TY  - JOUR
AU  - Čukuranović Kokoris, Jovana
AU  - Ajdžanović, Vladimir
AU  - Pendovski, Lazo
AU  - Ristić, Nataša
AU  - Milošević, Verica
AU  - Dovenska, Monika
AU  - Popovska-Perčinić, Florina
PY  - 2022
UR  - https://www.sciendo.com/article/10.2478/acve-2022-0001
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4965
AB  - Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.
T2  - Acta Veterinaria
T1  - The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study
IS  - 1
VL  - 72
DO  - 10.2478/acve-2022-0001
SP  - 1
EP  - 15
ER  - 

@article{
author = "Čukuranović Kokoris, Jovana and Ajdžanović, Vladimir and Pendovski, Lazo and Ristić, Nataša and Milošević, Verica and Dovenska, Monika and Popovska-Perčinić, Florina",
year = "2022",
abstract = "Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.",
journal = "Acta Veterinaria",
title = "The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study",
number = "1",
volume = "72",
doi = "10.2478/acve-2022-0001",
pages = "1-15"
}

Čukuranović Kokoris, J., Ajdžanović, V., Pendovski, L., Ristić, N., Milošević, V., Dovenska, M.,& Popovska-Perčinić, F.. (2022). The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study. in Acta Veterinaria, 72(1), 1-15.
https://doi.org/10.2478/acve-2022-0001

Čukuranović Kokoris J, Ajdžanović V, Pendovski L, Ristić N, Milošević V, Dovenska M, Popovska-Perčinić F. The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study. in Acta Veterinaria. 2022;72(1):1-15.
doi:10.2478/acve-2022-0001 .

Čukuranović Kokoris, Jovana, Ajdžanović, Vladimir, Pendovski, Lazo, Ristić, Nataša, Milošević, Verica, Dovenska, Monika, Popovska-Perčinić, Florina, "The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study" in Acta Veterinaria, 72, no. 1 (2022):1-15,
https://doi.org/10.2478/acve-2022-0001 . .

TY  - JOUR
AU  - Milošević, Verica
AU  - Severs, Walter B
AU  - Ristić, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Popovska-Perčinić, Florina V
AU  - Šošić-Jurjević, Branka 
AU  - Pendovski, Lazo B
AU  - Trifunović, Svetlana
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
PY  - 2018
UR  - http://www.hh.um.es/Abstracts/Vol_/_/__11984.htm
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3103
AB  - Genistein (G) and related soy phytoestrogens have been studied for potential usefulness in different chronic diseases, and may ameliorate signs of aging. They have a profound influence on the hypothalamo-pituitary-adrenal (HPA) axis. The present study utilized the rat model of mild andropause to thoroughly evaluate the effects of G and soy extract on the adrenal gland and related blood hormones. Adult male rats were orchidectomized (Orx) or sham operated (SO). Orx rats received daily subcutaneous injections for 3 weeks of solvent, or G (Orx+G, 30 mg/kg), or commercial soy extract (Orx+Soy, 30 mg/kg). Adrenal glands and blood were harvested at the end of the treatment for hormone analyses, histology and design-based stereology. Compared to SO rats Orx evoked significant (P<0.05) changes including: the replicating cell number in the 3 adrenocortical zones; vascularity and cortical volume and blood levels of adrenocorticotropic hormone (ACTH), aldosterone and dehydroepiandrosterone (DHEA). When comparing Orx vs. Orx+G groups the following significant (P<0.05) changes were observed: a further increase in number of replicating cells in zonas glomerulosa and reticularis, vasculature network presence, cortical and zona reticularis volumes, ACTH and corticosterone concentrations, and lower DHEA levels. Comparing Orx vs. Orx+Soy resulted in elevated (P<0.05) ACTH and corticosterone levels. Structural integrity of the adrenal gland was unchanged vs. SO rats. Overall, G and soy extract treatments resulted in proliferative activity and/or vasculature support in the adrenal cortex. The data and current literature support the impression of a beneficial effect of soy components on the homeostatic response to stress.
T2  - Histology and Histopathology
T1  - Soy isoflavone effects on the adrenal glands of orchidectomized adult male rats: a comprehensive histological and hormonal study.
IS  - 8
VL  - 33
DO  - 10.14670/HH-11-984
SP  - 843
EP  - 857
ER  - 

@article{
author = "Milošević, Verica and Severs, Walter B and Ristić, Nataša and Manojlović-Stojanoski, Milica and Popovska-Perčinić, Florina V and Šošić-Jurjević, Branka  and Pendovski, Lazo B and Trifunović, Svetlana and Miler, Marko and Ajdžanović, Vladimir",
year = "2018",
abstract = "Genistein (G) and related soy phytoestrogens have been studied for potential usefulness in different chronic diseases, and may ameliorate signs of aging. They have a profound influence on the hypothalamo-pituitary-adrenal (HPA) axis. The present study utilized the rat model of mild andropause to thoroughly evaluate the effects of G and soy extract on the adrenal gland and related blood hormones. Adult male rats were orchidectomized (Orx) or sham operated (SO). Orx rats received daily subcutaneous injections for 3 weeks of solvent, or G (Orx+G, 30 mg/kg), or commercial soy extract (Orx+Soy, 30 mg/kg). Adrenal glands and blood were harvested at the end of the treatment for hormone analyses, histology and design-based stereology. Compared to SO rats Orx evoked significant (P<0.05) changes including: the replicating cell number in the 3 adrenocortical zones; vascularity and cortical volume and blood levels of adrenocorticotropic hormone (ACTH), aldosterone and dehydroepiandrosterone (DHEA). When comparing Orx vs. Orx+G groups the following significant (P<0.05) changes were observed: a further increase in number of replicating cells in zonas glomerulosa and reticularis, vasculature network presence, cortical and zona reticularis volumes, ACTH and corticosterone concentrations, and lower DHEA levels. Comparing Orx vs. Orx+Soy resulted in elevated (P<0.05) ACTH and corticosterone levels. Structural integrity of the adrenal gland was unchanged vs. SO rats. Overall, G and soy extract treatments resulted in proliferative activity and/or vasculature support in the adrenal cortex. The data and current literature support the impression of a beneficial effect of soy components on the homeostatic response to stress.",
journal = "Histology and Histopathology",
title = "Soy isoflavone effects on the adrenal glands of orchidectomized adult male rats: a comprehensive histological and hormonal study.",
number = "8",
volume = "33",
doi = "10.14670/HH-11-984",
pages = "843-857"
}

Milošević, V., Severs, W. B., Ristić, N., Manojlović-Stojanoski, M., Popovska-Perčinić, F. V., Šošić-Jurjević, B., Pendovski, L. B., Trifunović, S., Miler, M.,& Ajdžanović, V.. (2018). Soy isoflavone effects on the adrenal glands of orchidectomized adult male rats: a comprehensive histological and hormonal study.. in Histology and Histopathology, 33(8), 843-857.
https://doi.org/10.14670/HH-11-984

Milošević V, Severs WB, Ristić N, Manojlović-Stojanoski M, Popovska-Perčinić FV, Šošić-Jurjević B, Pendovski LB, Trifunović S, Miler M, Ajdžanović V. Soy isoflavone effects on the adrenal glands of orchidectomized adult male rats: a comprehensive histological and hormonal study.. in Histology and Histopathology. 2018;33(8):843-857.
doi:10.14670/HH-11-984 .

Milošević, Verica, Severs, Walter B, Ristić, Nataša, Manojlović-Stojanoski, Milica, Popovska-Perčinić, Florina V, Šošić-Jurjević, Branka , Pendovski, Lazo B, Trifunović, Svetlana, Miler, Marko, Ajdžanović, Vladimir, "Soy isoflavone effects on the adrenal glands of orchidectomized adult male rats: a comprehensive histological and hormonal study." in Histology and Histopathology, 33, no. 8 (2018):843-857,
https://doi.org/10.14670/HH-11-984 . .

TY  - JOUR
AU  - Popovska-Perčinić, Florina
AU  - Ajdžanović, Vladimir
AU  - Pendovski, Lazo
AU  - Živanović, Jasmina
AU  - Ilieski, Vlatko
AU  - Dovenska, Monika
AU  - Milošević, Verica
PY  - 2012
UR  - https://macvetrev.mk/LoadAbstract?DOI=591.481.2.044.599.323.4.084
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6898
AB  - Growth hormone (GH) axis function appears to be changed in the warm milieu. The effect of chronic exposure to moderate heat on immuno-histomorphometric and –fluorescent characteristics of pituitary GH cells, in adult male rats, was examined. The experimental group was exposed to 35±1oC for 30 days, whereas the control group was kept at room temperature during the same period. GH cells were studied using the adequate immunostaining procedures. The body weight of animals in the experimental group was significantly decreased by 24.5% compared to the controls. Immuno-histochemically and –fluorescently identified GH cells in controls were intensely stained, oval in shape, with the centrally located spherical nucleus. In rats exposed to moderate heat the localization of GH cells was not significantly changed, while their shape was slightly different. They were mostly organized in groups, with darker cytoplasmic regions/higher intensity of immunofluorescence signal throughout the whole cytoplasm. The cellular and nuclear volumes of GH cells in the experimental group were significantly decreased by 16.0% and 9.0% respectively, but the volume density was only slightly decreased in comparison with the controls. These findings suggest that 30 days of continuous exposure of adult male rats to moderately high ambient temperature has an inhibitory effect on the immuno-histomorphometric characteristics and increases the immuno-fluorescence signal of GH cells.
PB  - Skopje: Faculty of Veterinary Medicine, Ss. Cyril and Methodius University in Skopje
T2  - Macedonian Veterinary Review
T1  - Immuno-histomorphometric and -fluorescent characteristics of rat GH cells after chronic exposure to moderate heat
IS  - 2
VL  - 35
SP  - 65
EP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6898
ER  - 

@article{
author = "Popovska-Perčinić, Florina and Ajdžanović, Vladimir and Pendovski, Lazo and Živanović, Jasmina and Ilieski, Vlatko and Dovenska, Monika and Milošević, Verica",
year = "2012",
abstract = "Growth hormone (GH) axis function appears to be changed in the warm milieu. The effect of chronic exposure to moderate heat on immuno-histomorphometric and –fluorescent characteristics of pituitary GH cells, in adult male rats, was examined. The experimental group was exposed to 35±1oC for 30 days, whereas the control group was kept at room temperature during the same period. GH cells were studied using the adequate immunostaining procedures. The body weight of animals in the experimental group was significantly decreased by 24.5% compared to the controls. Immuno-histochemically and –fluorescently identified GH cells in controls were intensely stained, oval in shape, with the centrally located spherical nucleus. In rats exposed to moderate heat the localization of GH cells was not significantly changed, while their shape was slightly different. They were mostly organized in groups, with darker cytoplasmic regions/higher intensity of immunofluorescence signal throughout the whole cytoplasm. The cellular and nuclear volumes of GH cells in the experimental group were significantly decreased by 16.0% and 9.0% respectively, but the volume density was only slightly decreased in comparison with the controls. These findings suggest that 30 days of continuous exposure of adult male rats to moderately high ambient temperature has an inhibitory effect on the immuno-histomorphometric characteristics and increases the immuno-fluorescence signal of GH cells.",
publisher = "Skopje: Faculty of Veterinary Medicine, Ss. Cyril and Methodius University in Skopje",
journal = "Macedonian Veterinary Review",
title = "Immuno-histomorphometric and -fluorescent characteristics of rat GH cells after chronic exposure to moderate heat",
number = "2",
volume = "35",
pages = "65-72",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6898"
}

Popovska-Perčinić, F., Ajdžanović, V., Pendovski, L., Živanović, J., Ilieski, V., Dovenska, M.,& Milošević, V.. (2012). Immuno-histomorphometric and -fluorescent characteristics of rat GH cells after chronic exposure to moderate heat. in Macedonian Veterinary Review
Skopje: Faculty of Veterinary Medicine, Ss. Cyril and Methodius University in Skopje., 35(2), 65-72.
https://hdl.handle.net/21.15107/rcub_ibiss_6898

Popovska-Perčinić F, Ajdžanović V, Pendovski L, Živanović J, Ilieski V, Dovenska M, Milošević V. Immuno-histomorphometric and -fluorescent characteristics of rat GH cells after chronic exposure to moderate heat. in Macedonian Veterinary Review. 2012;35(2):65-72.
https://hdl.handle.net/21.15107/rcub_ibiss_6898 .

Popovska-Perčinić, Florina, Ajdžanović, Vladimir, Pendovski, Lazo, Živanović, Jasmina, Ilieski, Vlatko, Dovenska, Monika, Milošević, Verica, "Immuno-histomorphometric and -fluorescent characteristics of rat GH cells after chronic exposure to moderate heat" in Macedonian Veterinary Review, 35, no. 2 (2012):65-72,
https://hdl.handle.net/21.15107/rcub_ibiss_6898 .

TY  - CONF
AU  - Perčinić-Popovska, Florina
AU  - Ajdžanović, Vladimir
AU  - Trifunović, Svetlana
AU  - Jordanova, Maja
AU  - Ilieski, Vlatko
AU  - Pendovski, Lazo
AU  - Milošević, Verica
PY  - 2011
UR  - https://www.endocrine-abstracts.org/ea/0026/ea0026p1
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6909
AB  - The global warming and its impact on the summer average temperature elevation, in the south-eastern parts of Europe, represent an inevitable stress for living organisms in this region. Heat stress was shown to be the strongest one, when compared to immobilization or cold stress. Activation of the hypothalamo-pituitary-adrenal (HPA) axis during exposure to heat stress on the morphology of pituitary adrenocorticotropes (ACTH cells) and adrenal cortex, especially on zona fasciculata (ZF), in adult male Wistar rats, was examined. The experimental animals (n=7) were exposed for 24h to high ambient temperature (35±1°C). The control group (n=7) was kept at room temperature (20±2°C). The pituitary and left adrenal glands were fixed in Bouin’s solution and serially cut (5 μm). The ACTH cells were identified by the peroxidase-antiperoxidase (PAP) immunohistochemical procedure, while the cells of adrenal cortex were stained with hematoxylin-eosin. The volume of ACTH- and ZF- cells and their nuclei were estimated using the multipurpose test system M42. The body weight in heat stressed rats was decreased (P<0.05) by 15.3%, compared to the controls. The relative pituitary weight was significantly increased (P<0.05) by 16.5%, in comparison with the corresponding control. The ACTH cell volume, as well as the volume density, were significantly decreased (P<0.05) by 12.3 and 26.7% respectively, in comparison with the controls. Absolute volumes of the adrenal gland, adrenal cortex, ZF, as well as the ZF cells were also decreased (P<0.05) by 10.7, 8.6, 15.2 and 3.3% respectively, compared to the controls. These findings suggest that one day exposure of adult male rats to high ambient temperature (35±1°C) has the inhibitory effect on the morphological characteristics of ACTH- and ZF-cells in adult male rats.
PB  - BioScientifica
C3  - 13th European Congress of Endocrinology; 2011 Apr 30 - May 4; Rotterda, The Netherlands
T1  - Pituitary–adrenal axis in male rats after exposure to high ambient temperature
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6909
ER  - 

@conference{
author = "Perčinić-Popovska, Florina and Ajdžanović, Vladimir and Trifunović, Svetlana and Jordanova, Maja and Ilieski, Vlatko and Pendovski, Lazo and Milošević, Verica",
year = "2011",
abstract = "The global warming and its impact on the summer average temperature elevation, in the south-eastern parts of Europe, represent an inevitable stress for living organisms in this region. Heat stress was shown to be the strongest one, when compared to immobilization or cold stress. Activation of the hypothalamo-pituitary-adrenal (HPA) axis during exposure to heat stress on the morphology of pituitary adrenocorticotropes (ACTH cells) and adrenal cortex, especially on zona fasciculata (ZF), in adult male Wistar rats, was examined. The experimental animals (n=7) were exposed for 24h to high ambient temperature (35±1°C). The control group (n=7) was kept at room temperature (20±2°C). The pituitary and left adrenal glands were fixed in Bouin’s solution and serially cut (5 μm). The ACTH cells were identified by the peroxidase-antiperoxidase (PAP) immunohistochemical procedure, while the cells of adrenal cortex were stained with hematoxylin-eosin. The volume of ACTH- and ZF- cells and their nuclei were estimated using the multipurpose test system M42. The body weight in heat stressed rats was decreased (P<0.05) by 15.3%, compared to the controls. The relative pituitary weight was significantly increased (P<0.05) by 16.5%, in comparison with the corresponding control. The ACTH cell volume, as well as the volume density, were significantly decreased (P<0.05) by 12.3 and 26.7% respectively, in comparison with the controls. Absolute volumes of the adrenal gland, adrenal cortex, ZF, as well as the ZF cells were also decreased (P<0.05) by 10.7, 8.6, 15.2 and 3.3% respectively, compared to the controls. These findings suggest that one day exposure of adult male rats to high ambient temperature (35±1°C) has the inhibitory effect on the morphological characteristics of ACTH- and ZF-cells in adult male rats.",
publisher = "BioScientifica",
journal = "13th European Congress of Endocrinology; 2011 Apr 30 - May 4; Rotterda, The Netherlands",
title = "Pituitary–adrenal axis in male rats after exposure to high ambient temperature",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6909"
}

Perčinić-Popovska, F., Ajdžanović, V., Trifunović, S., Jordanova, M., Ilieski, V., Pendovski, L.,& Milošević, V.. (2011). Pituitary–adrenal axis in male rats after exposure to high ambient temperature. in 13th European Congress of Endocrinology; 2011 Apr 30 - May 4; Rotterda, The Netherlands
BioScientifica..
https://hdl.handle.net/21.15107/rcub_ibiss_6909

Perčinić-Popovska F, Ajdžanović V, Trifunović S, Jordanova M, Ilieski V, Pendovski L, Milošević V. Pituitary–adrenal axis in male rats after exposure to high ambient temperature. in 13th European Congress of Endocrinology; 2011 Apr 30 - May 4; Rotterda, The Netherlands. 2011;.
https://hdl.handle.net/21.15107/rcub_ibiss_6909 .

Perčinić-Popovska, Florina, Ajdžanović, Vladimir, Trifunović, Svetlana, Jordanova, Maja, Ilieski, Vlatko, Pendovski, Lazo, Milošević, Verica, "Pituitary–adrenal axis in male rats after exposure to high ambient temperature" in 13th European Congress of Endocrinology; 2011 Apr 30 - May 4; Rotterda, The Netherlands (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_6909 .