TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Renko, Kostja
AU  - Ajdžanović, Vladimir
AU  - Manojlović-Stojanoski, Milica
AU  - Milošević, Verica
AU  - Koehrle, Josef
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1091
AB  - Endogenous androgens are involved in regulation of thyroid function and metabolism of thyroid hormones. As serum testosterone level progressively declines with age, this regulation may change. We tested how androgen deprivation, achieved by orchidectomy, affects thyroid homeostasis in middle-aged rats. Fifteen-month-old Wistar rats were orchidectomized (Orx) or sham-operated under ketamine anesthesia (15 mg/kg body weight). Five weeks after the surgery, animals were decapitated. Thyroids were used for histomorphometric and ultrastructural examinations and together with livers and pituitaries for real-time quantitative PCR and deiodinase (DIO) activity measurements. Serum testosterone, TSH, L-thyroxine (T-4), and cholesterol (Chol) levels were determined. As expected, middle-aged control rats had lower (P<0.05) testosterone and T-4 compared with 3-month-old males. In the Orx middle-aged group, we detected diminished serum testosterone (P<0.05), no change in TSH and T-4 levels, and higher Chol level (P<0.05), in comparison with age-matched controls. Histomorphometric analysis of thyroid tissue revealed decreased relative volume densities of follicles and colloid (P<0.05). Relevant gene expressions and DIO1 enzyme activity were not changed in the thyroids of Orx rats. Liver Dio1 gene expression and DIO1 activity were decreased (P<0.05) in comparison with the control values. Pituitary levels of TSH beta, Dio1, and Dio2 mRNAs did not change, while DIO2 activity decreased (P<0.05). In conclusion, orchidectomy of middle-aged rats affected thyroid structure with no effect on serum T-4 and TSH. However, decreased liver DIO1 and pituitary DIO2 enzyme activities indicate compensatory-adaptive changes in local T-3 production. Journal of Endocrinology (2012) 215, 247-256
PB  - Society for Endocrinology
T2  - Journal of Endocrinology
T1  - Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity
IS  - 2
VL  - 215
DO  - 10.1530/JOE-12-0336
SP  - 325
EP  - 256
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1091
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Filipović, Branko and Renko, Kostja and Ajdžanović, Vladimir and Manojlović-Stojanoski, Milica and Milošević, Verica and Koehrle, Josef",
year = "2012",
abstract = "Endogenous androgens are involved in regulation of thyroid function and metabolism of thyroid hormones. As serum testosterone level progressively declines with age, this regulation may change. We tested how androgen deprivation, achieved by orchidectomy, affects thyroid homeostasis in middle-aged rats. Fifteen-month-old Wistar rats were orchidectomized (Orx) or sham-operated under ketamine anesthesia (15 mg/kg body weight). Five weeks after the surgery, animals were decapitated. Thyroids were used for histomorphometric and ultrastructural examinations and together with livers and pituitaries for real-time quantitative PCR and deiodinase (DIO) activity measurements. Serum testosterone, TSH, L-thyroxine (T-4), and cholesterol (Chol) levels were determined. As expected, middle-aged control rats had lower (P<0.05) testosterone and T-4 compared with 3-month-old males. In the Orx middle-aged group, we detected diminished serum testosterone (P<0.05), no change in TSH and T-4 levels, and higher Chol level (P<0.05), in comparison with age-matched controls. Histomorphometric analysis of thyroid tissue revealed decreased relative volume densities of follicles and colloid (P<0.05). Relevant gene expressions and DIO1 enzyme activity were not changed in the thyroids of Orx rats. Liver Dio1 gene expression and DIO1 activity were decreased (P<0.05) in comparison with the control values. Pituitary levels of TSH beta, Dio1, and Dio2 mRNAs did not change, while DIO2 activity decreased (P<0.05). In conclusion, orchidectomy of middle-aged rats affected thyroid structure with no effect on serum T-4 and TSH. However, decreased liver DIO1 and pituitary DIO2 enzyme activities indicate compensatory-adaptive changes in local T-3 production. Journal of Endocrinology (2012) 215, 247-256",
publisher = "Society for Endocrinology",
journal = "Journal of Endocrinology",
title = "Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity",
number = "2",
volume = "215",
doi = "10.1530/JOE-12-0336",
pages = "325-256",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1091"
}

Šošić-Jurjević, B., Filipović, B., Renko, K., Ajdžanović, V., Manojlović-Stojanoski, M., Milošević, V.,& Koehrle, J.. (2012). Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity. in Journal of Endocrinology
Society for Endocrinology., 215(2), 325-256.
https://doi.org/10.1530/JOE-12-0336
https://hdl.handle.net/21.15107/rcub_ibiss_1091

Šošić-Jurjević B, Filipović B, Renko K, Ajdžanović V, Manojlović-Stojanoski M, Milošević V, Koehrle J. Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity. in Journal of Endocrinology. 2012;215(2):325-256.
doi:10.1530/JOE-12-0336
https://hdl.handle.net/21.15107/rcub_ibiss_1091 .

Šošić-Jurjević, Branka , Filipović, Branko, Renko, Kostja, Ajdžanović, Vladimir, Manojlović-Stojanoski, Milica, Milošević, Verica, Koehrle, Josef, "Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity" in Journal of Endocrinology, 215, no. 2 (2012):325-256,
https://doi.org/10.1530/JOE-12-0336 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1091 .