TY  - JOUR
AU  - Prijović, Mladen
AU  - Nikolić, Bogdan
AU  - Dragićević, Ivana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Giba, Zlatko
AU  - Jovanović, Vladan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6562
AB  - Plant protection with natural products is a new trend in environmentally friendly agriculture.
Nepeta rtanjensis Diklić et Milojević is an endemic and critically endangered plant species in Serbia.
We explored the phytotoxic potential of a water emulsion of Nepeta rtanjensis essential oil (NrEO)
with high amounts of trans,cis-nepetalactone on five weeds. The most sensitive was Stellaria media
(L.) Vill., as NrEO (from 0.013% to 0.1%) completely inhibited germination. Germination of
Amaranthus retroflexus L. and Artemisia vulgaris L. was completely inhibited at the highest applied
concentrations of NrEO (0.1%), while Ambrosia artemisiifolia L. germination was reduced to 48% at
the same concentration of NrEO. The most tolerant species was Cephalaria transsylvanica (L.) Schrad.
ex Roem. & Schult. as the final germination rate at the highest applied concentration of NrEO (0.1%)
was 81%, like the control (82%). To our knowledge, this is the first time the interaction of essential oils
on the germination and growth of A. vulgaris and C. transsylvanica is reported. The germination
dynamics of S. media in pots with soil were significantly inhibited when the soil was initially treated
with the highest applied concentration of the NrEO water emulsion (1%). Spraying S. media seedlings
with NrEO significantly inhibited growth parameters (shoot height, shoot fresh weight, and the number
of nodes) at the higher applied concentrations of NrEO (0.5% and 1%). Therefore, the water emulsion
of the essential oil of N. rtanjensis could be potentially developed for use in the control of invasive and
allergenic weeds.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide
DO  - 10.2298/ABS231107041P
ER  - 

@article{
author = "Prijović, Mladen and Nikolić, Bogdan and Dragićević, Ivana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Giba, Zlatko and Jovanović, Vladan",
year = "2023",
abstract = "Plant protection with natural products is a new trend in environmentally friendly agriculture.
Nepeta rtanjensis Diklić et Milojević is an endemic and critically endangered plant species in Serbia.
We explored the phytotoxic potential of a water emulsion of Nepeta rtanjensis essential oil (NrEO)
with high amounts of trans,cis-nepetalactone on five weeds. The most sensitive was Stellaria media
(L.) Vill., as NrEO (from 0.013% to 0.1%) completely inhibited germination. Germination of
Amaranthus retroflexus L. and Artemisia vulgaris L. was completely inhibited at the highest applied
concentrations of NrEO (0.1%), while Ambrosia artemisiifolia L. germination was reduced to 48% at
the same concentration of NrEO. The most tolerant species was Cephalaria transsylvanica (L.) Schrad.
ex Roem. & Schult. as the final germination rate at the highest applied concentration of NrEO (0.1%)
was 81%, like the control (82%). To our knowledge, this is the first time the interaction of essential oils
on the germination and growth of A. vulgaris and C. transsylvanica is reported. The germination
dynamics of S. media in pots with soil were significantly inhibited when the soil was initially treated
with the highest applied concentration of the NrEO water emulsion (1%). Spraying S. media seedlings
with NrEO significantly inhibited growth parameters (shoot height, shoot fresh weight, and the number
of nodes) at the higher applied concentrations of NrEO (0.5% and 1%). Therefore, the water emulsion
of the essential oil of N. rtanjensis could be potentially developed for use in the control of invasive and
allergenic weeds.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide",
doi = "10.2298/ABS231107041P"
}

Prijović, M., Nikolić, B., Dragićević, I., Nestorović Živković, J., Dmitrović, S., Giba, Z.,& Jovanović, V.. (2023). Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide. in Archives of Biological Sciences
Belgrade: Serbian Biological Society..
https://doi.org/10.2298/ABS231107041P

Prijović M, Nikolić B, Dragićević I, Nestorović Živković J, Dmitrović S, Giba Z, Jovanović V. Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide. in Archives of Biological Sciences. 2023;.
doi:10.2298/ABS231107041P .

Prijović, Mladen, Nikolić, Bogdan, Dragićević, Ivana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Giba, Zlatko, Jovanović, Vladan, "Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide" in Archives of Biological Sciences (2023),
https://doi.org/10.2298/ABS231107041P . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Stamenković, Nikola
AU  - Đurić, Marija
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Giba, Zlatko
AU  - Subotić, Angelina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5335
AB  - Soil salinity is one of the most common abiotic stressors that affects plant growth and
development. The aim of this work was to investigate the influence of sodium nitroprusside (SNP),
a donor of nitric oxide (NO), on the physiological response of common centaury (Centaurium erythraea)
shoots grown under stress conditions caused by sodium chloride (NaCl) in vitro. Centaury
shoots were first grown on nutrient medium containing different SNP concentrations (50, 100 and
250 μM) during the pretreatment phase. After three weeks, the shoots were transferred to nutrient
media supplemented with NaCl (150 mM) and/or SNP (50, 100 or 250 μM) for one week. The results
showed that salinity decreased photosynthetic pigments, total phenolic content and DPPH (1,1-diphenyl-
2-picrylhydrazyl radical) concentration. The activities of antioxidant enzymes, namely superoxide
dismutase (SOD), catalase (CAT) and peroxidase (POX), were also reduced under salt
stress. However, MDA concentration was decreased, while H2O2 and proline content did not drastically
change under the stress conditions caused by NaCl. Exogenous application of SNP altered
the biochemical parameters of centaury shoots grown under salt stress. In this case, increased photosynthetic
pigment content, total phenolics and proline content were noted, with reduced MDA,
but not H2O2, concentration was observed. In addition, the exogenous application of SNP increased
the degree of DPPH reduction as well as SOD, CAT and POX activities.
PB  - Basel: MDPI
T2  - Life
T1  - Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro
IS  - 1
VL  - 13
DO  - 10.3390/life13010154
SP  - 154
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Stamenković, Nikola and Đurić, Marija and Milošević, Snežana and Marković, Marija and Giba, Zlatko and Subotić, Angelina",
year = "2023",
abstract = "Soil salinity is one of the most common abiotic stressors that affects plant growth and
development. The aim of this work was to investigate the influence of sodium nitroprusside (SNP),
a donor of nitric oxide (NO), on the physiological response of common centaury (Centaurium erythraea)
shoots grown under stress conditions caused by sodium chloride (NaCl) in vitro. Centaury
shoots were first grown on nutrient medium containing different SNP concentrations (50, 100 and
250 μM) during the pretreatment phase. After three weeks, the shoots were transferred to nutrient
media supplemented with NaCl (150 mM) and/or SNP (50, 100 or 250 μM) for one week. The results
showed that salinity decreased photosynthetic pigments, total phenolic content and DPPH (1,1-diphenyl-
2-picrylhydrazyl radical) concentration. The activities of antioxidant enzymes, namely superoxide
dismutase (SOD), catalase (CAT) and peroxidase (POX), were also reduced under salt
stress. However, MDA concentration was decreased, while H2O2 and proline content did not drastically
change under the stress conditions caused by NaCl. Exogenous application of SNP altered
the biochemical parameters of centaury shoots grown under salt stress. In this case, increased photosynthetic
pigment content, total phenolics and proline content were noted, with reduced MDA,
but not H2O2, concentration was observed. In addition, the exogenous application of SNP increased
the degree of DPPH reduction as well as SOD, CAT and POX activities.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro",
number = "1",
volume = "13",
doi = "10.3390/life13010154",
pages = "154"
}

Trifunović-Momčilov, M., Stamenković, N., Đurić, M., Milošević, S., Marković, M., Giba, Z.,& Subotić, A.. (2023). Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro. in Life
Basel: MDPI., 13(1), 154.
https://doi.org/10.3390/life13010154

Trifunović-Momčilov M, Stamenković N, Đurić M, Milošević S, Marković M, Giba Z, Subotić A. Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro. in Life. 2023;13(1):154.
doi:10.3390/life13010154 .

Trifunović-Momčilov, Milana, Stamenković, Nikola, Đurić, Marija, Milošević, Snežana, Marković, Marija, Giba, Zlatko, Subotić, Angelina, "Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro" in Life, 13, no. 1 (2023):154,
https://doi.org/10.3390/life13010154 . .

TY  - CONF
AU  - Simonović, Milica
AU  - Nestorović Živković, Jasmina
AU  - Bjedov, Ivana
AU  - Nešić, Marija
AU  - Jovanović, Vladan
AU  - Giba, Zlatko
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Dmitrović, Slavica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5569
AB  - The use of bioherbicides offers the possibility to suppress harmful weeds by ecologically acceptable methods and thus increase crop productivity. Progress in understanding the mechanisms of bioherbicides action and weed defense response mechanisms, could help in defining
novel weed control strategies. This work highlights the bioherbicide potential of rhizomes of Sorghum halepense L., characterized by a high content of phenolic acids, especially p-hydroxybenzoic
acid (pHB A) and chlorogenic acid (CHL A). Methanol extract of S. halepense rhizome (SHR), and its
major bioactive principles, displayed phytotoxic effects against Chenopodium murale L., which is a
common weed of fields and roadsides. Besides reduction in C. murale seed germination, rhizome
extract of S. halepense applied in the concentrations of 0.01, 0.1, 1 and 2 mg/ml, induced changes
in the activity of antioxidant enzymes in seedlings. Catalase (CAT) activity was increased, while
peroxidase (POX) and superoxide dismutase (SOD) activities were generally decreased, with a few
exceptions (increased POX and SOD activities at 0.1 mg/ml at 0.01 mg/ml SHR, respectively), suggesting that CAT is the major responsible for the elimination of reactive oxygen species generated
during the exposure of C. murale seedlings to SHR. Application of CHL A induced changes in the
activities of antioxidant enzymes, similar to those observed on SHR treatments. The treatment
with pHB A did not significantly change the activity of POX and SOD, while CAT activity was significantly increased at all applied concentrations. These data highlight the phytotoxic potential as
a highly efficient strategy of S. halepense to invade new habitats.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5569
ER  - 

@conference{
author = "Simonović, Milica and Nestorović Živković, Jasmina and Bjedov, Ivana and Nešić, Marija and Jovanović, Vladan and Giba, Zlatko and Gašić, Uroš and Mišić, Danijela and Dmitrović, Slavica",
year = "2022",
abstract = "The use of bioherbicides offers the possibility to suppress harmful weeds by ecologically acceptable methods and thus increase crop productivity. Progress in understanding the mechanisms of bioherbicides action and weed defense response mechanisms, could help in defining
novel weed control strategies. This work highlights the bioherbicide potential of rhizomes of Sorghum halepense L., characterized by a high content of phenolic acids, especially p-hydroxybenzoic
acid (pHB A) and chlorogenic acid (CHL A). Methanol extract of S. halepense rhizome (SHR), and its
major bioactive principles, displayed phytotoxic effects against Chenopodium murale L., which is a
common weed of fields and roadsides. Besides reduction in C. murale seed germination, rhizome
extract of S. halepense applied in the concentrations of 0.01, 0.1, 1 and 2 mg/ml, induced changes
in the activity of antioxidant enzymes in seedlings. Catalase (CAT) activity was increased, while
peroxidase (POX) and superoxide dismutase (SOD) activities were generally decreased, with a few
exceptions (increased POX and SOD activities at 0.1 mg/ml at 0.01 mg/ml SHR, respectively), suggesting that CAT is the major responsible for the elimination of reactive oxygen species generated
during the exposure of C. murale seedlings to SHR. Application of CHL A induced changes in the
activities of antioxidant enzymes, similar to those observed on SHR treatments. The treatment
with pHB A did not significantly change the activity of POX and SOD, while CAT activity was significantly increased at all applied concentrations. These data highlight the phytotoxic potential as
a highly efficient strategy of S. halepense to invade new habitats.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5569"
}

Simonović, M., Nestorović Živković, J., Bjedov, I., Nešić, M., Jovanović, V., Giba, Z., Gašić, U., Mišić, D.,& Dmitrović, S.. (2022). Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 80.
https://hdl.handle.net/21.15107/rcub_ibiss_5569

Simonović M, Nestorović Živković J, Bjedov I, Nešić M, Jovanović V, Giba Z, Gašić U, Mišić D, Dmitrović S. Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:80.
https://hdl.handle.net/21.15107/rcub_ibiss_5569 .

Simonović, Milica, Nestorović Živković, Jasmina, Bjedov, Ivana, Nešić, Marija, Jovanović, Vladan, Giba, Zlatko, Gašić, Uroš, Mišić, Danijela, Dmitrović, Slavica, "Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):80,
https://hdl.handle.net/21.15107/rcub_ibiss_5569 .

TY  - CONF
AU  - Đurić, Marija
AU  - Stamenković, Nikola
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Giba, Zlatko
AU  - Subotić, Angelina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5327
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant used in traditional medicine
since ancient times. Centaury inhabits dry pastures, roadsides and fields from lowlands to
mountain vegetation. Considering that centaury can also be found on moderately saline habitats
in nature, it was interesting to investigate the effect of exogenously applied sodium nitroprusside
(SNP), as a donor of nitrogen monoxide (NO), on antioxidative enzymes activity of centaury shoots
grown under stress caused by sodium chloride (NaCl) in vitro. Centaury shoots were grown on
½MS nutrient media containing graded concentrations of SNP (0, 50, 100 and 250 μM). After three
weeks of pretreatment, shoots were further transferred to ½MS nutrient media supplemented
with NaCl (0 or 150 mM) and/or SNP (0, 50, 100 or 250 μM) during one week treatment. After four
weeks, the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT)
and peroxidase (POX) was determined spectrophotometrically. Thus, through 14 different treatments,
the effect of NaCl and SNP on biochemical response of centaury shoots was investigated.
The results obtained in this work showed that NaCl decreased the activity of SOD, CAT and POX.
On the other hand, exogenous application of SNP increased SOD, CAT and POX activity in centaury
shoots after four weeks. It can be concluded that the effect of SNP on increased tolerance of
centaury shoots to salinity is reflected through the changed activity of enzymatic components of
antioxidant defence.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5327
ER  - 

@conference{
author = "Đurić, Marija and Stamenković, Nikola and Trifunović-Momčilov, Milana and Milošević, Snežana and Marković, Marija and Giba, Zlatko and Subotić, Angelina",
year = "2022",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant used in traditional medicine
since ancient times. Centaury inhabits dry pastures, roadsides and fields from lowlands to
mountain vegetation. Considering that centaury can also be found on moderately saline habitats
in nature, it was interesting to investigate the effect of exogenously applied sodium nitroprusside
(SNP), as a donor of nitrogen monoxide (NO), on antioxidative enzymes activity of centaury shoots
grown under stress caused by sodium chloride (NaCl) in vitro. Centaury shoots were grown on
½MS nutrient media containing graded concentrations of SNP (0, 50, 100 and 250 μM). After three
weeks of pretreatment, shoots were further transferred to ½MS nutrient media supplemented
with NaCl (0 or 150 mM) and/or SNP (0, 50, 100 or 250 μM) during one week treatment. After four
weeks, the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT)
and peroxidase (POX) was determined spectrophotometrically. Thus, through 14 different treatments,
the effect of NaCl and SNP on biochemical response of centaury shoots was investigated.
The results obtained in this work showed that NaCl decreased the activity of SOD, CAT and POX.
On the other hand, exogenous application of SNP increased SOD, CAT and POX activity in centaury
shoots after four weeks. It can be concluded that the effect of SNP on increased tolerance of
centaury shoots to salinity is reflected through the changed activity of enzymatic components of
antioxidant defence.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro",
pages = "60",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5327"
}

Đurić, M., Stamenković, N., Trifunović-Momčilov, M., Milošević, S., Marković, M., Giba, Z.,& Subotić, A.. (2022). The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 60.
https://hdl.handle.net/21.15107/rcub_ibiss_5327

Đurić M, Stamenković N, Trifunović-Momčilov M, Milošević S, Marković M, Giba Z, Subotić A. The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:60.
https://hdl.handle.net/21.15107/rcub_ibiss_5327 .

Đurić, Marija, Stamenković, Nikola, Trifunović-Momčilov, Milana, Milošević, Snežana, Marković, Marija, Giba, Zlatko, Subotić, Angelina, "The effect of sodium nitroprusside on antioxidative enzymes activity of centaury (Centaurium erythraea Rafn) under stress conditions caused by sodium chloride in vitro" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):60,
https://hdl.handle.net/21.15107/rcub_ibiss_5327 .

TY  - CONF
AU  - Trifunović-Momčilov, Milana
AU  - Stamenković, Nikola
AU  - Đurić, Marija
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Giba, Zlatko
AU  - Subotić, Angelina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5377
AB  - Кичица (Centaurium erythraea Rafn) је лековита биљна која се у народној медицини 
од давнина употребљава као лек за снижавање температуре, регулацију нивоа 
шећера у крви, а такође и за лечење анемије, жутице и гихта. У природи кичица је 
врло честа на сувим пашњацима, ободу путева и њива. Познато је да заслањене 
подлоге могу проузроковати прогресиван губитак хлорофила у листовима биљака. 
Такође је познато да су биљке које живе на заслањеним стаништима развиле 
механизме који им омогућавају да одрже фотосинтезу у присуству повишене 
концентрације соли. Имајући у виду да кичица у природи насељава и благо 
заслањена земљишта, испитиван је утицај егзогено примењеног натријум 
нитропрусида (SNP), као донора азот моноксида, на садржај фотосинтетичких 
пигмената изданака кичице гајених in vitro у условима стреса изазваног натријум 
хлоридом (NaCl). У овом раду приказан је позитиван утицај SNP на концентрацију 
укупних хлорофила у условима стреса изазваног NaCl. Најбоље резултате, у 
смислу повишеног нивоа укупног хлорофила у односу на контролне изданке гајене 
у присуству NaCl, показала је најнижа примењена концентрације SNP. Са друге 
стране резултати у овом раду показују да је SNP утицао на повећање биосинтезе 
каротеноида у условима стреса изазваног NaCl. Уочено је да је повећање 
концентрације укупних каротеноида у корелацији са примењеним концентрацијама 
SNP. На основу добијених резултата може се закључити да примена SNP има 
смисла када је кичица изложена стресу изазваном NaCl.
AB  - Kičica (Centaurium erythraea Rafn) je lekovita biljna koja se u narodnoj medicini od davnina upotrebljava kao lek za snižavanje temperature, regulaciju nivoa šećera u krvi, a takođe i za lečenje anemije, žutice i gihta. U prirodi kičica je vrlo česta na suvim pašnjacima, obodu puteva i njiva. Poznato je da zaslanjene podloge mogu prouzrokovati progresivan gubitak hlorofila u listovima biljaka. Takođe je poznato da su biljke koje žive na zaslanjenim staništima razvile mehanizme koji im omogućavaju da održe fotosintezu u prisustvu povišene koncentracije soli. Imajući u vidu da kičica u prirodi naseljava i blago zaslanjena zemljišta, ispitivan je uticaj egzogeno primenjenog natrijum nitroprusida (SNP), kao donora azot monoksida, na sadržaj fotosintetičkih pigmenata izdanaka kičice gajenih in vitro u uslovima stresa izazvanog natrijum hloridom (NaCl). U ovom radu prikazan je pozitivan uticaj SNP na koncentraciju ukupnih hlorofila u uslovima stresa izazvanog NaCl. Najbolje rezultate, u smislu povišenog nivoa ukupnog hlorofila u odnosu na kontrolne izdanke gajene u prisustvu NaCl, pokazala je najniža primenjena koncentracije SNP. Sa druge strane rezultati u ovom radu pokazuju da je SNP uticao na povećanje biosinteze karotenoida u uslovima stresa izazvanog NaCl. Uočeno je da je povećanje koncentracije ukupnih karotenoida u korelaciji sa primenjenim koncentracijama SNP. Na osnovu dobijenih rezultata može se zaključiti da primena SNP ima smisla kada je kičica izložena stresu izazvanom NaCl.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом
T1  - Uticaj natrijum nitroprusida na sadržaj fotosintetičkih pigmenata kičice (Centaurium erythraea Rafn) gajene in vitro u uslovima stresa izazvanog natrijum hloridom
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5377
ER  - 

@conference{
author = "Trifunović-Momčilov, Milana and Stamenković, Nikola and Đurić, Marija and Milošević, Snežana and Marković, Marija and Giba, Zlatko and Subotić, Angelina",
year = "2022",
abstract = "Кичица (Centaurium erythraea Rafn) је лековита биљна која се у народној медицини 
од давнина употребљава као лек за снижавање температуре, регулацију нивоа 
шећера у крви, а такође и за лечење анемије, жутице и гихта. У природи кичица је 
врло честа на сувим пашњацима, ободу путева и њива. Познато је да заслањене 
подлоге могу проузроковати прогресиван губитак хлорофила у листовима биљака. 
Такође је познато да су биљке које живе на заслањеним стаништима развиле 
механизме који им омогућавају да одрже фотосинтезу у присуству повишене 
концентрације соли. Имајући у виду да кичица у природи насељава и благо 
заслањена земљишта, испитиван је утицај егзогено примењеног натријум 
нитропрусида (SNP), као донора азот моноксида, на садржај фотосинтетичких 
пигмената изданака кичице гајених in vitro у условима стреса изазваног натријум 
хлоридом (NaCl). У овом раду приказан је позитиван утицај SNP на концентрацију 
укупних хлорофила у условима стреса изазваног NaCl. Најбоље резултате, у 
смислу повишеног нивоа укупног хлорофила у односу на контролне изданке гајене 
у присуству NaCl, показала је најнижа примењена концентрације SNP. Са друге 
стране резултати у овом раду показују да је SNP утицао на повећање биосинтезе 
каротеноида у условима стреса изазваног NaCl. Уочено је да је повећање 
концентрације укупних каротеноида у корелацији са примењеним концентрацијама 
SNP. На основу добијених резултата може се закључити да примена SNP има 
смисла када је кичица изложена стресу изазваном NaCl., Kičica (Centaurium erythraea Rafn) je lekovita biljna koja se u narodnoj medicini od davnina upotrebljava kao lek za snižavanje temperature, regulaciju nivoa šećera u krvi, a takođe i za lečenje anemije, žutice i gihta. U prirodi kičica je vrlo česta na suvim pašnjacima, obodu puteva i njiva. Poznato je da zaslanjene podloge mogu prouzrokovati progresivan gubitak hlorofila u listovima biljaka. Takođe je poznato da su biljke koje žive na zaslanjenim staništima razvile mehanizme koji im omogućavaju da održe fotosintezu u prisustvu povišene koncentracije soli. Imajući u vidu da kičica u prirodi naseljava i blago zaslanjena zemljišta, ispitivan je uticaj egzogeno primenjenog natrijum nitroprusida (SNP), kao donora azot monoksida, na sadržaj fotosintetičkih pigmenata izdanaka kičice gajenih in vitro u uslovima stresa izazvanog natrijum hloridom (NaCl). U ovom radu prikazan je pozitivan uticaj SNP na koncentraciju ukupnih hlorofila u uslovima stresa izazvanog NaCl. Najbolje rezultate, u smislu povišenog nivoa ukupnog hlorofila u odnosu na kontrolne izdanke gajene u prisustvu NaCl, pokazala je najniža primenjena koncentracije SNP. Sa druge strane rezultati u ovom radu pokazuju da je SNP uticao na povećanje biosinteze karotenoida u uslovima stresa izazvanog NaCl. Uočeno je da je povećanje koncentracije ukupnih karotenoida u korelaciji sa primenjenim koncentracijama SNP. Na osnovu dobijenih rezultata može se zaključiti da primena SNP ima smisla kada je kičica izložena stresu izazvanom NaCl.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом, Uticaj natrijum nitroprusida na sadržaj fotosintetičkih pigmenata kičice (Centaurium erythraea Rafn) gajene in vitro u uslovima stresa izazvanog natrijum hloridom",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5377"
}

Trifunović-Momčilov, M., Stamenković, N., Đurić, M., Milošević, S., Marković, M., Giba, Z.,& Subotić, A.. (2022). Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 69.
https://hdl.handle.net/21.15107/rcub_ibiss_5377

Trifunović-Momčilov M, Stamenković N, Đurić M, Milošević S, Marković M, Giba Z, Subotić A. Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:69.
https://hdl.handle.net/21.15107/rcub_ibiss_5377 .

Trifunović-Momčilov, Milana, Stamenković, Nikola, Đurić, Marija, Milošević, Snežana, Marković, Marija, Giba, Zlatko, Subotić, Angelina, "Утицај натријум нитропрусида на садржај фотосинтетичких  пигмената кичице (Centaurium erythraea Rafn) гајене in vitro у  условима стреса изазваног натријум хлоридом" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):69,
https://hdl.handle.net/21.15107/rcub_ibiss_5377 .

TY  - CONF
AU  - Todorović, Slađana
AU  - Banjanac, Tijana
AU  - Bogdanović, Milica
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
PY  - 2011
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6176
AB  - Many chicory varieties (Cichorium intybus L., Asteraceae) are important agricultural and medicinal plants used throughout Europe. The bitter taste of chicory is associated with the presence of large quantities of sesquiterpene lactones. Some of the guanolides isolated from chicory, play role as antifeedants and phytoalexins, and possess cytotoxic activity towards cultured cancer cells, while the root extracts have anti-inflammatory and hepatoprotective activities. Hairy roots obtained by A. rhizogenes transformation grow fast and do not require hormones in the medium. The greatest advantage of hairy roots is that they often exhibit greater biosynthetic capacity for secondary metabolite
production compared to the mother plants. The main scope of our investigation was to optimize protocols for transformation of two chicory cultivars, C. intybus blue and C. intybus catalogna, with A. rhizogenes bearing 35S-GUS reporter vector and to establish optimal conditions for hairy roots growth. Transformation was confirmed in 7 C. intybus blue and in 5 C. intybus catalogna clones by PCR with GUS-specific primers. The GUS expression was confirmed in 6 C. intybus blue and 4 C. intybus catalogna clones by RT-PCR and histochemical GUS staining. Two of the transformed clones of C. intybus catalogna explained the greatest biomass production and growth potential. The established transformation protocol will be the basis for future planned transformation experiments and for the large-scale production of target secundary metabolites using hairy root cultures.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia
T1  - In vitro culture establishment and biomass production of Cichorium intybus L. hairy roots
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6176
ER  - 

@conference{
author = "Todorović, Slađana and Banjanac, Tijana and Bogdanović, Milica and Dragićević, Milan and Simonović, Ana and Giba, Zlatko and Grubišić, Dragoljub",
year = "2011",
abstract = "Many chicory varieties (Cichorium intybus L., Asteraceae) are important agricultural and medicinal plants used throughout Europe. The bitter taste of chicory is associated with the presence of large quantities of sesquiterpene lactones. Some of the guanolides isolated from chicory, play role as antifeedants and phytoalexins, and possess cytotoxic activity towards cultured cancer cells, while the root extracts have anti-inflammatory and hepatoprotective activities. Hairy roots obtained by A. rhizogenes transformation grow fast and do not require hormones in the medium. The greatest advantage of hairy roots is that they often exhibit greater biosynthetic capacity for secondary metabolite
production compared to the mother plants. The main scope of our investigation was to optimize protocols for transformation of two chicory cultivars, C. intybus blue and C. intybus catalogna, with A. rhizogenes bearing 35S-GUS reporter vector and to establish optimal conditions for hairy roots growth. Transformation was confirmed in 7 C. intybus blue and in 5 C. intybus catalogna clones by PCR with GUS-specific primers. The GUS expression was confirmed in 6 C. intybus blue and 4 C. intybus catalogna clones by RT-PCR and histochemical GUS staining. Two of the transformed clones of C. intybus catalogna explained the greatest biomass production and growth potential. The established transformation protocol will be the basis for future planned transformation experiments and for the large-scale production of target secundary metabolites using hairy root cultures.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia",
title = "In vitro culture establishment and biomass production of Cichorium intybus L. hairy roots",
pages = "79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6176"
}

Todorović, S., Banjanac, T., Bogdanović, M., Dragićević, M., Simonović, A., Giba, Z.,& Grubišić, D.. (2011). In vitro culture establishment and biomass production of Cichorium intybus L. hairy roots. in Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia
Belgrade: Serbian Plant Physiology Society., 79.
https://hdl.handle.net/21.15107/rcub_ibiss_6176

Todorović S, Banjanac T, Bogdanović M, Dragićević M, Simonović A, Giba Z, Grubišić D. In vitro culture establishment and biomass production of Cichorium intybus L. hairy roots. in Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia. 2011;:79.
https://hdl.handle.net/21.15107/rcub_ibiss_6176 .

Todorović, Slađana, Banjanac, Tijana, Bogdanović, Milica, Dragićević, Milan, Simonović, Ana, Giba, Zlatko, Grubišić, Dragoljub, "In vitro culture establishment and biomass production of Cichorium intybus L. hairy roots" in Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia (2011):79,
https://hdl.handle.net/21.15107/rcub_ibiss_6176 .