TY  - JOUR
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Oreščanin Dušić, Zorana
AU  - Milenković, Dragan
AU  - Konić-Ristić, Aleksandra
AU  - Blagojević, Duško
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka 
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S053155651630290X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2689
AB  - This study aimed to investigate effects of citrus flavanones naringenin (NAR) and hesperetin (HES) on liver antioxidant status and membrane phospholipid composition in 24-month-old rats. NAR and HES (15 mg/kg) were administrated orally to male Wistar rats, once per day, for 4 weeks. Control group received either vehicle (sunflower oil) or remained intact. The results showed decreased (p < 0.05) activity of antioxidant enzymes (AOE), specifically catalase (CAT), superoxide dismutase (SOD) 1 and glutathione reductase (GR) in the liver of intact control old-aged rats in comparison to young intact controls. Flavanone administration to old-aged males increased (p < 0.05) examined AOE activities in comparison to vehicle-administered animals. Namely, NAR was more potent in comparison to HES regarding the increase (p < 0.05) in activities of examined antioxidant enzymes (SOD 1 and 2, glutathione peroxidase-GPx and GR) and the liver glutathione (GSH), while HES elevated (p < 0.05) only activity of CAT and GR. Both flavanones significantly decreased (p < 0.05) TBARS and improved (p < 0.05) membrane phospholipid composition in favor of n-3 PUFA and n-6/n-3 PUFA ratio. Both flavanones did not affect liver histology and reduced (p < 0.05) alanine aminotransferase and aspartate aminotransferase levels in serum. The results of this study indicate beneficial potential of citrus flavanones in the old-aged rat liver.
T2  - Experimental Gerontology
T1  - Citrus flavanones naringenin and hesperetin improve antioxidant status and membrane lipid compositions in the liver of old-aged Wistar rats
VL  - 84
DO  - 10.1016/j.exger.2016.08.014
SP  - 49
EP  - 60
ER  - 

@article{
author = "Miler, Marko and Živanović, Jasmina and Ajdžanović, Vladimir and Oreščanin Dušić, Zorana and Milenković, Dragan and Konić-Ristić, Aleksandra and Blagojević, Duško and Milošević, Verica and Šošić-Jurjević, Branka ",
year = "2016",
abstract = "This study aimed to investigate effects of citrus flavanones naringenin (NAR) and hesperetin (HES) on liver antioxidant status and membrane phospholipid composition in 24-month-old rats. NAR and HES (15 mg/kg) were administrated orally to male Wistar rats, once per day, for 4 weeks. Control group received either vehicle (sunflower oil) or remained intact. The results showed decreased (p < 0.05) activity of antioxidant enzymes (AOE), specifically catalase (CAT), superoxide dismutase (SOD) 1 and glutathione reductase (GR) in the liver of intact control old-aged rats in comparison to young intact controls. Flavanone administration to old-aged males increased (p < 0.05) examined AOE activities in comparison to vehicle-administered animals. Namely, NAR was more potent in comparison to HES regarding the increase (p < 0.05) in activities of examined antioxidant enzymes (SOD 1 and 2, glutathione peroxidase-GPx and GR) and the liver glutathione (GSH), while HES elevated (p < 0.05) only activity of CAT and GR. Both flavanones significantly decreased (p < 0.05) TBARS and improved (p < 0.05) membrane phospholipid composition in favor of n-3 PUFA and n-6/n-3 PUFA ratio. Both flavanones did not affect liver histology and reduced (p < 0.05) alanine aminotransferase and aspartate aminotransferase levels in serum. The results of this study indicate beneficial potential of citrus flavanones in the old-aged rat liver.",
journal = "Experimental Gerontology",
title = "Citrus flavanones naringenin and hesperetin improve antioxidant status and membrane lipid compositions in the liver of old-aged Wistar rats",
volume = "84",
doi = "10.1016/j.exger.2016.08.014",
pages = "49-60"
}

Miler, M., Živanović, J., Ajdžanović, V., Oreščanin Dušić, Z., Milenković, D., Konić-Ristić, A., Blagojević, D., Milošević, V.,& Šošić-Jurjević, B.. (2016). Citrus flavanones naringenin and hesperetin improve antioxidant status and membrane lipid compositions in the liver of old-aged Wistar rats. in Experimental Gerontology, 84, 49-60.
https://doi.org/10.1016/j.exger.2016.08.014

Miler M, Živanović J, Ajdžanović V, Oreščanin Dušić Z, Milenković D, Konić-Ristić A, Blagojević D, Milošević V, Šošić-Jurjević B. Citrus flavanones naringenin and hesperetin improve antioxidant status and membrane lipid compositions in the liver of old-aged Wistar rats. in Experimental Gerontology. 2016;84:49-60.
doi:10.1016/j.exger.2016.08.014 .

Miler, Marko, Živanović, Jasmina, Ajdžanović, Vladimir, Oreščanin Dušić, Zorana, Milenković, Dragan, Konić-Ristić, Aleksandra, Blagojević, Duško, Milošević, Verica, Šošić-Jurjević, Branka , "Citrus flavanones naringenin and hesperetin improve antioxidant status and membrane lipid compositions in the liver of old-aged Wistar rats" in Experimental Gerontology, 84 (2016):49-60,
https://doi.org/10.1016/j.exger.2016.08.014 . .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Spasojević, Ivan
AU  - Konić-Ristić, Aleksandra
AU  - Milenković, Dragan
AU  - Živanović, Jasmina
AU  - Jarić, Ivana
AU  - Milošević, Verica
PY  - 2014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6241
AB  - Superficial membrane fluidity is related to erythrocyte deformation behavior and affects the hemodynamics, especially in hypertension and atherosclerosis. Grapefruit flavanone naringenin, which is used as alternative therapeutic of cardiovascular conditions, can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of two nutritional doses of naringenin (lower - 0.1 µg/ml and higher - 1 µg/ml) on superficial membrane fluidity in human erythrocytes, which reflects their deformability. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 7-DS), the spectra of which are dependent on membrane fluidity. The results showed a significant (p=0.029) increase of membrane fluidity near the hydrophilic surface in erythrocytes treated with higher nutritional dose of naringenin, while the lower nutritional dose of naringenin was ineffective (p>0.05) in that membrane region. In the hydrophobic layer just below the erythrocyte membrane phospholipid heads both lower and higher dose of naringenin significantly (p=0.036 and p=0.028, respectively) increased membrane fluidity. These data document the positive and dose dependent effect of naringenin on superficial membrane fluidity in human erythrocytes, recommending its use in the cardiovascular conditions characterized by disturbed hemodynamics.
PB  - Belgrade: Serbian Physiological Society
C3  - Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia
T1  - The positive effect of naringenin on superficial membrane fluidity in human erythrocytes
SP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6241
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Spasojević, Ivan and Konić-Ristić, Aleksandra and Milenković, Dragan and Živanović, Jasmina and Jarić, Ivana and Milošević, Verica",
year = "2014",
abstract = "Superficial membrane fluidity is related to erythrocyte deformation behavior and affects the hemodynamics, especially in hypertension and atherosclerosis. Grapefruit flavanone naringenin, which is used as alternative therapeutic of cardiovascular conditions, can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of two nutritional doses of naringenin (lower - 0.1 µg/ml and higher - 1 µg/ml) on superficial membrane fluidity in human erythrocytes, which reflects their deformability. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 7-DS), the spectra of which are dependent on membrane fluidity. The results showed a significant (p=0.029) increase of membrane fluidity near the hydrophilic surface in erythrocytes treated with higher nutritional dose of naringenin, while the lower nutritional dose of naringenin was ineffective (p>0.05) in that membrane region. In the hydrophobic layer just below the erythrocyte membrane phospholipid heads both lower and higher dose of naringenin significantly (p=0.036 and p=0.028, respectively) increased membrane fluidity. These data document the positive and dose dependent effect of naringenin on superficial membrane fluidity in human erythrocytes, recommending its use in the cardiovascular conditions characterized by disturbed hemodynamics.",
publisher = "Belgrade: Serbian Physiological Society",
journal = "Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia",
title = "The positive effect of naringenin on superficial membrane fluidity in human erythrocytes",
pages = "63",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6241"
}

Ajdžanović, V., Spasojević, I., Konić-Ristić, A., Milenković, D., Živanović, J., Jarić, I.,& Milošević, V.. (2014). The positive effect of naringenin on superficial membrane fluidity in human erythrocytes. in Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia
Belgrade: Serbian Physiological Society., 63.
https://hdl.handle.net/21.15107/rcub_ibiss_6241

Ajdžanović V, Spasojević I, Konić-Ristić A, Milenković D, Živanović J, Jarić I, Milošević V. The positive effect of naringenin on superficial membrane fluidity in human erythrocytes. in Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia. 2014;:63.
https://hdl.handle.net/21.15107/rcub_ibiss_6241 .

Ajdžanović, Vladimir, Spasojević, Ivan, Konić-Ristić, Aleksandra, Milenković, Dragan, Živanović, Jasmina, Jarić, Ivana, Milošević, Verica, "The positive effect of naringenin on superficial membrane fluidity in human erythrocytes" in Abstract book: Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia (2014):63,
https://hdl.handle.net/21.15107/rcub_ibiss_6241 .