TY  - JOUR
AU  - Šošić-Jurjević, Branka
AU  - Lütjohann, Dieter
AU  - Trifunović, Svetlana
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
AU  - Jovanović, Ljubiša
AU  - Ristić, Nataša
AU  - Ljiljana, Marina
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6120
AB  - Age and sex influence serum cholesterol levels, but the underlying mechanisms remain
unclear. To investigate further, we measured cholesterol, precursors (surrogate synthesis markers),
degradation products (oxysterols and bile acid precursors) in serum, the liver, jejunum, and ileum,
as well as serum plant sterols (intestinal absorption markers) in male and female Wistar rats (4 and
24 months old). The analysis of histomorphometric and oxidative stress parameters (superoxide
dismutase, catalase, glutathione-related enzyme activities, lipid peroxide, and protein carbonyl concentrations) in the liver and jejunum offered further insights into the age- and sex-related differences.
The hepatic gene expression analysis included AR, ERα, and sex-specific growth hormone-regulated
(Cyp2c11 and Cyp2c12) and thyroid-responsive (Dio1, Tbg, and Spot 14) genes by qPCR. We observed
age-related changes in both sexes, with greater prominence in females. Aged females had significantly
higher serum cholesterol (p < 0.05), jejunum cholesterol (p < 0.05), and serum plant sterols (p < 0.05).
They exhibited poorer hepato-intestinal health compared with males, which was characterized by
mild liver dysfunction (hydropic degeneration, increased serum ALT, p < 0.05, and decreased activity
of some antioxidant defense enzymes, p < 0.05), mononuclear inflammation in the jejunal lamina
propria, and age-related decreases in jejunal catalase and glutathione peroxidase activity (p < 0.05).
Aged females showed increased levels of 27-hydroxycholesterol (p < 0.05) and upregulated ERα gene
expression (p < 0.05) in the liver. Our study suggests that the more significant age-related increase in
serum cholesterol in females is associated with poorer hepato-intestinal health and increased jejunal
cholesterol absorption. The local increase in 27-hydroxycholesterol during aging might reduce the
hepatoprotective effects of endogenous estrogen in the female liver.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age
IS  - 16
VL  - 24
DO  - 10.3390/ijms241612624
SP  - 12624
ER  - 

@article{
author = "Šošić-Jurjević, Branka and Lütjohann, Dieter and Trifunović, Svetlana and Pavlović, Slađan and Borković-Mitić, Slavica and Jovanović, Ljubiša and Ristić, Nataša and Ljiljana, Marina and Ajdžanović, Vladimir and Filipović, Branko",
year = "2023",
abstract = "Age and sex influence serum cholesterol levels, but the underlying mechanisms remain
unclear. To investigate further, we measured cholesterol, precursors (surrogate synthesis markers),
degradation products (oxysterols and bile acid precursors) in serum, the liver, jejunum, and ileum,
as well as serum plant sterols (intestinal absorption markers) in male and female Wistar rats (4 and
24 months old). The analysis of histomorphometric and oxidative stress parameters (superoxide
dismutase, catalase, glutathione-related enzyme activities, lipid peroxide, and protein carbonyl concentrations) in the liver and jejunum offered further insights into the age- and sex-related differences.
The hepatic gene expression analysis included AR, ERα, and sex-specific growth hormone-regulated
(Cyp2c11 and Cyp2c12) and thyroid-responsive (Dio1, Tbg, and Spot 14) genes by qPCR. We observed
age-related changes in both sexes, with greater prominence in females. Aged females had significantly
higher serum cholesterol (p < 0.05), jejunum cholesterol (p < 0.05), and serum plant sterols (p < 0.05).
They exhibited poorer hepato-intestinal health compared with males, which was characterized by
mild liver dysfunction (hydropic degeneration, increased serum ALT, p < 0.05, and decreased activity
of some antioxidant defense enzymes, p < 0.05), mononuclear inflammation in the jejunal lamina
propria, and age-related decreases in jejunal catalase and glutathione peroxidase activity (p < 0.05).
Aged females showed increased levels of 27-hydroxycholesterol (p < 0.05) and upregulated ERα gene
expression (p < 0.05) in the liver. Our study suggests that the more significant age-related increase in
serum cholesterol in females is associated with poorer hepato-intestinal health and increased jejunal
cholesterol absorption. The local increase in 27-hydroxycholesterol during aging might reduce the
hepatoprotective effects of endogenous estrogen in the female liver.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age",
number = "16",
volume = "24",
doi = "10.3390/ijms241612624",
pages = "12624"
}

Šošić-Jurjević, B., Lütjohann, D., Trifunović, S., Pavlović, S., Borković-Mitić, S., Jovanović, L., Ristić, N., Ljiljana, M., Ajdžanović, V.,& Filipović, B.. (2023). Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age. in International Journal of Molecular Sciences
Basel: MDPI., 24(16), 12624.
https://doi.org/10.3390/ijms241612624

Šošić-Jurjević B, Lütjohann D, Trifunović S, Pavlović S, Borković-Mitić S, Jovanović L, Ristić N, Ljiljana M, Ajdžanović V, Filipović B. Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age. in International Journal of Molecular Sciences. 2023;24(16):12624.
doi:10.3390/ijms241612624 .

Šošić-Jurjević, Branka, Lütjohann, Dieter, Trifunović, Svetlana, Pavlović, Slađan, Borković-Mitić, Slavica, Jovanović, Ljubiša, Ristić, Nataša, Ljiljana, Marina, Ajdžanović, Vladimir, Filipović, Branko, "Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age" in International Journal of Molecular Sciences, 24, no. 16 (2023):12624,
https://doi.org/10.3390/ijms241612624 . .

TY  - CONF
AU  - Mitić, Bojan
AU  - Vranković, Jelena
AU  - Stojanović, Dalibor
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6121
AB  - Sex-specific physiology includes a set of physiological traits that differ markedly
between males and females of the same species. In addition to the most obvious
differences between the sexes, such as reproductive roles controlled by sex-specific
endocrine systems, it has been demonstrated that the sexes may also differ in their
oxidative physiology. Centipedes, like other animals, possess a suite of antioxidant
enzymes (AOEs) and non-enzymatic antioxidants that delay, prevent, or remove
oxidative damage to target molecules. The aim of the present study was to
investigate sex-specific differences in the antioxidant defence system in three
centipede species from the Balkan Peninsula.
Here, we measured the activity of the following AOEs: superoxide dismutase
(SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase
(GR), and glutathione S-transferase (GST), as well as the concentrations of sulfhydryl
(SH) groups and glutathione (GSH) in males and females of Eupolybothrus
transsylvanicus, Cryptops anomalans, and Clinopodes flavidus. In addition, we
measured lipid peroxidation (LPO) and protein carbonyl (PCO) concentrations in the
species studied to investigate whether sex-specific trends in the activity of AOEs may
lead to the occurrence of oxidative damage.
E. transsylvanicus, C. anomalans and C. flavidus were collected by hand from
under stones and bark on Ada Ciganlija Island (Belgrade, Serbia) in October 2022.
The sexes were distinguished by external characteristics or by dissection of genital
appendages. The biochemical and statistical analyses were performed following our
previous work in the field.
Overall, the most significant differences were found between E. transsylvanicus
and C. anomalans in the following parameters: SOD, CAT, GR, SH groups, LPO, and
PCO; then between C. anomalans and C. flavidus (GSH-Px, GR, SH groups, LPO, and
PCO), whereas the least significant differences were between E. transsylvanicus and
C. flavidus (GSH-Px only). Besides, SOD, GSH-Px and GSH showed significant
differences between males and females of E. transsylvanicus. In C. anomalans, GSHPx and GSH were significantly divided by sex, while in C. flavidus this was true only
for GST. Moreover, all measured AOEs in E. transsylvanicus had higher values in
males, while in the other two species the situation was reversed – the studied
parameters were higher in females.
Thus, the level of antioxidant defences depends on the species and sex of the
centipedes studied. Our results also suggest that females of centipedes that exhibit
parental care, such as C. anomalans and C. flavidus, have better antioxidant status
than males. This provides a starting point for future research on the evolutionary
causes of sex specificity in the oxidative physiology of centipedes.
PB  - Bogotá: Universidad Nacional De Colombia
C3  - Program and Abstracts:19th International Congress Of Myriapodology; 2023 Aug 7-12; Bogotá, Colombia
T1  - Antioxidant defences in centipedes (Chilopoda): a question of sex
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6121
ER  - 

@conference{
author = "Mitić, Bojan and Vranković, Jelena and Stojanović, Dalibor and Pavlović, Slađan and Borković-Mitić, Slavica",
year = "2023",
abstract = "Sex-specific physiology includes a set of physiological traits that differ markedly
between males and females of the same species. In addition to the most obvious
differences between the sexes, such as reproductive roles controlled by sex-specific
endocrine systems, it has been demonstrated that the sexes may also differ in their
oxidative physiology. Centipedes, like other animals, possess a suite of antioxidant
enzymes (AOEs) and non-enzymatic antioxidants that delay, prevent, or remove
oxidative damage to target molecules. The aim of the present study was to
investigate sex-specific differences in the antioxidant defence system in three
centipede species from the Balkan Peninsula.
Here, we measured the activity of the following AOEs: superoxide dismutase
(SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase
(GR), and glutathione S-transferase (GST), as well as the concentrations of sulfhydryl
(SH) groups and glutathione (GSH) in males and females of Eupolybothrus
transsylvanicus, Cryptops anomalans, and Clinopodes flavidus. In addition, we
measured lipid peroxidation (LPO) and protein carbonyl (PCO) concentrations in the
species studied to investigate whether sex-specific trends in the activity of AOEs may
lead to the occurrence of oxidative damage.
E. transsylvanicus, C. anomalans and C. flavidus were collected by hand from
under stones and bark on Ada Ciganlija Island (Belgrade, Serbia) in October 2022.
The sexes were distinguished by external characteristics or by dissection of genital
appendages. The biochemical and statistical analyses were performed following our
previous work in the field.
Overall, the most significant differences were found between E. transsylvanicus
and C. anomalans in the following parameters: SOD, CAT, GR, SH groups, LPO, and
PCO; then between C. anomalans and C. flavidus (GSH-Px, GR, SH groups, LPO, and
PCO), whereas the least significant differences were between E. transsylvanicus and
C. flavidus (GSH-Px only). Besides, SOD, GSH-Px and GSH showed significant
differences between males and females of E. transsylvanicus. In C. anomalans, GSHPx and GSH were significantly divided by sex, while in C. flavidus this was true only
for GST. Moreover, all measured AOEs in E. transsylvanicus had higher values in
males, while in the other two species the situation was reversed – the studied
parameters were higher in females.
Thus, the level of antioxidant defences depends on the species and sex of the
centipedes studied. Our results also suggest that females of centipedes that exhibit
parental care, such as C. anomalans and C. flavidus, have better antioxidant status
than males. This provides a starting point for future research on the evolutionary
causes of sex specificity in the oxidative physiology of centipedes.",
publisher = "Bogotá: Universidad Nacional De Colombia",
journal = "Program and Abstracts:19th International Congress Of Myriapodology; 2023 Aug 7-12; Bogotá, Colombia",
title = "Antioxidant defences in centipedes (Chilopoda): a question of sex",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6121"
}

Mitić, B., Vranković, J., Stojanović, D., Pavlović, S.,& Borković-Mitić, S.. (2023). Antioxidant defences in centipedes (Chilopoda): a question of sex. in Program and Abstracts:19th International Congress Of Myriapodology; 2023 Aug 7-12; Bogotá, Colombia
Bogotá: Universidad Nacional De Colombia..
https://hdl.handle.net/21.15107/rcub_ibiss_6121

Mitić B, Vranković J, Stojanović D, Pavlović S, Borković-Mitić S. Antioxidant defences in centipedes (Chilopoda): a question of sex. in Program and Abstracts:19th International Congress Of Myriapodology; 2023 Aug 7-12; Bogotá, Colombia. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6121 .

Mitić, Bojan, Vranković, Jelena, Stojanović, Dalibor, Pavlović, Slađan, Borković-Mitić, Slavica, "Antioxidant defences in centipedes (Chilopoda): a question of sex" in Program and Abstracts:19th International Congress Of Myriapodology; 2023 Aug 7-12; Bogotá, Colombia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6121 .

TY  - JOUR
AU  - Mitić, Bojan
AU  - Borković-Mitić, Slavica
AU  - Vranković, Jelena
AU  - Stojanović, Dalibor
AU  - Pavlović, Slađan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5658
AB  - The activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR),
and glutathione S-transferase (GST), as well as the concentrations of sulfhydryl (SH) groups and glutathione (GSH) were
analyzed in five age classes of the Mediterranean centipede Scolopendra cingulata as follows: embryo, adolescens, maturus
junior, maturus, and maturus senior. The data obtained showed the presence of SOD, CAT, GSH-Px, GR, GST, and SH
groups in embryos. The transition from embryo to adolescens was accompanied by an increase in the activities of all studied
enzymes, in response to the increased production of ROS due to the increased metabolic activity of the centipede associated with growth and development. Our results show that trends in antioxidant enzyme (AOE) activities were not uniform
among adult age classes, suggesting that maturus junior, maturus, and maturus senior differentially respond and/or have
different susceptibility to ROS. On the other hand, GSH concentration in embryos was undetectable, highest in adolescens
and decreased in the latter part of life. Pearson correlation analysis in embryos showed that the activities of the AOEs were
strongly and positively correlated with each other but negatively correlated with GSH and SH groups. At later age classes,
SOD, CAT, GSH-Px, GR, GSH, and SH groups were no longer significantly correlated with GST. In the discriminant analysis,
the variables that separated the age classes were GR, GST, SH groups, and body length. Body length was directly related to
the age of individuals, clearly indicating that development/aging affects the regulation of antioxidant defense in this species.
PB  - Heidelberg: Springer
T2  - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
T1  - Age‑related changes in antioxidant defenses of the Mediterranean centipede Scolopendra cingulata (Chilopoda)
DO  - 10.1007/s00360-023-01481-w
ER  - 

@article{
author = "Mitić, Bojan and Borković-Mitić, Slavica and Vranković, Jelena and Stojanović, Dalibor and Pavlović, Slađan",
year = "2023",
abstract = "The activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR),
and glutathione S-transferase (GST), as well as the concentrations of sulfhydryl (SH) groups and glutathione (GSH) were
analyzed in five age classes of the Mediterranean centipede Scolopendra cingulata as follows: embryo, adolescens, maturus
junior, maturus, and maturus senior. The data obtained showed the presence of SOD, CAT, GSH-Px, GR, GST, and SH
groups in embryos. The transition from embryo to adolescens was accompanied by an increase in the activities of all studied
enzymes, in response to the increased production of ROS due to the increased metabolic activity of the centipede associated with growth and development. Our results show that trends in antioxidant enzyme (AOE) activities were not uniform
among adult age classes, suggesting that maturus junior, maturus, and maturus senior differentially respond and/or have
different susceptibility to ROS. On the other hand, GSH concentration in embryos was undetectable, highest in adolescens
and decreased in the latter part of life. Pearson correlation analysis in embryos showed that the activities of the AOEs were
strongly and positively correlated with each other but negatively correlated with GSH and SH groups. At later age classes,
SOD, CAT, GSH-Px, GR, GSH, and SH groups were no longer significantly correlated with GST. In the discriminant analysis,
the variables that separated the age classes were GR, GST, SH groups, and body length. Body length was directly related to
the age of individuals, clearly indicating that development/aging affects the regulation of antioxidant defense in this species.",
publisher = "Heidelberg: Springer",
journal = "Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology",
title = "Age‑related changes in antioxidant defenses of the Mediterranean centipede Scolopendra cingulata (Chilopoda)",
doi = "10.1007/s00360-023-01481-w"
}

Mitić, B., Borković-Mitić, S., Vranković, J., Stojanović, D.,& Pavlović, S.. (2023). Age‑related changes in antioxidant defenses of the Mediterranean centipede Scolopendra cingulata (Chilopoda). in Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
Heidelberg: Springer..
https://doi.org/10.1007/s00360-023-01481-w

Mitić B, Borković-Mitić S, Vranković J, Stojanović D, Pavlović S. Age‑related changes in antioxidant defenses of the Mediterranean centipede Scolopendra cingulata (Chilopoda). in Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology. 2023;.
doi:10.1007/s00360-023-01481-w .

Mitić, Bojan, Borković-Mitić, Slavica, Vranković, Jelena, Stojanović, Dalibor, Pavlović, Slađan, "Age‑related changes in antioxidant defenses of the Mediterranean centipede Scolopendra cingulata (Chilopoda)" in Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology (2023),
https://doi.org/10.1007/s00360-023-01481-w . .

TY  - JOUR
AU  - Manojlović-Stojanoski, Milica
AU  - Borković Mitić, Slavica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Trifunović, Svetlana
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Stojsavljević, Aleksandar
AU  - Pavlović, Slađan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5198
AB  - The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) and inorganic sodium-selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy.  Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females  started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta
IS  - 21
VL  - 23
DO  - 10.3390/ijms232113068
SP  - 13068
ER  - 

@article{
author = "Manojlović-Stojanoski, Milica and Borković Mitić, Slavica and Nestorović, Nataša and Ristić, Nataša and Trifunović, Svetlana and Stevanović, Magdalena and Filipović, Nenad and Stojsavljević, Aleksandar and Pavlović, Slađan",
year = "2022",
abstract = "The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) and inorganic sodium-selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy.  Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females  started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta",
number = "21",
volume = "23",
doi = "10.3390/ijms232113068",
pages = "13068"
}

Manojlović-Stojanoski, M., Borković Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A.,& Pavlović, S.. (2022). The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta. in International Journal of Molecular Sciences
Basel: MDPI., 23(21), 13068.
https://doi.org/10.3390/ijms232113068

Manojlović-Stojanoski M, Borković Mitić S, Nestorović N, Ristić N, Trifunović S, Stevanović M, Filipović N, Stojsavljević A, Pavlović S. The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta. in International Journal of Molecular Sciences. 2022;23(21):13068.
doi:10.3390/ijms232113068 .

Manojlović-Stojanoski, Milica, Borković Mitić, Slavica, Nestorović, Nataša, Ristić, Nataša, Trifunović, Svetlana, Stevanović, Magdalena, Filipović, Nenad, Stojsavljević, Aleksandar, Pavlović, Slađan, "The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta" in International Journal of Molecular Sciences, 23, no. 21 (2022):13068,
https://doi.org/10.3390/ijms232113068 . .

TY  - JOUR
AU  - Jagodić, Jovana
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Perović, Milan
AU  - Miković, Željko
AU  - Đurđić, Slađana
AU  - Manojlović, Dragan
AU  - Stojsavljević, Aleksandar
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5020
AB  - With the ever-growing concern for human health and wellbeing, the prenatal period of
development requires special attention since fetuses can be exposed to various metals through the
mother. Therefore, this study explored the status of selected toxic (Pb, Cd, Ni, As, Pt, Ce, Rb, Sr, U)
and essential trace metals (Mn, Co, Cu, Zn, Se) in the umbilical cord (UC) sera, maternal sera, and
placental tissue samples of 92 healthy women with normal pregnancies. A further aim focuses on the
potential transplacental transfer of these trace metals. Based on the obtained levels of investigated
elements in clinical samples, it was observed that all of the trace metals cross the placental barrier
and reach the fetus. Furthermore, statistical analysis revealed significant differences in levels of
toxic Ni, As, Cd, U, Sr, Rb, and essential Mn, Cu, and Zn between all three types of analyzed clinical
samples. Correlation analysis highlighted As to be an element with levels that differed significantly
between all tested samples. Principal component analysis (PCA) was used to enhance these findings.
PCA demonstrated that Cd, Mn, Zn, Rb, Ce, U, and Sr were the most influential trace metals in
distinguishing placenta from maternal and UC serum samples. As, Co, and Cu were responsible for
the clustering of maternal serum samples, and PCA demonstrated that the Pt level in UC sera was
responsible for the clustering of these samples. Overall, the findings of this study could contribute to
a better understanding of transplacental transfer of these trace metals, and shed a light on overall
levels of metal exposure in the population of healthy pregnant women and their fetuses.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy
IS  - 15
VL  - 23
DO  - 10.3390/ijms23158078
SP  - 8078
ER  - 

@article{
author = "Jagodić, Jovana and Pavlović, Slađan and Borković Mitić, Slavica and Perović, Milan and Miković, Željko and Đurđić, Slađana and Manojlović, Dragan and Stojsavljević, Aleksandar",
year = "2022",
abstract = "With the ever-growing concern for human health and wellbeing, the prenatal period of
development requires special attention since fetuses can be exposed to various metals through the
mother. Therefore, this study explored the status of selected toxic (Pb, Cd, Ni, As, Pt, Ce, Rb, Sr, U)
and essential trace metals (Mn, Co, Cu, Zn, Se) in the umbilical cord (UC) sera, maternal sera, and
placental tissue samples of 92 healthy women with normal pregnancies. A further aim focuses on the
potential transplacental transfer of these trace metals. Based on the obtained levels of investigated
elements in clinical samples, it was observed that all of the trace metals cross the placental barrier
and reach the fetus. Furthermore, statistical analysis revealed significant differences in levels of
toxic Ni, As, Cd, U, Sr, Rb, and essential Mn, Cu, and Zn between all three types of analyzed clinical
samples. Correlation analysis highlighted As to be an element with levels that differed significantly
between all tested samples. Principal component analysis (PCA) was used to enhance these findings.
PCA demonstrated that Cd, Mn, Zn, Rb, Ce, U, and Sr were the most influential trace metals in
distinguishing placenta from maternal and UC serum samples. As, Co, and Cu were responsible for
the clustering of maternal serum samples, and PCA demonstrated that the Pt level in UC sera was
responsible for the clustering of these samples. Overall, the findings of this study could contribute to
a better understanding of transplacental transfer of these trace metals, and shed a light on overall
levels of metal exposure in the population of healthy pregnant women and their fetuses.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy",
number = "15",
volume = "23",
doi = "10.3390/ijms23158078",
pages = "8078"
}

Jagodić, J., Pavlović, S., Borković Mitić, S., Perović, M., Miković, Ž., Đurđić, S., Manojlović, D.,& Stojsavljević, A.. (2022). Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy. in International Journal of Molecular Sciences
Basel: MDPI., 23(15), 8078.
https://doi.org/10.3390/ijms23158078

Jagodić J, Pavlović S, Borković Mitić S, Perović M, Miković Ž, Đurđić S, Manojlović D, Stojsavljević A. Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy. in International Journal of Molecular Sciences. 2022;23(15):8078.
doi:10.3390/ijms23158078 .

Jagodić, Jovana, Pavlović, Slađan, Borković Mitić, Slavica, Perović, Milan, Miković, Željko, Đurđić, Slađana, Manojlović, Dragan, Stojsavljević, Aleksandar, "Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy" in International Journal of Molecular Sciences, 23, no. 15 (2022):8078,
https://doi.org/10.3390/ijms23158078 . .

TY  - CONF
AU  - Manojlović-Stojanoski, Milica
AU  - Borković-Mitić, Slavica
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Trifunović, Svetlana
AU  - Stevanović, Magdalena
AU  - Pavlović, Slađan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5319
AB  - Селен (Se) је металоид неопходан за правилно функционисање организма. Улази у
састав више од 25 селенопротеина. Неопходан је за антиоксидативни систем
(АОС), метаболизам тиреоидних хормона, синтезу ДНК, плодност, имунитет…
Концентрације Se у организму варирају у малом опсегу од чега зависи његов
дефицит, оптималан биолошки учинак или токсичност.
1 Селен продире у
организам у неорганском (селенит или селенат) и органском (селенометионин и
селеноцистеин) облику. Органски облици селена показују најбољу
биорасположивост.2
Биомедицински ефекти честица нано селена (SeNPs) су
интензивно истраживани током протекле деценије.3 Циљ ове студије био је да се
упореде ефекти SeNPs и Na-селенита на АОС у плаценти гравидних женки пацова.
Плацента је посредник између мајке и фетуса и учествује у транспорту Se током
целог интраутериног развоја. Гравидне женке Wistar albino пацова су гаважом
свакога дана добијале SeNPs или Na-селенит. Испитивана је активност супероксид
дисмутазе (SOD), каталазе (CAT), глутатион пероксидазе (GSH-Px), глутатион
редуктазе (GR) и глутатион С-трансферазе (GST), као и концентрације глутатиона
(GSH) и сулфхидрилних група (SH). SeNPs је довео до значајног повећања
активности SOD, CAT и GSH-Px и смањења активности GST и концентрација GSH
и SH у плаценти, док је третман Na-селенитом довео до повећања концентрације
GSH у односу на контроле. Добијени резултати указују да SeNPs и Na-селенит
изазивају различит физиолошки ефекат у плаценти гравидних женки пацова, а
даља истраживања треба да утврде да ли је у питању повећано оксидативно
оптерећење или побољшана антиоксидативна заштита.
AB  - Selen (Se) je metaloid neophodan za pravilno funkcionisanje organizma. Ulazi u sastav više od 25 selenoproteina. Neophodan je za antioksidativni sistem (AOS), metabolizam tireoidnih hormona, sintezu DNK, plodnost, imunitet… Koncentracije Se u organizmu variraju u malom opsegu od čega zavisi njegov deficit, optimalan biološki učinak ili toksičnost. 1 Selen prodire u organizam u neorganskom (selenit ili selenat) i organskom (selenometionin i selenocistein) obliku. Organski oblici selena pokazuju najbolju bioraspoloživost.2 Biomedicinski efekti čestica nano selena (SeNPs) su intenzivno istraživani tokom protekle decenije.3 Cilj ove studije bio je da se uporede efekti SeNPs i Na-selenita na AOS u placenti gravidnih ženki pacova. Placenta je posrednik između majke i fetusa i učestvuje u transportu Se tokom celog intrauterinog razvoja. Gravidne ženke Wistar albino pacova su gavažom svakoga dana dobijale SeNPs ili Na-selenit. Ispitivana je aktivnost superoksid dismutaze (SOD), katalaze (CAT), glutation peroksidaze (GSH-Px), glutation reduktaze (GR) i glutation S-transferaze (GST), kao i koncentracije glutationa (GSH) i sulfhidrilnih grupa (SH). SeNPs je doveo do značajnog povećanja aktivnosti SOD, CAT i GSH-Px i smanjenja aktivnosti GST i koncentracija GSH i SH u placenti, dok je tretman Na-selenitom doveo do povećanja koncentracije GSH u odnosu na kontrole. Dobijeni rezultati ukazuju da SeNPs i Na-selenit izazivaju različit fiziološki efekat u placenti gravidnih ženki pacova, a dalja istraživanja treba da utvrde da li je u pitanju povećano oksidativno opterećenje ili poboljšana antioksidativna zaštita.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Efekti različitih formi selena na na antioksidativni status placente pacova
T1  - Ефекти различитих форми селена на антиоксидативни статус плаценте пацова
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5319
ER  - 

@conference{
author = "Manojlović-Stojanoski, Milica and Borković-Mitić, Slavica and Ristić, Nataša and Nestorović, Nataša and Trifunović, Svetlana and Stevanović, Magdalena and Pavlović, Slađan",
year = "2022",
abstract = "Селен (Se) је металоид неопходан за правилно функционисање организма. Улази у
састав више од 25 селенопротеина. Неопходан је за антиоксидативни систем
(АОС), метаболизам тиреоидних хормона, синтезу ДНК, плодност, имунитет…
Концентрације Se у организму варирају у малом опсегу од чега зависи његов
дефицит, оптималан биолошки учинак или токсичност.
1 Селен продире у
организам у неорганском (селенит или селенат) и органском (селенометионин и
селеноцистеин) облику. Органски облици селена показују најбољу
биорасположивост.2
Биомедицински ефекти честица нано селена (SeNPs) су
интензивно истраживани током протекле деценије.3 Циљ ове студије био је да се
упореде ефекти SeNPs и Na-селенита на АОС у плаценти гравидних женки пацова.
Плацента је посредник између мајке и фетуса и учествује у транспорту Se током
целог интраутериног развоја. Гравидне женке Wistar albino пацова су гаважом
свакога дана добијале SeNPs или Na-селенит. Испитивана је активност супероксид
дисмутазе (SOD), каталазе (CAT), глутатион пероксидазе (GSH-Px), глутатион
редуктазе (GR) и глутатион С-трансферазе (GST), као и концентрације глутатиона
(GSH) и сулфхидрилних група (SH). SeNPs је довео до значајног повећања
активности SOD, CAT и GSH-Px и смањења активности GST и концентрација GSH
и SH у плаценти, док је третман Na-селенитом довео до повећања концентрације
GSH у односу на контроле. Добијени резултати указују да SeNPs и Na-селенит
изазивају различит физиолошки ефекат у плаценти гравидних женки пацова, а
даља истраживања треба да утврде да ли је у питању повећано оксидативно
оптерећење или побољшана антиоксидативна заштита., Selen (Se) je metaloid neophodan za pravilno funkcionisanje organizma. Ulazi u sastav više od 25 selenoproteina. Neophodan je za antioksidativni sistem (AOS), metabolizam tireoidnih hormona, sintezu DNK, plodnost, imunitet… Koncentracije Se u organizmu variraju u malom opsegu od čega zavisi njegov deficit, optimalan biološki učinak ili toksičnost. 1 Selen prodire u organizam u neorganskom (selenit ili selenat) i organskom (selenometionin i selenocistein) obliku. Organski oblici selena pokazuju najbolju bioraspoloživost.2 Biomedicinski efekti čestica nano selena (SeNPs) su intenzivno istraživani tokom protekle decenije.3 Cilj ove studije bio je da se uporede efekti SeNPs i Na-selenita na AOS u placenti gravidnih ženki pacova. Placenta je posrednik između majke i fetusa i učestvuje u transportu Se tokom celog intrauterinog razvoja. Gravidne ženke Wistar albino pacova su gavažom svakoga dana dobijale SeNPs ili Na-selenit. Ispitivana je aktivnost superoksid dismutaze (SOD), katalaze (CAT), glutation peroksidaze (GSH-Px), glutation reduktaze (GR) i glutation S-transferaze (GST), kao i koncentracije glutationa (GSH) i sulfhidrilnih grupa (SH). SeNPs je doveo do značajnog povećanja aktivnosti SOD, CAT i GSH-Px i smanjenja aktivnosti GST i koncentracija GSH i SH u placenti, dok je tretman Na-selenitom doveo do povećanja koncentracije GSH u odnosu na kontrole. Dobijeni rezultati ukazuju da SeNPs i Na-selenit izazivaju različit fiziološki efekat u placenti gravidnih ženki pacova, a dalja istraživanja treba da utvrde da li je u pitanju povećano oksidativno opterećenje ili poboljšana antioksidativna zaštita.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Efekti različitih formi selena na na antioksidativni status placente pacova, Ефекти различитих форми селена на антиоксидативни статус плаценте пацова",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5319"
}

Manojlović-Stojanoski, M., Borković-Mitić, S., Ristić, N., Nestorović, N., Trifunović, S., Stevanović, M.,& Pavlović, S.. (2022). Efekti različitih formi selena na na antioksidativni status placente pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society..
https://hdl.handle.net/21.15107/rcub_ibiss_5319

Manojlović-Stojanoski M, Borković-Mitić S, Ristić N, Nestorović N, Trifunović S, Stevanović M, Pavlović S. Efekti različitih formi selena na na antioksidativni status placente pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5319 .

Manojlović-Stojanoski, Milica, Borković-Mitić, Slavica, Ristić, Nataša, Nestorović, Nataša, Trifunović, Svetlana, Stevanović, Magdalena, Pavlović, Slađan, "Efekti različitih formi selena na na antioksidativni status placente pacova" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5319 .

TY  - CONF
AU  - Lakić, Iva
AU  - Đurašević, Siniša
AU  - Ružičić, Aleksandra
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Glumac, Sofija
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
AU  - Grigorov, Ilijana
AU  - Stanković, Sanja
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena
AU  - Todorović, Zoran
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5933
AB  - Сепса је стање опасно по живот узроковано нерегулисаним и прекомерним
одговором на инфекцију, које праћено инфламацијом и поремећеним
метаболизмом липида доводи до постепеног отказивања органа.1,2 Мелдонијум је
антиинфламаторни лек који се користи за лечење исхемије миокарда.3 Он делује
тако што преусмерава производњу енергије са оксидације масних киселина на
гликолизу, као пут који троши мање кисеоника. На овај начин мелдонијум
негативно утиче на метаболизам липида. Истраживали смо ефекте
четворонедељног претретмана мелдонијумом на ток фекалне сепсе (ФИП) и сепсе
изазиване липополисахаридом (ЛПС), код мужјака пацова. Изненађујуће, у
условима фекалне сепсе, мелдонијум је повећао стопу морталитета животиња у
поређењу са групом која није третирана мелдонијумом. Анализе оксидативног и
инфламаторног статуса ткива (срце, јетра, бубрези) потврдиле су
антиинфламаторне, антиапоптотске и антинекротичке ефекте мелдонијума, код оба
модела сепсе. Када је реч о производњи енергије, упркос неким сличностима,
утврђене су разлике у ова два модела. Једна од очигледних разлика је у одговору
симпатоадреналног система, који је изостао код ФИП модела, док је код ЛПС
модела довео до двоструког повећања концентрације катехоламина у серуму. Ова
студија показује важност ненарушене производње енергије скрећући пажњу на
потребу ревизије постојећих смерница у клиничком лечењу сепсе, али и отвара пут
за откривање нових терапијских приступа.
AB  - Sepsa je stanje opasno po život uzrokovano neregulisanim i prekomernim odgovorom na infekciju, koje praćeno inflamacijom i poremećenim metabolizmom lipida dovodi do postepenog otkazivanja organa.1,2 Meldonijum je antiinflamatorni lek koji se koristi za lečenje ishemije miokarda.3 On deluje tako što preusmerava proizvodnju energije sa oksidacije masnih kiselina na glikolizu, kao put koji troši manje kiseonika. Na ovaj način meldonijum negativno utiče na metabolizam lipida. Istraživali smo efekte četvoronedeljnog pretretmana meldonijumom na tok fekalne sepse (FIP) i sepse izazivane lipopolisaharidom (LPS), kod mužjaka pacova. Iznenađujuće, u uslovima fekalne sepse, meldonijum je povećao stopu mortaliteta životinja u poređenju sa grupom koja nije tretirana meldonijumom. Analize oksidativnog i inflamatornog statusa tkiva (srce, jetra, bubrezi) potvrdile su antiinflamatorne, antiapoptotske i antinekrotičke efekte meldonijuma, kod oba modela sepse. Kada je reč o proizvodnji energije, uprkos nekim sličnostima, utvrđene su razlike u ova dva modela. Jedna od očiglednih razlika je u odgovoru simpatoadrenalnog sistema, koji je izostao kod FIP modela, dok je kod LPS modela doveo do dvostrukog povećanja koncentracije kateholamina u serumu. Ova studija pokazuje važnost nenarušene proizvodnje energije skrećući pažnju na potrebu revizije postojećih smernica u kliničkom lečenju sepse, ali i otvara put za otkrivanje novih terapijskih pristupa.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Efekti pretretmana meldonijumom na tokove različitih modela sepsi kod pacova
T1  - Ефекти претретмана мелдонијумом на токове различитих модела сепси код пацова
SP  - 340
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5933
ER  - 

@conference{
author = "Lakić, Iva and Đurašević, Siniša and Ružičić, Aleksandra and Tosti, Tomislav and Đurović, Saša and Glumac, Sofija and Pavlović, Slađan and Borković-Mitić, Slavica and Grigorov, Ilijana and Stanković, Sanja and Pejić, Snežana and Todorović, Ana and Drakulić, Dunja and Jasnić, Nebojša and Đorđević, Jelena and Todorović, Zoran",
year = "2022",
abstract = "Сепса је стање опасно по живот узроковано нерегулисаним и прекомерним
одговором на инфекцију, које праћено инфламацијом и поремећеним
метаболизмом липида доводи до постепеног отказивања органа.1,2 Мелдонијум је
антиинфламаторни лек који се користи за лечење исхемије миокарда.3 Он делује
тако што преусмерава производњу енергије са оксидације масних киселина на
гликолизу, као пут који троши мање кисеоника. На овај начин мелдонијум
негативно утиче на метаболизам липида. Истраживали смо ефекте
четворонедељног претретмана мелдонијумом на ток фекалне сепсе (ФИП) и сепсе
изазиване липополисахаридом (ЛПС), код мужјака пацова. Изненађујуће, у
условима фекалне сепсе, мелдонијум је повећао стопу морталитета животиња у
поређењу са групом која није третирана мелдонијумом. Анализе оксидативног и
инфламаторног статуса ткива (срце, јетра, бубрези) потврдиле су
антиинфламаторне, антиапоптотске и антинекротичке ефекте мелдонијума, код оба
модела сепсе. Када је реч о производњи енергије, упркос неким сличностима,
утврђене су разлике у ова два модела. Једна од очигледних разлика је у одговору
симпатоадреналног система, који је изостао код ФИП модела, док је код ЛПС
модела довео до двоструког повећања концентрације катехоламина у серуму. Ова
студија показује важност ненарушене производње енергије скрећући пажњу на
потребу ревизије постојећих смерница у клиничком лечењу сепсе, али и отвара пут
за откривање нових терапијских приступа., Sepsa je stanje opasno po život uzrokovano neregulisanim i prekomernim odgovorom na infekciju, koje praćeno inflamacijom i poremećenim metabolizmom lipida dovodi do postepenog otkazivanja organa.1,2 Meldonijum je antiinflamatorni lek koji se koristi za lečenje ishemije miokarda.3 On deluje tako što preusmerava proizvodnju energije sa oksidacije masnih kiselina na glikolizu, kao put koji troši manje kiseonika. Na ovaj način meldonijum negativno utiče na metabolizam lipida. Istraživali smo efekte četvoronedeljnog pretretmana meldonijumom na tok fekalne sepse (FIP) i sepse izazivane lipopolisaharidom (LPS), kod mužjaka pacova. Iznenađujuće, u uslovima fekalne sepse, meldonijum je povećao stopu mortaliteta životinja u poređenju sa grupom koja nije tretirana meldonijumom. Analize oksidativnog i inflamatornog statusa tkiva (srce, jetra, bubrezi) potvrdile su antiinflamatorne, antiapoptotske i antinekrotičke efekte meldonijuma, kod oba modela sepse. Kada je reč o proizvodnji energije, uprkos nekim sličnostima, utvrđene su razlike u ova dva modela. Jedna od očiglednih razlika je u odgovoru simpatoadrenalnog sistema, koji je izostao kod FIP modela, dok je kod LPS modela doveo do dvostrukog povećanja koncentracije kateholamina u serumu. Ova studija pokazuje važnost nenarušene proizvodnje energije skrećući pažnju na potrebu revizije postojećih smernica u kliničkom lečenju sepse, ali i otvara put za otkrivanje novih terapijskih pristupa.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Efekti pretretmana meldonijumom na tokove različitih modela sepsi kod pacova, Ефекти претретмана мелдонијумом на токове различитих модела сепси код пацова",
pages = "340",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5933"
}

Lakić, I., Đurašević, S., Ružičić, A., Tosti, T., Đurović, S., Glumac, S., Pavlović, S., Borković-Mitić, S., Grigorov, I., Stanković, S., Pejić, S., Todorović, A., Drakulić, D., Jasnić, N., Đorđević, J.,& Todorović, Z.. (2022). Efekti pretretmana meldonijumom na tokove različitih modela sepsi kod pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 340.
https://hdl.handle.net/21.15107/rcub_ibiss_5933

Lakić I, Đurašević S, Ružičić A, Tosti T, Đurović S, Glumac S, Pavlović S, Borković-Mitić S, Grigorov I, Stanković S, Pejić S, Todorović A, Drakulić D, Jasnić N, Đorđević J, Todorović Z. Efekti pretretmana meldonijumom na tokove različitih modela sepsi kod pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:340.
https://hdl.handle.net/21.15107/rcub_ibiss_5933 .

Lakić, Iva, Đurašević, Siniša, Ružičić, Aleksandra, Tosti, Tomislav, Đurović, Saša, Glumac, Sofija, Pavlović, Slađan, Borković-Mitić, Slavica, Grigorov, Ilijana, Stanković, Sanja, Pejić, Snežana, Todorović, Ana, Drakulić, Dunja, Jasnić, Nebojša, Đorđević, Jelena, Todorović, Zoran, "Efekti pretretmana meldonijumom na tokove različitih modela sepsi kod pacova" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):340,
https://hdl.handle.net/21.15107/rcub_ibiss_5933 .

TY  - CONF
AU  - Borković-Mitić, Slavica
AU  - Mitić, Bojan
AU  - Vranković, Jelena
AU  - Stojanović, Dalibor
AU  - Pavlović, Slađan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5932
AB  - Антиоксидативни ензими су важан механизам одбране од реактивних врста
кисеоника (ROS) и, као и многи други биохемијски системи, њихова ефикасност
може да варира у зависности од фазе развића и других физиолошких аспеката
организма. У овој студији испитивали смо промене у антиоксидативној одбрани
током развића медитеранске врсте стоноге Scolopendra cingulata (Chilopoda). Попут
других бескичмењака који живе у аеробним условима, и ова стонога поседује
кључне антиоксидативне ензиме – супероксид дисмутазу (SOD), каталазу (CAT),
глутатион пероксидазу (GSH-Px), глутатион редуктазу (GR) и глутатион Sтрансферазу (GST). Активност ових ензима је била најнижа у ембрионалној фази, а
њено значајно повећање током ране постембриогенезе учинило је слободноживеће
јединке (стадијум адолесценс) спремнијим за суочавање са условима у којима
настаје већа количина ROS. Наши резултати су показали да промена активности
антиоксидативних ензима, приликом преласка из јувенилне у адултну фазу
(матурус јуниор, матурус и матурус сениор), је вероватно последица бројних
развојних процеса интезивираних у том стадијуму. Важно је да је поред
физиолошких и фактора средине, онтогенетски развој такође одлучујући фактор
нивоа антиоксидативних ензима код ових животиња.
AB  - Antioksidativni enzimi su važan mehanizam odbrane od reaktivnih vrsta kiseonika (ROS) i, kao i mnogi drugi biohemijski sistemi, njihova efikasnost može da varira u zavisnosti od faze razvića i drugih fizioloških aspekata organizma. U ovoj studiji ispitivali smo promene u antioksidativnoj odbrani tokom razvića mediteranske vrste stonoge Scolopendra cingulata (Chilopoda). Poput drugih beskičmenjaka koji žive u aerobnim uslovima, i ova stonoga poseduje ključne antioksidativne enzime – superoksid dismutazu (SOD), katalazu (CAT), glutation peroksidazu (GSH-Px), glutation reduktazu (GR) i glutation Stransferazu (GST). Aktivnost ovih enzima je bila najniža u embrionalnoj fazi, a njeno značajno povećanje tokom rane postembriogeneze učinilo je slobodnoživeće jedinke (stadijum adolescens) spremnijim za suočavanje sa uslovima u kojima nastaje veća količina ROS. Naši rezultati su pokazali da promena aktivnosti antioksidativnih enzima, prilikom prelaska iz juvenilne u adultnu fazu (maturus junior, maturus i maturus senior), je verovatno posledica brojnih razvojnih procesa inteziviranih u tom stadijumu. Važno je da je pored fizioloških i faktora sredine, ontogenetski razvoj takođe odlučujući faktor nivoa antioksidativnih enzima kod ovih životinja.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Промене активности антиоксидативног система током развића стоноге Scolopendra cingulata (Chilopoda)
T1  - Promene aktivnosti antioksidativnog sistema tokom razvića stonoge Scolopendra cingulata (Chilopoda)
SP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5932
ER  - 

@conference{
author = "Borković-Mitić, Slavica and Mitić, Bojan and Vranković, Jelena and Stojanović, Dalibor and Pavlović, Slađan",
year = "2022",
abstract = "Антиоксидативни ензими су важан механизам одбране од реактивних врста
кисеоника (ROS) и, као и многи други биохемијски системи, њихова ефикасност
може да варира у зависности од фазе развића и других физиолошких аспеката
организма. У овој студији испитивали смо промене у антиоксидативној одбрани
током развића медитеранске врсте стоноге Scolopendra cingulata (Chilopoda). Попут
других бескичмењака који живе у аеробним условима, и ова стонога поседује
кључне антиоксидативне ензиме – супероксид дисмутазу (SOD), каталазу (CAT),
глутатион пероксидазу (GSH-Px), глутатион редуктазу (GR) и глутатион Sтрансферазу (GST). Активност ових ензима је била најнижа у ембрионалној фази, а
њено значајно повећање током ране постембриогенезе учинило је слободноживеће
јединке (стадијум адолесценс) спремнијим за суочавање са условима у којима
настаје већа количина ROS. Наши резултати су показали да промена активности
антиоксидативних ензима, приликом преласка из јувенилне у адултну фазу
(матурус јуниор, матурус и матурус сениор), је вероватно последица бројних
развојних процеса интезивираних у том стадијуму. Важно је да је поред
физиолошких и фактора средине, онтогенетски развој такође одлучујући фактор
нивоа антиоксидативних ензима код ових животиња., Antioksidativni enzimi su važan mehanizam odbrane od reaktivnih vrsta kiseonika (ROS) i, kao i mnogi drugi biohemijski sistemi, njihova efikasnost može da varira u zavisnosti od faze razvića i drugih fizioloških aspekata organizma. U ovoj studiji ispitivali smo promene u antioksidativnoj odbrani tokom razvića mediteranske vrste stonoge Scolopendra cingulata (Chilopoda). Poput drugih beskičmenjaka koji žive u aerobnim uslovima, i ova stonoga poseduje ključne antioksidativne enzime – superoksid dismutazu (SOD), katalazu (CAT), glutation peroksidazu (GSH-Px), glutation reduktazu (GR) i glutation Stransferazu (GST). Aktivnost ovih enzima je bila najniža u embrionalnoj fazi, a njeno značajno povećanje tokom rane postembriogeneze učinilo je slobodnoživeće jedinke (stadijum adolescens) spremnijim za suočavanje sa uslovima u kojima nastaje veća količina ROS. Naši rezultati su pokazali da promena aktivnosti antioksidativnih enzima, prilikom prelaska iz juvenilne u adultnu fazu (maturus junior, maturus i maturus senior), je verovatno posledica brojnih razvojnih procesa inteziviranih u tom stadijumu. Važno je da je pored fizioloških i faktora sredine, ontogenetski razvoj takođe odlučujući faktor nivoa antioksidativnih enzima kod ovih životinja.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Промене активности антиоксидативног система током развића стоноге Scolopendra cingulata (Chilopoda), Promene aktivnosti antioksidativnog sistema tokom razvića stonoge Scolopendra cingulata (Chilopoda)",
pages = "176",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5932"
}

Borković-Mitić, S., Mitić, B., Vranković, J., Stojanović, D.,& Pavlović, S.. (2022). Промене активности антиоксидативног система током развића стоноге Scolopendra cingulata (Chilopoda). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 176.
https://hdl.handle.net/21.15107/rcub_ibiss_5932

Borković-Mitić S, Mitić B, Vranković J, Stojanović D, Pavlović S. Промене активности антиоксидативног система током развића стоноге Scolopendra cingulata (Chilopoda). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:176.
https://hdl.handle.net/21.15107/rcub_ibiss_5932 .

Borković-Mitić, Slavica, Mitić, Bojan, Vranković, Jelena, Stojanović, Dalibor, Pavlović, Slađan, "Промене активности антиоксидативног система током развића стоноге Scolopendra cingulata (Chilopoda)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):176,
https://hdl.handle.net/21.15107/rcub_ibiss_5932 .

TY  - JOUR
AU  - Mitić, Bojan
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Sojanović, Dalibor
AU  - Pavlović, Slađan
AU  - Vasiljević, Ljubica
AU  - Ristić, Nataša
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5044
AB  - Three centipede species (Clinopodes flavidus,Cryptops anomalans and Eupolybothrus transsylvanicus) were used as bioindicators of trace metal and metalloid pollution in Belgrade, Serbia. The concentrations of 13 elements (the metals Mn, Co, Ni, Cu, Zn, Rb,Sr,Cd, Tl, Pb and U and metalloids As,Se) in whole animals and soil were measured by inductively coupled plasma mass spectrometry (ICP-MS). Differences in the concentrations of some elements in the analyzed species were observed, both in response to the sites and between species. 1n most cases, the trace element concentrations were higher in centipedes from a polluted site (an industrial area near a busy street with heavy traffic) but C. anomalans and E. transsylvanicus had higher Mn concentrations at an unpolluted site (a deciduous woodland on Mt. Avala). C. flavidus was a good bioindicator for detecting differences between Zn, Se and Cd. C. flavidus and C. anomalans were more efficient in accumulating Zn than E. transsylvanicus. It appears that C. anomalans poorly accumulated Cd, unlike C. flavidus and E. transsylvanicus, which accumulated Cd according to the high bioaccwnulation factor (BAF) values. We conclude that the centipedes C. flavidus, C. anomalans and E. transsylvanicus can be used as suitable bioindicators of trace element exposure. Their ability to accumulate trace elements was different and depends on their physiology and lifestyle as well as the route of exposure.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Metal and Metalloid bioaccumulation in three centipedes (Chilopoda)
IS  - 3
VL  - 74
DO  - 10.2298/ABS220514019M
SP  - 207
EP  - 215
ER  - 

@article{
author = "Mitić, Bojan and Borković Mitić, Slavica and Stojsavljević, Aleksandar and Sojanović, Dalibor and Pavlović, Slađan and Vasiljević, Ljubica and Ristić, Nataša",
year = "2022",
abstract = "Three centipede species (Clinopodes flavidus,Cryptops anomalans and Eupolybothrus transsylvanicus) were used as bioindicators of trace metal and metalloid pollution in Belgrade, Serbia. The concentrations of 13 elements (the metals Mn, Co, Ni, Cu, Zn, Rb,Sr,Cd, Tl, Pb and U and metalloids As,Se) in whole animals and soil were measured by inductively coupled plasma mass spectrometry (ICP-MS). Differences in the concentrations of some elements in the analyzed species were observed, both in response to the sites and between species. 1n most cases, the trace element concentrations were higher in centipedes from a polluted site (an industrial area near a busy street with heavy traffic) but C. anomalans and E. transsylvanicus had higher Mn concentrations at an unpolluted site (a deciduous woodland on Mt. Avala). C. flavidus was a good bioindicator for detecting differences between Zn, Se and Cd. C. flavidus and C. anomalans were more efficient in accumulating Zn than E. transsylvanicus. It appears that C. anomalans poorly accumulated Cd, unlike C. flavidus and E. transsylvanicus, which accumulated Cd according to the high bioaccwnulation factor (BAF) values. We conclude that the centipedes C. flavidus, C. anomalans and E. transsylvanicus can be used as suitable bioindicators of trace element exposure. Their ability to accumulate trace elements was different and depends on their physiology and lifestyle as well as the route of exposure.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Metal and Metalloid bioaccumulation in three centipedes (Chilopoda)",
number = "3",
volume = "74",
doi = "10.2298/ABS220514019M",
pages = "207-215"
}

Mitić, B., Borković Mitić, S., Stojsavljević, A., Sojanović, D., Pavlović, S., Vasiljević, L.,& Ristić, N.. (2022). Metal and Metalloid bioaccumulation in three centipedes (Chilopoda). in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 74(3), 207-215.
https://doi.org/10.2298/ABS220514019M

Mitić B, Borković Mitić S, Stojsavljević A, Sojanović D, Pavlović S, Vasiljević L, Ristić N. Metal and Metalloid bioaccumulation in three centipedes (Chilopoda). in Archives of Biological Sciences. 2022;74(3):207-215.
doi:10.2298/ABS220514019M .

Mitić, Bojan, Borković Mitić, Slavica, Stojsavljević, Aleksandar, Sojanović, Dalibor, Pavlović, Slađan, Vasiljević, Ljubica, Ristić, Nataša, "Metal and Metalloid bioaccumulation in three centipedes (Chilopoda)" in Archives of Biological Sciences, 74, no. 3 (2022):207-215,
https://doi.org/10.2298/ABS220514019M . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Stojković, Maja
AU  - Sopta, Jelena
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Ivanović, Anđelija
AU  - Jasnić, Nebojša
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Đorđević, Jelena
AU  - Todorović, Zoran
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4090
AB  - Acute ischemia/reperfusion (I/R) liver injury is a clinical condition challenging to treat. Meldonium
is an anti‑ischemic agent that shifts energy production from fatty acid oxidation to less oxygen‑
consuming glycolysis. Thus, we investigated the effects of a 4‑week meldonium pre‑treatment
(300 mg/kg b.m./day) on the acute I/R liver injury in Wistar strain male rats. Our results showed that
meldonium ameliorates I/R‑induced liver inflammation and injury, as confirmed by liver histology,
and by attenuation of serum alanine‑ and aspartate aminotransferase activity, serum and liver
high mobility group box 1 protein expression, and liver expression of Bax/Bcl2, haptoglobin, and
the phosphorylated nuclear factor kappa‑light‑chain‑enhancer of activated B cells. Through the
increased hepatic activation of the nuclear factor erythroid 2‑related factor 2, meldonium improves
the antioxidative defence in the liver of animals subjected to I/R, as proved by an increase in serum
and liver ascorbic/dehydroascorbic acid ratio, hepatic haem oxygenase 1 expression, glutathione
and free thiol groups content, and hepatic copper‑zinc superoxide dismutase, manganese superoxide
dismutase, catalase, glutathione peroxidase, and glutathione reductase activity. Based on our results,
it can be concluded that meldonium represent a protective agent against I/R‑induced liver injury, with
a clinical significance in surgical procedures.
PB  - Nature Publishing Group
T2  - Scientific Reports
T1  - The effects of meldonium on the acute ischemia/reperfusion liver injury in rats
IS  - 1
VL  - 11
DO  - 10.1038/s41598-020-80011-y
SP  - 1305
ER  - 

@article{
author = "Đurašević, Siniša and Stojković, Maja and Sopta, Jelena and Pavlović, Slađan and Borković Mitić, Slavica and Ivanović, Anđelija and Jasnić, Nebojša and Tosti, Tomislav and Đurović, Saša and Đorđević, Jelena and Todorović, Zoran",
year = "2021",
abstract = "Acute ischemia/reperfusion (I/R) liver injury is a clinical condition challenging to treat. Meldonium
is an anti‑ischemic agent that shifts energy production from fatty acid oxidation to less oxygen‑
consuming glycolysis. Thus, we investigated the effects of a 4‑week meldonium pre‑treatment
(300 mg/kg b.m./day) on the acute I/R liver injury in Wistar strain male rats. Our results showed that
meldonium ameliorates I/R‑induced liver inflammation and injury, as confirmed by liver histology,
and by attenuation of serum alanine‑ and aspartate aminotransferase activity, serum and liver
high mobility group box 1 protein expression, and liver expression of Bax/Bcl2, haptoglobin, and
the phosphorylated nuclear factor kappa‑light‑chain‑enhancer of activated B cells. Through the
increased hepatic activation of the nuclear factor erythroid 2‑related factor 2, meldonium improves
the antioxidative defence in the liver of animals subjected to I/R, as proved by an increase in serum
and liver ascorbic/dehydroascorbic acid ratio, hepatic haem oxygenase 1 expression, glutathione
and free thiol groups content, and hepatic copper‑zinc superoxide dismutase, manganese superoxide
dismutase, catalase, glutathione peroxidase, and glutathione reductase activity. Based on our results,
it can be concluded that meldonium represent a protective agent against I/R‑induced liver injury, with
a clinical significance in surgical procedures.",
publisher = "Nature Publishing Group",
journal = "Scientific Reports",
title = "The effects of meldonium on the acute ischemia/reperfusion liver injury in rats",
number = "1",
volume = "11",
doi = "10.1038/s41598-020-80011-y",
pages = "1305"
}

Đurašević, S., Stojković, M., Sopta, J., Pavlović, S., Borković Mitić, S., Ivanović, A., Jasnić, N., Tosti, T., Đurović, S., Đorđević, J.,& Todorović, Z.. (2021). The effects of meldonium on the acute ischemia/reperfusion liver injury in rats. in Scientific Reports
Nature Publishing Group., 11(1), 1305.
https://doi.org/10.1038/s41598-020-80011-y

Đurašević S, Stojković M, Sopta J, Pavlović S, Borković Mitić S, Ivanović A, Jasnić N, Tosti T, Đurović S, Đorđević J, Todorović Z. The effects of meldonium on the acute ischemia/reperfusion liver injury in rats. in Scientific Reports. 2021;11(1):1305.
doi:10.1038/s41598-020-80011-y .

Đurašević, Siniša, Stojković, Maja, Sopta, Jelena, Pavlović, Slađan, Borković Mitić, Slavica, Ivanović, Anđelija, Jasnić, Nebojša, Tosti, Tomislav, Đurović, Saša, Đorđević, Jelena, Todorović, Zoran, "The effects of meldonium on the acute ischemia/reperfusion liver injury in rats" in Scientific Reports, 11, no. 1 (2021):1305,
https://doi.org/10.1038/s41598-020-80011-y . .

TY  - JOUR
AU  - Jagodić, Jovana
AU  - Rovčanin, Branislav
AU  - Borković Mitić, Slavica
AU  - Vujotić, Ljiljana
AU  - Avdin, Viacheslav
AU  - Manojlović, Dragan
AU  - Stojsavljević, Aleksandar
PY  - 2021
UR  - https://doi.org/10.1007/s11356-021-14013-2
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4236
AB  - Studies indicate that the soil, water and consequently foodstuffs in Serbia are significantly poor in zinc (Zn), and thus, it is likely that there is a Zn deficiency in the Serbian population. This study examined the Zn status in multiple clinical samples, including body fluids (serum, cerebrospinal fluid), whole blood and Zn-rich solid tissues (thyroid and brain tissue). Differences between sex and age were also considered, and comparative analysis of Zn status with other world populations was performed. Serum samples from a large number of Serbian adults approximately had twofold lower Zn amounts when compared to other populations. A similar trend was obtained for whole blood. Males had significantly higher amounts of Zn in serum, whole blood and thyroid tissue samples than females. Higher amounts of Zn were observed in the group older than 50 years. Importantly, in thyroid and brain tissues, Zn was 10- and 20-fold lower, respectively, than reported in the literature. Our results indicate that the population in Serbia could be considered Zn deficient. Therefore, adequate oral Zn supplementation and/or foodstuff fortification should be considered to prevent the deleterious effects caused by Zn deficiency.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Environmental Science and Pollution Research
T1  - Possible zinc deficiency in the Serbian population: examination of body fluids, whole blood and solid tissues
IS  - 34
VL  - 28
DO  - 10.1007/s11356-021-14013-2
SP  - 47439
EP  - 47446
ER  - 

@article{
author = "Jagodić, Jovana and Rovčanin, Branislav and Borković Mitić, Slavica and Vujotić, Ljiljana and Avdin, Viacheslav and Manojlović, Dragan and Stojsavljević, Aleksandar",
year = "2021",
abstract = "Studies indicate that the soil, water and consequently foodstuffs in Serbia are significantly poor in zinc (Zn), and thus, it is likely that there is a Zn deficiency in the Serbian population. This study examined the Zn status in multiple clinical samples, including body fluids (serum, cerebrospinal fluid), whole blood and Zn-rich solid tissues (thyroid and brain tissue). Differences between sex and age were also considered, and comparative analysis of Zn status with other world populations was performed. Serum samples from a large number of Serbian adults approximately had twofold lower Zn amounts when compared to other populations. A similar trend was obtained for whole blood. Males had significantly higher amounts of Zn in serum, whole blood and thyroid tissue samples than females. Higher amounts of Zn were observed in the group older than 50 years. Importantly, in thyroid and brain tissues, Zn was 10- and 20-fold lower, respectively, than reported in the literature. Our results indicate that the population in Serbia could be considered Zn deficient. Therefore, adequate oral Zn supplementation and/or foodstuff fortification should be considered to prevent the deleterious effects caused by Zn deficiency.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Environmental Science and Pollution Research",
title = "Possible zinc deficiency in the Serbian population: examination of body fluids, whole blood and solid tissues",
number = "34",
volume = "28",
doi = "10.1007/s11356-021-14013-2",
pages = "47439-47446"
}

Jagodić, J., Rovčanin, B., Borković Mitić, S., Vujotić, L., Avdin, V., Manojlović, D.,& Stojsavljević, A.. (2021). Possible zinc deficiency in the Serbian population: examination of body fluids, whole blood and solid tissues. in Environmental Science and Pollution Research
Springer Science and Business Media Deutschland GmbH., 28(34), 47439-47446.
https://doi.org/10.1007/s11356-021-14013-2

Jagodić J, Rovčanin B, Borković Mitić S, Vujotić L, Avdin V, Manojlović D, Stojsavljević A. Possible zinc deficiency in the Serbian population: examination of body fluids, whole blood and solid tissues. in Environmental Science and Pollution Research. 2021;28(34):47439-47446.
doi:10.1007/s11356-021-14013-2 .

Jagodić, Jovana, Rovčanin, Branislav, Borković Mitić, Slavica, Vujotić, Ljiljana, Avdin, Viacheslav, Manojlović, Dragan, Stojsavljević, Aleksandar, "Possible zinc deficiency in the Serbian population: examination of body fluids, whole blood and solid tissues" in Environmental Science and Pollution Research, 28, no. 34 (2021):47439-47446,
https://doi.org/10.1007/s11356-021-14013-2 . .

TY  - JOUR
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Vujotić, Ljiljana
AU  - Matić, Siniša
AU  - Mitić, Bojan
AU  - Manojlović, Dragan
AU  - Pavlović, Slađan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4344
AB  - There are only a few reports examining the impact of oxidative stress in patients with benign and
malignant brain tumors. In this study we investigated whether there are changes in antioxidant
system (AOS) parameters and key trace elements between control, benign and malignant brain
tissues. The study also aimed to examine correlations between the analyzed parameters. The study
enrolled both types of brain tumors, benign tumors (BT) and malignant tumors (MT). The results
were compared with control tissue (CT) without tumor infiltration collected from patients with BT.
The following antioxidant parameters were determined: activities of total, manganese‑containing,
and copper/zinc‑containing superoxide dismutase (TotSOD, MnSOD and CuZnSOD), activities of
catalase, glutathione peroxidase, glutathione S‑transferase, glutathione reductase and acetylcholine
esterase (AChE), the concentrations of glutathione and sulfhydryl groups and of manganese (Mn),
copper (Cu), zinc (Zn), and selenium (Se). BT and MT had altered activities/levels of multiple AOS
parameters as compared to CT, indicating that tumor cells had an altered cell metabolism and changes
in AOS represent adaptive response to increased oxidative stress. Low MnSOD and AChE and high
GST activities were significant for distinguishing between MT and CT. Malignant tissue was also
characterized by lower Mn and Cu concentrations relative to CT and BT. Principal Component Analysis
clearly discriminated BT from CT and MT (PC1, 66.97%), while PC2 clearly discriminated CT from BT
and MT (33.03%). Most correlative relationships were associated with Se in the BT group and Cu in the
MT group. The results of this study reveal differences between the AOS parameters and the essential
trace elements between the analyzed groups. The observed dysregulations show that oxidative stress
could have an important role in disrupting brain homeostasis and its presence in the pathogenesis of
benign and malignant brain tumors.
PB  - Springer Nature
T2  - Scientifc Reports
T1  - Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues
VL  - 11
DO  - 10.1038/s41598-021-94302-5
SP  - 14776
ER  - 

@article{
author = "Borković Mitić, Slavica and Stojsavljević, Aleksandar and Vujotić, Ljiljana and Matić, Siniša and Mitić, Bojan and Manojlović, Dragan and Pavlović, Slađan",
year = "2021",
abstract = "There are only a few reports examining the impact of oxidative stress in patients with benign and
malignant brain tumors. In this study we investigated whether there are changes in antioxidant
system (AOS) parameters and key trace elements between control, benign and malignant brain
tissues. The study also aimed to examine correlations between the analyzed parameters. The study
enrolled both types of brain tumors, benign tumors (BT) and malignant tumors (MT). The results
were compared with control tissue (CT) without tumor infiltration collected from patients with BT.
The following antioxidant parameters were determined: activities of total, manganese‑containing,
and copper/zinc‑containing superoxide dismutase (TotSOD, MnSOD and CuZnSOD), activities of
catalase, glutathione peroxidase, glutathione S‑transferase, glutathione reductase and acetylcholine
esterase (AChE), the concentrations of glutathione and sulfhydryl groups and of manganese (Mn),
copper (Cu), zinc (Zn), and selenium (Se). BT and MT had altered activities/levels of multiple AOS
parameters as compared to CT, indicating that tumor cells had an altered cell metabolism and changes
in AOS represent adaptive response to increased oxidative stress. Low MnSOD and AChE and high
GST activities were significant for distinguishing between MT and CT. Malignant tissue was also
characterized by lower Mn and Cu concentrations relative to CT and BT. Principal Component Analysis
clearly discriminated BT from CT and MT (PC1, 66.97%), while PC2 clearly discriminated CT from BT
and MT (33.03%). Most correlative relationships were associated with Se in the BT group and Cu in the
MT group. The results of this study reveal differences between the AOS parameters and the essential
trace elements between the analyzed groups. The observed dysregulations show that oxidative stress
could have an important role in disrupting brain homeostasis and its presence in the pathogenesis of
benign and malignant brain tumors.",
publisher = "Springer Nature",
journal = "Scientifc Reports",
title = "Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues",
volume = "11",
doi = "10.1038/s41598-021-94302-5",
pages = "14776"
}

Borković Mitić, S., Stojsavljević, A., Vujotić, L., Matić, S., Mitić, B., Manojlović, D.,& Pavlović, S.. (2021). Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues. in Scientifc Reports
Springer Nature., 11, 14776.
https://doi.org/10.1038/s41598-021-94302-5

Borković Mitić S, Stojsavljević A, Vujotić L, Matić S, Mitić B, Manojlović D, Pavlović S. Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues. in Scientifc Reports. 2021;11:14776.
doi:10.1038/s41598-021-94302-5 .

Borković Mitić, Slavica, Stojsavljević, Aleksandar, Vujotić, Ljiljana, Matić, Siniša, Mitić, Bojan, Manojlović, Dragan, Pavlović, Slađan, "Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues" in Scientifc Reports, 11 (2021):14776,
https://doi.org/10.1038/s41598-021-94302-5 . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Ružičić, Aleksandra
AU  - Lakić, Iva
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Glumac, Sofija
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Grigorov, Ilijana
AU  - Stanković, Sanja
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena
AU  - Todorović, Zoran
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4413
AB  - Sepsis is a life-threatening condition caused by the dysregulated and overwhelming
response to infection, accompanied by an exaggerated pro-inflammatory state and lipid metabolism
disturbance leading to sequential organ failure. Meldonium is an anti-ischemic and anti-inflammatory
agent which negatively interferes with lipid metabolism by shifting energy production from fatty
acid oxidation to glycolysis, as a less oxygen-demanding pathway. Thus, we investigated the effects
of a four-week meldonium pre-treatment on faecal-induced sepsis in Sprague-Dawley male rats.
Surprisingly, under septic conditions, meldonium increased animal mortality rate compared with
the meldonium non-treated group. However, analysis of the tissue oxidative status did not provide
support for the detrimental effects of meldonium, nor did the analysis of the tissue inflammatory
status showing anti-inflammatory, anti-apoptotic, and anti-necrotic effects of meldonium. After
performing tissue lipidomic analysis, we concluded that the potential cause of the meldonium
harmful effect is to be found in the overall decreased lipid metabolism. The present study underlines
the importance of uninterrupted energy production in sepsis, closely drawing attention to the possible
harmful effects of lipid-mobilization impairment caused by certain therapeutics. This could lead to
the much-needed revision of the existing guidelines in the clinical treatment of sepsis while paving
the way for discovering new therapeutic approaches.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - The effects of a meldonium pre-treatment on the course of the faecal-induced sepsis in rats
IS  - 18
VL  - 22
DO  - 10.3390/ijms22189698
SP  - 9698
ER  - 

@article{
author = "Đurašević, Siniša and Ružičić, Aleksandra and Lakić, Iva and Tosti, Tomislav and Đurović, Saša and Glumac, Sofija and Pavlović, Slađan and Borković Mitić, Slavica and Grigorov, Ilijana and Stanković, Sanja and Jasnić, Nebojša and Đorđević, Jelena and Todorović, Zoran",
year = "2021",
abstract = "Sepsis is a life-threatening condition caused by the dysregulated and overwhelming
response to infection, accompanied by an exaggerated pro-inflammatory state and lipid metabolism
disturbance leading to sequential organ failure. Meldonium is an anti-ischemic and anti-inflammatory
agent which negatively interferes with lipid metabolism by shifting energy production from fatty
acid oxidation to glycolysis, as a less oxygen-demanding pathway. Thus, we investigated the effects
of a four-week meldonium pre-treatment on faecal-induced sepsis in Sprague-Dawley male rats.
Surprisingly, under septic conditions, meldonium increased animal mortality rate compared with
the meldonium non-treated group. However, analysis of the tissue oxidative status did not provide
support for the detrimental effects of meldonium, nor did the analysis of the tissue inflammatory
status showing anti-inflammatory, anti-apoptotic, and anti-necrotic effects of meldonium. After
performing tissue lipidomic analysis, we concluded that the potential cause of the meldonium
harmful effect is to be found in the overall decreased lipid metabolism. The present study underlines
the importance of uninterrupted energy production in sepsis, closely drawing attention to the possible
harmful effects of lipid-mobilization impairment caused by certain therapeutics. This could lead to
the much-needed revision of the existing guidelines in the clinical treatment of sepsis while paving
the way for discovering new therapeutic approaches.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "The effects of a meldonium pre-treatment on the course of the faecal-induced sepsis in rats",
number = "18",
volume = "22",
doi = "10.3390/ijms22189698",
pages = "9698"
}

Đurašević, S., Ružičić, A., Lakić, I., Tosti, T., Đurović, S., Glumac, S., Pavlović, S., Borković Mitić, S., Grigorov, I., Stanković, S., Jasnić, N., Đorđević, J.,& Todorović, Z.. (2021). The effects of a meldonium pre-treatment on the course of the faecal-induced sepsis in rats. in International Journal of Molecular Sciences
Basel: MDPI., 22(18), 9698.
https://doi.org/10.3390/ijms22189698

Đurašević S, Ružičić A, Lakić I, Tosti T, Đurović S, Glumac S, Pavlović S, Borković Mitić S, Grigorov I, Stanković S, Jasnić N, Đorđević J, Todorović Z. The effects of a meldonium pre-treatment on the course of the faecal-induced sepsis in rats. in International Journal of Molecular Sciences. 2021;22(18):9698.
doi:10.3390/ijms22189698 .

Đurašević, Siniša, Ružičić, Aleksandra, Lakić, Iva, Tosti, Tomislav, Đurović, Saša, Glumac, Sofija, Pavlović, Slađan, Borković Mitić, Slavica, Grigorov, Ilijana, Stanković, Sanja, Jasnić, Nebojša, Đorđević, Jelena, Todorović, Zoran, "The effects of a meldonium pre-treatment on the course of the faecal-induced sepsis in rats" in International Journal of Molecular Sciences, 22, no. 18 (2021):9698,
https://doi.org/10.3390/ijms22189698 . .

TY  - CONF
AU  - Lakić, Iva
AU  - Đurašević, Siniša
AU  - Ružičić, Aleksandra
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Glumac, Sofija
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Grigorov, Ilijana
AU  - Stanković, Sanja
AU  - Jasnić, Nebojša
AU  - Todorović, Zoran
AU  - Đorđević, Jelena
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4896
AB  - Background: Sepsis is a life-threatening condition caused by the dysregulated and overwhelming response to infection, accompanied by exaggerated pro-inflammatory state and lipid metabolism disturbance leading to sequential organ failure.1, 2 Meldonium is an anti-ischemic and anti-inflammatory agent, clinically used to treat myocardial ischemia.3 By shifting energy production from fatty acid oxidation to glycolysis, as an oxygen less consuming pathway, meldonium interferes negatively with lipid metabolism. 
Methods: Thus, we investigated the effects of a 4-week meldonium pre-treatment in 300 mg/kg b.m./day dosage on the course of the sepsis induced by a single intraperitoneal injection of faeces (0.5 g faeces/1 mL saline/100 g b.m.)in Sprague-Dawley male rats. The degree of the heart injury was evaluated by measuring tissue pro-apoptotic Bax and anti-apoptotic Bcl-2 ratio, tissue level of the necrotic marker - high mobility group box 1 protein level (HMGB1), together with the heart histology analysis. Sepsis-associated heart inflammation
was assessed by measuring level of an activated form of NF-kB p65 (phospho-NF-κB p65).
Results: In the heart whole homogenates of the septic group of animals (S) HMGB1 level increased 1.7-fold, in comparison to control rats, while meldonium reduced sepsis-induced increase by 18 % (M+S). The underlying mechanism of the proinflammatory action of HMGB1 includes activation of NF-κB signalling
pathways tissue, so the increased HMGB1 level was followed by a 1.4-fold increase of p-NF-κB p65 in the heart of the S group of rats and a 19 % decreased in the heart of M+S group. The apoptotic marker Bax/Bcl-2 ratio changed in the same manner: 1.4-fold increase in the heart of animals from the S group and a 32 % decrease in the heart of the M+S group. On the other hand, heart histology analysis shows that meldonium worsened the heart histological score, causing the severe and diffuse interstitial mononuclear infiltration along with a greater loss of myocytes and myofibrillar contraction band necrosis. The heart lipidomic analysis suggests that meldonium exhibits potentially harmful effects under septic condition due to the lipid-mobilization impairment.
Conclusion: Meldonium exerted anti-inflammatory, anti-apoptotic, and anti- necrotic effects, while it worsened the septic rat heart histology.
PB  - Banja Luka: Association of Medical Doctors
PB  - Banja Luka: Faculty of Medicine, University of Banja Luka
C3  - 7th Meeting of the European section and 8th Meeting of the North American section of the International academy of cardiovascular sciences (IACS); 2021 Sep 20-23; Banja Luka, Bosnia and Herzegovina
T1  - The Effects of a Meldonium Pre-Treatment on the Sepsis-Induced Rat Heart Injury
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4896
ER  - 

@conference{
author = "Lakić, Iva and Đurašević, Siniša and Ružičić, Aleksandra and Tosti, Tomislav and Đurović, Saša and Glumac, Sofija and Pavlović, Slađan and Borković Mitić, Slavica and Grigorov, Ilijana and Stanković, Sanja and Jasnić, Nebojša and Todorović, Zoran and Đorđević, Jelena",
year = "2021",
abstract = "Background: Sepsis is a life-threatening condition caused by the dysregulated and overwhelming response to infection, accompanied by exaggerated pro-inflammatory state and lipid metabolism disturbance leading to sequential organ failure.1, 2 Meldonium is an anti-ischemic and anti-inflammatory agent, clinically used to treat myocardial ischemia.3 By shifting energy production from fatty acid oxidation to glycolysis, as an oxygen less consuming pathway, meldonium interferes negatively with lipid metabolism. 
Methods: Thus, we investigated the effects of a 4-week meldonium pre-treatment in 300 mg/kg b.m./day dosage on the course of the sepsis induced by a single intraperitoneal injection of faeces (0.5 g faeces/1 mL saline/100 g b.m.)in Sprague-Dawley male rats. The degree of the heart injury was evaluated by measuring tissue pro-apoptotic Bax and anti-apoptotic Bcl-2 ratio, tissue level of the necrotic marker - high mobility group box 1 protein level (HMGB1), together with the heart histology analysis. Sepsis-associated heart inflammation
was assessed by measuring level of an activated form of NF-kB p65 (phospho-NF-κB p65).
Results: In the heart whole homogenates of the septic group of animals (S) HMGB1 level increased 1.7-fold, in comparison to control rats, while meldonium reduced sepsis-induced increase by 18 % (M+S). The underlying mechanism of the proinflammatory action of HMGB1 includes activation of NF-κB signalling
pathways tissue, so the increased HMGB1 level was followed by a 1.4-fold increase of p-NF-κB p65 in the heart of the S group of rats and a 19 % decreased in the heart of M+S group. The apoptotic marker Bax/Bcl-2 ratio changed in the same manner: 1.4-fold increase in the heart of animals from the S group and a 32 % decrease in the heart of the M+S group. On the other hand, heart histology analysis shows that meldonium worsened the heart histological score, causing the severe and diffuse interstitial mononuclear infiltration along with a greater loss of myocytes and myofibrillar contraction band necrosis. The heart lipidomic analysis suggests that meldonium exhibits potentially harmful effects under septic condition due to the lipid-mobilization impairment.
Conclusion: Meldonium exerted anti-inflammatory, anti-apoptotic, and anti- necrotic effects, while it worsened the septic rat heart histology.",
publisher = "Banja Luka: Association of Medical Doctors, Banja Luka: Faculty of Medicine, University of Banja Luka",
journal = "7th Meeting of the European section and 8th Meeting of the North American section of the International academy of cardiovascular sciences (IACS); 2021 Sep 20-23; Banja Luka, Bosnia and Herzegovina",
title = "The Effects of a Meldonium Pre-Treatment on the Sepsis-Induced Rat Heart Injury",
pages = "57",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4896"
}

Lakić, I., Đurašević, S., Ružičić, A., Tosti, T., Đurović, S., Glumac, S., Pavlović, S., Borković Mitić, S., Grigorov, I., Stanković, S., Jasnić, N., Todorović, Z.,& Đorđević, J.. (2021). The Effects of a Meldonium Pre-Treatment on the Sepsis-Induced Rat Heart Injury. in 7th Meeting of the European section and 8th Meeting of the North American section of the International academy of cardiovascular sciences (IACS); 2021 Sep 20-23; Banja Luka, Bosnia and Herzegovina
Banja Luka: Association of Medical Doctors., 57.
https://hdl.handle.net/21.15107/rcub_ibiss_4896

Lakić I, Đurašević S, Ružičić A, Tosti T, Đurović S, Glumac S, Pavlović S, Borković Mitić S, Grigorov I, Stanković S, Jasnić N, Todorović Z, Đorđević J. The Effects of a Meldonium Pre-Treatment on the Sepsis-Induced Rat Heart Injury. in 7th Meeting of the European section and 8th Meeting of the North American section of the International academy of cardiovascular sciences (IACS); 2021 Sep 20-23; Banja Luka, Bosnia and Herzegovina. 2021;:57.
https://hdl.handle.net/21.15107/rcub_ibiss_4896 .

Lakić, Iva, Đurašević, Siniša, Ružičić, Aleksandra, Tosti, Tomislav, Đurović, Saša, Glumac, Sofija, Pavlović, Slađan, Borković Mitić, Slavica, Grigorov, Ilijana, Stanković, Sanja, Jasnić, Nebojša, Todorović, Zoran, Đorđević, Jelena, "The Effects of a Meldonium Pre-Treatment on the Sepsis-Induced Rat Heart Injury" in 7th Meeting of the European section and 8th Meeting of the North American section of the International academy of cardiovascular sciences (IACS); 2021 Sep 20-23; Banja Luka, Bosnia and Herzegovina (2021):57,
https://hdl.handle.net/21.15107/rcub_ibiss_4896 .

TY  - JOUR
AU  - Pavlović, Slađan
AU  - Krizmanić, Imre
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Mitić, Bojan
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4054
AB  - We investigated the activities of superoxide dismutase (SOD), catalase (CAT), glutathione
peroxidase (GSH-Px), glutathione reductase (GR) and the phase II biotransformation enzyme glutathione
S-transferase (GST) in the whole body of fire salamander larvae (Salamandra salamandra) from two
localities on Mt. Avala (AVS and ABP) and one locality on Mt. Vršački Breg (VSB), Serbia. We also
determined the total glutathione (GSH) and sulfhydryl group (SH) contents, as well as the concentrations of
manganese (Mn), copper (Cu), zinc (Zn), selenium (Se), arsenic (As), cadmium (Cd), lead (Pb) and uranium
(U). The obtained results show that animals from VSB had significantly lower weights and lengths than
animals from AVS and ABP. The activities of all investigated enzymes were significantly higher, while the
SH content was significantly lower in animals from VSB compared to those from AVS and ABP. No
correlations between trace-element concentrations in water and animal tissue were observed. We concluded
that the obtained results were more likely a consequence of the combination of developmental differences
and the effects of different habitat conditions, environmental and anthropogenic influences than of
concentrations of trace elements in the water alone.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia)
IS  - 4
VL  - 72
DO  - 10.2298/ABS200825043P
SP  - 491
EP  - 501
ER  - 

@article{
author = "Pavlović, Slađan and Krizmanić, Imre and Borković Mitić, Slavica and Stojsavljević, Aleksandar and Mitić, Bojan",
year = "2020",
abstract = "We investigated the activities of superoxide dismutase (SOD), catalase (CAT), glutathione
peroxidase (GSH-Px), glutathione reductase (GR) and the phase II biotransformation enzyme glutathione
S-transferase (GST) in the whole body of fire salamander larvae (Salamandra salamandra) from two
localities on Mt. Avala (AVS and ABP) and one locality on Mt. Vršački Breg (VSB), Serbia. We also
determined the total glutathione (GSH) and sulfhydryl group (SH) contents, as well as the concentrations of
manganese (Mn), copper (Cu), zinc (Zn), selenium (Se), arsenic (As), cadmium (Cd), lead (Pb) and uranium
(U). The obtained results show that animals from VSB had significantly lower weights and lengths than
animals from AVS and ABP. The activities of all investigated enzymes were significantly higher, while the
SH content was significantly lower in animals from VSB compared to those from AVS and ABP. No
correlations between trace-element concentrations in water and animal tissue were observed. We concluded
that the obtained results were more likely a consequence of the combination of developmental differences
and the effects of different habitat conditions, environmental and anthropogenic influences than of
concentrations of trace elements in the water alone.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia)",
number = "4",
volume = "72",
doi = "10.2298/ABS200825043P",
pages = "491-501"
}

Pavlović, S., Krizmanić, I., Borković Mitić, S., Stojsavljević, A.,& Mitić, B.. (2020). A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia). in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 72(4), 491-501.
https://doi.org/10.2298/ABS200825043P

Pavlović S, Krizmanić I, Borković Mitić S, Stojsavljević A, Mitić B. A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia). in Archives of Biological Sciences. 2020;72(4):491-501.
doi:10.2298/ABS200825043P .

Pavlović, Slađan, Krizmanić, Imre, Borković Mitić, Slavica, Stojsavljević, Aleksandar, Mitić, Bojan, "A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia)" in Archives of Biological Sciences, 72, no. 4 (2020):491-501,
https://doi.org/10.2298/ABS200825043P . .

TY  - JOUR
AU  - Vujotić, Ljiljana
AU  - Matić, Siniša
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Mutić, Jelena
AU  - Baščarević, Vladimir
AU  - Joković, Miloš
AU  - Pavlović, Slađan
PY  - 2020
UR  - https://www.termedia.pl/Association-between-oxidative-stress-biomarkers-and-concentrations-of-some-metal-ions-in-the-blood-of-patients-with-brain-tumors-and-hydrocephalus,19,37458,1,1.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3459
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3713
AB  - IntroductionAny substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.Material and methodsOur study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.ResultsThe higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.ConclusionsThere are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.
PB  - Termedia & Banach
T2  - Archives of Medical Science
T1  - Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus
IS  - 4
VL  - 16
DO  - 10.5114/aoms.2019.87409
SP  - 811
EP  - 819
ER  - 

@article{
author = "Vujotić, Ljiljana and Matić, Siniša and Borković Mitić, Slavica and Stojsavljević, Aleksandar and Mutić, Jelena and Baščarević, Vladimir and Joković, Miloš and Pavlović, Slađan",
year = "2020",
abstract = "IntroductionAny substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.Material and methodsOur study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.ResultsThe higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.ConclusionsThere are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.",
publisher = "Termedia & Banach",
journal = "Archives of Medical Science",
title = "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus",
number = "4",
volume = "16",
doi = "10.5114/aoms.2019.87409",
pages = "811-819"
}

Vujotić, L., Matić, S., Borković Mitić, S., Stojsavljević, A., Mutić, J., Baščarević, V., Joković, M.,& Pavlović, S.. (2020). Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science
Termedia & Banach., 16(4), 811-819.
https://doi.org/10.5114/aoms.2019.87409

Vujotić L, Matić S, Borković Mitić S, Stojsavljević A, Mutić J, Baščarević V, Joković M, Pavlović S. Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science. 2020;16(4):811-819.
doi:10.5114/aoms.2019.87409 .

Vujotić, Ljiljana, Matić, Siniša, Borković Mitić, Slavica, Stojsavljević, Aleksandar, Mutić, Jelena, Baščarević, Vladimir, Joković, Miloš, Pavlović, Slađan, "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus" in Archives of Medical Science, 16, no. 4 (2020):811-819,
https://doi.org/10.5114/aoms.2019.87409 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Jagodić, Jovana
AU  - Vujotić, Ljiljana
AU  - Borković Mitić, Slavica
AU  - Rašić-Milutinović, Zorica
AU  - Jovanović, Dragana
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2020
UR  - http://link.springer.com/10.1007/s11356-019-06936-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3530
AB  - This study was aimed to determine reference values (RVs) for the manganese (Mn), copper (Cu), zinc (Zn), and selenium (Se) in the whole blood (B) and serum (S) samples of the Serbian population. Blood specimens were collected from healthy persons (n = 295; women/men ratio = 149/146; mean age: 42 ± 2 years). The RVs were calculated as lower limit (LL) and upper limit (UL) of the 95% confidence interval (CI) and were expressed as percentiles (P) in the range from P2.5 to P97.5. The influences of sex, age, and smoking habits on element profiles were considered. It was found that the contents of B-Cu and S-Cu were higher in women, while the contents of B-Zn and S-Zn were higher in men. Both trace elements were significantly increased in a group of persons above 40 when compared to a younger persons (≤ 40 years). According to smoking habits, increased content was found only for S-Mn in the nonsmoker's group (p < 0.05). Comparing our results to the results reported in other population groups worldwide, the Serbian population had significantly reduced content of Se in both types of samples. This finding could highlight the deficiency of Se in the investigated Serbian population and could contribute to the better understanding of the molecular basis for the increased incidence of thyroid and other diseases in which selenium plays a key role.
T2  - Environmental Science and Pollution Research
T1  - Reference values for trace essential elements in the whole blood and serum samples of the adult Serbian population: significance of selenium deficiency.
IS  - 2
VL  - 27
DO  - 10.1007/s11356-019-06936-8
SP  - 1397
EP  - 1405
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Jagodić, Jovana and Vujotić, Ljiljana and Borković Mitić, Slavica and Rašić-Milutinović, Zorica and Jovanović, Dragana and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2020",
abstract = "This study was aimed to determine reference values (RVs) for the manganese (Mn), copper (Cu), zinc (Zn), and selenium (Se) in the whole blood (B) and serum (S) samples of the Serbian population. Blood specimens were collected from healthy persons (n = 295; women/men ratio = 149/146; mean age: 42 ± 2 years). The RVs were calculated as lower limit (LL) and upper limit (UL) of the 95% confidence interval (CI) and were expressed as percentiles (P) in the range from P2.5 to P97.5. The influences of sex, age, and smoking habits on element profiles were considered. It was found that the contents of B-Cu and S-Cu were higher in women, while the contents of B-Zn and S-Zn were higher in men. Both trace elements were significantly increased in a group of persons above 40 when compared to a younger persons (≤ 40 years). According to smoking habits, increased content was found only for S-Mn in the nonsmoker's group (p < 0.05). Comparing our results to the results reported in other population groups worldwide, the Serbian population had significantly reduced content of Se in both types of samples. This finding could highlight the deficiency of Se in the investigated Serbian population and could contribute to the better understanding of the molecular basis for the increased incidence of thyroid and other diseases in which selenium plays a key role.",
journal = "Environmental Science and Pollution Research",
title = "Reference values for trace essential elements in the whole blood and serum samples of the adult Serbian population: significance of selenium deficiency.",
number = "2",
volume = "27",
doi = "10.1007/s11356-019-06936-8",
pages = "1397-1405"
}

Stojsavljević, A., Jagodić, J., Vujotić, L., Borković Mitić, S., Rašić-Milutinović, Z., Jovanović, D., Gavrović-Jankulović, M.,& Manojlović, D.. (2020). Reference values for trace essential elements in the whole blood and serum samples of the adult Serbian population: significance of selenium deficiency.. in Environmental Science and Pollution Research, 27(2), 1397-1405.
https://doi.org/10.1007/s11356-019-06936-8

Stojsavljević A, Jagodić J, Vujotić L, Borković Mitić S, Rašić-Milutinović Z, Jovanović D, Gavrović-Jankulović M, Manojlović D. Reference values for trace essential elements in the whole blood and serum samples of the adult Serbian population: significance of selenium deficiency.. in Environmental Science and Pollution Research. 2020;27(2):1397-1405.
doi:10.1007/s11356-019-06936-8 .

Stojsavljević, Aleksandar, Jagodić, Jovana, Vujotić, Ljiljana, Borković Mitić, Slavica, Rašić-Milutinović, Zorica, Jovanović, Dragana, Gavrović-Jankulović, Marija, Manojlović, Dragan, "Reference values for trace essential elements in the whole blood and serum samples of the adult Serbian population: significance of selenium deficiency." in Environmental Science and Pollution Research, 27, no. 2 (2020):1397-1405,
https://doi.org/10.1007/s11356-019-06936-8 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Vujotić, Ljiljana
AU  - Rovčanin, Branislav
AU  - Borković Mitić, Slavica
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3624
AB  - The pathogenesis of malignant brain tumors (MBTs) should be better understood due to the evident association between prolonged exposure to metals and increased risk of MBTs. The present research aimed to find trace metals that could contribute to the pathogenesis of MBTs. Essential trace elements (Mn, Co, Zn, Cu, Se) and relevant toxic metals (Al, Ni, As, Sr, Cd, Ce, Pt, Pb, U) in the serum, cell fraction (CF), cerebrospinal fluid (CSF) and cancerous tissue (CT) samples of MBT patients were analyzed. The results were compared with sex- and age-matched control groups. For the first time, this research showed that elemental profiles of serum, CF, CSF and CT samples in MBT patients were significantly altered compared to the appropriate controls, as well as that higher contents of trace elements (particularly Mn, Se, and Pb) could be involved in the pathogenesis of MBTs. However, the most noticeable change found was the elevated U content, indicating its considerable role as a major cerebral discriminator of the presence/absence of MBTs. The U/Se ratio could be considered as an appropriate blood marker in diagnostic MBT evaluation. The reported results could contribute to better understanding of the poorly understood pathogenesis of MBTs. Furthermore, the reported results could highlight a molecular basis for the pathophysiological changes caused by the hazardous effects of trace metals on brain homeostasis.
T2  - Scientific Reports
T1  - Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-60774-0
SP  - 3816
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Vujotić, Ljiljana and Rovčanin, Branislav and Borković Mitić, Slavica and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2020",
abstract = "The pathogenesis of malignant brain tumors (MBTs) should be better understood due to the evident association between prolonged exposure to metals and increased risk of MBTs. The present research aimed to find trace metals that could contribute to the pathogenesis of MBTs. Essential trace elements (Mn, Co, Zn, Cu, Se) and relevant toxic metals (Al, Ni, As, Sr, Cd, Ce, Pt, Pb, U) in the serum, cell fraction (CF), cerebrospinal fluid (CSF) and cancerous tissue (CT) samples of MBT patients were analyzed. The results were compared with sex- and age-matched control groups. For the first time, this research showed that elemental profiles of serum, CF, CSF and CT samples in MBT patients were significantly altered compared to the appropriate controls, as well as that higher contents of trace elements (particularly Mn, Se, and Pb) could be involved in the pathogenesis of MBTs. However, the most noticeable change found was the elevated U content, indicating its considerable role as a major cerebral discriminator of the presence/absence of MBTs. The U/Se ratio could be considered as an appropriate blood marker in diagnostic MBT evaluation. The reported results could contribute to better understanding of the poorly understood pathogenesis of MBTs. Furthermore, the reported results could highlight a molecular basis for the pathophysiological changes caused by the hazardous effects of trace metals on brain homeostasis.",
journal = "Scientific Reports",
title = "Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-60774-0",
pages = "3816"
}

Stojsavljević, A., Vujotić, L., Rovčanin, B., Borković Mitić, S., Gavrović-Jankulović, M.,& Manojlović, D.. (2020). Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors. in Scientific Reports, 10(1), 3816.
https://doi.org/10.1038/s41598-020-60774-0

Stojsavljević A, Vujotić L, Rovčanin B, Borković Mitić S, Gavrović-Jankulović M, Manojlović D. Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors. in Scientific Reports. 2020;10(1):3816.
doi:10.1038/s41598-020-60774-0 .

Stojsavljević, Aleksandar, Vujotić, Ljiljana, Rovčanin, Branislav, Borković Mitić, Slavica, Gavrović-Jankulović, Marija, Manojlović, Dragan, "Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors" in Scientific Reports, 10, no. 1 (2020):3816,
https://doi.org/10.1038/s41598-020-60774-0 . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Stojković, Maja
AU  - Bogdanović, Ljiljana
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Jasnić, Nebojša
AU  - Tosti, Tomislav
AU  - Đurović, Saša
AU  - Đorđević, Jelena
AU  - Todorović, Zoran
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3519
AB  - Acute renal ischemia/reperfusion (I/R) injury is a clinical condition that is challenging to
treat. Meldonium is an anti-ischemic agent that shifts energy production from fatty acid oxidation
to less oxygen-consuming glycolysis. Thus, in this study we investigated the effects of a four-week
meldonium pre-treatment (300 mg/kg b.m./day) on acute renal I/R in male rats (Wistar strain). Our
results showed that meldonium decreased animal body mass gain, food and water intake, and
carnitine, glucose, and lactic acid kidney content. In kidneys of animals subjected to I/R, meldonium
increased phosphorylation of mitogen-activated protein kinase p38 and protein kinase B, and
increased the expression of nuclear factor erythroid 2-related factor 2 and haeme oxygenase 1,
causing manganese superoxide dismutase expression and activity to increase, as well as lipid
peroxidation, cooper-zinc superoxide dismutase, glutathione peroxidase, and glutathione reductase
activities to decrease. By decreasing the kidney Bax/Bcl2 expression ratio and kidney and serum
high mobility group box 1 protein content, meldonium reduced apoptotic and necrotic events in
I/R, as confirmed by kidney histology. Meldonium increased adrenal noradrenaline content and
serum, adrenal, hepatic, and renal ascorbic/dehydroascorbic acid ratio, which caused complex
changes in renal lipidomics. Taken together, our results have confirmed that meldonium pretreatment protects against I/R-induced oxidative stress and apoptosis/necrosis.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - The effects of meldonium on the renal acute ischemia/reperfusion injury in rats
IS  - 22
VL  - 20
DO  - 10.3390/ijms20225747
SP  - 5747
ER  - 

@article{
author = "Đurašević, Siniša and Stojković, Maja and Bogdanović, Ljiljana and Pavlović, Slađan and Borković Mitić, Slavica and Grigorov, Ilijana and Bogojević, Desanka and Jasnić, Nebojša and Tosti, Tomislav and Đurović, Saša and Đorđević, Jelena and Todorović, Zoran",
year = "2019",
abstract = "Acute renal ischemia/reperfusion (I/R) injury is a clinical condition that is challenging to
treat. Meldonium is an anti-ischemic agent that shifts energy production from fatty acid oxidation
to less oxygen-consuming glycolysis. Thus, in this study we investigated the effects of a four-week
meldonium pre-treatment (300 mg/kg b.m./day) on acute renal I/R in male rats (Wistar strain). Our
results showed that meldonium decreased animal body mass gain, food and water intake, and
carnitine, glucose, and lactic acid kidney content. In kidneys of animals subjected to I/R, meldonium
increased phosphorylation of mitogen-activated protein kinase p38 and protein kinase B, and
increased the expression of nuclear factor erythroid 2-related factor 2 and haeme oxygenase 1,
causing manganese superoxide dismutase expression and activity to increase, as well as lipid
peroxidation, cooper-zinc superoxide dismutase, glutathione peroxidase, and glutathione reductase
activities to decrease. By decreasing the kidney Bax/Bcl2 expression ratio and kidney and serum
high mobility group box 1 protein content, meldonium reduced apoptotic and necrotic events in
I/R, as confirmed by kidney histology. Meldonium increased adrenal noradrenaline content and
serum, adrenal, hepatic, and renal ascorbic/dehydroascorbic acid ratio, which caused complex
changes in renal lipidomics. Taken together, our results have confirmed that meldonium pretreatment protects against I/R-induced oxidative stress and apoptosis/necrosis.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "The effects of meldonium on the renal acute ischemia/reperfusion injury in rats",
number = "22",
volume = "20",
doi = "10.3390/ijms20225747",
pages = "5747"
}

Đurašević, S., Stojković, M., Bogdanović, L., Pavlović, S., Borković Mitić, S., Grigorov, I., Bogojević, D., Jasnić, N., Tosti, T., Đurović, S., Đorđević, J.,& Todorović, Z.. (2019). The effects of meldonium on the renal acute ischemia/reperfusion injury in rats. in International Journal of Molecular Sciences
MDPI., 20(22), 5747.
https://doi.org/10.3390/ijms20225747

Đurašević S, Stojković M, Bogdanović L, Pavlović S, Borković Mitić S, Grigorov I, Bogojević D, Jasnić N, Tosti T, Đurović S, Đorđević J, Todorović Z. The effects of meldonium on the renal acute ischemia/reperfusion injury in rats. in International Journal of Molecular Sciences. 2019;20(22):5747.
doi:10.3390/ijms20225747 .

Đurašević, Siniša, Stojković, Maja, Bogdanović, Ljiljana, Pavlović, Slađan, Borković Mitić, Slavica, Grigorov, Ilijana, Bogojević, Desanka, Jasnić, Nebojša, Tosti, Tomislav, Đurović, Saša, Đorđević, Jelena, Todorović, Zoran, "The effects of meldonium on the renal acute ischemia/reperfusion injury in rats" in International Journal of Molecular Sciences, 20, no. 22 (2019):5747,
https://doi.org/10.3390/ijms20225747 . .

TY  - JOUR
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Anđelković, Marko
AU  - Tomović, Ljiljana
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S1532045619300924?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3351
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3365
AB  - In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?
VL  - 223
DO  - 10.1016/J.CBPC.2019.05.010
SP  - 26
EP  - 34
ER  - 

@article{
author = "Gavrić, Jelena and Despotović, Svetlana and Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Anđelković, Marko and Tomović, Ljiljana and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2019",
abstract = "In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?",
volume = "223",
doi = "10.1016/J.CBPC.2019.05.010",
pages = "26-34"
}

Gavrić, J., Despotović, S., Prokić, M., Gavrilović, B., Radovanović, T., Anđelković, M., Tomović, L., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2019). Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 223, 26-34.
https://doi.org/10.1016/J.CBPC.2019.05.010

Gavrić J, Despotović S, Prokić M, Gavrilović B, Radovanović T, Anđelković M, Tomović L, Borković Mitić S, Pavlović S, Saičić Z. Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2019;223:26-34.
doi:10.1016/J.CBPC.2019.05.010 .

Gavrić, Jelena, Despotović, Svetlana, Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Anđelković, Marko, Tomović, Ljiljana, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 223 (2019):26-34,
https://doi.org/10.1016/J.CBPC.2019.05.010 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa
AU  - Borković Mitić, Slavica
AU  - Paunović, Ivan
AU  - Diklić, Aleksandar
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2019
UR  - http://link.springer.com/10.1007/s12403-019-00309-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3359
AB  - The background level of lead (Pb) in drinking water in Serbia represents a significant health issue. The aim of study was to examine the content of significant toxic and essential trace metals (Mn, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U) in the thyroid tissues with diagnosed colloid goiter (CG). Different variables were investigated (sex, age, smoking habits, goiter size, and previous history of any thyroid disease). The study was designed to compare the goiter tissues (GTs) to sex- and age-matched healthy- and malignant-thyroid tissues (HTTs and MTTs, respectively). All trace metals were quantified by inductively coupled plasma mass spectrometry (ICP-MS). The reduced content of essential elements (Mn and Se) and increased content of toxic metals (Pb, Th, and U) were found in GTs compared with HTTs. The increased content of the same toxic metals separated GTs from MTTs, as well as reduced content of Mn and Cd. Our results indicated that Pb acts as the main goitrogen, which could highlight its role in the unknown etiology of CG disease. Pb/Se ratio could be considered as a relevant parameter for the tissue separation of GTs from HTTs and/or MTTs. This research also provides novel data on the effects of metal pollutants on thyroid health hazards.
T2  - Exposure and Health
T1  - Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease
DO  - 10.1007/s12403-019-00309-9
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa and Borković Mitić, Slavica and Paunović, Ivan and Diklić, Aleksandar and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2019",
abstract = "The background level of lead (Pb) in drinking water in Serbia represents a significant health issue. The aim of study was to examine the content of significant toxic and essential trace metals (Mn, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U) in the thyroid tissues with diagnosed colloid goiter (CG). Different variables were investigated (sex, age, smoking habits, goiter size, and previous history of any thyroid disease). The study was designed to compare the goiter tissues (GTs) to sex- and age-matched healthy- and malignant-thyroid tissues (HTTs and MTTs, respectively). All trace metals were quantified by inductively coupled plasma mass spectrometry (ICP-MS). The reduced content of essential elements (Mn and Se) and increased content of toxic metals (Pb, Th, and U) were found in GTs compared with HTTs. The increased content of the same toxic metals separated GTs from MTTs, as well as reduced content of Mn and Cd. Our results indicated that Pb acts as the main goitrogen, which could highlight its role in the unknown etiology of CG disease. Pb/Se ratio could be considered as a relevant parameter for the tissue separation of GTs from HTTs and/or MTTs. This research also provides novel data on the effects of metal pollutants on thyroid health hazards.",
journal = "Exposure and Health",
title = "Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease",
doi = "10.1007/s12403-019-00309-9"
}

Stojsavljević, A., Rovčanin, B., Krstić, Đ., Borković Mitić, S., Paunović, I., Diklić, A., Gavrović-Jankulović, M.,& Manojlović, D.. (2019). Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease. in Exposure and Health.
https://doi.org/10.1007/s12403-019-00309-9

Stojsavljević A, Rovčanin B, Krstić Đ, Borković Mitić S, Paunović I, Diklić A, Gavrović-Jankulović M, Manojlović D. Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease. in Exposure and Health. 2019;.
doi:10.1007/s12403-019-00309-9 .

Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa, Borković Mitić, Slavica, Paunović, Ivan, Diklić, Aleksandar, Gavrović-Jankulović, Marija, Manojlović, Dragan, "Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease" in Exposure and Health (2019),
https://doi.org/10.1007/s12403-019-00309-9 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa
AU  - Jagodić, Jovana
AU  - Borković Mitić, Slavica
AU  - Paunović, Ivan
AU  - Živaljević, Vladan
AU  - Mitić, Bojan
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0946672X19300070?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3435
AB  - BACKGROUND The etiology of papillary thyroid carcinoma (PTC) is unknown and some literature data support the hypothesis that heavy metals, as endocrine disrupters, could play a major role in the pathogenesis of thyroid cancer. This study aimed to estimate the content of selected toxic and essential trace metals (Mn, Co, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U), as well as the selected ratio’s (Cu/Zn and Cd/Se) in the malignant thyroid tissues according to sex, age, smoking habits, familial history of any thyroid disease, pathohistological (PH) types of PTC, tumor size, the existence of a thyroid capsular invasion, intrathyroid tumor dissemination, retrosternal thyroid growth, and TNM progress of PTC. METHODS The study included 66 patients with PTC (women/men ratio = 46/20, mean age: 54 ± 14 years). A comparative analysis was made by collecting the healthy thyroid tissues (HTTs) of the same patients, making the total number of samples 132. All trace metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). RESULTS Metals that significantly separated papillary thyroid tissues (PTTs) from the HTTs were Cd, U and Se (p < 0.05). The obtained negative correlation between Cd and Se in the PTTs could explain extrusion of essential Se caused by increased content of Cd. Only Cd had an influence on the retrosternal thyroid growth, while the essential metals (Mn, Co, and Zn) had an influence on thyroid capsular invasion. CONCLUSION It was found that Cd act as the main endocrine disrupter, which could highlight its role in the etiology of PTC. Considering that the Cd/Se ratio significantly separated two studied groups and had an influence on the retrosternal thyroid growth, its altered content could contribute to the better understanding of the molecular basis for pathophysiological changes in the PTC.
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology
VL  - 55
DO  - 10.1016/J.JTEMB.2019.06.009
SP  - 190
EP  - 195
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa and Jagodić, Jovana and Borković Mitić, Slavica and Paunović, Ivan and Živaljević, Vladan and Mitić, Bojan and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2019",
abstract = "BACKGROUND The etiology of papillary thyroid carcinoma (PTC) is unknown and some literature data support the hypothesis that heavy metals, as endocrine disrupters, could play a major role in the pathogenesis of thyroid cancer. This study aimed to estimate the content of selected toxic and essential trace metals (Mn, Co, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U), as well as the selected ratio’s (Cu/Zn and Cd/Se) in the malignant thyroid tissues according to sex, age, smoking habits, familial history of any thyroid disease, pathohistological (PH) types of PTC, tumor size, the existence of a thyroid capsular invasion, intrathyroid tumor dissemination, retrosternal thyroid growth, and TNM progress of PTC. METHODS The study included 66 patients with PTC (women/men ratio = 46/20, mean age: 54 ± 14 years). A comparative analysis was made by collecting the healthy thyroid tissues (HTTs) of the same patients, making the total number of samples 132. All trace metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). RESULTS Metals that significantly separated papillary thyroid tissues (PTTs) from the HTTs were Cd, U and Se (p < 0.05). The obtained negative correlation between Cd and Se in the PTTs could explain extrusion of essential Se caused by increased content of Cd. Only Cd had an influence on the retrosternal thyroid growth, while the essential metals (Mn, Co, and Zn) had an influence on thyroid capsular invasion. CONCLUSION It was found that Cd act as the main endocrine disrupter, which could highlight its role in the etiology of PTC. Considering that the Cd/Se ratio significantly separated two studied groups and had an influence on the retrosternal thyroid growth, its altered content could contribute to the better understanding of the molecular basis for pathophysiological changes in the PTC.",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology",
volume = "55",
doi = "10.1016/J.JTEMB.2019.06.009",
pages = "190-195"
}

Stojsavljević, A., Rovčanin, B., Krstić, Đ., Jagodić, J., Borković Mitić, S., Paunović, I., Živaljević, V., Mitić, B., Gavrović-Jankulović, M.,& Manojlović, D.. (2019). Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology. in Journal of Trace Elements in Medicine and Biology, 55, 190-195.
https://doi.org/10.1016/J.JTEMB.2019.06.009

Stojsavljević A, Rovčanin B, Krstić Đ, Jagodić J, Borković Mitić S, Paunović I, Živaljević V, Mitić B, Gavrović-Jankulović M, Manojlović D. Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology. in Journal of Trace Elements in Medicine and Biology. 2019;55:190-195.
doi:10.1016/J.JTEMB.2019.06.009 .

Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa, Jagodić, Jovana, Borković Mitić, Slavica, Paunović, Ivan, Živaljević, Vladan, Mitić, Bojan, Gavrović-Jankulović, Marija, Manojlović, Dragan, "Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology" in Journal of Trace Elements in Medicine and Biology, 55 (2019):190-195,
https://doi.org/10.1016/J.JTEMB.2019.06.009 . .

TY  - JOUR
AU  - Vujotić, Ljiljana
AU  - Matić, Siniša
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Mutić, Jelena
AU  - Baščarević, Vladimir
AU  - Joković, Miloš
AU  - Pavlović, Slađan
PY  - 2019
UR  - https://www.termedia.pl/Association-between-oxidative-stress-biomarkers-and-concentrations-of-some-metal-ions-in-the-blood-of-patients-with-brain-tumors-and-hydrocephalus,19,37458,1,1.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3459
AB  - Introduction
Any substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.

Material and methods
Our study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.

Results
The higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.

Conclusions
There are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.
PB  - Termedia & Banach
T2  - Archives of Medical Science
T1  - Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus
DO  - 10.5114/aoms.2019.87409
ER  - 

@article{
author = "Vujotić, Ljiljana and Matić, Siniša and Borković Mitić, Slavica and Stojsavljević, Aleksandar and Mutić, Jelena and Baščarević, Vladimir and Joković, Miloš and Pavlović, Slađan",
year = "2019",
abstract = "Introduction
Any substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.

Material and methods
Our study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.

Results
The higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.

Conclusions
There are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.",
publisher = "Termedia & Banach",
journal = "Archives of Medical Science",
title = "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus",
doi = "10.5114/aoms.2019.87409"
}

Vujotić, L., Matić, S., Borković Mitić, S., Stojsavljević, A., Mutić, J., Baščarević, V., Joković, M.,& Pavlović, S.. (2019). Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science
Termedia & Banach..
https://doi.org/10.5114/aoms.2019.87409

Vujotić L, Matić S, Borković Mitić S, Stojsavljević A, Mutić J, Baščarević V, Joković M, Pavlović S. Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science. 2019;.
doi:10.5114/aoms.2019.87409 .

Vujotić, Ljiljana, Matić, Siniša, Borković Mitić, Slavica, Stojsavljević, Aleksandar, Mutić, Jelena, Baščarević, Vladimir, Joković, Miloš, Pavlović, Slađan, "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus" in Archives of Medical Science (2019),
https://doi.org/10.5114/aoms.2019.87409 . .

TY  - JOUR
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Prokić, Marko
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Anđelković, Marko
AU  - Tomović, Ljiljana
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S1532045619300924?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3351
AB  - In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?
DO  - 10.1016/J.CBPC.2019.05.010
ER  - 

@article{
author = "Gavrić, Jelena and Despotović, Svetlana and Prokić, Marko and Gavrilović, Branka and Radovanović, Tijana and Anđelković, Marko and Tomović, Ljiljana and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2019",
abstract = "In this study we examined possible differences in heavy metal accumulation and oxidative stress parameters in the liver and muscle of two semi-aquatic snakes: grass snake (Natrix natrix) and dice snake (N. tessellata), that inhabit the same environment but differ in prey diversity. The obtained results revealed some interspecies, inter-tissue, prey-snake and prey-prey differences in heavy metal concentrations. Grass snakes pray contained significantly higher concentrations of Al, Cr and Fe as compared to food of dice snakes. Both investigated snakes accumulated generally lower concentrations of metals than their prey, indicating that they are not at risk of contaminant biomagnification. A significant interspecies difference in accumulation was observed only for Cu and Mn concentrations. On the other hand, analysis of oxidative stress biomarkers showed clear differences between the investigated snake species and the two investigated tissues. The liver of grass snake had increased superoxide dismutase, glutathione reductase and glutathione-S-transferase activities in comparison to dice snake. In muscle, a reverse trend was observed for the activities of these three enzymes, as well as for glutathione peroxidase activity. The higher number of significant correlations observed between oxidative stress biomarkers and heavy metal concentrations in grass snake points to upregulation of the antioxidative system (AOS), which resulted in a lower TBARS concentration. Results show that while the investigated snake species did not differ significantly in the accumulated metals, their defense mechanisms were different. This reveals the complexity of the AOS and points to the cooperation of different AOS components in individuals from natural populations.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?",
doi = "10.1016/J.CBPC.2019.05.010"
}

Gavrić, J., Despotović, S., Prokić, M., Gavrilović, B., Radovanović, T., Anđelković, M., Tomović, L., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2019). Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology.
https://doi.org/10.1016/J.CBPC.2019.05.010

Gavrić J, Despotović S, Prokić M, Gavrilović B, Radovanović T, Anđelković M, Tomović L, Borković Mitić S, Pavlović S, Saičić Z. Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2019;.
doi:10.1016/J.CBPC.2019.05.010 .

Gavrić, Jelena, Despotović, Svetlana, Prokić, Marko, Gavrilović, Branka, Radovanović, Tijana, Anđelković, Marko, Tomović, Ljiljana, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Do different diets affect oxidative stress biomarkers and metal bioaccumulation in two snake species?" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology (2019),
https://doi.org/10.1016/J.CBPC.2019.05.010 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa
AU  - Borković Mitić, Slavica
AU  - Paunović, Ivan
AU  - Kodranov, Igor
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0147651319308103?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3450
AB  - Evaluation of trace metals at level of solid tissue can provide better information than blood or urine and, therefore, could highlight the role of metals in the etiology of organ-specific disease. The current study aimed to establish the baseline content of four essential (Mn, Cu, Zn, Se) and four toxic metals (As, Cd, Pb, U) in the healthy thyroid tissues (HTTs) by considering sex, age and smoking habits. A further aim was to examine whether differences in the content of metals exist in regard to the thyroid diseases, such as benign tumor (BT), Hashimoto's thyroiditis (HT), multinodular goiter (MNG) and thyroid cancer (TC). A total number of investigated tissue samples were 423. All metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). It was found that the content of Cu and U was higher in HTTs of women, while the content of Zn was higher in HTTs of men. Increased content of Zn and decreased content of U was found in the group of HTTs above 50 years compared to a younger group (<50 years). Increased content of Cd, Pb and U distinguish smokers from the non-smokers. In comparison with other population groups worldwide, investigated Serbian population had up to 15 times reduced content of Se. Despite the difference in metal's profile according to biological variables, this study also demonstrated, for the first time, that each thyroid disease has its unique metal's profile. The most altered metal's content was found in tissues with HT. Contrarily, the greatest similarity in metal's content with HTTs was found in BT tissues. Based on the increased content, metal's that dominantly discriminated HTTs from the HT, MNG and TC was As, Pb and Cd, respectively. Reported results could highlight the role of toxic and essential trace metals in the not very well clarified etiology of thyroid diseases and, moreover, could provide a molecular basis for pathophysiological changes of metal's hazardous effects on thyroid health at the tissue level.
T2  - Ecotoxicology and Environmental Safety
T1  - Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases.
VL  - 183
DO  - 10.1016/j.ecoenv.2019.109479
SP  - 109479
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa and Borković Mitić, Slavica and Paunović, Ivan and Kodranov, Igor and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2019",
abstract = "Evaluation of trace metals at level of solid tissue can provide better information than blood or urine and, therefore, could highlight the role of metals in the etiology of organ-specific disease. The current study aimed to establish the baseline content of four essential (Mn, Cu, Zn, Se) and four toxic metals (As, Cd, Pb, U) in the healthy thyroid tissues (HTTs) by considering sex, age and smoking habits. A further aim was to examine whether differences in the content of metals exist in regard to the thyroid diseases, such as benign tumor (BT), Hashimoto's thyroiditis (HT), multinodular goiter (MNG) and thyroid cancer (TC). A total number of investigated tissue samples were 423. All metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). It was found that the content of Cu and U was higher in HTTs of women, while the content of Zn was higher in HTTs of men. Increased content of Zn and decreased content of U was found in the group of HTTs above 50 years compared to a younger group (<50 years). Increased content of Cd, Pb and U distinguish smokers from the non-smokers. In comparison with other population groups worldwide, investigated Serbian population had up to 15 times reduced content of Se. Despite the difference in metal's profile according to biological variables, this study also demonstrated, for the first time, that each thyroid disease has its unique metal's profile. The most altered metal's content was found in tissues with HT. Contrarily, the greatest similarity in metal's content with HTTs was found in BT tissues. Based on the increased content, metal's that dominantly discriminated HTTs from the HT, MNG and TC was As, Pb and Cd, respectively. Reported results could highlight the role of toxic and essential trace metals in the not very well clarified etiology of thyroid diseases and, moreover, could provide a molecular basis for pathophysiological changes of metal's hazardous effects on thyroid health at the tissue level.",
journal = "Ecotoxicology and Environmental Safety",
title = "Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases.",
volume = "183",
doi = "10.1016/j.ecoenv.2019.109479",
pages = "109479"
}

Stojsavljević, A., Rovčanin, B., Krstić, Đ., Borković Mitić, S., Paunović, I., Kodranov, I., Gavrović-Jankulović, M.,& Manojlović, D.. (2019). Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases.. in Ecotoxicology and Environmental Safety, 183, 109479.
https://doi.org/10.1016/j.ecoenv.2019.109479

Stojsavljević A, Rovčanin B, Krstić Đ, Borković Mitić S, Paunović I, Kodranov I, Gavrović-Jankulović M, Manojlović D. Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases.. in Ecotoxicology and Environmental Safety. 2019;183:109479.
doi:10.1016/j.ecoenv.2019.109479 .

Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa, Borković Mitić, Slavica, Paunović, Ivan, Kodranov, Igor, Gavrović-Jankulović, Marija, Manojlović, Dragan, "Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases." in Ecotoxicology and Environmental Safety, 183 (2019):109479,
https://doi.org/10.1016/j.ecoenv.2019.109479 . .