@conference{
author = "Anđus, Stefan and Vasiljević, Božica and Lazović, Vladimir and Paunović, Momir",
year = "2019",
abstract = "Freshwater sponges belong to macrozoobenthic organisms with a worldwide distribution.
European rivers and lakes, most generally, have been well analyzed for the presence of Porifera
[1]-[5]. Some geographical areas, however, remain largely unexplored. Among these are West
Balkan countries. The aim of the present study was to determine sponge species collected in
Macedonia and Serbia based on the specific morphology of their mineral skeleton, using light
and scanning electron microscopy (SEM). In total 122 specimens were collected and spicules
were prepared by nitric acid technique [6]. Determination was mainly performed by observing
sponge structures under light microscope. For fine analysis of sponge skeleton elements, in
some cases, SEM was applied allowing measurements of these elements to be done for an
accurate species determination (Figure 1.).
As revealed by the length and width of sponge spicules, as well as their shape, among the
collected specimens the following species have been identified: in Serbia- Spongilla lacustris
Linnaeus, 1758, Eunapius fragilis (Leidy, 1851), Ephydatia fluviatilis (Linnaeus, 1759),
Ephydatia muelleri (Lieberkühn, 1856), and Trochospongilla horrida Weltner, 1893; in
Macedonia S. lacustris, E. fluviatilis, S stankovici Arndt 1937, Spongilla carteri Bowerbank,
1863 and Ochridaspongia rotunda (Arndt 1937). In conclusion, SEM made possible an
efficient assessment of fine sponge skeletal structure needed for species identification.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia",
title = "Scanning electron microscopy-a sensitive tool in Porifera determination",
pages = "166-167",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5601"
}
