found between T+N- and T+N+ (1.6%; p > 0.05). Chronic administration of supraphysiological doses of ND alone promote mild cardiac muscle hypertrophy, but only in combination with the exercise training the degree of the cardiac muscle hypertrophy of the left ventricle is significant.",
publisher = "Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine",
journal = "Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia",
title = "Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats",
pages = "150",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5993"
}

Sretenović, J., Živković, V., Srejović, I., Ajdžanović, V., Milošević, V.,& Jakovljević, V.. (2018). Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine., 150.
https://hdl.handle.net/21.15107/rcub_ibiss_5993

Sretenović J, Živković V, Srejović I, Ajdžanović V, Milošević V, Jakovljević V. Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia. 2018;:150.
https://hdl.handle.net/21.15107/rcub_ibiss_5993 .

Sretenović, Jasmina, Živković, Vladimir, Srejović, Ivan, Ajdžanović, Vladimir, Milošević, Verica, Jakovljević, Vladimir, "Morphometric analysis of the left ventricle myocardium after administration of nandrolone decanoate in exercise rats" in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia (2018):150,
https://hdl.handle.net/21.15107/rcub_ibiss_5993 .

TY  - CONF
AU  - Živanović, Jasmina
AU  - Kouroussis, Emilia
AU  - Miler, Marko
AU  - Schott-Roux, Sonia
AU  - Jarić, Ivana
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
AU  - Filipović R., Miloš
PY  - 2018
UR  - http://physiology.org.rs/4thCPS.php
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5926
AB  - An oxidative-posttranslational modification of thiols called protein persulfidation
(RSSH) represents the underlying mechanism of biological actions ascribed to new gasotransmitter hydrogen sulfide (H2S). We developed a new dimedone based method for detection of protein persulfidation and its selectivity was tested and confirmed in various experimental models (D. melanogaster, C. elegans, M. musculus. human cell lines and samples). Obtained results demonstrated that persulfidation represents an evolutionary conserved modification that correlates to the H2S production capacity and is accompanied with numerous physiological implications. One of such general observations is that persulfidation represents protective mechanism for the reduction of sulfenic acids under conditions of oxidative stress. When C. elegans mutants for CSE and MST were exposed to different oxidative stress inducers they showed enhanced sensitivity, while pretreatment with H2S donors rescued them. Additionally, we observed a strong correlation between protein persulfidation levels and development and aging in different experimental models. Fluctuations in the level of persultides were observed during development of C. elegans with prominent increase in L3 larval stage. Our data showed that after strong peak in L3, persulfide levels decreases during life in C. elegans. Decline of persulfide levels in the adult worms of different age was also observed. Similar effect was discovered in the brains of rats of different age. Immunobistological analysis of brain sections for H2S producing enzymes revealed the decrease in intensity of immunopositivity during aging process. Our results strongly propose protein persullidation represents a rescue loop from cysteine hyper-oxidation and subsequent loss of protein function. This general mechanism represents a promising tool in overcoming the consequences of increased oxidative stress during aging process.
PB  - Belgrade: Serbian Physiological Society
C3  - Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences : from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
T1  - Anti-aging properties of protein persulfidation
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5926
ER  - 

@conference{
author = "Živanović, Jasmina and Kouroussis, Emilia and Miler, Marko and Schott-Roux, Sonia and Jarić, Ivana and Jakovljević, Vladimir and Milošević, Verica and Filipović R., Miloš",
year = "2018",
abstract = "An oxidative-posttranslational modification of thiols called protein persulfidation
(RSSH) represents the underlying mechanism of biological actions ascribed to new gasotransmitter hydrogen sulfide (H2S). We developed a new dimedone based method for detection of protein persulfidation and its selectivity was tested and confirmed in various experimental models (D. melanogaster, C. elegans, M. musculus. human cell lines and samples). Obtained results demonstrated that persulfidation represents an evolutionary conserved modification that correlates to the H2S production capacity and is accompanied with numerous physiological implications. One of such general observations is that persulfidation represents protective mechanism for the reduction of sulfenic acids under conditions of oxidative stress. When C. elegans mutants for CSE and MST were exposed to different oxidative stress inducers they showed enhanced sensitivity, while pretreatment with H2S donors rescued them. Additionally, we observed a strong correlation between protein persulfidation levels and development and aging in different experimental models. Fluctuations in the level of persultides were observed during development of C. elegans with prominent increase in L3 larval stage. Our data showed that after strong peak in L3, persulfide levels decreases during life in C. elegans. Decline of persulfide levels in the adult worms of different age was also observed. Similar effect was discovered in the brains of rats of different age. Immunobistological analysis of brain sections for H2S producing enzymes revealed the decrease in intensity of immunopositivity during aging process. Our results strongly propose protein persullidation represents a rescue loop from cysteine hyper-oxidation and subsequent loss of protein function. This general mechanism represents a promising tool in overcoming the consequences of increased oxidative stress during aging process.",
publisher = "Belgrade: Serbian Physiological Society",
journal = "Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences : from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia",
title = "Anti-aging properties of protein persulfidation",
pages = "143",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5926"
}

Živanović, J., Kouroussis, E., Miler, M., Schott-Roux, S., Jarić, I., Jakovljević, V., Milošević, V.,& Filipović R., M.. (2018). Anti-aging properties of protein persulfidation. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences : from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
Belgrade: Serbian Physiological Society., 143.
https://hdl.handle.net/21.15107/rcub_ibiss_5926

Živanović J, Kouroussis E, Miler M, Schott-Roux S, Jarić I, Jakovljević V, Milošević V, Filipović R. M. Anti-aging properties of protein persulfidation. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences : from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia. 2018;:143.
https://hdl.handle.net/21.15107/rcub_ibiss_5926 .

Živanović, Jasmina, Kouroussis, Emilia, Miler, Marko, Schott-Roux, Sonia, Jarić, Ivana, Jakovljević, Vladimir, Milošević, Verica, Filipović R., Miloš, "Anti-aging properties of protein persulfidation" in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences : from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia (2018):143,
https://hdl.handle.net/21.15107/rcub_ibiss_5926 .

TY  - JOUR
AU  - Rosić, Gvozden
AU  - Selaković, Dragica
AU  - Joksimović, Jovana
AU  - Srejović, Ivan
AU  - Živković, Vladimir
AU  - Tatalović, Nikola
AU  - Oreščanin Dušić, Zorana
AU  - Mitrović, Slobodanka
AU  - Ilić, Milena
AU  - Jakovljević, Vladimir
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0378427415301223
UR  - http://www.ncbi.nlm.nih.gov/pubmed/26656795
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2612
AB  - The aim of this study was to evaluate the effects of chronic NAC administration along with cisplatin on cisplatin-induced cardiotoxicity by means of coronary flow (CF), cardiodynamic parameters, oxidative stress markers and morphological changes in isolated rat heart. Isolated hearts of Wistar albino rats (divided into four groups: control, cisplatin, NAC and cisplatin+NAC group) were perfused according to Langendorff technique at constant coronary perfusion pressure starting at 50 and gradually increased to 65, 80, 95 and 110 cm H2O to evaluate cardiodynamic parameters within autoregulation range. Samples of coronary venous effluent (CVE) were collected for determination of CF and biochemical assays, and heart tissue samples for biochemical assays and histopathological examination. Cisplatin treatment decreased CF and heart rate, and increased left ventricular systolic pressure and maximum left ventricular pressure development rate. Cisplatin increased H2O2 and TBARS, but decreased NO2(-) levels in CVE. In tissue samples, cisplatin reduced pathological alterations in myocardium and coronary vessels, with no changes in the amount of total glutathione, as well as in activity of glutathione peroxidase and glutathione reductase. NAC coadministration, by reducing oxidative damage, attenuated cisplatin-induced changes of cardiodynamic and oxidative stress parameters, as well as morphological changes in myocardium and coronary vasculature.
T2  - Toxicology letters
T1  - The effects of N-acetylcysteine on cisplatin-induced changes of cardiodynamic parameters within coronary autoregulation range in isolated rat hearts.
VL  - 242
DO  - 10.1016/j.toxlet.2015.11.028
SP  - 34
EP  - 46
ER  - 

@article{
author = "Rosić, Gvozden and Selaković, Dragica and Joksimović, Jovana and Srejović, Ivan and Živković, Vladimir and Tatalović, Nikola and Oreščanin Dušić, Zorana and Mitrović, Slobodanka and Ilić, Milena and Jakovljević, Vladimir",
year = "2016",
abstract = "The aim of this study was to evaluate the effects of chronic NAC administration along with cisplatin on cisplatin-induced cardiotoxicity by means of coronary flow (CF), cardiodynamic parameters, oxidative stress markers and morphological changes in isolated rat heart. Isolated hearts of Wistar albino rats (divided into four groups: control, cisplatin, NAC and cisplatin+NAC group) were perfused according to Langendorff technique at constant coronary perfusion pressure starting at 50 and gradually increased to 65, 80, 95 and 110 cm H2O to evaluate cardiodynamic parameters within autoregulation range. Samples of coronary venous effluent (CVE) were collected for determination of CF and biochemical assays, and heart tissue samples for biochemical assays and histopathological examination. Cisplatin treatment decreased CF and heart rate, and increased left ventricular systolic pressure and maximum left ventricular pressure development rate. Cisplatin increased H2O2 and TBARS, but decreased NO2(-) levels in CVE. In tissue samples, cisplatin reduced pathological alterations in myocardium and coronary vessels, with no changes in the amount of total glutathione, as well as in activity of glutathione peroxidase and glutathione reductase. NAC coadministration, by reducing oxidative damage, attenuated cisplatin-induced changes of cardiodynamic and oxidative stress parameters, as well as morphological changes in myocardium and coronary vasculature.",
journal = "Toxicology letters",
title = "The effects of N-acetylcysteine on cisplatin-induced changes of cardiodynamic parameters within coronary autoregulation range in isolated rat hearts.",
volume = "242",
doi = "10.1016/j.toxlet.2015.11.028",
pages = "34-46"
}

Rosić, G., Selaković, D., Joksimović, J., Srejović, I., Živković, V., Tatalović, N., Oreščanin Dušić, Z., Mitrović, S., Ilić, M.,& Jakovljević, V.. (2016). The effects of N-acetylcysteine on cisplatin-induced changes of cardiodynamic parameters within coronary autoregulation range in isolated rat hearts.. in Toxicology letters, 242, 34-46.
https://doi.org/10.1016/j.toxlet.2015.11.028

Rosić G, Selaković D, Joksimović J, Srejović I, Živković V, Tatalović N, Oreščanin Dušić Z, Mitrović S, Ilić M, Jakovljević V. The effects of N-acetylcysteine on cisplatin-induced changes of cardiodynamic parameters within coronary autoregulation range in isolated rat hearts.. in Toxicology letters. 2016;242:34-46.
doi:10.1016/j.toxlet.2015.11.028 .

Rosić, Gvozden, Selaković, Dragica, Joksimović, Jovana, Srejović, Ivan, Živković, Vladimir, Tatalović, Nikola, Oreščanin Dušić, Zorana, Mitrović, Slobodanka, Ilić, Milena, Jakovljević, Vladimir, "The effects of N-acetylcysteine on cisplatin-induced changes of cardiodynamic parameters within coronary autoregulation range in isolated rat hearts." in Toxicology letters, 242 (2016):34-46,
https://doi.org/10.1016/j.toxlet.2015.11.028 . .