TY  - JOUR
AU  - Nikolić, Tatjana
AU  - Kojić, Danijela
AU  - Orčić, Snežana
AU  - Vukašinović, Elvira
AU  - Blagojević, Duško
AU  - Purać, Jelena
PY  - 2019
UR  - http://link.springer.com/10.1007/s11356-018-3950-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3236
AB  - In the present study, the influence of cadmium, copper, and lead on two enzymes often used as biomarkers in toxicological analysis was investigated. Bees were fed with 1 M sucrose solution containing 10-fold serial dilutions of CuCl2 (1000 mg L-1, 100 mg L-1, and 10 mg L-1), CdCl2 (0.1 mg L-1, 0.01 mg L-1, and 0.001 mg L-1), or PbCl2 (10 mg L-1, 1 mg L-1, and 0.1 mg L-1) during 48 h. Our results showed that the total glutathione S-transferase activity was not changed under the influence of cadmium and lead, and it was decreased with the highest concentration of copper. The level of gene expression of the three analyzed classes of glutathione S-transferase was significantly increased with increasing concentrations of copper and cadmium. Lead did not cause significant changes in glutathione S-transferase activity and gene expression, while it showed biphasic effect on acetylcholinesterase activity: lower concentration of lead, 0.1 mg L-1 inhibited and higher dose, 10 mg L-1 induced acetylcholinesterase activity in honey bees. Furthermore, our results showed a significant decrease of the acetylcholinesterase activity in honey bees treated with 0.001 and 0.01 mg L-1 CdCl2. Our results indicate the influence of cadmium, copper, and lead on GST and AChE in the honey bees. These results form the basis for future research on the impact of metallic trace element pollution on honey bees.
T2  - Environmental Science and Pollution Research
T1  - Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.
DO  - 10.1007/s11356-018-3950-6
ER  - 

@article{
author = "Nikolić, Tatjana and Kojić, Danijela and Orčić, Snežana and Vukašinović, Elvira and Blagojević, Duško and Purać, Jelena",
year = "2019",
abstract = "In the present study, the influence of cadmium, copper, and lead on two enzymes often used as biomarkers in toxicological analysis was investigated. Bees were fed with 1 M sucrose solution containing 10-fold serial dilutions of CuCl2 (1000 mg L-1, 100 mg L-1, and 10 mg L-1), CdCl2 (0.1 mg L-1, 0.01 mg L-1, and 0.001 mg L-1), or PbCl2 (10 mg L-1, 1 mg L-1, and 0.1 mg L-1) during 48 h. Our results showed that the total glutathione S-transferase activity was not changed under the influence of cadmium and lead, and it was decreased with the highest concentration of copper. The level of gene expression of the three analyzed classes of glutathione S-transferase was significantly increased with increasing concentrations of copper and cadmium. Lead did not cause significant changes in glutathione S-transferase activity and gene expression, while it showed biphasic effect on acetylcholinesterase activity: lower concentration of lead, 0.1 mg L-1 inhibited and higher dose, 10 mg L-1 induced acetylcholinesterase activity in honey bees. Furthermore, our results showed a significant decrease of the acetylcholinesterase activity in honey bees treated with 0.001 and 0.01 mg L-1 CdCl2. Our results indicate the influence of cadmium, copper, and lead on GST and AChE in the honey bees. These results form the basis for future research on the impact of metallic trace element pollution on honey bees.",
journal = "Environmental Science and Pollution Research",
title = "Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.",
doi = "10.1007/s11356-018-3950-6"
}

Nikolić, T., Kojić, D., Orčić, S., Vukašinović, E., Blagojević, D.,& Purać, J.. (2019). Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.. in Environmental Science and Pollution Research.
https://doi.org/10.1007/s11356-018-3950-6

Nikolić T, Kojić D, Orčić S, Vukašinović E, Blagojević D, Purać J. Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.. in Environmental Science and Pollution Research. 2019;.
doi:10.1007/s11356-018-3950-6 .

Nikolić, Tatjana, Kojić, Danijela, Orčić, Snežana, Vukašinović, Elvira, Blagojević, Duško, Purać, Jelena, "Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead." in Environmental Science and Pollution Research (2019),
https://doi.org/10.1007/s11356-018-3950-6 . .

TY  - JOUR
AU  - Purać, Jelena
AU  - Nikolić, Tatjana V.
AU  - Kojić, Danijela
AU  - Ćelić, Anđelka S.
AU  - Plavša, Jovana J.
AU  - Blagojević, Duško
AU  - Petri, Edward T.
PY  - 2019
UR  - http://doi.wiley.com/10.1111/mec.14984
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3279
AB  - Metallothioneins are ubiquitous proteins important in metal homeostasis and detoxification. However, they have not previously been identified in honey bees or other Hymenoptera, where metallothioneins could be of ecophysiological and ecotoxicological significance. Better understanding of the molecular responses to stress induced by toxic metals could contribute to honey bee conservation. In addition, honey bee metallothionein could represent a biomarker for monitoring environmental quality. Here we identify and characterize a metallothionein gene in Apis mellifera (AmMT). AmMT is 1,680 bp long and encodes a 48 amino acids protein with 15 cysteines and no aromatic residues. A metal response element upstream of the start codon, coupled with numerous cis-regulatory elements indicate the functional context of AmMT. Molecular modelling predicts several transition metal binding sites, and comparative phylogenetic analysis revealed five putative metallothionein proteins in three other hymenoptera species. AmMT was characterized by cloning the full-length coding sequence of the putative metallothionein. Recombinant AmMT was found to increase metal tolerance upon overexpression in Escherichia coli supplemented with Cd, Cu or Pb. Finally, in laboratory tests on honey bees, gene expression profiles showed a dose-dependant relationship between Cd, Cu and Pb concentrations present in food and AmMT expression, while field experiments showed induction of AmMT in bees from an industrial site compared to those from an urban area. These studies suggest that AmMT has metal binding properties in agreement with a possible role in metal homeostasis. Further functional and structural characterization of metallothionein in honey bees and other Hymenoptera are necessary.
T2  - Molecular Ecology
T1  - Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure.
IS  - 4
VL  - 28
DO  - 10.1111/mec.14984
SP  - 731
EP  - 745
ER  - 

@article{
author = "Purać, Jelena and Nikolić, Tatjana V. and Kojić, Danijela and Ćelić, Anđelka S. and Plavša, Jovana J. and Blagojević, Duško and Petri, Edward T.",
year = "2019",
abstract = "Metallothioneins are ubiquitous proteins important in metal homeostasis and detoxification. However, they have not previously been identified in honey bees or other Hymenoptera, where metallothioneins could be of ecophysiological and ecotoxicological significance. Better understanding of the molecular responses to stress induced by toxic metals could contribute to honey bee conservation. In addition, honey bee metallothionein could represent a biomarker for monitoring environmental quality. Here we identify and characterize a metallothionein gene in Apis mellifera (AmMT). AmMT is 1,680 bp long and encodes a 48 amino acids protein with 15 cysteines and no aromatic residues. A metal response element upstream of the start codon, coupled with numerous cis-regulatory elements indicate the functional context of AmMT. Molecular modelling predicts several transition metal binding sites, and comparative phylogenetic analysis revealed five putative metallothionein proteins in three other hymenoptera species. AmMT was characterized by cloning the full-length coding sequence of the putative metallothionein. Recombinant AmMT was found to increase metal tolerance upon overexpression in Escherichia coli supplemented with Cd, Cu or Pb. Finally, in laboratory tests on honey bees, gene expression profiles showed a dose-dependant relationship between Cd, Cu and Pb concentrations present in food and AmMT expression, while field experiments showed induction of AmMT in bees from an industrial site compared to those from an urban area. These studies suggest that AmMT has metal binding properties in agreement with a possible role in metal homeostasis. Further functional and structural characterization of metallothionein in honey bees and other Hymenoptera are necessary.",
journal = "Molecular Ecology",
title = "Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure.",
number = "4",
volume = "28",
doi = "10.1111/mec.14984",
pages = "731-745"
}

Purać, J., Nikolić, T. V., Kojić, D., Ćelić, A. S., Plavša, J. J., Blagojević, D.,& Petri, E. T.. (2019). Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure.. in Molecular Ecology, 28(4), 731-745.
https://doi.org/10.1111/mec.14984

Purać J, Nikolić TV, Kojić D, Ćelić AS, Plavša JJ, Blagojević D, Petri ET. Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure.. in Molecular Ecology. 2019;28(4):731-745.
doi:10.1111/mec.14984 .

Purać, Jelena, Nikolić, Tatjana V., Kojić, Danijela, Ćelić, Anđelka S., Plavša, Jovana J., Blagojević, Duško, Petri, Edward T., "Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure." in Molecular Ecology, 28, no. 4 (2019):731-745,
https://doi.org/10.1111/mec.14984 . .

TY  - JOUR
AU  - Kojić, Danijela K.
AU  - Purać, Jelena S.
AU  - Nikolić, Tatjana V.
AU  - Orčić, Snežana M.
AU  - Vujanović, Dragana
AU  - Ilijević, Konstantin
AU  - Vukašinović, Elvira L.
AU  - Blagojević, Duško
PY  - 2019
UR  - http://www.schweizerbart.de/papers/entomologia/detail/39/91600/Oxidative_stress_and_the_activity_of_antioxidative?af=crossref
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3453
AB  - Over the past decades, the number of managed honey bee Apis mellifera L. (Hymenoptera: Apidae) colonies have been decreasing. The majority of losses occur during winter, suggesting that overwintering honey bees are more susceptible to adverse factors. We focused on the oxidative status of overwintering honey bees, particularly at the beginning (November) and end (March) of the wintering period. Colonies from three locations with different anthropogenic influences were selected: Belgrade, an urban zone, Zajača, an industrial zone, and Susek, a rural area. We measured levels of malondialdehyde (MDA), as a marker of lipid peroxidation, as well as the expression and activity of select antioxidative enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Our results show that enzyme activity and gene expression of antioxidative enzymes are influenced by both sample location and the time of sampling. The majority of analyzed genes had significantly reduced expression, at the end of the overwintering period when higher activities of antioxidative enzymes were also recorded. Among the analyzed parameters, SOD activity and gene expression of microsomal GST isoforms were more affected by local environmental conditions, suggesting the complex role of these enzymes in antioxidative defense and detoxification. The higher MDA levels observed at the end of overwintering for all three locations likely reflects accumulated oxidative damage which could be associated with the aging process, brood rearing and/or the onset flying activity.
T2  - Entomologia Generalis
T1  - Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees
IS  - 1
VL  - 39
DO  - 10.1127/entomologia/2019/0743
SP  - 33
EP  - 44
ER  - 

@article{
author = "Kojić, Danijela K. and Purać, Jelena S. and Nikolić, Tatjana V. and Orčić, Snežana M. and Vujanović, Dragana and Ilijević, Konstantin and Vukašinović, Elvira L. and Blagojević, Duško",
year = "2019",
abstract = "Over the past decades, the number of managed honey bee Apis mellifera L. (Hymenoptera: Apidae) colonies have been decreasing. The majority of losses occur during winter, suggesting that overwintering honey bees are more susceptible to adverse factors. We focused on the oxidative status of overwintering honey bees, particularly at the beginning (November) and end (March) of the wintering period. Colonies from three locations with different anthropogenic influences were selected: Belgrade, an urban zone, Zajača, an industrial zone, and Susek, a rural area. We measured levels of malondialdehyde (MDA), as a marker of lipid peroxidation, as well as the expression and activity of select antioxidative enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Our results show that enzyme activity and gene expression of antioxidative enzymes are influenced by both sample location and the time of sampling. The majority of analyzed genes had significantly reduced expression, at the end of the overwintering period when higher activities of antioxidative enzymes were also recorded. Among the analyzed parameters, SOD activity and gene expression of microsomal GST isoforms were more affected by local environmental conditions, suggesting the complex role of these enzymes in antioxidative defense and detoxification. The higher MDA levels observed at the end of overwintering for all three locations likely reflects accumulated oxidative damage which could be associated with the aging process, brood rearing and/or the onset flying activity.",
journal = "Entomologia Generalis",
title = "Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees",
number = "1",
volume = "39",
doi = "10.1127/entomologia/2019/0743",
pages = "33-44"
}

Kojić, D. K., Purać, J. S., Nikolić, T. V., Orčić, S. M., Vujanović, D., Ilijević, K., Vukašinović, E. L.,& Blagojević, D.. (2019). Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees. in Entomologia Generalis, 39(1), 33-44.
https://doi.org/10.1127/entomologia/2019/0743

Kojić DK, Purać JS, Nikolić TV, Orčić SM, Vujanović D, Ilijević K, Vukašinović EL, Blagojević D. Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees. in Entomologia Generalis. 2019;39(1):33-44.
doi:10.1127/entomologia/2019/0743 .

Kojić, Danijela K., Purać, Jelena S., Nikolić, Tatjana V., Orčić, Snežana M., Vujanović, Dragana, Ilijević, Konstantin, Vukašinović, Elvira L., Blagojević, Duško, "Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees" in Entomologia Generalis, 39, no. 1 (2019):33-44,
https://doi.org/10.1127/entomologia/2019/0743 . .

TY  - JOUR
AU  - Velimirović, Milica
AU  - Jevtić Dožudić, Gordana
AU  - Selaković, Vesna
AU  - Stojković, Tihomir
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - Živković, Milica
AU  - Dragutinović, Vesna
AU  - Nikolić, Tatjana
AU  - Jelenković, Ankica
AU  - Đorović, Đorđe
AU  - Mirčić, Aleksandar
AU  - Petronijević, Nataša D.
PY  - 2018
UR  - https://www.hindawi.com/journals/omcl/2018/3273654/
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5932460
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3167
AB  - Decreased blood flow in the brain leads to a rapid increase in reactive oxygen species (ROS). NADPH oxidase (NOX) is an enzyme family that has the physiological function to produce ROS. NOX2 and NOX4 overexpression is associated with aggravated ischemic injury, while NOX2/4-deficient mice had reduced stroke size. Dysregulation of matrix metalloproteinases (MMPs) contributes to tissue damage. The active form of vitamin D3 expresses neuroprotective, immunomodulatory, and anti-inflammatory effects in the CNS. The present study examines the effects of the vitamin D3 pretreatment on the oxidative stress parameters and the expression of NOX subunits, MMP9, microglial marker Iba1, and vitamin D receptor (VDR), in the cortex and hippocampus of Mongolian gerbils subjected to ten minutes of global cerebral ischemia, followed by 24 hours of reperfusion. The ischemia/reperfusion procedure has induced oxidative stress, changes in the expression of NOX2 subunits and MMP9 in the brain, and increased MMP9 activity in the serum of experimental animals. Pretreatment with vitamin D3 was especially effective on NOX2 subunits, MMP9, and the level of malondialdehyde and superoxide anion. These results outline the significance of the NOX and MMP9 investigation in brain ischemia and the importance of adequate vitamin D supplementation in ameliorating the injury caused by I/R.
T2  - Oxidative medicine and cellular longevity
T1  - Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.
VL  - 2018
DO  - 10.1155/2018/3273654
SP  - 3273654
ER  - 

@article{
author = "Velimirović, Milica and Jevtić Dožudić, Gordana and Selaković, Vesna and Stojković, Tihomir and Puškaš, Nela and Zaletel, Ivan and Živković, Milica and Dragutinović, Vesna and Nikolić, Tatjana and Jelenković, Ankica and Đorović, Đorđe and Mirčić, Aleksandar and Petronijević, Nataša D.",
year = "2018",
abstract = "Decreased blood flow in the brain leads to a rapid increase in reactive oxygen species (ROS). NADPH oxidase (NOX) is an enzyme family that has the physiological function to produce ROS. NOX2 and NOX4 overexpression is associated with aggravated ischemic injury, while NOX2/4-deficient mice had reduced stroke size. Dysregulation of matrix metalloproteinases (MMPs) contributes to tissue damage. The active form of vitamin D3 expresses neuroprotective, immunomodulatory, and anti-inflammatory effects in the CNS. The present study examines the effects of the vitamin D3 pretreatment on the oxidative stress parameters and the expression of NOX subunits, MMP9, microglial marker Iba1, and vitamin D receptor (VDR), in the cortex and hippocampus of Mongolian gerbils subjected to ten minutes of global cerebral ischemia, followed by 24 hours of reperfusion. The ischemia/reperfusion procedure has induced oxidative stress, changes in the expression of NOX2 subunits and MMP9 in the brain, and increased MMP9 activity in the serum of experimental animals. Pretreatment with vitamin D3 was especially effective on NOX2 subunits, MMP9, and the level of malondialdehyde and superoxide anion. These results outline the significance of the NOX and MMP9 investigation in brain ischemia and the importance of adequate vitamin D supplementation in ameliorating the injury caused by I/R.",
journal = "Oxidative medicine and cellular longevity",
title = "Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.",
volume = "2018",
doi = "10.1155/2018/3273654",
pages = "3273654"
}

Velimirović, M., Jevtić Dožudić, G., Selaković, V., Stojković, T., Puškaš, N., Zaletel, I., Živković, M., Dragutinović, V., Nikolić, T., Jelenković, A., Đorović, Đ., Mirčić, A.,& Petronijević, N. D.. (2018). Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.. in Oxidative medicine and cellular longevity, 2018, 3273654.
https://doi.org/10.1155/2018/3273654

Velimirović M, Jevtić Dožudić G, Selaković V, Stojković T, Puškaš N, Zaletel I, Živković M, Dragutinović V, Nikolić T, Jelenković A, Đorović Đ, Mirčić A, Petronijević ND. Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.. in Oxidative medicine and cellular longevity. 2018;2018:3273654.
doi:10.1155/2018/3273654 .

Velimirović, Milica, Jevtić Dožudić, Gordana, Selaković, Vesna, Stojković, Tihomir, Puškaš, Nela, Zaletel, Ivan, Živković, Milica, Dragutinović, Vesna, Nikolić, Tatjana, Jelenković, Ankica, Đorović, Đorđe, Mirčić, Aleksandar, Petronijević, Nataša D., "Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia." in Oxidative medicine and cellular longevity, 2018 (2018):3273654,
https://doi.org/10.1155/2018/3273654 . .

TY  - JOUR
AU  - Orčić, Snežana
AU  - Nikolić, Tatjana
AU  - Purać, Jelena
AU  - Šikoparija, Branko
AU  - Blagojević, Duško
AU  - Vukašinović, Elvira
AU  - Plavša, Nada
AU  - Stevanović, Jevrosima
AU  - Kojić, Danijela
PY  - 2017
UR  - http://doi.wiley.com/10.1111/eea.12633
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2964
AB  - The recent decline in managed honey bee populations, Apis mellifera L. (Hymenoptera: Apidae), has caused scientific, ecological, and economic concern. Research into the formation of reactive oxygen species (ROS), antioxidative defense mechanisms, and oxidative stress can contribute to our understanding of bee survival and conservation of this species. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) enzymes together with levels of malondialdehyde (MDA) were measured in summer and winter honey bees sampled from three colonies. One colony was stationary (C1), entering the winter period having accumulated Robinia pseudoacacia L. (Fabaceae) honey, and two were migratory (C2 and C3), entering the winter period with mainly Tilia (Malvaceae) and Brassica (Brassicaceae) honey, respectively. Compared to summer workers, winter worker bees had decreased SOD and GST activity, and MDA level, whereas CAT activity increased in all three colonies. We also demonstrated that seasonality is the main factor responsible for changes in antioxidant enzymes and MDA levels in worker honey bees. Overall, our results indicate a difference between summer and winter worker bees, pointing at a reduced level of antioxidant enzyme defenses during overwintering which may be due to a decrease in production of ROS. The decreased levels of MDA measured in winter honey bees confirm this. As ROS are actively used by insects as a defense mechanism to fight pathogens, we suggest that reduced production of ROS contributes to higher susceptibility of winter honey bees to infections and reduced overwinter survival.
T2  - Entomologia Experimentalis et Applicata
T1  - Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees
IS  - 2-3
VL  - 165
DO  - 10.1111/eea.12633
SP  - 120
EP  - 128
ER  - 

@article{
author = "Orčić, Snežana and Nikolić, Tatjana and Purać, Jelena and Šikoparija, Branko and Blagojević, Duško and Vukašinović, Elvira and Plavša, Nada and Stevanović, Jevrosima and Kojić, Danijela",
year = "2017",
abstract = "The recent decline in managed honey bee populations, Apis mellifera L. (Hymenoptera: Apidae), has caused scientific, ecological, and economic concern. Research into the formation of reactive oxygen species (ROS), antioxidative defense mechanisms, and oxidative stress can contribute to our understanding of bee survival and conservation of this species. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) enzymes together with levels of malondialdehyde (MDA) were measured in summer and winter honey bees sampled from three colonies. One colony was stationary (C1), entering the winter period having accumulated Robinia pseudoacacia L. (Fabaceae) honey, and two were migratory (C2 and C3), entering the winter period with mainly Tilia (Malvaceae) and Brassica (Brassicaceae) honey, respectively. Compared to summer workers, winter worker bees had decreased SOD and GST activity, and MDA level, whereas CAT activity increased in all three colonies. We also demonstrated that seasonality is the main factor responsible for changes in antioxidant enzymes and MDA levels in worker honey bees. Overall, our results indicate a difference between summer and winter worker bees, pointing at a reduced level of antioxidant enzyme defenses during overwintering which may be due to a decrease in production of ROS. The decreased levels of MDA measured in winter honey bees confirm this. As ROS are actively used by insects as a defense mechanism to fight pathogens, we suggest that reduced production of ROS contributes to higher susceptibility of winter honey bees to infections and reduced overwinter survival.",
journal = "Entomologia Experimentalis et Applicata",
title = "Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees",
number = "2-3",
volume = "165",
doi = "10.1111/eea.12633",
pages = "120-128"
}

Orčić, S., Nikolić, T., Purać, J., Šikoparija, B., Blagojević, D., Vukašinović, E., Plavša, N., Stevanović, J.,& Kojić, D.. (2017). Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees. in Entomologia Experimentalis et Applicata, 165(2-3), 120-128.
https://doi.org/10.1111/eea.12633

Orčić S, Nikolić T, Purać J, Šikoparija B, Blagojević D, Vukašinović E, Plavša N, Stevanović J, Kojić D. Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees. in Entomologia Experimentalis et Applicata. 2017;165(2-3):120-128.
doi:10.1111/eea.12633 .

Orčić, Snežana, Nikolić, Tatjana, Purać, Jelena, Šikoparija, Branko, Blagojević, Duško, Vukašinović, Elvira, Plavša, Nada, Stevanović, Jevrosima, Kojić, Danijela, "Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees" in Entomologia Experimentalis et Applicata, 165, no. 2-3 (2017):120-128,
https://doi.org/10.1111/eea.12633 . .

TY  - JOUR
AU  - Nikolić, Tatjana V.
AU  - Kojić, Danijela
AU  - Orčić, Snežana
AU  - Batinić, Darko
AU  - Vukašinović, Elvira
AU  - Blagojević, Duško
AU  - Purać, Jelena
PY  - 2016
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27580263
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0045653516311006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2611
AB  - In this study, laboratory bioassays were performed to investigate the impact of sublethal concentrations of Cu (CuCl2: 1000, 100, 10 mg L(-1)), Pb (PbCl2: 10, 1, 0.1 mg L(-1)) and Cd (CdCl2: 0.1, 0.01, 0.001 mg L(-1)) on honey bee redox status and the activity of the main antioxidative enzymes and their gene expression. Our results show that exposure to these metals led to significant changes of gene expression, the levels of enzyme activity and redox status, but the effects are metal and dose dependent. In general, exposure of 48 h to given concentrations of Cu, Cd and Pb did not change the activity of antioxidative enzymes and the level of lipid peroxidation, with the exception of decreased activity of catalase at the lowest concentration of cadmium. Only lead produced increases in glutathione and thiol groups. Expression of genes for catalase and superoxide dismutase changed with exposure to cadmium and copper, whilst lead induced only expression of superoxide dismutase genes. The results from this study provide basic data for future research regarding the impacts of metal pollution on Apis mellifera and will be an important step towards a comprehensive risk assessment of the environmental stressors on honey bees.
T2  - Chemosphere
T1  - The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions.
VL  - 164
DO  - 10.1016/j.chemosphere.2016.08.077
SP  - 98
EP  - 105
ER  - 

@article{
author = "Nikolić, Tatjana V. and Kojić, Danijela and Orčić, Snežana and Batinić, Darko and Vukašinović, Elvira and Blagojević, Duško and Purać, Jelena",
year = "2016",
abstract = "In this study, laboratory bioassays were performed to investigate the impact of sublethal concentrations of Cu (CuCl2: 1000, 100, 10 mg L(-1)), Pb (PbCl2: 10, 1, 0.1 mg L(-1)) and Cd (CdCl2: 0.1, 0.01, 0.001 mg L(-1)) on honey bee redox status and the activity of the main antioxidative enzymes and their gene expression. Our results show that exposure to these metals led to significant changes of gene expression, the levels of enzyme activity and redox status, but the effects are metal and dose dependent. In general, exposure of 48 h to given concentrations of Cu, Cd and Pb did not change the activity of antioxidative enzymes and the level of lipid peroxidation, with the exception of decreased activity of catalase at the lowest concentration of cadmium. Only lead produced increases in glutathione and thiol groups. Expression of genes for catalase and superoxide dismutase changed with exposure to cadmium and copper, whilst lead induced only expression of superoxide dismutase genes. The results from this study provide basic data for future research regarding the impacts of metal pollution on Apis mellifera and will be an important step towards a comprehensive risk assessment of the environmental stressors on honey bees.",
journal = "Chemosphere",
title = "The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions.",
volume = "164",
doi = "10.1016/j.chemosphere.2016.08.077",
pages = "98-105"
}

Nikolić, T. V., Kojić, D., Orčić, S., Batinić, D., Vukašinović, E., Blagojević, D.,& Purać, J.. (2016). The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions.. in Chemosphere, 164, 98-105.
https://doi.org/10.1016/j.chemosphere.2016.08.077

Nikolić TV, Kojić D, Orčić S, Batinić D, Vukašinović E, Blagojević D, Purać J. The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions.. in Chemosphere. 2016;164:98-105.
doi:10.1016/j.chemosphere.2016.08.077 .

Nikolić, Tatjana V., Kojić, Danijela, Orčić, Snežana, Batinić, Darko, Vukašinović, Elvira, Blagojević, Duško, Purać, Jelena, "The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions." in Chemosphere, 164 (2016):98-105,
https://doi.org/10.1016/j.chemosphere.2016.08.077 . .

TY  - JOUR
AU  - Popović, Željko
AU  - Subotić, Ana
AU  - Nikolić, Tatjana
AU  - Radojičić, Ratko
AU  - Blagojević, Duško
AU  - Grubor-Lajšić, Gordana
AU  - Koštál, Vladimír
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6336
AB  - Diapause is a state of arrested development during which insects cope with many external and internal stressful factors. European corn borer, Ostrinia nubilalis, overwinters as a fifth instar freeze-tolerant diapausing larva. In order to explore diapause-linked stress tolerance processes, the expression of selected genes coding for stress related proteins—glutathione S-transferase (Gst), thioredoxin (Trx), glutaredoxin (Grx), ferritin (Fer), metallothionein (Mtn), and heat shock proteins Hsp90, Hsc70, Hsp20.4, and Hsp20.1—was assessed in the fat body of diapause-destined, warm (22 °C) and cold (5 °C) acclimated diapausing larvae using the quantitative real-time PCR. Gene expression was normalised to mRNA transcripts for Actin and Rps03, and relative expression was calculated using non-diapausing larvae as a control group. During the initiation phase of diapause, the abundance of mRNA transcripts of Grx, Hsp90, Hsc70, andHsp20.1 was significantly upregulated, Trx, Fer, Mtn, andHsp20.1 were unchanged, while only Gst was clearly downregulated in comparison to non-diapause control. Later, in the early phase of diapause, the expression of most genes (except Trx and Hsp20.1) was upregulated in warm-acclimated
larvae, while only Trx and Hsp90 were upregulated in cold-acclimated larvae. Furthermore, the relative expression of all genes (except Trx) increased gradually throughout the diapause in cold-acclimated larvae. This result indicates that the half-life of mRNAs is prolonged during diapause at low temperature, which may lead to a gradual accumulation of mRNA transcripts. Our results show that both diapause programming and temperatures affect the expression of stress-related genes in Ostrinia nubilalis.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology, Part B
T1  - Expression of stress-related genes in diapause of European corn borer (Ostrinia nubilalis Hbn.)
VL  - 186
DO  - 10.1016/j.cbpb.2015.04.004
SP  - 1
EP  - 7
ER  - 

@article{
author = "Popović, Željko and Subotić, Ana and Nikolić, Tatjana and Radojičić, Ratko and Blagojević, Duško and Grubor-Lajšić, Gordana and Koštál, Vladimír",
year = "2015",
abstract = "Diapause is a state of arrested development during which insects cope with many external and internal stressful factors. European corn borer, Ostrinia nubilalis, overwinters as a fifth instar freeze-tolerant diapausing larva. In order to explore diapause-linked stress tolerance processes, the expression of selected genes coding for stress related proteins—glutathione S-transferase (Gst), thioredoxin (Trx), glutaredoxin (Grx), ferritin (Fer), metallothionein (Mtn), and heat shock proteins Hsp90, Hsc70, Hsp20.4, and Hsp20.1—was assessed in the fat body of diapause-destined, warm (22 °C) and cold (5 °C) acclimated diapausing larvae using the quantitative real-time PCR. Gene expression was normalised to mRNA transcripts for Actin and Rps03, and relative expression was calculated using non-diapausing larvae as a control group. During the initiation phase of diapause, the abundance of mRNA transcripts of Grx, Hsp90, Hsc70, andHsp20.1 was significantly upregulated, Trx, Fer, Mtn, andHsp20.1 were unchanged, while only Gst was clearly downregulated in comparison to non-diapause control. Later, in the early phase of diapause, the expression of most genes (except Trx and Hsp20.1) was upregulated in warm-acclimated
larvae, while only Trx and Hsp90 were upregulated in cold-acclimated larvae. Furthermore, the relative expression of all genes (except Trx) increased gradually throughout the diapause in cold-acclimated larvae. This result indicates that the half-life of mRNAs is prolonged during diapause at low temperature, which may lead to a gradual accumulation of mRNA transcripts. Our results show that both diapause programming and temperatures affect the expression of stress-related genes in Ostrinia nubilalis.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology, Part B",
title = "Expression of stress-related genes in diapause of European corn borer (Ostrinia nubilalis Hbn.)",
volume = "186",
doi = "10.1016/j.cbpb.2015.04.004",
pages = "1-7"
}

Popović, Ž., Subotić, A., Nikolić, T., Radojičić, R., Blagojević, D., Grubor-Lajšić, G.,& Koštál, V.. (2015). Expression of stress-related genes in diapause of European corn borer (Ostrinia nubilalis Hbn.). in Comparative Biochemistry and Physiology, Part B
Elsevier Inc.., 186, 1-7.
https://doi.org/10.1016/j.cbpb.2015.04.004

Popović Ž, Subotić A, Nikolić T, Radojičić R, Blagojević D, Grubor-Lajšić G, Koštál V. Expression of stress-related genes in diapause of European corn borer (Ostrinia nubilalis Hbn.). in Comparative Biochemistry and Physiology, Part B. 2015;186:1-7.
doi:10.1016/j.cbpb.2015.04.004 .

Popović, Željko, Subotić, Ana, Nikolić, Tatjana, Radojičić, Ratko, Blagojević, Duško, Grubor-Lajšić, Gordana, Koštál, Vladimír, "Expression of stress-related genes in diapause of European corn borer (Ostrinia nubilalis Hbn.)" in Comparative Biochemistry and Physiology, Part B, 186 (2015):1-7,
https://doi.org/10.1016/j.cbpb.2015.04.004 . .