TY  - CONF
AU  - Ćosić, Tatjana
AU  - Devrnja, Nina
AU  - Krstić Milošević, Dijana
AU  - Božić, Dragana
AU  - Jančić, Ivan
AU  - Raspor, Martin
AU  - Maksimović, Zoran
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5365
AB  - In Serbia, citral chemotype of Thymus pannonicus All. (Lamiaceae) can be only found on Mt
Vršačke planine, as dominant form, where is traditionally used for medicinal purposes. To overcome
difficulties in harvesting due to distribution in inaccessible areas, and to prevent possible
overexploitation from its natural habitat, finding the means for alternative cultivation of this plant
for its prospective beneficial use in human health care was set as objective of this study. Efficient
system for in vitro shoot propagation of T. pannonicus was established for the first time. Different
types of auxin (indole-3-acetic acid, IAA; indole-3-butyric acid, IBA; 1-naphthaleneacetic acid, NAA)
were used for testing the process of rhizogenesis in vitro. Best response was detected on IBA and
NAA for rhizogenesis frequency and number of formed roots, respectively. Rooted plants were
successfully acclimatized. In vitro shoots cultivated on PGR free medium (E1) and medium with 1
mg L-1 IAA (E2) were extracted with 80% methanol and analysed by HPLC. Presence of rosmarinic
acid (RA) as dominant component was indicated in both extracts, with E2 containing higher RA
amount (45.116 mg g-1DW). Antimicrobial activity of extracts was tested using broth microdilution
method against nine strains of laboratory control pathogenic microorganisms. Both extracts
exerted significant antimicrobial activity against all tested pathogens with minimal inhibitory concen-
trations (MIC) in the range of 6.25-50 mg mL-1, while E2 was more effective against Gram positive
bacteria (6.25-12.5 mg mL-1). Presented results could give inducement for potential greater
commercial use of this significant species in the future.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Potential of Thymus pannonicus All. in vitro culture: micropropagation, rosmarinic acid production and antimicrobial activity
SP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5365
ER  - 

@conference{
author = "Ćosić, Tatjana and Devrnja, Nina and Krstić Milošević, Dijana and Božić, Dragana and Jančić, Ivan and Raspor, Martin and Maksimović, Zoran",
year = "2022",
abstract = "In Serbia, citral chemotype of Thymus pannonicus All. (Lamiaceae) can be only found on Mt
Vršačke planine, as dominant form, where is traditionally used for medicinal purposes. To overcome
difficulties in harvesting due to distribution in inaccessible areas, and to prevent possible
overexploitation from its natural habitat, finding the means for alternative cultivation of this plant
for its prospective beneficial use in human health care was set as objective of this study. Efficient
system for in vitro shoot propagation of T. pannonicus was established for the first time. Different
types of auxin (indole-3-acetic acid, IAA; indole-3-butyric acid, IBA; 1-naphthaleneacetic acid, NAA)
were used for testing the process of rhizogenesis in vitro. Best response was detected on IBA and
NAA for rhizogenesis frequency and number of formed roots, respectively. Rooted plants were
successfully acclimatized. In vitro shoots cultivated on PGR free medium (E1) and medium with 1
mg L-1 IAA (E2) were extracted with 80% methanol and analysed by HPLC. Presence of rosmarinic
acid (RA) as dominant component was indicated in both extracts, with E2 containing higher RA
amount (45.116 mg g-1DW). Antimicrobial activity of extracts was tested using broth microdilution
method against nine strains of laboratory control pathogenic microorganisms. Both extracts
exerted significant antimicrobial activity against all tested pathogens with minimal inhibitory concen-
trations (MIC) in the range of 6.25-50 mg mL-1, while E2 was more effective against Gram positive
bacteria (6.25-12.5 mg mL-1). Presented results could give inducement for potential greater
commercial use of this significant species in the future.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Potential of Thymus pannonicus All. in vitro culture: micropropagation, rosmarinic acid production and antimicrobial activity",
pages = "104",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5365"
}

Ćosić, T., Devrnja, N., Krstić Milošević, D., Božić, D., Jančić, I., Raspor, M.,& Maksimović, Z.. (2022). Potential of Thymus pannonicus All. in vitro culture: micropropagation, rosmarinic acid production and antimicrobial activity. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 104.
https://hdl.handle.net/21.15107/rcub_ibiss_5365

Ćosić T, Devrnja N, Krstić Milošević D, Božić D, Jančić I, Raspor M, Maksimović Z. Potential of Thymus pannonicus All. in vitro culture: micropropagation, rosmarinic acid production and antimicrobial activity. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:104.
https://hdl.handle.net/21.15107/rcub_ibiss_5365 .

Ćosić, Tatjana, Devrnja, Nina, Krstić Milošević, Dijana, Božić, Dragana, Jančić, Ivan, Raspor, Martin, Maksimović, Zoran, "Potential of Thymus pannonicus All. in vitro culture: micropropagation, rosmarinic acid production and antimicrobial activity" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):104,
https://hdl.handle.net/21.15107/rcub_ibiss_5365 .

TY  - JOUR
AU  - Čilović-Kozarević, Ermina
AU  - Šarić-Kundalić, Broza
AU  - Ibišević, Merima
AU  - Horozić, Emir
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Arsenijević, Jelena
AU  - Maksimović, Zoran
PY  - 2021
UR  - https://scindeks.ceon.rs/Article.aspx?artid=0455-62242141035Q
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4933
AB  - Telekia speciosa (Schreb.) Baumg., Asteraceae, is widespread in Eastern and Central Europe and the Balkan Peninsula. Previous phytochemical investigations have revealed T. speciosa as a rich source of sesquiterpene lactone -isoalantolactone, especially in its underground parts. The aim of the present study was to analyze the essential oils from aerial and underground parts of T. speciosa and investigate their antimicrobial activity. Chemical composition of essential oils was determined by GC-FID/MS method leading to the identification of 67 compounds in total, with 15.77 % oxygenated monoterpenes, 7.77 % sesquiterpene hydrocarbons, 49.14 % oxygenated sesquiterpenes, and 12.37 % other compounds from aerial parts, and 3.80 % oxygenated monoterpenes, 3.13 % sesquiterpene hydrocarbons, 90.33 % oxygenated sesquiterpenes from underground parts essential oil. The main components from aerial parts were (E)-nerolidol (11.54 %) and caryophyllene oxide (10.54 %), while isoalantolactone was the predominant component from essential oil underground parts (83.41 %). The minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration of the essential oils were evaluated against six strains of bacteria and two strains of fungus using in vitro microdilution method. Both oils presented antimicrobial properties against pathogens Staphylococcus aureus, Bacilus cereus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. Inhibition of growth of tested microorganisms by T. speciosa underground parts essential oil was achieved with MICs ranging from 1.0 to 11.0 mg mL -1 , while MICs of aerial parts essential oil varied from 4.0 to 30.0 mg mL -1 . The obtained results contribute to the knowledge of antimicrobial properties of T. speciosa, which support traditional uses underground parts of the plant.
PB  - Belgrade:  Institute for Medicinal Plants Research "Dr. Josif Pančić"
T2  - Lekovite sirovine
T1  - GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg
IS  - 41
DO  - 10.5937/leksir2141035C
SP  - 35
EP  - 40
ER  - 

@article{
author = "Čilović-Kozarević, Ermina and Šarić-Kundalić, Broza and Ibišević, Merima and Horozić, Emir and Glamočlija, Jasmina and Soković, Marina and Arsenijević, Jelena and Maksimović, Zoran",
year = "2021",
abstract = "Telekia speciosa (Schreb.) Baumg., Asteraceae, is widespread in Eastern and Central Europe and the Balkan Peninsula. Previous phytochemical investigations have revealed T. speciosa as a rich source of sesquiterpene lactone -isoalantolactone, especially in its underground parts. The aim of the present study was to analyze the essential oils from aerial and underground parts of T. speciosa and investigate their antimicrobial activity. Chemical composition of essential oils was determined by GC-FID/MS method leading to the identification of 67 compounds in total, with 15.77 % oxygenated monoterpenes, 7.77 % sesquiterpene hydrocarbons, 49.14 % oxygenated sesquiterpenes, and 12.37 % other compounds from aerial parts, and 3.80 % oxygenated monoterpenes, 3.13 % sesquiterpene hydrocarbons, 90.33 % oxygenated sesquiterpenes from underground parts essential oil. The main components from aerial parts were (E)-nerolidol (11.54 %) and caryophyllene oxide (10.54 %), while isoalantolactone was the predominant component from essential oil underground parts (83.41 %). The minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration of the essential oils were evaluated against six strains of bacteria and two strains of fungus using in vitro microdilution method. Both oils presented antimicrobial properties against pathogens Staphylococcus aureus, Bacilus cereus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. Inhibition of growth of tested microorganisms by T. speciosa underground parts essential oil was achieved with MICs ranging from 1.0 to 11.0 mg mL -1 , while MICs of aerial parts essential oil varied from 4.0 to 30.0 mg mL -1 . The obtained results contribute to the knowledge of antimicrobial properties of T. speciosa, which support traditional uses underground parts of the plant.",
publisher = "Belgrade:  Institute for Medicinal Plants Research "Dr. Josif Pančić"",
journal = "Lekovite sirovine",
title = "GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg",
number = "41",
doi = "10.5937/leksir2141035C",
pages = "35-40"
}

Čilović-Kozarević, E., Šarić-Kundalić, B., Ibišević, M., Horozić, E., Glamočlija, J., Soković, M., Arsenijević, J.,& Maksimović, Z.. (2021). GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg. in Lekovite sirovine
Belgrade:  Institute for Medicinal Plants Research "Dr. Josif Pančić".(41), 35-40.
https://doi.org/10.5937/leksir2141035C

Čilović-Kozarević E, Šarić-Kundalić B, Ibišević M, Horozić E, Glamočlija J, Soković M, Arsenijević J, Maksimović Z. GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg. in Lekovite sirovine. 2021;(41):35-40.
doi:10.5937/leksir2141035C .

Čilović-Kozarević, Ermina, Šarić-Kundalić, Broza, Ibišević, Merima, Horozić, Emir, Glamočlija, Jasmina, Soković, Marina, Arsenijević, Jelena, Maksimović, Zoran, "GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg" in Lekovite sirovine, no. 41 (2021):35-40,
https://doi.org/10.5937/leksir2141035C . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Zivković, Jelena
AU  - Soković, Marina
AU  - Ferreira, Isabel CFR
AU  - Janković, Teodora
AU  - Maksimović, Zoran A
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1017
AB  - This study was designed to evaluate the antibacterial activity of the Veronica montana L. water extract and its main phenolic compound, protocatechuic acid. The antibacterial activity was determined by microdilution assay against six strains of Gram-positive and Gram-negative bacteria. Listeria monocytogenes was the most sensitive of the tested bacterial species. Antibacterial preserving properties of protocatechuic acid were also evaluated after its incorporation in cream cheese, using L. monocytogenes as commonly cheese contaminant. The compound successfully inhibited L monocytogenes development in cream cheese, at room temperature and in refrigerator (25 degrees C and 4 degrees C, respectively), after 3 days of inoculation. Sensory evaluation was carried out in order to validate the mentioned food system. A possible mode of action of the tested compound towards bacterial cells was assessed and appears to be direct lysis of pathogenic cytoplasmic membrane. Prediction of pharmacokinetic properties was also performed using computational analyzes. The obtained results can serve as an important platform for the development of effective natural preservatives. (C) 2013 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system
IS  - null
VL  - 55
SP  - 39
EP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1017
ER  - 

@article{
author = "Stojković, Dejan and Glamočlija, Jasmina and Zivković, Jelena and Soković, Marina and Ferreira, Isabel CFR and Janković, Teodora and Maksimović, Zoran A",
year = "2013",
abstract = "This study was designed to evaluate the antibacterial activity of the Veronica montana L. water extract and its main phenolic compound, protocatechuic acid. The antibacterial activity was determined by microdilution assay against six strains of Gram-positive and Gram-negative bacteria. Listeria monocytogenes was the most sensitive of the tested bacterial species. Antibacterial preserving properties of protocatechuic acid were also evaluated after its incorporation in cream cheese, using L. monocytogenes as commonly cheese contaminant. The compound successfully inhibited L monocytogenes development in cream cheese, at room temperature and in refrigerator (25 degrees C and 4 degrees C, respectively), after 3 days of inoculation. Sensory evaluation was carried out in order to validate the mentioned food system. A possible mode of action of the tested compound towards bacterial cells was assessed and appears to be direct lysis of pathogenic cytoplasmic membrane. Prediction of pharmacokinetic properties was also performed using computational analyzes. The obtained results can serve as an important platform for the development of effective natural preservatives. (C) 2013 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system",
number = "null",
volume = "55",
pages = "39-213",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1017"
}

Stojković, D., Glamočlija, J., Zivković, J., Soković, M., Ferreira, I. C., Janković, T.,& Maksimović, Z. A.. (2013). Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system. in Food and Chemical Toxicology, 55(null), 39-213.
https://hdl.handle.net/21.15107/rcub_ibiss_1017

Stojković D, Glamočlija J, Zivković J, Soković M, Ferreira IC, Janković T, Maksimović ZA. Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system. in Food and Chemical Toxicology. 2013;55(null):39-213.
https://hdl.handle.net/21.15107/rcub_ibiss_1017 .

Stojković, Dejan, Glamočlija, Jasmina, Zivković, Jelena, Soković, Marina, Ferreira, Isabel CFR, Janković, Teodora, Maksimović, Zoran A, "Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system" in Food and Chemical Toxicology, 55, no. null (2013):39-213,
https://hdl.handle.net/21.15107/rcub_ibiss_1017 .