TY  - JOUR
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Milenkovic, Dragan
AU  - Cesar, Thais
AU  - Filipović R., Miloš
AU  - Milošević, Verica
PY  - 2024
UR  - https://iubmb.onlinelibrary.wiley.com/journal/18728081
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6462
AB  - Citrus flavanones are recognized as promising bioactives within the concept of healthy aging. Thus, the present study investigated the effects of a nutritionally relevant dose of lemon extract (LE) on liver redox regulation and persulfidation levels in 24-month-old Wistar rats. LE (40 mg/kg b.m.) was administered orally once daily for four weeks. Control groups received either vehicle (sunflower oil) or remained intact. The applied methodology considered qPCR, Western blot, protein persulfidation levels evaluation, histochemistry in line with immunofluorescence, liver biochemical assays (glutathione, total -SH groups and malonaldehyde; MDA), liver enzymes in serum and in silico analysis to explore the potential interaction/binding between the proteins studied in the paper. Our results showed that LE increased glutathione peroxidase (GPx), reductase (GR), glutamate-cysteine ligase catalytic and modifier subunit, respectively, as well as Nrf2 gene expressions, but decreased the expression of superoxide dismutase 2 (SOD2). Upon LE application, protein expression showed upregulation of NRF2, SOD2, GPx, GR, and thioredoxin 1 (Trx1). LE significantly decreased the protein persulfidation levels and concentration of MDA, a marker of oxidative damage in the cell. Histological analysis showed a normal liver histoarchitecture without pathological changes, aligning with the normal serum level of hepatic enzymes. Obtained results showed that LE, by modulating hepatic redox regulators Nrf2 and Trx1, diminishes oxidative stress and alters the persulfidation levels, suggesting a considerable beneficial antioxidant potential of lemon flavanones in the old-aged liver.
PB  - John Wiley and Sons
T2  - Biofactors
T1  - Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats
DO  - 10.1002/biof.2038
ER  - 

@article{
author = "Miler, Marko and Živanović, Jasmina and Ajdžanović, Vladimir and Milenkovic, Dragan and Cesar, Thais and Filipović R., Miloš and Milošević, Verica",
year = "2024",
abstract = "Citrus flavanones are recognized as promising bioactives within the concept of healthy aging. Thus, the present study investigated the effects of a nutritionally relevant dose of lemon extract (LE) on liver redox regulation and persulfidation levels in 24-month-old Wistar rats. LE (40 mg/kg b.m.) was administered orally once daily for four weeks. Control groups received either vehicle (sunflower oil) or remained intact. The applied methodology considered qPCR, Western blot, protein persulfidation levels evaluation, histochemistry in line with immunofluorescence, liver biochemical assays (glutathione, total -SH groups and malonaldehyde; MDA), liver enzymes in serum and in silico analysis to explore the potential interaction/binding between the proteins studied in the paper. Our results showed that LE increased glutathione peroxidase (GPx), reductase (GR), glutamate-cysteine ligase catalytic and modifier subunit, respectively, as well as Nrf2 gene expressions, but decreased the expression of superoxide dismutase 2 (SOD2). Upon LE application, protein expression showed upregulation of NRF2, SOD2, GPx, GR, and thioredoxin 1 (Trx1). LE significantly decreased the protein persulfidation levels and concentration of MDA, a marker of oxidative damage in the cell. Histological analysis showed a normal liver histoarchitecture without pathological changes, aligning with the normal serum level of hepatic enzymes. Obtained results showed that LE, by modulating hepatic redox regulators Nrf2 and Trx1, diminishes oxidative stress and alters the persulfidation levels, suggesting a considerable beneficial antioxidant potential of lemon flavanones in the old-aged liver.",
publisher = "John Wiley and Sons",
journal = "Biofactors",
title = "Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats",
doi = "10.1002/biof.2038"
}

Miler, M., Živanović, J., Ajdžanović, V., Milenkovic, D., Cesar, T., Filipović R., M.,& Milošević, V.. (2024). Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats. in Biofactors
John Wiley and Sons..
https://doi.org/10.1002/biof.2038

Miler M, Živanović J, Ajdžanović V, Milenkovic D, Cesar T, Filipović R. M, Milošević V. Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats. in Biofactors. 2024;.
doi:10.1002/biof.2038 .

Miler, Marko, Živanović, Jasmina, Ajdžanović, Vladimir, Milenkovic, Dragan, Cesar, Thais, Filipović R., Miloš, Milošević, Verica, "Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats" in Biofactors (2024),
https://doi.org/10.1002/biof.2038 . .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Čukuranović-Kokoris, Jovana
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Milošević, Verica
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6405
AB  - Uvod. Aplikacija daidzeina može predstavljati efikasnu i manje rizičnu altemativu indikovanoj, klasičnoj estrogenizaciji starijih muškaraca. Cilj ove studije je podrazumevao morfofiziološku analizu kore nadbubrežne žlezde u pacovskom modelu uznapredovale andropauze, nakon primene daidzeina.
Materijal i metode. Sredovečni (16 meseci stari) pacovi Wistar soja su razdeljeni u
lažno operisanu (SO; n=8), orhidektomisanu (Orx; n=8) i daidzeinom tretiranu orhidektomisanu (Orx+D; n=8) grupu. Daidzein (30 mg/kg t.m./dan) je subkutano davan tri nedelje, dok su SO i Orx grupa dobijale samo rastvarač tokom istog perioda. Ciljevi su realizovani korišćenjem stereologije, histohemije/imunohistohemije, imunoeseja i ultrastruktume analize.
Rezultati. Tretman daidzeinom je značajno uvećao volumene ćelija i jedara zone glomeruloze 
za 32% i 41% tim redom, ali je i snizio (p<0.05) nivo cirkulišućeg aldosterona za 61%, sve u 
poređenju sa odgovarajućim parametrima kod Orx zivotinja. Takođe, daidzein je značajno smanjio 
volumen jedara ćelija zone fascikulate, kao i tkivni i cirkulišući nivo kortikosterona, za 24%, 48% i 33% 
navedenim redom, te izrazito (p<0.05) povisio cirkulišući nivo DHEA za oko 4.6 puta, sve u 
poređenju sa odgovarajućim parametrima kod Orx pacova. Daidzeinom prouzrokovano preusmeravanje 
puteva kortikosteroidogeneze ka produkciji adrenalnih androgena bilo je u skladu sa ultrastruktumim nalazom i rearanžmanima kapilarne rnreže u okviru konkretnih zona kore nadbubrežne zlezde.
Zaključak. Imajući u vidu sklad efekata daidzeina u ispitivanom kontekstu, kao relativnu bezbednost njegove upotrebe, moglo bi se reći da daidzein ima perspektivu u tretrnanu starenjem uzorokovanog gubitka androgena, te hiperfunkcije hipotalamo-hipofizno-adrenalne ose i povezanih metaboličkih poremećaja kod muškaraca.
AB  - Introduction. Daidzein application may represent effective and less harmful alternative to indicated, classical estrogenization of the aging men. The aim of this study was to perform morphophysiological analysis of the adrenal cortex, after daidzein supplementation in a rat model of the severe andropause.
Material and methods. Middle-aged (16 months old) Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and daidzein treated orchidectomized (Orx+D; n=8) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis.
Results. Daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei by 32% and 41% respectively, but decreased (p<0.05) circulating aldosterone levels by 61%, all in comparison with the adequate parameters in Orx animals. Also, daidzein significantly decreased zona fasciculata cell nuclei volume, as well as both the adrenal and circulating levels of corticosterone, by 24%, 48% and 33% respectively, but strongly (p<0.05) increased the circulating level of DHEA by about 4.6 times, all when compared to the corresponding parameters of Orx rats. Daidzein-induced shunting of the corticosteroidogenesis pathways towards adrenal androgens production corresponded to the ultrastructural findings and zonal capillary network rearrangements.
Conclusion. Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of aging-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.
PB  - Niš: University of Niš, Faculty of Medicine
C3  - Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
T1  - Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze
T1  - Morphophysiology of the adrenal cortex after daidzein application in a rat model of the severe andropause
SP  - 166
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6405
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Čukuranović-Kokoris, Jovana and Miler, Marko and Šošić-Jurjević, Branka and Živanović, Jasmina and Marković Filipović, Jelena and Milošević, Verica and Filipović, Branko",
year = "2023",
abstract = "Uvod. Aplikacija daidzeina može predstavljati efikasnu i manje rizičnu altemativu indikovanoj, klasičnoj estrogenizaciji starijih muškaraca. Cilj ove studije je podrazumevao morfofiziološku analizu kore nadbubrežne žlezde u pacovskom modelu uznapredovale andropauze, nakon primene daidzeina.
Materijal i metode. Sredovečni (16 meseci stari) pacovi Wistar soja su razdeljeni u
lažno operisanu (SO; n=8), orhidektomisanu (Orx; n=8) i daidzeinom tretiranu orhidektomisanu (Orx+D; n=8) grupu. Daidzein (30 mg/kg t.m./dan) je subkutano davan tri nedelje, dok su SO i Orx grupa dobijale samo rastvarač tokom istog perioda. Ciljevi su realizovani korišćenjem stereologije, histohemije/imunohistohemije, imunoeseja i ultrastruktume analize.
Rezultati. Tretman daidzeinom je značajno uvećao volumene ćelija i jedara zone glomeruloze 
za 32% i 41% tim redom, ali je i snizio (p<0.05) nivo cirkulišućeg aldosterona za 61%, sve u 
poređenju sa odgovarajućim parametrima kod Orx zivotinja. Takođe, daidzein je značajno smanjio 
volumen jedara ćelija zone fascikulate, kao i tkivni i cirkulišući nivo kortikosterona, za 24%, 48% i 33% 
navedenim redom, te izrazito (p<0.05) povisio cirkulišući nivo DHEA za oko 4.6 puta, sve u 
poređenju sa odgovarajućim parametrima kod Orx pacova. Daidzeinom prouzrokovano preusmeravanje 
puteva kortikosteroidogeneze ka produkciji adrenalnih androgena bilo je u skladu sa ultrastruktumim nalazom i rearanžmanima kapilarne rnreže u okviru konkretnih zona kore nadbubrežne zlezde.
Zaključak. Imajući u vidu sklad efekata daidzeina u ispitivanom kontekstu, kao relativnu bezbednost njegove upotrebe, moglo bi se reći da daidzein ima perspektivu u tretrnanu starenjem uzorokovanog gubitka androgena, te hiperfunkcije hipotalamo-hipofizno-adrenalne ose i povezanih metaboličkih poremećaja kod muškaraca., Introduction. Daidzein application may represent effective and less harmful alternative to indicated, classical estrogenization of the aging men. The aim of this study was to perform morphophysiological analysis of the adrenal cortex, after daidzein supplementation in a rat model of the severe andropause.
Material and methods. Middle-aged (16 months old) Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and daidzein treated orchidectomized (Orx+D; n=8) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis.
Results. Daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei by 32% and 41% respectively, but decreased (p<0.05) circulating aldosterone levels by 61%, all in comparison with the adequate parameters in Orx animals. Also, daidzein significantly decreased zona fasciculata cell nuclei volume, as well as both the adrenal and circulating levels of corticosterone, by 24%, 48% and 33% respectively, but strongly (p<0.05) increased the circulating level of DHEA by about 4.6 times, all when compared to the corresponding parameters of Orx rats. Daidzein-induced shunting of the corticosteroidogenesis pathways towards adrenal androgens production corresponded to the ultrastructural findings and zonal capillary network rearrangements.
Conclusion. Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of aging-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.",
publisher = "Niš: University of Niš, Faculty of Medicine",
journal = "Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia",
title = "Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze, Morphophysiology of the adrenal cortex after daidzein application in a rat model of the severe andropause",
pages = "166-167",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6405"
}

Ajdžanović, V., Čukuranović-Kokoris, J., Miler, M., Šošić-Jurjević, B., Živanović, J., Marković Filipović, J., Milošević, V.,& Filipović, B.. (2023). Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
Niš: University of Niš, Faculty of Medicine., 166-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6405

Ajdžanović V, Čukuranović-Kokoris J, Miler M, Šošić-Jurjević B, Živanović J, Marković Filipović J, Milošević V, Filipović B. Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia. 2023;:166-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6405 .

Ajdžanović, Vladimir, Čukuranović-Kokoris, Jovana, Miler, Marko, Šošić-Jurjević, Branka, Živanović, Jasmina, Marković Filipović, Jelena, Milošević, Verica, Filipović, Branko, "Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze" in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia (2023):166-167,
https://hdl.handle.net/21.15107/rcub_ibiss_6405 .

TY  - CONF
AU  - Čukuranović-Kokoris, Jovana
AU  - Dovenska, Monika
AU  - Ajdžanović, Vladimir
AU  - Marković Filipović, Jelena
AU  - Ugrenović, Slađana
AU  - Kundalić, Braca
AU  - Milošević, Verica
AU  - Popovska-Perčinić, Florina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6278
AB  - Uvod. Faktori sredine, kao što je visoka ili niska temperatura ambijenta, predstavljaju snažne stresore za organizam. Hipofizne ćelije koje luče hormone, posebno somatotropne (GH), intenzivno reaguju na ove faktore. Cilj ove studije bio je da ispita efekat dugotrajnog (60-dnevnog) izlaganja pacova umereno visokoj temperaturi ambijenta (35±1°C) na imunohistomorfometrijske parametre GH ćelija hipofize.
Metode. Eksperiment je izveden na odraslim Wistar pacovima, podeljenim u dve jednake grupe: sedam pacova je kontinuirano bilo izloženo temperaturi 35± l°C tokom 60 dana (eksperimentalna grupa), dok je sedam pacova tokom istog perioda čuvano na 20±2°C (kontrolna grupa). Sve su životinje čuvane u režimu od 12 sati dnevne svetlosti i 12 sati mraka, dok su hranu i vodu mogle da uzimaju ad libitum. GH ćelije vizuelizovane su pomoću imunohistohemijske procedure peroksidaze-antiperoksidaze (PAP). Morfometrijska analiza izvedena je korišćenjem višenamenskog M42 test-sistema.
Rezultati. Nakon 60 dana izlaganja povišenoj ambijentalnoj temperaturi, telesna masa životinja bila je značajno smanjena za 16,4%, u poređenju sa kontrolama. GH ćelije u kontrolnoj i eksperimentalnoj grupi bile su ovalnog oblika i locirane u blizini kapilara. Tamne granule, distribuirane po citoplazmi GH ćelija, bile su sebi svojstvenih univer­zalnih karakteristika u obe grupe. Kod eksperimentalnih pacova, celularni i nukleami volumeni GH ćelija, kao i volumenska gustina GH ćelija, značajno su redukovani za 19,1%, 10,6% i 15,8%, redom, u poređenju sa intaktnim kontrolama.
Zaključak. Izlaganje odraslih pacova muškog pola umereno visokoj ambijentalnoj temperaturi u trajanju od 60 dana dovelo je do smanjenja histomorfometrijskih parametara GH ćelija.
PB  - Niš: University of Niš, Faculty of Medicine
C3  - Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
T1  - Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize
SP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6278
ER  - 

@conference{
author = "Čukuranović-Kokoris, Jovana and Dovenska, Monika and Ajdžanović, Vladimir and Marković Filipović, Jelena and Ugrenović, Slađana and Kundalić, Braca and Milošević, Verica and Popovska-Perčinić, Florina",
year = "2023",
abstract = "Uvod. Faktori sredine, kao što je visoka ili niska temperatura ambijenta, predstavljaju snažne stresore za organizam. Hipofizne ćelije koje luče hormone, posebno somatotropne (GH), intenzivno reaguju na ove faktore. Cilj ove studije bio je da ispita efekat dugotrajnog (60-dnevnog) izlaganja pacova umereno visokoj temperaturi ambijenta (35±1°C) na imunohistomorfometrijske parametre GH ćelija hipofize.
Metode. Eksperiment je izveden na odraslim Wistar pacovima, podeljenim u dve jednake grupe: sedam pacova je kontinuirano bilo izloženo temperaturi 35± l°C tokom 60 dana (eksperimentalna grupa), dok je sedam pacova tokom istog perioda čuvano na 20±2°C (kontrolna grupa). Sve su životinje čuvane u režimu od 12 sati dnevne svetlosti i 12 sati mraka, dok su hranu i vodu mogle da uzimaju ad libitum. GH ćelije vizuelizovane su pomoću imunohistohemijske procedure peroksidaze-antiperoksidaze (PAP). Morfometrijska analiza izvedena je korišćenjem višenamenskog M42 test-sistema.
Rezultati. Nakon 60 dana izlaganja povišenoj ambijentalnoj temperaturi, telesna masa životinja bila je značajno smanjena za 16,4%, u poređenju sa kontrolama. GH ćelije u kontrolnoj i eksperimentalnoj grupi bile su ovalnog oblika i locirane u blizini kapilara. Tamne granule, distribuirane po citoplazmi GH ćelija, bile su sebi svojstvenih univer­zalnih karakteristika u obe grupe. Kod eksperimentalnih pacova, celularni i nukleami volumeni GH ćelija, kao i volumenska gustina GH ćelija, značajno su redukovani za 19,1%, 10,6% i 15,8%, redom, u poređenju sa intaktnim kontrolama.
Zaključak. Izlaganje odraslih pacova muškog pola umereno visokoj ambijentalnoj temperaturi u trajanju od 60 dana dovelo je do smanjenja histomorfometrijskih parametara GH ćelija.",
publisher = "Niš: University of Niš, Faculty of Medicine",
journal = "Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia",
title = "Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize",
pages = "160",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6278"
}

Čukuranović-Kokoris, J., Dovenska, M., Ajdžanović, V., Marković Filipović, J., Ugrenović, S., Kundalić, B., Milošević, V.,& Popovska-Perčinić, F.. (2023). Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
Niš: University of Niš, Faculty of Medicine., 160.
https://hdl.handle.net/21.15107/rcub_ibiss_6278

Čukuranović-Kokoris J, Dovenska M, Ajdžanović V, Marković Filipović J, Ugrenović S, Kundalić B, Milošević V, Popovska-Perčinić F. Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia. 2023;:160.
https://hdl.handle.net/21.15107/rcub_ibiss_6278 .

Čukuranović-Kokoris, Jovana, Dovenska, Monika, Ajdžanović, Vladimir, Marković Filipović, Jelena, Ugrenović, Slađana, Kundalić, Braca, Milošević, Verica, Popovska-Perčinić, Florina, "Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize" in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia (2023):160,
https://hdl.handle.net/21.15107/rcub_ibiss_6278 .

TY  - CONF
AU  - Čukuranović-Kokoris, Jovana
AU  - Đorđević, Miodrag
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Stanković Đorević, Dobrila
AU  - Čukuranović, Rade
AU  - Milošević, Verica
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6229
AB  - Soy isoflavone daidzein exerts some beneficial effects in different ageing-related, chronic diseases and may represent a promising alternative to classical estrogenization of the prostate cancer suffering subjects. The aim of this study was to examine the effects of daidzein on the adrenocortical vascular network and hormonal output in a rat model of the mild andropause. Adult (3 months old) Wistar rats were divided into sham-operated (SO; n=7), orchidectomized (Orx; n=7) and daidzein treated orchidectomized (Orx+D; n=7) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Adrenocortical vascular network properties were identified using Novelli histochemical staining, vascular endothelial growth factor (VEGF) immunostaining and the newCast stereological software. Blood concentrations of aldosterone, corticosterone and DHEAS were measured by immunoassays. Volume densities of blood vessels and VEGF depots within the adrenal cortex were not significantly changed after daidzein treatment, although there was a tendency of decrease of these parameters. On the other hand, daidzein application increased (P<0.05) blood concentration of aldosterone by more than 2.5 times and 41%, in comparison with the same parameter in sham-operated and orchidectomized group, respectively. Corticosterone concentration in daidzein-treated, orchidectomized rats showed a tendency of elevation (P>0.05) compared to the both SO animals and orchidectomy alone. DHEAS blood concentration after daidzein application remained at the level of the Orx group, which in both cases ment elevation (P<0.05) by around 80% in comparison with the same parameter in SO group. We conclude that daidzein significantly increases aldosterone and to some lower extent corticosterone output in a rat model of the mild andropause, without significant rearrangements of the entire adrenal cortex vascular network.
PB  - bioscientifica
C3  - 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey
T1  - Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause
DO  - 10.1530/endoabs.90.EP63
SP  - 394
EP  - 395
ER  - 

@conference{
author = "Čukuranović-Kokoris, Jovana and Đorđević, Miodrag and Miler, Marko and Živanović, Jasmina and Marković Filipović, Jelena and Stanković Đorević, Dobrila and Čukuranović, Rade and Milošević, Verica and Filipović, Branko and Ajdžanović, Vladimir",
year = "2023",
abstract = "Soy isoflavone daidzein exerts some beneficial effects in different ageing-related, chronic diseases and may represent a promising alternative to classical estrogenization of the prostate cancer suffering subjects. The aim of this study was to examine the effects of daidzein on the adrenocortical vascular network and hormonal output in a rat model of the mild andropause. Adult (3 months old) Wistar rats were divided into sham-operated (SO; n=7), orchidectomized (Orx; n=7) and daidzein treated orchidectomized (Orx+D; n=7) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Adrenocortical vascular network properties were identified using Novelli histochemical staining, vascular endothelial growth factor (VEGF) immunostaining and the newCast stereological software. Blood concentrations of aldosterone, corticosterone and DHEAS were measured by immunoassays. Volume densities of blood vessels and VEGF depots within the adrenal cortex were not significantly changed after daidzein treatment, although there was a tendency of decrease of these parameters. On the other hand, daidzein application increased (P<0.05) blood concentration of aldosterone by more than 2.5 times and 41%, in comparison with the same parameter in sham-operated and orchidectomized group, respectively. Corticosterone concentration in daidzein-treated, orchidectomized rats showed a tendency of elevation (P>0.05) compared to the both SO animals and orchidectomy alone. DHEAS blood concentration after daidzein application remained at the level of the Orx group, which in both cases ment elevation (P<0.05) by around 80% in comparison with the same parameter in SO group. We conclude that daidzein significantly increases aldosterone and to some lower extent corticosterone output in a rat model of the mild andropause, without significant rearrangements of the entire adrenal cortex vascular network.",
publisher = "bioscientifica",
journal = "25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey",
title = "Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause",
doi = "10.1530/endoabs.90.EP63",
pages = "394-395"
}

Čukuranović-Kokoris, J., Đorđević, M., Miler, M., Živanović, J., Marković Filipović, J., Stanković Đorević, D., Čukuranović, R., Milošević, V., Filipović, B.,& Ajdžanović, V.. (2023). Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause. in 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey
bioscientifica., 394-395.
https://doi.org/10.1530/endoabs.90.EP63

Čukuranović-Kokoris J, Đorđević M, Miler M, Živanović J, Marković Filipović J, Stanković Đorević D, Čukuranović R, Milošević V, Filipović B, Ajdžanović V. Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause. in 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey. 2023;:394-395.
doi:10.1530/endoabs.90.EP63 .

Čukuranović-Kokoris, Jovana, Đorđević, Miodrag, Miler, Marko, Živanović, Jasmina, Marković Filipović, Jelena, Stanković Đorević, Dobrila, Čukuranović, Rade, Milošević, Verica, Filipović, Branko, Ajdžanović, Vladimir, "Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause" in 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey (2023):394-395,
https://doi.org/10.1530/endoabs.90.EP63 . .

TY  - JOUR
AU  - Marković-Filipović, Jelena
AU  - Miler, Marko
AU  - Kojić, Danijela
AU  - Andrejić Višnjić, Bojana
AU  - Milošević, Verica
AU  - Cukuranovic Kokoris, Jovana
AU  - Đorđević, Miodrag
AU  - Matavulj, Milica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5480
AB  - Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory processes. In addition, AA is formed during processing of starchy food at high temperature. The aim of our study was to examine effects of subchronic AA treatment on adult rat liver using histological, stereological and biochemical methods. Adult male Wistar rats were treated with AA at doses of 25 mg/kg b.w. and 50 mg/kg b.w. for three weeks. Stereological analysis showed decrease of volume density of hepatocyte cytoplasm, and increase of volume density of hepatocyte nuclei and nucleocytoplasmic ratio in AA50mg group. Immunohistochemical analysis of the liver sections showed that treatment with AA50mg increase the percentage of PCNA positive cells, while the percentage of caspase 3 positive cells was not affected by AA. PAS-staining showed that glycogen content in hepatocytes was not affected by AA. Serological examination revealed increase of lipid peroxidation in AA50mg group, while total protein concentration, protein thiol group level, as well as, paraoxonase 1 activity were not changed in AA- exposed animals. Stereological and immunohistochemical analyses of adult liver sections suggest increase of proliferation in AA50mg group, while increase of lipid peroxidation in serum of AA50mg group indicates oxidative stress induction.
T2  - International Journal of Morphology
T1  - Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study
IS  - 6
VL  - 40
DO  - 10.4067/s0717-95022022000601618
SP  - 1618
EP  - 1623
ER  - 

@article{
author = "Marković-Filipović, Jelena and Miler, Marko and Kojić, Danijela and Andrejić Višnjić, Bojana and Milošević, Verica and Cukuranovic Kokoris, Jovana and Đorđević, Miodrag and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory processes. In addition, AA is formed during processing of starchy food at high temperature. The aim of our study was to examine effects of subchronic AA treatment on adult rat liver using histological, stereological and biochemical methods. Adult male Wistar rats were treated with AA at doses of 25 mg/kg b.w. and 50 mg/kg b.w. for three weeks. Stereological analysis showed decrease of volume density of hepatocyte cytoplasm, and increase of volume density of hepatocyte nuclei and nucleocytoplasmic ratio in AA50mg group. Immunohistochemical analysis of the liver sections showed that treatment with AA50mg increase the percentage of PCNA positive cells, while the percentage of caspase 3 positive cells was not affected by AA. PAS-staining showed that glycogen content in hepatocytes was not affected by AA. Serological examination revealed increase of lipid peroxidation in AA50mg group, while total protein concentration, protein thiol group level, as well as, paraoxonase 1 activity were not changed in AA- exposed animals. Stereological and immunohistochemical analyses of adult liver sections suggest increase of proliferation in AA50mg group, while increase of lipid peroxidation in serum of AA50mg group indicates oxidative stress induction.",
journal = "International Journal of Morphology",
title = "Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study",
number = "6",
volume = "40",
doi = "10.4067/s0717-95022022000601618",
pages = "1618-1623"
}

Marković-Filipović, J., Miler, M., Kojić, D., Andrejić Višnjić, B., Milošević, V., Cukuranovic Kokoris, J., Đorđević, M.,& Matavulj, M.. (2022). Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study. in International Journal of Morphology, 40(6), 1618-1623.
https://doi.org/10.4067/s0717-95022022000601618

Marković-Filipović J, Miler M, Kojić D, Andrejić Višnjić B, Milošević V, Cukuranovic Kokoris J, Đorđević M, Matavulj M. Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study. in International Journal of Morphology. 2022;40(6):1618-1623.
doi:10.4067/s0717-95022022000601618 .

Marković-Filipović, Jelena, Miler, Marko, Kojić, Danijela, Andrejić Višnjić, Bojana, Milošević, Verica, Cukuranovic Kokoris, Jovana, Đorđević, Miodrag, Matavulj, Milica, "Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study" in International Journal of Morphology, 40, no. 6 (2022):1618-1623,
https://doi.org/10.4067/s0717-95022022000601618 . .

TY  - JOUR
AU  - Čukuranović-Kokoris, Jovana
AU  - Đorđević, Miodrag
AU  - Jovanović, Ivan
AU  - Kundalić, Braca
AU  - Pavlović, Miljana
AU  - Graovac, Ivana
AU  - Ajdžanović, Vladimir
AU  - Ristić, Nataša
AU  - Marković-Filipović, Jelena
AU  - Milošević, Verica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4978
AB  - Introduction/Objective. In this study, we have pointed out the immunohistomorphometric characteristics of somatotropic (GH) and folliculo-stellate (FS) cells of the human pituitary gland during ageing. Methods. On histological sections of the pituitary gland of 14 male cadavers of different ages, the GH and FS cells were immunohistochemically labeled with corresponding antibodies, monoclonal anti-GH antibody and polyclonal anti-S100 antibody, respectively. Immunopositive GH- and FS-cells were further morphometrically analyzed using ImageJ software. Results. The obtained results of morphometric analysis showed that the surface area of GH cells increased significantly with age. In these cells, the nuclear-cytoplasmic ratio gradually decreased and became significantly higher after the age of 70 years. The volume density of GH cells has not changed during ageing, while in FS cells this parameter significantly increased in the cases older than 70 years. The nuclear-cytoplasmic ratio of GH cells is negatively correlated with the volume density of FS cells. Conclusions. Based on the obtained results, we concluded that hypertrophy of GH and FS cells occurs in men with ageing and that correlation between the morphometric parameters of these two cell types indicates their mutual interaction.
AB  - Увод/Циљ У овој студији смо указали на имунохистоморфометријске карактеристике соматотропних (ГХ) и фоликулостелатних (ФС) ћелија хипофизе човека током старења. Методе На хистолошким пресецима хипофизе 14 мушких кадавера различите старости, ГХ и ФС ћелије су имунохистохемијски обележене одговарајућим антителима (моноклонско анти-ГХ антитело и поликлонско анти-С100 антитело). Имунопозитивне ГХ- и ФС-ћелије су морфометријски анализиране коришћењем ImageJ софтвера. Резултати Добијени резултати морфометријске анализе су показали да се површина коју заузимају ГХ ћелије значајно повећава са старењем. У овим ћелијама нуклеарно-цитоплазматски однос се постепено смањивао и након 70. године старости постао значајно већи. Волуменска густина ГХ ћелија се није мењала током старења, док је код ФС ћелија овај параметар значајно повећан у случајевима старијим од 70 година. Нуклеарно-цитоплазматски однос ГХ ћелија је у негативној корелацији са волуменском густином ФС ћелија. Закључак На основу добијених резултата, закључили смо да се хипертрофија ГХ и ФС ћелија јавља код мушкараца са старењем и да корелација између морфометријских параметара ова два типа ћелија указује на њихову међусобну интеракцију.
PB  - Belgrade: Serbian Medical Society
T2  - Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine
T1  - Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing
T1  - Морфометријска анализа соматотропних и фоликулостелатних ћелија
аденохипофизе човека током старења
IS  - 5-6
VL  - 150
DO  - 10.2298/SARH211214044C
SP  - 274
EP  - 280
ER  - 

@article{
author = "Čukuranović-Kokoris, Jovana and Đorđević, Miodrag and Jovanović, Ivan and Kundalić, Braca and Pavlović, Miljana and Graovac, Ivana and Ajdžanović, Vladimir and Ristić, Nataša and Marković-Filipović, Jelena and Milošević, Verica",
year = "2022",
abstract = "Introduction/Objective. In this study, we have pointed out the immunohistomorphometric characteristics of somatotropic (GH) and folliculo-stellate (FS) cells of the human pituitary gland during ageing. Methods. On histological sections of the pituitary gland of 14 male cadavers of different ages, the GH and FS cells were immunohistochemically labeled with corresponding antibodies, monoclonal anti-GH antibody and polyclonal anti-S100 antibody, respectively. Immunopositive GH- and FS-cells were further morphometrically analyzed using ImageJ software. Results. The obtained results of morphometric analysis showed that the surface area of GH cells increased significantly with age. In these cells, the nuclear-cytoplasmic ratio gradually decreased and became significantly higher after the age of 70 years. The volume density of GH cells has not changed during ageing, while in FS cells this parameter significantly increased in the cases older than 70 years. The nuclear-cytoplasmic ratio of GH cells is negatively correlated with the volume density of FS cells. Conclusions. Based on the obtained results, we concluded that hypertrophy of GH and FS cells occurs in men with ageing and that correlation between the morphometric parameters of these two cell types indicates their mutual interaction., Увод/Циљ У овој студији смо указали на имунохистоморфометријске карактеристике соматотропних (ГХ) и фоликулостелатних (ФС) ћелија хипофизе човека током старења. Методе На хистолошким пресецима хипофизе 14 мушких кадавера различите старости, ГХ и ФС ћелије су имунохистохемијски обележене одговарајућим антителима (моноклонско анти-ГХ антитело и поликлонско анти-С100 антитело). Имунопозитивне ГХ- и ФС-ћелије су морфометријски анализиране коришћењем ImageJ софтвера. Резултати Добијени резултати морфометријске анализе су показали да се површина коју заузимају ГХ ћелије значајно повећава са старењем. У овим ћелијама нуклеарно-цитоплазматски однос се постепено смањивао и након 70. године старости постао значајно већи. Волуменска густина ГХ ћелија се није мењала током старења, док је код ФС ћелија овај параметар значајно повећан у случајевима старијим од 70 година. Нуклеарно-цитоплазматски однос ГХ ћелија је у негативној корелацији са волуменском густином ФС ћелија. Закључак На основу добијених резултата, закључили смо да се хипертрофија ГХ и ФС ћелија јавља код мушкараца са старењем и да корелација између морфометријских параметара ова два типа ћелија указује на њихову међусобну интеракцију.",
publisher = "Belgrade: Serbian Medical Society",
journal = "Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine",
title = "Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing, Морфометријска анализа соматотропних и фоликулостелатних ћелија
аденохипофизе човека током старења",
number = "5-6",
volume = "150",
doi = "10.2298/SARH211214044C",
pages = "274-280"
}

Čukuranović-Kokoris, J., Đorđević, M., Jovanović, I., Kundalić, B., Pavlović, M., Graovac, I., Ajdžanović, V., Ristić, N., Marković-Filipović, J.,& Milošević, V.. (2022). Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing. in Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine
Belgrade: Serbian Medical Society., 150(5-6), 274-280.
https://doi.org/10.2298/SARH211214044C

Čukuranović-Kokoris J, Đorđević M, Jovanović I, Kundalić B, Pavlović M, Graovac I, Ajdžanović V, Ristić N, Marković-Filipović J, Milošević V. Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing. in Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine. 2022;150(5-6):274-280.
doi:10.2298/SARH211214044C .

Čukuranović-Kokoris, Jovana, Đorđević, Miodrag, Jovanović, Ivan, Kundalić, Braca, Pavlović, Miljana, Graovac, Ivana, Ajdžanović, Vladimir, Ristić, Nataša, Marković-Filipović, Jelena, Milošević, Verica, "Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing" in Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine, 150, no. 5-6 (2022):274-280,
https://doi.org/10.2298/SARH211214044C . .

TY  - JOUR
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Trifunović, Svetlana
AU  - Ristić, Nataša
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka 
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4971
AB  - Thyroid C-cells secrete the hormone calcitonin (CT) which acts as an inhibitor of bone resorption. Our aim was to examine the age-related changes in the structure and function of CT-producing C-cells, using histomorphometric, ultrastructural, and biochemical analyses. We used young adult (3-months-old), middle-aged (16-months-old), and old (24-months-old) male rats. The peroxidase-antiperoxidase method was applied for localization of CT. Stereological analysis was performed using the newCAST stereological software package. Serum samples were analyzed for the determination of CT, testosterone (T), calcium (Ca2+), and phosphorus (P). We found a significant increase in the volume density (Vv) of C-cells in both older groups (p < 0.05). The percentage of smaller volume range C-cells increased (p < 0.0001), while the proportion of greater volume range C-cells decreased (p < 0.05) with ageing. Ultrastructural analysis revealed a larger number of secretory granules in older rats. Serum CT increased (p < 0.001), while serum T and P were reduced (p < 0.01) in older rats. Serum Ca2+ was lower (p < 0.0001) in middle-aged rats compared to young adults. We revealed a 20% incidence of C-cell hyperplasia in older rats and one case of medullary thyroid carcinoma in an old rat. Our findings indicate that the ageing process causes significant histomorphometric changes at the thyroid C-cell level.
PB  - New York: Cambridge University Press
T2  - Microscopy and Microanalysis
T1  - Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats
DO  - 10.1017/S1431927622000721
ER  - 

@article{
author = "Filipović, Branko and Ajdžanović, Vladimir and Živanović, Jasmina and Trifunović, Svetlana and Ristić, Nataša and Milošević, Verica and Šošić-Jurjević, Branka ",
year = "2022",
abstract = "Thyroid C-cells secrete the hormone calcitonin (CT) which acts as an inhibitor of bone resorption. Our aim was to examine the age-related changes in the structure and function of CT-producing C-cells, using histomorphometric, ultrastructural, and biochemical analyses. We used young adult (3-months-old), middle-aged (16-months-old), and old (24-months-old) male rats. The peroxidase-antiperoxidase method was applied for localization of CT. Stereological analysis was performed using the newCAST stereological software package. Serum samples were analyzed for the determination of CT, testosterone (T), calcium (Ca2+), and phosphorus (P). We found a significant increase in the volume density (Vv) of C-cells in both older groups (p < 0.05). The percentage of smaller volume range C-cells increased (p < 0.0001), while the proportion of greater volume range C-cells decreased (p < 0.05) with ageing. Ultrastructural analysis revealed a larger number of secretory granules in older rats. Serum CT increased (p < 0.001), while serum T and P were reduced (p < 0.01) in older rats. Serum Ca2+ was lower (p < 0.0001) in middle-aged rats compared to young adults. We revealed a 20% incidence of C-cell hyperplasia in older rats and one case of medullary thyroid carcinoma in an old rat. Our findings indicate that the ageing process causes significant histomorphometric changes at the thyroid C-cell level.",
publisher = "New York: Cambridge University Press",
journal = "Microscopy and Microanalysis",
title = "Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats",
doi = "10.1017/S1431927622000721"
}

Filipović, B., Ajdžanović, V., Živanović, J., Trifunović, S., Ristić, N., Milošević, V.,& Šošić-Jurjević, B.. (2022). Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats. in Microscopy and Microanalysis
New York: Cambridge University Press..
https://doi.org/10.1017/S1431927622000721

Filipović B, Ajdžanović V, Živanović J, Trifunović S, Ristić N, Milošević V, Šošić-Jurjević B. Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats. in Microscopy and Microanalysis. 2022;.
doi:10.1017/S1431927622000721 .

Filipović, Branko, Ajdžanović, Vladimir, Živanović, Jasmina, Trifunović, Svetlana, Ristić, Nataša, Milošević, Verica, Šošić-Jurjević, Branka , "Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats" in Microscopy and Microanalysis (2022),
https://doi.org/10.1017/S1431927622000721 . .

TY  - JOUR
AU  - Čukuranović Kokoris, Jovana
AU  - Ajdžanović, Vladimir
AU  - Pendovski, Lazo
AU  - Ristić, Nataša
AU  - Milošević, Verica
AU  - Dovenska, Monika
AU  - Popovska-Perčinić, Florina
PY  - 2022
UR  - https://www.sciendo.com/article/10.2478/acve-2022-0001
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4965
AB  - Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.
T2  - Acta Veterinaria
T1  - The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study
IS  - 1
VL  - 72
DO  - 10.2478/acve-2022-0001
SP  - 1
EP  - 15
ER  - 

@article{
author = "Čukuranović Kokoris, Jovana and Ajdžanović, Vladimir and Pendovski, Lazo and Ristić, Nataša and Milošević, Verica and Dovenska, Monika and Popovska-Perčinić, Florina",
year = "2022",
abstract = "Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.",
journal = "Acta Veterinaria",
title = "The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study",
number = "1",
volume = "72",
doi = "10.2478/acve-2022-0001",
pages = "1-15"
}

Čukuranović Kokoris, J., Ajdžanović, V., Pendovski, L., Ristić, N., Milošević, V., Dovenska, M.,& Popovska-Perčinić, F.. (2022). The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study. in Acta Veterinaria, 72(1), 1-15.
https://doi.org/10.2478/acve-2022-0001

Čukuranović Kokoris J, Ajdžanović V, Pendovski L, Ristić N, Milošević V, Dovenska M, Popovska-Perčinić F. The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study. in Acta Veterinaria. 2022;72(1):1-15.
doi:10.2478/acve-2022-0001 .

Čukuranović Kokoris, Jovana, Ajdžanović, Vladimir, Pendovski, Lazo, Ristić, Nataša, Milošević, Verica, Dovenska, Monika, Popovska-Perčinić, Florina, "The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study" in Acta Veterinaria, 72, no. 1 (2022):1-15,
https://doi.org/10.2478/acve-2022-0001 . .

TY  - JOUR
AU  - Živanović, Jasmina
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Stanković, Sanja
AU  - Milošević, Verica
AU  - Filipović, Branko
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S094096022100162X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4499
AB  - Soy isoflavone genistein interplays with numerous physiological or pathophysiological processes during ageing. However, its protective role and underlying mechanisms of action in the regulation of calcium (Ca2+) and phosphate (Pi) homeostasis in an animal model of the andropause are yet to be fully clarified. Wistar male rats (16-month-old) were divided into sham-operated, orchidectomized, orchidectomized estradiol-treated (0.625 mg/kg b.m./day) and orchidectomized genistein-treated (30 mg/kg b.m./day) groups. Treatments were administered subcutaneously for 3 weeks, while the controls received vehicle alone. Estradiol treatment increased the expression level of fibroblast growth factor receptor (FGFR) and parathyroid hormone 1 receptor (PTH1R), and activated mitogen – activated protein kinase kinase 1/2 (MEK 1/2) signaling pathway in the kidneys. Genistein application induced a prominent gene and protein expression of Klotho and downregulated the expression of FGFR and PTH1R in the kidney of andropausal rats. Activation of protein kinase B (Akt) signalling pathway was observed, while MEK 1/2 signaling pathway wasn't altered after genistein treatment. The increase of 25 (OH) vitamin D in the serum and decrease in Ca2+ urine content was observed after genistein application. Our findings strongly suggest genistein as a potent biocompound with beneficial effects on the regulation of Ca2+ and Pi homeostasis, especially during aging process when the balance of mineral metabolism is impaired. These novel data provide closer insights into the physiological roles of genistein in the regulation of mineral homeostasis.
PB  - Elsevier GmbH
T2  - Annals of Anatomy - Anatomischer Anzeiger
T1  - Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause
VL  - 239
DO  - 10.1016/j.aanat.2021.151836
SP  - 151836
ER  - 

@article{
author = "Živanović, Jasmina and Jarić, Ivana and Ajdžanović, Vladimir and Miler, Marko and Stanković, Sanja and Milošević, Verica and Filipović, Branko",
year = "2022",
abstract = "Soy isoflavone genistein interplays with numerous physiological or pathophysiological processes during ageing. However, its protective role and underlying mechanisms of action in the regulation of calcium (Ca2+) and phosphate (Pi) homeostasis in an animal model of the andropause are yet to be fully clarified. Wistar male rats (16-month-old) were divided into sham-operated, orchidectomized, orchidectomized estradiol-treated (0.625 mg/kg b.m./day) and orchidectomized genistein-treated (30 mg/kg b.m./day) groups. Treatments were administered subcutaneously for 3 weeks, while the controls received vehicle alone. Estradiol treatment increased the expression level of fibroblast growth factor receptor (FGFR) and parathyroid hormone 1 receptor (PTH1R), and activated mitogen – activated protein kinase kinase 1/2 (MEK 1/2) signaling pathway in the kidneys. Genistein application induced a prominent gene and protein expression of Klotho and downregulated the expression of FGFR and PTH1R in the kidney of andropausal rats. Activation of protein kinase B (Akt) signalling pathway was observed, while MEK 1/2 signaling pathway wasn't altered after genistein treatment. The increase of 25 (OH) vitamin D in the serum and decrease in Ca2+ urine content was observed after genistein application. Our findings strongly suggest genistein as a potent biocompound with beneficial effects on the regulation of Ca2+ and Pi homeostasis, especially during aging process when the balance of mineral metabolism is impaired. These novel data provide closer insights into the physiological roles of genistein in the regulation of mineral homeostasis.",
publisher = "Elsevier GmbH",
journal = "Annals of Anatomy - Anatomischer Anzeiger",
title = "Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause",
volume = "239",
doi = "10.1016/j.aanat.2021.151836",
pages = "151836"
}

Živanović, J., Jarić, I., Ajdžanović, V., Miler, M., Stanković, S., Milošević, V.,& Filipović, B.. (2022). Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause. in Annals of Anatomy - Anatomischer Anzeiger
Elsevier GmbH., 239, 151836.
https://doi.org/10.1016/j.aanat.2021.151836

Živanović J, Jarić I, Ajdžanović V, Miler M, Stanković S, Milošević V, Filipović B. Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause. in Annals of Anatomy - Anatomischer Anzeiger. 2022;239:151836.
doi:10.1016/j.aanat.2021.151836 .

Živanović, Jasmina, Jarić, Ivana, Ajdžanović, Vladimir, Miler, Marko, Stanković, Sanja, Milošević, Verica, Filipović, Branko, "Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause" in Annals of Anatomy - Anatomischer Anzeiger, 239 (2022):151836,
https://doi.org/10.1016/j.aanat.2021.151836 . .

TY  - JOUR
AU  - Ruskovska, Tatjana
AU  - Budić-Leto, Irena
AU  - Corral-Jara, Karla Fabiola
AU  - Ajdžanović, Vladimir
AU  - Arola-Arnal, Anna
AU  - Bravo, Francisca Isabel
AU  - Deligiannidou, Georgia-Eirini
AU  - Havlik, Jaroslav
AU  - Janeva, Milkica
AU  - Kistanova, Elena
AU  - Kontogiorgis, Christos
AU  - Krga, Irena
AU  - Massaro, Marika
AU  - Miler, Marko
AU  - Harnafi, Hicham
AU  - Milošević, Verica
AU  - Morand, Christine
AU  - Scoditti, Egeria
AU  - Suárez, Manuel
AU  - Vauzour, David
AU  - Milenkovic, Dragan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4977
AB  - Cardiovascular and metabolic disorders present major causes of mortality in the ageing population. Polyphenols present in human diets possess cardiometabolic protective properties, however their underlying molecular mechanisms in humans are still not well identified. Even though preclinical and in vitro studies advocate that these bioactives can modulate gene expression, most studies were performed using targeted approaches. With the objective to decipher the molecular mechanisms underlying polyphenols cardiometabolic preventive properties in humans, we performed integrative multi-omic bioinformatic analyses of published studies which reported improvements of cardiometabolic risk factors following polyphenol intake, together with genomic analyses performed using untargeted approach. We identified 5 studies within our criteria and nearly 5000 differentially expressed genes, both mRNAs and miRNAs, in peripheral blood cells. Integrative bioinformatic analyses (e.g. pathway and gene network analyses, identification of transcription factors, correlation of gene expression profiles with those associated with diseases and drug intake) revealed that these genes are involved in the processes such as cell adhesion and mobility, immune system, metabolism, or cell signaling. We also identified 27 miRNAs known to regulate processes such as cell cytoskeleton, chemotaxis, cell signaling, or cell metabolism. Gene expression profiles negatively correlated with expression profiles of cardiovascular disease patients, while a positive correlation was observed with gene expression profiles following intake of drugs against cardiometabolic disorders. These analyses further advocate for health protective effects of these bioactives against age-associated diseases. In conclusion, polyphenols can exert multi-genomic modifications in humans and use of untargeted methods coupled with bioinformatic analyses represent the best approach to decipher molecular mechanisms underlying healthy-ageing effects of these bioactives.
PB  - Elsevier B.V.
T2  - Ageing Research Reviews
T1  - Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies
VL  - 79:101649
DO  - 10.1016/j.arr.2022.101649
ER  - 

@article{
author = "Ruskovska, Tatjana and Budić-Leto, Irena and Corral-Jara, Karla Fabiola and Ajdžanović, Vladimir and Arola-Arnal, Anna and Bravo, Francisca Isabel and Deligiannidou, Georgia-Eirini and Havlik, Jaroslav and Janeva, Milkica and Kistanova, Elena and Kontogiorgis, Christos and Krga, Irena and Massaro, Marika and Miler, Marko and Harnafi, Hicham and Milošević, Verica and Morand, Christine and Scoditti, Egeria and Suárez, Manuel and Vauzour, David and Milenkovic, Dragan",
year = "2022",
abstract = "Cardiovascular and metabolic disorders present major causes of mortality in the ageing population. Polyphenols present in human diets possess cardiometabolic protective properties, however their underlying molecular mechanisms in humans are still not well identified. Even though preclinical and in vitro studies advocate that these bioactives can modulate gene expression, most studies were performed using targeted approaches. With the objective to decipher the molecular mechanisms underlying polyphenols cardiometabolic preventive properties in humans, we performed integrative multi-omic bioinformatic analyses of published studies which reported improvements of cardiometabolic risk factors following polyphenol intake, together with genomic analyses performed using untargeted approach. We identified 5 studies within our criteria and nearly 5000 differentially expressed genes, both mRNAs and miRNAs, in peripheral blood cells. Integrative bioinformatic analyses (e.g. pathway and gene network analyses, identification of transcription factors, correlation of gene expression profiles with those associated with diseases and drug intake) revealed that these genes are involved in the processes such as cell adhesion and mobility, immune system, metabolism, or cell signaling. We also identified 27 miRNAs known to regulate processes such as cell cytoskeleton, chemotaxis, cell signaling, or cell metabolism. Gene expression profiles negatively correlated with expression profiles of cardiovascular disease patients, while a positive correlation was observed with gene expression profiles following intake of drugs against cardiometabolic disorders. These analyses further advocate for health protective effects of these bioactives against age-associated diseases. In conclusion, polyphenols can exert multi-genomic modifications in humans and use of untargeted methods coupled with bioinformatic analyses represent the best approach to decipher molecular mechanisms underlying healthy-ageing effects of these bioactives.",
publisher = "Elsevier B.V.",
journal = "Ageing Research Reviews",
title = "Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies",
volume = "79:101649",
doi = "10.1016/j.arr.2022.101649"
}

Ruskovska, T., Budić-Leto, I., Corral-Jara, K. F., Ajdžanović, V., Arola-Arnal, A., Bravo, F. I., Deligiannidou, G., Havlik, J., Janeva, M., Kistanova, E., Kontogiorgis, C., Krga, I., Massaro, M., Miler, M., Harnafi, H., Milošević, V., Morand, C., Scoditti, E., Suárez, M., Vauzour, D.,& Milenkovic, D.. (2022). Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies. in Ageing Research Reviews
Elsevier B.V.., 79:101649.
https://doi.org/10.1016/j.arr.2022.101649

Ruskovska T, Budić-Leto I, Corral-Jara KF, Ajdžanović V, Arola-Arnal A, Bravo FI, Deligiannidou G, Havlik J, Janeva M, Kistanova E, Kontogiorgis C, Krga I, Massaro M, Miler M, Harnafi H, Milošević V, Morand C, Scoditti E, Suárez M, Vauzour D, Milenkovic D. Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies. in Ageing Research Reviews. 2022;79:101649.
doi:10.1016/j.arr.2022.101649 .

Ruskovska, Tatjana, Budić-Leto, Irena, Corral-Jara, Karla Fabiola, Ajdžanović, Vladimir, Arola-Arnal, Anna, Bravo, Francisca Isabel, Deligiannidou, Georgia-Eirini, Havlik, Jaroslav, Janeva, Milkica, Kistanova, Elena, Kontogiorgis, Christos, Krga, Irena, Massaro, Marika, Miler, Marko, Harnafi, Hicham, Milošević, Verica, Morand, Christine, Scoditti, Egeria, Suárez, Manuel, Vauzour, David, Milenkovic, Dragan, "Systematic analysis of nutrigenomic effects of polyphenols related to cardiometabolic health in humans - Evidence from untargeted mRNA and miRNA studies" in Ageing Research Reviews, 79:101649 (2022),
https://doi.org/10.1016/j.arr.2022.101649 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Milenković, Dragan
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4144
AB  - In a series of our previous works, we revealed the beneficial effects of applied soy isoflavones (genistein or
daidzein) on the wide context of corticosteroidogenesis in vivo, in a rat model of the andropause. Soy isoflavones
decreased the circulating levels of pituitary adrenocorticotropic hormone, inhibited aldosterone secretion, as
well as corticosterone production and secretion, but stimulated dehydroepiandrosterone secretion, all in
andropausal rats. In vitro studies indicate that the mechanism underlying these hormonal changes relies on inhibition of the pituitary tyrosine kinase and adrenocortical 3β-hydroxysteroid dehydrogenase enzymes by soy
isoflavones. Although the clinical studies are in their infancy, the opinion is that genistein and daidzein have
therapeutic potential for the safe treatment of ageing-caused androgen deprivation and glucocorticoid excess
with related metabolic/hemodynamic issues in males. Our accumulated experience and knowledge in the field of
biomedical effects of plant polyphenols have provided a platform for potential recommending the agenda to
organize and accelerate experimental research aimed at producing the optimal supplementation. We hypothesize
that an in vivo approach should first be exploited in the sequence of investigative steps, followed by in vitro
studies and synchronously conducted molecular docking analyses. In vivo research, besides establishing the
margin of exposure safety or adjustment of the correct polyphenol dose, enables identification and quantification
of the metabolites of applied polyphenols in the blood. Subsequent in vitro exploitation of the metabolites and
related docking analyses provide clarification of the molecular mechanisms of action of applied polyphenols.
Chemical modification of the polyphenol structure or coupling it with nanoparticles might be the next step in
optimizing the design of supplementation. Selected, intact or chemically-modified polyphenol molecules should
be included in preclinical studies on a more closely-related species, while clinical studies would finally assess the
safety and effectiveness of a polyphenol-based remedial strategy. The final supplement represents a product of an
appropriate technological process, conducted in accordance with the recommendations derived from the preceding research.
PB  - Elsevir
T2  - Medical Hypotheses
T1  - Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design
VL  - 148
DO  - 10.1016/j.mehy.2021.110516
SP  - 110516
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka  and Filipović, Branko and Milenković, Dragan and Jakovljević, Vladimir and Milošević, Verica",
year = "2021",
abstract = "In a series of our previous works, we revealed the beneficial effects of applied soy isoflavones (genistein or
daidzein) on the wide context of corticosteroidogenesis in vivo, in a rat model of the andropause. Soy isoflavones
decreased the circulating levels of pituitary adrenocorticotropic hormone, inhibited aldosterone secretion, as
well as corticosterone production and secretion, but stimulated dehydroepiandrosterone secretion, all in
andropausal rats. In vitro studies indicate that the mechanism underlying these hormonal changes relies on inhibition of the pituitary tyrosine kinase and adrenocortical 3β-hydroxysteroid dehydrogenase enzymes by soy
isoflavones. Although the clinical studies are in their infancy, the opinion is that genistein and daidzein have
therapeutic potential for the safe treatment of ageing-caused androgen deprivation and glucocorticoid excess
with related metabolic/hemodynamic issues in males. Our accumulated experience and knowledge in the field of
biomedical effects of plant polyphenols have provided a platform for potential recommending the agenda to
organize and accelerate experimental research aimed at producing the optimal supplementation. We hypothesize
that an in vivo approach should first be exploited in the sequence of investigative steps, followed by in vitro
studies and synchronously conducted molecular docking analyses. In vivo research, besides establishing the
margin of exposure safety or adjustment of the correct polyphenol dose, enables identification and quantification
of the metabolites of applied polyphenols in the blood. Subsequent in vitro exploitation of the metabolites and
related docking analyses provide clarification of the molecular mechanisms of action of applied polyphenols.
Chemical modification of the polyphenol structure or coupling it with nanoparticles might be the next step in
optimizing the design of supplementation. Selected, intact or chemically-modified polyphenol molecules should
be included in preclinical studies on a more closely-related species, while clinical studies would finally assess the
safety and effectiveness of a polyphenol-based remedial strategy. The final supplement represents a product of an
appropriate technological process, conducted in accordance with the recommendations derived from the preceding research.",
publisher = "Elsevir",
journal = "Medical Hypotheses",
title = "Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design",
volume = "148",
doi = "10.1016/j.mehy.2021.110516",
pages = "110516"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B., Filipović, B., Milenković, D., Jakovljević, V.,& Milošević, V.. (2021). Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design. in Medical Hypotheses
Elsevir., 148, 110516.
https://doi.org/10.1016/j.mehy.2021.110516

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B, Milenković D, Jakovljević V, Milošević V. Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design. in Medical Hypotheses. 2021;148:110516.
doi:10.1016/j.mehy.2021.110516 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka , Filipović, Branko, Milenković, Dragan, Jakovljević, Vladimir, Milošević, Verica, "Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design" in Medical Hypotheses, 148 (2021):110516,
https://doi.org/10.1016/j.mehy.2021.110516 . .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Ivelja, Ivana
AU  - Karan, Jelena
AU  - Miler, Marko
AU  - Milošević, Verica
AU  - Matavulj, Milica
PY  - 2021
UR  - https://ibsc2021.pmf.uns.ac.rs/
UR  - https://ibsc2021.pmf.uns.ac.rs/ebook-of-abstracts/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6577
AB  - INTRODUCTION: Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory purposes. In addition, AA is formed in many types of fried and oven-baked foods during cooking. Considering proven neurotoxic, carcinogenic and mutagenic effects on living organisms, AA became a main topic of interest for many research groups. OBJECTIVES: The objective of our study was to determine whether acrylamide treatment affects superoxide dismutase 1 (SOD1) expression in rat liver. METHOD / DESIGN: Adult male Wistar rats were subchronicly (three weeks) treated with 25 mg/kg or 50 mg/kg body weight (b.w.) of AA. Formalin-fixed paraffin-embedded liver tissue was cut into 5 µm thin sections and immunostained with anti-SOD1 antibody. The amount of SOD1 in immunostained sections was determined using Windows based ImageJ program (ImageJ, Version 1.50f). The optical density (OD) and stained percentage color area of immunolabeled SOD1 were measured. RESULTS: Figure 1. Representative micrographs of superoxide dismutase 1 (SOD1) immunohistochemical staining in liver of control rats, rats treated with
acrylamide (AA) in dose of 25 mg/kg b.w., and rats treated with acrylamide in dose of 50 mg/kg b.w. (a). Optical density of SOD1 immunopositive cells in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (b). Percentage contribution of high positive, positive, low positive and total positive immunohistochemical staining of SOD1 in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (c). Values in charts are means ± SEM; n = 10, *p < 0.05. In statistical analysis AA-treated animals were compared with the control group. CONCLUSIONS: Immunostaining of SOD1 in liver of control rats showed weak cytoplasmic immunoreactivity in hepatocytes (Fig. 1a). AA application induced dose dependent increase of immunostaining intensity (Fig. 1a). Significant increase of OD and percentage contribution of low positive and total positive cells of immunostained SOD1 was detected in group treated with AA in a dose of 50 mg/kg (Fig.1b, c).
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver
SP  - 137
EP  - 138
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6577
ER  - 

@conference{
author = "Marković Filipović, Jelena and Ivelja, Ivana and Karan, Jelena and Miler, Marko and Milošević, Verica and Matavulj, Milica",
year = "2021",
abstract = "INTRODUCTION: Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory purposes. In addition, AA is formed in many types of fried and oven-baked foods during cooking. Considering proven neurotoxic, carcinogenic and mutagenic effects on living organisms, AA became a main topic of interest for many research groups. OBJECTIVES: The objective of our study was to determine whether acrylamide treatment affects superoxide dismutase 1 (SOD1) expression in rat liver. METHOD / DESIGN: Adult male Wistar rats were subchronicly (three weeks) treated with 25 mg/kg or 50 mg/kg body weight (b.w.) of AA. Formalin-fixed paraffin-embedded liver tissue was cut into 5 µm thin sections and immunostained with anti-SOD1 antibody. The amount of SOD1 in immunostained sections was determined using Windows based ImageJ program (ImageJ, Version 1.50f). The optical density (OD) and stained percentage color area of immunolabeled SOD1 were measured. RESULTS: Figure 1. Representative micrographs of superoxide dismutase 1 (SOD1) immunohistochemical staining in liver of control rats, rats treated with
acrylamide (AA) in dose of 25 mg/kg b.w., and rats treated with acrylamide in dose of 50 mg/kg b.w. (a). Optical density of SOD1 immunopositive cells in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (b). Percentage contribution of high positive, positive, low positive and total positive immunohistochemical staining of SOD1 in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (c). Values in charts are means ± SEM; n = 10, *p < 0.05. In statistical analysis AA-treated animals were compared with the control group. CONCLUSIONS: Immunostaining of SOD1 in liver of control rats showed weak cytoplasmic immunoreactivity in hepatocytes (Fig. 1a). AA application induced dose dependent increase of immunostaining intensity (Fig. 1a). Significant increase of OD and percentage contribution of low positive and total positive cells of immunostained SOD1 was detected in group treated with AA in a dose of 50 mg/kg (Fig.1b, c).",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver",
pages = "137-138",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6577"
}

Marković Filipović, J., Ivelja, I., Karan, J., Miler, M., Milošević, V.,& Matavulj, M.. (2021). Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 137-138.
https://hdl.handle.net/21.15107/rcub_ibiss_6577

Marković Filipović J, Ivelja I, Karan J, Miler M, Milošević V, Matavulj M. Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:137-138.
https://hdl.handle.net/21.15107/rcub_ibiss_6577 .

Marković Filipović, Jelena, Ivelja, Ivana, Karan, Jelena, Miler, Marko, Milošević, Verica, Matavulj, Milica, "Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):137-138,
https://hdl.handle.net/21.15107/rcub_ibiss_6577 .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Kojić, Danijela
AU  - Miler, Marko
AU  - Milošević, Verica
AU  - Matavulj, Milica
PY  - 2021
UR  - https://ibsc2021.pmf.uns.ac.rs/
UR  - https://ibsc2021.pmf.uns.ac.rs/ebook-of-abstracts/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6578
AB  - INTRODUCTION: Acrylamide (AA) is industrial toxic substance with neurotoxic and reprotoxic effects. AA is a Maillard reaction product formed during processing of starchy food at high temperature.
OBJECTIVES: The objective of our study was to determine whether acrylamide treatment disturbs redox balance by altering nitrite, gluthatione (GSH), and malondialdehyde levels in rat hepatoma cell line (H4IIE). METHOD / DESIGN:
Rat hepatoma cell line H4IIE was treated with 4 mM (IC20) and 4.5 mM (IC50) of AA for 24 h. The nitrite level in the medium was analyzed as an indicator of NO production following the Griess reaction method. After ultrasonic cell lysis in 2.5% sulfocalicylic acid, supernatant was analysed for the content of gluthatione. Lipid peroxidation was evaluated using thiobarbituric acid reactive substance assay (TBARS). RESULTS: Detected nitrite, malondialdehyde and GSH levels in rat hepatoma cell line H4IIE after acrylamide treatment are shown in Figure 1. Figure 1. Nitrite concentration (a), malondialdehyde (MDA) concentration (b), reduced glutathione (GSH) concentration (c) in H4IIE cells after treatment with 4 and 4.5 mM acrylamide (AA) for 24 h. Values in charts are means ± SEM of three experiments performed in triplicate. Mean values were significantly different from that of untreated control cells (*p<0.05). CONCLUSIONS: In rat hepatoma cell line H4IIE, exposure to AA caused significant concentration-dependent increase of nitrite level and lipid peroxidation (Fig. 1a, b). On the other hand, GSH content significantly decreased in a concentration-dependent manner in H4IIE cells (Fig.1c). Obtained results indicate that AA disturbs redox status in hepatocytes.
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Acrylamide treatment affects oxidative stress parameters in rat hepatocytes
SP  - 139
EP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6578
ER  - 

@conference{
author = "Marković Filipović, Jelena and Kojić, Danijela and Miler, Marko and Milošević, Verica and Matavulj, Milica",
year = "2021",
abstract = "INTRODUCTION: Acrylamide (AA) is industrial toxic substance with neurotoxic and reprotoxic effects. AA is a Maillard reaction product formed during processing of starchy food at high temperature.
OBJECTIVES: The objective of our study was to determine whether acrylamide treatment disturbs redox balance by altering nitrite, gluthatione (GSH), and malondialdehyde levels in rat hepatoma cell line (H4IIE). METHOD / DESIGN:
Rat hepatoma cell line H4IIE was treated with 4 mM (IC20) and 4.5 mM (IC50) of AA for 24 h. The nitrite level in the medium was analyzed as an indicator of NO production following the Griess reaction method. After ultrasonic cell lysis in 2.5% sulfocalicylic acid, supernatant was analysed for the content of gluthatione. Lipid peroxidation was evaluated using thiobarbituric acid reactive substance assay (TBARS). RESULTS: Detected nitrite, malondialdehyde and GSH levels in rat hepatoma cell line H4IIE after acrylamide treatment are shown in Figure 1. Figure 1. Nitrite concentration (a), malondialdehyde (MDA) concentration (b), reduced glutathione (GSH) concentration (c) in H4IIE cells after treatment with 4 and 4.5 mM acrylamide (AA) for 24 h. Values in charts are means ± SEM of three experiments performed in triplicate. Mean values were significantly different from that of untreated control cells (*p<0.05). CONCLUSIONS: In rat hepatoma cell line H4IIE, exposure to AA caused significant concentration-dependent increase of nitrite level and lipid peroxidation (Fig. 1a, b). On the other hand, GSH content significantly decreased in a concentration-dependent manner in H4IIE cells (Fig.1c). Obtained results indicate that AA disturbs redox status in hepatocytes.",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Acrylamide treatment affects oxidative stress parameters in rat hepatocytes",
pages = "139-140",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6578"
}

Marković Filipović, J., Kojić, D., Miler, M., Milošević, V.,& Matavulj, M.. (2021). Acrylamide treatment affects oxidative stress parameters in rat hepatocytes. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 139-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6578

Marković Filipović J, Kojić D, Miler M, Milošević V, Matavulj M. Acrylamide treatment affects oxidative stress parameters in rat hepatocytes. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:139-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6578 .

Marković Filipović, Jelena, Kojić, Danijela, Miler, Marko, Milošević, Verica, Matavulj, Milica, "Acrylamide treatment affects oxidative stress parameters in rat hepatocytes" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):139-140,
https://hdl.handle.net/21.15107/rcub_ibiss_6578 .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Miler, Marko
AU  - Milošević, Verica
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4223
PB  - Dortmund : IfADo - Leibniz Research Centre for Working Environment and Human Factors
T2  - EXCLI Journal
T1  - Margins of beneficial daily dosage of supplements in prevention of COVID-19
VL  - 20
DO  - 10.17179/excli2021-3790
SP  - 828
EP  - 834
ER  - 

@article{
author = "Ajdžanović, Vladimir and Filipović, Branko and Šošić-Jurjević, Branka  and Miler, Marko and Milošević, Verica",
year = "2021",
publisher = "Dortmund : IfADo - Leibniz Research Centre for Working Environment and Human Factors",
journal = "EXCLI Journal",
title = "Margins of beneficial daily dosage of supplements in prevention of COVID-19",
volume = "20",
doi = "10.17179/excli2021-3790",
pages = "828-834"
}

Ajdžanović, V., Filipović, B., Šošić-Jurjević, B., Miler, M.,& Milošević, V.. (2021). Margins of beneficial daily dosage of supplements in prevention of COVID-19. in EXCLI Journal
Dortmund : IfADo - Leibniz Research Centre for Working Environment and Human Factors., 20, 828-834.
https://doi.org/10.17179/excli2021-3790

Ajdžanović V, Filipović B, Šošić-Jurjević B, Miler M, Milošević V. Margins of beneficial daily dosage of supplements in prevention of COVID-19. in EXCLI Journal. 2021;20:828-834.
doi:10.17179/excli2021-3790 .

Ajdžanović, Vladimir, Filipović, Branko, Šošić-Jurjević, Branka , Miler, Marko, Milošević, Verica, "Margins of beneficial daily dosage of supplements in prevention of COVID-19" in EXCLI Journal, 20 (2021):828-834,
https://doi.org/10.17179/excli2021-3790 . .

TY  - JOUR
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
PY  - 2021
UR  - https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/abs/thyroid-gland-alterations-in-oldaged-wistar-rats-a-comprehensive-stereological-ultrastructural-hormonal-and-gene-expression-study/4C35B9A6AFED404C65272C08C8C2DC27
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4143
AB  - The aim of the present study was to determine and elaborate on all changes in old-aged (OA) versus young-aged (YA) rat thyroids by using
stereological, ultrastructural, hormonal, and gene expression analyses. We used 4- and 24-month-old male Wistar rats in our evaluation,
presenting all changes in comparison with YA rats. Results showed that the thyroid parenchyma was characterized by higher absolute
volumes of the gland, colloid, epithelium, and interstitium by 135, 135, 140, and 142% ( p < 0.05) respectively, while the relative volumes
of colloid and glands were unchanged. Ultrastructural analysis revealed less active glands, with smaller amounts of lysosomes, thyroglobulin
(Tg) granules, and microvilli in the luminal colloid. Optical density values for thyroid peroxidase (TPO), Tg, and vascular-endothelial
growth factor immunostaining remained unchanged; however, TPO and Tg exhibited visually stronger expression in small active follicles.
Thyroxine (T4)-Tg, the relative intensity of fluorescence (RIF), serum T4, and the sodium-iodide symporter immunohistochemical and gene
expressions decreased by 20, 40, 29, and 31% ( p < 0.05), respectively, in OA thyroids. Pituitary thyroid-stimulating hormone (TSH) RIF
increased by 44% ( p < 0.05), but the TSH serum concentration remained unchanged. In conclusion, the obtained results indicate depression
of the thyroid gland synthetic and secretory capacity with advanced age.
PB  - Cambridge University Press
T2  - Microscopy and Microanalysis
T1  - Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study
DO  - 10.1017/S1431927621000064
ER  - 

@article{
author = "Miler, Marko and Ajdžanović, Vladimir and Živanović, Jasmina and Marković Filipović, Jelena and Šošić-Jurjević, Branka  and Milošević, Verica",
year = "2021",
abstract = "The aim of the present study was to determine and elaborate on all changes in old-aged (OA) versus young-aged (YA) rat thyroids by using
stereological, ultrastructural, hormonal, and gene expression analyses. We used 4- and 24-month-old male Wistar rats in our evaluation,
presenting all changes in comparison with YA rats. Results showed that the thyroid parenchyma was characterized by higher absolute
volumes of the gland, colloid, epithelium, and interstitium by 135, 135, 140, and 142% ( p < 0.05) respectively, while the relative volumes
of colloid and glands were unchanged. Ultrastructural analysis revealed less active glands, with smaller amounts of lysosomes, thyroglobulin
(Tg) granules, and microvilli in the luminal colloid. Optical density values for thyroid peroxidase (TPO), Tg, and vascular-endothelial
growth factor immunostaining remained unchanged; however, TPO and Tg exhibited visually stronger expression in small active follicles.
Thyroxine (T4)-Tg, the relative intensity of fluorescence (RIF), serum T4, and the sodium-iodide symporter immunohistochemical and gene
expressions decreased by 20, 40, 29, and 31% ( p < 0.05), respectively, in OA thyroids. Pituitary thyroid-stimulating hormone (TSH) RIF
increased by 44% ( p < 0.05), but the TSH serum concentration remained unchanged. In conclusion, the obtained results indicate depression
of the thyroid gland synthetic and secretory capacity with advanced age.",
publisher = "Cambridge University Press",
journal = "Microscopy and Microanalysis",
title = "Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study",
doi = "10.1017/S1431927621000064"
}

Miler, M., Ajdžanović, V., Živanović, J., Marković Filipović, J., Šošić-Jurjević, B.,& Milošević, V.. (2021). Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study. in Microscopy and Microanalysis
Cambridge University Press..
https://doi.org/10.1017/S1431927621000064

Miler M, Ajdžanović V, Živanović J, Marković Filipović J, Šošić-Jurjević B, Milošević V. Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study. in Microscopy and Microanalysis. 2021;.
doi:10.1017/S1431927621000064 .

Miler, Marko, Ajdžanović, Vladimir, Živanović, Jasmina, Marković Filipović, Jelena, Šošić-Jurjević, Branka , Milošević, Verica, "Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study" in Microscopy and Microanalysis (2021),
https://doi.org/10.1017/S1431927621000064 . .

TY  - JOUR
AU  - Platanić Arizanović, Lena
AU  - Nikolić-Kokić, Aleksandra
AU  - Brkljačić, Jelena
AU  - Tatalović, Nikola
AU  - Miler, Marko
AU  - Oreščanin Dušić, Zorana
AU  - Vidonja Uzelac, Teodora
AU  - Nikolić, Milan
AU  - Milošević, Verica
AU  - Blagojević, Duško
AU  - Spasić, Snežana
AU  - Miljević, Čedo
PY  - 2021
UR  - https://www.tandfonline.com/doi/abs/10.1080/15287394.2020.1844827
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4037
AB  - Chronic use of atypical antipsychotics may produce hepatic damage. Atypical antipsychotics, including clozapine, sertindole, and ziprasidone, are extensively metabolized by the liver and this process generates toxic-free radical metabolic intermediates which may contribute to liver damage. The aim of this study was to investigate whether clozapine, sertindole, or ziprasidone affected hepatic antioxidant defense enzymes which consequently led to disturbed redox homeostasis. The expression and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), and glutathione-S-transferases (GST) were measured in rat livers at doses corresponding to human antipsychotic therapy. Clozapine increased activity of SOD types 1 and 2, GR and GST, but reduced CAT activity. Sertindole elevated activities of both SODs. In ziprasidone-treated rats only decreased CAT activity was found. All three antipsychotics produced mild-to-moderate hepatic histopathological changes categorized as regenerative alterations. No apparent signs of immune cell infiltration, microvesicular or macrovesicular fatty change, or hepatocytes in mitosis were observed. In conclusion, a 4-week long daily treatment with clozapine, sertindole, or ziprasidone altered hepatic antioxidant enzyme activities and induced histopathological changes in liver. The most severe alterations were noted in clozapine-treated rats. Data indicate that redox disturbances may contribute to liver dysfunction after long-term atypical antipsychotic drug treatment.
PB  - Bellwether Publishing, Ltd.
T2  - Journal of Toxicology and Environmental Health, Part A
T1  - Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction
IS  - 4
VL  - 84
DO  - 10.1080/15287394.2020.1844827
SP  - 173
EP  - 182
ER  - 

@article{
author = "Platanić Arizanović, Lena and Nikolić-Kokić, Aleksandra and Brkljačić, Jelena and Tatalović, Nikola and Miler, Marko and Oreščanin Dušić, Zorana and Vidonja Uzelac, Teodora and Nikolić, Milan and Milošević, Verica and Blagojević, Duško and Spasić, Snežana and Miljević, Čedo",
year = "2021",
abstract = "Chronic use of atypical antipsychotics may produce hepatic damage. Atypical antipsychotics, including clozapine, sertindole, and ziprasidone, are extensively metabolized by the liver and this process generates toxic-free radical metabolic intermediates which may contribute to liver damage. The aim of this study was to investigate whether clozapine, sertindole, or ziprasidone affected hepatic antioxidant defense enzymes which consequently led to disturbed redox homeostasis. The expression and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), and glutathione-S-transferases (GST) were measured in rat livers at doses corresponding to human antipsychotic therapy. Clozapine increased activity of SOD types 1 and 2, GR and GST, but reduced CAT activity. Sertindole elevated activities of both SODs. In ziprasidone-treated rats only decreased CAT activity was found. All three antipsychotics produced mild-to-moderate hepatic histopathological changes categorized as regenerative alterations. No apparent signs of immune cell infiltration, microvesicular or macrovesicular fatty change, or hepatocytes in mitosis were observed. In conclusion, a 4-week long daily treatment with clozapine, sertindole, or ziprasidone altered hepatic antioxidant enzyme activities and induced histopathological changes in liver. The most severe alterations were noted in clozapine-treated rats. Data indicate that redox disturbances may contribute to liver dysfunction after long-term atypical antipsychotic drug treatment.",
publisher = "Bellwether Publishing, Ltd.",
journal = "Journal of Toxicology and Environmental Health, Part A",
title = "Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction",
number = "4",
volume = "84",
doi = "10.1080/15287394.2020.1844827",
pages = "173-182"
}

Platanić Arizanović, L., Nikolić-Kokić, A., Brkljačić, J., Tatalović, N., Miler, M., Oreščanin Dušić, Z., Vidonja Uzelac, T., Nikolić, M., Milošević, V., Blagojević, D., Spasić, S.,& Miljević, Č.. (2021). Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction. in Journal of Toxicology and Environmental Health, Part A
Bellwether Publishing, Ltd.., 84(4), 173-182.
https://doi.org/10.1080/15287394.2020.1844827

Platanić Arizanović L, Nikolić-Kokić A, Brkljačić J, Tatalović N, Miler M, Oreščanin Dušić Z, Vidonja Uzelac T, Nikolić M, Milošević V, Blagojević D, Spasić S, Miljević Č. Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction. in Journal of Toxicology and Environmental Health, Part A. 2021;84(4):173-182.
doi:10.1080/15287394.2020.1844827 .

Platanić Arizanović, Lena, Nikolić-Kokić, Aleksandra, Brkljačić, Jelena, Tatalović, Nikola, Miler, Marko, Oreščanin Dušić, Zorana, Vidonja Uzelac, Teodora, Nikolić, Milan, Milošević, Verica, Blagojević, Duško, Spasić, Snežana, Miljević, Čedo, "Effects of several atypical antipsychotics closapine, sertindole or ziprasidone on hepatic antioxidant enzymes: Possible role in drug-induced liver dysfunction" in Journal of Toxicology and Environmental Health, Part A, 84, no. 4 (2021):173-182,
https://doi.org/10.1080/15287394.2020.1844827 . .

TY  - JOUR
AU  - Vodnik, Vesna V.
AU  - Mojić, Marija
AU  - Stamenović, Una
AU  - Otoničar, Mojca
AU  - Ajdžanović, Vladimir
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Marković, Mirjana M.
AU  - Barudžija, Tanja
AU  - Filipović, Branko
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka 
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4238
AB  - Soy isoflavone genistein (Gen) exerts beneficial effects against prostate cancer cells in vitro and in vivo. However, its use as a chemoprevention/therapeutic agent is largely limited due to its low bioavailability. In this study we synthesized two variants of a new delivery system, genistein–gold nanoparticles conjugates Gen@AuNPs1 and Gen@AuNPs2, by an environmentally friendly method, using a dual role of Gen to reduce Au3+ and stabilize the formed AuNPs, with no additional component. The formation of Gen@AuNPs was confirmed via UV–Vis spectroscopy, FTIR, and Raman spectra measurements. The spherical shape and uniform size of Gen@AuNPs1 and Gen@AuNPs2 (10 ± 2 and 23 ± 3 nm, respectively), were determined by transmission electron microscopy. The nano-conjugates also varied in hydrodynamic diameter (65.0 ± 1.7 and 153.0 ± 2.2 nm) but had similar negative zeta potential (−35.0 ± 2.5 and −37.0 ± 1.6 mV), as measured by dynamic light scattering. The Gen loading was estimated to be 46 and 48%, for Gen@AuNPs1 and Gen@AuNPs2, respectively. The antiproliferative activities of GenAuNPs were confirmed by MTT test in vitro on three malignant prostate carcinoma cell lines (PC3, DU 145, and LNCaP), while selectivity toward malignant phenotype was confirmed using non-cancerous MRC-5 cells. Flow cytometric analysis showed that the inhibition on cell proliferation of more potent Gen@AuNPs1 nano-conjugate is comparable with the effects of free Gen. In conclusion, the obtained results, including physicochemical characterization of newly synthesized AuNPs loaded with Gen, cytotoxicity, and IC50 assessments, indicate their stability and bioactivity as an antioxidant and anti-prostate cancer agent, with low toxicity against human primary cells.
PB  - Elsevier Ltd
T2  - Materials Science and Engineering C
T1  - Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines
VL  - 124
DO  - 10.1016/j.msec.2021.112078
SP  - 112078
ER  - 

@article{
author = "Vodnik, Vesna V. and Mojić, Marija and Stamenović, Una and Otoničar, Mojca and Ajdžanović, Vladimir and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Marković, Mirjana M. and Barudžija, Tanja and Filipović, Branko and Milošević, Verica and Šošić-Jurjević, Branka ",
year = "2021",
abstract = "Soy isoflavone genistein (Gen) exerts beneficial effects against prostate cancer cells in vitro and in vivo. However, its use as a chemoprevention/therapeutic agent is largely limited due to its low bioavailability. In this study we synthesized two variants of a new delivery system, genistein–gold nanoparticles conjugates Gen@AuNPs1 and Gen@AuNPs2, by an environmentally friendly method, using a dual role of Gen to reduce Au3+ and stabilize the formed AuNPs, with no additional component. The formation of Gen@AuNPs was confirmed via UV–Vis spectroscopy, FTIR, and Raman spectra measurements. The spherical shape and uniform size of Gen@AuNPs1 and Gen@AuNPs2 (10 ± 2 and 23 ± 3 nm, respectively), were determined by transmission electron microscopy. The nano-conjugates also varied in hydrodynamic diameter (65.0 ± 1.7 and 153.0 ± 2.2 nm) but had similar negative zeta potential (−35.0 ± 2.5 and −37.0 ± 1.6 mV), as measured by dynamic light scattering. The Gen loading was estimated to be 46 and 48%, for Gen@AuNPs1 and Gen@AuNPs2, respectively. The antiproliferative activities of GenAuNPs were confirmed by MTT test in vitro on three malignant prostate carcinoma cell lines (PC3, DU 145, and LNCaP), while selectivity toward malignant phenotype was confirmed using non-cancerous MRC-5 cells. Flow cytometric analysis showed that the inhibition on cell proliferation of more potent Gen@AuNPs1 nano-conjugate is comparable with the effects of free Gen. In conclusion, the obtained results, including physicochemical characterization of newly synthesized AuNPs loaded with Gen, cytotoxicity, and IC50 assessments, indicate their stability and bioactivity as an antioxidant and anti-prostate cancer agent, with low toxicity against human primary cells.",
publisher = "Elsevier Ltd",
journal = "Materials Science and Engineering C",
title = "Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines",
volume = "124",
doi = "10.1016/j.msec.2021.112078",
pages = "112078"
}

Vodnik, V. V., Mojić, M., Stamenović, U., Otoničar, M., Ajdžanović, V., Maksimović-Ivanić, D., Mijatović, S., Marković, M. M., Barudžija, T., Filipović, B., Milošević, V.,& Šošić-Jurjević, B.. (2021). Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines. in Materials Science and Engineering C
Elsevier Ltd., 124, 112078.
https://doi.org/10.1016/j.msec.2021.112078

Vodnik VV, Mojić M, Stamenović U, Otoničar M, Ajdžanović V, Maksimović-Ivanić D, Mijatović S, Marković MM, Barudžija T, Filipović B, Milošević V, Šošić-Jurjević B. Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines. in Materials Science and Engineering C. 2021;124:112078.
doi:10.1016/j.msec.2021.112078 .

Vodnik, Vesna V., Mojić, Marija, Stamenović, Una, Otoničar, Mojca, Ajdžanović, Vladimir, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Marković, Mirjana M., Barudžija, Tanja, Filipović, Branko, Milošević, Verica, Šošić-Jurjević, Branka , "Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines" in Materials Science and Engineering C, 124 (2021):112078,
https://doi.org/10.1016/j.msec.2021.112078 . .

TY  - JOUR
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Pendovski, Lazo
AU  - Milošević, Verica
PY  - 2021
UR  - http://www.publish.csiro.au/?paper=RD20164
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4153
AB  - Overexposure to glucocorticoids during fetal development alters fetal organ growth and maturation patterns, which can result in adverse programming outcomes in adulthood. The aim of this study was to determine whether exposure to dexamethasone (Dx) during the fetal period programmed ovary development and function in infant (16-day-old) and peripubertal (38-day-old) female offspring. Pregnant Wistar rats were separated into control and Dx-Treated (0.5 mg kg-1) groups and were injected with Dx or an equivalent volume of vehicle on Days 16, 17 and 18 of gestation. Ovaries from 16-and 38-day-old female offspring were prepared for histological and stereological examination. The volume of the ovary and the number of primordial and primary follicles were significantly reduced in prenatally Dx-exposed infant and peripubertal female offspring compared with control offspring. The number of multilaminar follicles was decreased in infant female offspring. In peripubertal females, prenatal exposure to Dx increased the number of multilaminar and large follicles of all classes. Because vaginal opening did not occur up to Day 38 postpartum in the Dx-exposed offspring, the absence of ovulation and corpora lutea is confirmation that the onset of puberty had been delayed. We can conclude that overexposure to glucocorticoids early in life programs ovary development, which may affect fertility in adulthood.
PB  - CSIRO
T2  - Reproduction, Fertility and Development
T1  - Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat
IS  - 3
VL  - 33
DO  - 10.1071/RD20164
SP  - 245
EP  - 255
ER  - 

@article{
author = "Ristić, Nataša and Nestorović, Nataša and Manojlović-Stojanoski, Milica and Trifunović, Svetlana and Ajdžanović, Vladimir and Filipović, Branko and Pendovski, Lazo and Milošević, Verica",
year = "2021",
abstract = "Overexposure to glucocorticoids during fetal development alters fetal organ growth and maturation patterns, which can result in adverse programming outcomes in adulthood. The aim of this study was to determine whether exposure to dexamethasone (Dx) during the fetal period programmed ovary development and function in infant (16-day-old) and peripubertal (38-day-old) female offspring. Pregnant Wistar rats were separated into control and Dx-Treated (0.5 mg kg-1) groups and were injected with Dx or an equivalent volume of vehicle on Days 16, 17 and 18 of gestation. Ovaries from 16-and 38-day-old female offspring were prepared for histological and stereological examination. The volume of the ovary and the number of primordial and primary follicles were significantly reduced in prenatally Dx-exposed infant and peripubertal female offspring compared with control offspring. The number of multilaminar follicles was decreased in infant female offspring. In peripubertal females, prenatal exposure to Dx increased the number of multilaminar and large follicles of all classes. Because vaginal opening did not occur up to Day 38 postpartum in the Dx-exposed offspring, the absence of ovulation and corpora lutea is confirmation that the onset of puberty had been delayed. We can conclude that overexposure to glucocorticoids early in life programs ovary development, which may affect fertility in adulthood.",
publisher = "CSIRO",
journal = "Reproduction, Fertility and Development",
title = "Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat",
number = "3",
volume = "33",
doi = "10.1071/RD20164",
pages = "245-255"
}

Ristić, N., Nestorović, N., Manojlović-Stojanoski, M., Trifunović, S., Ajdžanović, V., Filipović, B., Pendovski, L.,& Milošević, V.. (2021). Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat. in Reproduction, Fertility and Development
CSIRO., 33(3), 245-255.
https://doi.org/10.1071/RD20164

Ristić N, Nestorović N, Manojlović-Stojanoski M, Trifunović S, Ajdžanović V, Filipović B, Pendovski L, Milošević V. Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat. in Reproduction, Fertility and Development. 2021;33(3):245-255.
doi:10.1071/RD20164 .

Ristić, Nataša, Nestorović, Nataša, Manojlović-Stojanoski, Milica, Trifunović, Svetlana, Ajdžanović, Vladimir, Filipović, Branko, Pendovski, Lazo, Milošević, Verica, "Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat" in Reproduction, Fertility and Development, 33, no. 3 (2021):245-255,
https://doi.org/10.1071/RD20164 . .

TY  - JOUR
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Trifunović, Svetlana
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Pendovski, Lazo
AU  - Milošević, Verica
PY  - 2021
UR  - http://www.publish.csiro.au/?paper=RD20164
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4153
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4163
AB  - Overexposure to glucocorticoids during fetal development alters fetal organ growth and maturation patterns, which can result in adverse programming outcomes in adulthood. The aim of this study was to determine whether exposure to dexamethasone (Dx) during the fetal period programmed ovary development and function in infant (16-day-old) and peripubertal (38-day-old) female offspring. Pregnant Wistar rats were separated into control and Dx-Treated (0.5 mg kg-1) groups and were injected with Dx or an equivalent volume of vehicle on Days 16, 17 and 18 of gestation. Ovaries from 16-and 38-day-old female offspring were prepared for histological and stereological examination. The volume of the ovary and the number of primordial and primary follicles were significantly reduced in prenatally Dx-exposed infant and peripubertal female offspring compared with control offspring. The number of multilaminar follicles was decreased in infant female offspring. In peripubertal females, prenatal exposure to Dx increased the number of multilaminar and large follicles of all classes. Because vaginal opening did not occur up to Day 38 postpartum in the Dx-exposed offspring, the absence of ovulation and corpora lutea is confirmation that the onset of puberty had been delayed. We can conclude that overexposure to glucocorticoids early in life programs ovary development, which may affect fertility in adulthood.
PB  - CSIRO
T2  - Reproduction, Fertility and Development
T1  - Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat
IS  - 3
VL  - 33
DO  - 10.1071/RD20164
SP  - 245
EP  - 255
ER  - 

@article{
author = "Ristić, Nataša and Nestorović, Nataša and Manojlović-Stojanoski, Milica and Trifunović, Svetlana and Ajdžanović, Vladimir and Filipović, Branko and Pendovski, Lazo and Milošević, Verica",
year = "2021",
abstract = "Overexposure to glucocorticoids during fetal development alters fetal organ growth and maturation patterns, which can result in adverse programming outcomes in adulthood. The aim of this study was to determine whether exposure to dexamethasone (Dx) during the fetal period programmed ovary development and function in infant (16-day-old) and peripubertal (38-day-old) female offspring. Pregnant Wistar rats were separated into control and Dx-Treated (0.5 mg kg-1) groups and were injected with Dx or an equivalent volume of vehicle on Days 16, 17 and 18 of gestation. Ovaries from 16-and 38-day-old female offspring were prepared for histological and stereological examination. The volume of the ovary and the number of primordial and primary follicles were significantly reduced in prenatally Dx-exposed infant and peripubertal female offspring compared with control offspring. The number of multilaminar follicles was decreased in infant female offspring. In peripubertal females, prenatal exposure to Dx increased the number of multilaminar and large follicles of all classes. Because vaginal opening did not occur up to Day 38 postpartum in the Dx-exposed offspring, the absence of ovulation and corpora lutea is confirmation that the onset of puberty had been delayed. We can conclude that overexposure to glucocorticoids early in life programs ovary development, which may affect fertility in adulthood.",
publisher = "CSIRO",
journal = "Reproduction, Fertility and Development",
title = "Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat",
number = "3",
volume = "33",
doi = "10.1071/RD20164",
pages = "245-255"
}

Ristić, N., Nestorović, N., Manojlović-Stojanoski, M., Trifunović, S., Ajdžanović, V., Filipović, B., Pendovski, L.,& Milošević, V.. (2021). Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat. in Reproduction, Fertility and Development
CSIRO., 33(3), 245-255.
https://doi.org/10.1071/RD20164

Ristić N, Nestorović N, Manojlović-Stojanoski M, Trifunović S, Ajdžanović V, Filipović B, Pendovski L, Milošević V. Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat. in Reproduction, Fertility and Development. 2021;33(3):245-255.
doi:10.1071/RD20164 .

Ristić, Nataša, Nestorović, Nataša, Manojlović-Stojanoski, Milica, Trifunović, Svetlana, Ajdžanović, Vladimir, Filipović, Branko, Pendovski, Lazo, Milošević, Verica, "Prenatal dexamethasone exposure and developmental programming of the ovary of the offspring: a structural study in the rat" in Reproduction, Fertility and Development, 33, no. 3 (2021):245-255,
https://doi.org/10.1071/RD20164 . .

TY  - JOUR
AU  - Ruskovska, Tatjana
AU  - Budić-Leto, Irena
AU  - Corral-Jara, Karla Fabiola
AU  - Ajdžanović, Vladimir
AU  - Arola-Arnal, Anna
AU  - Bravo, Francisca Isabel
AU  - Deligiannidou, Georgia-Eirini
AU  - Havlik, Jaroslav
AU  - Janeva, Milkica
AU  - Kistanova, Elena
AU  - Kontogiorgis, Christos
AU  - Krga, Irena
AU  - Massaro, Marika
AU  - Miler, Marko
AU  - Milošević, Verica
AU  - Morand, Christine
AU  - Scoditti, Egeria
AU  - Suárez, Manuel
AU  - Vauzour, David
AU  - Milenković, Dragan
PY  - 2021
UR  - https://www.mdpi.com/2072-6643/13/7/2326
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4435
AB  - Cardiometabolic disorders are among the leading causes of mortality in the human population. Dietary polyphenols exert beneficial effects on cardiometabolic health in humans. Molecular mechanisms, however, are not completely understood. Aiming to conduct in-depth integrative bioinformatic analyses to elucidate molecular mechanisms underlying the protective effects of polyphenols on cardiometabolic health, we first conducted a systematic literature search to identify human intervention studies with polyphenols that demonstrate improvement of cardiometabolic risk factors in parallel with significant nutrigenomic effects. Applying the predefined inclusion criteria, we identified 58 differentially expressed genes at mRNA level and 5 miRNAs, analyzed in peripheral blood cells with RT-PCR methods. Subsequent integrative bioinformatic analyses demonstrated that polyphenols modulate genes that are mainly involved in the processes such as inflammation, lipid metabolism, and endothelial function. We also identified 37 transcription factors that are involved in the regulation of polyphenol modulated genes, including RELA/NFKB1, STAT1, JUN, or SIRT1. Integrative bioinformatic analysis of mRNA and miRNA-target pathways demonstrated several common enriched pathways that include MAPK signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, focal adhesion, or PPAR signaling pathway. These bioinformatic analyses represent a valuable source of information for the identification of molecular mechanisms underlying the beneficial health effects of polyphenols and potential target genes for future nutrigenetic studies.
PB  - Basel: MDPI
T2  - Nutrients
T1  - Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans-Evidence from Targeted Nutrigenomic Studies.
IS  - 7
VL  - 13
DO  - 10.3390/nu13072326
SP  - 2326
ER  - 

@article{
author = "Ruskovska, Tatjana and Budić-Leto, Irena and Corral-Jara, Karla Fabiola and Ajdžanović, Vladimir and Arola-Arnal, Anna and Bravo, Francisca Isabel and Deligiannidou, Georgia-Eirini and Havlik, Jaroslav and Janeva, Milkica and Kistanova, Elena and Kontogiorgis, Christos and Krga, Irena and Massaro, Marika and Miler, Marko and Milošević, Verica and Morand, Christine and Scoditti, Egeria and Suárez, Manuel and Vauzour, David and Milenković, Dragan",
year = "2021",
abstract = "Cardiometabolic disorders are among the leading causes of mortality in the human population. Dietary polyphenols exert beneficial effects on cardiometabolic health in humans. Molecular mechanisms, however, are not completely understood. Aiming to conduct in-depth integrative bioinformatic analyses to elucidate molecular mechanisms underlying the protective effects of polyphenols on cardiometabolic health, we first conducted a systematic literature search to identify human intervention studies with polyphenols that demonstrate improvement of cardiometabolic risk factors in parallel with significant nutrigenomic effects. Applying the predefined inclusion criteria, we identified 58 differentially expressed genes at mRNA level and 5 miRNAs, analyzed in peripheral blood cells with RT-PCR methods. Subsequent integrative bioinformatic analyses demonstrated that polyphenols modulate genes that are mainly involved in the processes such as inflammation, lipid metabolism, and endothelial function. We also identified 37 transcription factors that are involved in the regulation of polyphenol modulated genes, including RELA/NFKB1, STAT1, JUN, or SIRT1. Integrative bioinformatic analysis of mRNA and miRNA-target pathways demonstrated several common enriched pathways that include MAPK signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, focal adhesion, or PPAR signaling pathway. These bioinformatic analyses represent a valuable source of information for the identification of molecular mechanisms underlying the beneficial health effects of polyphenols and potential target genes for future nutrigenetic studies.",
publisher = "Basel: MDPI",
journal = "Nutrients",
title = "Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans-Evidence from Targeted Nutrigenomic Studies.",
number = "7",
volume = "13",
doi = "10.3390/nu13072326",
pages = "2326"
}

Ruskovska, T., Budić-Leto, I., Corral-Jara, K. F., Ajdžanović, V., Arola-Arnal, A., Bravo, F. I., Deligiannidou, G., Havlik, J., Janeva, M., Kistanova, E., Kontogiorgis, C., Krga, I., Massaro, M., Miler, M., Milošević, V., Morand, C., Scoditti, E., Suárez, M., Vauzour, D.,& Milenković, D.. (2021). Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans-Evidence from Targeted Nutrigenomic Studies.. in Nutrients
Basel: MDPI., 13(7), 2326.
https://doi.org/10.3390/nu13072326

Ruskovska T, Budić-Leto I, Corral-Jara KF, Ajdžanović V, Arola-Arnal A, Bravo FI, Deligiannidou G, Havlik J, Janeva M, Kistanova E, Kontogiorgis C, Krga I, Massaro M, Miler M, Milošević V, Morand C, Scoditti E, Suárez M, Vauzour D, Milenković D. Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans-Evidence from Targeted Nutrigenomic Studies.. in Nutrients. 2021;13(7):2326.
doi:10.3390/nu13072326 .

Ruskovska, Tatjana, Budić-Leto, Irena, Corral-Jara, Karla Fabiola, Ajdžanović, Vladimir, Arola-Arnal, Anna, Bravo, Francisca Isabel, Deligiannidou, Georgia-Eirini, Havlik, Jaroslav, Janeva, Milkica, Kistanova, Elena, Kontogiorgis, Christos, Krga, Irena, Massaro, Marika, Miler, Marko, Milošević, Verica, Morand, Christine, Scoditti, Egeria, Suárez, Manuel, Vauzour, David, Milenković, Dragan, "Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans-Evidence from Targeted Nutrigenomic Studies." in Nutrients, 13, no. 7 (2021):2326,
https://doi.org/10.3390/nu13072326 . .

TY  - JOUR
AU  - Sretenović, Jasmina
AU  - Joksimović Jović, Jovana
AU  - Srejović, Ivan
AU  - Živković, Vladimir
AU  - Mihajlović, Katarina
AU  - Labudović-Borović, Milica
AU  - Trifunović, Svetlana
AU  - Milošević, Verica
AU  - Lazić, Dejan
AU  - Bolevich, Sergey
AU  - Jakovljević, Vladimir
AU  - Milosavljević, Zoran
PY  - 2021
UR  - https://bacandrology.biomedcentral.com/articles/10.1186/s12610-021-00134-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4444
AB  - BACKGROUND During the last decades, the abuse of anabolic androgenic steroids (AASs) has become popular among professional and recreational athletes. The abuse of AASs leads to decreased levels of sex hormones, but the available literature a gives very small pool of data regarding the effects of swimming alone or combined with AASs on testicle tissue. The aim of this study was to investigate the effects of four-week administration of nandrolone decanoate and swimming training alone or in combination on morphometric parameters, androgen receptor (AR) and redox state in testicle tissue. The study included Wistar albino male rats, 10 weeks old, classified into 4 groups: control (T-N-), nandrolone (T-N+), swimming training (T+N-) and swimming training with nandrolone (T+N+). The rats from nandrolone (N+) groups received nandrolone decanoate 20 mg/kg b.w.once per week. The rats from training (T+) groups, swam 1 h/day 5 days/week. The isolated testicles were measured, left testicles were routinely processed for histological analysis, while right testicles were homogenized and prepared for the analysis of the following oxidative stress biomarkers: index of lipid peroxidation (TBARS), nitrites, catalase, superoxide dismutase (SOD), and reduced glutathione (GSH). RESULTS Diameter, as well as cross-section area of seminiferous tubules were decreased by 10 % and 21 % (respectively) in the T-N+ group and by 15% and 41 % (respectively) in the T+N+ group compared to control. Interstitium of the testicles was decreased in all experimental groups. Reduction of immunoreactivity of AR in T-N+ group was 22 %, in T+N+ group was 9 % compared to control. TBARS levels were increased in T+N- and T+N+ groups. Nitrites were decreased in T+N+ group. Catalase activity was increased in all experimental groups. Swimming alone or combined with nandrolone decreased the level of GSH compared to control. SOD activity was decreased in T-N+ and T+N+ groups compared to control. CONCLUSIONS Nandrolone alone or combined with swimming decreased morphometric parameters and amount of AR in testicle tissue. Changes in the redox state indicate reproductive dysfunction. RéSUMé: CONTEXTE: Au cours des dernières décennies, l’abus de stéroïdes androgéniques anabolisants (SAA) est devenu populaire parmi les athlètes professionnels et récréatifs. L’abus des SAA conduit à une diminution des niveaux d’hormones sexuelles, mais la littérature sur les effets de la natation seule ou combinée avec des SAA sur les tissus testiculaires est encore très limité. Le but de cette étude était d’étudier les effets de l’administration de quatre semaines de décanoate de nandrolone et de l’entraînement à la natation seuls ou en combinaison sur les paramètres morphométriques, le récepteur aux androgènes (RA) et l’état redox dans le tissu testiculaire. L’étude a inclus des rats mâles Wistar albinos, âgés de 10 semaines, classés en 4 groupes: contrôle (T-N-), nandrolone (T-N+), entraînement à la natation (T+N-) et entraînement à la natation avec nandrolone (T+N+). Les rats des groupes nandrolone (N+) ont reçu du décanoate de nandrolone 20 mg/kg p.c. une fois par semaine. Les rats des groupes entraînement (T+) nageaient 1 h/jour 5 jours/semaine. Les testicules isolés ont été mesurés, les testicules gauches ont été systématiquement traités pour l’analyse histologique tandis que les testicules droits ont été homogénéisés et préparés pour l’analyse des biomarqueurs de stress oxydatif suivants: indice de peroxydation lipidique (TBARS), nitrites, catalase, superoxyde dismutase (SOD) et glutathion réduit (GSH). RéSULTATS: Le diamètre, ainsi que la section transversale des tubules séminifères ont été réduits de 10 % et 21 % (respectivement) dans le groupe T-N+ et de 15 % et 41 % (respectivement) dans le groupe T+N+ par rapport au groupe témoin. L’interstitium des testicules était diminué dans tous les groupes expérimentaux. La réduction de l’immunoréactivité de RA dans le groupe T-N+ était de 22 %, dans le groupe T+N+ était de 9 % par rapport au groupe témoin. Les niveaux de TBARS ont augmenté dans les groupes T+N- et T+N+. Les nitrites ont diminué dans le groupe T+N+. L’activité de la catalase a été augmentée dans tous les groupes expérimentaux. La natation seule ou combinée à la nandrolone a réduit le niveau de GSH par rapport au contrôle. L’activité de la SOD était diminuée dans les groupes T-N+ et T+N+ par rapport au contrôle. CONCLUSIONS: La nandrolone seule ou combinée à la natation a diminué les paramètres morphométriques et la quantité de RA dans le tissu testiculaire. Les changements de l’état redox indiquent un dysfonctionnement de la reproduction.
PB  - London: BioMed Central Ltd
T2  - Basic and Clinical Andrology
T1  - Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats
IS  - 1
VL  - 31
DO  - 10.1186/s12610-021-00134-8
SP  - 17
ER  - 

@article{
author = "Sretenović, Jasmina and Joksimović Jović, Jovana and Srejović, Ivan and Živković, Vladimir and Mihajlović, Katarina and Labudović-Borović, Milica and Trifunović, Svetlana and Milošević, Verica and Lazić, Dejan and Bolevich, Sergey and Jakovljević, Vladimir and Milosavljević, Zoran",
year = "2021",
abstract = "BACKGROUND During the last decades, the abuse of anabolic androgenic steroids (AASs) has become popular among professional and recreational athletes. The abuse of AASs leads to decreased levels of sex hormones, but the available literature a gives very small pool of data regarding the effects of swimming alone or combined with AASs on testicle tissue. The aim of this study was to investigate the effects of four-week administration of nandrolone decanoate and swimming training alone or in combination on morphometric parameters, androgen receptor (AR) and redox state in testicle tissue. The study included Wistar albino male rats, 10 weeks old, classified into 4 groups: control (T-N-), nandrolone (T-N+), swimming training (T+N-) and swimming training with nandrolone (T+N+). The rats from nandrolone (N+) groups received nandrolone decanoate 20 mg/kg b.w.once per week. The rats from training (T+) groups, swam 1 h/day 5 days/week. The isolated testicles were measured, left testicles were routinely processed for histological analysis, while right testicles were homogenized and prepared for the analysis of the following oxidative stress biomarkers: index of lipid peroxidation (TBARS), nitrites, catalase, superoxide dismutase (SOD), and reduced glutathione (GSH). RESULTS Diameter, as well as cross-section area of seminiferous tubules were decreased by 10 % and 21 % (respectively) in the T-N+ group and by 15% and 41 % (respectively) in the T+N+ group compared to control. Interstitium of the testicles was decreased in all experimental groups. Reduction of immunoreactivity of AR in T-N+ group was 22 %, in T+N+ group was 9 % compared to control. TBARS levels were increased in T+N- and T+N+ groups. Nitrites were decreased in T+N+ group. Catalase activity was increased in all experimental groups. Swimming alone or combined with nandrolone decreased the level of GSH compared to control. SOD activity was decreased in T-N+ and T+N+ groups compared to control. CONCLUSIONS Nandrolone alone or combined with swimming decreased morphometric parameters and amount of AR in testicle tissue. Changes in the redox state indicate reproductive dysfunction. RéSUMé: CONTEXTE: Au cours des dernières décennies, l’abus de stéroïdes androgéniques anabolisants (SAA) est devenu populaire parmi les athlètes professionnels et récréatifs. L’abus des SAA conduit à une diminution des niveaux d’hormones sexuelles, mais la littérature sur les effets de la natation seule ou combinée avec des SAA sur les tissus testiculaires est encore très limité. Le but de cette étude était d’étudier les effets de l’administration de quatre semaines de décanoate de nandrolone et de l’entraînement à la natation seuls ou en combinaison sur les paramètres morphométriques, le récepteur aux androgènes (RA) et l’état redox dans le tissu testiculaire. L’étude a inclus des rats mâles Wistar albinos, âgés de 10 semaines, classés en 4 groupes: contrôle (T-N-), nandrolone (T-N+), entraînement à la natation (T+N-) et entraînement à la natation avec nandrolone (T+N+). Les rats des groupes nandrolone (N+) ont reçu du décanoate de nandrolone 20 mg/kg p.c. une fois par semaine. Les rats des groupes entraînement (T+) nageaient 1 h/jour 5 jours/semaine. Les testicules isolés ont été mesurés, les testicules gauches ont été systématiquement traités pour l’analyse histologique tandis que les testicules droits ont été homogénéisés et préparés pour l’analyse des biomarqueurs de stress oxydatif suivants: indice de peroxydation lipidique (TBARS), nitrites, catalase, superoxyde dismutase (SOD) et glutathion réduit (GSH). RéSULTATS: Le diamètre, ainsi que la section transversale des tubules séminifères ont été réduits de 10 % et 21 % (respectivement) dans le groupe T-N+ et de 15 % et 41 % (respectivement) dans le groupe T+N+ par rapport au groupe témoin. L’interstitium des testicules était diminué dans tous les groupes expérimentaux. La réduction de l’immunoréactivité de RA dans le groupe T-N+ était de 22 %, dans le groupe T+N+ était de 9 % par rapport au groupe témoin. Les niveaux de TBARS ont augmenté dans les groupes T+N- et T+N+. Les nitrites ont diminué dans le groupe T+N+. L’activité de la catalase a été augmentée dans tous les groupes expérimentaux. La natation seule ou combinée à la nandrolone a réduit le niveau de GSH par rapport au contrôle. L’activité de la SOD était diminuée dans les groupes T-N+ et T+N+ par rapport au contrôle. CONCLUSIONS: La nandrolone seule ou combinée à la natation a diminué les paramètres morphométriques et la quantité de RA dans le tissu testiculaire. Les changements de l’état redox indiquent un dysfonctionnement de la reproduction.",
publisher = "London: BioMed Central Ltd",
journal = "Basic and Clinical Andrology",
title = "Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats",
number = "1",
volume = "31",
doi = "10.1186/s12610-021-00134-8",
pages = "17"
}

Sretenović, J., Joksimović Jović, J., Srejović, I., Živković, V., Mihajlović, K., Labudović-Borović, M., Trifunović, S., Milošević, V., Lazić, D., Bolevich, S., Jakovljević, V.,& Milosavljević, Z.. (2021). Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats. in Basic and Clinical Andrology
London: BioMed Central Ltd., 31(1), 17.
https://doi.org/10.1186/s12610-021-00134-8

Sretenović J, Joksimović Jović J, Srejović I, Živković V, Mihajlović K, Labudović-Borović M, Trifunović S, Milošević V, Lazić D, Bolevich S, Jakovljević V, Milosavljević Z. Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats. in Basic and Clinical Andrology. 2021;31(1):17.
doi:10.1186/s12610-021-00134-8 .

Sretenović, Jasmina, Joksimović Jović, Jovana, Srejović, Ivan, Živković, Vladimir, Mihajlović, Katarina, Labudović-Borović, Milica, Trifunović, Svetlana, Milošević, Verica, Lazić, Dejan, Bolevich, Sergey, Jakovljević, Vladimir, Milosavljević, Zoran, "Morphometric analysis and redox state of the testicles in nandrolone decanoate and swimming treated adult male rats" in Basic and Clinical Andrology, 31, no. 1 (2021):17,
https://doi.org/10.1186/s12610-021-00134-8 . .

TY  - JOUR
AU  - Joksimović Jović, Jovana
AU  - Jović, Nikola
AU  - Sretenović, Jasmina
AU  - Živković, Vladimir
AU  - Nikolić, Maja
AU  - Rudić, Jovan
AU  - Milošević, Verica
AU  - Ristić, Nataša
AU  - Andrić, Kristina
AU  - Dimkić Tomić, Tijana
AU  - Miličić, Biljana
AU  - Jakovljević, Vladimir
PY  - 2021
UR  - https://rep.bioscientifica.com/view/journals/rep/163/1/REP-21-0219.xml
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4771
AB  - Numerous evidence implies complex interrelations between polycystic ovary syndrome (PCOS) and hypertension (HT) in reproductive-age women. In this study, we aimed to investigate the potential strain differences in ovarian morphology, hemodynamic, and biochemical characteristics in an androgen-induced PCOS rat model. A total of 24 rats of 3 weeks old (12 Wistar Kyoto - WK and 12 spontaneously hypertensive rats - SHR) were divided into four groups: WK, WK PCOS, SHR, and SHR PCOS. PCOS was induced by daily s.c. injections of testosterone enanthate (1 mg/100 g body weight) administered for 5 weeks. PCOS induction led to estrus cyclicity cessation, cystic ovarian appearance, and sex hormones disturbances in both strains. The morphometric parameters in ovaries were altered in a manner of PCOS-related changes in both strains (higher number in preantral, atretic, and cystic follicles). Ultrasonographically, a significant decrease in ovarian volume (OV) was registered in PCOS groups but also in SHR compared to WK rats. All blood pressure parameters were higher in SHR compared to WK. PCOS modeling increased systolic, mean arterial, and pulse pressure in WK strain, while in SHR, only mean arterial and pulse pressure were higher. Alterations in oxidative stress parameters could provide a molecular basis for PCOS-related changes: in PCOS groups, thiobarbituric acid reactive substance and superoxide anion radical levels were higher in both strains, while superoxide dismutase and glutathione were significantly lowered.
PB  - Society For Reproduction And Fertility
T2  - Reproduction (Cambridge, England)
T1  - Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.
IS  - 1
VL  - 163
DO  - 10.1530/REP-21-0219
SP  - 11
EP  - 21
ER  - 

@article{
author = "Joksimović Jović, Jovana and Jović, Nikola and Sretenović, Jasmina and Živković, Vladimir and Nikolić, Maja and Rudić, Jovan and Milošević, Verica and Ristić, Nataša and Andrić, Kristina and Dimkić Tomić, Tijana and Miličić, Biljana and Jakovljević, Vladimir",
year = "2021",
abstract = "Numerous evidence implies complex interrelations between polycystic ovary syndrome (PCOS) and hypertension (HT) in reproductive-age women. In this study, we aimed to investigate the potential strain differences in ovarian morphology, hemodynamic, and biochemical characteristics in an androgen-induced PCOS rat model. A total of 24 rats of 3 weeks old (12 Wistar Kyoto - WK and 12 spontaneously hypertensive rats - SHR) were divided into four groups: WK, WK PCOS, SHR, and SHR PCOS. PCOS was induced by daily s.c. injections of testosterone enanthate (1 mg/100 g body weight) administered for 5 weeks. PCOS induction led to estrus cyclicity cessation, cystic ovarian appearance, and sex hormones disturbances in both strains. The morphometric parameters in ovaries were altered in a manner of PCOS-related changes in both strains (higher number in preantral, atretic, and cystic follicles). Ultrasonographically, a significant decrease in ovarian volume (OV) was registered in PCOS groups but also in SHR compared to WK rats. All blood pressure parameters were higher in SHR compared to WK. PCOS modeling increased systolic, mean arterial, and pulse pressure in WK strain, while in SHR, only mean arterial and pulse pressure were higher. Alterations in oxidative stress parameters could provide a molecular basis for PCOS-related changes: in PCOS groups, thiobarbituric acid reactive substance and superoxide anion radical levels were higher in both strains, while superoxide dismutase and glutathione were significantly lowered.",
publisher = "Society For Reproduction And Fertility",
journal = "Reproduction (Cambridge, England)",
title = "Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.",
number = "1",
volume = "163",
doi = "10.1530/REP-21-0219",
pages = "11-21"
}

Joksimović Jović, J., Jović, N., Sretenović, J., Živković, V., Nikolić, M., Rudić, J., Milošević, V., Ristić, N., Andrić, K., Dimkić Tomić, T., Miličić, B.,& Jakovljević, V.. (2021). Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.. in Reproduction (Cambridge, England)
Society For Reproduction And Fertility., 163(1), 11-21.
https://doi.org/10.1530/REP-21-0219

Joksimović Jović J, Jović N, Sretenović J, Živković V, Nikolić M, Rudić J, Milošević V, Ristić N, Andrić K, Dimkić Tomić T, Miličić B, Jakovljević V. Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.. in Reproduction (Cambridge, England). 2021;163(1):11-21.
doi:10.1530/REP-21-0219 .

Joksimović Jović, Jovana, Jović, Nikola, Sretenović, Jasmina, Živković, Vladimir, Nikolić, Maja, Rudić, Jovan, Milošević, Verica, Ristić, Nataša, Andrić, Kristina, Dimkić Tomić, Tijana, Miličić, Biljana, Jakovljević, Vladimir, "Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress." in Reproduction (Cambridge, England), 163, no. 1 (2021):11-21,
https://doi.org/10.1530/REP-21-0219 . .

TY  - JOUR
AU  - Popovska-Perčinić, Florina
AU  - Manojlović-Stojanoski, Milica
AU  - Pendovski, Lazo
AU  - Dinevska Kjovkarovska, Suzana
AU  - Miova, Biljana
AU  - Grubin, Jasmina
AU  - Milošević, Verica
AU  - Ajdžanović, Vladimir
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/123456789/3867
AB  - Objective: As a consequence of global warming, the increase in the average annual temperature is observed, while the living organisms actively adapt to these changes. High environmental temperature initiates numerous physiological, autonomic, and behavioral responses, and activates the stress response. Thus, the aim of the study was to investigate effect of a moderate increase in ambient temperature on the activity of the hypothalamic-pituitary-adrenocortical (HPA) axis by determining histological changes in adrenal glands and hormonal levels in adult male rats.

Materials and methods: In this experimental study, the morpho-functional state of adrenal glands was estimated by stereological evaluation of parameters, including the adrenal volume, adrenocortical cell/nuclear size and number, and the volume density of vascular tissues after four days of exposure to a moderate increase in ambient temperature of 35 ± 1˚C. Novelli histochemical and vascular endothelial growth factor (VEGF) immunohistochemical staining provided insight into the adrenal gland vascular network. Additionally, the adrenal levels of aldosterone, corticosterone, and pituitary adrenocorticotropic hormone (ACTH) were determined as crucial indicators of the hypothalamic-pituitaryadrenocortical (HPA) axis activity.

Results: Prolonged exposure to a moderate increase in ambient temperature for four days resulted in a significant increase in ACTH level up to 24%, which altered adrenal glands both structurally and functionally. The adrenocortical volume and number of cells in all cortical zones were markedly increased (P<0.05). A statistically significant increase was shown in the level of aldosterone (16%) and corticosterone (25%) in serum levels of individuals.

Conclusion: Increased activity of the HPA axis reflects the response to a moderate increase in ambient temperature during four days, showing the capacity of the HPA axis to adapt the organism to daily temperature changes.
PB  - Royan Institute
T2  - Cell Journal
T1  - A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats
IS  - 4
VL  - 22
DO  - 10.22074/cellj.2021.6827
SP  - 415
EP  - 424
ER  - 

@article{
author = "Popovska-Perčinić, Florina and Manojlović-Stojanoski, Milica and Pendovski, Lazo and Dinevska Kjovkarovska, Suzana and Miova, Biljana and Grubin, Jasmina and Milošević, Verica and Ajdžanović, Vladimir",
year = "2021",
abstract = "Objective: As a consequence of global warming, the increase in the average annual temperature is observed, while the living organisms actively adapt to these changes. High environmental temperature initiates numerous physiological, autonomic, and behavioral responses, and activates the stress response. Thus, the aim of the study was to investigate effect of a moderate increase in ambient temperature on the activity of the hypothalamic-pituitary-adrenocortical (HPA) axis by determining histological changes in adrenal glands and hormonal levels in adult male rats.

Materials and methods: In this experimental study, the morpho-functional state of adrenal glands was estimated by stereological evaluation of parameters, including the adrenal volume, adrenocortical cell/nuclear size and number, and the volume density of vascular tissues after four days of exposure to a moderate increase in ambient temperature of 35 ± 1˚C. Novelli histochemical and vascular endothelial growth factor (VEGF) immunohistochemical staining provided insight into the adrenal gland vascular network. Additionally, the adrenal levels of aldosterone, corticosterone, and pituitary adrenocorticotropic hormone (ACTH) were determined as crucial indicators of the hypothalamic-pituitaryadrenocortical (HPA) axis activity.

Results: Prolonged exposure to a moderate increase in ambient temperature for four days resulted in a significant increase in ACTH level up to 24%, which altered adrenal glands both structurally and functionally. The adrenocortical volume and number of cells in all cortical zones were markedly increased (P<0.05). A statistically significant increase was shown in the level of aldosterone (16%) and corticosterone (25%) in serum levels of individuals.

Conclusion: Increased activity of the HPA axis reflects the response to a moderate increase in ambient temperature during four days, showing the capacity of the HPA axis to adapt the organism to daily temperature changes.",
publisher = "Royan Institute",
journal = "Cell Journal",
title = "A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats",
number = "4",
volume = "22",
doi = "10.22074/cellj.2021.6827",
pages = "415-424"
}

Popovska-Perčinić, F., Manojlović-Stojanoski, M., Pendovski, L., Dinevska Kjovkarovska, S., Miova, B., Grubin, J., Milošević, V.,& Ajdžanović, V.. (2021). A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats. in Cell Journal
Royan Institute., 22(4), 415-424.
https://doi.org/10.22074/cellj.2021.6827

Popovska-Perčinić F, Manojlović-Stojanoski M, Pendovski L, Dinevska Kjovkarovska S, Miova B, Grubin J, Milošević V, Ajdžanović V. A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats. in Cell Journal. 2021;22(4):415-424.
doi:10.22074/cellj.2021.6827 .

Popovska-Perčinić, Florina, Manojlović-Stojanoski, Milica, Pendovski, Lazo, Dinevska Kjovkarovska, Suzana, Miova, Biljana, Grubin, Jasmina, Milošević, Verica, Ajdžanović, Vladimir, "A moderate increase in ambient temperature influences the structure and hormonal secretion of adrenal glands in rats" in Cell Journal, 22, no. 4 (2021):415-424,
https://doi.org/10.22074/cellj.2021.6827 . .

TY  - JOUR
AU  - Rauš Balind, Snežana
AU  - Manojlović-Stojanoski, Milica
AU  - Šošić-Jurjević, Branka 
AU  - Selaković, Vesna
AU  - Milošević, Verica
AU  - Petković, Branka
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/bem.22237
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3570
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3764
AB  - The neuroendocrine system can be modulated by a magnetic field and cerebral ischemia as external and internal stressors, respectively. This study deals with the separate or combined effects of an extremely low frequency (ELF) magnetic field (50 Hz, average magnetic field of 0.5 mT) for 7 days and global cerebral ischemia for 10 min on the morpho-functional features of pituitary adrenocorticotrophic (ACTH) and thyrotrophic (TSH) cells in 3-month-old gerbils. To determine the immediate and delayed effects of the applied stressors, measurements were made on the 7th and 14th days after the onset of the experiment. The ELF magnetic field and 10-min global cerebral ischemia, separately and particularly in combination, decreased (P < 0.05) the volume density of ACTH cells, while only in combination were intracellular ACTH content and plasma ACTH concentration increased (P < 0.05) on day 7. The ELF magnetic field elevated serum TSH concentration on day 7 and intracellular TSHβ content on day 14 (P < 0.05). Also, 10-min global cerebral ischemia alone increased serum TSH concentration (P < 0.05), while in combination with the ELF magnetic field it elevated (P < 0.05) intracellular TSHβ content on day 14. In conclusion, an ELF magnetic field and/or 10-min global cerebral ischemia can induce immediate and delayed stimulation of ACTH and TSH synthesis and secretion.
PB  - John Wiley & Sons, Ltd
T2  - Bioelectromagnetics
T1  - An Extremely Low Frequency Magnetic Field and Global Cerebral Ischemia Affect Pituitary ACTH and TSH Cells in Gerbils
IS  - 2
VL  - 41
DO  - 10.1002/bem.22237
SP  - 91
EP  - 103
ER  - 

@article{
author = "Rauš Balind, Snežana and Manojlović-Stojanoski, Milica and Šošić-Jurjević, Branka  and Selaković, Vesna and Milošević, Verica and Petković, Branka",
year = "2020",
abstract = "The neuroendocrine system can be modulated by a magnetic field and cerebral ischemia as external and internal stressors, respectively. This study deals with the separate or combined effects of an extremely low frequency (ELF) magnetic field (50 Hz, average magnetic field of 0.5 mT) for 7 days and global cerebral ischemia for 10 min on the morpho-functional features of pituitary adrenocorticotrophic (ACTH) and thyrotrophic (TSH) cells in 3-month-old gerbils. To determine the immediate and delayed effects of the applied stressors, measurements were made on the 7th and 14th days after the onset of the experiment. The ELF magnetic field and 10-min global cerebral ischemia, separately and particularly in combination, decreased (P < 0.05) the volume density of ACTH cells, while only in combination were intracellular ACTH content and plasma ACTH concentration increased (P < 0.05) on day 7. The ELF magnetic field elevated serum TSH concentration on day 7 and intracellular TSHβ content on day 14 (P < 0.05). Also, 10-min global cerebral ischemia alone increased serum TSH concentration (P < 0.05), while in combination with the ELF magnetic field it elevated (P < 0.05) intracellular TSHβ content on day 14. In conclusion, an ELF magnetic field and/or 10-min global cerebral ischemia can induce immediate and delayed stimulation of ACTH and TSH synthesis and secretion.",
publisher = "John Wiley & Sons, Ltd",
journal = "Bioelectromagnetics",
title = "An Extremely Low Frequency Magnetic Field and Global Cerebral Ischemia Affect Pituitary ACTH and TSH Cells in Gerbils",
number = "2",
volume = "41",
doi = "10.1002/bem.22237",
pages = "91-103"
}

Rauš Balind, S., Manojlović-Stojanoski, M., Šošić-Jurjević, B., Selaković, V., Milošević, V.,& Petković, B.. (2020). An Extremely Low Frequency Magnetic Field and Global Cerebral Ischemia Affect Pituitary ACTH and TSH Cells in Gerbils. in Bioelectromagnetics
John Wiley & Sons, Ltd., 41(2), 91-103.
https://doi.org/10.1002/bem.22237

Rauš Balind S, Manojlović-Stojanoski M, Šošić-Jurjević B, Selaković V, Milošević V, Petković B. An Extremely Low Frequency Magnetic Field and Global Cerebral Ischemia Affect Pituitary ACTH and TSH Cells in Gerbils. in Bioelectromagnetics. 2020;41(2):91-103.
doi:10.1002/bem.22237 .

Rauš Balind, Snežana, Manojlović-Stojanoski, Milica, Šošić-Jurjević, Branka , Selaković, Vesna, Milošević, Verica, Petković, Branka, "An Extremely Low Frequency Magnetic Field and Global Cerebral Ischemia Affect Pituitary ACTH and TSH Cells in Gerbils" in Bioelectromagnetics, 41, no. 2 (2020):91-103,
https://doi.org/10.1002/bem.22237 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Popovska-Perčinić, Florina
AU  - Milošević, Verica
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32120001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3627
AB  - INTRODUCTION AND AIM Daidzein application may represent an effective and less harmful alternative to indicated, classical estrogenization of ageing men. The aim of this study was to perform structural and hormonal analysis of the adrenal cortex, after estradiol or daidzein supplementation in a rat model of the andropause. MATERIAL AND METHODS Middle-aged Wistar rats were divided into sham operated (SO; n = 8), orchidectomized (Orx; n = 8), estradiol treated orchidectomized (Orx + E; n = 8) and daidzein treated orchidectomized (Orx + D; n = 8) groups. Estradiol (0.625 mg/kg b.m./day) or daidzein (30 mg/kg b.m./day) were administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis. RESULTS Both estradiol and daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei, but decreased circulating aldosterone levels. Estradiol markedly increased volumes of the zona fasciculata cell and nuclei in parallel with significant decrease of the adrenal tissue level of corticosterone, while daidzein significantly decreased both the adrenal and circulating levels of corticosterone. Serum DHEA level and volumes of the zona reticularis cell and nuclei significantly increased upon estradiol treatment, whereas daidzein even stronger increased the circulating level of DHEA. Shunting of the corticosteroidogenesis pathways towards adrenal androgens production, after the treatments, corresponded to the ultrastructural findings and zonal capillary network rearrangements. CONCLUSIONS Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of ageing-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.
T2  - Annals of Anatomy
T1  - The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study.
VL  - 230
DO  - 10.1016/j.aanat.2020.151487
SP  - 151487
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Živanović, Jasmina and Filipović, Branko and Šošić-Jurjević, Branka  and Popovska-Perčinić, Florina and Milošević, Verica",
year = "2020",
abstract = "INTRODUCTION AND AIM Daidzein application may represent an effective and less harmful alternative to indicated, classical estrogenization of ageing men. The aim of this study was to perform structural and hormonal analysis of the adrenal cortex, after estradiol or daidzein supplementation in a rat model of the andropause. MATERIAL AND METHODS Middle-aged Wistar rats were divided into sham operated (SO; n = 8), orchidectomized (Orx; n = 8), estradiol treated orchidectomized (Orx + E; n = 8) and daidzein treated orchidectomized (Orx + D; n = 8) groups. Estradiol (0.625 mg/kg b.m./day) or daidzein (30 mg/kg b.m./day) were administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis. RESULTS Both estradiol and daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei, but decreased circulating aldosterone levels. Estradiol markedly increased volumes of the zona fasciculata cell and nuclei in parallel with significant decrease of the adrenal tissue level of corticosterone, while daidzein significantly decreased both the adrenal and circulating levels of corticosterone. Serum DHEA level and volumes of the zona reticularis cell and nuclei significantly increased upon estradiol treatment, whereas daidzein even stronger increased the circulating level of DHEA. Shunting of the corticosteroidogenesis pathways towards adrenal androgens production, after the treatments, corresponded to the ultrastructural findings and zonal capillary network rearrangements. CONCLUSIONS Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of ageing-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.",
journal = "Annals of Anatomy",
title = "The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study.",
volume = "230",
doi = "10.1016/j.aanat.2020.151487",
pages = "151487"
}

Ajdžanović, V., Miler, M., Živanović, J., Filipović, B., Šošić-Jurjević, B., Popovska-Perčinić, F.,& Milošević, V.. (2020). The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study.. in Annals of Anatomy, 230, 151487.
https://doi.org/10.1016/j.aanat.2020.151487

Ajdžanović V, Miler M, Živanović J, Filipović B, Šošić-Jurjević B, Popovska-Perčinić F, Milošević V. The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study.. in Annals of Anatomy. 2020;230:151487.
doi:10.1016/j.aanat.2020.151487 .

Ajdžanović, Vladimir, Miler, Marko, Živanović, Jasmina, Filipović, Branko, Šošić-Jurjević, Branka , Popovska-Perčinić, Florina, Milošević, Verica, "The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study." in Annals of Anatomy, 230 (2020):151487,
https://doi.org/10.1016/j.aanat.2020.151487 . .