TY  - JOUR
AU  - Aleksić, Marija
AU  - Golić, Igor
AU  - Janković, Aleksandra
AU  - Čvoro, Aleksandra
AU  - Korać, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6529
AB  - We previously demonstrated that hypothyroidism increases peroxisomal biogenesis in rat brown adipose tissue (BAT). We also showed heterogeneity in peroxisomal origin and their unique structural association with mitochondria and/or lipid bodies to carry out β-oxidation, contributing thus to BAT thermogenesis. Distinctive heterogeneity creates structural compartmentalization within peroxisomal population, raising the question of whether it is followed by their functional compartmentalization regarding localization/colocalization of two main acyl-CoA oxidase (ACOX) isoforms, ACOX1 and ACOX3. ACOX is the first and rate-limiting enzyme of peroxisomal β-oxidation, and, to date, their protein expression patterns in BAT have not been fully defined. Therefore, we used methimazole-induced hypothyroidism to study ACOX1 and ACOX3 protein expression and their tissue immunolocalization. Additionally, we analysed their specific peroxisomal localization and colocalization in parallel with peroxisomal structural compartmentalization in brown adipocytes. Hypothyroidism caused a linear increase in ACOX1 expression, while a temporary decrease in ACOX3 levels is only recovered to the control level at day 21. Peroxisomal ACOX1 and ACOX3 localization and colocalization patterns entirely mirrored heterogeneous peroxisomal biogenesis pathways and structural compartmentalization, e.g. associations with mitochondria and/or lipid bodies. Hence, different ACOX isoforms localization/colocalization creates distinct functional heterogeneity of peroxisomes and drives their functional compartmentalization in rat brown adipocytes.
PB  - London: Royal society publishing
T2  - Royal Society Open Science
T1  - ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats
VL  - 10
DO  - 10.1098/rsos.230109
SP  - 230109
ER  - 

@article{
author = "Aleksić, Marija and Golić, Igor and Janković, Aleksandra and Čvoro, Aleksandra and Korać, Aleksandra",
year = "2023",
abstract = "We previously demonstrated that hypothyroidism increases peroxisomal biogenesis in rat brown adipose tissue (BAT). We also showed heterogeneity in peroxisomal origin and their unique structural association with mitochondria and/or lipid bodies to carry out β-oxidation, contributing thus to BAT thermogenesis. Distinctive heterogeneity creates structural compartmentalization within peroxisomal population, raising the question of whether it is followed by their functional compartmentalization regarding localization/colocalization of two main acyl-CoA oxidase (ACOX) isoforms, ACOX1 and ACOX3. ACOX is the first and rate-limiting enzyme of peroxisomal β-oxidation, and, to date, their protein expression patterns in BAT have not been fully defined. Therefore, we used methimazole-induced hypothyroidism to study ACOX1 and ACOX3 protein expression and their tissue immunolocalization. Additionally, we analysed their specific peroxisomal localization and colocalization in parallel with peroxisomal structural compartmentalization in brown adipocytes. Hypothyroidism caused a linear increase in ACOX1 expression, while a temporary decrease in ACOX3 levels is only recovered to the control level at day 21. Peroxisomal ACOX1 and ACOX3 localization and colocalization patterns entirely mirrored heterogeneous peroxisomal biogenesis pathways and structural compartmentalization, e.g. associations with mitochondria and/or lipid bodies. Hence, different ACOX isoforms localization/colocalization creates distinct functional heterogeneity of peroxisomes and drives their functional compartmentalization in rat brown adipocytes.",
publisher = "London: Royal society publishing",
journal = "Royal Society Open Science",
title = "ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats",
volume = "10",
doi = "10.1098/rsos.230109",
pages = "230109"
}

Aleksić, M., Golić, I., Janković, A., Čvoro, A.,& Korać, A.. (2023). ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats. in Royal Society Open Science
London: Royal society publishing., 10, 230109.
https://doi.org/10.1098/rsos.230109

Aleksić M, Golić I, Janković A, Čvoro A, Korać A. ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats. in Royal Society Open Science. 2023;10:230109.
doi:10.1098/rsos.230109 .

Aleksić, Marija, Golić, Igor, Janković, Aleksandra, Čvoro, Aleksandra, Korać, Aleksandra, "ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats" in Royal Society Open Science, 10 (2023):230109,
https://doi.org/10.1098/rsos.230109 . .

TY  - JOUR
AU  - Protić, Isidora
AU  - Golić, Igor
AU  - Aleksić, Marija
AU  - Vidaković, Snežana
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0306987722000408
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4862
AB  - The global rise in male infertility necessitates a constant search for underlying causes, diagnostics and corrective treatments. Sperm cells are irreplaceable cells for the process of reproduction. Intrauterine insemination (IUI) is a simple and non-invasive technique, most commonly used to overcome both unexplained and mild male-factor infertility. Even though spermatozoa may be classified as “normal” in terms of morphology, motility and concentration, they could be defective at the subcellular level. Protein lysine acetylation within human sperm, a major post-translational modification of tubulin, is suspected to be a cause of sperm abnormality but is not fully understood. An examination of α-tubulin and acetylated α-tubulin immunopresence, spatial distribution and co-localisation in normozoospermic and asthenozoospermic semen samples was conducted to determine if acetylated α-tubulin could be a biomarker of sperm heterogeneity to better predict sperm fertility potential for IUI outcome. Acetylated α-tubulin was present in both sperm subcompartments, tail and nucleus of normozoospermic samples. We found more immunopositivity for acetylated α-tubulin in the sperm tail and less in nucleus of asthenozoospermic compared to normozoospermic samples. Hence, the acetylated α-tubulin in sperm may contribute to sperm nuclei heterogeneity and serve as a novel molecular biomarker.
PB  - Edinburgh: Churchill Livingstone
T2  - Medical Hypotheses
T1  - Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity
VL  - 161
DO  - 10.1016/j.mehy.2022.110800
SP  - 110800
ER  - 

@article{
author = "Protić, Isidora and Golić, Igor and Aleksić, Marija and Vidaković, Snežana and Korać, Bato and Korać, Aleksandra",
year = "2022",
abstract = "The global rise in male infertility necessitates a constant search for underlying causes, diagnostics and corrective treatments. Sperm cells are irreplaceable cells for the process of reproduction. Intrauterine insemination (IUI) is a simple and non-invasive technique, most commonly used to overcome both unexplained and mild male-factor infertility. Even though spermatozoa may be classified as “normal” in terms of morphology, motility and concentration, they could be defective at the subcellular level. Protein lysine acetylation within human sperm, a major post-translational modification of tubulin, is suspected to be a cause of sperm abnormality but is not fully understood. An examination of α-tubulin and acetylated α-tubulin immunopresence, spatial distribution and co-localisation in normozoospermic and asthenozoospermic semen samples was conducted to determine if acetylated α-tubulin could be a biomarker of sperm heterogeneity to better predict sperm fertility potential for IUI outcome. Acetylated α-tubulin was present in both sperm subcompartments, tail and nucleus of normozoospermic samples. We found more immunopositivity for acetylated α-tubulin in the sperm tail and less in nucleus of asthenozoospermic compared to normozoospermic samples. Hence, the acetylated α-tubulin in sperm may contribute to sperm nuclei heterogeneity and serve as a novel molecular biomarker.",
publisher = "Edinburgh: Churchill Livingstone",
journal = "Medical Hypotheses",
title = "Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity",
volume = "161",
doi = "10.1016/j.mehy.2022.110800",
pages = "110800"
}

Protić, I., Golić, I., Aleksić, M., Vidaković, S., Korać, B.,& Korać, A.. (2022). Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity. in Medical Hypotheses
Edinburgh: Churchill Livingstone., 161, 110800.
https://doi.org/10.1016/j.mehy.2022.110800

Protić I, Golić I, Aleksić M, Vidaković S, Korać B, Korać A. Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity. in Medical Hypotheses. 2022;161:110800.
doi:10.1016/j.mehy.2022.110800 .

Protić, Isidora, Golić, Igor, Aleksić, Marija, Vidaković, Snežana, Korać, Bato, Korać, Aleksandra, "Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity" in Medical Hypotheses, 161 (2022):110800,
https://doi.org/10.1016/j.mehy.2022.110800 . .

TY  - JOUR
AU  - Kalezić, Anđelika
AU  - Udički, Mirjana
AU  - Srdić Galić, Biljana
AU  - Aleksić, Marija
AU  - Korać, Aleksandra
AU  - Janković, Aleksandra
AU  - Korać, Bato
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33765617
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4175
AB  - One of the underlying mechanisms that could link breast cancer and obesity is shifted redox homeostasis in the tumor microenvironment. To reveal the relationship between the malignant phenotype and obesity, we compared redox profiles of breast tumor and tumor-associated adipose tissue from premenopausal women: normal-weight with benign tumors, overweight/obese with benign tumors, normal-weight with malignant tumors, and overweight/obese with malignant tumors. Namely, we examined the protein expression of nuclear factor erythroid 2-related factor 2 (Nrf2), protein expression and activity of main antioxidant defense (AD) enzymes: copper, zinc- and manganese superoxide dismutase, catalase, and glutathione peroxidase, as well as the level of 4-hydroxy-2-nonenal (4-HNE) modified proteins. Higher protein expression and activity of AD enzymes were found in malignant tumor tissue than benign tumor tissue, irrespective of obesity. Nevertheless, malignant tumor tissue of overweight/obese women was characterized by higher protein expression of Nrf2 and weaker immunopositivity for 4-HNE modified proteins. In malignant tumor-associated adipose tissue, the redox profile was clearly related to obesity. Higher Nrf2 protein expression and higher AD enzyme levels were observed in normal-weight women, while stronger immunopositivity for 4-HNE modified proteins was found in overweight/obese women. The results suggest that the complex interplay between obesity and malignancy involves redox-sensitive pathways in breast tumor and tumor-associated adipose tissue.
PB  - Elsevier BV
T2  - Redox Biology
T1  - Redox profile of breast tumor and associated adipose tissue in premenopausal women - Interplay between obesity and malignancy.
VL  - 41
DO  - 10.1016/j.redox.2021.101939
SP  - 101939
ER  - 

@article{
author = "Kalezić, Anđelika and Udički, Mirjana and Srdić Galić, Biljana and Aleksić, Marija and Korać, Aleksandra and Janković, Aleksandra and Korać, Bato",
year = "2021",
abstract = "One of the underlying mechanisms that could link breast cancer and obesity is shifted redox homeostasis in the tumor microenvironment. To reveal the relationship between the malignant phenotype and obesity, we compared redox profiles of breast tumor and tumor-associated adipose tissue from premenopausal women: normal-weight with benign tumors, overweight/obese with benign tumors, normal-weight with malignant tumors, and overweight/obese with malignant tumors. Namely, we examined the protein expression of nuclear factor erythroid 2-related factor 2 (Nrf2), protein expression and activity of main antioxidant defense (AD) enzymes: copper, zinc- and manganese superoxide dismutase, catalase, and glutathione peroxidase, as well as the level of 4-hydroxy-2-nonenal (4-HNE) modified proteins. Higher protein expression and activity of AD enzymes were found in malignant tumor tissue than benign tumor tissue, irrespective of obesity. Nevertheless, malignant tumor tissue of overweight/obese women was characterized by higher protein expression of Nrf2 and weaker immunopositivity for 4-HNE modified proteins. In malignant tumor-associated adipose tissue, the redox profile was clearly related to obesity. Higher Nrf2 protein expression and higher AD enzyme levels were observed in normal-weight women, while stronger immunopositivity for 4-HNE modified proteins was found in overweight/obese women. The results suggest that the complex interplay between obesity and malignancy involves redox-sensitive pathways in breast tumor and tumor-associated adipose tissue.",
publisher = "Elsevier BV",
journal = "Redox Biology",
title = "Redox profile of breast tumor and associated adipose tissue in premenopausal women - Interplay between obesity and malignancy.",
volume = "41",
doi = "10.1016/j.redox.2021.101939",
pages = "101939"
}

Kalezić, A., Udički, M., Srdić Galić, B., Aleksić, M., Korać, A., Janković, A.,& Korać, B.. (2021). Redox profile of breast tumor and associated adipose tissue in premenopausal women - Interplay between obesity and malignancy.. in Redox Biology
Elsevier BV., 41, 101939.
https://doi.org/10.1016/j.redox.2021.101939

Kalezić A, Udički M, Srdić Galić B, Aleksić M, Korać A, Janković A, Korać B. Redox profile of breast tumor and associated adipose tissue in premenopausal women - Interplay between obesity and malignancy.. in Redox Biology. 2021;41:101939.
doi:10.1016/j.redox.2021.101939 .

Kalezić, Anđelika, Udički, Mirjana, Srdić Galić, Biljana, Aleksić, Marija, Korać, Aleksandra, Janković, Aleksandra, Korać, Bato, "Redox profile of breast tumor and associated adipose tissue in premenopausal women - Interplay between obesity and malignancy." in Redox Biology, 41 (2021):101939,
https://doi.org/10.1016/j.redox.2021.101939 . .

TY  - JOUR
AU  - Aleksić, Marija
AU  - Kalezić, Anđelika
AU  - Saso, Luciano
AU  - Janković, Aleksandra
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4216
AB  - Brown adipose tissue (BAT) is important for maintaining whole-body metabolic and energy homeostasis. However, the effects of hypothyroidism, one of the most common diseases worldwide, which increases the risk of several metabolic disorders, on BAT redox and metabolic homeostasis remain mostly unknown. We aimed to investigate the dynamics of protein expression, enzyme activity, and localization of antioxidant defense (AD) enzymes in rat interscapular BAT upon induction of hypothyroidism by antithyroid drug methimazole for 7, 15, and 21 days. Our results showed an increased protein expression of CuZn-and Mn-superoxide dismutase, catalase, glutamyl– cysteine ligase, thioredoxin, total glutathione content, and activity of catalase and thioredoxin reductase in hypothyroid rats, compared to euthyroid control. Concomitant with the increase in AD, newly established nuclear, mitochondrial, and peroxisomal localization of AD enzymes was found. Hypothyroidism also potentiated associations between mitochondria, peroxisomes, and lipid bodies, creating specific structural–functional units. Moreover, hypothyroidism induced protein expression and nuclear translocation of a master regulator of redox-metabolic homeostasis, nuclear factor erythroid 2-related factor 2 (Nrf2), and an increased amount of 4-hydroxynonenal (4-HNE) protein adducts. The results indicate that spatiotemporal overlap in the remodeling of AD is orchestrated by Nrf2, implicating the role of 4-HNE in this process and suggesting the potential mechanism of redox-structural remodeling during BAT adaptation in hypothyroidism.
PB  - MDPI AG
T2  - Antioxidants
T1  - The unity of redox and structural remodeling of brown adipose tissue in hypothyroidism
IS  - 4
VL  - 10
DO  - 10.3390/antiox10040591
SP  - 591
ER  - 

@article{
author = "Aleksić, Marija and Kalezić, Anđelika and Saso, Luciano and Janković, Aleksandra and Korać, Bato and Korać, Aleksandra",
year = "2021",
abstract = "Brown adipose tissue (BAT) is important for maintaining whole-body metabolic and energy homeostasis. However, the effects of hypothyroidism, one of the most common diseases worldwide, which increases the risk of several metabolic disorders, on BAT redox and metabolic homeostasis remain mostly unknown. We aimed to investigate the dynamics of protein expression, enzyme activity, and localization of antioxidant defense (AD) enzymes in rat interscapular BAT upon induction of hypothyroidism by antithyroid drug methimazole for 7, 15, and 21 days. Our results showed an increased protein expression of CuZn-and Mn-superoxide dismutase, catalase, glutamyl– cysteine ligase, thioredoxin, total glutathione content, and activity of catalase and thioredoxin reductase in hypothyroid rats, compared to euthyroid control. Concomitant with the increase in AD, newly established nuclear, mitochondrial, and peroxisomal localization of AD enzymes was found. Hypothyroidism also potentiated associations between mitochondria, peroxisomes, and lipid bodies, creating specific structural–functional units. Moreover, hypothyroidism induced protein expression and nuclear translocation of a master regulator of redox-metabolic homeostasis, nuclear factor erythroid 2-related factor 2 (Nrf2), and an increased amount of 4-hydroxynonenal (4-HNE) protein adducts. The results indicate that spatiotemporal overlap in the remodeling of AD is orchestrated by Nrf2, implicating the role of 4-HNE in this process and suggesting the potential mechanism of redox-structural remodeling during BAT adaptation in hypothyroidism.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "The unity of redox and structural remodeling of brown adipose tissue in hypothyroidism",
number = "4",
volume = "10",
doi = "10.3390/antiox10040591",
pages = "591"
}

Aleksić, M., Kalezić, A., Saso, L., Janković, A., Korać, B.,& Korać, A.. (2021). The unity of redox and structural remodeling of brown adipose tissue in hypothyroidism. in Antioxidants
MDPI AG., 10(4), 591.
https://doi.org/10.3390/antiox10040591

Aleksić M, Kalezić A, Saso L, Janković A, Korać B, Korać A. The unity of redox and structural remodeling of brown adipose tissue in hypothyroidism. in Antioxidants. 2021;10(4):591.
doi:10.3390/antiox10040591 .

Aleksić, Marija, Kalezić, Anđelika, Saso, Luciano, Janković, Aleksandra, Korać, Bato, Korać, Aleksandra, "The unity of redox and structural remodeling of brown adipose tissue in hypothyroidism" in Antioxidants, 10, no. 4 (2021):591,
https://doi.org/10.3390/antiox10040591 . .

TY  - JOUR
AU  - Kalezić, Anđelika
AU  - Udički, Mirjana
AU  - Srdić Galić, Biljana
AU  - Aleksić, Marija
AU  - Korać, Aleksandra
AU  - Janković, Aleksandra
AU  - Korać, Bato
PY  - 2021
UR  - https://www.mdpi.com/2072-6694/13/11/2731
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4407
AB  - Typical features of the breast malignant phenotype rely on metabolic reprogramming of cancer cells and their interaction with surrounding adipocytes. Obesity is strongly associated with breast cancer mortality, yet the effects of obesity on metabolic reprogramming of cancer and cancer-associated adipose tissue remain largely unknown. Paired biopsies of breast tumor tissue and adipose tissue from premenopausal women were divided according to pathohistological analyses and body mass index on normal-weight and overweight/obese with benign or malignant tumors. We investigated the protein expression of key regulatory enzymes of glycolysis, pentose phosphate pathway (PPP), and glycogen synthesis. Breast cancer tissue showed a simultaneous increase in 5′-AMP-activated protein kinase (AMPK) protein expression with typical features of the Warburg effect, including hexokinase 2 (HK 2) overexpression and its association with mitochondrial voltage-dependent anion-selective channel protein 1, associated with an overexpression of rate-limiting enzymes of glycolysis (phosphofructokinase 1—PFK-1) and pentose phosphate pathway (glucose-6-phosphate dehydrogenase—G6PDH). In parallel, cancer-associated adipose tissue showed increased AMPK protein expression with overexpression of HK 2 and G6PDH in line with increased PPP activity. Moreover, important obesity-associated differences in glucose metabolism were observed in breast cancer tissue showing prominent glycogen deposition and higher glycogen synthase kinase-3 protein expression in normal-weight women and higher PFK-1 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein expression in overweight/obese women. In conclusion, metabolic reprogramming of glycolysis contributes to tissue-specific Warburg effect in breast cancer and cancer-associated adipose tissue.
T2  - Cancers
T1  - Tissue-Specific Warburg Effect in Breast Cancer and Cancer-Associated Adipose Tissue—Relationship between AMPK and Glycolysis
IS  - 11
VL  - 13
DO  - 10.3390/cancers13112731
SP  - 2731
ER  - 

@article{
author = "Kalezić, Anđelika and Udički, Mirjana and Srdić Galić, Biljana and Aleksić, Marija and Korać, Aleksandra and Janković, Aleksandra and Korać, Bato",
year = "2021",
abstract = "Typical features of the breast malignant phenotype rely on metabolic reprogramming of cancer cells and their interaction with surrounding adipocytes. Obesity is strongly associated with breast cancer mortality, yet the effects of obesity on metabolic reprogramming of cancer and cancer-associated adipose tissue remain largely unknown. Paired biopsies of breast tumor tissue and adipose tissue from premenopausal women were divided according to pathohistological analyses and body mass index on normal-weight and overweight/obese with benign or malignant tumors. We investigated the protein expression of key regulatory enzymes of glycolysis, pentose phosphate pathway (PPP), and glycogen synthesis. Breast cancer tissue showed a simultaneous increase in 5′-AMP-activated protein kinase (AMPK) protein expression with typical features of the Warburg effect, including hexokinase 2 (HK 2) overexpression and its association with mitochondrial voltage-dependent anion-selective channel protein 1, associated with an overexpression of rate-limiting enzymes of glycolysis (phosphofructokinase 1—PFK-1) and pentose phosphate pathway (glucose-6-phosphate dehydrogenase—G6PDH). In parallel, cancer-associated adipose tissue showed increased AMPK protein expression with overexpression of HK 2 and G6PDH in line with increased PPP activity. Moreover, important obesity-associated differences in glucose metabolism were observed in breast cancer tissue showing prominent glycogen deposition and higher glycogen synthase kinase-3 protein expression in normal-weight women and higher PFK-1 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein expression in overweight/obese women. In conclusion, metabolic reprogramming of glycolysis contributes to tissue-specific Warburg effect in breast cancer and cancer-associated adipose tissue.",
journal = "Cancers",
title = "Tissue-Specific Warburg Effect in Breast Cancer and Cancer-Associated Adipose Tissue—Relationship between AMPK and Glycolysis",
number = "11",
volume = "13",
doi = "10.3390/cancers13112731",
pages = "2731"
}

Kalezić, A., Udički, M., Srdić Galić, B., Aleksić, M., Korać, A., Janković, A.,& Korać, B.. (2021). Tissue-Specific Warburg Effect in Breast Cancer and Cancer-Associated Adipose Tissue—Relationship between AMPK and Glycolysis. in Cancers, 13(11), 2731.
https://doi.org/10.3390/cancers13112731

Kalezić A, Udički M, Srdić Galić B, Aleksić M, Korać A, Janković A, Korać B. Tissue-Specific Warburg Effect in Breast Cancer and Cancer-Associated Adipose Tissue—Relationship between AMPK and Glycolysis. in Cancers. 2021;13(11):2731.
doi:10.3390/cancers13112731 .

Kalezić, Anđelika, Udički, Mirjana, Srdić Galić, Biljana, Aleksić, Marija, Korać, Aleksandra, Janković, Aleksandra, Korać, Bato, "Tissue-Specific Warburg Effect in Breast Cancer and Cancer-Associated Adipose Tissue—Relationship between AMPK and Glycolysis" in Cancers, 13, no. 11 (2021):2731,
https://doi.org/10.3390/cancers13112731 . .

TY  - JOUR
AU  - Aleksić, Marija
AU  - Golić, Igor
AU  - Kalezić, Anđelika
AU  - Janković, Aleksandra
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2021
UR  - https://www.mdpi.com/2073-4409/10/9/2248
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8472630
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4491
AB  - Despite peroxisomes being important partners of mitochondria by carrying out fatty acid oxidation in brown adipocytes, no clear evidence concerning peroxisome origin and way(s) of biogenesis exists. Herein we used methimazole-induced hypothyroidism for 7, 15, and 21 days to study peroxisomal remodeling and origin in rat brown adipocytes. We found that peroxisomes originated via both canonic, and de novo pathways. Each pathway operates in euthyroid control and over the course of hypothyroidism, in a time-dependent manner. Hypothyroidism increased the peroxisomal number by 1.8-, 3.6- and 5.8-fold on days 7, 15, and 21. Peroxisomal presence, their distribution, and their degree of maturation were heterogeneous in brown adipocytes in a Harlequin-like manner, reflecting differences in their origin. The canonic pathway, through numerous dumbbell-like and "pearls on strings" structures, supported by high levels of Pex11β and Drp1, prevailed on day 7. The de novo pathway of peroxisomal biogenesis started on day 15 and became dominant by day 21. The transition of peroxisomal biogenesis from canonic to the de novo pathway was driven by increased levels of Pex19, PMP70, Pex5S, and Pex26 and characterized by numerous tubular structures. Furthermore, specific peroxisomal origin from mitochondria, regardless of thyroid status, indicates their mutual regulation in rat brown adipocytes.
PB  - Basel: MDPI
T2  - Cells
T1  - Hypothyroidism Intensifies Both Canonic and the De Novo Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner.
IS  - 9
VL  - 10
DO  - 10.3390/cells10092248
SP  - 2248
ER  - 

@article{
author = "Aleksić, Marija and Golić, Igor and Kalezić, Anđelika and Janković, Aleksandra and Korać, Bato and Korać, Aleksandra",
year = "2021",
abstract = "Despite peroxisomes being important partners of mitochondria by carrying out fatty acid oxidation in brown adipocytes, no clear evidence concerning peroxisome origin and way(s) of biogenesis exists. Herein we used methimazole-induced hypothyroidism for 7, 15, and 21 days to study peroxisomal remodeling and origin in rat brown adipocytes. We found that peroxisomes originated via both canonic, and de novo pathways. Each pathway operates in euthyroid control and over the course of hypothyroidism, in a time-dependent manner. Hypothyroidism increased the peroxisomal number by 1.8-, 3.6- and 5.8-fold on days 7, 15, and 21. Peroxisomal presence, their distribution, and their degree of maturation were heterogeneous in brown adipocytes in a Harlequin-like manner, reflecting differences in their origin. The canonic pathway, through numerous dumbbell-like and "pearls on strings" structures, supported by high levels of Pex11β and Drp1, prevailed on day 7. The de novo pathway of peroxisomal biogenesis started on day 15 and became dominant by day 21. The transition of peroxisomal biogenesis from canonic to the de novo pathway was driven by increased levels of Pex19, PMP70, Pex5S, and Pex26 and characterized by numerous tubular structures. Furthermore, specific peroxisomal origin from mitochondria, regardless of thyroid status, indicates their mutual regulation in rat brown adipocytes.",
publisher = "Basel: MDPI",
journal = "Cells",
title = "Hypothyroidism Intensifies Both Canonic and the De Novo Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner.",
number = "9",
volume = "10",
doi = "10.3390/cells10092248",
pages = "2248"
}

Aleksić, M., Golić, I., Kalezić, A., Janković, A., Korać, B.,& Korać, A.. (2021). Hypothyroidism Intensifies Both Canonic and the De Novo Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner.. in Cells
Basel: MDPI., 10(9), 2248.
https://doi.org/10.3390/cells10092248

Aleksić M, Golić I, Kalezić A, Janković A, Korać B, Korać A. Hypothyroidism Intensifies Both Canonic and the De Novo Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner.. in Cells. 2021;10(9):2248.
doi:10.3390/cells10092248 .

Aleksić, Marija, Golić, Igor, Kalezić, Anđelika, Janković, Aleksandra, Korać, Bato, Korać, Aleksandra, "Hypothyroidism Intensifies Both Canonic and the De Novo Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner." in Cells, 10, no. 9 (2021):2248,
https://doi.org/10.3390/cells10092248 . .

TY  - CONF
AU  - Golić, Igor
AU  - Aleksić, Marija
AU  - Kalezić, Anđelika
AU  - Janković, Aleksandra
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4803
AB  - The disequilibrium of reactive oxygen/nitrogen species (ROS/RNS) is one of the causes of male infertility. To examine the role of ROS/RNS in reproduction, we modulated the cell concentration of О2•– and NO using the superoxide dismutase (SOD) mimic, M40403, which selectively removes О2•– and consequently increases the NO bioavailability. The fine chromatin changes are difficult to observe via routine light/electron microscopy, therefore we used fractal analysis to analyze DNA compaction. We used normospermic semen samples from ten human subjects. After purification in Cook density gradient, the sperm-rich fraction was rinsed in modified Tyrod medium (TM). Purified samples were divided into three groups. One group was evaluated immediately after resuspension in TM and served as the control. For the other two groups, untreated and SOD mimic-treated group (50 μM), the sperm was resuspended in TM and evaluated after incubation at 37 ºC / 6% CO2 for 3 hours. Air-dried and methanol fixed sperm smears were stained with toluidine blue and analyzed on Leica DMLB microscope. Images from ten randomly selected fields were analyzed in ImageJ plugin FracLac to get fractal dimension defined as a measure of complexity. Average values of fractal dimensions (mean ± SEM) are: control group – 1.016 ± 0.553; untreated group – 0.955 ± 0.291; treated group – 1.146 ± 0.096. Also, average values of lacunarity are: control group – 0.734 ± 0.129; untreated group – 0.727 ± 0.129; treated group – 0.901 ± 0.297. Results show that SOD mimic remodels chromatin condensation by increasing the euchromatin area, potentially leading to a resume in transcriptional activity. This hypothesis would be consistent with our published findings of up-regulated mRNA expression of eNOS, MnSOD, and catalase in SOD mimic-treated spermatozoa ex vivo (Otasevic et al., 2013). Therefore, SOD mimic modulates the redox environment via direct chromatin remodeling in spermatozoa. Fractal analysis has proven to be a good method for the analysis of chromatin condensation in human sperm nuclei. Ref: Otasevic V, Korac A, Vucetic M, Macanovic B, Garalejic E, Ivanovic-Burmazovic I, Filipovic MR, Buzadzic B, Stancic A, Jankovic A, Velickovic K, Golic I, Markelic M, Korac B. Is manganese (II) pentaazamacrocyclic superoxide dismutase mimic beneficial for human sperm mitochondria function and motility? Antioxid Redox Signal. 2013 Jan 10;18(2):170-8. doi: 10.1089/ars.2012.4684. Epub 2012 Jun 12. PMID: 22563824; PMCID: PMC3513981
PB  - Elsevier Inc.
C3  - Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia
T1  - Fractal analysis of chromatin condensation in the human sperm nuclei
DO  - 10.1016/j.freeradbiomed.2021.08.178
SP  - S114
ER  - 

@conference{
author = "Golić, Igor and Aleksić, Marija and Kalezić, Anđelika and Janković, Aleksandra and Korać, Bato and Korać, Aleksandra",
year = "2021",
abstract = "The disequilibrium of reactive oxygen/nitrogen species (ROS/RNS) is one of the causes of male infertility. To examine the role of ROS/RNS in reproduction, we modulated the cell concentration of О2•– and NO using the superoxide dismutase (SOD) mimic, M40403, which selectively removes О2•– and consequently increases the NO bioavailability. The fine chromatin changes are difficult to observe via routine light/electron microscopy, therefore we used fractal analysis to analyze DNA compaction. We used normospermic semen samples from ten human subjects. After purification in Cook density gradient, the sperm-rich fraction was rinsed in modified Tyrod medium (TM). Purified samples were divided into three groups. One group was evaluated immediately after resuspension in TM and served as the control. For the other two groups, untreated and SOD mimic-treated group (50 μM), the sperm was resuspended in TM and evaluated after incubation at 37 ºC / 6% CO2 for 3 hours. Air-dried and methanol fixed sperm smears were stained with toluidine blue and analyzed on Leica DMLB microscope. Images from ten randomly selected fields were analyzed in ImageJ plugin FracLac to get fractal dimension defined as a measure of complexity. Average values of fractal dimensions (mean ± SEM) are: control group – 1.016 ± 0.553; untreated group – 0.955 ± 0.291; treated group – 1.146 ± 0.096. Also, average values of lacunarity are: control group – 0.734 ± 0.129; untreated group – 0.727 ± 0.129; treated group – 0.901 ± 0.297. Results show that SOD mimic remodels chromatin condensation by increasing the euchromatin area, potentially leading to a resume in transcriptional activity. This hypothesis would be consistent with our published findings of up-regulated mRNA expression of eNOS, MnSOD, and catalase in SOD mimic-treated spermatozoa ex vivo (Otasevic et al., 2013). Therefore, SOD mimic modulates the redox environment via direct chromatin remodeling in spermatozoa. Fractal analysis has proven to be a good method for the analysis of chromatin condensation in human sperm nuclei. Ref: Otasevic V, Korac A, Vucetic M, Macanovic B, Garalejic E, Ivanovic-Burmazovic I, Filipovic MR, Buzadzic B, Stancic A, Jankovic A, Velickovic K, Golic I, Markelic M, Korac B. Is manganese (II) pentaazamacrocyclic superoxide dismutase mimic beneficial for human sperm mitochondria function and motility? Antioxid Redox Signal. 2013 Jan 10;18(2):170-8. doi: 10.1089/ars.2012.4684. Epub 2012 Jun 12. PMID: 22563824; PMCID: PMC3513981",
publisher = "Elsevier Inc.",
journal = "Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia",
title = "Fractal analysis of chromatin condensation in the human sperm nuclei",
doi = "10.1016/j.freeradbiomed.2021.08.178",
pages = "S114"
}

Golić, I., Aleksić, M., Kalezić, A., Janković, A., Korać, B.,& Korać, A.. (2021). Fractal analysis of chromatin condensation in the human sperm nuclei. in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia
Elsevier Inc.., S114.
https://doi.org/10.1016/j.freeradbiomed.2021.08.178

Golić I, Aleksić M, Kalezić A, Janković A, Korać B, Korać A. Fractal analysis of chromatin condensation in the human sperm nuclei. in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia. 2021;:S114.
doi:10.1016/j.freeradbiomed.2021.08.178 .

Golić, Igor, Aleksić, Marija, Kalezić, Anđelika, Janković, Aleksandra, Korać, Bato, Korać, Aleksandra, "Fractal analysis of chromatin condensation in the human sperm nuclei" in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia (2021):S114,
https://doi.org/10.1016/j.freeradbiomed.2021.08.178 . .

TY  - JOUR
AU  - Miler, Irena
AU  - Rabasović, Mihailo D.
AU  - Aleksić, Marija
AU  - Krmpot, Aleksandar J.
AU  - Kalezić, Anđelika
AU  - Janković, Aleksandra
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/10.1002/jbio.202000362
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4072
AB  - Our previous study on rat skin showed that cumulative oxidative pressure induces profound structural and ultrastructural alterations in both rat skin epidermis and dermis during aging. Here, we aimed to investigate the biophotonic properties of collagen as a main dermal component in the function of chronological aging. We used second harmonic generation (SHG) and two‐photon excited fluorescence (TPEF) on 5 μm thick skin paraffin sections from 15‐day‐, 1‐month‐ and 21‐month‐old rats, respectively, to analyze collagen alterations, in comparison to conventional light and electron microscopy methods. Obtained results show that polarization‐resolved SHG (PSHG) images can detect collagen fiber alterations in line with chronological aging and that this method is consistent with light and electron microscopy. Moreover, the β coefficient calculated from PSHG images points out that delicate alterations lead to a more ordered structure of collagen molecules due to oxidative damage. The results of this study also open the possibility of successfully applying this fast and label‐free method to previously fixed samples.image
PB  - Wiley
T2  - Journal of Biophotonics
T1  - Polarization‐resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy
DO  - 10.1002/jbio.202000362
ER  - 

@article{
author = "Miler, Irena and Rabasović, Mihailo D. and Aleksić, Marija and Krmpot, Aleksandar J. and Kalezić, Anđelika and Janković, Aleksandra and Korać, Bato and Korać, Aleksandra",
year = "2020",
abstract = "Our previous study on rat skin showed that cumulative oxidative pressure induces profound structural and ultrastructural alterations in both rat skin epidermis and dermis during aging. Here, we aimed to investigate the biophotonic properties of collagen as a main dermal component in the function of chronological aging. We used second harmonic generation (SHG) and two‐photon excited fluorescence (TPEF) on 5 μm thick skin paraffin sections from 15‐day‐, 1‐month‐ and 21‐month‐old rats, respectively, to analyze collagen alterations, in comparison to conventional light and electron microscopy methods. Obtained results show that polarization‐resolved SHG (PSHG) images can detect collagen fiber alterations in line with chronological aging and that this method is consistent with light and electron microscopy. Moreover, the β coefficient calculated from PSHG images points out that delicate alterations lead to a more ordered structure of collagen molecules due to oxidative damage. The results of this study also open the possibility of successfully applying this fast and label‐free method to previously fixed samples.image",
publisher = "Wiley",
journal = "Journal of Biophotonics",
title = "Polarization‐resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy",
doi = "10.1002/jbio.202000362"
}

Miler, I., Rabasović, M. D., Aleksić, M., Krmpot, A. J., Kalezić, A., Janković, A., Korać, B.,& Korać, A.. (2020). Polarization‐resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy. in Journal of Biophotonics
Wiley..
https://doi.org/10.1002/jbio.202000362

Miler I, Rabasović MD, Aleksić M, Krmpot AJ, Kalezić A, Janković A, Korać B, Korać A. Polarization‐resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy. in Journal of Biophotonics. 2020;.
doi:10.1002/jbio.202000362 .

Miler, Irena, Rabasović, Mihailo D., Aleksić, Marija, Krmpot, Aleksandar J., Kalezić, Anđelika, Janković, Aleksandra, Korać, Bato, Korać, Aleksandra, "Polarization‐resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy" in Journal of Biophotonics (2020),
https://doi.org/10.1002/jbio.202000362 . .

TY  - JOUR
AU  - Kalezić, Anđelika
AU  - Udicki, Mirjana
AU  - Srdić Galić, Biljana
AU  - Aleksić, Marija
AU  - Korać, Aleksandra
AU  - Janković, Aleksandra
AU  - Korać, Bato
PY  - 2020
UR  - https://www.mdpi.com/1422-0067/21/24/9676
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4082
AB  - Metabolic reprogramming that favors high glycolytic flux with lactate production in normoxia is among cancer hallmarks. Lactate is an essential oncometabolite regulating cellular redox homeostasis, energy substrate partitioning, and intracellular signaling. Moreover, malignant phenotype’s chief characteristics are dependent on the interaction between cancer cells and their microenvironment. In breast cancer, mammary adipocytes represent an essential cellular component of the tumor milieu. We analyzed lactate concentration, lactate dehydrogenase (LDH) activity, and isozyme pattern, and LDHA/LDHB protein expression and tissue localization in paired biopsies of breast cancer tissue and cancer-associated adipose tissue in normal-weight and overweight/obese premenopausal women, compared to benign breast tumor tissue and adipose tissue in normal-weight and overweight/obese premenopausal women. We show that higher lactate concentration in cancer tissue is concomitant with a shift in isozyme pattern towards the “muscle-type” LDH and corresponding LDHA and LDHB protein expression changes. In contrast, significantly higher LDH activity in cancer-associated adipose tissue seems to be directed towards lactate oxidation. Moreover, localization patterns of LDH isoforms varied substantially across different areas of breast cancer tissue. Invasive front of the tumor showed cell-specific protein localization of LDHA in breast cancer cells and LDHB in cancer-associated adipocytes. The results suggest a specific, lactate-centric relationship between cancer tissue and cancer-associated adipose tissue and indicate how cancer-adipose tissue cross-talk may be influenced by obesity in premenopausal women.
PB  - MDPI AG
T2  - International Journal of Molecular Sciences
T1  - Lactate Metabolism in Breast Cancer Microenvironment: Contribution Focused on Associated Adipose Tissue and Obesity
IS  - 24
VL  - 21
DO  - 10.3390/ijms21249676
SP  - 9676
ER  - 

@article{
author = "Kalezić, Anđelika and Udicki, Mirjana and Srdić Galić, Biljana and Aleksić, Marija and Korać, Aleksandra and Janković, Aleksandra and Korać, Bato",
year = "2020",
abstract = "Metabolic reprogramming that favors high glycolytic flux with lactate production in normoxia is among cancer hallmarks. Lactate is an essential oncometabolite regulating cellular redox homeostasis, energy substrate partitioning, and intracellular signaling. Moreover, malignant phenotype’s chief characteristics are dependent on the interaction between cancer cells and their microenvironment. In breast cancer, mammary adipocytes represent an essential cellular component of the tumor milieu. We analyzed lactate concentration, lactate dehydrogenase (LDH) activity, and isozyme pattern, and LDHA/LDHB protein expression and tissue localization in paired biopsies of breast cancer tissue and cancer-associated adipose tissue in normal-weight and overweight/obese premenopausal women, compared to benign breast tumor tissue and adipose tissue in normal-weight and overweight/obese premenopausal women. We show that higher lactate concentration in cancer tissue is concomitant with a shift in isozyme pattern towards the “muscle-type” LDH and corresponding LDHA and LDHB protein expression changes. In contrast, significantly higher LDH activity in cancer-associated adipose tissue seems to be directed towards lactate oxidation. Moreover, localization patterns of LDH isoforms varied substantially across different areas of breast cancer tissue. Invasive front of the tumor showed cell-specific protein localization of LDHA in breast cancer cells and LDHB in cancer-associated adipocytes. The results suggest a specific, lactate-centric relationship between cancer tissue and cancer-associated adipose tissue and indicate how cancer-adipose tissue cross-talk may be influenced by obesity in premenopausal women.",
publisher = "MDPI AG",
journal = "International Journal of Molecular Sciences",
title = "Lactate Metabolism in Breast Cancer Microenvironment: Contribution Focused on Associated Adipose Tissue and Obesity",
number = "24",
volume = "21",
doi = "10.3390/ijms21249676",
pages = "9676"
}

Kalezić, A., Udicki, M., Srdić Galić, B., Aleksić, M., Korać, A., Janković, A.,& Korać, B.. (2020). Lactate Metabolism in Breast Cancer Microenvironment: Contribution Focused on Associated Adipose Tissue and Obesity. in International Journal of Molecular Sciences
MDPI AG., 21(24), 9676.
https://doi.org/10.3390/ijms21249676

Kalezić A, Udicki M, Srdić Galić B, Aleksić M, Korać A, Janković A, Korać B. Lactate Metabolism in Breast Cancer Microenvironment: Contribution Focused on Associated Adipose Tissue and Obesity. in International Journal of Molecular Sciences. 2020;21(24):9676.
doi:10.3390/ijms21249676 .

Kalezić, Anđelika, Udicki, Mirjana, Srdić Galić, Biljana, Aleksić, Marija, Korać, Aleksandra, Janković, Aleksandra, Korać, Bato, "Lactate Metabolism in Breast Cancer Microenvironment: Contribution Focused on Associated Adipose Tissue and Obesity" in International Journal of Molecular Sciences, 21, no. 24 (2020):9676,
https://doi.org/10.3390/ijms21249676 . .