TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka
AU  - Živanović, Jasmina
AU  - Miler, Marko
AU  - Stanković, Sanja
AU  - Ranin, Jovan
AU  - Filipović, Branko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6999
AB  - Introduction and aim.
Vitamin D supplementation in aging subjects manifests the positive effect on various health-related parameters. We performed functionally-histological analysis of the adrenal cortex regarding the factors of vitamin D activity and corticosterone output, after vitamin D3 application in a rat model of the andropause.
Material and methods.
Middle-aged Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and vitamin D3 treated orchidectomized (Orx+vit. D; n=8) groups. Vitamin D3 (5 μg/kg b.m.) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using histochemistry/immunohistochemistry, stereology, ultrastructural and biochemical analyses.
Results.
Orchidectomy (Orx) decreased the adrenal cortex-related volume densities of vascular (p<0,0001), vitamin D receptor (VDR; p<0,0166), cytochrome P450 oxidase 2R1 (CYP 2R1; p<0,0001) and cytochrome P450 oxidase 24 (CYP 24; p<0,0001) depots, but increased the volume density of cytochrome P450 27B1 (CYP 27B1; p<0,0001) depots. In Orx+vit. D rats, increase of the adrenal cortex-related volume densities of collagen (p<0,0001), VDR (p<0,0001) and CYP 2R1 (p<0,0001) depots as well as the lipid-droplet diameter (p<0,0001) in adrenocortical outer zona fasciculata cells was observed, while a decrease of volume densities of the vascular (p<0,0001), CYP 27B1 (p<0,0001) and CYP 24 (p<0,0001) depots was registered, all versus Orx group. Plasma level of ACTH was decreased (p=0,0155) and serum concentrations of 25-hydroxyvitamin D3 and corticosterone were increased (p<0,0001 and p=0,0187, respectively), all after the same treatment.
Conclusions.
Increased corticosterone output after vitamin D3 application to andropausal rats appears not to be related to increased availability of 25-hydroxyvitamin D3 and decreased degradation of 1,25-dihydroxyvitamin D3 in adrenal tissue, but rather involves the central regulatory mechanisms.
PB  - Elsevier GmbH.
T2  - Annals of Anatomy
T1  - Vitamin D3 application and factors of its activity in the adrenal cortex of andropausal rats: A functionally-histological study
IS  - 2024
VL  - 256
DO  - 10.1016/j.aanat.2024.152322
SP  - 152322
ER  - 

@article{
author = "Ajdžanović, Vladimir and Šošić-Jurjević, Branka and Živanović, Jasmina and Miler, Marko and Stanković, Sanja and Ranin, Jovan and Filipović, Branko",
year = "2024",
abstract = "Introduction and aim.
Vitamin D supplementation in aging subjects manifests the positive effect on various health-related parameters. We performed functionally-histological analysis of the adrenal cortex regarding the factors of vitamin D activity and corticosterone output, after vitamin D3 application in a rat model of the andropause.
Material and methods.
Middle-aged Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and vitamin D3 treated orchidectomized (Orx+vit. D; n=8) groups. Vitamin D3 (5 μg/kg b.m.) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using histochemistry/immunohistochemistry, stereology, ultrastructural and biochemical analyses.
Results.
Orchidectomy (Orx) decreased the adrenal cortex-related volume densities of vascular (p<0,0001), vitamin D receptor (VDR; p<0,0166), cytochrome P450 oxidase 2R1 (CYP 2R1; p<0,0001) and cytochrome P450 oxidase 24 (CYP 24; p<0,0001) depots, but increased the volume density of cytochrome P450 27B1 (CYP 27B1; p<0,0001) depots. In Orx+vit. D rats, increase of the adrenal cortex-related volume densities of collagen (p<0,0001), VDR (p<0,0001) and CYP 2R1 (p<0,0001) depots as well as the lipid-droplet diameter (p<0,0001) in adrenocortical outer zona fasciculata cells was observed, while a decrease of volume densities of the vascular (p<0,0001), CYP 27B1 (p<0,0001) and CYP 24 (p<0,0001) depots was registered, all versus Orx group. Plasma level of ACTH was decreased (p=0,0155) and serum concentrations of 25-hydroxyvitamin D3 and corticosterone were increased (p<0,0001 and p=0,0187, respectively), all after the same treatment.
Conclusions.
Increased corticosterone output after vitamin D3 application to andropausal rats appears not to be related to increased availability of 25-hydroxyvitamin D3 and decreased degradation of 1,25-dihydroxyvitamin D3 in adrenal tissue, but rather involves the central regulatory mechanisms.",
publisher = "Elsevier GmbH.",
journal = "Annals of Anatomy",
title = "Vitamin D3 application and factors of its activity in the adrenal cortex of andropausal rats: A functionally-histological study",
number = "2024",
volume = "256",
doi = "10.1016/j.aanat.2024.152322",
pages = "152322"
}

Ajdžanović, V., Šošić-Jurjević, B., Živanović, J., Miler, M., Stanković, S., Ranin, J.,& Filipović, B.. (2024). Vitamin D3 application and factors of its activity in the adrenal cortex of andropausal rats: A functionally-histological study. in Annals of Anatomy
Elsevier GmbH.., 256(2024), 152322.
https://doi.org/10.1016/j.aanat.2024.152322

Ajdžanović V, Šošić-Jurjević B, Živanović J, Miler M, Stanković S, Ranin J, Filipović B. Vitamin D3 application and factors of its activity in the adrenal cortex of andropausal rats: A functionally-histological study. in Annals of Anatomy. 2024;256(2024):152322.
doi:10.1016/j.aanat.2024.152322 .

Ajdžanović, Vladimir, Šošić-Jurjević, Branka, Živanović, Jasmina, Miler, Marko, Stanković, Sanja, Ranin, Jovan, Filipović, Branko, "Vitamin D3 application and factors of its activity in the adrenal cortex of andropausal rats: A functionally-histological study" in Annals of Anatomy, 256, no. 2024 (2024):152322,
https://doi.org/10.1016/j.aanat.2024.152322 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka
AU  - Živanović, Jasmina
AU  - Miler, Marko
AU  - Stanković, Sanja
AU  - Ranin, Jovan
AU  - Filipović, Branko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/7000
AB  - Introduction and aim.Vitamin D supplementation in aging subjects manifests the positive effect on various health-related parameters. We performed functionally-histological analysis of the adrenal cortex regarding the factors of vitamin D activity and corticosterone output, after vitamin D3 application in a rat model of the andropause.Material and methods.Middle-aged Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and vitamin D3 treated orchidectomized (Orx+vit. D; n=8) groups. Vitamin D3 (5 μg/kg b.m.) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using histochemistry/immunohistochemistry, stereology, ultrastructural and biochemical analyses.Results.Orchidectomy (Orx) decreased the adrenal cortex-related volume densities of vascular (p<0,0001), vitamin D receptor (VDR; p<0,0166), cytochrome P450 oxidase 2R1 (CYP 2R1; p<0,0001) and cytochrome P450 oxidase 24 (CYP 24; p<0,0001) depots, but increased the volume density of cytochrome P450 27B1 (CYP 27B1; p<0,0001) depots. In Orx+vit. D rats, increase of the adrenal cortex-related volume densities of collagen (p<0,0001), VDR (p<0,0001) and CYP 2R1 (p<0,0001) depots as well as the lipid-droplet diameter (p<0,0001) in adrenocortical outer zona fasciculata cells was observed, while a decrease of volume densities of the vascular (p<0,0001), CYP 27B1 (p<0,0001) and CYP 24 (p<0,0001) depots was registered, all versus Orx group. Plasma level of ACTH was decreased (p=0,0155) and serum concentrations of 25-hydroxyvitamin D3 and corticosterone were increased (p<0,0001 and p=0,0187, respectively), all after the same treatment.Conclusions.Increased corticosterone output after vitamin D3 application to andropausal rats appears not to be related to increased availability of 25-hydroxyvitamin D3 and decreased degradation of 1,25-dihydroxyvitamin D3 in adrenal tissue, but rather involves the central regulatory mechanisms.
PB  - Elsevier GmbH.
T2  - Annals of Anatomy
T1  - Vitamin D3 application and factors of its activity in the adrenal cortex of andropausal rats: A functionally-histological study
IS  - 2024
VL  - 256
DO  - 10.1016/j.aanat.2024.152322
SP  - 152322
ER  - 

@article{
author = "Ajdžanović, Vladimir and Šošić-Jurjević, Branka and Živanović, Jasmina and Miler, Marko and Stanković, Sanja and Ranin, Jovan and Filipović, Branko",
year = "2024",
abstract = "Introduction and aim.Vitamin D supplementation in aging subjects manifests the positive effect on various health-related parameters. We performed functionally-histological analysis of the adrenal cortex regarding the factors of vitamin D activity and corticosterone output, after vitamin D3 application in a rat model of the andropause.Material and methods.Middle-aged Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and vitamin D3 treated orchidectomized (Orx+vit. D; n=8) groups. Vitamin D3 (5 μg/kg b.m.) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using histochemistry/immunohistochemistry, stereology, ultrastructural and biochemical analyses.Results.Orchidectomy (Orx) decreased the adrenal cortex-related volume densities of vascular (p<0,0001), vitamin D receptor (VDR; p<0,0166), cytochrome P450 oxidase 2R1 (CYP 2R1; p<0,0001) and cytochrome P450 oxidase 24 (CYP 24; p<0,0001) depots, but increased the volume density of cytochrome P450 27B1 (CYP 27B1; p<0,0001) depots. In Orx+vit. D rats, increase of the adrenal cortex-related volume densities of collagen (p<0,0001), VDR (p<0,0001) and CYP 2R1 (p<0,0001) depots as well as the lipid-droplet diameter (p<0,0001) in adrenocortical outer zona fasciculata cells was observed, while a decrease of volume densities of the vascular (p<0,0001), CYP 27B1 (p<0,0001) and CYP 24 (p<0,0001) depots was registered, all versus Orx group. Plasma level of ACTH was decreased (p=0,0155) and serum concentrations of 25-hydroxyvitamin D3 and corticosterone were increased (p<0,0001 and p=0,0187, respectively), all after the same treatment.Conclusions.Increased corticosterone output after vitamin D3 application to andropausal rats appears not to be related to increased availability of 25-hydroxyvitamin D3 and decreased degradation of 1,25-dihydroxyvitamin D3 in adrenal tissue, but rather involves the central regulatory mechanisms.",
publisher = "Elsevier GmbH.",
journal = "Annals of Anatomy",
title = "Vitamin D3 application and factors of its activity in the adrenal cortex of andropausal rats: A functionally-histological study",
number = "2024",
volume = "256",
doi = "10.1016/j.aanat.2024.152322",
pages = "152322"
}

Ajdžanović, V., Šošić-Jurjević, B., Živanović, J., Miler, M., Stanković, S., Ranin, J.,& Filipović, B.. (2024). Vitamin D3 application and factors of its activity in the adrenal cortex of andropausal rats: A functionally-histological study. in Annals of Anatomy
Elsevier GmbH.., 256(2024), 152322.
https://doi.org/10.1016/j.aanat.2024.152322

Ajdžanović V, Šošić-Jurjević B, Živanović J, Miler M, Stanković S, Ranin J, Filipović B. Vitamin D3 application and factors of its activity in the adrenal cortex of andropausal rats: A functionally-histological study. in Annals of Anatomy. 2024;256(2024):152322.
doi:10.1016/j.aanat.2024.152322 .

Ajdžanović, Vladimir, Šošić-Jurjević, Branka, Živanović, Jasmina, Miler, Marko, Stanković, Sanja, Ranin, Jovan, Filipović, Branko, "Vitamin D3 application and factors of its activity in the adrenal cortex of andropausal rats: A functionally-histological study" in Annals of Anatomy, 256, no. 2024 (2024):152322,
https://doi.org/10.1016/j.aanat.2024.152322 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka
AU  - Ranin, Jovan
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6078
AB  - In his Theory of relativity, Einstein determined that the time is relative to the reference frame of the observer. Under specific conditions, there is a difference in the elapsed time between two clocks, known as time dilation. A similar relativistic effect could be attributed to the brain operating at different frequencies, e.g. while it is slow and during the thought process. Time flow and the aging process are causally linked. Herein, we introduce physical relativity into the mind/thought context and elaborate changed perception of the time flow (the impression of the time acceleration) with aging. The phenomenology of time is observed in the context of physical and biological clock, as well as by introducing the category of ‘mind time’. Mental processing impairment is crucial for the “aging-caused relativity of time”, while adjusting of its’ perception seems to be a matter of body/mind rest, mental hygiene and physical activity of the aging subject. We also provide a brief overview of the perception of time flow in some disease states that coincide with aging. Our main idea has a perspective for future development in the interdisciplinary synergy of philosophy, physical-mathematical elaboration, experimental biology and clinical investigations.
PB  - Heidelberg: Springer Nature
T2  - Biologia Futura
T1  - Biologia Futura: does the aging process contribute to the relativity of time?
IS  - 74
IS  - 1-2
DO  - 10.1007/s42977-023-00167-2
SP  - 137
EP  - 143
ER  - 

@article{
author = "Ajdžanović, Vladimir and Šošić-Jurjević, Branka and Ranin, Jovan and Filipović, Branko",
year = "2023",
abstract = "In his Theory of relativity, Einstein determined that the time is relative to the reference frame of the observer. Under specific conditions, there is a difference in the elapsed time between two clocks, known as time dilation. A similar relativistic effect could be attributed to the brain operating at different frequencies, e.g. while it is slow and during the thought process. Time flow and the aging process are causally linked. Herein, we introduce physical relativity into the mind/thought context and elaborate changed perception of the time flow (the impression of the time acceleration) with aging. The phenomenology of time is observed in the context of physical and biological clock, as well as by introducing the category of ‘mind time’. Mental processing impairment is crucial for the “aging-caused relativity of time”, while adjusting of its’ perception seems to be a matter of body/mind rest, mental hygiene and physical activity of the aging subject. We also provide a brief overview of the perception of time flow in some disease states that coincide with aging. Our main idea has a perspective for future development in the interdisciplinary synergy of philosophy, physical-mathematical elaboration, experimental biology and clinical investigations.",
publisher = "Heidelberg: Springer Nature",
journal = "Biologia Futura",
title = "Biologia Futura: does the aging process contribute to the relativity of time?",
number = "74, 1-2",
doi = "10.1007/s42977-023-00167-2",
pages = "137-143"
}

Ajdžanović, V., Šošić-Jurjević, B., Ranin, J.,& Filipović, B.. (2023). Biologia Futura: does the aging process contribute to the relativity of time?. in Biologia Futura
Heidelberg: Springer Nature.(74), 137-143.
https://doi.org/10.1007/s42977-023-00167-2

Ajdžanović V, Šošić-Jurjević B, Ranin J, Filipović B. Biologia Futura: does the aging process contribute to the relativity of time?. in Biologia Futura. 2023;(74):137-143.
doi:10.1007/s42977-023-00167-2 .

Ajdžanović, Vladimir, Šošić-Jurjević, Branka, Ranin, Jovan, Filipović, Branko, "Biologia Futura: does the aging process contribute to the relativity of time?" in Biologia Futura, no. 74 (2023):137-143,
https://doi.org/10.1007/s42977-023-00167-2 . .