TY  - JOUR
AU  - Petropoulos, Spyridon A
AU  - Taofiq, Oludemi
AU  - Fernandes, Ângela
AU  - Tzortzakis, Nikos
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel CFR
PY  - 2019
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.9881
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3445
AB  - BACKGROUND The scarcity of irrigation water is severely affecting global crop production. In this context, biostimulants are increasingly used as alternatives means against abiotic stress conditions. In this study, phenolic compounds composition and bioactive properties of common bean (Phaseolus vulgaris L.) plants grown under water stress conditions and biostimulants application were investigated. RESULTS Sixteen individual phenolic compounds were detected in both pods and seeds with a notable difference in their compositional profile. A significant effect on phenolic compounds content and composition was also observed for the biostimulants tested. Regarding the antibacterial activity, pods of the second harvest and seed extracts showed significant efficacy against Bacillus cereus, especially in water-stressed plants, where all biostimulant treatments were more effective than positive controls. Moreover, all biostimulant treatments for seed extracts of water-stressed plants were more effective against Staphylococcus aureus compared with ampicillin, whereas streptomycin showed the best results. Extracts from pods of the second harvest from normally irrigated plants showed the best results against the fungi tested, except for Penicillium verrucosum var. cyclopium. Finally, no significant cytotoxic effects were detected. CONCLUSION In conclusion, the biostimulants tested increased total phenolic compounds content compared with control treatment, especially in pods of the first harvest and seeds of water-stressed plants. Moreover, bioactive properties showed a varied response in regard to irrigation and biostimulant treatment. Therefore, biostimulants can be considered as a useful means towards increasing phenolic compounds content, and they may also affect the antimicrobial properties of pods and seeds extracts. © 2019 Society of Chemical Industry.
T2  - Journal of the Science of Food and Agriculture
T1  - Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.
DO  - 10.1002/jsfa.9881
ER  - 

@article{
author = "Petropoulos, Spyridon A and Taofiq, Oludemi and Fernandes, Ângela and Tzortzakis, Nikos and Ćirić, Ana and Soković, Marina and Barros, Lillian and Ferreira, Isabel CFR",
year = "2019",
abstract = "BACKGROUND The scarcity of irrigation water is severely affecting global crop production. In this context, biostimulants are increasingly used as alternatives means against abiotic stress conditions. In this study, phenolic compounds composition and bioactive properties of common bean (Phaseolus vulgaris L.) plants grown under water stress conditions and biostimulants application were investigated. RESULTS Sixteen individual phenolic compounds were detected in both pods and seeds with a notable difference in their compositional profile. A significant effect on phenolic compounds content and composition was also observed for the biostimulants tested. Regarding the antibacterial activity, pods of the second harvest and seed extracts showed significant efficacy against Bacillus cereus, especially in water-stressed plants, where all biostimulant treatments were more effective than positive controls. Moreover, all biostimulant treatments for seed extracts of water-stressed plants were more effective against Staphylococcus aureus compared with ampicillin, whereas streptomycin showed the best results. Extracts from pods of the second harvest from normally irrigated plants showed the best results against the fungi tested, except for Penicillium verrucosum var. cyclopium. Finally, no significant cytotoxic effects were detected. CONCLUSION In conclusion, the biostimulants tested increased total phenolic compounds content compared with control treatment, especially in pods of the first harvest and seeds of water-stressed plants. Moreover, bioactive properties showed a varied response in regard to irrigation and biostimulant treatment. Therefore, biostimulants can be considered as a useful means towards increasing phenolic compounds content, and they may also affect the antimicrobial properties of pods and seeds extracts. © 2019 Society of Chemical Industry.",
journal = "Journal of the Science of Food and Agriculture",
title = "Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.",
doi = "10.1002/jsfa.9881"
}

Petropoulos, S. A., Taofiq, O., Fernandes, Â., Tzortzakis, N., Ćirić, A., Soković, M., Barros, L.,& Ferreira, I. C.. (2019). Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.. in Journal of the Science of Food and Agriculture.
https://doi.org/10.1002/jsfa.9881

Petropoulos SA, Taofiq O, Fernandes Â, Tzortzakis N, Ćirić A, Soković M, Barros L, Ferreira IC. Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect.. in Journal of the Science of Food and Agriculture. 2019;.
doi:10.1002/jsfa.9881 .

Petropoulos, Spyridon A, Taofiq, Oludemi, Fernandes, Ângela, Tzortzakis, Nikos, Ćirić, Ana, Soković, Marina, Barros, Lillian, Ferreira, Isabel CFR, "Bioactive properties of greenhouse-cultivated green beans (Phaseolus vulgaris L.) under biostimulants and water-stress effect." in Journal of the Science of Food and Agriculture (2019),
https://doi.org/10.1002/jsfa.9881 . .

TY  - JOUR
AU  - Vieira, Vanessa
AU  - Fernandes, Ângela
AU  - Barros, Lillian
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Stojković, Dejan
AU  - Martins, Anabela
AU  - Soković, Marina
AU  - Ferreira, Isabel Cfr
PY  - 2016
UR  - http://doi.wiley.com/10.1002/jsfa.7063
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2987
AB  - BACKGROUND: Morchella conica Pers. is a species of fungus that belongs to the Morchellaceae family and was studied in order to obtain more information about this species, by comparing Portuguese and Serbian wild samples. Free sugars, fatty acids, tocopherols, organic and phenolic acids were analysed by chromatographic techniques. M. conica methanolic extracts were tested regarding antioxidant and antimicrobial properties. The absence of hepatotoxicity was confirmed in porcine liver primary cells. RESULTS: The nutrition value varied between the two samples and the Serbian one gave the highest energy contribution and content in sugars, polyunsaturated fatty acids and phenolic compounds, while the Portuguese sample was richer in saturated and monounsaturated fatty acids, tocopherols and organic acids. In general, the Portuguese sample revealed the highest antioxidant potential (except for lipid peroxidation inhibition), while the Serbian one displayed higher antimicrobial activity. None of the samples revealed toxicity towards liver cells. CONCLUSION: The present study provides new data concerning chemical characterization and bioactivity of Morchella conica Pers. © 2014 Society of Chemical Industry.
PB  - John Wiley and Sons Ltd
T2  - Journal of the Science of Food and Agriculture
T1  - Wild Morchella conica Pers. from different origins: a comparative study of nutritional and bioactive properties.
IS  - 1
VL  - 96
DO  - 10.1002/jsfa.7063
SP  - 90
EP  - 98
ER  - 

@article{
author = "Vieira, Vanessa and Fernandes, Ângela and Barros, Lillian and Glamočlija, Jasmina and Ćirić, Ana and Stojković, Dejan and Martins, Anabela and Soković, Marina and Ferreira, Isabel Cfr",
year = "2016",
abstract = "BACKGROUND: Morchella conica Pers. is a species of fungus that belongs to the Morchellaceae family and was studied in order to obtain more information about this species, by comparing Portuguese and Serbian wild samples. Free sugars, fatty acids, tocopherols, organic and phenolic acids were analysed by chromatographic techniques. M. conica methanolic extracts were tested regarding antioxidant and antimicrobial properties. The absence of hepatotoxicity was confirmed in porcine liver primary cells. RESULTS: The nutrition value varied between the two samples and the Serbian one gave the highest energy contribution and content in sugars, polyunsaturated fatty acids and phenolic compounds, while the Portuguese sample was richer in saturated and monounsaturated fatty acids, tocopherols and organic acids. In general, the Portuguese sample revealed the highest antioxidant potential (except for lipid peroxidation inhibition), while the Serbian one displayed higher antimicrobial activity. None of the samples revealed toxicity towards liver cells. CONCLUSION: The present study provides new data concerning chemical characterization and bioactivity of Morchella conica Pers. © 2014 Society of Chemical Industry.",
publisher = "John Wiley and Sons Ltd",
journal = "Journal of the Science of Food and Agriculture",
title = "Wild Morchella conica Pers. from different origins: a comparative study of nutritional and bioactive properties.",
number = "1",
volume = "96",
doi = "10.1002/jsfa.7063",
pages = "90-98"
}

Vieira, V., Fernandes, Â., Barros, L., Glamočlija, J., Ćirić, A., Stojković, D., Martins, A., Soković, M.,& Ferreira, I. C.. (2016). Wild Morchella conica Pers. from different origins: a comparative study of nutritional and bioactive properties.. in Journal of the Science of Food and Agriculture
John Wiley and Sons Ltd., 96(1), 90-98.
https://doi.org/10.1002/jsfa.7063

Vieira V, Fernandes Â, Barros L, Glamočlija J, Ćirić A, Stojković D, Martins A, Soković M, Ferreira IC. Wild Morchella conica Pers. from different origins: a comparative study of nutritional and bioactive properties.. in Journal of the Science of Food and Agriculture. 2016;96(1):90-98.
doi:10.1002/jsfa.7063 .

Vieira, Vanessa, Fernandes, Ângela, Barros, Lillian, Glamočlija, Jasmina, Ćirić, Ana, Stojković, Dejan, Martins, Anabela, Soković, Marina, Ferreira, Isabel Cfr, "Wild Morchella conica Pers. from different origins: a comparative study of nutritional and bioactive properties." in Journal of the Science of Food and Agriculture, 96, no. 1 (2016):90-98,
https://doi.org/10.1002/jsfa.7063 . .

TY  - JOUR
AU  - Soković, Marina
AU  - Rashed, Khaled N
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Calhelha, Ricardo C
AU  - Ferreira, Isabel CFR
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1047
AB  - The hydromethanolic extract of Sapindus saponaria L. aerial parts was investigated for antimicrobial activity (against several Gram-positive and Gram-negative bacteria and fungi) and capacity to inhibit the growth of different human tumor cell lines as also nontumor liver cells. The evaluated extract was further characterized in terms of phytochemicals using UV, H-1-NMR, C-13-NMR, and MS spectroscopic tools. The extract has shown a significant antimicrobial activity on all tested bacterial and fungal species. The best activity was achieved against Bacillus cereus and Staphylococcus aureus among bacteria and against all three Penicillium species tested. It also revealed cytotoxicity against human colon (HCT-15), cervical (HeLa), breast (MCF-7), and lung (NCI-H460) carcinoma cell lines, with HeLa being the most susceptible tumor cell line. The extract was not toxic for nontumor liver cells. Chromatographic separation of the extract resulted in the isolation and identification of stigmasterol, oleanolic acid, luteolin, luteolin 8-C-beta-glucoside (orientin), luteolin 6-C-beta-glucoside (isoorientin), luteolin 7-O-beta-glucuronide, and rutin. The results of the present findings may be useful for the discovery of novel antitumor and antimicrobial agents from plant origin.
T2  - Biomed Research International
T1  - Antimicrobial Activity, Growth Inhibition of Human Tumour Cell Lines, and Phytochemical Characterization of the Hydromethanolic Extract Obtained from Sapindus saponaria L. Aerial Parts
IS  - null
VL  - null
SP  - 361
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1047
ER  - 

@article{
author = "Soković, Marina and Rashed, Khaled N and Glamočlija, Jasmina and Ćirić, Ana and Calhelha, Ricardo C and Ferreira, Isabel CFR",
year = "2013",
abstract = "The hydromethanolic extract of Sapindus saponaria L. aerial parts was investigated for antimicrobial activity (against several Gram-positive and Gram-negative bacteria and fungi) and capacity to inhibit the growth of different human tumor cell lines as also nontumor liver cells. The evaluated extract was further characterized in terms of phytochemicals using UV, H-1-NMR, C-13-NMR, and MS spectroscopic tools. The extract has shown a significant antimicrobial activity on all tested bacterial and fungal species. The best activity was achieved against Bacillus cereus and Staphylococcus aureus among bacteria and against all three Penicillium species tested. It also revealed cytotoxicity against human colon (HCT-15), cervical (HeLa), breast (MCF-7), and lung (NCI-H460) carcinoma cell lines, with HeLa being the most susceptible tumor cell line. The extract was not toxic for nontumor liver cells. Chromatographic separation of the extract resulted in the isolation and identification of stigmasterol, oleanolic acid, luteolin, luteolin 8-C-beta-glucoside (orientin), luteolin 6-C-beta-glucoside (isoorientin), luteolin 7-O-beta-glucuronide, and rutin. The results of the present findings may be useful for the discovery of novel antitumor and antimicrobial agents from plant origin.",
journal = "Biomed Research International",
title = "Antimicrobial Activity, Growth Inhibition of Human Tumour Cell Lines, and Phytochemical Characterization of the Hydromethanolic Extract Obtained from Sapindus saponaria L. Aerial Parts",
number = "null",
volume = "null",
pages = "361-na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1047"
}

Soković, M., Rashed, K. N., Glamočlija, J., Ćirić, A., Calhelha, R. C.,& Ferreira, I. C.. (2013). Antimicrobial Activity, Growth Inhibition of Human Tumour Cell Lines, and Phytochemical Characterization of the Hydromethanolic Extract Obtained from Sapindus saponaria L. Aerial Parts. in Biomed Research International, null(null), 361-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1047

Soković M, Rashed KN, Glamočlija J, Ćirić A, Calhelha RC, Ferreira IC. Antimicrobial Activity, Growth Inhibition of Human Tumour Cell Lines, and Phytochemical Characterization of the Hydromethanolic Extract Obtained from Sapindus saponaria L. Aerial Parts. in Biomed Research International. 2013;null(null):361-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1047 .

Soković, Marina, Rashed, Khaled N, Glamočlija, Jasmina, Ćirić, Ana, Calhelha, Ricardo C, Ferreira, Isabel CFR, "Antimicrobial Activity, Growth Inhibition of Human Tumour Cell Lines, and Phytochemical Characterization of the Hydromethanolic Extract Obtained from Sapindus saponaria L. Aerial Parts" in Biomed Research International, null, no. null (2013):361-na,
https://hdl.handle.net/21.15107/rcub_ibiss_1047 .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Zivković, Jelena
AU  - Soković, Marina
AU  - Ferreira, Isabel CFR
AU  - Janković, Teodora
AU  - Maksimović, Zoran A
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1017
AB  - This study was designed to evaluate the antibacterial activity of the Veronica montana L. water extract and its main phenolic compound, protocatechuic acid. The antibacterial activity was determined by microdilution assay against six strains of Gram-positive and Gram-negative bacteria. Listeria monocytogenes was the most sensitive of the tested bacterial species. Antibacterial preserving properties of protocatechuic acid were also evaluated after its incorporation in cream cheese, using L. monocytogenes as commonly cheese contaminant. The compound successfully inhibited L monocytogenes development in cream cheese, at room temperature and in refrigerator (25 degrees C and 4 degrees C, respectively), after 3 days of inoculation. Sensory evaluation was carried out in order to validate the mentioned food system. A possible mode of action of the tested compound towards bacterial cells was assessed and appears to be direct lysis of pathogenic cytoplasmic membrane. Prediction of pharmacokinetic properties was also performed using computational analyzes. The obtained results can serve as an important platform for the development of effective natural preservatives. (C) 2013 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system
IS  - null
VL  - 55
SP  - 39
EP  - 213
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1017
ER  - 

@article{
author = "Stojković, Dejan and Glamočlija, Jasmina and Zivković, Jelena and Soković, Marina and Ferreira, Isabel CFR and Janković, Teodora and Maksimović, Zoran A",
year = "2013",
abstract = "This study was designed to evaluate the antibacterial activity of the Veronica montana L. water extract and its main phenolic compound, protocatechuic acid. The antibacterial activity was determined by microdilution assay against six strains of Gram-positive and Gram-negative bacteria. Listeria monocytogenes was the most sensitive of the tested bacterial species. Antibacterial preserving properties of protocatechuic acid were also evaluated after its incorporation in cream cheese, using L. monocytogenes as commonly cheese contaminant. The compound successfully inhibited L monocytogenes development in cream cheese, at room temperature and in refrigerator (25 degrees C and 4 degrees C, respectively), after 3 days of inoculation. Sensory evaluation was carried out in order to validate the mentioned food system. A possible mode of action of the tested compound towards bacterial cells was assessed and appears to be direct lysis of pathogenic cytoplasmic membrane. Prediction of pharmacokinetic properties was also performed using computational analyzes. The obtained results can serve as an important platform for the development of effective natural preservatives. (C) 2013 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system",
number = "null",
volume = "55",
pages = "39-213",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1017"
}

Stojković, D., Glamočlija, J., Zivković, J., Soković, M., Ferreira, I. C., Janković, T.,& Maksimović, Z. A.. (2013). Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system. in Food and Chemical Toxicology, 55(null), 39-213.
https://hdl.handle.net/21.15107/rcub_ibiss_1017

Stojković D, Glamočlija J, Zivković J, Soković M, Ferreira IC, Janković T, Maksimović ZA. Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system. in Food and Chemical Toxicology. 2013;55(null):39-213.
https://hdl.handle.net/21.15107/rcub_ibiss_1017 .

Stojković, Dejan, Glamočlija, Jasmina, Zivković, Jelena, Soković, Marina, Ferreira, Isabel CFR, Janković, Teodora, Maksimović, Zoran A, "Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system" in Food and Chemical Toxicology, 55, no. null (2013):39-213,
https://hdl.handle.net/21.15107/rcub_ibiss_1017 .

TY  - JOUR
AU  - Heleno, Sandrina A
AU  - Stojković, Dejan
AU  - Barros, Lillian
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Martins, Anabela
AU  - Queiroz, Maria Joao RP
AU  - Ferreira, Isabel CFR
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1029
AB  - A comparative study on chemical composition (nutritional value, primary and secondary metabolites), antioxidant properties (scavenging activity, reducing power and inhibition of lipid peroxidation), and antimicrobial activity (antibacterial and demelanizing properties) of two samples of Morchella esculenta (morel) from different countries (Portugal and Serbia) was performed. This species was chosen for being one of the most highly prized edible mushrooms in the world. Both samples are rich in carbohydrates (including free sugars) and proteins, and contain several bioactive compounds such as organic acids, phenolic compounds and tocopherols. Polyunsaturated fatty adds were the most abundant compounds followed by mono or saturated fatty acids. Sample from Portugal (SP) gave higher radical scavenging activity and reducing power, while sample from Serbia (SS) showed higher lipid peroxidation inhibition. Both samples gave antibacterial activity against five bacteria (in some cases even better than standard antibiotics) and demelanizing activity against four micromycetes, showing SS higher activities. As far as we know, this is the first study reporting chemical compounds and bioactivity of morel samples from Portugal and Serbia. Furthermore, a novel method for evaluation of demelanizing activity was presented. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Food Research International
T1  - A comparative study of chemical composition, antioxidant and antimicrobial properties of Morchella esculenta (L.) Pers. from Portugal and Serbia
IS  - 1
VL  - 51
SP  - 29
EP  - 243
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1029
ER  - 

@article{
author = "Heleno, Sandrina A and Stojković, Dejan and Barros, Lillian and Glamočlija, Jasmina and Soković, Marina and Martins, Anabela and Queiroz, Maria Joao RP and Ferreira, Isabel CFR",
year = "2013",
abstract = "A comparative study on chemical composition (nutritional value, primary and secondary metabolites), antioxidant properties (scavenging activity, reducing power and inhibition of lipid peroxidation), and antimicrobial activity (antibacterial and demelanizing properties) of two samples of Morchella esculenta (morel) from different countries (Portugal and Serbia) was performed. This species was chosen for being one of the most highly prized edible mushrooms in the world. Both samples are rich in carbohydrates (including free sugars) and proteins, and contain several bioactive compounds such as organic acids, phenolic compounds and tocopherols. Polyunsaturated fatty adds were the most abundant compounds followed by mono or saturated fatty acids. Sample from Portugal (SP) gave higher radical scavenging activity and reducing power, while sample from Serbia (SS) showed higher lipid peroxidation inhibition. Both samples gave antibacterial activity against five bacteria (in some cases even better than standard antibiotics) and demelanizing activity against four micromycetes, showing SS higher activities. As far as we know, this is the first study reporting chemical compounds and bioactivity of morel samples from Portugal and Serbia. Furthermore, a novel method for evaluation of demelanizing activity was presented. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Food Research International",
title = "A comparative study of chemical composition, antioxidant and antimicrobial properties of Morchella esculenta (L.) Pers. from Portugal and Serbia",
number = "1",
volume = "51",
pages = "29-243",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1029"
}

Heleno, S. A., Stojković, D., Barros, L., Glamočlija, J., Soković, M., Martins, A., Queiroz, M. J. R.,& Ferreira, I. C.. (2013). A comparative study of chemical composition, antioxidant and antimicrobial properties of Morchella esculenta (L.) Pers. from Portugal and Serbia. in Food Research International, 51(1), 29-243.
https://hdl.handle.net/21.15107/rcub_ibiss_1029

Heleno SA, Stojković D, Barros L, Glamočlija J, Soković M, Martins A, Queiroz MJR, Ferreira IC. A comparative study of chemical composition, antioxidant and antimicrobial properties of Morchella esculenta (L.) Pers. from Portugal and Serbia. in Food Research International. 2013;51(1):29-243.
https://hdl.handle.net/21.15107/rcub_ibiss_1029 .

Heleno, Sandrina A, Stojković, Dejan, Barros, Lillian, Glamočlija, Jasmina, Soković, Marina, Martins, Anabela, Queiroz, Maria Joao RP, Ferreira, Isabel CFR, "A comparative study of chemical composition, antioxidant and antimicrobial properties of Morchella esculenta (L.) Pers. from Portugal and Serbia" in Food Research International, 51, no. 1 (2013):29-243,
https://hdl.handle.net/21.15107/rcub_ibiss_1029 .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Reis, Filipa S
AU  - Barros, Lillian
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - van Griensven, Leo JLD
AU  - Soković, Marina
AU  - Ferreira, Isabel CFR
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/970
AB  - Mushrooms have been reported as sources of biomolecules with various potential. Coprinus comatus was studied to obtain information about this species, comparing cultivated and wild samples. Free sugars, fatty acids, tocopherols, organic acids and phenolic acids were analyzed by chromatographic techniques coupled to different detectors. C. comatus methanolic extract was tested for its antioxidant potential (reducing power, radical scavenging activity and lipid peroxidation inhibition) and antimicrobial properties (tested towards Gram positive and negative bacteria, and microfungi). The toxicity for liver cells was tested in porcine liver primary cells. Both studied samples revealed similar nutritional value and energy contribution. The cultivated sample revealed the highest content in free sugars, monounsaturated fatty acids and tocopherols, while the wild mushroom was richer in saturated and polyunsaturated fatty acids, organic acids and phenolic compounds. The cultivated species also revealed the highest antioxidant potential and antimicrobial activity (with exception towards Gram negative bacteria and Aspergillus ochraceus). Both species revealed no toxicity towards porcine liver cells. The present study proved that cultivated and wild mushrooms from the same species could be excellent options as food and as sources of nutritional and bioactive compounds. Furthermore, differences in wild and cultivated samples were comparatively investigated for the first time. (c) 2013 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Nutrients and non-nutrients composition and bioactivity of wild and cultivated Coprinus comatus (OFMull.) Pers.
IS  - null
VL  - 59
SP  - 139
EP  - 296
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_970
ER  - 

@article{
author = "Stojković, Dejan and Reis, Filipa S and Barros, Lillian and Glamočlija, Jasmina and Ćirić, Ana and van Griensven, Leo JLD and Soković, Marina and Ferreira, Isabel CFR",
year = "2013",
abstract = "Mushrooms have been reported as sources of biomolecules with various potential. Coprinus comatus was studied to obtain information about this species, comparing cultivated and wild samples. Free sugars, fatty acids, tocopherols, organic acids and phenolic acids were analyzed by chromatographic techniques coupled to different detectors. C. comatus methanolic extract was tested for its antioxidant potential (reducing power, radical scavenging activity and lipid peroxidation inhibition) and antimicrobial properties (tested towards Gram positive and negative bacteria, and microfungi). The toxicity for liver cells was tested in porcine liver primary cells. Both studied samples revealed similar nutritional value and energy contribution. The cultivated sample revealed the highest content in free sugars, monounsaturated fatty acids and tocopherols, while the wild mushroom was richer in saturated and polyunsaturated fatty acids, organic acids and phenolic compounds. The cultivated species also revealed the highest antioxidant potential and antimicrobial activity (with exception towards Gram negative bacteria and Aspergillus ochraceus). Both species revealed no toxicity towards porcine liver cells. The present study proved that cultivated and wild mushrooms from the same species could be excellent options as food and as sources of nutritional and bioactive compounds. Furthermore, differences in wild and cultivated samples were comparatively investigated for the first time. (c) 2013 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Nutrients and non-nutrients composition and bioactivity of wild and cultivated Coprinus comatus (OFMull.) Pers.",
number = "null",
volume = "59",
pages = "139-296",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_970"
}

Stojković, D., Reis, F. S., Barros, L., Glamočlija, J., Ćirić, A., van Griensven, L. J., Soković, M.,& Ferreira, I. C.. (2013). Nutrients and non-nutrients composition and bioactivity of wild and cultivated Coprinus comatus (OFMull.) Pers.. in Food and Chemical Toxicology, 59(null), 139-296.
https://hdl.handle.net/21.15107/rcub_ibiss_970

Stojković D, Reis FS, Barros L, Glamočlija J, Ćirić A, van Griensven LJ, Soković M, Ferreira IC. Nutrients and non-nutrients composition and bioactivity of wild and cultivated Coprinus comatus (OFMull.) Pers.. in Food and Chemical Toxicology. 2013;59(null):139-296.
https://hdl.handle.net/21.15107/rcub_ibiss_970 .

Stojković, Dejan, Reis, Filipa S, Barros, Lillian, Glamočlija, Jasmina, Ćirić, Ana, van Griensven, Leo JLD, Soković, Marina, Ferreira, Isabel CFR, "Nutrients and non-nutrients composition and bioactivity of wild and cultivated Coprinus comatus (OFMull.) Pers." in Food and Chemical Toxicology, 59, no. null (2013):139-296,
https://hdl.handle.net/21.15107/rcub_ibiss_970 .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Davidović, Slobodan
AU  - Zivković, Jelena
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Stevanović, Milena
AU  - Ferreira, Isabel CFR
AU  - Soković, Marina
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/939
AB  - Morchella esculenta (L.) Pers. methanolic extracts, obtained from fruiting bodies growing wild in Serbia and Portugal, were screened for their antimutagenic properties and compared to protocatechuic acid, previously identified in both species. Salmonella typhimurium TA100 reversion assay was used for the antimutagenic properties. Methanolic extracts expressed important antimutagenic potency towards S. typhimurium, which was documented by index of antimutagenicity (I). A sample from Serbia expressed slightly higher antimutagenic properties with an inhibition rate of 58.7%. A sample from Portugal gave an inhibition rate of 51.7%. Protocatechuic acid had an inhibition rate I of his(+) revertants of 72.4%. Cell viability in the presence of extracts was also documented. M. esculenta samples from Serbia and Portugal possessed novel biological potential for the studied species, as well as its phenolic compound - protocatechuic acid, identified in both samples. Genotoxic effect, regarding mitotic index and chromosomal aberration score, was also assessed by using the Allium cepa L. assay. Protocatechuic acid showed the most significant decrease in mitotic index, as well as decrease in chromosomal aberration score.
T2  - Frontiers in Life Science
T1  - Comparative evaluation of antimutagenic and antimitotic effects of Morchella esculenta extracts and protocatechuic acid
IS  - 3-4
VL  - 7
SP  - 1405
EP  - 223
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_939
ER  - 

@article{
author = "Stojković, Dejan and Davidović, Slobodan and Zivković, Jelena and Glamočlija, Jasmina and Ćirić, Ana and Stevanović, Milena and Ferreira, Isabel CFR and Soković, Marina",
year = "2013",
abstract = "Morchella esculenta (L.) Pers. methanolic extracts, obtained from fruiting bodies growing wild in Serbia and Portugal, were screened for their antimutagenic properties and compared to protocatechuic acid, previously identified in both species. Salmonella typhimurium TA100 reversion assay was used for the antimutagenic properties. Methanolic extracts expressed important antimutagenic potency towards S. typhimurium, which was documented by index of antimutagenicity (I). A sample from Serbia expressed slightly higher antimutagenic properties with an inhibition rate of 58.7%. A sample from Portugal gave an inhibition rate of 51.7%. Protocatechuic acid had an inhibition rate I of his(+) revertants of 72.4%. Cell viability in the presence of extracts was also documented. M. esculenta samples from Serbia and Portugal possessed novel biological potential for the studied species, as well as its phenolic compound - protocatechuic acid, identified in both samples. Genotoxic effect, regarding mitotic index and chromosomal aberration score, was also assessed by using the Allium cepa L. assay. Protocatechuic acid showed the most significant decrease in mitotic index, as well as decrease in chromosomal aberration score.",
journal = "Frontiers in Life Science",
title = "Comparative evaluation of antimutagenic and antimitotic effects of Morchella esculenta extracts and protocatechuic acid",
number = "3-4",
volume = "7",
pages = "1405-223",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_939"
}

Stojković, D., Davidović, S., Zivković, J., Glamočlija, J., Ćirić, A., Stevanović, M., Ferreira, I. C.,& Soković, M.. (2013). Comparative evaluation of antimutagenic and antimitotic effects of Morchella esculenta extracts and protocatechuic acid. in Frontiers in Life Science, 7(3-4), 1405-223.
https://hdl.handle.net/21.15107/rcub_ibiss_939

Stojković D, Davidović S, Zivković J, Glamočlija J, Ćirić A, Stevanović M, Ferreira IC, Soković M. Comparative evaluation of antimutagenic and antimitotic effects of Morchella esculenta extracts and protocatechuic acid. in Frontiers in Life Science. 2013;7(3-4):1405-223.
https://hdl.handle.net/21.15107/rcub_ibiss_939 .

Stojković, Dejan, Davidović, Slobodan, Zivković, Jelena, Glamočlija, Jasmina, Ćirić, Ana, Stevanović, Milena, Ferreira, Isabel CFR, Soković, Marina, "Comparative evaluation of antimutagenic and antimitotic effects of Morchella esculenta extracts and protocatechuic acid" in Frontiers in Life Science, 7, no. 3-4 (2013):1405-223,
https://hdl.handle.net/21.15107/rcub_ibiss_939 .

TY  - JOUR
AU  - Reis, Filipa S
AU  - Barros, Lillian
AU  - Calhelha, Ricardo C
AU  - Ćirić, Ana
AU  - van Griensven, Leo JLD
AU  - Soković, Marina
AU  - Ferreira, Isabel CFR
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/941
AB  - Being Cordyceps militaris (L.) Link recognized as a medicinal and edible mushroom, this work intends to reveal new interesting bioactive molecules that could be isolated from this species. Hydrophilic and lipophilic compounds were analyzed by chromatographic techniques coupled to different detectors. The methanolic extract of C. militaris was tested for its antioxidant, antibacterial, antifungal and antiproliferative properties in different human tumor cell lines. Mannitol (2.01 g/100 g dw) and trehalose (24.71 g/100 g) were the free sugars found in C. militaris. Polyunsaturated fatty acids (68.87%) predominated over saturated fatty acids (23.40%) and delta-tocopherol was the only isoform of vitamin E detected (55.86 mu g/100 g). The organic acids found in this mushroom were oxalic, citric and fumaric acids (0.33, 7.97 and 0.13 g/100 g, respectively). p-Hydroxybenzoic acid was the only phenolic acid quantified in this species (0.02 mg/100 g); although cinnamic acid was also found (0.11 mg/100 g). The methanolic extract of C. militaris proved to inhibit lipid peroxidation, have reducing power and scavenge free radicals. This extract also revealed strong antibacterial and antifungal properties. Finally, the C militaris extract was able to inhibit the proliferation of MCF-7 (breast), NCI-H460 (non-small lung), HCT-15 (colon) and HeLa (cervical) human carcinoma cell lines. (C) 2013 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and antihuman tumor cell lines properties
IS  - null
VL  - 62
SP  - 1469
EP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_941
ER  - 

@article{
author = "Reis, Filipa S and Barros, Lillian and Calhelha, Ricardo C and Ćirić, Ana and van Griensven, Leo JLD and Soković, Marina and Ferreira, Isabel CFR",
year = "2013",
abstract = "Being Cordyceps militaris (L.) Link recognized as a medicinal and edible mushroom, this work intends to reveal new interesting bioactive molecules that could be isolated from this species. Hydrophilic and lipophilic compounds were analyzed by chromatographic techniques coupled to different detectors. The methanolic extract of C. militaris was tested for its antioxidant, antibacterial, antifungal and antiproliferative properties in different human tumor cell lines. Mannitol (2.01 g/100 g dw) and trehalose (24.71 g/100 g) were the free sugars found in C. militaris. Polyunsaturated fatty acids (68.87%) predominated over saturated fatty acids (23.40%) and delta-tocopherol was the only isoform of vitamin E detected (55.86 mu g/100 g). The organic acids found in this mushroom were oxalic, citric and fumaric acids (0.33, 7.97 and 0.13 g/100 g, respectively). p-Hydroxybenzoic acid was the only phenolic acid quantified in this species (0.02 mg/100 g); although cinnamic acid was also found (0.11 mg/100 g). The methanolic extract of C. militaris proved to inhibit lipid peroxidation, have reducing power and scavenge free radicals. This extract also revealed strong antibacterial and antifungal properties. Finally, the C militaris extract was able to inhibit the proliferation of MCF-7 (breast), NCI-H460 (non-small lung), HCT-15 (colon) and HeLa (cervical) human carcinoma cell lines. (C) 2013 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and antihuman tumor cell lines properties",
number = "null",
volume = "62",
pages = "1469-98",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_941"
}

Reis, F. S., Barros, L., Calhelha, R. C., Ćirić, A., van Griensven, L. J., Soković, M.,& Ferreira, I. C.. (2013). The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and antihuman tumor cell lines properties. in Food and Chemical Toxicology, 62(null), 1469-98.
https://hdl.handle.net/21.15107/rcub_ibiss_941

Reis FS, Barros L, Calhelha RC, Ćirić A, van Griensven LJ, Soković M, Ferreira IC. The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and antihuman tumor cell lines properties. in Food and Chemical Toxicology. 2013;62(null):1469-98.
https://hdl.handle.net/21.15107/rcub_ibiss_941 .

Reis, Filipa S, Barros, Lillian, Calhelha, Ricardo C, Ćirić, Ana, van Griensven, Leo JLD, Soković, Marina, Ferreira, Isabel CFR, "The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and antihuman tumor cell lines properties" in Food and Chemical Toxicology, 62, no. null (2013):1469-98,
https://hdl.handle.net/21.15107/rcub_ibiss_941 .

TY  - JOUR
AU  - Heleno, Sandrina A
AU  - Ferreira, Isabel CFR
AU  - Esteves, Ana P
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Martins, Anabela
AU  - Soković, Marina
AU  - Queiroz, Maria Joao RP
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/983
AB  - Mushroom extracts or isolated compounds may be useful in the search of new potent antimicrobial agents. Herein, it is described the synthesis of protected (acetylated) glucuronide derivatives of p-hydroxybenzoic and cinnamic acids, two compounds identified in the medicinal mushroom Ganoderma lucidum. Their antimicrobial and demelanizing activities were evaluated and compared to the parent acids and G. lucidum extract. p-Hydroxybenzoic and cinnamic acids, as also their protected glucuronide derivatives revealed high antimicrobial (antibacterial and antifungal) activity, even better than the one showed by commercial standards. Despite the variation in the order of parent acids and the protected glucuronide derivatives, their antimicrobial activity was always higher than the one revealed by the extract. Nevertheless, the extract was the only one with demelanizing activity against Aspergillus niger. The acetylated glucuronide derivatives could be deprotected to obtain glucuronide metabolites, which circulate in the human organism as products of the metabolism of the parent compounds. (C) 2013 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Antimicrobial and demelanizing activity of Ganoderma lucidum extract, p-hydroxybenzoic and cinnamic acids and their synthetic acetylated glucuronide methyl esters
IS  - null
VL  - 58
SP  - 63
EP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_983
ER  - 

@article{
author = "Heleno, Sandrina A and Ferreira, Isabel CFR and Esteves, Ana P and Ćirić, Ana and Glamočlija, Jasmina and Martins, Anabela and Soković, Marina and Queiroz, Maria Joao RP",
year = "2013",
abstract = "Mushroom extracts or isolated compounds may be useful in the search of new potent antimicrobial agents. Herein, it is described the synthesis of protected (acetylated) glucuronide derivatives of p-hydroxybenzoic and cinnamic acids, two compounds identified in the medicinal mushroom Ganoderma lucidum. Their antimicrobial and demelanizing activities were evaluated and compared to the parent acids and G. lucidum extract. p-Hydroxybenzoic and cinnamic acids, as also their protected glucuronide derivatives revealed high antimicrobial (antibacterial and antifungal) activity, even better than the one showed by commercial standards. Despite the variation in the order of parent acids and the protected glucuronide derivatives, their antimicrobial activity was always higher than the one revealed by the extract. Nevertheless, the extract was the only one with demelanizing activity against Aspergillus niger. The acetylated glucuronide derivatives could be deprotected to obtain glucuronide metabolites, which circulate in the human organism as products of the metabolism of the parent compounds. (C) 2013 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Antimicrobial and demelanizing activity of Ganoderma lucidum extract, p-hydroxybenzoic and cinnamic acids and their synthetic acetylated glucuronide methyl esters",
number = "null",
volume = "58",
pages = "63-100",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_983"
}

Heleno, S. A., Ferreira, I. C., Esteves, A. P., Ćirić, A., Glamočlija, J., Martins, A., Soković, M.,& Queiroz, M. J. R.. (2013). Antimicrobial and demelanizing activity of Ganoderma lucidum extract, p-hydroxybenzoic and cinnamic acids and their synthetic acetylated glucuronide methyl esters. in Food and Chemical Toxicology, 58(null), 63-100.
https://hdl.handle.net/21.15107/rcub_ibiss_983

Heleno SA, Ferreira IC, Esteves AP, Ćirić A, Glamočlija J, Martins A, Soković M, Queiroz MJR. Antimicrobial and demelanizing activity of Ganoderma lucidum extract, p-hydroxybenzoic and cinnamic acids and their synthetic acetylated glucuronide methyl esters. in Food and Chemical Toxicology. 2013;58(null):63-100.
https://hdl.handle.net/21.15107/rcub_ibiss_983 .

Heleno, Sandrina A, Ferreira, Isabel CFR, Esteves, Ana P, Ćirić, Ana, Glamočlija, Jasmina, Martins, Anabela, Soković, Marina, Queiroz, Maria Joao RP, "Antimicrobial and demelanizing activity of Ganoderma lucidum extract, p-hydroxybenzoic and cinnamic acids and their synthetic acetylated glucuronide methyl esters" in Food and Chemical Toxicology, 58, no. null (2013):63-100,
https://hdl.handle.net/21.15107/rcub_ibiss_983 .

TY  - JOUR
AU  - Reis, Filipa S
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Barros, Lillian
AU  - Ferreira, Isabel CFR
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1097
AB  - Agaricus bohusii Bon is an edible and prized mushroom especially common in Serbia and southern Europe. Herein, this species was chemically characterized by the evaluation of nutritional value (e.g. macromolecules, free sugars and fatty acids), bioactive compounds (e.g. tocopherols. phenolic compounds and organic acids). and antioxidant activity of its methanolic extract (e.g. scavenging activity, reducing power and inhibition of lipid peroxidation). Its antifungal preserving properties were also evaluated after the incorporation of A. bohusii extract in cream cheese, using the fungus Penicillium verucosum var. cyclopium as the source contaminant. Comparison of the sensory evaluation of cream cheese alone and enriched with A. bohusii extract was recorded. According to our findings, A. bohusii was a rich source of carbohydrates and proteins, containing gamma-tocopherol as the only isoform of tocopherols. Polyunsaturated fatty acids also predominated over mono and unsaturated fatty acids. p-Hydroxybenzoic and p-coumaric acids were the phenolic acids identified in the studied sample; two related compounds were found in higher amounts: gamma-L-glutaminyl-4-hydroxybenzene and cinnamic acid. Malic, oxalic and fumaric acids were the organic acids identified and quantified in A. bohusii. A high concentration of the total phenolics was in correlation with strong antioxidant capacity. Methanol extract successfully inhibited the development of P. verucosum var. cyclopium in cream cheese, tested at room temperature after 7 days of inoculation. Sensory evaluation showed cream cheese in combination with A. bohusii extract slightly more acceptable to panelists than cream cheese alone. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Food Research International
T1  - Chemical characterization of Agaricus bohusii, antioxidant potential and antifungal preserving properties when incorporated in cream cheese
IS  - 2
VL  - 48
SP  - 398
EP  - 626
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1097
ER  - 

@article{
author = "Reis, Filipa S and Stojković, Dejan and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Barros, Lillian and Ferreira, Isabel CFR",
year = "2012",
abstract = "Agaricus bohusii Bon is an edible and prized mushroom especially common in Serbia and southern Europe. Herein, this species was chemically characterized by the evaluation of nutritional value (e.g. macromolecules, free sugars and fatty acids), bioactive compounds (e.g. tocopherols. phenolic compounds and organic acids). and antioxidant activity of its methanolic extract (e.g. scavenging activity, reducing power and inhibition of lipid peroxidation). Its antifungal preserving properties were also evaluated after the incorporation of A. bohusii extract in cream cheese, using the fungus Penicillium verucosum var. cyclopium as the source contaminant. Comparison of the sensory evaluation of cream cheese alone and enriched with A. bohusii extract was recorded. According to our findings, A. bohusii was a rich source of carbohydrates and proteins, containing gamma-tocopherol as the only isoform of tocopherols. Polyunsaturated fatty acids also predominated over mono and unsaturated fatty acids. p-Hydroxybenzoic and p-coumaric acids were the phenolic acids identified in the studied sample; two related compounds were found in higher amounts: gamma-L-glutaminyl-4-hydroxybenzene and cinnamic acid. Malic, oxalic and fumaric acids were the organic acids identified and quantified in A. bohusii. A high concentration of the total phenolics was in correlation with strong antioxidant capacity. Methanol extract successfully inhibited the development of P. verucosum var. cyclopium in cream cheese, tested at room temperature after 7 days of inoculation. Sensory evaluation showed cream cheese in combination with A. bohusii extract slightly more acceptable to panelists than cream cheese alone. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Food Research International",
title = "Chemical characterization of Agaricus bohusii, antioxidant potential and antifungal preserving properties when incorporated in cream cheese",
number = "2",
volume = "48",
pages = "398-626",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1097"
}

Reis, F. S., Stojković, D., Soković, M., Glamočlija, J., Ćirić, A., Barros, L.,& Ferreira, I. C.. (2012). Chemical characterization of Agaricus bohusii, antioxidant potential and antifungal preserving properties when incorporated in cream cheese. in Food Research International, 48(2), 398-626.
https://hdl.handle.net/21.15107/rcub_ibiss_1097

Reis FS, Stojković D, Soković M, Glamočlija J, Ćirić A, Barros L, Ferreira IC. Chemical characterization of Agaricus bohusii, antioxidant potential and antifungal preserving properties when incorporated in cream cheese. in Food Research International. 2012;48(2):398-626.
https://hdl.handle.net/21.15107/rcub_ibiss_1097 .

Reis, Filipa S, Stojković, Dejan, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Barros, Lillian, Ferreira, Isabel CFR, "Chemical characterization of Agaricus bohusii, antioxidant potential and antifungal preserving properties when incorporated in cream cheese" in Food Research International, 48, no. 2 (2012):398-626,
https://hdl.handle.net/21.15107/rcub_ibiss_1097 .