TY  - JOUR
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Mišić, Danijela
AU  - Stanković, Vesna
AU  - Mihailović, Mirjana
AU  - Uskoković, Aleksandra
AU  - Arambašić Jovanović, Jelena
AU  - Solujić, Slavica
AU  - Mladenović, Milan
AU  - Stanković, Nevena
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2179
AB  - The objective of this work was to investigate the effects of the
   methanol extracts of Gentiana cruciata L. aerial parts (GCA) and roots
   (GCR) against carbon tetrachloride-induced liver injury in rats.
   Pretreatment with GCA and GCR, containing sweroside, swertiamarin and
   gentiopicrin in high concentrations, dose-dependently and significantly
   decreased the levels of serum transaminases, alkaline phosphatase and
   total bilirubin, whereas an increase in the level of total protein was
   found compared with the CCl4-treated group. Moreover, oral
   administration of extracts significantly enhanced antioxidant enzyme
   activities (superoxide dismutase and catalase), increased the content of
   glutathione and decreased the content of TBARS. Microscopic evaluations
   of the liver revealed CCl4-induced lesions and related toxic
   manifestations that were minimal in the liver of rats pretreated with
   extracts at the dose of 400 mg per kg b.w. The results suggest that the
   use of G. cruciata extracts has a merit as a potent candidate in
   protecting the liver against chemical induced toxicity.
PB  - The Royal Society of Chemistry
T2  - Food and Function
T1  - Hepatoprotective effects of secoiridoid-rich extracts from Gentiana
 cruciata L. against carbon tetrachloride induced liver damage in rats
IS  - 8
VL  - 5
DO  - 10.1039/c4fo00088a
SP  - 1795
EP  - 1803
ER  - 

@article{
author = "Mihailović, Vladimir and Katanić, Jelena and Mišić, Danijela and Stanković, Vesna and Mihailović, Mirjana and Uskoković, Aleksandra and Arambašić Jovanović, Jelena and Solujić, Slavica and Mladenović, Milan and Stanković, Nevena",
year = "2014",
abstract = "The objective of this work was to investigate the effects of the
   methanol extracts of Gentiana cruciata L. aerial parts (GCA) and roots
   (GCR) against carbon tetrachloride-induced liver injury in rats.
   Pretreatment with GCA and GCR, containing sweroside, swertiamarin and
   gentiopicrin in high concentrations, dose-dependently and significantly
   decreased the levels of serum transaminases, alkaline phosphatase and
   total bilirubin, whereas an increase in the level of total protein was
   found compared with the CCl4-treated group. Moreover, oral
   administration of extracts significantly enhanced antioxidant enzyme
   activities (superoxide dismutase and catalase), increased the content of
   glutathione and decreased the content of TBARS. Microscopic evaluations
   of the liver revealed CCl4-induced lesions and related toxic
   manifestations that were minimal in the liver of rats pretreated with
   extracts at the dose of 400 mg per kg b.w. The results suggest that the
   use of G. cruciata extracts has a merit as a potent candidate in
   protecting the liver against chemical induced toxicity.",
publisher = "The Royal Society of Chemistry",
journal = "Food and Function",
title = "Hepatoprotective effects of secoiridoid-rich extracts from Gentiana
 cruciata L. against carbon tetrachloride induced liver damage in rats",
number = "8",
volume = "5",
doi = "10.1039/c4fo00088a",
pages = "1795-1803"
}

Mihailović, V., Katanić, J., Mišić, D., Stanković, V., Mihailović, M., Uskoković, A., Arambašić Jovanović, J., Solujić, S., Mladenović, M.,& Stanković, N.. (2014). Hepatoprotective effects of secoiridoid-rich extracts from Gentiana
 cruciata L. against carbon tetrachloride induced liver damage in rats. in Food and Function
The Royal Society of Chemistry., 5(8), 1795-1803.
https://doi.org/10.1039/c4fo00088a

Mihailović V, Katanić J, Mišić D, Stanković V, Mihailović M, Uskoković A, Arambašić Jovanović J, Solujić S, Mladenović M, Stanković N. Hepatoprotective effects of secoiridoid-rich extracts from Gentiana
 cruciata L. against carbon tetrachloride induced liver damage in rats. in Food and Function. 2014;5(8):1795-1803.
doi:10.1039/c4fo00088a .

Mihailović, Vladimir, Katanić, Jelena, Mišić, Danijela, Stanković, Vesna, Mihailović, Mirjana, Uskoković, Aleksandra, Arambašić Jovanović, Jelena, Solujić, Slavica, Mladenović, Milan, Stanković, Nevena, "Hepatoprotective effects of secoiridoid-rich extracts from Gentiana
 cruciata L. against carbon tetrachloride induced liver damage in rats" in Food and Function, 5, no. 8 (2014):1795-1803,
https://doi.org/10.1039/c4fo00088a . .

TY  - CONF
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Bogojević, Desanka
AU  - Solujić, Slavica
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Mihailović, Mirjana
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6141
AB  - The plant Cotinus coggygria Scop. (family Anacardiaceae) is commonly used in folk medicine for the treat­ment of various illnesses, such as diarrhea, paradontosis, gastric and duodenal ulcers. Different parts of this plant have been subjected to pharmacological evaluation for their potential antihaemorragic, wound-healing, anti­inflammatory, and antimicrobial activity. The present study was undertaken to investigate the phenolic conten according to the Folin-Ciocalteu procedure, while the spectrophotometric method with aluminium chloride was used for the determination of total ftavonoids. The phenolic and flavonoid composition of extract were deter­mined by the HPLC method. Antioxidant activiy was quantitatively determined using a DPPH radical scaveng­ing assay. To examine the antigenotoxic potential using the comet assay, Wistar rats were treated with 100 mg/ kg body weight of pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The content of total phenols and ftavonoids was 3.78 mg of gallic acid/g of dry plant material and 8.29 mg of rutin/g of dry plant material, respectively. HPLC analysis showed that myricetin was the dominant compound (511.5 µg/g), while hydroxyl derivatives of cinnammic acids (chlorogenic, caffeic, coumaric, ferulic and rosmaric acid) were identified in the extract in varying amount. The neutralisation of DPPH radicals was up to 95%, while the maximum inhibition concentration is approximately 125 µg/ml. The dose of 500 mg/kg body weight of the extract, that display no genotoxic activity, and its main flavonoid compound myricetin, in equivalent amount as present in the extract, were applied intraperitoneally either 2 or 12 h prior to pyrogallol. As measured by the decrease in total score and tail moment, the DNA damage in liver was reduced by the extract and myricetin.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
T1  - Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract
SP  - 90
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6141
ER  - 

@conference{
author = "Matić, Sanja and Stanić, Snežana and Bogojević, Desanka and Solujić, Slavica and Mladenović, Milan and Stanković, Nevena and Mihailović, Vladimir and Katanić, Jelena and Mihailović, Mirjana",
year = "2013",
abstract = "The plant Cotinus coggygria Scop. (family Anacardiaceae) is commonly used in folk medicine for the treat­ment of various illnesses, such as diarrhea, paradontosis, gastric and duodenal ulcers. Different parts of this plant have been subjected to pharmacological evaluation for their potential antihaemorragic, wound-healing, anti­inflammatory, and antimicrobial activity. The present study was undertaken to investigate the phenolic conten according to the Folin-Ciocalteu procedure, while the spectrophotometric method with aluminium chloride was used for the determination of total ftavonoids. The phenolic and flavonoid composition of extract were deter­mined by the HPLC method. Antioxidant activiy was quantitatively determined using a DPPH radical scaveng­ing assay. To examine the antigenotoxic potential using the comet assay, Wistar rats were treated with 100 mg/ kg body weight of pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The content of total phenols and ftavonoids was 3.78 mg of gallic acid/g of dry plant material and 8.29 mg of rutin/g of dry plant material, respectively. HPLC analysis showed that myricetin was the dominant compound (511.5 µg/g), while hydroxyl derivatives of cinnammic acids (chlorogenic, caffeic, coumaric, ferulic and rosmaric acid) were identified in the extract in varying amount. The neutralisation of DPPH radicals was up to 95%, while the maximum inhibition concentration is approximately 125 µg/ml. The dose of 500 mg/kg body weight of the extract, that display no genotoxic activity, and its main flavonoid compound myricetin, in equivalent amount as present in the extract, were applied intraperitoneally either 2 or 12 h prior to pyrogallol. As measured by the decrease in total score and tail moment, the DNA damage in liver was reduced by the extract and myricetin.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia",
title = "Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract",
pages = "90-91",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6141"
}

Matić, S., Stanić, S., Bogojević, D., Solujić, S., Mladenović, M., Stanković, N., Mihailović, V., Katanić, J.,& Mihailović, M.. (2013). Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
Belgrade: Serbian Plant Physiology Society., 90-91.
https://hdl.handle.net/21.15107/rcub_ibiss_6141

Matić S, Stanić S, Bogojević D, Solujić S, Mladenović M, Stanković N, Mihailović V, Katanić J, Mihailović M. Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. 2013;:90-91.
https://hdl.handle.net/21.15107/rcub_ibiss_6141 .

Matić, Sanja, Stanić, Snežana, Bogojević, Desanka, Solujić, Slavica, Mladenović, Milan, Stanković, Nevena, Mihailović, Vladimir, Katanić, Jelena, Mihailović, Mirjana, "Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia (2013):90-91,
https://hdl.handle.net/21.15107/rcub_ibiss_6141 .

TY  - CONF
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Mihailović, Mirjana
AU  - Mišić, Danijela
AU  - Solujić, Slavica
AU  - Šipovac, Katarina
AU  - Stanković, Vesna
AU  - Mladenović, Milan
AU  - Stanković, Nevena
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6143
AB  - Gentiana plants are best known for their bitter taste that is due to the secoiridoids (e.g. swertiamarin, gen­tiopicrin, sweroside and amarogentin). Secoiridoid glucosides, gentiopicrin, swertiamarin and sweroside, are present in various traditional medicine preparations and are reported to have hepatoprotective activity. Many Gentiana species are known for their pharmaceutical values, such as Gentiana cruciata L., commonly called cross gentian. The dried roots and above-ground parts of G. cruciata are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing benefi­cial effects in gall and liver diseases. This study using in vivo model investigates hepatoprotective activity of the methanol extract of G. cruciata root (GCR) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCR (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days be­fore they were treated with CCl4 (1 ml/kg, 1 :1 mixture in olive oil) which caused liver injury. Pretreatment with GCR dose-dependently and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phos­phatase and total bilirubin, where as an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobar­bituric acid-reactive substances, as compared to CC14 treated group. Histological analyses also show that GCR reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macrove­sicular changes induced by CC14 in rats. The main secoiridoid compounds (sweroside, swertiamarin and gen­tiopicrin) present in GCR were identified and quantified to gain an insight into the compounds responsible for its hepatoprotective effects. The HPLC assay clearly indicated that GCR contained the greatest concentra­tion of gentiopicrin (5.45%), whereas concentration of sweroside (0.29%) and swertiamarin (0.09%) were lower.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
T1  - Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract
SP  - 91
EP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6143
ER  - 

@conference{
author = "Mihailović, Vladimir and Katanić, Jelena and Mihailović, Mirjana and Mišić, Danijela and Solujić, Slavica and Šipovac, Katarina and Stanković, Vesna and Mladenović, Milan and Stanković, Nevena",
year = "2013",
abstract = "Gentiana plants are best known for their bitter taste that is due to the secoiridoids (e.g. swertiamarin, gen­tiopicrin, sweroside and amarogentin). Secoiridoid glucosides, gentiopicrin, swertiamarin and sweroside, are present in various traditional medicine preparations and are reported to have hepatoprotective activity. Many Gentiana species are known for their pharmaceutical values, such as Gentiana cruciata L., commonly called cross gentian. The dried roots and above-ground parts of G. cruciata are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing benefi­cial effects in gall and liver diseases. This study using in vivo model investigates hepatoprotective activity of the methanol extract of G. cruciata root (GCR) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCR (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days be­fore they were treated with CCl4 (1 ml/kg, 1 :1 mixture in olive oil) which caused liver injury. Pretreatment with GCR dose-dependently and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phos­phatase and total bilirubin, where as an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobar­bituric acid-reactive substances, as compared to CC14 treated group. Histological analyses also show that GCR reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macrove­sicular changes induced by CC14 in rats. The main secoiridoid compounds (sweroside, swertiamarin and gen­tiopicrin) present in GCR were identified and quantified to gain an insight into the compounds responsible for its hepatoprotective effects. The HPLC assay clearly indicated that GCR contained the greatest concentra­tion of gentiopicrin (5.45%), whereas concentration of sweroside (0.29%) and swertiamarin (0.09%) were lower.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia",
title = "Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract",
pages = "91-92",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6143"
}

Mihailović, V., Katanić, J., Mihailović, M., Mišić, D., Solujić, S., Šipovac, K., Stanković, V., Mladenović, M.,& Stanković, N.. (2013). Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
Belgrade: Serbian Plant Physiology Society., 91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6143

Mihailović V, Katanić J, Mihailović M, Mišić D, Solujić S, Šipovac K, Stanković V, Mladenović M, Stanković N. Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. 2013;:91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6143 .

Mihailović, Vladimir, Katanić, Jelena, Mihailović, Mirjana, Mišić, Danijela, Solujić, Slavica, Šipovac, Katarina, Stanković, Vesna, Mladenović, Milan, Stanković, Nevena, "Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia (2013):91-92,
https://hdl.handle.net/21.15107/rcub_ibiss_6143 .