TY  - JOUR
AU  - Bensing, Christian
AU  - Mojić, Marija
AU  - Bulatović, Mirna
AU  - Edeler, David
AU  - Pérez-Quintanilla, Damian
AU  - Gómez-Ruiz, Santiago
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Kaluđerović, Goran N.
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2772950822003314
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5106
AB  - A series of nanostructured SBA-15-based materials functionalized with the tetraorganotin(IV) metallodrugs Ph3Sn(CH2)nOH (n = 3, 4, 6, 8 and 11) are synthesized and structurally characterized by different techniques used in solid-state chemistry. The cytotoxicity of both the organotin(IV) compounds and the tin-functionalized SBA-15 materials are studied against different cancer cell lines observing that the materials have similar cytotoxic activity in comparison with the free organotin compounds in terms of mass. However, considering that the percentage of active metal compound loaded into material is low, the utilization of mesoporous silica as drug vehicle clearly improves the cytotoxic effectiveness of metal-based drugs against cancer cells. One of the most potent between all tested systems is material SBA-15~Cl|Ph3Sn(CH2)8OH. Its cytotoxicity seems to come from additional mechanisms apart from apoptosis provoking cell reprogram in B16 melanoma into more mature and less aggressive phenotype. Moderated production of ROS/RNS is probably in the background of observed phenomenon. Obtained results are further confirmed in syngeneic mouse model of melanoma in C57BL6 mice. The in vivo results show that SBA-15 do not disturb tumor growth, while both Ph3Sn(CH2)8OH and SBA-15~Cl|Ph3Sn(CH2)8OH significantly decreases tumor volume with an enhancement of the antitumor potential of the tetraorganotin(IV) compound upon immobilization in SBA-15.
PB  - Elsevier B.V.
T2  - Biomaterials Advances
T1  - Effect of chain length on the cytotoxic activity of (alkyl-ω-ol)triphenyltin(IV) loaded into SBA-15 nanostructured silica and in vivo study of SBA-15~Cl|Ph3Sn(CH2)8OH.
VL  - 140
DO  - 10.1016/j.bioadv.2022.213054
SP  - 213054
ER  - 

@article{
author = "Bensing, Christian and Mojić, Marija and Bulatović, Mirna and Edeler, David and Pérez-Quintanilla, Damian and Gómez-Ruiz, Santiago and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Kaluđerović, Goran N.",
year = "2022",
abstract = "A series of nanostructured SBA-15-based materials functionalized with the tetraorganotin(IV) metallodrugs Ph3Sn(CH2)nOH (n = 3, 4, 6, 8 and 11) are synthesized and structurally characterized by different techniques used in solid-state chemistry. The cytotoxicity of both the organotin(IV) compounds and the tin-functionalized SBA-15 materials are studied against different cancer cell lines observing that the materials have similar cytotoxic activity in comparison with the free organotin compounds in terms of mass. However, considering that the percentage of active metal compound loaded into material is low, the utilization of mesoporous silica as drug vehicle clearly improves the cytotoxic effectiveness of metal-based drugs against cancer cells. One of the most potent between all tested systems is material SBA-15~Cl|Ph3Sn(CH2)8OH. Its cytotoxicity seems to come from additional mechanisms apart from apoptosis provoking cell reprogram in B16 melanoma into more mature and less aggressive phenotype. Moderated production of ROS/RNS is probably in the background of observed phenomenon. Obtained results are further confirmed in syngeneic mouse model of melanoma in C57BL6 mice. The in vivo results show that SBA-15 do not disturb tumor growth, while both Ph3Sn(CH2)8OH and SBA-15~Cl|Ph3Sn(CH2)8OH significantly decreases tumor volume with an enhancement of the antitumor potential of the tetraorganotin(IV) compound upon immobilization in SBA-15.",
publisher = "Elsevier B.V.",
journal = "Biomaterials Advances",
title = "Effect of chain length on the cytotoxic activity of (alkyl-ω-ol)triphenyltin(IV) loaded into SBA-15 nanostructured silica and in vivo study of SBA-15~Cl|Ph3Sn(CH2)8OH.",
volume = "140",
doi = "10.1016/j.bioadv.2022.213054",
pages = "213054"
}

Bensing, C., Mojić, M., Bulatović, M., Edeler, D., Pérez-Quintanilla, D., Gómez-Ruiz, S., Maksimović-Ivanić, D., Mijatović, S.,& Kaluđerović, G. N.. (2022). Effect of chain length on the cytotoxic activity of (alkyl-ω-ol)triphenyltin(IV) loaded into SBA-15 nanostructured silica and in vivo study of SBA-15~Cl|Ph3Sn(CH2)8OH.. in Biomaterials Advances
Elsevier B.V.., 140, 213054.
https://doi.org/10.1016/j.bioadv.2022.213054

Bensing C, Mojić M, Bulatović M, Edeler D, Pérez-Quintanilla D, Gómez-Ruiz S, Maksimović-Ivanić D, Mijatović S, Kaluđerović GN. Effect of chain length on the cytotoxic activity of (alkyl-ω-ol)triphenyltin(IV) loaded into SBA-15 nanostructured silica and in vivo study of SBA-15~Cl|Ph3Sn(CH2)8OH.. in Biomaterials Advances. 2022;140:213054.
doi:10.1016/j.bioadv.2022.213054 .

Bensing, Christian, Mojić, Marija, Bulatović, Mirna, Edeler, David, Pérez-Quintanilla, Damian, Gómez-Ruiz, Santiago, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Kaluđerović, Goran N., "Effect of chain length on the cytotoxic activity of (alkyl-ω-ol)triphenyltin(IV) loaded into SBA-15 nanostructured silica and in vivo study of SBA-15~Cl|Ph3Sn(CH2)8OH." in Biomaterials Advances, 140 (2022):213054,
https://doi.org/10.1016/j.bioadv.2022.213054 . .

TY  - JOUR
AU  - Drača, Dijana
AU  - Edeler, David
AU  - Saoud, Mohamad
AU  - Dojčinović, Biljana
AU  - Dunđerović, Duško
AU  - Đmura, Goran
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Kaluđerović, Goran N.
PY  - 2021
UR  - internal-pdf://Drača et al. - 2021 - Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells i.pdf
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0162013421000301
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33582397
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4154
AB  - CP (cisplatin) and mesoporous silica SBA-15 (Santa Barbara amorphous 15) loaded with CP (→SBA-15|CP) were tested in vitro and in vivo against low metastatic mouse melanoma B16F1 cell line. SBA-15 only, as drug carrier, is found to be not active, while CP and SBA-15|CP revealed high cytotoxicity in lower μM range. The activity of SBA-15|CP was found similar to the activity of CP alone. Both CP and SBA-15|CP induced inhibition of cell proliferation (carboxyfluorescein succinimidyl ester - CFSE assay) along with G2/M arrest (4',6-diamidino-2-phenylindole - DAPI assay). Apoptosis (Annexin V/ propidium iodide - PI assay), through caspase activation (apostat assay) and nitric oxide (NO) production (diacetate(4-amino-5-methylamino-2',7'-difluorofluorescein-diacetat) - DAF FM assay), was identified as main mode of cell death. However, slight elevated autophagy (acridine orange - AO assay) was detected in treated B16F1 cells. CP and SBA-15|CP did not affect production of ROS (reactive oxygen species) in B16F1 cells. Both SBA-15|CP and CP induced in B16F1 G2 arrest and subsequent senescence. SBA-15|CP, but not CP, blocked the growth of melanoma in C57BL/6 mice. Moreover, hepato- and nephrotoxicity in SBA-15|CP treated animals were diminished in comparison to CP confirming multiply improved antitumor potential of immobilized CP. Outstandingly, SBA-15 boosted in vivo activity and diminished side effects of CP.
PB  - Elsevier BV
T2  - Journal of Inorganic Biochemistry
T1  - Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.
VL  - 217
DO  - 10.1016/j.jinorgbio.2021.111383
SP  - 111383
ER  - 

@article{
author = "Drača, Dijana and Edeler, David and Saoud, Mohamad and Dojčinović, Biljana and Dunđerović, Duško and Đmura, Goran and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Kaluđerović, Goran N.",
year = "2021",
abstract = "CP (cisplatin) and mesoporous silica SBA-15 (Santa Barbara amorphous 15) loaded with CP (→SBA-15|CP) were tested in vitro and in vivo against low metastatic mouse melanoma B16F1 cell line. SBA-15 only, as drug carrier, is found to be not active, while CP and SBA-15|CP revealed high cytotoxicity in lower μM range. The activity of SBA-15|CP was found similar to the activity of CP alone. Both CP and SBA-15|CP induced inhibition of cell proliferation (carboxyfluorescein succinimidyl ester - CFSE assay) along with G2/M arrest (4',6-diamidino-2-phenylindole - DAPI assay). Apoptosis (Annexin V/ propidium iodide - PI assay), through caspase activation (apostat assay) and nitric oxide (NO) production (diacetate(4-amino-5-methylamino-2',7'-difluorofluorescein-diacetat) - DAF FM assay), was identified as main mode of cell death. However, slight elevated autophagy (acridine orange - AO assay) was detected in treated B16F1 cells. CP and SBA-15|CP did not affect production of ROS (reactive oxygen species) in B16F1 cells. Both SBA-15|CP and CP induced in B16F1 G2 arrest and subsequent senescence. SBA-15|CP, but not CP, blocked the growth of melanoma in C57BL/6 mice. Moreover, hepato- and nephrotoxicity in SBA-15|CP treated animals were diminished in comparison to CP confirming multiply improved antitumor potential of immobilized CP. Outstandingly, SBA-15 boosted in vivo activity and diminished side effects of CP.",
publisher = "Elsevier BV",
journal = "Journal of Inorganic Biochemistry",
title = "Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.",
volume = "217",
doi = "10.1016/j.jinorgbio.2021.111383",
pages = "111383"
}

Drača, D., Edeler, D., Saoud, M., Dojčinović, B., Dunđerović, D., Đmura, G., Maksimović-Ivanić, D., Mijatović, S.,& Kaluđerović, G. N.. (2021). Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.. in Journal of Inorganic Biochemistry
Elsevier BV., 217, 111383.
https://doi.org/10.1016/j.jinorgbio.2021.111383

Drača D, Edeler D, Saoud M, Dojčinović B, Dunđerović D, Đmura G, Maksimović-Ivanić D, Mijatović S, Kaluđerović GN. Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.. in Journal of Inorganic Biochemistry. 2021;217:111383.
doi:10.1016/j.jinorgbio.2021.111383 .

Drača, Dijana, Edeler, David, Saoud, Mohamad, Dojčinović, Biljana, Dunđerović, Duško, Đmura, Goran, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Kaluđerović, Goran N., "Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies." in Journal of Inorganic Biochemistry, 217 (2021):111383,
https://doi.org/10.1016/j.jinorgbio.2021.111383 . .

TY  - JOUR
AU  - Maksimović-Ivanić, Danijela
AU  - Bulatović, Mirna
AU  - Edeler, David
AU  - Bensing, Christian
AU  - Golić, Igor
AU  - Korać, Aleksandra
AU  - Kaluđerović, Goran N.
AU  - Mijatović, Sanja
PY  - 2019
UR  - http://link.springer.com/10.1007/s00775-019-01640-x
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3276
AB  - Extraordinary progress in medicinal inorganic chemistry in the past few years led to the rational design of novel platinum compounds, as well as nonplatinum metal-based antitumor agents, including organotin compounds, whose activity is not based on unrepairable interaction with DNA. To overcome poor solubility and toxicity problems that limited the application of these compounds numerous delivering systems were used (Lila et al. in Biol Pharm Bull 37:206–211, 2014; Yue and Cao in Curr Cancer Drug Targets 16:480–488, 2016; Duan et al. in WIREs Nanomed Nanobiotechnol 8:776–791, 2016). Regarding high drug loading capacity, mesoporous silica nanoparticles like SBA-15 became more important for targeted drug delivery. In this study, cellular uptake and biological activities responsible for organotin(IV) compound Ph3Sn(CH2)6OH (Sn6) grafted into (3-chloropropyl)triethoxysilane functionalized SBA-15 (SBA-15p → SBA-15p|Sn6) were evaluated in human melanoma A375 cell line. Moreover, the influence of SBA-15p grafted with organotin(IV) compound on the stemness of A375 cell was tested. Given the fact that SBA-15p|Sn6 nanoparticles are nonspherical and relatively large, their internalization efficiently started even after 15 min with stable adhesion to the cell membrane. After only 2 h of incubation of A375 cells with SBA-15p|Sn6 passive fluid-phase uptake and macropinocytosis were observed. Inside of the cell, treatment with SBA-15p loaded with Sn6 promoted caspase-dependent apoptosis in parallel with senescence development. The subpopulation of cells expressing Schwann-like phenotype arose upon the treatment, while the signaling pathway responsible for maintenance of pluripotency and invasiveness, Wnt, Notch1, and Oct3/4 were modulated towards less aggressive signature. In summary, SBA-15p enhances the efficacy of free Sn6 compound through efficient uptake and well profiled intracellular response followed with decreased stem characteristics of highly invasive A375 melanoma cells.
T2  - JBIC Journal of Biological Inorganic Chemistry
T1  - The interaction between SBA-15 derivative loaded with Ph3Sn(CH2)6OH and human melanoma A375 cell line: uptake and stem phenotype loss
DO  - 10.1007/s00775-019-01640-x
ER  - 

@article{
author = "Maksimović-Ivanić, Danijela and Bulatović, Mirna and Edeler, David and Bensing, Christian and Golić, Igor and Korać, Aleksandra and Kaluđerović, Goran N. and Mijatović, Sanja",
year = "2019",
abstract = "Extraordinary progress in medicinal inorganic chemistry in the past few years led to the rational design of novel platinum compounds, as well as nonplatinum metal-based antitumor agents, including organotin compounds, whose activity is not based on unrepairable interaction with DNA. To overcome poor solubility and toxicity problems that limited the application of these compounds numerous delivering systems were used (Lila et al. in Biol Pharm Bull 37:206–211, 2014; Yue and Cao in Curr Cancer Drug Targets 16:480–488, 2016; Duan et al. in WIREs Nanomed Nanobiotechnol 8:776–791, 2016). Regarding high drug loading capacity, mesoporous silica nanoparticles like SBA-15 became more important for targeted drug delivery. In this study, cellular uptake and biological activities responsible for organotin(IV) compound Ph3Sn(CH2)6OH (Sn6) grafted into (3-chloropropyl)triethoxysilane functionalized SBA-15 (SBA-15p → SBA-15p|Sn6) were evaluated in human melanoma A375 cell line. Moreover, the influence of SBA-15p grafted with organotin(IV) compound on the stemness of A375 cell was tested. Given the fact that SBA-15p|Sn6 nanoparticles are nonspherical and relatively large, their internalization efficiently started even after 15 min with stable adhesion to the cell membrane. After only 2 h of incubation of A375 cells with SBA-15p|Sn6 passive fluid-phase uptake and macropinocytosis were observed. Inside of the cell, treatment with SBA-15p loaded with Sn6 promoted caspase-dependent apoptosis in parallel with senescence development. The subpopulation of cells expressing Schwann-like phenotype arose upon the treatment, while the signaling pathway responsible for maintenance of pluripotency and invasiveness, Wnt, Notch1, and Oct3/4 were modulated towards less aggressive signature. In summary, SBA-15p enhances the efficacy of free Sn6 compound through efficient uptake and well profiled intracellular response followed with decreased stem characteristics of highly invasive A375 melanoma cells.",
journal = "JBIC Journal of Biological Inorganic Chemistry",
title = "The interaction between SBA-15 derivative loaded with Ph3Sn(CH2)6OH and human melanoma A375 cell line: uptake and stem phenotype loss",
doi = "10.1007/s00775-019-01640-x"
}

Maksimović-Ivanić, D., Bulatović, M., Edeler, D., Bensing, C., Golić, I., Korać, A., Kaluđerović, G. N.,& Mijatović, S.. (2019). The interaction between SBA-15 derivative loaded with Ph3Sn(CH2)6OH and human melanoma A375 cell line: uptake and stem phenotype loss. in JBIC Journal of Biological Inorganic Chemistry.
https://doi.org/10.1007/s00775-019-01640-x

Maksimović-Ivanić D, Bulatović M, Edeler D, Bensing C, Golić I, Korać A, Kaluđerović GN, Mijatović S. The interaction between SBA-15 derivative loaded with Ph3Sn(CH2)6OH and human melanoma A375 cell line: uptake and stem phenotype loss. in JBIC Journal of Biological Inorganic Chemistry. 2019;.
doi:10.1007/s00775-019-01640-x .

Maksimović-Ivanić, Danijela, Bulatović, Mirna, Edeler, David, Bensing, Christian, Golić, Igor, Korać, Aleksandra, Kaluđerović, Goran N., Mijatović, Sanja, "The interaction between SBA-15 derivative loaded with Ph3Sn(CH2)6OH and human melanoma A375 cell line: uptake and stem phenotype loss" in JBIC Journal of Biological Inorganic Chemistry (2019),
https://doi.org/10.1007/s00775-019-01640-x . .

TY  - JOUR
AU  - Edeler, David
AU  - Drača, Dijana
AU  - Petković, Vladana
AU  - Natalio, Filipe
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Schmidt, Harry
AU  - Kaluđerović, Goran N.
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0928493118329175?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3302
AB  - Herein appropriateness of nonfunctionalized mesoporous silica nanoparticles SBA-15 and functionalized with (3-chloropropyl)triethoxysilane (→ SBA-15~Cl) and (3-aminopropyl)triethoxysilane (→ SBA-15~NH2) on delivery of physically adsorbed Ph3Sn(CH2)6OH (Sn6) is evaluated. Fluorescent nanomaterial, bearing isatoic moiety, loaded with Sn6 (→ SBA-15~NF|Sn6) was used for cellular uptake study. The fluorescent nanomaterial is efficiently acquired and distributed into the cytoplasm of the cells even after 2 h of cultivation. According to the attained data, all SBA-15 materials loaded with Sn6 diminished cellular viability in dose dependent manner while carriers alone (SBA-15, SBA-15~Cl, SBA-15~NH2) did not show cytotoxicity against B16 cells. According to the MC50 values structural modification of SBA-15 did not improve the efficacy of tested drug. While progressive apoptosis was detected upon the treatment with SBA-15|Sn6, exposure of cells to SBA-15~NH2|Sn6 revealed extinguished apoptosis in time, accompanied with lower caspase activity. This effect is probably due to triggered autophagic process under the treatment with the SBA-15~NH2|Sn6, thus opposed to apoptosis. Presented results suggested that functionalization of SBA-15 was not beneficial for the efficacy of loaded drug, thus, all of them are almost equally efficient considering loaded Sn6 content. Importantly, functionalization of SBA-15 does have an influence on the mode of action and differentiation inducing properties.
T2  - Materials Science and Engineering: C
T1  - Impact of the mesoporous silica SBA-15 functionalization on the mode of action of Ph3Sn(CH2)6OH
VL  - 100
DO  - 10.1016/J.MSEC.2019.03.010
SP  - 315
EP  - 322
ER  - 

@article{
author = "Edeler, David and Drača, Dijana and Petković, Vladana and Natalio, Filipe and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Schmidt, Harry and Kaluđerović, Goran N.",
year = "2019",
abstract = "Herein appropriateness of nonfunctionalized mesoporous silica nanoparticles SBA-15 and functionalized with (3-chloropropyl)triethoxysilane (→ SBA-15~Cl) and (3-aminopropyl)triethoxysilane (→ SBA-15~NH2) on delivery of physically adsorbed Ph3Sn(CH2)6OH (Sn6) is evaluated. Fluorescent nanomaterial, bearing isatoic moiety, loaded with Sn6 (→ SBA-15~NF|Sn6) was used for cellular uptake study. The fluorescent nanomaterial is efficiently acquired and distributed into the cytoplasm of the cells even after 2 h of cultivation. According to the attained data, all SBA-15 materials loaded with Sn6 diminished cellular viability in dose dependent manner while carriers alone (SBA-15, SBA-15~Cl, SBA-15~NH2) did not show cytotoxicity against B16 cells. According to the MC50 values structural modification of SBA-15 did not improve the efficacy of tested drug. While progressive apoptosis was detected upon the treatment with SBA-15|Sn6, exposure of cells to SBA-15~NH2|Sn6 revealed extinguished apoptosis in time, accompanied with lower caspase activity. This effect is probably due to triggered autophagic process under the treatment with the SBA-15~NH2|Sn6, thus opposed to apoptosis. Presented results suggested that functionalization of SBA-15 was not beneficial for the efficacy of loaded drug, thus, all of them are almost equally efficient considering loaded Sn6 content. Importantly, functionalization of SBA-15 does have an influence on the mode of action and differentiation inducing properties.",
journal = "Materials Science and Engineering: C",
title = "Impact of the mesoporous silica SBA-15 functionalization on the mode of action of Ph3Sn(CH2)6OH",
volume = "100",
doi = "10.1016/J.MSEC.2019.03.010",
pages = "315-322"
}

Edeler, D., Drača, D., Petković, V., Natalio, F., Maksimović-Ivanić, D., Mijatović, S., Schmidt, H.,& Kaluđerović, G. N.. (2019). Impact of the mesoporous silica SBA-15 functionalization on the mode of action of Ph3Sn(CH2)6OH. in Materials Science and Engineering: C, 100, 315-322.
https://doi.org/10.1016/J.MSEC.2019.03.010

Edeler D, Drača D, Petković V, Natalio F, Maksimović-Ivanić D, Mijatović S, Schmidt H, Kaluđerović GN. Impact of the mesoporous silica SBA-15 functionalization on the mode of action of Ph3Sn(CH2)6OH. in Materials Science and Engineering: C. 2019;100:315-322.
doi:10.1016/J.MSEC.2019.03.010 .

Edeler, David, Drača, Dijana, Petković, Vladana, Natalio, Filipe, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Schmidt, Harry, Kaluđerović, Goran N., "Impact of the mesoporous silica SBA-15 functionalization on the mode of action of Ph3Sn(CH2)6OH" in Materials Science and Engineering: C, 100 (2019):315-322,
https://doi.org/10.1016/J.MSEC.2019.03.010 . .

TY  - JOUR
AU  - Edeler, David
AU  - Arlt, Sören
AU  - Petković, Vladana
AU  - Ludwig, Gerd
AU  - Drača, Dijana
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Kaluđerović, Goran N.
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0162013417306165
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29288894
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2954
AB  - SBA-15 (Santa Barbara Amorphous 15) mesoporous silica and its functionalized form (with 3-mercaptopropyltriethoxysilane) SBA-15~SH were used as carriers for [Ru(η6-p-cymene)Cl2{Ph2P(CH2)3SPh-κP}] complex, denoted as [Ru]. Prepared mesoporous silica nanomaterials were characterized by traditional methods. Materials without [Ru] complex did not show any cytotoxic activity against melanoma B16 and B16-F10 cell lines. On the contrary, materials containing [Ru] such as SBA-15|[Ru] and SBA-15~SH|[Ru], exhibited very high activity against tested tumor cell lines, moreover with similar inhibitory potential. According to the loaded amount of the [Ru] in SBA-15|[Ru] and SBA-15~SH|[Ru] the IC50 values are 1-2μM depending on the test used, thus in comparison to [Ru] alone the activity of nanomaterials containing [Ru] are elevated 3-6 times in vitro. However, the mechanism of apoptosis induction differs for these two mesoporous silica. Unlike reference [Ru] compound and SBA-15~SH|[Ru], SBA-15|[Ru] induces high caspase activation. Discrepancy in mechanism of drugs action at intracellular level points towards an influence of functionalization as well as availability of the drug. Moreover, both SBA-15|[Ru] and SBA-15~SH|[Ru] similarly to [Ru] are declining autophagy in B16 cell line.
T2  - Journal of Inorganic Biochemistry
T1  - Delivery of [Ru(η6-p-cymene)Cl2{Ph2P(CH2)3SPh-κP}] using unfunctionalized and mercapto functionalized SBA-15 mesoporous silica: Preparation, characterization and in vitro study.
VL  - 180
DO  - 10.1016/j.jinorgbio.2017.12.011
SP  - 155
EP  - 162
ER  - 

@article{
author = "Edeler, David and Arlt, Sören and Petković, Vladana and Ludwig, Gerd and Drača, Dijana and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Kaluđerović, Goran N.",
year = "2018",
abstract = "SBA-15 (Santa Barbara Amorphous 15) mesoporous silica and its functionalized form (with 3-mercaptopropyltriethoxysilane) SBA-15~SH were used as carriers for [Ru(η6-p-cymene)Cl2{Ph2P(CH2)3SPh-κP}] complex, denoted as [Ru]. Prepared mesoporous silica nanomaterials were characterized by traditional methods. Materials without [Ru] complex did not show any cytotoxic activity against melanoma B16 and B16-F10 cell lines. On the contrary, materials containing [Ru] such as SBA-15|[Ru] and SBA-15~SH|[Ru], exhibited very high activity against tested tumor cell lines, moreover with similar inhibitory potential. According to the loaded amount of the [Ru] in SBA-15|[Ru] and SBA-15~SH|[Ru] the IC50 values are 1-2μM depending on the test used, thus in comparison to [Ru] alone the activity of nanomaterials containing [Ru] are elevated 3-6 times in vitro. However, the mechanism of apoptosis induction differs for these two mesoporous silica. Unlike reference [Ru] compound and SBA-15~SH|[Ru], SBA-15|[Ru] induces high caspase activation. Discrepancy in mechanism of drugs action at intracellular level points towards an influence of functionalization as well as availability of the drug. Moreover, both SBA-15|[Ru] and SBA-15~SH|[Ru] similarly to [Ru] are declining autophagy in B16 cell line.",
journal = "Journal of Inorganic Biochemistry",
title = "Delivery of [Ru(η6-p-cymene)Cl2{Ph2P(CH2)3SPh-κP}] using unfunctionalized and mercapto functionalized SBA-15 mesoporous silica: Preparation, characterization and in vitro study.",
volume = "180",
doi = "10.1016/j.jinorgbio.2017.12.011",
pages = "155-162"
}

Edeler, D., Arlt, S., Petković, V., Ludwig, G., Drača, D., Maksimović-Ivanić, D., Mijatović, S.,& Kaluđerović, G. N.. (2018). Delivery of [Ru(η6-p-cymene)Cl2{Ph2P(CH2)3SPh-κP}] using unfunctionalized and mercapto functionalized SBA-15 mesoporous silica: Preparation, characterization and in vitro study.. in Journal of Inorganic Biochemistry, 180, 155-162.
https://doi.org/10.1016/j.jinorgbio.2017.12.011

Edeler D, Arlt S, Petković V, Ludwig G, Drača D, Maksimović-Ivanić D, Mijatović S, Kaluđerović GN. Delivery of [Ru(η6-p-cymene)Cl2{Ph2P(CH2)3SPh-κP}] using unfunctionalized and mercapto functionalized SBA-15 mesoporous silica: Preparation, characterization and in vitro study.. in Journal of Inorganic Biochemistry. 2018;180:155-162.
doi:10.1016/j.jinorgbio.2017.12.011 .

Edeler, David, Arlt, Sören, Petković, Vladana, Ludwig, Gerd, Drača, Dijana, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Kaluđerović, Goran N., "Delivery of [Ru(η6-p-cymene)Cl2{Ph2P(CH2)3SPh-κP}] using unfunctionalized and mercapto functionalized SBA-15 mesoporous silica: Preparation, characterization and in vitro study." in Journal of Inorganic Biochemistry, 180 (2018):155-162,
https://doi.org/10.1016/j.jinorgbio.2017.12.011 . .

TY  - JOUR
AU  - Krajnović, Tamara
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Drača, Dijana
AU  - Wolf, Katharina
AU  - Edeler, David
AU  - Wessjohann, Ludger A
AU  - Kaluđerović, Goran N
PY  - 2018
UR  - http://www.mdpi.com/2079-4991/8/5/322
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5977336
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3078
AB  - In this study mesoporous silica SBA-15 was evaluated as a vehicle for the transport of cytotoxic natural product emodin (EO). SBA-15 was loaded with different quantities of EO (SBA-15|EO1⁻SBA-15|EO5: 8⁻36%) and characterized by traditional methods. Several parameters (stabilities) and the in vitro behavior on tumor cell lines (melanoma A375, B16 and B16F10) were investigated. SBA-15 suppresses EO release in extremely acidic milieu, pointing out that EO will not be discharged in the stomach. Furthermore, SBA-15 protects EO from photodecomposition. In vitro studies showed a dose dependent decrease of cellular viability which is directly correlated with an increasing amount of EO in SBA-15 for up to 27% of EO, while a constant activity for 32% and 36% of EO in SBA-15 was observed. Additionally, SBA-15 loaded with EO (SBA-15|EO3) does not disturb viability of peritoneal macrophages. SBA-15|EO3 causes inhibition of tumor cell proliferation and triggers apoptosis, connected with caspase activation, upregulation of Bax, as well as Bcl-2 and Bim downregulation along with amplification of poly-(ADP-ribose)-polymerase (PARP) cleavage fragment. Thus, the mesoporous SBA-15 is a promising carrier of the water-insoluble drug emodin.
T2  - Nanomaterials (Basel, Switzerland)
T1  - Drug Delivery System for Emodin Based on Mesoporous Silica SBA-15.
IS  - 5
VL  - 8
DO  - 10.3390/nano8050322
SP  - 322
ER  - 

@article{
author = "Krajnović, Tamara and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Drača, Dijana and Wolf, Katharina and Edeler, David and Wessjohann, Ludger A and Kaluđerović, Goran N",
year = "2018",
abstract = "In this study mesoporous silica SBA-15 was evaluated as a vehicle for the transport of cytotoxic natural product emodin (EO). SBA-15 was loaded with different quantities of EO (SBA-15|EO1⁻SBA-15|EO5: 8⁻36%) and characterized by traditional methods. Several parameters (stabilities) and the in vitro behavior on tumor cell lines (melanoma A375, B16 and B16F10) were investigated. SBA-15 suppresses EO release in extremely acidic milieu, pointing out that EO will not be discharged in the stomach. Furthermore, SBA-15 protects EO from photodecomposition. In vitro studies showed a dose dependent decrease of cellular viability which is directly correlated with an increasing amount of EO in SBA-15 for up to 27% of EO, while a constant activity for 32% and 36% of EO in SBA-15 was observed. Additionally, SBA-15 loaded with EO (SBA-15|EO3) does not disturb viability of peritoneal macrophages. SBA-15|EO3 causes inhibition of tumor cell proliferation and triggers apoptosis, connected with caspase activation, upregulation of Bax, as well as Bcl-2 and Bim downregulation along with amplification of poly-(ADP-ribose)-polymerase (PARP) cleavage fragment. Thus, the mesoporous SBA-15 is a promising carrier of the water-insoluble drug emodin.",
journal = "Nanomaterials (Basel, Switzerland)",
title = "Drug Delivery System for Emodin Based on Mesoporous Silica SBA-15.",
number = "5",
volume = "8",
doi = "10.3390/nano8050322",
pages = "322"
}

Krajnović, T., Maksimović-Ivanić, D., Mijatović, S., Drača, D., Wolf, K., Edeler, D., Wessjohann, L. A.,& Kaluđerović, G. N.. (2018). Drug Delivery System for Emodin Based on Mesoporous Silica SBA-15.. in Nanomaterials (Basel, Switzerland), 8(5), 322.
https://doi.org/10.3390/nano8050322

Krajnović T, Maksimović-Ivanić D, Mijatović S, Drača D, Wolf K, Edeler D, Wessjohann LA, Kaluđerović GN. Drug Delivery System for Emodin Based on Mesoporous Silica SBA-15.. in Nanomaterials (Basel, Switzerland). 2018;8(5):322.
doi:10.3390/nano8050322 .

Krajnović, Tamara, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Drača, Dijana, Wolf, Katharina, Edeler, David, Wessjohann, Ludger A, Kaluđerović, Goran N, "Drug Delivery System for Emodin Based on Mesoporous Silica SBA-15." in Nanomaterials (Basel, Switzerland), 8, no. 5 (2018):322,
https://doi.org/10.3390/nano8050322 . .