TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Malbeck, Jiří
AU  - Dobrev, Petre I.
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Zahajská, Lenka
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3605
AB  - Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.
T2  - Journal of Plant Growth Regulation
T1  - Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization
VL  - 40
DO  - 10.1007/s00344-020-10080-w
SP  - 37
EP  - 47
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Malbeck, Jiří and Dobrev, Petre I. and Zdravković-Korać, Snežana and Simonović, Ana and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Zahajská, Lenka and Tadić, Vojin and Dragićević, Ivana",
year = "2021",
abstract = "Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.",
journal = "Journal of Plant Growth Regulation",
title = "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization",
volume = "40",
doi = "10.1007/s00344-020-10080-w",
pages = "37-47"
}

Raspor, M., Motyka, V., Ninković, S., Malbeck, J., Dobrev, P. I., Zdravković-Korać, S., Simonović, A., Ćosić, T., Cingel, A., Savić, J., Zahajská, L., Tadić, V.,& Dragićević, I.. (2021). Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation, 40, 37-47.
https://doi.org/10.1007/s00344-020-10080-w

Raspor M, Motyka V, Ninković S, Malbeck J, Dobrev PI, Zdravković-Korać S, Simonović A, Ćosić T, Cingel A, Savić J, Zahajská L, Tadić V, Dragićević I. Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation. 2021;40:37-47.
doi:10.1007/s00344-020-10080-w .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Malbeck, Jiří, Dobrev, Petre I., Zdravković-Korać, Snežana, Simonović, Ana, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Zahajská, Lenka, Tadić, Vojin, Dragićević, Ivana, "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization" in Journal of Plant Growth Regulation, 40 (2021):37-47,
https://doi.org/10.1007/s00344-020-10080-w . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Stanković, Slaviša
AU  - Uzelac, Branka
AU  - Tadić, Vojin
AU  - Trifunović-Momčilov, Milana
AU  - Lozo, Jelena
AU  - Marković, Marija
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/5/599
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3676
AB  - The main topic of this study is the bioremediation potential of the common duckweed, Lemna minor L., and selected rhizospheric bacterial strains in removing phenol from aqueous environments at extremely high initial phenol concentrations. To that end, fluorescence microscopy, MIC tests, biofilm formation, the phenol removal test (4-AAP method), the Salkowski essay, and studies of multiplication rates of sterile and inoculated duckweed in MS medium with phenol (200, 500, 750, and 1000 mg L−1) were conducted. Out of seven bacterial strains, six were identified as epiphytes or endophytes that efficiently removed phenol. The phenol removal experiment showed that the bacteria/duckweed system was more efficient during the first 24 h compared to the sterile duckweed control group. At the end of this experiment, almost 90% of the initial phenol concentration was removed by both groups, respectively. The bacteria stimulated the duckweed multiplication even at a high bacterial population density (>105 CFU mL−1) over a prolonged period of time (14 days). All bacterial strains were sensitive to all the applied antibiotics and formed biofilms in vitro. The dual bacteria/duckweed system, especially the one containing strain 43-Hafnia paralvei C32-106/3, Accession No. MF526939, had a number of characteristics that are advantageous in bioremediation, such as high phenol removal efficiency, biofilm formation, safety (antibiotic sensitivity), and stimulation of duckweed multiplication.
PB  - MDPI AG
T2  - Plants
T1  - Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations
IS  - 5
VL  - 9
DO  - 10.3390/plants9050599
SP  - 599
ER  - 

@article{
author = "Radulović, Olga and Stanković, Slaviša and Uzelac, Branka and Tadić, Vojin and Trifunović-Momčilov, Milana and Lozo, Jelena and Marković, Marija",
year = "2020",
abstract = "The main topic of this study is the bioremediation potential of the common duckweed, Lemna minor L., and selected rhizospheric bacterial strains in removing phenol from aqueous environments at extremely high initial phenol concentrations. To that end, fluorescence microscopy, MIC tests, biofilm formation, the phenol removal test (4-AAP method), the Salkowski essay, and studies of multiplication rates of sterile and inoculated duckweed in MS medium with phenol (200, 500, 750, and 1000 mg L−1) were conducted. Out of seven bacterial strains, six were identified as epiphytes or endophytes that efficiently removed phenol. The phenol removal experiment showed that the bacteria/duckweed system was more efficient during the first 24 h compared to the sterile duckweed control group. At the end of this experiment, almost 90% of the initial phenol concentration was removed by both groups, respectively. The bacteria stimulated the duckweed multiplication even at a high bacterial population density (>105 CFU mL−1) over a prolonged period of time (14 days). All bacterial strains were sensitive to all the applied antibiotics and formed biofilms in vitro. The dual bacteria/duckweed system, especially the one containing strain 43-Hafnia paralvei C32-106/3, Accession No. MF526939, had a number of characteristics that are advantageous in bioremediation, such as high phenol removal efficiency, biofilm formation, safety (antibiotic sensitivity), and stimulation of duckweed multiplication.",
publisher = "MDPI AG",
journal = "Plants",
title = "Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations",
number = "5",
volume = "9",
doi = "10.3390/plants9050599",
pages = "599"
}

Radulović, O., Stanković, S., Uzelac, B., Tadić, V., Trifunović-Momčilov, M., Lozo, J.,& Marković, M.. (2020). Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations. in Plants
MDPI AG., 9(5), 599.
https://doi.org/10.3390/plants9050599

Radulović O, Stanković S, Uzelac B, Tadić V, Trifunović-Momčilov M, Lozo J, Marković M. Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations. in Plants. 2020;9(5):599.
doi:10.3390/plants9050599 .

Radulović, Olga, Stanković, Slaviša, Uzelac, Branka, Tadić, Vojin, Trifunović-Momčilov, Milana, Lozo, Jelena, Marković, Marija, "Phenol Removal Capacity of the Common Duckweed (Lemna minor L.) and Six Phenol-Resistant Bacterial Strains From Its Rhizosphere: In Vitro Evaluation at High Phenol Concentrations" in Plants, 9, no. 5 (2020):599,
https://doi.org/10.3390/plants9050599 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Dobrev, Petre I.
AU  - Malbeck, Jiří
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2020
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7044434
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3623
AB  - A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.
T2  - Scientific Reports
T1  - Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-60412-9
SP  - 3437
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Dobrev, Petre I. and Malbeck, Jiří and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Tadić, Vojin and Dragićević, Ivana",
year = "2020",
abstract = "A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.",
journal = "Scientific Reports",
title = "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-60412-9",
pages = "3437"
}

Raspor, M., Motyka, V., Ninković, S., Dobrev, P. I., Malbeck, J., Ćosić, T., Cingel, A., Savić, J., Tadić, V.,& Dragićević, I.. (2020). Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports, 10(1), 3437.
https://doi.org/10.1038/s41598-020-60412-9

Raspor M, Motyka V, Ninković S, Dobrev PI, Malbeck J, Ćosić T, Cingel A, Savić J, Tadić V, Dragićević I. Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports. 2020;10(1):3437.
doi:10.1038/s41598-020-60412-9 .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Dobrev, Petre I., Malbeck, Jiří, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Tadić, Vojin, Dragićević, Ivana, "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics." in Scientific Reports, 10, no. 1 (2020):3437,
https://doi.org/10.1038/s41598-020-60412-9 . .

TY  - CONF
AU  - Tadić, Vojin
AU  - Radonjić, Vladimir
AU  - Jakovljević, Milan
AU  - Marković, Marija
AU  - Raspor, Martin
AU  - Milošević, Snežana
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6733
AB  - Životna sredina je decenijama unazad ozbiljno narušena antropogenim uticajima koji uzrokuju klimatske promene. S obzirom na to da se radi o globalnom problemu, mora se ozbiljno pristupiti njegovom rešavanju i smanjenju svih negativnih uticaja koji su uglavnom povezani sa povećanom emisijom gasova staklene bašte, a specijalno emisijom ugljen dioksida. Potencijalni veliki rpoblem JP EPS vezan je za uvođenje obaveze plaćanja takse za emisiju CO2 koja u ovom trenutku u svetu već iznosi 100 E/t. U ovom radu prvo će biti opisan potencijal visokoprinosnih biljnih vrsta za proizvodnju biomase, koja se može iskoristiti za proizvodnju energije u Srbiji. Ovakav koncept je veoma poželjan i primenljiv jer se procenjuje da 63% potencijala za proizvodnju električne energije Srbije leži baš u proizvodnji bimase. Smatra se da bi tzv. energetski zasadi visokoprinosnih biljnih vrsta mogli biti rešenje problema proizvodnje energije sa minimalnim negetivnim uticajem na životnu sredinnu. Biomasa koja bi se na taj način proizvodila mora biti kontinualna, sa konstantnim prinosima, opravdanih troškova transporta i sastavljena od neinvazivnih vrsta. Posebna pažnja posvećena je biljkama sa C4 metabolizmom, koje su adaptirane na smanjenu potrošnju vode, veliku produkciju bimase (300 t/ha/god) i rast na devastiranim površinama. Biće opisani potencijali sledećih biljnih vrsta koje se koriste za dobijanje viokoenergetske biomase paralelno kroz proces biološke rekultivacije: miskantus, sirak, slonovska trava, bambusi i paulovnija. Ove biljne vrste imaju kalorijske vrednosti koje su 2,67 puta veće od kalorijske vrednosti kolubarskog lignita, što ih čini idealnim kandidatima za proizvodnji ili dopunu pri proizvodnji energije. Dodatna prednost pri formiranju ovakvih zasada je biološka rekutivacija devastiranih zemljišta, koja podrazumeva ponovno uspostavljanje vegetacijskoh pokrivača, biološku remedijaciju, zaštitu od erozije i uspostavljanje vetrozaštitnih pojaseva. Pored navedenih prednosti ove biljke apsorbuju toksične gasove i ugljen dioksid iz atmosfere što dovodi do uspostavljanja povoljne mikroklime i CO2 neutralne proizvodnje energije, a zahvaljujući visokom sadržaju lignoceluloznih komponenti mogu se efikasno koristiti za proizvodnju tečnih goriva - bioetanola. Ukoliko bi ove biljke bile korišćene za biološku rekultivaciju zemljišta devstiranih površina JP EPS i bile efikasno upotrebljene kao supstituent uglja u proizvodnji energije, moglo bi se zameniti do 4,6% ukupne godišnje proizvodnje uglja i uštedeti preko 1,1 milijarde samo na emisiji CO2 i dodatno ostvariti ušteda na redukciji deponovanja pepela.
PB  - Beograd: Savez energetičara
C3  - Zbornik radova: 33. međunarodno savetovanje: Energetika 2017; 2017 Mar 28-31; Zlatibor, Serbia
T1  - Potencijal odabranih biljnih vrsta za formiranje energetskih zasada - koncept "biomasa kroz rekultivaciju"
IS  - 3-4
VL  - 19
SP  - 41
EP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6733
ER  - 

@conference{
author = "Tadić, Vojin and Radonjić, Vladimir and Jakovljević, Milan and Marković, Marija and Raspor, Martin and Milošević, Snežana",
year = "2017",
abstract = "Životna sredina je decenijama unazad ozbiljno narušena antropogenim uticajima koji uzrokuju klimatske promene. S obzirom na to da se radi o globalnom problemu, mora se ozbiljno pristupiti njegovom rešavanju i smanjenju svih negativnih uticaja koji su uglavnom povezani sa povećanom emisijom gasova staklene bašte, a specijalno emisijom ugljen dioksida. Potencijalni veliki rpoblem JP EPS vezan je za uvođenje obaveze plaćanja takse za emisiju CO2 koja u ovom trenutku u svetu već iznosi 100 E/t. U ovom radu prvo će biti opisan potencijal visokoprinosnih biljnih vrsta za proizvodnju biomase, koja se može iskoristiti za proizvodnju energije u Srbiji. Ovakav koncept je veoma poželjan i primenljiv jer se procenjuje da 63% potencijala za proizvodnju električne energije Srbije leži baš u proizvodnji bimase. Smatra se da bi tzv. energetski zasadi visokoprinosnih biljnih vrsta mogli biti rešenje problema proizvodnje energije sa minimalnim negetivnim uticajem na životnu sredinnu. Biomasa koja bi se na taj način proizvodila mora biti kontinualna, sa konstantnim prinosima, opravdanih troškova transporta i sastavljena od neinvazivnih vrsta. Posebna pažnja posvećena je biljkama sa C4 metabolizmom, koje su adaptirane na smanjenu potrošnju vode, veliku produkciju bimase (300 t/ha/god) i rast na devastiranim površinama. Biće opisani potencijali sledećih biljnih vrsta koje se koriste za dobijanje viokoenergetske biomase paralelno kroz proces biološke rekultivacije: miskantus, sirak, slonovska trava, bambusi i paulovnija. Ove biljne vrste imaju kalorijske vrednosti koje su 2,67 puta veće od kalorijske vrednosti kolubarskog lignita, što ih čini idealnim kandidatima za proizvodnji ili dopunu pri proizvodnji energije. Dodatna prednost pri formiranju ovakvih zasada je biološka rekutivacija devastiranih zemljišta, koja podrazumeva ponovno uspostavljanje vegetacijskoh pokrivača, biološku remedijaciju, zaštitu od erozije i uspostavljanje vetrozaštitnih pojaseva. Pored navedenih prednosti ove biljke apsorbuju toksične gasove i ugljen dioksid iz atmosfere što dovodi do uspostavljanja povoljne mikroklime i CO2 neutralne proizvodnje energije, a zahvaljujući visokom sadržaju lignoceluloznih komponenti mogu se efikasno koristiti za proizvodnju tečnih goriva - bioetanola. Ukoliko bi ove biljke bile korišćene za biološku rekultivaciju zemljišta devstiranih površina JP EPS i bile efikasno upotrebljene kao supstituent uglja u proizvodnji energije, moglo bi se zameniti do 4,6% ukupne godišnje proizvodnje uglja i uštedeti preko 1,1 milijarde samo na emisiji CO2 i dodatno ostvariti ušteda na redukciji deponovanja pepela.",
publisher = "Beograd: Savez energetičara",
journal = "Zbornik radova: 33. međunarodno savetovanje: Energetika 2017; 2017 Mar 28-31; Zlatibor, Serbia",
title = "Potencijal odabranih biljnih vrsta za formiranje energetskih zasada - koncept "biomasa kroz rekultivaciju"",
number = "3-4",
volume = "19",
pages = "41-51",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6733"
}

Tadić, V., Radonjić, V., Jakovljević, M., Marković, M., Raspor, M.,& Milošević, S.. (2017). Potencijal odabranih biljnih vrsta za formiranje energetskih zasada - koncept "biomasa kroz rekultivaciju". in Zbornik radova: 33. međunarodno savetovanje: Energetika 2017; 2017 Mar 28-31; Zlatibor, Serbia
Beograd: Savez energetičara., 19(3-4), 41-51.
https://hdl.handle.net/21.15107/rcub_ibiss_6733

Tadić V, Radonjić V, Jakovljević M, Marković M, Raspor M, Milošević S. Potencijal odabranih biljnih vrsta za formiranje energetskih zasada - koncept "biomasa kroz rekultivaciju". in Zbornik radova: 33. međunarodno savetovanje: Energetika 2017; 2017 Mar 28-31; Zlatibor, Serbia. 2017;19(3-4):41-51.
https://hdl.handle.net/21.15107/rcub_ibiss_6733 .

Tadić, Vojin, Radonjić, Vladimir, Jakovljević, Milan, Marković, Marija, Raspor, Martin, Milošević, Snežana, "Potencijal odabranih biljnih vrsta za formiranje energetskih zasada - koncept "biomasa kroz rekultivaciju"" in Zbornik radova: 33. međunarodno savetovanje: Energetika 2017; 2017 Mar 28-31; Zlatibor, Serbia, 19, no. 3-4 (2017):41-51,
https://hdl.handle.net/21.15107/rcub_ibiss_6733 .

TY  - JOUR
AU  - Jakovljevic, Milan
AU  - Lilic, Nikola
AU  - Kolonja, Bozo
AU  - Knezevic, Dinko
AU  - Petrić, Marija
AU  - Tadić, Vojin
AU  - Nedic, Mirko
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2046
AB  - The main goal of this paper is the overview of the scope and dynamics of
   biomass production as a renewable energy source for substitution of coal
   in the production of electrical energy in the Kolubara coal basin. In
   order to successfully realize this goal, it was necessary to develop a
   dynamic model of the process of coal production, overburden dumping and
   re-cultivation of dumping sites by biomass planting. The results
   obtained by simulation of the dynamic model of biomass production in
   Kolubara mine basin until year 2045 show that 6870 hectares of
   overburden waste dumps will be re-cultivated by biomass plantations.
   Biomass production modeling point out the significant benefits of
   biomass production by planting the willow Salix viminalis cultivated for
   energy purposes. Under these conditions, a 0.6\% participation of
   biomass at the end of the period of intensive coal production, year
   2037, is achieved. With the decrease of coal production to 15 million
   tons per year, this percentage steeply rises to 1.4\% in 2045. This
   amount of equivalent tons of coal from biomass can be used for coal
   substitution in the production of electrical energy.
T2  - Thermal Science
T1  - Biomass production as renewable energy resource at reclaimed Serbian lignite open-cast mines
IS  - 3
VL  - 19
DO  - 10.2298/TSCI140626014J
SP  - 823
EP  - 835
ER  - 

@article{
author = "Jakovljevic, Milan and Lilic, Nikola and Kolonja, Bozo and Knezevic, Dinko and Petrić, Marija and Tadić, Vojin and Nedic, Mirko",
year = "2015",
abstract = "The main goal of this paper is the overview of the scope and dynamics of
   biomass production as a renewable energy source for substitution of coal
   in the production of electrical energy in the Kolubara coal basin. In
   order to successfully realize this goal, it was necessary to develop a
   dynamic model of the process of coal production, overburden dumping and
   re-cultivation of dumping sites by biomass planting. The results
   obtained by simulation of the dynamic model of biomass production in
   Kolubara mine basin until year 2045 show that 6870 hectares of
   overburden waste dumps will be re-cultivated by biomass plantations.
   Biomass production modeling point out the significant benefits of
   biomass production by planting the willow Salix viminalis cultivated for
   energy purposes. Under these conditions, a 0.6\% participation of
   biomass at the end of the period of intensive coal production, year
   2037, is achieved. With the decrease of coal production to 15 million
   tons per year, this percentage steeply rises to 1.4\% in 2045. This
   amount of equivalent tons of coal from biomass can be used for coal
   substitution in the production of electrical energy.",
journal = "Thermal Science",
title = "Biomass production as renewable energy resource at reclaimed Serbian lignite open-cast mines",
number = "3",
volume = "19",
doi = "10.2298/TSCI140626014J",
pages = "823-835"
}

Jakovljevic, M., Lilic, N., Kolonja, B., Knezevic, D., Petrić, M., Tadić, V.,& Nedic, M.. (2015). Biomass production as renewable energy resource at reclaimed Serbian lignite open-cast mines. in Thermal Science, 19(3), 823-835.
https://doi.org/10.2298/TSCI140626014J

Jakovljevic M, Lilic N, Kolonja B, Knezevic D, Petrić M, Tadić V, Nedic M. Biomass production as renewable energy resource at reclaimed Serbian lignite open-cast mines. in Thermal Science. 2015;19(3):823-835.
doi:10.2298/TSCI140626014J .

Jakovljevic, Milan, Lilic, Nikola, Kolonja, Bozo, Knezevic, Dinko, Petrić, Marija, Tadić, Vojin, Nedic, Mirko, "Biomass production as renewable energy resource at reclaimed Serbian lignite open-cast mines" in Thermal Science, 19, no. 3 (2015):823-835,
https://doi.org/10.2298/TSCI140626014J . .

TY  - JOUR
AU  - Tadić, Vojin
AU  - Balaž, Ana Marija J.
AU  - Petrić, Marija
AU  - Milošević, Snežana
AU  - Zelenović, Nevena D.
AU  - Raspor, Martin
AU  - Tadić, Jovan M.
AU  - Prodanović, Radivoje M.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2339
AB  - We have cloned the gene for carbohydrate oxidase (CHO) from Lactuca
   sativa in two species of yeasts (Saccharomyces cerevisiae and Pichia
   pastoris). The synthetic gene for the carbohydrate oxidase (1821 bp)
   from L. sativa cloned into the vector pUC57 and inserted into plasmids
   pYES2 and pGAP using Escherichia coli DH5 alpha strain. The P. pastoris
   strain X-33 and the S. cerevisiae strain InvSC1 were used for
   extracellular expression of CHO. After transformation of P. pastoris
   X-33 with CHO-pGAP construct none of the colonies showed CHO activity.
   Two samples displayed a band which did not exist in the sample with the
   empty vector similar to the molecular weight of CHO. The S. cerevisiae
   strain InvSC1 has been also transformed with CHO-pYES constructs. Three
   colonies grew on the plate with cells transformed with the construct.
   One of the samples showed a band corresponding to about 110 kDa, but no
   CHO activity was recorded in this case either. Cloning of the foreign
   genes and heterologous expression in yeasts is widely used in
   biotechnology, but sometimes can be very dependent on the gene sequence
   and strain used. In order to obtain active CHO enzyme the further
   studies on purification and refolding of expressed protein are
   necessary.
T2  - Hemijska Industrija
T1  - Cloning of the gene for a carbohydrate oxidase from Lactuca sativa in
 the yeasts Saccharomyces cerevisiae and Pichia pastoris
IS  - 6
VL  - 69
DO  - 10.2298/HEMIND140823003T
SP  - 689
EP  - 701
ER  - 

@article{
author = "Tadić, Vojin and Balaž, Ana Marija J. and Petrić, Marija and Milošević, Snežana and Zelenović, Nevena D. and Raspor, Martin and Tadić, Jovan M. and Prodanović, Radivoje M.",
year = "2015",
abstract = "We have cloned the gene for carbohydrate oxidase (CHO) from Lactuca
   sativa in two species of yeasts (Saccharomyces cerevisiae and Pichia
   pastoris). The synthetic gene for the carbohydrate oxidase (1821 bp)
   from L. sativa cloned into the vector pUC57 and inserted into plasmids
   pYES2 and pGAP using Escherichia coli DH5 alpha strain. The P. pastoris
   strain X-33 and the S. cerevisiae strain InvSC1 were used for
   extracellular expression of CHO. After transformation of P. pastoris
   X-33 with CHO-pGAP construct none of the colonies showed CHO activity.
   Two samples displayed a band which did not exist in the sample with the
   empty vector similar to the molecular weight of CHO. The S. cerevisiae
   strain InvSC1 has been also transformed with CHO-pYES constructs. Three
   colonies grew on the plate with cells transformed with the construct.
   One of the samples showed a band corresponding to about 110 kDa, but no
   CHO activity was recorded in this case either. Cloning of the foreign
   genes and heterologous expression in yeasts is widely used in
   biotechnology, but sometimes can be very dependent on the gene sequence
   and strain used. In order to obtain active CHO enzyme the further
   studies on purification and refolding of expressed protein are
   necessary.",
journal = "Hemijska Industrija",
title = "Cloning of the gene for a carbohydrate oxidase from Lactuca sativa in
 the yeasts Saccharomyces cerevisiae and Pichia pastoris",
number = "6",
volume = "69",
doi = "10.2298/HEMIND140823003T",
pages = "689-701"
}

Tadić, V., Balaž, A. M. J., Petrić, M., Milošević, S., Zelenović, N. D., Raspor, M., Tadić, J. M.,& Prodanović, R. M.. (2015). Cloning of the gene for a carbohydrate oxidase from Lactuca sativa in
 the yeasts Saccharomyces cerevisiae and Pichia pastoris. in Hemijska Industrija, 69(6), 689-701.
https://doi.org/10.2298/HEMIND140823003T

Tadić V, Balaž AMJ, Petrić M, Milošević S, Zelenović ND, Raspor M, Tadić JM, Prodanović RM. Cloning of the gene for a carbohydrate oxidase from Lactuca sativa in
 the yeasts Saccharomyces cerevisiae and Pichia pastoris. in Hemijska Industrija. 2015;69(6):689-701.
doi:10.2298/HEMIND140823003T .

Tadić, Vojin, Balaž, Ana Marija J., Petrić, Marija, Milošević, Snežana, Zelenović, Nevena D., Raspor, Martin, Tadić, Jovan M., Prodanović, Radivoje M., "Cloning of the gene for a carbohydrate oxidase from Lactuca sativa in
 the yeasts Saccharomyces cerevisiae and Pichia pastoris" in Hemijska Industrija, 69, no. 6 (2015):689-701,
https://doi.org/10.2298/HEMIND140823003T . .

TY  - JOUR
AU  - Petrić, Marija
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Trifunovic-Momcilov, Milana
AU  - Tadić, Vojin
AU  - Grujic, Marica
AU  - Vujcic, Zoran
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2321
AB  - Morphogenesis in vitro is a complex and still poorly defined process. We
   investigated esterase and peroxidase isoforms detected in bulb scale,
   during Fritillaria meleagris morphogenesis. Bulbs were grown either at 4
   degrees C or on a medium with an increased concentration of sucrose
   (4.5\%) for 30 days. After these pre-treatments, the bulb scales were
   further grown on nutrient media that contained different concentrations
   of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KIN) or
   thidiazuron (TDZ). Regeneration of somatic embryos and bulblets occurred
   at the same explant. The highest numbers of somatic embryos and bulblets
   were regenerated on the medium containing 2,4-D and KIN (1 mg/L each),
   while morphogenesis was most successful at a TDZ concentration between
   0.5 and 1 mg/L. Monitoring of esterases and peroxidases was performed by
   growing bulb scales on a medium enriched with 2,4-D and KIN or TDZ (1
   mg/L), and the number and activity of isoforms were followed every 7
   days for 4 weeks. In control explants, six isoforms of esterase were
   observed. Three isoforms of peroxidase were not detected in the control
   bulb scale, which has not begun its morphogenesis process. 2015 Academie
   des sciences. (C) Published by Elsevier Masson SAS. All rights reserved.
T2  - Comptes Rendus Biologies
T1  - Esterase and peroxidase isoforms in different stages of morphogenesis in
 Fritillaria meleagris L. in bulb-scale culture
IS  - 12
VL  - 338
DO  - 10.1016/j.crvi.2015.08.002
SP  - 793
EP  - 802
ER  - 

@article{
author = "Petrić, Marija and Subotić, Angelina and Jevremović, Slađana and Trifunovic-Momcilov, Milana and Tadić, Vojin and Grujic, Marica and Vujcic, Zoran",
year = "2015",
abstract = "Morphogenesis in vitro is a complex and still poorly defined process. We
   investigated esterase and peroxidase isoforms detected in bulb scale,
   during Fritillaria meleagris morphogenesis. Bulbs were grown either at 4
   degrees C or on a medium with an increased concentration of sucrose
   (4.5\%) for 30 days. After these pre-treatments, the bulb scales were
   further grown on nutrient media that contained different concentrations
   of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KIN) or
   thidiazuron (TDZ). Regeneration of somatic embryos and bulblets occurred
   at the same explant. The highest numbers of somatic embryos and bulblets
   were regenerated on the medium containing 2,4-D and KIN (1 mg/L each),
   while morphogenesis was most successful at a TDZ concentration between
   0.5 and 1 mg/L. Monitoring of esterases and peroxidases was performed by
   growing bulb scales on a medium enriched with 2,4-D and KIN or TDZ (1
   mg/L), and the number and activity of isoforms were followed every 7
   days for 4 weeks. In control explants, six isoforms of esterase were
   observed. Three isoforms of peroxidase were not detected in the control
   bulb scale, which has not begun its morphogenesis process. 2015 Academie
   des sciences. (C) Published by Elsevier Masson SAS. All rights reserved.",
journal = "Comptes Rendus Biologies",
title = "Esterase and peroxidase isoforms in different stages of morphogenesis in
 Fritillaria meleagris L. in bulb-scale culture",
number = "12",
volume = "338",
doi = "10.1016/j.crvi.2015.08.002",
pages = "793-802"
}

Petrić, M., Subotić, A., Jevremović, S., Trifunovic-Momcilov, M., Tadić, V., Grujic, M.,& Vujcic, Z.. (2015). Esterase and peroxidase isoforms in different stages of morphogenesis in
 Fritillaria meleagris L. in bulb-scale culture. in Comptes Rendus Biologies, 338(12), 793-802.
https://doi.org/10.1016/j.crvi.2015.08.002

Petrić M, Subotić A, Jevremović S, Trifunovic-Momcilov M, Tadić V, Grujic M, Vujcic Z. Esterase and peroxidase isoforms in different stages of morphogenesis in
 Fritillaria meleagris L. in bulb-scale culture. in Comptes Rendus Biologies. 2015;338(12):793-802.
doi:10.1016/j.crvi.2015.08.002 .

Petrić, Marija, Subotić, Angelina, Jevremović, Slađana, Trifunovic-Momcilov, Milana, Tadić, Vojin, Grujic, Marica, Vujcic, Zoran, "Esterase and peroxidase isoforms in different stages of morphogenesis in
 Fritillaria meleagris L. in bulb-scale culture" in Comptes Rendus Biologies, 338, no. 12 (2015):793-802,
https://doi.org/10.1016/j.crvi.2015.08.002 . .

TY  - JOUR
AU  - Tadić, Vojin
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Petrić, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Antonić Reljin, Dragana
AU  - Jovan, Tadic
AU  - Subotić, Angelina
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2122
AB  - Hairy root cultures of lettuce (Lactuca sativa L.) were obtained by
   inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs.
   Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS.
   Approximately in 96.7\% cvs. Nansen and in 91.2\% Ljubljanska ledenka
   inoculated explants produced hairy root when they were incubated on
   Murashige and Skoog (MS) half-strength medium without plant growth
   regulators. A total of 54\% of all hairy root cultures expressed GUS
   activity. Every hairy root represented an independent transformation
   event. Line Ljubljanska ledenka 18 showed the highest biomass (5.5 times
   the biomass of control root). A PCR analysis of the genomic DNA
   confirmed the presence of marker and target genes in 15 hairy roots
   examined.
T2  - Central European Journal of Biology
T1  - Production of hairy root cultures of lettuce (Lactuca sativa L.)
IS  - 12
VL  - 9
DO  - 10.2478/s11535-014-0351-9
SP  - 1196
EP  - 1205
ER  - 

@article{
author = "Tadić, Vojin and Milošević, Snežana and Cingel, Aleksandar and Petrić, Marija and Trifunović-Momčilov, Milana and Antonić Reljin, Dragana and Jovan, Tadic and Subotić, Angelina",
year = "2014",
abstract = "Hairy root cultures of lettuce (Lactuca sativa L.) were obtained by
   inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs.
   Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS.
   Approximately in 96.7\% cvs. Nansen and in 91.2\% Ljubljanska ledenka
   inoculated explants produced hairy root when they were incubated on
   Murashige and Skoog (MS) half-strength medium without plant growth
   regulators. A total of 54\% of all hairy root cultures expressed GUS
   activity. Every hairy root represented an independent transformation
   event. Line Ljubljanska ledenka 18 showed the highest biomass (5.5 times
   the biomass of control root). A PCR analysis of the genomic DNA
   confirmed the presence of marker and target genes in 15 hairy roots
   examined.",
journal = "Central European Journal of Biology",
title = "Production of hairy root cultures of lettuce (Lactuca sativa L.)",
number = "12",
volume = "9",
doi = "10.2478/s11535-014-0351-9",
pages = "1196-1205"
}

Tadić, V., Milošević, S., Cingel, A., Petrić, M., Trifunović-Momčilov, M., Antonić Reljin, D., Jovan, T.,& Subotić, A.. (2014). Production of hairy root cultures of lettuce (Lactuca sativa L.). in Central European Journal of Biology, 9(12), 1196-1205.
https://doi.org/10.2478/s11535-014-0351-9

Tadić V, Milošević S, Cingel A, Petrić M, Trifunović-Momčilov M, Antonić Reljin D, Jovan T, Subotić A. Production of hairy root cultures of lettuce (Lactuca sativa L.). in Central European Journal of Biology. 2014;9(12):1196-1205.
doi:10.2478/s11535-014-0351-9 .

Tadić, Vojin, Milošević, Snežana, Cingel, Aleksandar, Petrić, Marija, Trifunović-Momčilov, Milana, Antonić Reljin, Dragana, Jovan, Tadic, Subotić, Angelina, "Production of hairy root cultures of lettuce (Lactuca sativa L.)" in Central European Journal of Biology, 9, no. 12 (2014):1196-1205,
https://doi.org/10.2478/s11535-014-0351-9 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Tadić, Vojin
AU  - Petrić, Marija
AU  - Paunović, Danijela
AU  - Jevremović, Slađana
AU  - Subotić, Angelina
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2222
AB  - Arabinogalactan proteins (AGPs) are a family of highly glycosylated cell
   surface proteins located at the plasma membrane and plant cell wall.
   AGPs play important roles in plant growth and development. Yariv
   phenylglycoside (beta GlcY), synthetic red-brown dye that specifically
   binds and precipitates AGPs, has been used for detection and
   quantification of AGPs in plant tissue. Graded concentrations of beta
   GlcY (0-75 mu M) were used to investigate the effect of this synthetic
   dye on induction of in vitro morphogenesis in Centaurium erythraea root
   culture on two nutrient media: A1/2MS and A1/2MS + IBA 1.0 mu M.
   Regeneration of C. erythraea shoots on root explants was stimulated on
   both media supplemented with 25 mu M beta GlcY after 8 weeks in culture.
   Quantification of AGPs in different tissues of C. erythraea was
   determinate with single radial diffusion method. This work emphasizes
   clear effect of beta GlcY on induction of morphogenesis in vitro in C.
   erythraea root culture.
T2  - Acta Physiologiae Plantarum
T1  - Quantification of arabinogalactan proteins during in vitro morphogenesis
 induced by beta-D-glucosyl Yariv reagent in Centaurium erythraea root
 culture
IS  - 5
VL  - 36
DO  - 10.1007/s11738-014-1495-y
SP  - 1187
EP  - 1195
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Tadić, Vojin and Petrić, Marija and Paunović, Danijela and Jevremović, Slađana and Subotić, Angelina",
year = "2014",
abstract = "Arabinogalactan proteins (AGPs) are a family of highly glycosylated cell
   surface proteins located at the plasma membrane and plant cell wall.
   AGPs play important roles in plant growth and development. Yariv
   phenylglycoside (beta GlcY), synthetic red-brown dye that specifically
   binds and precipitates AGPs, has been used for detection and
   quantification of AGPs in plant tissue. Graded concentrations of beta
   GlcY (0-75 mu M) were used to investigate the effect of this synthetic
   dye on induction of in vitro morphogenesis in Centaurium erythraea root
   culture on two nutrient media: A1/2MS and A1/2MS + IBA 1.0 mu M.
   Regeneration of C. erythraea shoots on root explants was stimulated on
   both media supplemented with 25 mu M beta GlcY after 8 weeks in culture.
   Quantification of AGPs in different tissues of C. erythraea was
   determinate with single radial diffusion method. This work emphasizes
   clear effect of beta GlcY on induction of morphogenesis in vitro in C.
   erythraea root culture.",
journal = "Acta Physiologiae Plantarum",
title = "Quantification of arabinogalactan proteins during in vitro morphogenesis
 induced by beta-D-glucosyl Yariv reagent in Centaurium erythraea root
 culture",
number = "5",
volume = "36",
doi = "10.1007/s11738-014-1495-y",
pages = "1187-1195"
}

Trifunović-Momčilov, M., Tadić, V., Petrić, M., Paunović, D., Jevremović, S.,& Subotić, A.. (2014). Quantification of arabinogalactan proteins during in vitro morphogenesis
 induced by beta-D-glucosyl Yariv reagent in Centaurium erythraea root
 culture. in Acta Physiologiae Plantarum, 36(5), 1187-1195.
https://doi.org/10.1007/s11738-014-1495-y

Trifunović-Momčilov M, Tadić V, Petrić M, Paunović D, Jevremović S, Subotić A. Quantification of arabinogalactan proteins during in vitro morphogenesis
 induced by beta-D-glucosyl Yariv reagent in Centaurium erythraea root
 culture. in Acta Physiologiae Plantarum. 2014;36(5):1187-1195.
doi:10.1007/s11738-014-1495-y .

Trifunović-Momčilov, Milana, Tadić, Vojin, Petrić, Marija, Paunović, Danijela, Jevremović, Slađana, Subotić, Angelina, "Quantification of arabinogalactan proteins during in vitro morphogenesis
 induced by beta-D-glucosyl Yariv reagent in Centaurium erythraea root
 culture" in Acta Physiologiae Plantarum, 36, no. 5 (2014):1187-1195,
https://doi.org/10.1007/s11738-014-1495-y . .

TY  - JOUR
AU  - Tadić, Vojin
AU  - Petrić, Marija
AU  - Milosevic, Snezana
AU  - Cingel, Aleksandar
AU  - Raspor, Martin
AU  - Spasojevic, Dragica
AU  - Tadic, Jovan
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2261
AB  - In this study we examined the activities of polyphenol oxidase (PPO) and
   antioxidant enzymes, peroxidase (POX) and catalase (CAT) during lettuce
   seed germination at different concentrations of phenol. Out of eleven
   varieties of lettuce, four were chosen according to their germination
   tolerance to phenol as follows: plants exhibiting high (Ljubljanska
   ledenka - LJL and Nansen - N) and low toleranace (Little Gem - LG and
   Majska kraljica - MK). A decrease in germination efficiency after
   exposure to LD50 of phenol was determined for these four varieties. The
   effects of phenol treatment on POX, CAT and PPO activities were
   determined after 4, 5, 6, 7 and 8 days of growth at LD50 concentrations.
   A trend of increased peroxidase activity was observed in seeds grown on
   LD50 of phenol compared to control seeds. A significant increase in CAT
   activity was observed at the beginning of treatment for MK, LG and N in
   seeds grown on phenol as well as in control seeds. A trend of increased
   PPO activity was observed in all control seeds. We also investigated the
   affinity of PPO for two different substrates that were used for the
   determination of enzyme activity. Our results show that LJL and N are
   the varieties most tolerant to growth on phenol. Here we report on the
   activities of their antioxidant enzymes and PPO during seed germination.
T2  - Archives of Biological Sciences
T1  - Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce
IS  - 4
VL  - 66
DO  - 10.2298/ABS1404503T
SP  - 1503
EP  - 1514
ER  - 

@article{
author = "Tadić, Vojin and Petrić, Marija and Milosevic, Snezana and Cingel, Aleksandar and Raspor, Martin and Spasojevic, Dragica and Tadic, Jovan",
year = "2014",
abstract = "In this study we examined the activities of polyphenol oxidase (PPO) and
   antioxidant enzymes, peroxidase (POX) and catalase (CAT) during lettuce
   seed germination at different concentrations of phenol. Out of eleven
   varieties of lettuce, four were chosen according to their germination
   tolerance to phenol as follows: plants exhibiting high (Ljubljanska
   ledenka - LJL and Nansen - N) and low toleranace (Little Gem - LG and
   Majska kraljica - MK). A decrease in germination efficiency after
   exposure to LD50 of phenol was determined for these four varieties. The
   effects of phenol treatment on POX, CAT and PPO activities were
   determined after 4, 5, 6, 7 and 8 days of growth at LD50 concentrations.
   A trend of increased peroxidase activity was observed in seeds grown on
   LD50 of phenol compared to control seeds. A significant increase in CAT
   activity was observed at the beginning of treatment for MK, LG and N in
   seeds grown on phenol as well as in control seeds. A trend of increased
   PPO activity was observed in all control seeds. We also investigated the
   affinity of PPO for two different substrates that were used for the
   determination of enzyme activity. Our results show that LJL and N are
   the varieties most tolerant to growth on phenol. Here we report on the
   activities of their antioxidant enzymes and PPO during seed germination.",
journal = "Archives of Biological Sciences",
title = "Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce",
number = "4",
volume = "66",
doi = "10.2298/ABS1404503T",
pages = "1503-1514"
}

Tadić, V., Petrić, M., Milosevic, S., Cingel, A., Raspor, M., Spasojevic, D.,& Tadic, J.. (2014). Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce. in Archives of Biological Sciences, 66(4), 1503-1514.
https://doi.org/10.2298/ABS1404503T

Tadić V, Petrić M, Milosevic S, Cingel A, Raspor M, Spasojevic D, Tadic J. Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce. in Archives of Biological Sciences. 2014;66(4):1503-1514.
doi:10.2298/ABS1404503T .

Tadić, Vojin, Petrić, Marija, Milosevic, Snezana, Cingel, Aleksandar, Raspor, Martin, Spasojevic, Dragica, Tadic, Jovan, "Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce" in Archives of Biological Sciences, 66, no. 4 (2014):1503-1514,
https://doi.org/10.2298/ABS1404503T . .

TY  - JOUR
AU  - Petrić, Marija
AU  - Jevremović, Slađana
AU  - Trifunović-Momčilov, Milana
AU  - Tadić, Vojin
AU  - Milosevic, Snezana
AU  - Subotić, Angelina
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2292
AB  - We investigated the activity of 3 antioxidant enzymes: superoxide
   dismutase, catalase, and peroxidase during morphogenesis of Fritillaria
   meleagris L. in vitro in bulb scale culture. Bulb cultures of F.
   meleagris were established on nutrition medium supplemented with 1.0 mg
   L-1 thidiazuron. Bulbs were grown at standard (24 degrees C) and low
   temperatures (4 degrees C) or at an increased sucrose concentration
   (4.5\%) during the first 6 weeks (pretreatments). All bulbs were then
   grown for 4 weeks at standard temperature on 2,4-dichlorophenoxyacetic
   acid and kinetin or thidiazuron (at concentrations of 1 mg L-1 each) in
   order to examine the influence of the above-mentioned pretreatments and
   nutrient medium composition on enzyme activity. Enzyme activity was
   measured 7, 14, 21, and 28 days after the start of morphogenesis
   induction. Superoxide dismutase and catalase showed the highest activity
   at the beginning of morphogenesis in vitro. Peroxidase activity was the
   highest in bulb segments immediately after isolation following
   pretreatments and on both nutrition media. We also determined the effect
   of pretreatment coupled with low temperature (4 degrees C) on growth and
   development of F. meleagris in vitro and chlorophyll and carotenoids
   content.
T2  - Turkish Journal of Biology
T1  - Activity of antioxidant enzymes during induction of morphogenesis of
 Fritillaria meleagris in bulb scale culture
IS  - 3
VL  - 38
DO  - 10.3906/biy-1309-45
SP  - 328
EP  - 338
ER  - 

@article{
author = "Petrić, Marija and Jevremović, Slađana and Trifunović-Momčilov, Milana and Tadić, Vojin and Milosevic, Snezana and Subotić, Angelina",
year = "2014",
abstract = "We investigated the activity of 3 antioxidant enzymes: superoxide
   dismutase, catalase, and peroxidase during morphogenesis of Fritillaria
   meleagris L. in vitro in bulb scale culture. Bulb cultures of F.
   meleagris were established on nutrition medium supplemented with 1.0 mg
   L-1 thidiazuron. Bulbs were grown at standard (24 degrees C) and low
   temperatures (4 degrees C) or at an increased sucrose concentration
   (4.5\%) during the first 6 weeks (pretreatments). All bulbs were then
   grown for 4 weeks at standard temperature on 2,4-dichlorophenoxyacetic
   acid and kinetin or thidiazuron (at concentrations of 1 mg L-1 each) in
   order to examine the influence of the above-mentioned pretreatments and
   nutrient medium composition on enzyme activity. Enzyme activity was
   measured 7, 14, 21, and 28 days after the start of morphogenesis
   induction. Superoxide dismutase and catalase showed the highest activity
   at the beginning of morphogenesis in vitro. Peroxidase activity was the
   highest in bulb segments immediately after isolation following
   pretreatments and on both nutrition media. We also determined the effect
   of pretreatment coupled with low temperature (4 degrees C) on growth and
   development of F. meleagris in vitro and chlorophyll and carotenoids
   content.",
journal = "Turkish Journal of Biology",
title = "Activity of antioxidant enzymes during induction of morphogenesis of
 Fritillaria meleagris in bulb scale culture",
number = "3",
volume = "38",
doi = "10.3906/biy-1309-45",
pages = "328-338"
}

Petrić, M., Jevremović, S., Trifunović-Momčilov, M., Tadić, V., Milosevic, S.,& Subotić, A.. (2014). Activity of antioxidant enzymes during induction of morphogenesis of
 Fritillaria meleagris in bulb scale culture. in Turkish Journal of Biology, 38(3), 328-338.
https://doi.org/10.3906/biy-1309-45

Petrić M, Jevremović S, Trifunović-Momčilov M, Tadić V, Milosevic S, Subotić A. Activity of antioxidant enzymes during induction of morphogenesis of
 Fritillaria meleagris in bulb scale culture. in Turkish Journal of Biology. 2014;38(3):328-338.
doi:10.3906/biy-1309-45 .

Petrić, Marija, Jevremović, Slađana, Trifunović-Momčilov, Milana, Tadić, Vojin, Milosevic, Snezana, Subotić, Angelina, "Activity of antioxidant enzymes during induction of morphogenesis of
 Fritillaria meleagris in bulb scale culture" in Turkish Journal of Biology, 38, no. 3 (2014):328-338,
https://doi.org/10.3906/biy-1309-45 . .