TY  - CONF
AU  - Đorđević, Jelena
AU  - Oalđe Pavlović, Mariana
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Duletić-Laušević, Sonja
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5729
AB  - BACKGROUND
According to World Health Organization, 70-95% of the population chooses folk medicine as their primary
approach for health maintenance. In addition to their healing properties and application in folk medicine,
Lamiaceae plants are often valued in cookery as spices and food preservatives.
OBJECTIVES
Hence, the aim of this study was to examine the antibiofilm activity of 12 ethanolic extracts of Lamiaceae species
on biofilm formation and the degradation of existing biofilm of Pseudomonas aeruginosa PAO1 (ATCC 15692).
METHODS
The crystal violet staining method was used to evaluate the newly formed bacterial biofilms. Four concentrations
(double dilutions) of plant extracts (starting conc. 2500 μg/mL), solvent control (ethanol, starting conc.15%),
and positive control (streptomycin, starting conc. 12.5 μg/mL) were tested.082
RESULTS
All ethanol extracts showed antibiofilm activity. However, the strongest activity was observed for Hyssopus
officinalis, Melissa officinalis, Mentha piperita, and Ocimum basilicum, where the viability of bacteria in the
biofilm after treatment was about 60% mainly at each tested concentration. Teucrium chamaedrys extract
exhibited the strongest activity by degrading about 60% of biofilm (about 40% viability) at all concentrations
except at the lowest tested one. Promising results were also observed for M. piperita (at each of the tested
concentrations) and O. basilicum (at the highest tested concentration), where the viability of bacteria in the
biofilm after treatment was reduced by about 40%. The remaining extracts showed a slightly lower effect on
the degradation of the previously formed biofilm of P. aeruginosa PAO1.
PB  - Belgrade: Serbian Society of Microbiology
C3  - Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
T1  - Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?
SP  - 801
EP  - 802
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5729
ER  - 

@conference{
author = "Đorđević, Jelena and Oalđe Pavlović, Mariana and Jovanović Marić, Jovana and Kolarević, Stoimir and Duletić-Laušević, Sonja and Vuković-Gačić, Branka",
year = "2022",
abstract = "BACKGROUND
According to World Health Organization, 70-95% of the population chooses folk medicine as their primary
approach for health maintenance. In addition to their healing properties and application in folk medicine,
Lamiaceae plants are often valued in cookery as spices and food preservatives.
OBJECTIVES
Hence, the aim of this study was to examine the antibiofilm activity of 12 ethanolic extracts of Lamiaceae species
on biofilm formation and the degradation of existing biofilm of Pseudomonas aeruginosa PAO1 (ATCC 15692).
METHODS
The crystal violet staining method was used to evaluate the newly formed bacterial biofilms. Four concentrations
(double dilutions) of plant extracts (starting conc. 2500 μg/mL), solvent control (ethanol, starting conc.15%),
and positive control (streptomycin, starting conc. 12.5 μg/mL) were tested.082
RESULTS
All ethanol extracts showed antibiofilm activity. However, the strongest activity was observed for Hyssopus
officinalis, Melissa officinalis, Mentha piperita, and Ocimum basilicum, where the viability of bacteria in the
biofilm after treatment was about 60% mainly at each tested concentration. Teucrium chamaedrys extract
exhibited the strongest activity by degrading about 60% of biofilm (about 40% viability) at all concentrations
except at the lowest tested one. Promising results were also observed for M. piperita (at each of the tested
concentrations) and O. basilicum (at the highest tested concentration), where the viability of bacteria in the
biofilm after treatment was reduced by about 40%. The remaining extracts showed a slightly lower effect on
the degradation of the previously formed biofilm of P. aeruginosa PAO1.",
publisher = "Belgrade: Serbian Society of Microbiology",
journal = "Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia",
title = "Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?",
pages = "801-802",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5729"
}

Đorđević, J., Oalđe Pavlović, M., Jovanović Marić, J., Kolarević, S., Duletić-Laušević, S.,& Vuković-Gačić, B.. (2022). Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia
Belgrade: Serbian Society of Microbiology., 801-802.
https://hdl.handle.net/21.15107/rcub_ibiss_5729

Đorđević J, Oalđe Pavlović M, Jovanović Marić J, Kolarević S, Duletić-Laušević S, Vuković-Gačić B. Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?. in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia. 2022;:801-802.
https://hdl.handle.net/21.15107/rcub_ibiss_5729 .

Đorđević, Jelena, Oalđe Pavlović, Mariana, Jovanović Marić, Jovana, Kolarević, Stoimir, Duletić-Laušević, Sonja, Vuković-Gačić, Branka, "Do ethanolic extracts of Lamiaceae species used in folk medicine have antibiofilm activity on Pseudomonas aeruginosa PAO1?" in Electronic abstract book: FEMS Conference on Microbiology in association with Serbian Society of Microbiology; 2022 Jun 30 - Jul 2; Belgrade, Serbia (2022):801-802,
https://hdl.handle.net/21.15107/rcub_ibiss_5729 .

TY  - CONF
AU  - Mandić, Marija R.
AU  - Oalđe, Mariana M.
AU  - Lunić, Tanja M.
AU  - Sabovljević, Aneta D.
AU  - Sabovljević, Marko S.
AU  - Gašić, Uroš
AU  - Duletić Laušević, Sonja N.
AU  - Božić, Bojan D.
AU  - Božić Nedeljković, Biljana D.
PY  - 2021
UR  - https://onlinelibrary.wiley.com/toc/15214141/2021/51/S1
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4881
AB  - ‚In this study, we aimed to examine the immunomodulatory potential (anti‐neuroinflammatory/neuroprotective and antitumor activities) of various seasonal aspects (spring, summer,
autumn) of moss Hypnum cupressiforme extracts. Moss extracts have been obtained using solvents of different polarities and their mixtures applying Soxhlet extractor. Activities
of the extracts were tested on cell lines MRC‐5, BV2, SH‐SY5Y, HCT‐116, and MDA‐MB‐231, for potential anti‐neuroinflammatory and antitumor activities. The impact of extracts on
cell viability/metabolic activity was measured by MTT assay, while the influence on the production of ROS and NO was determined using NBT and Griess assays, respectively. The
biocompatibility of extracts was confirmed using MRC‐5 cells. Extracts of moss from spring season have shown significant antiproliferative activity against MDA‐MB‐231 cell line
(inhibition rate ~50%), as well as anti‐neuroinflammatory activity. Furthermore, the effects of seasonal variation (spring, summer, autumn) on the immunomodulatory potential of
ethyl‐acetate extract were examined. Summer aspect of moss has led to the greatest reduction of ROS production, while autumn aspect had the greatest impact on the reduction of NO
production by LPS‐activated BV2 cells. Additionally, the supernatant‐transfer model proved that treatment with moss extracts from summer and autumn seasons of LPS‐activated BV2
cells exhibit neuroprotective activity towards SH‐SY5Y neurons. The obtained data suggest that extracts from moss H. cupressiforme possess significant immunomodulatory potential
evaluated in vitro, which may be useful in conditions such as Alzheimer’s disease, Parkinson’s disease, and breast cancer. In terms of anti‐neuroinflammatory/neuroprotective activity,
summer and autumn were characterized as the most favorable seasons.
PB  - Wiley‐VCH GmbH
C3  - 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
T1  - Immunomodulatory properties of extracts of moss Hypnum cupressiforme from various seasons
DO  - 10.1002/eji.202170200
SP  - 296
ER  - 

@conference{
author = "Mandić, Marija R. and Oalđe, Mariana M. and Lunić, Tanja M. and Sabovljević, Aneta D. and Sabovljević, Marko S. and Gašić, Uroš and Duletić Laušević, Sonja N. and Božić, Bojan D. and Božić Nedeljković, Biljana D.",
year = "2021",
abstract = "‚In this study, we aimed to examine the immunomodulatory potential (anti‐neuroinflammatory/neuroprotective and antitumor activities) of various seasonal aspects (spring, summer,
autumn) of moss Hypnum cupressiforme extracts. Moss extracts have been obtained using solvents of different polarities and their mixtures applying Soxhlet extractor. Activities
of the extracts were tested on cell lines MRC‐5, BV2, SH‐SY5Y, HCT‐116, and MDA‐MB‐231, for potential anti‐neuroinflammatory and antitumor activities. The impact of extracts on
cell viability/metabolic activity was measured by MTT assay, while the influence on the production of ROS and NO was determined using NBT and Griess assays, respectively. The
biocompatibility of extracts was confirmed using MRC‐5 cells. Extracts of moss from spring season have shown significant antiproliferative activity against MDA‐MB‐231 cell line
(inhibition rate ~50%), as well as anti‐neuroinflammatory activity. Furthermore, the effects of seasonal variation (spring, summer, autumn) on the immunomodulatory potential of
ethyl‐acetate extract were examined. Summer aspect of moss has led to the greatest reduction of ROS production, while autumn aspect had the greatest impact on the reduction of NO
production by LPS‐activated BV2 cells. Additionally, the supernatant‐transfer model proved that treatment with moss extracts from summer and autumn seasons of LPS‐activated BV2
cells exhibit neuroprotective activity towards SH‐SY5Y neurons. The obtained data suggest that extracts from moss H. cupressiforme possess significant immunomodulatory potential
evaluated in vitro, which may be useful in conditions such as Alzheimer’s disease, Parkinson’s disease, and breast cancer. In terms of anti‐neuroinflammatory/neuroprotective activity,
summer and autumn were characterized as the most favorable seasons.",
publisher = "Wiley‐VCH GmbH",
journal = "6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting",
title = "Immunomodulatory properties of extracts of moss Hypnum cupressiforme from various seasons",
doi = "10.1002/eji.202170200",
pages = "296"
}

Mandić, M. R., Oalđe, M. M., Lunić, T. M., Sabovljević, A. D., Sabovljević, M. S., Gašić, U., Duletić Laušević, S. N., Božić, B. D.,& Božić Nedeljković, B. D.. (2021). Immunomodulatory properties of extracts of moss Hypnum cupressiforme from various seasons. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
Wiley‐VCH GmbH., 296.
https://doi.org/10.1002/eji.202170200

Mandić MR, Oalđe MM, Lunić TM, Sabovljević AD, Sabovljević MS, Gašić U, Duletić Laušević SN, Božić BD, Božić Nedeljković BD. Immunomodulatory properties of extracts of moss Hypnum cupressiforme from various seasons. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. 2021;:296.
doi:10.1002/eji.202170200 .

Mandić, Marija R., Oalđe, Mariana M., Lunić, Tanja M., Sabovljević, Aneta D., Sabovljević, Marko S., Gašić, Uroš, Duletić Laušević, Sonja N., Božić, Bojan D., Božić Nedeljković, Biljana D., "Immunomodulatory properties of extracts of moss Hypnum cupressiforme from various seasons" in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting (2021):296,
https://doi.org/10.1002/eji.202170200 . .

TY  - CONF
AU  - Oalde, Mariana M.
AU  - Mandić, Marija R.
AU  - Sabovljević, Aneta D.
AU  - Sabovljević, Marko S.
AU  - Gašić, Uroš
AU  - Duletić Laušević, Sonja N.
AU  - Božić, Bojan D.
AU  - Božić Nedeljković, Biljana D.
PY  - 2021
UR  - https://onlinelibrary.wiley.com/toc/15214141/2021/51/S1
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4879
AB  - In this study, we aimed to examine the chemical composition and biochemical activities (antioxidant, antidiabetic, and antineurodegenerative) of seasonal aspects (spring, summer, autumn) of moss Hypnum cupressiforme extracts. The extracts were prepared using Soxhlet extractor. Chemical characterization of extracts was performed via spectrophotometric assays and LC‐MS. The antioxidant activity of the extracts was determined by DPPH (2,2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate), total reduction power, and β‐carotene bleaching assays. Extracts were analyzed for their inhibitory activity on α‐glucosidase, α‐amylase, acetylcholinesterase, and tyrosinase. The chemical analysis of the moss extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. The highest concentration of these compounds was found in the moss from summer season, and was significantly higher in comparison to the spring and autumn aspects. Major compounds identified by LC‐MS in H. cupressiforme extracts were kaempferol, p‐hydroxybenzoic, protocatechuic, p‐coumaric, gallic, and caffeic acid. According to biochemical assays investigated extracts exhibited significant antineurodegenerative potential, evaluated by the capacity to inhibit acetylcholinesterase and tyrosinase. Among the examined aspects, summer and autumn were found particularly efficient, especially at the lowest tested concentrations. Regarding β‐carotene bleaching test, the summer aspect showed the best activity at the highest concentration, while the spring aspect was found more efficient at the lowest investigated concentration. The obtained data suggest that the most favorable season in terms of chemical composition and biochemical characteristics of moss H. cupressiforme is summer. This moss is a promising source of biologically active compounds that may be useful in the treatment of various pathological conditions.
PB  - Wiley‐VCH GmbH
C3  - 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
T1  - (Bio)Chemical characterization of moss Hypnum cupressiforme extracts as potential immunomodulators
DO  - 10.1002/eji.202170200
SP  - 296
ER  - 

@conference{
author = "Oalde, Mariana M. and Mandić, Marija R. and Sabovljević, Aneta D. and Sabovljević, Marko S. and Gašić, Uroš and Duletić Laušević, Sonja N. and Božić, Bojan D. and Božić Nedeljković, Biljana D.",
year = "2021",
abstract = "In this study, we aimed to examine the chemical composition and biochemical activities (antioxidant, antidiabetic, and antineurodegenerative) of seasonal aspects (spring, summer, autumn) of moss Hypnum cupressiforme extracts. The extracts were prepared using Soxhlet extractor. Chemical characterization of extracts was performed via spectrophotometric assays and LC‐MS. The antioxidant activity of the extracts was determined by DPPH (2,2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate), total reduction power, and β‐carotene bleaching assays. Extracts were analyzed for their inhibitory activity on α‐glucosidase, α‐amylase, acetylcholinesterase, and tyrosinase. The chemical analysis of the moss extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. The highest concentration of these compounds was found in the moss from summer season, and was significantly higher in comparison to the spring and autumn aspects. Major compounds identified by LC‐MS in H. cupressiforme extracts were kaempferol, p‐hydroxybenzoic, protocatechuic, p‐coumaric, gallic, and caffeic acid. According to biochemical assays investigated extracts exhibited significant antineurodegenerative potential, evaluated by the capacity to inhibit acetylcholinesterase and tyrosinase. Among the examined aspects, summer and autumn were found particularly efficient, especially at the lowest tested concentrations. Regarding β‐carotene bleaching test, the summer aspect showed the best activity at the highest concentration, while the spring aspect was found more efficient at the lowest investigated concentration. The obtained data suggest that the most favorable season in terms of chemical composition and biochemical characteristics of moss H. cupressiforme is summer. This moss is a promising source of biologically active compounds that may be useful in the treatment of various pathological conditions.",
publisher = "Wiley‐VCH GmbH",
journal = "6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting",
title = "(Bio)Chemical characterization of moss Hypnum cupressiforme extracts as potential immunomodulators",
doi = "10.1002/eji.202170200",
pages = "296"
}

Oalde, M. M., Mandić, M. R., Sabovljević, A. D., Sabovljević, M. S., Gašić, U., Duletić Laušević, S. N., Božić, B. D.,& Božić Nedeljković, B. D.. (2021). (Bio)Chemical characterization of moss Hypnum cupressiforme extracts as potential immunomodulators. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
Wiley‐VCH GmbH., 296.
https://doi.org/10.1002/eji.202170200

Oalde MM, Mandić MR, Sabovljević AD, Sabovljević MS, Gašić U, Duletić Laušević SN, Božić BD, Božić Nedeljković BD. (Bio)Chemical characterization of moss Hypnum cupressiforme extracts as potential immunomodulators. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. 2021;:296.
doi:10.1002/eji.202170200 .

Oalde, Mariana M., Mandić, Marija R., Sabovljević, Aneta D., Sabovljević, Marko S., Gašić, Uroš, Duletić Laušević, Sonja N., Božić, Bojan D., Božić Nedeljković, Biljana D., "(Bio)Chemical characterization of moss Hypnum cupressiforme extracts as potential immunomodulators" in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting (2021):296,
https://doi.org/10.1002/eji.202170200 . .