Sekulić, Milka

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The effects of sex steroids on thyroid C cells and trabecular bone structure in the rat model of male osteoporosis

Filipović, Branko; Šošić-Jurjević, Branka; Ajdžanović, Vladimir; Živanović, Jasmina; Nestorović, Nataša; Milošević, Verica; Sekulić, Milka

(John Wiley and Sons, 2013)

TY  - JOUR
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Nestorović, Nataša
AU  - Milošević, Verica
AU  - Sekulić, Milka
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6223
AB  - Androgen deficiency is one of the major factors leading to the development of osteoporosis in men. Since calcitonin (CT) is a potent antiresorptive agent, in the present study we investigated the effects of androgen deficiency and subsequent testosterone and estradiol treatment on CT-producing thyroid C cells, skeletal and hormonal changes in middle-aged orchidectomized (Orx) rats. Fifteen-month-old male Wistar rats were either Orx or sham-operated (SO). One group of Orx rats received 5 mg kg-1 b.w. testosterone propionate (TP) subcutaneously, while another group was injected with 0.06 mg kg-1 b.w. estradiol dipropionate (EDP) once a day for 3 weeks. A peroxidase–antiperoxidase method was applied for localization of CT in the C cells. The studies included ultrastructural microscopic observation of these cells. The metaphyseal region of the proximal tibia was measured histomorphometrically using an IMAGEJ public domain image processing program. TP or EDP treatment significantly increased C cell volume (Vc), volume densities (Vv) and serum CT concentration compared with the Orx animals. Administration of both TP and EDP significantly enhanced cancellous bone area (B.Ar), trabecular thickness (Tb.Th) and trabecular number (Tb.N) and reduced trabecular separation (Tb.Sp). Serum osteocalcin (OC) and urinary Ca concentrations were significantly lower after these treatments in comparison with Orx rats. These data suggest that testosterone and estradiol treatment in Orx middle-aged rats affect calcitonin-producing thyroid C cells, which may contribute to the bone protective effects of sex hormones in the rat model of male osteoporosis.
PB  - John Wiley and Sons
T2  - Journal of Anatomy
T1  - The effects of sex steroids on thyroid C cells and trabecular bone structure in the rat model of male osteoporosis
IS  - 3
VL  - 222
DO  - 10.1111/joa.12013
SP  - 313
EP  - 320
ER  - 
@article{
author = "Filipović, Branko and Šošić-Jurjević, Branka and Ajdžanović, Vladimir and Živanović, Jasmina and Nestorović, Nataša and Milošević, Verica and Sekulić, Milka",
year = "2013",
abstract = "Androgen deficiency is one of the major factors leading to the development of osteoporosis in men. Since calcitonin (CT) is a potent antiresorptive agent, in the present study we investigated the effects of androgen deficiency and subsequent testosterone and estradiol treatment on CT-producing thyroid C cells, skeletal and hormonal changes in middle-aged orchidectomized (Orx) rats. Fifteen-month-old male Wistar rats were either Orx or sham-operated (SO). One group of Orx rats received 5 mg kg-1 b.w. testosterone propionate (TP) subcutaneously, while another group was injected with 0.06 mg kg-1 b.w. estradiol dipropionate (EDP) once a day for 3 weeks. A peroxidase–antiperoxidase method was applied for localization of CT in the C cells. The studies included ultrastructural microscopic observation of these cells. The metaphyseal region of the proximal tibia was measured histomorphometrically using an IMAGEJ public domain image processing program. TP or EDP treatment significantly increased C cell volume (Vc), volume densities (Vv) and serum CT concentration compared with the Orx animals. Administration of both TP and EDP significantly enhanced cancellous bone area (B.Ar), trabecular thickness (Tb.Th) and trabecular number (Tb.N) and reduced trabecular separation (Tb.Sp). Serum osteocalcin (OC) and urinary Ca concentrations were significantly lower after these treatments in comparison with Orx rats. These data suggest that testosterone and estradiol treatment in Orx middle-aged rats affect calcitonin-producing thyroid C cells, which may contribute to the bone protective effects of sex hormones in the rat model of male osteoporosis.",
publisher = "John Wiley and Sons",
journal = "Journal of Anatomy",
title = "The effects of sex steroids on thyroid C cells and trabecular bone structure in the rat model of male osteoporosis",
number = "3",
volume = "222",
doi = "10.1111/joa.12013",
pages = "313-320"
}
Filipović, B., Šošić-Jurjević, B., Ajdžanović, V., Živanović, J., Nestorović, N., Milošević, V.,& Sekulić, M.. (2013). The effects of sex steroids on thyroid C cells and trabecular bone structure in the rat model of male osteoporosis. in Journal of Anatomy
John Wiley and Sons., 222(3), 313-320.
https://doi.org/10.1111/joa.12013
Filipović B, Šošić-Jurjević B, Ajdžanović V, Živanović J, Nestorović N, Milošević V, Sekulić M. The effects of sex steroids on thyroid C cells and trabecular bone structure in the rat model of male osteoporosis. in Journal of Anatomy. 2013;222(3):313-320.
doi:10.1111/joa.12013 .
Filipović, Branko, Šošić-Jurjević, Branka, Ajdžanović, Vladimir, Živanović, Jasmina, Nestorović, Nataša, Milošević, Verica, Sekulić, Milka, "The effects of sex steroids on thyroid C cells and trabecular bone structure in the rat model of male osteoporosis" in Journal of Anatomy, 222, no. 3 (2013):313-320,
https://doi.org/10.1111/joa.12013 . .
1
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18

Soybean Phytoestrogens – Friends or Foes?

Šošić-Jurjević, Branka; Filipović, Branko; Sekulić, Milka

(Rijeka: In Tech, 2011)

TY  - CHAP
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
AU  - Sekulić, Milka
PY  - 2011
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6008
AB  - Proper and balanced nutrition is very important in prevention and treatment of chronic diseases. Many individuals modify their diet and/or take different nutraceuticals expecting to attain optimum health, extend their lifespan and prevent diseases such as cardiovascular, cancer, osteoporosis, obesity, or diabetes type II. Based on „Japanese phenomenon“ (Adlercreutz, 1998), numerous advertisements suggest that soy-based diet, and its phytoestrogens (PE) in particular, provide protection against many chronic diseases and contribute to the long lifespan often observed in Asia. That is why soy and other phytoestrogen rich plants became increasingly popular in the U.S. and western countries in the past 30 years. Furthermore, in these countries, PEs are often consumed in its purified form, as nutritional supplements, “designed” for special medical purposes. These supplements are freely available in pharmacies, health food shops, grocery shops and are usually consumed without medical control. There is a lack of awareness that uncontrolled consumption of natural PEs may be potentially harmful to human health. Even more concerning is that some people consume supplements in excess of suggested daily dosage (Wuttke et al., 2007). The soybean (Glycine max), compared to other legumes, is richer in protein levels and quality, based on its digestibility and concentration of essential amino acids (Rand et al., 2003). It is also good source of fiber, certain vitamins and minerals, such as folate and potassium (Rochfort and Panozzo, 2007). It has very high antioxidant content, similar to fruits famous for their antioxidant activity (Galleano et al., 2010). Also, despite their high carbohydrate content, the glycemic load of soybeans is relatively low due to their low glycemic index. In addition, soy-food has high levels of iron in the form of ferritin (Lonnerdal et al., 2006). The concentration of calcium in soymilk is much lower than in cow milk, however, its absorption from soy milk is similar to that from cow milk (Reinwald and Weaver, 2010). Besides the favorable nutritional attributes, soybean contains a number of biologically active components (saponins and lunasin, phytic acids, phytosterols, trypsin inhibitors, and peptides) including isoflavones genistein (G), daidzein (D) and glycitein (Gy). As soybean phytoestrogens, isoflavones are considered the most important in prevention and treatment of hormone-dependent cancers, cardiovascular diseases, osteoporosis, menopausal symptoms and other age-related diseases. In addition, some studies suggest that soy and its isoflavones affect body weight homeostasis. Modern world is a controversy with ever-increasing obesity on one side, and a high percent of starving people around the globe, on the other side. Having that in mind, combined with...
PB  - Rijeka: In Tech
T2  - Recent Trends for Enhancing the Diversity and Quality of Soybean Products
T1  - Soybean Phytoestrogens – Friends or Foes?
DO  - 10.5772/19078
SP  - 19078
SP  - 131
EP  - 174
ER  - 
@inbook{
author = "Šošić-Jurjević, Branka and Filipović, Branko and Sekulić, Milka",
year = "2011",
abstract = "Proper and balanced nutrition is very important in prevention and treatment of chronic diseases. Many individuals modify their diet and/or take different nutraceuticals expecting to attain optimum health, extend their lifespan and prevent diseases such as cardiovascular, cancer, osteoporosis, obesity, or diabetes type II. Based on „Japanese phenomenon“ (Adlercreutz, 1998), numerous advertisements suggest that soy-based diet, and its phytoestrogens (PE) in particular, provide protection against many chronic diseases and contribute to the long lifespan often observed in Asia. That is why soy and other phytoestrogen rich plants became increasingly popular in the U.S. and western countries in the past 30 years. Furthermore, in these countries, PEs are often consumed in its purified form, as nutritional supplements, “designed” for special medical purposes. These supplements are freely available in pharmacies, health food shops, grocery shops and are usually consumed without medical control. There is a lack of awareness that uncontrolled consumption of natural PEs may be potentially harmful to human health. Even more concerning is that some people consume supplements in excess of suggested daily dosage (Wuttke et al., 2007). The soybean (Glycine max), compared to other legumes, is richer in protein levels and quality, based on its digestibility and concentration of essential amino acids (Rand et al., 2003). It is also good source of fiber, certain vitamins and minerals, such as folate and potassium (Rochfort and Panozzo, 2007). It has very high antioxidant content, similar to fruits famous for their antioxidant activity (Galleano et al., 2010). Also, despite their high carbohydrate content, the glycemic load of soybeans is relatively low due to their low glycemic index. In addition, soy-food has high levels of iron in the form of ferritin (Lonnerdal et al., 2006). The concentration of calcium in soymilk is much lower than in cow milk, however, its absorption from soy milk is similar to that from cow milk (Reinwald and Weaver, 2010). Besides the favorable nutritional attributes, soybean contains a number of biologically active components (saponins and lunasin, phytic acids, phytosterols, trypsin inhibitors, and peptides) including isoflavones genistein (G), daidzein (D) and glycitein (Gy). As soybean phytoestrogens, isoflavones are considered the most important in prevention and treatment of hormone-dependent cancers, cardiovascular diseases, osteoporosis, menopausal symptoms and other age-related diseases. In addition, some studies suggest that soy and its isoflavones affect body weight homeostasis. Modern world is a controversy with ever-increasing obesity on one side, and a high percent of starving people around the globe, on the other side. Having that in mind, combined with...",
publisher = "Rijeka: In Tech",
journal = "Recent Trends for Enhancing the Diversity and Quality of Soybean Products",
booktitle = "Soybean Phytoestrogens – Friends or Foes?",
doi = "10.5772/19078",
pages = "19078-131-174"
}
Šošić-Jurjević, B., Filipović, B.,& Sekulić, M.. (2011). Soybean Phytoestrogens – Friends or Foes?. in Recent Trends for Enhancing the Diversity and Quality of Soybean Products
Rijeka: In Tech., 19078-174.
https://doi.org/10.5772/19078
Šošić-Jurjević B, Filipović B, Sekulić M. Soybean Phytoestrogens – Friends or Foes?. in Recent Trends for Enhancing the Diversity and Quality of Soybean Products. 2011;:19078-174.
doi:10.5772/19078 .
Šošić-Jurjević, Branka, Filipović, Branko, Sekulić, Milka, "Soybean Phytoestrogens – Friends or Foes?" in Recent Trends for Enhancing the Diversity and Quality of Soybean Products (2011):19078-174,
https://doi.org/10.5772/19078 . .

Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats

Filipović, Branko; Šošić-Jurjević, Branka; Ajdžanović, Vladimir; Brkić, Dejan; Manojlović Stojanoski, Milica; Milošević, Verica; Sekulić, Milka

(Springer Nature, 2010)

TY  - JOUR
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka
AU  - Ajdžanović, Vladimir
AU  - Brkić, Dejan
AU  - Manojlović Stojanoski, Milica
AU  - Milošević, Verica
AU  - Sekulić, Milka
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6131
AB  - Thyroid C cells hormone, calcitonine, inhibits bone resorption. We have demonstrated that daidzein treatment of orchidectomized rats (model for osteoporosis) stimulated C cells and increased trabecular bone mass. These results suggest that, besides direct action, daidzein may also affect bone structure indirectly through enhancement of thyroid C cell activity. Thyroid C cells produce calcitonin (CT) which acts as an inhibitor of bone resorption. In this study, the influence of daidzein treatment on thyroid C cells, bone structure, and bone function in orchidectomized (Orx) middle-aged rats was investigated. Sixteen-month-old Wistar rats were divided into Orx and sham-operated (SO) groups. Half the Orx rats were given subcutaneous injections of daidzein (30 mg/kg b.w./day) for 3 weeks. CT-immunopositive thyroid C cells were morphometrically analyzed. The metaphyseal region of the proximal tibia was measured histomorphometrically, and cancellous bone area (B.Ar), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) were calculated. Serum samples were analyzed for CT and osteocalcin (OC), calcium (Ca) and phosphorus concentrations, and urine samples for Ca levels. Treatment of Orx animals with daidzein significantly increased volume of C cells compared to the Orx rats. Daidzein also enhanced B.Ar, Tb.Th, and Tb.N and reduced Tb.Sp. The serum OC and urinary Ca concentrations decreased significantly in comparison with the Orx group. These findings indicate that daidzein treatment stimulates thyroid C cells, increase trabecular bone mass, and decrease bone turnover in Orx middle-aged rats, which is the model of male osteoporosis.
PB  - Springer Nature
T2  - Osteoporosis International
T1  - Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats
IS  - 9
VL  - 21
DO  - 10.1007/s00198-009-1092-x
SP  - 1609
EP  - 1616
ER  - 
@article{
author = "Filipović, Branko and Šošić-Jurjević, Branka and Ajdžanović, Vladimir and Brkić, Dejan and Manojlović Stojanoski, Milica and Milošević, Verica and Sekulić, Milka",
year = "2010",
abstract = "Thyroid C cells hormone, calcitonine, inhibits bone resorption. We have demonstrated that daidzein treatment of orchidectomized rats (model for osteoporosis) stimulated C cells and increased trabecular bone mass. These results suggest that, besides direct action, daidzein may also affect bone structure indirectly through enhancement of thyroid C cell activity. Thyroid C cells produce calcitonin (CT) which acts as an inhibitor of bone resorption. In this study, the influence of daidzein treatment on thyroid C cells, bone structure, and bone function in orchidectomized (Orx) middle-aged rats was investigated. Sixteen-month-old Wistar rats were divided into Orx and sham-operated (SO) groups. Half the Orx rats were given subcutaneous injections of daidzein (30 mg/kg b.w./day) for 3 weeks. CT-immunopositive thyroid C cells were morphometrically analyzed. The metaphyseal region of the proximal tibia was measured histomorphometrically, and cancellous bone area (B.Ar), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) were calculated. Serum samples were analyzed for CT and osteocalcin (OC), calcium (Ca) and phosphorus concentrations, and urine samples for Ca levels. Treatment of Orx animals with daidzein significantly increased volume of C cells compared to the Orx rats. Daidzein also enhanced B.Ar, Tb.Th, and Tb.N and reduced Tb.Sp. The serum OC and urinary Ca concentrations decreased significantly in comparison with the Orx group. These findings indicate that daidzein treatment stimulates thyroid C cells, increase trabecular bone mass, and decrease bone turnover in Orx middle-aged rats, which is the model of male osteoporosis.",
publisher = "Springer Nature",
journal = "Osteoporosis International",
title = "Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats",
number = "9",
volume = "21",
doi = "10.1007/s00198-009-1092-x",
pages = "1609-1616"
}
Filipović, B., Šošić-Jurjević, B., Ajdžanović, V., Brkić, D., Manojlović Stojanoski, M., Milošević, V.,& Sekulić, M.. (2010). Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats. in Osteoporosis International
Springer Nature., 21(9), 1609-1616.
https://doi.org/10.1007/s00198-009-1092-x
Filipović B, Šošić-Jurjević B, Ajdžanović V, Brkić D, Manojlović Stojanoski M, Milošević V, Sekulić M. Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats. in Osteoporosis International. 2010;21(9):1609-1616.
doi:10.1007/s00198-009-1092-x .
Filipović, Branko, Šošić-Jurjević, Branka, Ajdžanović, Vladimir, Brkić, Dejan, Manojlović Stojanoski, Milica, Milošević, Verica, Sekulić, Milka, "Daidzein administration positively affects thyroid C cells and bone structure in orchidectomized middle-aged rats" in Osteoporosis International, 21, no. 9 (2010):1609-1616,
https://doi.org/10.1007/s00198-009-1092-x . .
28
19
33

Pituitary ACTH cells in female rats after neonatal treatment with SRIH-14

Milošević, Verica; Nestorović, Nataša; Terzić, Milica; Ristić, Nataša; Ajdžanović, Vladimir; Trifunović, Svetlana; Sekulić, Milka

(VIA MEDICA, 2009)

TY  - JOUR
AU  - Milošević, Verica
AU  - Nestorović, Nataša
AU  - Terzić, Milica
AU  - Ristić, Nataša
AU  - Ajdžanović, Vladimir
AU  - Trifunović, Svetlana
AU  - Sekulić, Milka
PY  - 2009
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6053
AB  - The prolonged effects of neonatal SRIH-14 treatment on pituitary ACTH cells were investigated. Neonatal female rats were injected subcutaneously with SRIH (20 μg/100g b.w.) every 12 hours for five consecutive days (3rd-7th day of life). Groups of rats were then killed at the juvenile (16th day), peripubertal (38th day) or adult (80th day) stage. ACTH cells were visualized using the peroxidase-antiperoxidase immunocytochemical procedure. Morphometry and stereology were used to evaluate the ACTH-immunoreactive cell volume and volume density. The histological and immunocytochemical characteristics of ACTH cells in neonatally treated females were changed in all examined periods. Thus, SRIH-14 induced significant (p<0.05) decreases of ACTH cell volume in juvenile, peripubertal and adult rats by 26%, 39% and 14%, respectively, in comparison to the corresponding controls. The volume density of ACTH cells was also diminished (by 31%; p<0.05) at the juvenile stage in comparison with the corresponding controls. In peripubertal and adult rats, the volume densities of ACTH cells were somewhat lower (17% and 14%, respectively), but the decreases did not reach statistical significance. These findings suggest that neonatal treatment with SRIH-14 exerts a marked prolonged inhibitory effect on ACTH cell
morphology extending to the adult period of life.
PB  - VIA MEDICA
T2  - Folia Histochemica et Cytobiologica
T1  - Pituitary ACTH cells in female rats after neonatal treatment with SRIH-14
IS  - 3
VL  - 47
DO  - 10.2478/v10042-009-0104-1
SP  - 479
EP  - 484
ER  - 
@article{
author = "Milošević, Verica and Nestorović, Nataša and Terzić, Milica and Ristić, Nataša and Ajdžanović, Vladimir and Trifunović, Svetlana and Sekulić, Milka",
year = "2009",
abstract = "The prolonged effects of neonatal SRIH-14 treatment on pituitary ACTH cells were investigated. Neonatal female rats were injected subcutaneously with SRIH (20 μg/100g b.w.) every 12 hours for five consecutive days (3rd-7th day of life). Groups of rats were then killed at the juvenile (16th day), peripubertal (38th day) or adult (80th day) stage. ACTH cells were visualized using the peroxidase-antiperoxidase immunocytochemical procedure. Morphometry and stereology were used to evaluate the ACTH-immunoreactive cell volume and volume density. The histological and immunocytochemical characteristics of ACTH cells in neonatally treated females were changed in all examined periods. Thus, SRIH-14 induced significant (p<0.05) decreases of ACTH cell volume in juvenile, peripubertal and adult rats by 26%, 39% and 14%, respectively, in comparison to the corresponding controls. The volume density of ACTH cells was also diminished (by 31%; p<0.05) at the juvenile stage in comparison with the corresponding controls. In peripubertal and adult rats, the volume densities of ACTH cells were somewhat lower (17% and 14%, respectively), but the decreases did not reach statistical significance. These findings suggest that neonatal treatment with SRIH-14 exerts a marked prolonged inhibitory effect on ACTH cell
morphology extending to the adult period of life.",
publisher = "VIA MEDICA",
journal = "Folia Histochemica et Cytobiologica",
title = "Pituitary ACTH cells in female rats after neonatal treatment with SRIH-14",
number = "3",
volume = "47",
doi = "10.2478/v10042-009-0104-1",
pages = "479-484"
}
Milošević, V., Nestorović, N., Terzić, M., Ristić, N., Ajdžanović, V., Trifunović, S.,& Sekulić, M.. (2009). Pituitary ACTH cells in female rats after neonatal treatment with SRIH-14. in Folia Histochemica et Cytobiologica
VIA MEDICA., 47(3), 479-484.
https://doi.org/10.2478/v10042-009-0104-1
Milošević V, Nestorović N, Terzić M, Ristić N, Ajdžanović V, Trifunović S, Sekulić M. Pituitary ACTH cells in female rats after neonatal treatment with SRIH-14. in Folia Histochemica et Cytobiologica. 2009;47(3):479-484.
doi:10.2478/v10042-009-0104-1 .
Milošević, Verica, Nestorović, Nataša, Terzić, Milica, Ristić, Nataša, Ajdžanović, Vladimir, Trifunović, Svetlana, Sekulić, Milka, "Pituitary ACTH cells in female rats after neonatal treatment with SRIH-14" in Folia Histochemica et Cytobiologica, 47, no. 3 (2009):479-484,
https://doi.org/10.2478/v10042-009-0104-1 . .
1
1

Effect of Estradiol and Progesterone on Thyroid Gland in Pigs: A Histochemical, Stereological, and Ultrastructural Study

Sekulić, Milka; Šošić-Jurjević, Branka; Filipović, Branko; Nestorović, Nataša; Ristić, Nataša; Manojlović-Stojanoski, Milica; Milošević, Verica

(Hoboken: Wiley, 2007)

TY  - JOUR
AU  - Sekulić, Milka
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Milošević, Verica
PY  - 2007
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6098
AB  - The cellular and subcellular organization of thyroid follicular cells in peripubertal (6-month-old) male pigs treated with estradiol-dipropionate (Oe) plus progesterone (Pr) in combination on the first postnatal day was studied. A stereological method was used for morphometric determinations of the relative volume densities of the follicular epithelium, colloid and interstitium, and for establishing the epithelial height and index of activation rate. Statistically significant differences of the examined parameters between the control and Oe+Pr -treated groups were determined by Student's t-test. The subcellular organization of thyroid follicular cells was studied using transmission electron microscopy (TEM). When compared with the control group, in the Oe+Pr -treated pigs, thyroid follicles possessed a noticeably higher follicular epithelium when compared with the control animals. The observed changes were quantified and comparison between the experimental groups showed that the height, volume density of follicular epithelium, and index of activation rate were significantly (P < 0.05) increased, whereas the relative volume density of the colloid was significantly (P < 0.05) decreased. At the subcellular level the thyroid follicular cells of Oe+Pr -treated pigs were characterized by increased number of polysomes and dense bodies and extensive endoplasmatic reticulum. It was concluded that a single neonatal treatment with female gonadal steroids exerted a prolonged effect on the pig's thyroid, characterized by increased biosynthesis and reabsorption of the colloid by the follicular cells.
PB  - Hoboken: Wiley
T2  - Microscopy Research and Technique
T1  - Effect of Estradiol and Progesterone on Thyroid Gland in Pigs: A Histochemical, Stereological, and Ultrastructural Study
VL  - 70
DO  - 10.1002/jemt.20384
SP  - 44
EP  - 49
ER  - 
@article{
author = "Sekulić, Milka and Šošić-Jurjević, Branka and Filipović, Branko and Nestorović, Nataša and Ristić, Nataša and Manojlović-Stojanoski, Milica and Milošević, Verica",
year = "2007",
abstract = "The cellular and subcellular organization of thyroid follicular cells in peripubertal (6-month-old) male pigs treated with estradiol-dipropionate (Oe) plus progesterone (Pr) in combination on the first postnatal day was studied. A stereological method was used for morphometric determinations of the relative volume densities of the follicular epithelium, colloid and interstitium, and for establishing the epithelial height and index of activation rate. Statistically significant differences of the examined parameters between the control and Oe+Pr -treated groups were determined by Student's t-test. The subcellular organization of thyroid follicular cells was studied using transmission electron microscopy (TEM). When compared with the control group, in the Oe+Pr -treated pigs, thyroid follicles possessed a noticeably higher follicular epithelium when compared with the control animals. The observed changes were quantified and comparison between the experimental groups showed that the height, volume density of follicular epithelium, and index of activation rate were significantly (P < 0.05) increased, whereas the relative volume density of the colloid was significantly (P < 0.05) decreased. At the subcellular level the thyroid follicular cells of Oe+Pr -treated pigs were characterized by increased number of polysomes and dense bodies and extensive endoplasmatic reticulum. It was concluded that a single neonatal treatment with female gonadal steroids exerted a prolonged effect on the pig's thyroid, characterized by increased biosynthesis and reabsorption of the colloid by the follicular cells.",
publisher = "Hoboken: Wiley",
journal = "Microscopy Research and Technique",
title = "Effect of Estradiol and Progesterone on Thyroid Gland in Pigs: A Histochemical, Stereological, and Ultrastructural Study",
volume = "70",
doi = "10.1002/jemt.20384",
pages = "44-49"
}
Sekulić, M., Šošić-Jurjević, B., Filipović, B., Nestorović, N., Ristić, N., Manojlović-Stojanoski, M.,& Milošević, V.. (2007). Effect of Estradiol and Progesterone on Thyroid Gland in Pigs: A Histochemical, Stereological, and Ultrastructural Study. in Microscopy Research and Technique
Hoboken: Wiley., 70, 44-49.
https://doi.org/10.1002/jemt.20384
Sekulić M, Šošić-Jurjević B, Filipović B, Nestorović N, Ristić N, Manojlović-Stojanoski M, Milošević V. Effect of Estradiol and Progesterone on Thyroid Gland in Pigs: A Histochemical, Stereological, and Ultrastructural Study. in Microscopy Research and Technique. 2007;70:44-49.
doi:10.1002/jemt.20384 .
Sekulić, Milka, Šošić-Jurjević, Branka, Filipović, Branko, Nestorović, Nataša, Ristić, Nataša, Manojlović-Stojanoski, Milica, Milošević, Verica, "Effect of Estradiol and Progesterone on Thyroid Gland in Pigs: A Histochemical, Stereological, and Ultrastructural Study" in Microscopy Research and Technique, 70 (2007):44-49,
https://doi.org/10.1002/jemt.20384 . .
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Genistein i Daidzein snižavaju nivo holesterola u serumu starih orhidektomisanih pacova

Brkić, Dejan; Šošić-Jurjević, Branka ; Filipović, Branko; Ajdžanović, Vladimir; Sekulić, Milka

(Beograd: Udruženje farmaceuta NR Srbije, 2006)

TY  - CONF
AU  - Brkić, Dejan
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Sekulić, Milka
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/233
PB  - Beograd: Udruženje farmaceuta NR Srbije
C3  - 4. kongres farmaceuta Srbije sa međunarodnim učešćem: Savremena farmacija: unapređenje zdravlja i kvaliteta života; 2006 Nov 28 - Dec 2; Belgrade, Serbia
T1  - Genistein i Daidzein snižavaju nivo holesterola u serumu starih orhidektomisanih pacova
T1  - Genistein and Daidzein reduces level of total serum cholesterol in orchidectomized middle-aged rats
IS  - 5
VL  - 56
SP  - 904
EP  - 905
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_233
ER  - 
@conference{
author = "Brkić, Dejan and Šošić-Jurjević, Branka  and Filipović, Branko and Ajdžanović, Vladimir and Sekulić, Milka",
year = "2006",
publisher = "Beograd: Udruženje farmaceuta NR Srbije",
journal = "4. kongres farmaceuta Srbije sa međunarodnim učešćem: Savremena farmacija: unapređenje zdravlja i kvaliteta života; 2006 Nov 28 - Dec 2; Belgrade, Serbia",
title = "Genistein i Daidzein snižavaju nivo holesterola u serumu starih orhidektomisanih pacova, Genistein and Daidzein reduces level of total serum cholesterol in orchidectomized middle-aged rats",
number = "5",
volume = "56",
pages = "904-905",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_233"
}
Brkić, D., Šošić-Jurjević, B., Filipović, B., Ajdžanović, V.,& Sekulić, M.. (2006). Genistein i Daidzein snižavaju nivo holesterola u serumu starih orhidektomisanih pacova. in 4. kongres farmaceuta Srbije sa međunarodnim učešćem: Savremena farmacija: unapređenje zdravlja i kvaliteta života; 2006 Nov 28 - Dec 2; Belgrade, Serbia
Beograd: Udruženje farmaceuta NR Srbije., 56(5), 904-905.
https://hdl.handle.net/21.15107/rcub_ibiss_233
Brkić D, Šošić-Jurjević B, Filipović B, Ajdžanović V, Sekulić M. Genistein i Daidzein snižavaju nivo holesterola u serumu starih orhidektomisanih pacova. in 4. kongres farmaceuta Srbije sa međunarodnim učešćem: Savremena farmacija: unapređenje zdravlja i kvaliteta života; 2006 Nov 28 - Dec 2; Belgrade, Serbia. 2006;56(5):904-905.
https://hdl.handle.net/21.15107/rcub_ibiss_233 .
Brkić, Dejan, Šošić-Jurjević, Branka , Filipović, Branko, Ajdžanović, Vladimir, Sekulić, Milka, "Genistein i Daidzein snižavaju nivo holesterola u serumu starih orhidektomisanih pacova" in 4. kongres farmaceuta Srbije sa međunarodnim učešćem: Savremena farmacija: unapređenje zdravlja i kvaliteta života; 2006 Nov 28 - Dec 2; Belgrade, Serbia, 56, no. 5 (2006):904-905,
https://hdl.handle.net/21.15107/rcub_ibiss_233 .

Calcium administration decreases thyroid functioning in middle-aged female rats

Šošić-Jurjević, Branka; Filipović, Branko; Manojlović-Stojanoski, Milica; Sekulić, Milka

(2006)

TY  - JOUR
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
AU  - Manojlović-Stojanoski, Milica
AU  - Sekulić, Milka
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/141
T2  - Archives of Biological Sciences
T1  - Calcium administration decreases thyroid functioning in middle-aged female rats
IS  - 4
VL  - 58
DO  - 10.2298/ABS060431PS
SP  - 31P
EP  - 32P
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_141
ER  - 
@article{
author = "Šošić-Jurjević, Branka and Filipović, Branko and Manojlović-Stojanoski, Milica and Sekulić, Milka",
year = "2006",
journal = "Archives of Biological Sciences",
title = "Calcium administration decreases thyroid functioning in middle-aged female rats",
number = "4",
volume = "58",
doi = "10.2298/ABS060431PS",
pages = "31P-32P",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_141"
}
Šošić-Jurjević, B., Filipović, B., Manojlović-Stojanoski, M.,& Sekulić, M.. (2006). Calcium administration decreases thyroid functioning in middle-aged female rats. in Archives of Biological Sciences, 58(4), 31P-32P.
https://doi.org/10.2298/ABS060431PS
https://hdl.handle.net/21.15107/rcub_ibiss_141
Šošić-Jurjević B, Filipović B, Manojlović-Stojanoski M, Sekulić M. Calcium administration decreases thyroid functioning in middle-aged female rats. in Archives of Biological Sciences. 2006;58(4):31P-32P.
doi:10.2298/ABS060431PS
https://hdl.handle.net/21.15107/rcub_ibiss_141 .
Šošić-Jurjević, Branka, Filipović, Branko, Manojlović-Stojanoski, Milica, Sekulić, Milka, "Calcium administration decreases thyroid functioning in middle-aged female rats" in Archives of Biological Sciences, 58, no. 4 (2006):31P-32P,
https://doi.org/10.2298/ABS060431PS .,
https://hdl.handle.net/21.15107/rcub_ibiss_141 .
1

Chronic somatostatin treatment affects pituitary gonadotrophs, ovaries and onset of puberty in rats

Nestorović, Nataša; Lovren, Mirjana; Sekulić, Milka; Ristić, Nataša; Šošić-Jurjević, Branka ; Filipović, Branko; Milošević, Verica

(Elsevier, 2004)

TY  - JOUR
AU  - Nestorović, Nataša
AU  - Lovren, Mirjana
AU  - Sekulić, Milka
AU  - Ristić, Nataša
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Milošević, Verica
PY  - 2004
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6066
AB  - The effects of chronic somatostatin (SRIH-14) treatment on the pituitary gonadotrophs (FSH and LH cells) and ovaries of female Wistar rats were examined. Females were given 20 microg/100 g b.w. twice per day from the immature (23rd day) till the adult period of life (71st day). The onset of puberty was determined by daily examination for vaginal opening. The peroxidase-antiperoxidase immunocytochemical procedure was used to study the gonadotrophs. Changes in the number per unit area (mm2), cell volume and volume densities of LH- and FSH-immunoreactive cells were evaluated by morphometry and stereology. Ovaries were analysed by simple point counting of follicles and corpora lutea (CL). Follicles were divided by size according to the classification of Gaytán and Osman. The mitotic indexes of granulosa and theca cells in the follicles were estimated at all stages of folliculogenesis. The number, volume and the volume density of FSH- and LH-immunoreactive cells decreased after chronic SRIH-14 treatment, particularly the latter. In the ovary, SRIH-14 treatment decreased the number of healthy follicles at all phases of folliculogenesis, lowered the mitotic indexes of granulosa and theca cells but increased the number of atretic follicles. Healthy CL were fewer in number, while regressive CL were increased. Vaginal opening occurred at a later age in treated females. It can be concluded that chronic SRIH-14 treatment markedly inhibited LH cells and to a lesser extent FSH cells. In the ovary SRIH-14 inhibited folliculogenesis, enhanced atretic processes and lowered proliferative activity of granulosa and theca cells. It also delayed puberty onset.
PB  - Elsevier
T2  - Life Sciences
T1  - Chronic somatostatin treatment affects pituitary gonadotrophs, ovaries and onset of puberty in rats
IS  - 11
VL  - 74
DO  - 10.1016/j.lfs.2003.07.042
SP  - 1359
EP  - 1373
ER  - 
@article{
author = "Nestorović, Nataša and Lovren, Mirjana and Sekulić, Milka and Ristić, Nataša and Šošić-Jurjević, Branka  and Filipović, Branko and Milošević, Verica",
year = "2004",
abstract = "The effects of chronic somatostatin (SRIH-14) treatment on the pituitary gonadotrophs (FSH and LH cells) and ovaries of female Wistar rats were examined. Females were given 20 microg/100 g b.w. twice per day from the immature (23rd day) till the adult period of life (71st day). The onset of puberty was determined by daily examination for vaginal opening. The peroxidase-antiperoxidase immunocytochemical procedure was used to study the gonadotrophs. Changes in the number per unit area (mm2), cell volume and volume densities of LH- and FSH-immunoreactive cells were evaluated by morphometry and stereology. Ovaries were analysed by simple point counting of follicles and corpora lutea (CL). Follicles were divided by size according to the classification of Gaytán and Osman. The mitotic indexes of granulosa and theca cells in the follicles were estimated at all stages of folliculogenesis. The number, volume and the volume density of FSH- and LH-immunoreactive cells decreased after chronic SRIH-14 treatment, particularly the latter. In the ovary, SRIH-14 treatment decreased the number of healthy follicles at all phases of folliculogenesis, lowered the mitotic indexes of granulosa and theca cells but increased the number of atretic follicles. Healthy CL were fewer in number, while regressive CL were increased. Vaginal opening occurred at a later age in treated females. It can be concluded that chronic SRIH-14 treatment markedly inhibited LH cells and to a lesser extent FSH cells. In the ovary SRIH-14 inhibited folliculogenesis, enhanced atretic processes and lowered proliferative activity of granulosa and theca cells. It also delayed puberty onset.",
publisher = "Elsevier",
journal = "Life Sciences",
title = "Chronic somatostatin treatment affects pituitary gonadotrophs, ovaries and onset of puberty in rats",
number = "11",
volume = "74",
doi = "10.1016/j.lfs.2003.07.042",
pages = "1359-1373"
}
Nestorović, N., Lovren, M., Sekulić, M., Ristić, N., Šošić-Jurjević, B., Filipović, B.,& Milošević, V.. (2004). Chronic somatostatin treatment affects pituitary gonadotrophs, ovaries and onset of puberty in rats. in Life Sciences
Elsevier., 74(11), 1359-1373.
https://doi.org/10.1016/j.lfs.2003.07.042
Nestorović N, Lovren M, Sekulić M, Ristić N, Šošić-Jurjević B, Filipović B, Milošević V. Chronic somatostatin treatment affects pituitary gonadotrophs, ovaries and onset of puberty in rats. in Life Sciences. 2004;74(11):1359-1373.
doi:10.1016/j.lfs.2003.07.042 .
Nestorović, Nataša, Lovren, Mirjana, Sekulić, Milka, Ristić, Nataša, Šošić-Jurjević, Branka , Filipović, Branko, Milošević, Verica, "Chronic somatostatin treatment affects pituitary gonadotrophs, ovaries and onset of puberty in rats" in Life Sciences, 74, no. 11 (2004):1359-1373,
https://doi.org/10.1016/j.lfs.2003.07.042 . .
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