TY  - CONF
AU  - Davidović, Slobodan
AU  - Marinković, Saša
AU  - Hribšek, Irena
AU  - Popić, Vuk
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6416
AB  - Genetic diversity is highlighted by IUCN as one of the three pillars of biodiversity that need to be protected together with species and ecosystem diversity. Thus, before any conservation measures of reintroduction/restocking are undertaken, especially in the regions where autochthonous populations exist, it is necessary to evaluate the genetic diversity of both the autochthonous and introducing populations. If there are no autochthonous populations, the nearest neighbouring populations are used as donors and only if neighbouring populations are non-existent distant populations could be used. In this way, the local adaptations and genetic variation specific to the region will be preserved.
In the Balkan Peninsula, the stable and autochthonous population of Griffon vulture persists and it should be protected as such. The genetic data based on microsatellites demonstrated the existence of two genetic clusters one of which is specific for the Balkan and Iberian Peninsula. The study of mitochondrial DNA diversity demonstrated the existence of private haplotypes unique for both peninsulas. Long-time monitoring of named populations demonstrated different migration patterns. Birds from the Balkans migrate almost exclusively to the Middle East while the Iberian birds migrate to northwest Africa, which is the reason why they rarely come into contact. Natal philopatric behaviour specific to the species dictates that the unique genetic fingerprint will remain fixed in the region of their origin. Thus, in nature, gene flow between named populations is low, or insignificant. In addition, somewhat different climate conditions between Iberian and Balkan peninsula could also drive genetic differentiation due to the local adaptations.
The detected level of genetic differentiation between these regions suggests that different conservation approaches are needed to preserve specific and unique genetic diversity and that the current program of restocking the vulture population of the Balkan Peninsula with the birds from the Iberian Peninsula needs to be re-evaluated.
PB  - Caceres: Vulture Conservation Foundation
C3  - Abstract book: European Vulture Conference: Vultures 2023; 2023 Nov 14-17; Caceres, Spain
T1  - Using genetics for creating proper conservation strategies for protected species – the case of Griffon Vulture (Gyps fulvus)
SP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6416
ER  - 

@conference{
author = "Davidović, Slobodan and Marinković, Saša and Hribšek, Irena and Popić, Vuk and Erić, Pavle and Patenković, Aleksandra and Tanasković, Marija",
year = "2023",
abstract = "Genetic diversity is highlighted by IUCN as one of the three pillars of biodiversity that need to be protected together with species and ecosystem diversity. Thus, before any conservation measures of reintroduction/restocking are undertaken, especially in the regions where autochthonous populations exist, it is necessary to evaluate the genetic diversity of both the autochthonous and introducing populations. If there are no autochthonous populations, the nearest neighbouring populations are used as donors and only if neighbouring populations are non-existent distant populations could be used. In this way, the local adaptations and genetic variation specific to the region will be preserved.
In the Balkan Peninsula, the stable and autochthonous population of Griffon vulture persists and it should be protected as such. The genetic data based on microsatellites demonstrated the existence of two genetic clusters one of which is specific for the Balkan and Iberian Peninsula. The study of mitochondrial DNA diversity demonstrated the existence of private haplotypes unique for both peninsulas. Long-time monitoring of named populations demonstrated different migration patterns. Birds from the Balkans migrate almost exclusively to the Middle East while the Iberian birds migrate to northwest Africa, which is the reason why they rarely come into contact. Natal philopatric behaviour specific to the species dictates that the unique genetic fingerprint will remain fixed in the region of their origin. Thus, in nature, gene flow between named populations is low, or insignificant. In addition, somewhat different climate conditions between Iberian and Balkan peninsula could also drive genetic differentiation due to the local adaptations.
The detected level of genetic differentiation between these regions suggests that different conservation approaches are needed to preserve specific and unique genetic diversity and that the current program of restocking the vulture population of the Balkan Peninsula with the birds from the Iberian Peninsula needs to be re-evaluated.",
publisher = "Caceres: Vulture Conservation Foundation",
journal = "Abstract book: European Vulture Conference: Vultures 2023; 2023 Nov 14-17; Caceres, Spain",
title = "Using genetics for creating proper conservation strategies for protected species – the case of Griffon Vulture (Gyps fulvus)",
pages = "54",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6416"
}

Davidović, S., Marinković, S., Hribšek, I., Popić, V., Erić, P., Patenković, A.,& Tanasković, M.. (2023). Using genetics for creating proper conservation strategies for protected species – the case of Griffon Vulture (Gyps fulvus). in Abstract book: European Vulture Conference: Vultures 2023; 2023 Nov 14-17; Caceres, Spain
Caceres: Vulture Conservation Foundation., 54.
https://hdl.handle.net/21.15107/rcub_ibiss_6416

Davidović S, Marinković S, Hribšek I, Popić V, Erić P, Patenković A, Tanasković M. Using genetics for creating proper conservation strategies for protected species – the case of Griffon Vulture (Gyps fulvus). in Abstract book: European Vulture Conference: Vultures 2023; 2023 Nov 14-17; Caceres, Spain. 2023;:54.
https://hdl.handle.net/21.15107/rcub_ibiss_6416 .

Davidović, Slobodan, Marinković, Saša, Hribšek, Irena, Popić, Vuk, Erić, Pavle, Patenković, Aleksandra, Tanasković, Marija, "Using genetics for creating proper conservation strategies for protected species – the case of Griffon Vulture (Gyps fulvus)" in Abstract book: European Vulture Conference: Vultures 2023; 2023 Nov 14-17; Caceres, Spain (2023):54,
https://hdl.handle.net/21.15107/rcub_ibiss_6416 .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Erić, Pavle
AU  - Tanasković, Marija
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6388
AB  - Honey bees (Apis mellifera) and bumblebees (Bombus terrestris) are pollinators that cover large areas during their foraging activities. As a result, they encounter various environments and pollutants, making them an ideal choice as bioindicators for environmental pollution. The town of Bor in eastern Serbia houses the largest mine for copper, gold, and silver extraction, and is crucial to regularly monitor the environmental impact of mining activities.
The presence and concentration of heavy metals (Fe, Cu, Zn, Cd, Li, Pb, Au, Ni) were measured using the ICP OES method for samples of both species at six locations in the basin of the Bor mining facility and one in the remote region. Results indicate that both species accumulate heavy metals in their bodies during foraging activities in similar manner. Elevated  concentrations of Fe, Zn, Cd and Pb were detected in both species in the same localities while Cu, Li and Ni were variable depending on the locality. Interestingly the Cu concentration in A. mellifera was less variable than in B. terrestris indicating different accumulation pattern, possibly species specific.
Both pollinator species serve as potential bioindicators for environmental pollution in the area, reflecting ecosystem health and heavy metal infiltration in the food chain.
PB  - Toronto, Canada: York University
C3  - BeeCon 2023; 2023 Oct 12-13; Toronto, Canada
T1  - Two different pollinator species Apis mellifera and Bombus terrestris as indicators of heavy metal pollution near the mining complex in Bor, Serbia
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6388
ER  - 

@conference{
author = "Davidović, Slobodan and Patenković, Aleksandra and Erić, Katarina and Erić, Pavle and Tanasković, Marija",
year = "2023",
abstract = "Honey bees (Apis mellifera) and bumblebees (Bombus terrestris) are pollinators that cover large areas during their foraging activities. As a result, they encounter various environments and pollutants, making them an ideal choice as bioindicators for environmental pollution. The town of Bor in eastern Serbia houses the largest mine for copper, gold, and silver extraction, and is crucial to regularly monitor the environmental impact of mining activities.
The presence and concentration of heavy metals (Fe, Cu, Zn, Cd, Li, Pb, Au, Ni) were measured using the ICP OES method for samples of both species at six locations in the basin of the Bor mining facility and one in the remote region. Results indicate that both species accumulate heavy metals in their bodies during foraging activities in similar manner. Elevated  concentrations of Fe, Zn, Cd and Pb were detected in both species in the same localities while Cu, Li and Ni were variable depending on the locality. Interestingly the Cu concentration in A. mellifera was less variable than in B. terrestris indicating different accumulation pattern, possibly species specific.
Both pollinator species serve as potential bioindicators for environmental pollution in the area, reflecting ecosystem health and heavy metal infiltration in the food chain.",
publisher = "Toronto, Canada: York University",
journal = "BeeCon 2023; 2023 Oct 12-13; Toronto, Canada",
title = "Two different pollinator species Apis mellifera and Bombus terrestris as indicators of heavy metal pollution near the mining complex in Bor, Serbia",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6388"
}

Davidović, S., Patenković, A., Erić, K., Erić, P.,& Tanasković, M.. (2023). Two different pollinator species Apis mellifera and Bombus terrestris as indicators of heavy metal pollution near the mining complex in Bor, Serbia. in BeeCon 2023; 2023 Oct 12-13; Toronto, Canada
Toronto, Canada: York University., 8.
https://hdl.handle.net/21.15107/rcub_ibiss_6388

Davidović S, Patenković A, Erić K, Erić P, Tanasković M. Two different pollinator species Apis mellifera and Bombus terrestris as indicators of heavy metal pollution near the mining complex in Bor, Serbia. in BeeCon 2023; 2023 Oct 12-13; Toronto, Canada. 2023;:8.
https://hdl.handle.net/21.15107/rcub_ibiss_6388 .

Davidović, Slobodan, Patenković, Aleksandra, Erić, Katarina, Erić, Pavle, Tanasković, Marija, "Two different pollinator species Apis mellifera and Bombus terrestris as indicators of heavy metal pollution near the mining complex in Bor, Serbia" in BeeCon 2023; 2023 Oct 12-13; Toronto, Canada (2023):8,
https://hdl.handle.net/21.15107/rcub_ibiss_6388 .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Stanković, Milica
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5868
AB  - White-tailed eagle is the biggest bird of prey in Central and Southeast Europe. In Serbia it inhabits the Vojvodina province and the valleys of Danube, Sava, Tisa and Tamiš. Anthropogenic pressure on its habitats in Europe caused a decline in its numbers, but due to the strict laws protecting both species and its habitats, birds’ numbers are now steady and increasing. In Serbia, as a strictly protected species it is a subject of different conservation programs. The available genetic data for this population are scarce and it is necessary to assess its genetic diversity to improve the existing conservation efforts. ND1 and Cytb mitochondrial genes can be used to estimate the populations’ adaptation to different environmental conditions and their variability can potentially be used to evaluate differentiation between populations.
To assess the genetic diversity of White-tailed eagle in Serbia we used mitochondrial ND1 and Cytb nucleotide sequences from 40 unrelated birds collected in nests. ND1 and Cytb nucleotide sequences variability was evaluated using standard parameters of genetic diversity (PGD). Acquired values were compared with the available data for the variability of the D-loop region which showed that combined ND1/Cytb nucleotide sequences PGD provide comparable results. Using publicly available sequences we reconstructed haplotype networks for ND1, Cytb, ND1/Cytb and D-loop which further showed the applicability of ND1/Cytb in population genetics analyses. Phylogeny reconstructed using combined ND1/Cytb sequences identified two branches in Serbian white-tailed eagles. Although the majority of substitutions were nonsynonymous, no selective pressure was detected.
Our data suggest that combined ND1/Cytb sequence variability provides sufficient information to be used for population comparison, population differentiation analyses and phylogeny reconstruction, but also gives a tool to potentially identify adaptations to different environmental conditions.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
C3  - Book of Abstracts: 4th Belgrade Bioinformatics Conference: BELBI2023; 2023 Jun 1-23; Belgrade, Serbia
T1  - Evaluating ND1 and Cytb mitochondrial genes as markers for diversity analysis of protected White-tailed eagle species from Serbia
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5868
ER  - 

@conference{
editor = "Morić, Ivana, Đorđević, Valentina",
author = "Davidović, Slobodan and Stanković, Milica and Erić, Pavle and Erić, Katarina and Patenković, Aleksandra and Tanasković, Marija",
year = "2023",
abstract = "White-tailed eagle is the biggest bird of prey in Central and Southeast Europe. In Serbia it inhabits the Vojvodina province and the valleys of Danube, Sava, Tisa and Tamiš. Anthropogenic pressure on its habitats in Europe caused a decline in its numbers, but due to the strict laws protecting both species and its habitats, birds’ numbers are now steady and increasing. In Serbia, as a strictly protected species it is a subject of different conservation programs. The available genetic data for this population are scarce and it is necessary to assess its genetic diversity to improve the existing conservation efforts. ND1 and Cytb mitochondrial genes can be used to estimate the populations’ adaptation to different environmental conditions and their variability can potentially be used to evaluate differentiation between populations.
To assess the genetic diversity of White-tailed eagle in Serbia we used mitochondrial ND1 and Cytb nucleotide sequences from 40 unrelated birds collected in nests. ND1 and Cytb nucleotide sequences variability was evaluated using standard parameters of genetic diversity (PGD). Acquired values were compared with the available data for the variability of the D-loop region which showed that combined ND1/Cytb nucleotide sequences PGD provide comparable results. Using publicly available sequences we reconstructed haplotype networks for ND1, Cytb, ND1/Cytb and D-loop which further showed the applicability of ND1/Cytb in population genetics analyses. Phylogeny reconstructed using combined ND1/Cytb sequences identified two branches in Serbian white-tailed eagles. Although the majority of substitutions were nonsynonymous, no selective pressure was detected.
Our data suggest that combined ND1/Cytb sequence variability provides sufficient information to be used for population comparison, population differentiation analyses and phylogeny reconstruction, but also gives a tool to potentially identify adaptations to different environmental conditions.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Book of Abstracts: 4th Belgrade Bioinformatics Conference: BELBI2023; 2023 Jun 1-23; Belgrade, Serbia",
title = "Evaluating ND1 and Cytb mitochondrial genes as markers for diversity analysis of protected White-tailed eagle species from Serbia",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5868"
}

Morić, I., Đorđević, V., Davidović, S., Stanković, M., Erić, P., Erić, K., Patenković, A.,& Tanasković, M.. (2023). Evaluating ND1 and Cytb mitochondrial genes as markers for diversity analysis of protected White-tailed eagle species from Serbia. in Book of Abstracts: 4th Belgrade Bioinformatics Conference: BELBI2023; 2023 Jun 1-23; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5868

Morić I, Đorđević V, Davidović S, Stanković M, Erić P, Erić K, Patenković A, Tanasković M. Evaluating ND1 and Cytb mitochondrial genes as markers for diversity analysis of protected White-tailed eagle species from Serbia. in Book of Abstracts: 4th Belgrade Bioinformatics Conference: BELBI2023; 2023 Jun 1-23; Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5868 .

Morić, Ivana, Đorđević, Valentina, Davidović, Slobodan, Stanković, Milica, Erić, Pavle, Erić, Katarina, Patenković, Aleksandra, Tanasković, Marija, "Evaluating ND1 and Cytb mitochondrial genes as markers for diversity analysis of protected White-tailed eagle species from Serbia" in Book of Abstracts: 4th Belgrade Bioinformatics Conference: BELBI2023; 2023 Jun 1-23; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5868 .

TY  - JOUR
AU  - Erić, Katarina
AU  - Savić Veselinović, Marija
AU  - Patenković, Aleksandra
AU  - Davidović, Slobodan
AU  - Erić, Pavle
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5865
AB  - Drosophila subobscura is considered a good model species for investigation of a population’s ability to adapt and cope with climate changes. Decade long research has shown that inversion frequencies change in response to environmental factors indicating their role in adaptation to novel environments. The mechanisms behind organisms’ responses to temperature are complex, involving changes in physiology, behavior, gene expression and regulation. On the other hand, a population’s ability to respond to suboptimal conditions depends on standing genetic variation and population history. In order to elucidate the role of local adaptation in population response to the changing temperature, we investigated the response to temperature in D. subobscura individuals originating from two different altitudes by combining traditional cytogenetic techniques with assessing the levels of Hsp70 protein expression. Inversion polymorphism was assessed in the flies sampled from natural populations and in flies reared in laboratory conditions at three different temperatures after five and sixteen generations and Hsp70 protein expression profile in 12th generation flies at the basal level and after heat shock induction. Our results indicate that local adaptation and population history influence population response to the changing temperature.
PB  - Basel: MDPI
T2  - Life
T1  - Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura
IS  - 6
VL  - 13
DO  - 10.3390/life13061333
SP  - 1333
ER  - 

@article{
author = "Erić, Katarina and Savić Veselinović, Marija and Patenković, Aleksandra and Davidović, Slobodan and Erić, Pavle and Stamenković-Radak, Marina and Tanasković, Marija",
year = "2023",
abstract = "Drosophila subobscura is considered a good model species for investigation of a population’s ability to adapt and cope with climate changes. Decade long research has shown that inversion frequencies change in response to environmental factors indicating their role in adaptation to novel environments. The mechanisms behind organisms’ responses to temperature are complex, involving changes in physiology, behavior, gene expression and regulation. On the other hand, a population’s ability to respond to suboptimal conditions depends on standing genetic variation and population history. In order to elucidate the role of local adaptation in population response to the changing temperature, we investigated the response to temperature in D. subobscura individuals originating from two different altitudes by combining traditional cytogenetic techniques with assessing the levels of Hsp70 protein expression. Inversion polymorphism was assessed in the flies sampled from natural populations and in flies reared in laboratory conditions at three different temperatures after five and sixteen generations and Hsp70 protein expression profile in 12th generation flies at the basal level and after heat shock induction. Our results indicate that local adaptation and population history influence population response to the changing temperature.",
publisher = "Basel: MDPI",
journal = "Life",
title = "Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura",
number = "6",
volume = "13",
doi = "10.3390/life13061333",
pages = "1333"
}

Erić, K., Savić Veselinović, M., Patenković, A., Davidović, S., Erić, P., Stamenković-Radak, M.,& Tanasković, M.. (2023). Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura. in Life
Basel: MDPI., 13(6), 1333.
https://doi.org/10.3390/life13061333

Erić K, Savić Veselinović M, Patenković A, Davidović S, Erić P, Stamenković-Radak M, Tanasković M. Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura. in Life. 2023;13(6):1333.
doi:10.3390/life13061333 .

Erić, Katarina, Savić Veselinović, Marija, Patenković, Aleksandra, Davidović, Slobodan, Erić, Pavle, Stamenković-Radak, Marina, Tanasković, Marija, "Population History Shapes Responses to Different Temperature Regimes in Drosophila subobscura" in Life, 13, no. 6 (2023):1333,
https://doi.org/10.3390/life13061333 . .

TY  - JOUR
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Mihajlović, Milica
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - https://www.nature.com/articles/s41598-022-21413-y
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5084
AB  - Urbanization can change biodiversity in both directions, positive and negative, and despite the rising global trend of urban beekeeping, little is known about the impact of urbanization on the genetic diversity of honey bees. We investigate how urbanization affects the genetic variability of feral and managed honey bee colonies that are spread throughout the entire city, even in highly urban areas, through genetic analysis of 82 worker bees. We found convincing evidence of high genetic differentiation between these two groups. Additionally, by comparing city samples with 241 samples from 46 apiaries in rural parts of the country, variations in mitochondrial tRNAleu-cox2 intergenic region and microsatellite loci indicated that feral colonies have distinct patterns of genetic diversity. These results, with evidence that feral honey bees find niches within highly modified and human-dominated urban landscapes, lead us to conclude that urbanization is a driver of the genetic diversity of feral honey bees in the city.
PB  - Berlin: Nature Portfolio
T2  - Scientific Reports
T1  - Urban ecosystem drives genetic diversity in feral honey bee.
IS  - 1
VL  - 12
DO  - 10.1038/s41598-022-21413-y
SP  - 17692
ER  - 

@article{
author = "Patenković, Aleksandra and Tanasković, Marija and Erić, Pavle and Erić, Katarina and Mihajlović, Milica and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Urbanization can change biodiversity in both directions, positive and negative, and despite the rising global trend of urban beekeeping, little is known about the impact of urbanization on the genetic diversity of honey bees. We investigate how urbanization affects the genetic variability of feral and managed honey bee colonies that are spread throughout the entire city, even in highly urban areas, through genetic analysis of 82 worker bees. We found convincing evidence of high genetic differentiation between these two groups. Additionally, by comparing city samples with 241 samples from 46 apiaries in rural parts of the country, variations in mitochondrial tRNAleu-cox2 intergenic region and microsatellite loci indicated that feral colonies have distinct patterns of genetic diversity. These results, with evidence that feral honey bees find niches within highly modified and human-dominated urban landscapes, lead us to conclude that urbanization is a driver of the genetic diversity of feral honey bees in the city.",
publisher = "Berlin: Nature Portfolio",
journal = "Scientific Reports",
title = "Urban ecosystem drives genetic diversity in feral honey bee.",
number = "1",
volume = "12",
doi = "10.1038/s41598-022-21413-y",
pages = "17692"
}

Patenković, A., Tanasković, M., Erić, P., Erić, K., Mihajlović, M., Stanisavljević, L.,& Davidović, S.. (2022). Urban ecosystem drives genetic diversity in feral honey bee.. in Scientific Reports
Berlin: Nature Portfolio., 12(1), 17692.
https://doi.org/10.1038/s41598-022-21413-y

Patenković A, Tanasković M, Erić P, Erić K, Mihajlović M, Stanisavljević L, Davidović S. Urban ecosystem drives genetic diversity in feral honey bee.. in Scientific Reports. 2022;12(1):17692.
doi:10.1038/s41598-022-21413-y .

Patenković, Aleksandra, Tanasković, Marija, Erić, Pavle, Erić, Katarina, Mihajlović, Milica, Stanisavljević, Ljubiša, Davidović, Slobodan, "Urban ecosystem drives genetic diversity in feral honey bee." in Scientific Reports, 12, no. 1 (2022):17692,
https://doi.org/10.1038/s41598-022-21413-y . .

TY  - JOUR
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Tanasković, Marija
AU  - Davidović, Slobodan
AU  - Rakić, Mina
AU  - Savić Veselinović, Marija
AU  - Stamenković-Radak, Marina
AU  - Jelić, Mihailo
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4892
AB  - The adaptive significance of sympatric mitochondrial (mtDNA) variation and the role of
selective mechanisms that maintain it are debated to this day. Isofemale lines of Drosophila obscura
collected from four populations were backcrossed within populations to construct experimental
lines, with all combinations of mtDNA Cyt b haplotypes and nuclear genetic backgrounds (nuDNA).
Individuals of both sexes from these lines were then subjected to four fitness assays (desiccation
resistance, developmental time, egg-to-adult viability and sex ratio) on two experimental temperatures to examine the role of temperature fluctuations and sex-specific selection, as well as the part
that interactions between the two genomes play in shaping mtDNA variation. The results varied
across populations and fitness components. In the majority of comparisons, they show that sympatric
mitochondrial variants affect fitness. However, their effect should be examined in light of interactions
with nuDNA, as mito-nuclear genotype was even more influential on fitness across all components.
We found both sex-specific and temperature-specific differences in mitochondrial and mito-nuclear
genotype ranks in all fitness components. The effect of temperature-specific selection was found to
be more prominent, especially in desiccation resistance. From the results of different components
tested, we can also infer that temperature-specific mito-nuclear interactions rather than sex-specific
selection on mito-nuclear genotypes have a more substantial role in preserving mtDNA variation in
this model species.
PB  - Basel: MDPI
T2  - Insects
T1  - Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura
IS  - 2
VL  - 13
DO  - 10.3390/insects13020139
SP  - 139
ER  - 

@article{
author = "Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Tanasković, Marija and Davidović, Slobodan and Rakić, Mina and Savić Veselinović, Marija and Stamenković-Radak, Marina and Jelić, Mihailo",
year = "2022",
abstract = "The adaptive significance of sympatric mitochondrial (mtDNA) variation and the role of
selective mechanisms that maintain it are debated to this day. Isofemale lines of Drosophila obscura
collected from four populations were backcrossed within populations to construct experimental
lines, with all combinations of mtDNA Cyt b haplotypes and nuclear genetic backgrounds (nuDNA).
Individuals of both sexes from these lines were then subjected to four fitness assays (desiccation
resistance, developmental time, egg-to-adult viability and sex ratio) on two experimental temperatures to examine the role of temperature fluctuations and sex-specific selection, as well as the part
that interactions between the two genomes play in shaping mtDNA variation. The results varied
across populations and fitness components. In the majority of comparisons, they show that sympatric
mitochondrial variants affect fitness. However, their effect should be examined in light of interactions
with nuDNA, as mito-nuclear genotype was even more influential on fitness across all components.
We found both sex-specific and temperature-specific differences in mitochondrial and mito-nuclear
genotype ranks in all fitness components. The effect of temperature-specific selection was found to
be more prominent, especially in desiccation resistance. From the results of different components
tested, we can also infer that temperature-specific mito-nuclear interactions rather than sex-specific
selection on mito-nuclear genotypes have a more substantial role in preserving mtDNA variation in
this model species.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura",
number = "2",
volume = "13",
doi = "10.3390/insects13020139",
pages = "139"
}

Erić, P., Patenković, A., Erić, K., Tanasković, M., Davidović, S., Rakić, M., Savić Veselinović, M., Stamenković-Radak, M.,& Jelić, M.. (2022). Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura. in Insects
Basel: MDPI., 13(2), 139.
https://doi.org/10.3390/insects13020139

Erić P, Patenković A, Erić K, Tanasković M, Davidović S, Rakić M, Savić Veselinović M, Stamenković-Radak M, Jelić M. Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura. in Insects. 2022;13(2):139.
doi:10.3390/insects13020139 .

Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Tanasković, Marija, Davidović, Slobodan, Rakić, Mina, Savić Veselinović, Marija, Stamenković-Radak, Marina, Jelić, Mihailo, "Temperature-Specific and Sex-Specific Fitness Effects of Sympatric Mitochondrial and Mito-Nuclear Variation in Drosophila obscura" in Insects, 13, no. 2 (2022):139,
https://doi.org/10.3390/insects13020139 . .

TY  - JOUR
AU  - Erić, Pavle
AU  - Stamenković-Radak, Marina
AU  - Dragićević, Milan
AU  - Kankare, Maaria
AU  - Wallace, Megan A.
AU  - Savić Veselinović, Marija
AU  - Jelić, Mihailo
PY  - 2022
UR  - http://www.eje.cz/doi/10.14411/eje.2022.011.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4872
AB  - Drosophila obscura is a common fruit fly that inhabits the temperate forests of Europe. While it is abundant in the north compared to other Drosophila, its density decreases southwards, where it is gradually replaced by other Drosophila species. This study describes variation in the mitochondrial Cyt b gene of D. obscura from several European populations. We observed a large number of haplotypes, together with the structuring of genetic variation. Genetic variation is higher in the west where O1 and related divergent haplotypes dominate. In the east, the O2 haplotype is most frequent, together with haplotypes that recently arose from it. In the central part of the species range, both O1 and O2 are equally present, along with many others. These data reveal signs of population expansions that probably happened earlier in the west, and more recently in the east. Though our conclusions are based on only one genetic marker, limiting the power of the analysis, the results imply either postglacial expansion from two unique sources or, more likely, eastwards stepping-stone expansion. This study adds important information on genetic variation and phylogeography to the obscure biology of D. obscura, a species that has the potential to become an interesting model in evolutionary biology and conservation genetics.
PB  - České Budějovice: Czech Academy of Sciences
T2  - European Journal of Entomology
T1  - Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe
VL  - 119
DO  - 10.14411/eje.2022.011
SP  - 99
EP  - 110
ER  - 

@article{
author = "Erić, Pavle and Stamenković-Radak, Marina and Dragićević, Milan and Kankare, Maaria and Wallace, Megan A. and Savić Veselinović, Marija and Jelić, Mihailo",
year = "2022",
abstract = "Drosophila obscura is a common fruit fly that inhabits the temperate forests of Europe. While it is abundant in the north compared to other Drosophila, its density decreases southwards, where it is gradually replaced by other Drosophila species. This study describes variation in the mitochondrial Cyt b gene of D. obscura from several European populations. We observed a large number of haplotypes, together with the structuring of genetic variation. Genetic variation is higher in the west where O1 and related divergent haplotypes dominate. In the east, the O2 haplotype is most frequent, together with haplotypes that recently arose from it. In the central part of the species range, both O1 and O2 are equally present, along with many others. These data reveal signs of population expansions that probably happened earlier in the west, and more recently in the east. Though our conclusions are based on only one genetic marker, limiting the power of the analysis, the results imply either postglacial expansion from two unique sources or, more likely, eastwards stepping-stone expansion. This study adds important information on genetic variation and phylogeography to the obscure biology of D. obscura, a species that has the potential to become an interesting model in evolutionary biology and conservation genetics.",
publisher = "České Budějovice: Czech Academy of Sciences",
journal = "European Journal of Entomology",
title = "Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe",
volume = "119",
doi = "10.14411/eje.2022.011",
pages = "99-110"
}

Erić, P., Stamenković-Radak, M., Dragićević, M., Kankare, M., Wallace, M. A., Savić Veselinović, M.,& Jelić, M.. (2022). Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe. in European Journal of Entomology
České Budějovice: Czech Academy of Sciences., 119, 99-110.
https://doi.org/10.14411/eje.2022.011

Erić P, Stamenković-Radak M, Dragićević M, Kankare M, Wallace MA, Savić Veselinović M, Jelić M. Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe. in European Journal of Entomology. 2022;119:99-110.
doi:10.14411/eje.2022.011 .

Erić, Pavle, Stamenković-Radak, Marina, Dragićević, Milan, Kankare, Maaria, Wallace, Megan A., Savić Veselinović, Marija, Jelić, Mihailo, "Mitochondrial DNA variation of Drosophila obscura (Diptera: Drosophilidae) across Europe" in European Journal of Entomology, 119 (2022):99-110,
https://doi.org/10.14411/eje.2022.011 . .

TY  - JOUR
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Kusza, Szilvia
AU  - Oleksa, Andrzej
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4806
AB  - Socioeconomic interests and beekeeper preferences have often taken precedence over the conservation of locally native honey bee subspecies, leading to the predominance of admixture populations in human-dominated areas. To assess the genetic diversity of contemporary managed Serbian honey bee colonies, we used 14 microsatellite loci and analyzed 237 worker bees from 46 apiaries in eight localities of northern and southern Serbia. Furthermore, we compared data for nine microsatellite loci with 338 individuals from Italy, Hungary, Poland, and Spain. The standard parameters of genetic diversity in Serbian honey bee populations were in line with other analyses, although somewhat smaller. STRUCTURE analysis showed the existence of two equally distributed genetic clusters and Analysis of molecular variances could not confirm the presence of a geographically discrete population but showed local differences. Discriminant analysis of principal components showed overlapping of worker bees from different parts of Serbia. Clear genetic differentiation can be observed when comparing all populations between geographical regions and their corresponding subspecies. The absence of the A. m. macedonica subspecies from its historical distribution range in southern Serbia as well as the lack of distinctive geographical groups suggest that selective breeding, queen import, and migratory beekeeping practices strongly influence the genetic structure and diversity of honey bees, leading to the genetic uniformization and creation of the admixture population.
PB  - Basel: MDPI
T2  - Insects
T1  - Further Evidence of Population Admixture in the Serbian Honey Bee Population
IS  - 2
VL  - 13
DO  - 10.3390/insects13020180
SP  - 180
ER  - 

@article{
author = "Tanasković, Marija and Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Mihajlović, Milica and Tanasić, Vanja and Kusza, Szilvia and Oleksa, Andrzej and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Socioeconomic interests and beekeeper preferences have often taken precedence over the conservation of locally native honey bee subspecies, leading to the predominance of admixture populations in human-dominated areas. To assess the genetic diversity of contemporary managed Serbian honey bee colonies, we used 14 microsatellite loci and analyzed 237 worker bees from 46 apiaries in eight localities of northern and southern Serbia. Furthermore, we compared data for nine microsatellite loci with 338 individuals from Italy, Hungary, Poland, and Spain. The standard parameters of genetic diversity in Serbian honey bee populations were in line with other analyses, although somewhat smaller. STRUCTURE analysis showed the existence of two equally distributed genetic clusters and Analysis of molecular variances could not confirm the presence of a geographically discrete population but showed local differences. Discriminant analysis of principal components showed overlapping of worker bees from different parts of Serbia. Clear genetic differentiation can be observed when comparing all populations between geographical regions and their corresponding subspecies. The absence of the A. m. macedonica subspecies from its historical distribution range in southern Serbia as well as the lack of distinctive geographical groups suggest that selective breeding, queen import, and migratory beekeeping practices strongly influence the genetic structure and diversity of honey bees, leading to the genetic uniformization and creation of the admixture population.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "Further Evidence of Population Admixture in the Serbian Honey Bee Population",
number = "2",
volume = "13",
doi = "10.3390/insects13020180",
pages = "180"
}

Tanasković, M., Erić, P., Patenković, A., Erić, K., Mihajlović, M., Tanasić, V., Kusza, S., Oleksa, A., Stanisavljević, L.,& Davidović, S.. (2022). Further Evidence of Population Admixture in the Serbian Honey Bee Population. in Insects
Basel: MDPI., 13(2), 180.
https://doi.org/10.3390/insects13020180

Tanasković M, Erić P, Patenković A, Erić K, Mihajlović M, Tanasić V, Kusza S, Oleksa A, Stanisavljević L, Davidović S. Further Evidence of Population Admixture in the Serbian Honey Bee Population. in Insects. 2022;13(2):180.
doi:10.3390/insects13020180 .

Tanasković, Marija, Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Mihajlović, Milica, Tanasić, Vanja, Kusza, Szilvia, Oleksa, Andrzej, Stanisavljević, Ljubiša, Davidović, Slobodan, "Further Evidence of Population Admixture in the Serbian Honey Bee Population" in Insects, 13, no. 2 (2022):180,
https://doi.org/10.3390/insects13020180 . .

TY  - JOUR
AU  - Erić, Katarina
AU  - Patenković, Aleksandra
AU  - Erić, Pavle
AU  - Davidović, Slobodan
AU  - Savić Veselinović, Marija
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
PY  - 2022
UR  - https://www.mdpi.com/2075-4450/13/2/138
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4800
AB  - Global warming and climate change are affecting many insect species in numerous ways. These species can develop diverse mechanisms as a response to variable environmental conditions. The rise in mean and extreme temperatures due to global warming and the importance of the population’s ability to adapt to temperature stress will further increase. In this study, we investigated thermal stress response, which is considered to be one of the crucial elements of population fitness and survival in fast-changing environments. The dynamics and variation of thermal stress resistance traits in D. subobscura flies originating from two natural populations sampled from different altitudes were analysed. Three different temperature regimes (25 °C, 19 °C, and 16 °C) were used for the F1 progeny from both localities to establish six experimental groups and investigate stress resistance traits: desiccation resistance, heat knock-down resistance, starvation resistance, and chill-coma recovery time. We detected that laboratory thermal conditions and population origin may have an effect on the analysed traits, and that sex also significantly influences stress resistance. Individuals from the lower altitude reared at higher temperatures show inferior resistance to thermal shock.
PB  - Basel: MDPI
T2  - Insects
T1  - Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes
IS  - 2
VL  - 13
DO  - 10.3390/insects13020138
SP  - 138
ER  - 

@article{
author = "Erić, Katarina and Patenković, Aleksandra and Erić, Pavle and Davidović, Slobodan and Savić Veselinović, Marija and Stamenković-Radak, Marina and Tanasković, Marija",
year = "2022",
abstract = "Global warming and climate change are affecting many insect species in numerous ways. These species can develop diverse mechanisms as a response to variable environmental conditions. The rise in mean and extreme temperatures due to global warming and the importance of the population’s ability to adapt to temperature stress will further increase. In this study, we investigated thermal stress response, which is considered to be one of the crucial elements of population fitness and survival in fast-changing environments. The dynamics and variation of thermal stress resistance traits in D. subobscura flies originating from two natural populations sampled from different altitudes were analysed. Three different temperature regimes (25 °C, 19 °C, and 16 °C) were used for the F1 progeny from both localities to establish six experimental groups and investigate stress resistance traits: desiccation resistance, heat knock-down resistance, starvation resistance, and chill-coma recovery time. We detected that laboratory thermal conditions and population origin may have an effect on the analysed traits, and that sex also significantly influences stress resistance. Individuals from the lower altitude reared at higher temperatures show inferior resistance to thermal shock.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes",
number = "2",
volume = "13",
doi = "10.3390/insects13020138",
pages = "138"
}

Erić, K., Patenković, A., Erić, P., Davidović, S., Savić Veselinović, M., Stamenković-Radak, M.,& Tanasković, M.. (2022). Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes. in Insects
Basel: MDPI., 13(2), 138.
https://doi.org/10.3390/insects13020138

Erić K, Patenković A, Erić P, Davidović S, Savić Veselinović M, Stamenković-Radak M, Tanasković M. Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes. in Insects. 2022;13(2):138.
doi:10.3390/insects13020138 .

Erić, Katarina, Patenković, Aleksandra, Erić, Pavle, Davidović, Slobodan, Savić Veselinović, Marija, Stamenković-Radak, Marina, Tanasković, Marija, "Stress Resistance Traits under Different Thermal Conditions in Drosophila subobscura from Two Altitudes" in Insects, 13, no. 2 (2022):138,
https://doi.org/10.3390/insects13020138 . .

TY  - CONF
AU  - Erić, Katarina
AU  - Savić Veselinović, Marija
AU  - Patenković, Aleksandra
AU  - Erić, Pavle
AU  - Jelić, Miahilo
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
PY  - 2022
UR  - https://www.eseb2022.cz/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5095
AB  - Drosophila subobscura is a wide spread Palearctic species, which possesses very rich
inversion polymorphism in all of the five acrocentric chromosomes. As populations rapid
response to fast changes of environmental conditions can be seen through changes in
inversions frequency, this polymorphism is good candidate to measure the effect of
temperature on the genetic structure of population. Frequencies of particular
chromosomal arrangements are correlated with temperature, with standard
arrangements increasing in frequency at lower temperatures, and more complex
arrangements like J1, U1+2, U1+2+6, E1+2+9, and O3+4 increasing in frequency at higher
temperatures. In order to explore the effect of optimal and suboptimal temperatures on
the arrangements frequencies, samples of D. subobscura population from a high altitude
were reared in laboratory at suboptimal (16°C) and optimal (19°C). Chromosome
arrangements frequencies were analyzed and compared through generations (F0, F5, and
F16). The increasing frequency of complex chromosome arrangements and the
decreasing frequency of standard chromosomal arrangements were observed. There are
significant differences in some of the chromosome arrangements between suboptimal
and optimal conditions and also between generations, indicating laboratory adaptation
through generations.
PB  - European Society for Evolutionary Biology
PB  - e
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
T1  - Temperature shapes chromosome inversion polymorphism of Drosophila subobscura through generations
SP  - 759
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5095
ER  - 

@conference{
author = "Erić, Katarina and Savić Veselinović, Marija and Patenković, Aleksandra and Erić, Pavle and Jelić, Miahilo and Stamenković-Radak, Marina and Tanasković, Marija",
year = "2022",
abstract = "Drosophila subobscura is a wide spread Palearctic species, which possesses very rich
inversion polymorphism in all of the five acrocentric chromosomes. As populations rapid
response to fast changes of environmental conditions can be seen through changes in
inversions frequency, this polymorphism is good candidate to measure the effect of
temperature on the genetic structure of population. Frequencies of particular
chromosomal arrangements are correlated with temperature, with standard
arrangements increasing in frequency at lower temperatures, and more complex
arrangements like J1, U1+2, U1+2+6, E1+2+9, and O3+4 increasing in frequency at higher
temperatures. In order to explore the effect of optimal and suboptimal temperatures on
the arrangements frequencies, samples of D. subobscura population from a high altitude
were reared in laboratory at suboptimal (16°C) and optimal (19°C). Chromosome
arrangements frequencies were analyzed and compared through generations (F0, F5, and
F16). The increasing frequency of complex chromosome arrangements and the
decreasing frequency of standard chromosomal arrangements were observed. There are
significant differences in some of the chromosome arrangements between suboptimal
and optimal conditions and also between generations, indicating laboratory adaptation
through generations.",
publisher = "European Society for Evolutionary Biology, e",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic",
title = "Temperature shapes chromosome inversion polymorphism of Drosophila subobscura through generations",
pages = "759",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5095"
}

Erić, K., Savić Veselinović, M., Patenković, A., Erić, P., Jelić, M., Stamenković-Radak, M.,& Tanasković, M.. (2022). Temperature shapes chromosome inversion polymorphism of Drosophila subobscura through generations. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
European Society for Evolutionary Biology., 759.
https://hdl.handle.net/21.15107/rcub_ibiss_5095

Erić K, Savić Veselinović M, Patenković A, Erić P, Jelić M, Stamenković-Radak M, Tanasković M. Temperature shapes chromosome inversion polymorphism of Drosophila subobscura through generations. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic. 2022;:759.
https://hdl.handle.net/21.15107/rcub_ibiss_5095 .

Erić, Katarina, Savić Veselinović, Marija, Patenković, Aleksandra, Erić, Pavle, Jelić, Miahilo, Stamenković-Radak, Marina, Tanasković, Marija, "Temperature shapes chromosome inversion polymorphism of Drosophila subobscura through generations" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic (2022):759,
https://hdl.handle.net/21.15107/rcub_ibiss_5095 .

TY  - DATA
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Mihailović, Milica
AU  - Tanasić, Vanja
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4954
AB  - Figure S5. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 14 microsatellite loci (A7, A8, A14, A24, A28, A35, A43, A79, A88, A107, A113, Ap43, Ap249 and B124) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica), South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia including the Belgrade and for the feral honey bees that were found on different locations in Belgrade. In figure S5 the first three principal components (PC) are presented.
T1  - Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee"
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4954
ER  - 

@misc{
author = "Patenković, Aleksandra and Tanasković, Marija and Erić, Pavle and Erić, Katarina and Mihailović, Milica and Tanasić, Vanja and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Figure S5. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 14 microsatellite loci (A7, A8, A14, A24, A28, A35, A43, A79, A88, A107, A113, Ap43, Ap249 and B124) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica), South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia including the Belgrade and for the feral honey bees that were found on different locations in Belgrade. In figure S5 the first three principal components (PC) are presented.",
title = "Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee"",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4954"
}

Patenković, A., Tanasković, M., Erić, P., Erić, K., Mihailović, M., Tanasić, V., Stanisavljević, L.,& Davidović, S.. (2022). Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee". .
https://hdl.handle.net/21.15107/rcub_ibiss_4954

Patenković A, Tanasković M, Erić P, Erić K, Mihailović M, Tanasić V, Stanisavljević L, Davidović S. Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee". 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_4954 .

Patenković, Aleksandra, Tanasković, Marija, Erić, Pavle, Erić, Katarina, Mihailović, Milica, Tanasić, Vanja, Stanisavljević, Ljubiša, Davidović, Slobodan, "Supplementary Material "Urban ecosystem drives genetic diversity in feral honey bee"" (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_4954 .

TY  - CONF
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5039
AB  - Medonosna pčela (Apis mellifera) u Srbiji je poslednjih decenija pod uticajem čoveka usled ekspanzije pčelarstva. Pored naglog povećanja broja pčelara, košnica i selekcionih centara, savremene pčelarske tehnike uključuju i trgovinu maticama, veštačku selekciju i migratorno pčelarstvo, što utiče i na genetičku raznovrsnost gajenih i feralnih populacija ove ekološki i ekonomski važne vrste. Uporedne analize nuklearnog i mitohondrijskog (mtDNK) genoma radilica iz feralnih društava (56) i pčelinjaka (542) širom Srbije, pokazale su da feralna društva imaju veće vrednosti parametara genetičke raznovrsnosti za oba genetička markera u poređenju sa gajenim pčelama. Procene srodnosti su pokazale da su beogradska feralna društva međusobno srodnija nego sa drugim gajenim društvima sa teritorije Beograda, što ukazuje na dobro uspostavljenu populaciju feralnih pčela u glavnom gradu. Rezultati analize mtDNK ukazuju na značajnu promenu u distribuciji i raznovrsnosti mtDNK linija gajenih pčela u poređenju sa podacima za Srbiju iz ranijih decenija, a rezultati analize polimorfnosti 14 mikrosatelitska lokusa - na gubitak genetičke raznovrsnosti i veliku uniformnost gajenih pčela. Ovo ukazuje na urgentnu potrebu za promenom pčelarske prakse i za očuvanjem kako lokalno adaptiranih populacija, tj. različitih ekotipova karakterističnih za određene regione Srbije, tako i feralnih populacija, čime bi se obezbedila zaštita genetičkog diverziteta i opstanak medonosne pčele u Srbiji.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Medonosne pčele Srbije – populaciono-genetički pogled
SP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5039
ER  - 

@conference{
author = "Patenković, Aleksandra and Tanasković, Marija and Erić, Pavle and Erić, Katarina and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Medonosna pčela (Apis mellifera) u Srbiji je poslednjih decenija pod uticajem čoveka usled ekspanzije pčelarstva. Pored naglog povećanja broja pčelara, košnica i selekcionih centara, savremene pčelarske tehnike uključuju i trgovinu maticama, veštačku selekciju i migratorno pčelarstvo, što utiče i na genetičku raznovrsnost gajenih i feralnih populacija ove ekološki i ekonomski važne vrste. Uporedne analize nuklearnog i mitohondrijskog (mtDNK) genoma radilica iz feralnih društava (56) i pčelinjaka (542) širom Srbije, pokazale su da feralna društva imaju veće vrednosti parametara genetičke raznovrsnosti za oba genetička markera u poređenju sa gajenim pčelama. Procene srodnosti su pokazale da su beogradska feralna društva međusobno srodnija nego sa drugim gajenim društvima sa teritorije Beograda, što ukazuje na dobro uspostavljenu populaciju feralnih pčela u glavnom gradu. Rezultati analize mtDNK ukazuju na značajnu promenu u distribuciji i raznovrsnosti mtDNK linija gajenih pčela u poređenju sa podacima za Srbiju iz ranijih decenija, a rezultati analize polimorfnosti 14 mikrosatelitska lokusa - na gubitak genetičke raznovrsnosti i veliku uniformnost gajenih pčela. Ovo ukazuje na urgentnu potrebu za promenom pčelarske prakse i za očuvanjem kako lokalno adaptiranih populacija, tj. različitih ekotipova karakterističnih za određene regione Srbije, tako i feralnih populacija, čime bi se obezbedila zaštita genetičkog diverziteta i opstanak medonosne pčele u Srbiji.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Medonosne pčele Srbije – populaciono-genetički pogled",
pages = "87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5039"
}

Patenković, A., Tanasković, M., Erić, P., Erić, K., Stanisavljević, L.,& Davidović, S.. (2022). Medonosne pčele Srbije – populaciono-genetički pogled. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 87.
https://hdl.handle.net/21.15107/rcub_ibiss_5039

Patenković A, Tanasković M, Erić P, Erić K, Stanisavljević L, Davidović S. Medonosne pčele Srbije – populaciono-genetički pogled. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:87.
https://hdl.handle.net/21.15107/rcub_ibiss_5039 .

Patenković, Aleksandra, Tanasković, Marija, Erić, Pavle, Erić, Katarina, Stanisavljević, Ljubiša, Davidović, Slobodan, "Medonosne pčele Srbije – populaciono-genetički pogled" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):87,
https://hdl.handle.net/21.15107/rcub_ibiss_5039 .

TY  - CONF
AU  - Erić, Pavle
AU  - Tanasković, Marija
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5037
AB  - Zapadna medonosna pčela je kosmopolit i jedan od najznačajnih oprašivača na planeti zemlji. Smatra se da je vrsta nastala na afričkom kontinentu odakle se prirodno proširila u Evropu, Bliski istok i zapadne delove Azije. Čovek je shvativši ekonomski značaj ove vrste medonosnu pčelu preneo u Severnu i Južnu Ameriku, istočnu Aziju, Australiju i Novi Zeland. Medonosna pčela se šireći svoj areal prilagoñavala novim životnim sredinama, sinhronizujući svoje aktivnosti sa ciklusima cvetanja lokalnih biljaka, kao i sa klimatskim faktorima u novim sredinama, pa tako imamo 33 opisane podvrste Apis mellifera. Ove podvrste podeljene su u 5 evolutivnih linija označenih sa A, M, C, O i Y, korišćenjem morfometrijskih kao i genetičkih markera. Linija A je karakteristična za Afrički kontinent, dok su M i C karakteristične za Severozapadnu i Jugoistočnu Evropu. Linije O i Y karakteristične su za Bliski istok.
U okviru programa za izvrsne projekte mladih istraživača (PROMIS), finansiranog od strane fonda za nauku Republike Srbije realizovan je projekat istraživanja genetičke raznovrsnosti gajenih i divljih društava medonosne pčele Apis mellifera (SERBHIWE). Tokom 2020 i 2021 godine, organizovan je terenski rad na kome su za genetička istraživanja uzorkovani pčelinjaci poreklom sa juga, severa, istoka i zapada Srbije. Uporedo su uzorkovani i pčelinjaci sa teritorije Beograda. Za vreme trajanja projekta uzorkovano je ukupno 102 pčelinjaka i 930 pčelinjih društava (košnica), od kojih je 542 odabrano za analizu genetičke raznovrsnosti mitohondrijske DNK.
Kako bi što bolje prezentovali genetičku raznovrsnost karakterističnu za lokalno geografsko podneblje za uzorkovanje su odabirani stacionarni pčelinjaci u koje idealno nisu donošene matice sa strane tokom dužeg vremenskog perioda.
Cilj projekta SERBHIWE je da se definiše genetička struktura populacije medonosnih pčela u Srbiji, kao i da se na genetičkom nivou potencijalno prepoznaju lokalno adaptirane podvrste, ekotipovi i populacije. Sekvenciranjem COI-COII intergenskog regiona kod pčela iz Srbije zabeleženo je 20 mitohondrijskih haplotipova od kojih je čak 10 novih za nauku. Najčešće detektovani haplotip pripada liniji C2d, i pronañen je na svih 18 lokaliteta. Sledeći po učestalosti je haplotip C2e koji je otkriven kod jedinki iz 16 lokaliteta, a zatim C1a koji je zabeležen na 12 lokaliteta. Iako je zabeležen značajna genetička raznovrsnost, nisu uočene visoke genetičke razlike koje bi jsano odvojile populacije iz različitih delova Srbije. Dodatno, korišćenjem mitohondrijske DNK nismo uspeli da utvrdimo postojanje lokalnih ekotipova karakterističnih samo za odreñeno podneblje.
Pored analize tRNAleu-cox2 intergenskog regiona, koristili smo i PCR-RFLP metodu kojom se jasno može izdvojiti A. m. macedonica koja sa A. m. carnica, čini dve podvrste medonosne pčele koje su kao autohtone bile zabeležene na teritoriji Republike Srbije.
Iako prethodni radovi, pokazuju prisustvo A. m. carnica u Vojvodini, i gotovo preovlañujuće prisustvo A. m. macedonica u južnoj Srbiji, sa zonom hibridizacije izmeñu, mi nismo uspeli ni u jednom od (542) uzorka da dobijemo restrikcioni obrazac karakterističan za ovu podvrstu. Razlog tome možda leži u tome što je prema zakonu o stočarstvu Republike Srbije dozvoljeno gajenje samo A. m. carnica, kao i celokupnoj promeni pčelarske prakse, gde migratorno pčelarstvo postaje sve zastupljenije, dok se proizvodnja matica razrojavanjem od sopstvenih društava sve više napušta u korist kupovine komercijalno uzgojenih matica.
PB  - Belgrade : Entomological Society of Serbia
C3  - Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi
T1  - Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije
SP  - 48
EP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5037
ER  - 

@conference{
author = "Erić, Pavle and Tanasković, Marija and Patenković, Aleksandra and Erić, Katarina and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Zapadna medonosna pčela je kosmopolit i jedan od najznačajnih oprašivača na planeti zemlji. Smatra se da je vrsta nastala na afričkom kontinentu odakle se prirodno proširila u Evropu, Bliski istok i zapadne delove Azije. Čovek je shvativši ekonomski značaj ove vrste medonosnu pčelu preneo u Severnu i Južnu Ameriku, istočnu Aziju, Australiju i Novi Zeland. Medonosna pčela se šireći svoj areal prilagoñavala novim životnim sredinama, sinhronizujući svoje aktivnosti sa ciklusima cvetanja lokalnih biljaka, kao i sa klimatskim faktorima u novim sredinama, pa tako imamo 33 opisane podvrste Apis mellifera. Ove podvrste podeljene su u 5 evolutivnih linija označenih sa A, M, C, O i Y, korišćenjem morfometrijskih kao i genetičkih markera. Linija A je karakteristična za Afrički kontinent, dok su M i C karakteristične za Severozapadnu i Jugoistočnu Evropu. Linije O i Y karakteristične su za Bliski istok.
U okviru programa za izvrsne projekte mladih istraživača (PROMIS), finansiranog od strane fonda za nauku Republike Srbije realizovan je projekat istraživanja genetičke raznovrsnosti gajenih i divljih društava medonosne pčele Apis mellifera (SERBHIWE). Tokom 2020 i 2021 godine, organizovan je terenski rad na kome su za genetička istraživanja uzorkovani pčelinjaci poreklom sa juga, severa, istoka i zapada Srbije. Uporedo su uzorkovani i pčelinjaci sa teritorije Beograda. Za vreme trajanja projekta uzorkovano je ukupno 102 pčelinjaka i 930 pčelinjih društava (košnica), od kojih je 542 odabrano za analizu genetičke raznovrsnosti mitohondrijske DNK.
Kako bi što bolje prezentovali genetičku raznovrsnost karakterističnu za lokalno geografsko podneblje za uzorkovanje su odabirani stacionarni pčelinjaci u koje idealno nisu donošene matice sa strane tokom dužeg vremenskog perioda.
Cilj projekta SERBHIWE je da se definiše genetička struktura populacije medonosnih pčela u Srbiji, kao i da se na genetičkom nivou potencijalno prepoznaju lokalno adaptirane podvrste, ekotipovi i populacije. Sekvenciranjem COI-COII intergenskog regiona kod pčela iz Srbije zabeleženo je 20 mitohondrijskih haplotipova od kojih je čak 10 novih za nauku. Najčešće detektovani haplotip pripada liniji C2d, i pronañen je na svih 18 lokaliteta. Sledeći po učestalosti je haplotip C2e koji je otkriven kod jedinki iz 16 lokaliteta, a zatim C1a koji je zabeležen na 12 lokaliteta. Iako je zabeležen značajna genetička raznovrsnost, nisu uočene visoke genetičke razlike koje bi jsano odvojile populacije iz različitih delova Srbije. Dodatno, korišćenjem mitohondrijske DNK nismo uspeli da utvrdimo postojanje lokalnih ekotipova karakterističnih samo za odreñeno podneblje.
Pored analize tRNAleu-cox2 intergenskog regiona, koristili smo i PCR-RFLP metodu kojom se jasno može izdvojiti A. m. macedonica koja sa A. m. carnica, čini dve podvrste medonosne pčele koje su kao autohtone bile zabeležene na teritoriji Republike Srbije.
Iako prethodni radovi, pokazuju prisustvo A. m. carnica u Vojvodini, i gotovo preovlañujuće prisustvo A. m. macedonica u južnoj Srbiji, sa zonom hibridizacije izmeñu, mi nismo uspeli ni u jednom od (542) uzorka da dobijemo restrikcioni obrazac karakterističan za ovu podvrstu. Razlog tome možda leži u tome što je prema zakonu o stočarstvu Republike Srbije dozvoljeno gajenje samo A. m. carnica, kao i celokupnoj promeni pčelarske prakse, gde migratorno pčelarstvo postaje sve zastupljenije, dok se proizvodnja matica razrojavanjem od sopstvenih društava sve više napušta u korist kupovine komercijalno uzgojenih matica.",
publisher = "Belgrade : Entomological Society of Serbia",
journal = "Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi",
title = "Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije",
pages = "48-49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5037"
}

Erić, P., Tanasković, M., Patenković, A., Erić, K., Stanisavljević, L.,& Davidović, S.. (2022). Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi
Belgrade : Entomological Society of Serbia., 48-49.
https://hdl.handle.net/21.15107/rcub_ibiss_5037

Erić P, Tanasković M, Patenković A, Erić K, Stanisavljević L, Davidović S. Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi. 2022;:48-49.
https://hdl.handle.net/21.15107/rcub_ibiss_5037 .

Erić, Pavle, Tanasković, Marija, Patenković, Aleksandra, Erić, Katarina, Stanisavljević, Ljubiša, Davidović, Slobodan, "Genetička varijabilnost mitohondrijske dnk gajenih društava Apis mellifera iz Srbije" in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbi (2022):48-49,
https://hdl.handle.net/21.15107/rcub_ibiss_5037 .

TY  - CONF
AU  - Erić, Pavle
AU  - Tanasković, Marija
AU  - Erić, Katarina
AU  - Patenković, Aleksandra
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - https://www.eseb2022.cz/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5035
AB  - The western honey bee (Apis mellifera) is one of natures’ most important pollinators and domesticated species. Through human management, it has spread all around the world becoming a cosmopolitan species, and its genetic diversity has been shaped over millennia by anthropogenic influence. Six main mtDNA lineages (M, C, O, Y, A and S) with a large number of existing sublineages are recognized, usually associated with appropriate subspecies. Current nomenclature is inconsistent and the naming of newly identified haplotypes is not standardized. Here we reassessed the nomenclature of C lineage using sequences of the tRNAleu-cox2 intergenic region and complete mitogenomes. Most of the available honey bees’ mtDNA haplotypes are based on the sequence variability of this region while available complete mitogenomes are still few. The first complete mitogenome of Apis mellifera (NC_001566) was used as the reference genome for haplotype determination. We propose that the naming of the mtDNA haplotypes should be performed based on the differences to the reference mitogenome and according to the phylogenetic relationship between detected haplotypes. Our analyses showed that the current nomenclature of C sublineages is not consistent with the phylogenetic relationships between existing haplotypes and that the reassessment followed by the proper naming of haplotypes is needed. We propose to keep the names of the main lineages but correct the names of sublineages using the same approach as in the identification of human mtDNA lineages. This newly standardized nomenclature will bring more order to the studies of honey bees’ genetic diversity.
PB  - European Society for Evolutionary Biology
C3  - Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
T1  - Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny
SP  - 753
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5035
ER  - 

@conference{
author = "Erić, Pavle and Tanasković, Marija and Erić, Katarina and Patenković, Aleksandra and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "The western honey bee (Apis mellifera) is one of natures’ most important pollinators and domesticated species. Through human management, it has spread all around the world becoming a cosmopolitan species, and its genetic diversity has been shaped over millennia by anthropogenic influence. Six main mtDNA lineages (M, C, O, Y, A and S) with a large number of existing sublineages are recognized, usually associated with appropriate subspecies. Current nomenclature is inconsistent and the naming of newly identified haplotypes is not standardized. Here we reassessed the nomenclature of C lineage using sequences of the tRNAleu-cox2 intergenic region and complete mitogenomes. Most of the available honey bees’ mtDNA haplotypes are based on the sequence variability of this region while available complete mitogenomes are still few. The first complete mitogenome of Apis mellifera (NC_001566) was used as the reference genome for haplotype determination. We propose that the naming of the mtDNA haplotypes should be performed based on the differences to the reference mitogenome and according to the phylogenetic relationship between detected haplotypes. Our analyses showed that the current nomenclature of C sublineages is not consistent with the phylogenetic relationships between existing haplotypes and that the reassessment followed by the proper naming of haplotypes is needed. We propose to keep the names of the main lineages but correct the names of sublineages using the same approach as in the identification of human mtDNA lineages. This newly standardized nomenclature will bring more order to the studies of honey bees’ genetic diversity.",
publisher = "European Society for Evolutionary Biology",
journal = "Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic",
title = "Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny",
pages = "753",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5035"
}

Erić, P., Tanasković, M., Erić, K., Patenković, A., Stanisavljević, L.,& Davidović, S.. (2022). Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny. in Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
European Society for Evolutionary Biology., 753.
https://hdl.handle.net/21.15107/rcub_ibiss_5035

Erić P, Tanasković M, Erić K, Patenković A, Stanisavljević L, Davidović S. Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny. in Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic. 2022;:753.
https://hdl.handle.net/21.15107/rcub_ibiss_5035 .

Erić, Pavle, Tanasković, Marija, Erić, Katarina, Patenković, Aleksandra, Stanisavljević, Ljubiša, Davidović, Slobodan, "Toward proper nomenclature: reassessing honeybee (Apis mellifera) mtDNA nomenclature using phylogeny" in Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic (2022):753,
https://hdl.handle.net/21.15107/rcub_ibiss_5035 .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Erić, Pavle
AU  - Stanisavljević, Ljubiša
AU  - Tanasković, Marija
PY  - 2022
UR  - https://eurbee9.bio.bg.ac.rs/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5038
AB  - The honey bee is one of the most economically and ecologically important species currently facing serious challenges. Its history is marked by strong anthropogenic influence and its local populations are rapidly changing while its diversity is constantly manipulated by beekeepers through the import of foreign queens, selection, and migratory beekeeping. The practice of queen importation caused one of the most disastrous events in the history of beekeeping when in the mid-20th century Varroa mite was accidentally imported to Europe. This infestation, combined with the degradation of natural habitats, was believed to have caused the disappearance of free-living populations throughout European forests. Recent studies challenge this view and demonstrate that honey bees are able to survive in nature without human interference.
To assess the genetic diversity of different honey bee colonies, we analyzed mitochondrial DNA and 14 microsatellite loci in samples collected from 102 apiaries (with 542 hives) and 55 free-living colonies (FC) from the urban and natural environments throughout Serbia.
Standard parameters of genetic diversity for both genetic markers showed that FC exhibit higher values compared to managed colonies (MC). Values of pairwise F ST genetic distances were higher when FC were compared to MC from different parts of Serbia while MC exhibited somewhat lower values when compared among themselves. Relatedness estimates demonstrated that feral colonies are more related among themselves than they are to other MC and vice versa, implying the existence of a viable and stable free-living population of honey bees. A significant change in the distribution and diversity of mtDNA lineages among MC compared to the historical data for Serbia was detected while genetic analysis based on microsatellite data demonstrated a high genetic uniformity.
Our data confirmed the existence of a genetically diverse and distinctive free-living population of honey bees in Serbia worthy of protection as well as a significant change in the genetic diversity of managed honey bees that calls for immediate change in the current beekeeping practices so that genetic diversity of locally adapted populations could be preserved.
PB  - European Association for Bee Research
C3  - Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia
T1  - Free-living vs. managed honey bees – a population genetic approach
SP  - 48
EP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5038
ER  - 

@conference{
author = "Davidović, Slobodan and Patenković, Aleksandra and Erić, Katarina and Erić, Pavle and Stanisavljević, Ljubiša and Tanasković, Marija",
year = "2022",
abstract = "The honey bee is one of the most economically and ecologically important species currently facing serious challenges. Its history is marked by strong anthropogenic influence and its local populations are rapidly changing while its diversity is constantly manipulated by beekeepers through the import of foreign queens, selection, and migratory beekeeping. The practice of queen importation caused one of the most disastrous events in the history of beekeeping when in the mid-20th century Varroa mite was accidentally imported to Europe. This infestation, combined with the degradation of natural habitats, was believed to have caused the disappearance of free-living populations throughout European forests. Recent studies challenge this view and demonstrate that honey bees are able to survive in nature without human interference.
To assess the genetic diversity of different honey bee colonies, we analyzed mitochondrial DNA and 14 microsatellite loci in samples collected from 102 apiaries (with 542 hives) and 55 free-living colonies (FC) from the urban and natural environments throughout Serbia.
Standard parameters of genetic diversity for both genetic markers showed that FC exhibit higher values compared to managed colonies (MC). Values of pairwise F ST genetic distances were higher when FC were compared to MC from different parts of Serbia while MC exhibited somewhat lower values when compared among themselves. Relatedness estimates demonstrated that feral colonies are more related among themselves than they are to other MC and vice versa, implying the existence of a viable and stable free-living population of honey bees. A significant change in the distribution and diversity of mtDNA lineages among MC compared to the historical data for Serbia was detected while genetic analysis based on microsatellite data demonstrated a high genetic uniformity.
Our data confirmed the existence of a genetically diverse and distinctive free-living population of honey bees in Serbia worthy of protection as well as a significant change in the genetic diversity of managed honey bees that calls for immediate change in the current beekeeping practices so that genetic diversity of locally adapted populations could be preserved.",
publisher = "European Association for Bee Research",
journal = "Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia",
title = "Free-living vs. managed honey bees – a population genetic approach",
pages = "48-49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5038"
}

Davidović, S., Patenković, A., Erić, K., Erić, P., Stanisavljević, L.,& Tanasković, M.. (2022). Free-living vs. managed honey bees – a population genetic approach. in Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia
European Association for Bee Research., 48-49.
https://hdl.handle.net/21.15107/rcub_ibiss_5038

Davidović S, Patenković A, Erić K, Erić P, Stanisavljević L, Tanasković M. Free-living vs. managed honey bees – a population genetic approach. in Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia. 2022;:48-49.
https://hdl.handle.net/21.15107/rcub_ibiss_5038 .

Davidović, Slobodan, Patenković, Aleksandra, Erić, Katarina, Erić, Pavle, Stanisavljević, Ljubiša, Tanasković, Marija, "Free-living vs. managed honey bees – a population genetic approach" in Abstract Book: EurBee 9: 9th European Congress of Apidology; 2022 Sep 20-22; Belgrade, Serbia (2022):48-49,
https://hdl.handle.net/21.15107/rcub_ibiss_5038 .

TY  - CONF
AU  - Davidović, Slobodan
AU  - Patenković, Aleksandra
AU  - Erić, Pavle
AU  - Erić, Katarina
AU  - Stanisavljević, Ljubiša
AU  - Tanasković, Marija
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5036
AB  - Zbog svoje uloge oprašivača kao i proizvoda koji se dobijaju od nje, medonosna pčela je prepoznata kao jedna od najvažnijih uzgajanih vrsta životinja. Trenutno je poznato 33 podvrsta koje su karakteristične za regione u kojima se uzgajaju. Za Srbiju su bile autohtone dve podvrste: Apis mellifera carnica i Apis mellifera macedonica. Meñutim, u poslednjih dvanaest godina došlo je do nagle ekspanzije pčelarstva koje je pratilo povećanje broja pčelara, košnica i broja selekcionih centara za uzgoj matica što je uslovilo značajne promene u genetičkoj raznovrsnosti ove važne vrste. Za potrebe molekularno genetičkih analiza, a radi utvrñivanja genetičke strukture populacije uzgajanih medonosnih pčela, uzorkovane su pčele radlilice iz 542 košnice poreklom iz 102 stacionarna pčelinjaka širom Srbije. Genetička raznovrsnost je procenjena analizom varijabilnosti mikrosatelitnih ponovaka iz 14 lokusa. Razlikovanje podvrste A. m. carnica od A. m. macedonica rañeno je na osnovu analize specifičnog polimorfizma u mitohondrijskoj DNK.
U sakupljenom uzorku nije detektovano prisustvo mtDNK linije karakteristične za podvrstu A. m. macedonica što ukazuje na gubitak jedne od autohtonih podvrsta. Vrednosti parametara genetičke raznovrsnosti su za većinu analiziranih regiona ujednačeni dok je očekivana heterozigotnost kod svih regiona viša od opažene. Najveća prosečna vrednost za raznovrsnost gena po lokusima zapažena je za Sjeničko-Pešterski region dok je najveći prosečni broj alela detektovan u okolini Leskovca. Mera genetičke udaljenosti, FST, izmeñu parova analiziranih populacija je pokazala da se pčele poreklom iz Sjeničko-Pešterskog regiona najviše razlikuju dok su pčele poreklom iz ostalih regiona meñusobno sličnije. Dodatno, diskriminativna analiza glavnih komponenti (DAPC) je pokazala visoku stopu preklapanja jedinki poreklom iz različitih regiona. Ovi podaci ukazuju na visok stepen protoka gena izmeñu različitih regiona Srbije koji su geografski udaljeni i imaju drugačije uslove životne sredine. Dobijeni rezultat je najverovatnije posledica savremenih pčelarskih praksi koje podrazumevaju migratorno pčelarstvo i kupovinu komercijlano dostupnih matica. Opisani način održavanja pčelinjaka dovodi do kontakta i protoka gena izmeñu pčela poreklom iz različitih regiona što nije slučaj u prirodnim uslovima ili prilikom tradicionalnog načina održavanja stacionarnih pčelinjaka uz praksu razrojavanja i samostalnu proizvodnju matica.
Sprovedene analize ukazuju na gubitak genetičke raznovrsnosti kod lokalno adaptiranih populacija kao i gubitak jedne od dve podvrste medonosnih pčela autohtonih na teritoriji Srbije. Trenutna situacija zahteva preispitivanje savremenih praksi koje se primenjuju u pčelarstvu i primenu adekvatnih mera kako bi se očuvala genetička raznovrsnost.
PB  - Belgrade : Entomological Society of Serbia
C3  - Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.
T1  - Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije
SP  - 47
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5036
ER  - 

@conference{
author = "Davidović, Slobodan and Patenković, Aleksandra and Erić, Pavle and Erić, Katarina and Stanisavljević, Ljubiša and Tanasković, Marija",
year = "2022",
abstract = "Zbog svoje uloge oprašivača kao i proizvoda koji se dobijaju od nje, medonosna pčela je prepoznata kao jedna od najvažnijih uzgajanih vrsta životinja. Trenutno je poznato 33 podvrsta koje su karakteristične za regione u kojima se uzgajaju. Za Srbiju su bile autohtone dve podvrste: Apis mellifera carnica i Apis mellifera macedonica. Meñutim, u poslednjih dvanaest godina došlo je do nagle ekspanzije pčelarstva koje je pratilo povećanje broja pčelara, košnica i broja selekcionih centara za uzgoj matica što je uslovilo značajne promene u genetičkoj raznovrsnosti ove važne vrste. Za potrebe molekularno genetičkih analiza, a radi utvrñivanja genetičke strukture populacije uzgajanih medonosnih pčela, uzorkovane su pčele radlilice iz 542 košnice poreklom iz 102 stacionarna pčelinjaka širom Srbije. Genetička raznovrsnost je procenjena analizom varijabilnosti mikrosatelitnih ponovaka iz 14 lokusa. Razlikovanje podvrste A. m. carnica od A. m. macedonica rañeno je na osnovu analize specifičnog polimorfizma u mitohondrijskoj DNK.
U sakupljenom uzorku nije detektovano prisustvo mtDNK linije karakteristične za podvrstu A. m. macedonica što ukazuje na gubitak jedne od autohtonih podvrsta. Vrednosti parametara genetičke raznovrsnosti su za većinu analiziranih regiona ujednačeni dok je očekivana heterozigotnost kod svih regiona viša od opažene. Najveća prosečna vrednost za raznovrsnost gena po lokusima zapažena je za Sjeničko-Pešterski region dok je najveći prosečni broj alela detektovan u okolini Leskovca. Mera genetičke udaljenosti, FST, izmeñu parova analiziranih populacija je pokazala da se pčele poreklom iz Sjeničko-Pešterskog regiona najviše razlikuju dok su pčele poreklom iz ostalih regiona meñusobno sličnije. Dodatno, diskriminativna analiza glavnih komponenti (DAPC) je pokazala visoku stopu preklapanja jedinki poreklom iz različitih regiona. Ovi podaci ukazuju na visok stepen protoka gena izmeñu različitih regiona Srbije koji su geografski udaljeni i imaju drugačije uslove životne sredine. Dobijeni rezultat je najverovatnije posledica savremenih pčelarskih praksi koje podrazumevaju migratorno pčelarstvo i kupovinu komercijlano dostupnih matica. Opisani način održavanja pčelinjaka dovodi do kontakta i protoka gena izmeñu pčela poreklom iz različitih regiona što nije slučaj u prirodnim uslovima ili prilikom tradicionalnog načina održavanja stacionarnih pčelinjaka uz praksu razrojavanja i samostalnu proizvodnju matica.
Sprovedene analize ukazuju na gubitak genetičke raznovrsnosti kod lokalno adaptiranih populacija kao i gubitak jedne od dve podvrste medonosnih pčela autohtonih na teritoriji Srbije. Trenutna situacija zahteva preispitivanje savremenih praksi koje se primenjuju u pčelarstvu i primenu adekvatnih mera kako bi se očuvala genetička raznovrsnost.",
publisher = "Belgrade : Entomological Society of Serbia",
journal = "Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.",
title = "Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije",
pages = "47-48",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5036"
}

Davidović, S., Patenković, A., Erić, P., Erić, K., Stanisavljević, L.,& Tanasković, M.. (2022). Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.
Belgrade : Entomological Society of Serbia., 47-48.
https://hdl.handle.net/21.15107/rcub_ibiss_5036

Davidović S, Patenković A, Erić P, Erić K, Stanisavljević L, Tanasković M. Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije. in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia.. 2022;:47-48.
https://hdl.handle.net/21.15107/rcub_ibiss_5036 .

Davidović, Slobodan, Patenković, Aleksandra, Erić, Pavle, Erić, Katarina, Stanisavljević, Ljubiša, Tanasković, Marija, "Antropogeni uticaj na genetičku raznovrsnost gajenih društava medonosne pčele (Apis mellifera) na teritoriji Srbije" in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia. (2022):47-48,
https://hdl.handle.net/21.15107/rcub_ibiss_5036 .

TY  - DATA
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Kusza, Szilvia
AU  - Oleksa, Andzrej
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4779
AB  - Figure S1. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 14 microsatellite loci (A7, A8, A14, A24, A28, A35, A43, A79, A88, A107, A113, Ap43, Ap249 and B124) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries and 8 different localities from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica) and South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia. In figure S1 the first three principal components (PC) are presented.
Figure S7. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 9 microsatellite loci (A7, A14, A24, A28, A35, A43, A88, A107 and A113) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries and 8 different localities from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica) and South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia and four different European countries (Spain, Italy, Poland and Hungary). In this figure four different A. mellifera species are presented as well: A. m. iberica from Spain, A. m. ligustica from Italy, A. m. mellifera from Poland, A. m. carnica from Hungary, Poland and Serbia. In figure S7 the first three principal components (PC) are presented.
T2  - Insects
T1  - Supplementary material "Further evidence of population admixture in the Serbian honey bee population"
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4779
ER  - 

@misc{
author = "Tanasković, Marija and Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Mihajlović, Milica and Tanasić, Vanja and Kusza, Szilvia and Oleksa, Andzrej and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2022",
abstract = "Figure S1. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 14 microsatellite loci (A7, A8, A14, A24, A28, A35, A43, A79, A88, A107, A113, Ap43, Ap249 and B124) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries and 8 different localities from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica) and South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia. In figure S1 the first three principal components (PC) are presented.
Figure S7. 3D plot demonstrating genetic variability analyzed using DAPC method based on the data from 9 microsatellite loci (A7, A14, A24, A28, A35, A43, A88, A107 and A113) for managed honey bees (Apis mellifera) colonies originating from stationary apiaries and 8 different localities from the North (Fruška gora, Deliblatska peščara, Vršac, Subotica) and South (Leskovac, Tromeđa, Vlasina, Stara planina) parts of Serbia and four different European countries (Spain, Italy, Poland and Hungary). In this figure four different A. mellifera species are presented as well: A. m. iberica from Spain, A. m. ligustica from Italy, A. m. mellifera from Poland, A. m. carnica from Hungary, Poland and Serbia. In figure S7 the first three principal components (PC) are presented.",
journal = "Insects",
title = "Supplementary material "Further evidence of population admixture in the Serbian honey bee population"",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4779"
}

Tanasković, M., Erić, P., Patenković, A., Erić, K., Mihajlović, M., Tanasić, V., Kusza, S., Oleksa, A., Stanisavljević, L.,& Davidović, S.. (2022). Supplementary material "Further evidence of population admixture in the Serbian honey bee population". in Insects.
https://hdl.handle.net/21.15107/rcub_ibiss_4779

Tanasković M, Erić P, Patenković A, Erić K, Mihajlović M, Tanasić V, Kusza S, Oleksa A, Stanisavljević L, Davidović S. Supplementary material "Further evidence of population admixture in the Serbian honey bee population". in Insects. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_4779 .

Tanasković, Marija, Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Mihajlović, Milica, Tanasić, Vanja, Kusza, Szilvia, Oleksa, Andzrej, Stanisavljević, Ljubiša, Davidović, Slobodan, "Supplementary material "Further evidence of population admixture in the Serbian honey bee population"" in Insects (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_4779 .

TY  - JOUR
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4429
AB  - Local populations of Apis mellifera are rapidly changing by modern beekeeping through the introduction of nonnative queens, selection and migratory beekeeping. To assess the genetic diversity of contemporary managed honey bees in Serbia, we sequenced mitochondrial tRNAleu-cox2 intergenic region of 241 worker bees from 46 apiaries at eight localities. Nine haplotypes were observed in our samples, with C2d being the most common and widespread. To evaluate genetic diversity patterns, we compared our data with 1696 sequences from the NCBI GenBank from neighbouring countries and Serbia. All 32 detected haplotypes belonged to the Southeast Europe lineage C, with two newly described haplotypes from our sample. The most frequent haplotype was C2d, followed by C2c and C1a. To distinguish A. m. carnica from A. m. macedonica, both previously reported in Serbia, PCR-RFLP analysis on the COI gene segment of mtDNA was used, and the result showed only the presence of A.m. carnica subspecies. An MDS plot constructed on pairwise Fst values showed significant geographical stratification. Our samples are grouped together, but distant from the Serbian dataset from the GenBank. This, with the absence of A. m. macedonica subspecies from its historic range of distribution in southern Serbia, indicates that honey bee populations are changing rapidly due to the anthropogenic influence.
PB  - Basel: MDPI
T2  - Insects
T1  - MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity
IS  - 9
VL  - 12
DO  - 10.3390/insects12090767
SP  - 767
ER  - 

@article{
author = "Tanasković, Marija and Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Mihajlović, Milica and Tanasić, Vanja and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2021",
abstract = "Local populations of Apis mellifera are rapidly changing by modern beekeeping through the introduction of nonnative queens, selection and migratory beekeeping. To assess the genetic diversity of contemporary managed honey bees in Serbia, we sequenced mitochondrial tRNAleu-cox2 intergenic region of 241 worker bees from 46 apiaries at eight localities. Nine haplotypes were observed in our samples, with C2d being the most common and widespread. To evaluate genetic diversity patterns, we compared our data with 1696 sequences from the NCBI GenBank from neighbouring countries and Serbia. All 32 detected haplotypes belonged to the Southeast Europe lineage C, with two newly described haplotypes from our sample. The most frequent haplotype was C2d, followed by C2c and C1a. To distinguish A. m. carnica from A. m. macedonica, both previously reported in Serbia, PCR-RFLP analysis on the COI gene segment of mtDNA was used, and the result showed only the presence of A.m. carnica subspecies. An MDS plot constructed on pairwise Fst values showed significant geographical stratification. Our samples are grouped together, but distant from the Serbian dataset from the GenBank. This, with the absence of A. m. macedonica subspecies from its historic range of distribution in southern Serbia, indicates that honey bee populations are changing rapidly due to the anthropogenic influence.",
publisher = "Basel: MDPI",
journal = "Insects",
title = "MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity",
number = "9",
volume = "12",
doi = "10.3390/insects12090767",
pages = "767"
}

Tanasković, M., Erić, P., Patenković, A., Erić, K., Mihajlović, M., Tanasić, V., Stanisavljević, L.,& Davidović, S.. (2021). MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity. in Insects
Basel: MDPI., 12(9), 767.
https://doi.org/10.3390/insects12090767

Tanasković M, Erić P, Patenković A, Erić K, Mihajlović M, Tanasić V, Stanisavljević L, Davidović S. MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity. in Insects. 2021;12(9):767.
doi:10.3390/insects12090767 .

Tanasković, Marija, Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Mihajlović, Milica, Tanasić, Vanja, Stanisavljević, Ljubiša, Davidović, Slobodan, "MtDNA Analysis Indicates Human-Induced Temporal Changes of Serbian Honey Bees Diversity" in Insects, 12, no. 9 (2021):767,
https://doi.org/10.3390/insects12090767 . .

TY  - JOUR
AU  - Kim, Bernard Y
AU  - Wang, Jeremy R
AU  - Miller, Danny E
AU  - Barmina, Olga
AU  - Delaney, Emily
AU  - Thompson, Ammon
AU  - Comeault, Aaron A
AU  - Peede, David
AU  - D'Agostino, Emmanuel RR
AU  - Pelaez, Julianne
AU  - Aguilar, Jessica M
AU  - Haji, Diler
AU  - Matsunaga, Teruyuki
AU  - Armstrong, Ellie E
AU  - Zych, Molly
AU  - Ogawa, Yoshitaka
AU  - Stamenković-Radak, Marina
AU  - Jelić, Mihailo
AU  - Savić Veselinović, Marija
AU  - Tanasković, Marija
AU  - Erić, Pavle
AU  - Gao, Jian-Jun
AU  - Katoh, Takehiro K
AU  - Toda, Masanori J
AU  - Watabe, Hideaki
AU  - Watada, Masayoshi
AU  - Davis, Jeremy S
AU  - Moyle, Leonie C
AU  - Manoli, Giulia
AU  - Bertolini, Enrico
AU  - Košťál, Vladimír
AU  - Hawley, R Scott
AU  - Takahashi, Aya
AU  - Jones, Corbin D
AU  - Price, Donald K
AU  - Whiteman, Noah
AU  - Kopp, Artyom
AU  - Matute, Daniel R
AU  - Petrov, Dmitri A
PY  - 2021
UR  - https://elifesciences.org/articles/66405
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8337076
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4457
AB  - Over 100 years of studies in Drosophila melanogaster and related species in the genus Drosophila have facilitated key discoveries in genetics, genomics, and evolution. While high-quality genome assemblies exist for several species in this group, they only encompass a small fraction of the genus. Recent advances in long-read sequencing allow high-quality genome assemblies for tens or even hundreds of species to be efficiently generated. Here, we utilize Oxford Nanopore sequencing to build an open community resource of genome assemblies for 101 lines of 93 drosophilid species encompassing 14 species groups and 35 sub-groups. The genomes are highly contiguous and complete, with an average contig N50 of 10.5 Mb and greater than 97% BUSCO completeness in 97/101 assemblies. We show that Nanopore-based assemblies are highly accurate in coding regions, particularly with respect to coding insertions and deletions. These assemblies, along with a detailed laboratory protocol and assembly pipelines, are released as a public resource and will serve as a starting point for addressing broad questions of genetics, ecology, and evolution at the scale of hundreds of species.
PB  - Cambridge: eLife Sciences Publications Ltd
T2  - eLife
T1  - Highly contiguous assemblies of 101 drosophilid genomes.
VL  - 10
DO  - 10.7554/eLife.66405
SP  - e66405
ER  - 

@article{
author = "Kim, Bernard Y and Wang, Jeremy R and Miller, Danny E and Barmina, Olga and Delaney, Emily and Thompson, Ammon and Comeault, Aaron A and Peede, David and D'Agostino, Emmanuel RR and Pelaez, Julianne and Aguilar, Jessica M and Haji, Diler and Matsunaga, Teruyuki and Armstrong, Ellie E and Zych, Molly and Ogawa, Yoshitaka and Stamenković-Radak, Marina and Jelić, Mihailo and Savić Veselinović, Marija and Tanasković, Marija and Erić, Pavle and Gao, Jian-Jun and Katoh, Takehiro K and Toda, Masanori J and Watabe, Hideaki and Watada, Masayoshi and Davis, Jeremy S and Moyle, Leonie C and Manoli, Giulia and Bertolini, Enrico and Košťál, Vladimír and Hawley, R Scott and Takahashi, Aya and Jones, Corbin D and Price, Donald K and Whiteman, Noah and Kopp, Artyom and Matute, Daniel R and Petrov, Dmitri A",
year = "2021",
abstract = "Over 100 years of studies in Drosophila melanogaster and related species in the genus Drosophila have facilitated key discoveries in genetics, genomics, and evolution. While high-quality genome assemblies exist for several species in this group, they only encompass a small fraction of the genus. Recent advances in long-read sequencing allow high-quality genome assemblies for tens or even hundreds of species to be efficiently generated. Here, we utilize Oxford Nanopore sequencing to build an open community resource of genome assemblies for 101 lines of 93 drosophilid species encompassing 14 species groups and 35 sub-groups. The genomes are highly contiguous and complete, with an average contig N50 of 10.5 Mb and greater than 97% BUSCO completeness in 97/101 assemblies. We show that Nanopore-based assemblies are highly accurate in coding regions, particularly with respect to coding insertions and deletions. These assemblies, along with a detailed laboratory protocol and assembly pipelines, are released as a public resource and will serve as a starting point for addressing broad questions of genetics, ecology, and evolution at the scale of hundreds of species.",
publisher = "Cambridge: eLife Sciences Publications Ltd",
journal = "eLife",
title = "Highly contiguous assemblies of 101 drosophilid genomes.",
volume = "10",
doi = "10.7554/eLife.66405",
pages = "e66405"
}

Kim, B. Y., Wang, J. R., Miller, D. E., Barmina, O., Delaney, E., Thompson, A., Comeault, A. A., Peede, D., D'Agostino, E. R., Pelaez, J., Aguilar, J. M., Haji, D., Matsunaga, T., Armstrong, E. E., Zych, M., Ogawa, Y., Stamenković-Radak, M., Jelić, M., Savić Veselinović, M., Tanasković, M., Erić, P., Gao, J., Katoh, T. K., Toda, M. J., Watabe, H., Watada, M., Davis, J. S., Moyle, L. C., Manoli, G., Bertolini, E., Košťál, V., Hawley, R. S., Takahashi, A., Jones, C. D., Price, D. K., Whiteman, N., Kopp, A., Matute, D. R.,& Petrov, D. A.. (2021). Highly contiguous assemblies of 101 drosophilid genomes.. in eLife
Cambridge: eLife Sciences Publications Ltd., 10, e66405.
https://doi.org/10.7554/eLife.66405

Kim BY, Wang JR, Miller DE, Barmina O, Delaney E, Thompson A, Comeault AA, Peede D, D'Agostino ER, Pelaez J, Aguilar JM, Haji D, Matsunaga T, Armstrong EE, Zych M, Ogawa Y, Stamenković-Radak M, Jelić M, Savić Veselinović M, Tanasković M, Erić P, Gao J, Katoh TK, Toda MJ, Watabe H, Watada M, Davis JS, Moyle LC, Manoli G, Bertolini E, Košťál V, Hawley RS, Takahashi A, Jones CD, Price DK, Whiteman N, Kopp A, Matute DR, Petrov DA. Highly contiguous assemblies of 101 drosophilid genomes.. in eLife. 2021;10:e66405.
doi:10.7554/eLife.66405 .

Kim, Bernard Y, Wang, Jeremy R, Miller, Danny E, Barmina, Olga, Delaney, Emily, Thompson, Ammon, Comeault, Aaron A, Peede, David, D'Agostino, Emmanuel RR, Pelaez, Julianne, Aguilar, Jessica M, Haji, Diler, Matsunaga, Teruyuki, Armstrong, Ellie E, Zych, Molly, Ogawa, Yoshitaka, Stamenković-Radak, Marina, Jelić, Mihailo, Savić Veselinović, Marija, Tanasković, Marija, Erić, Pavle, Gao, Jian-Jun, Katoh, Takehiro K, Toda, Masanori J, Watabe, Hideaki, Watada, Masayoshi, Davis, Jeremy S, Moyle, Leonie C, Manoli, Giulia, Bertolini, Enrico, Košťál, Vladimír, Hawley, R Scott, Takahashi, Aya, Jones, Corbin D, Price, Donald K, Whiteman, Noah, Kopp, Artyom, Matute, Daniel R, Petrov, Dmitri A, "Highly contiguous assemblies of 101 drosophilid genomes." in eLife, 10 (2021):e66405,
https://doi.org/10.7554/eLife.66405 . .

TY  - CONF
AU  - Erić, Katarina
AU  - Erić, Pavle
AU  - Davidović, Slobodan
AU  - Savić Veselinović, Marija
AU  - Patenković, Aleksandra
AU  - Stamenković-Radak, Marina
AU  - Tanasković, Marija
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4924
AB  - Climate change and global warming are affecting many insect species in different ways.
Organisms develop diverse mechanisms responding to variable environmental conditions. Due to
global warming and the rise in mean and extreme temperatures, the importance of an individual’s
ability to adapt to temperature stress will further increase. Our focus in this study is the thermal
response, which is considered to be one of the crucial elements of individual fitness and survival in
a fast changing environment. We investigated stress resistance traits: desiccation and heat knock-
down time in two Drospohila subobscura populations from two different altitudes (1080m and 1580m
a.s.l.) along Stara planina mountain slopes in Serbia. The F1 progeny of the mass populations from
both localities was used to establish six experimental groups at three different temperature regimes:
25⁰C, 19⁰C and 16⁰C. Our aim was to determine whether there is a correlation between
altitude/origin of populations and/or laboratory thermal evolution within these resistance traits.
Flies from all groups were tested (scored every hour) for desiccation mortality at those temperatures.
To score heat knock-down time all groups were placed into vials with moistened cotton plugs to
prevent desiccation, kept at 37⁰C for seven hours, and knock down flies were scored every 30
minutes. Our results indicate that the population origin, as well as laboratory thermal evolution
have a significant influence on the analysed traits. Individuals originating from the higher altitudes
reared at higher temperatures show better resistance to thermal shock.
PB  - Basel: MDPI
C3  - Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
T1  - Thermal Adaptation and Stress Resistance in D. subobscura Populations from Two Altitudes at Stara Planina Mountain (Serbia)
DO  - 10.3390/IECE-10532
SP  - 10532
ER  - 

@conference{
author = "Erić, Katarina and Erić, Pavle and Davidović, Slobodan and Savić Veselinović, Marija and Patenković, Aleksandra and Stamenković-Radak, Marina and Tanasković, Marija",
year = "2021",
abstract = "Climate change and global warming are affecting many insect species in different ways.
Organisms develop diverse mechanisms responding to variable environmental conditions. Due to
global warming and the rise in mean and extreme temperatures, the importance of an individual’s
ability to adapt to temperature stress will further increase. Our focus in this study is the thermal
response, which is considered to be one of the crucial elements of individual fitness and survival in
a fast changing environment. We investigated stress resistance traits: desiccation and heat knock-
down time in two Drospohila subobscura populations from two different altitudes (1080m and 1580m
a.s.l.) along Stara planina mountain slopes in Serbia. The F1 progeny of the mass populations from
both localities was used to establish six experimental groups at three different temperature regimes:
25⁰C, 19⁰C and 16⁰C. Our aim was to determine whether there is a correlation between
altitude/origin of populations and/or laboratory thermal evolution within these resistance traits.
Flies from all groups were tested (scored every hour) for desiccation mortality at those temperatures.
To score heat knock-down time all groups were placed into vials with moistened cotton plugs to
prevent desiccation, kept at 37⁰C for seven hours, and knock down flies were scored every 30
minutes. Our results indicate that the population origin, as well as laboratory thermal evolution
have a significant influence on the analysed traits. Individuals originating from the higher altitudes
reared at higher temperatures show better resistance to thermal shock.",
publisher = "Basel: MDPI",
journal = "Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online",
title = "Thermal Adaptation and Stress Resistance in D. subobscura Populations from Two Altitudes at Stara Planina Mountain (Serbia)",
doi = "10.3390/IECE-10532",
pages = "10532"
}

Erić, K., Erić, P., Davidović, S., Savić Veselinović, M., Patenković, A., Stamenković-Radak, M.,& Tanasković, M.. (2021). Thermal Adaptation and Stress Resistance in D. subobscura Populations from Two Altitudes at Stara Planina Mountain (Serbia). in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
Basel: MDPI., 10532.
https://doi.org/10.3390/IECE-10532

Erić K, Erić P, Davidović S, Savić Veselinović M, Patenković A, Stamenković-Radak M, Tanasković M. Thermal Adaptation and Stress Resistance in D. subobscura Populations from Two Altitudes at Stara Planina Mountain (Serbia). in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online. 2021;:10532.
doi:10.3390/IECE-10532 .

Erić, Katarina, Erić, Pavle, Davidović, Slobodan, Savić Veselinović, Marija, Patenković, Aleksandra, Stamenković-Radak, Marina, Tanasković, Marija, "Thermal Adaptation and Stress Resistance in D. subobscura Populations from Two Altitudes at Stara Planina Mountain (Serbia)" in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online (2021):10532,
https://doi.org/10.3390/IECE-10532 . .

TY  - CONF
AU  - Rakić, Mina
AU  - Jelić, Mihailo
AU  - Stamenković-Radak, Marina
AU  - Patenković, Aleksandra
AU  - Tanasković, Marija
AU  - Erić, Katarina
AU  - Davidović, Slobodan
AU  - Erić, Pavle
AU  - Savić Veselinović, Marija
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4923
AB  - Heavy metals are one of the most persistent pollutants that are introduced into the environment through human activity and ever so growing industrialization, exploitation and utilization of natural heavy metal deposits. Due to their toxicity, heavy metals have severely negative effects on organisms, mainly through oxidative stress. The objective of this research was to investigate the influence of heavy metals on the expression of six metallothionein genes (Mtn A, B, C, D, E, F), with the intention to show to which extent the concentration of metals and the duration of the exposure influence Mtn gene expression, as well as the potential correlation of certain metallothioneins to a specific metal or combination of them. Larvae of the fruit fly Drosophila subobscura were subjected to the treatments with four heavy metals: zinc, copper, cadmium or lead. A combination of cadmium and lead was also included, to detect the combinatory effect of these metals. Treatment with each of the heavy metals was conducted in two concentrations, in short-term (24 hours) and long-term (10 days) time frame. Gene expression was analyzed with qPCR. The pattern of gene expression in response to various treatments indicates that some genes show specificity to certain metals, while others demonstrate a broader response or no response at all.
PB  - Basel: MDPI
C3  - Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
T1  - Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures
DO  - 10.3390/IECE-10508
SP  - 10508
ER  - 

@conference{
author = "Rakić, Mina and Jelić, Mihailo and Stamenković-Radak, Marina and Patenković, Aleksandra and Tanasković, Marija and Erić, Katarina and Davidović, Slobodan and Erić, Pavle and Savić Veselinović, Marija",
year = "2021",
abstract = "Heavy metals are one of the most persistent pollutants that are introduced into the environment through human activity and ever so growing industrialization, exploitation and utilization of natural heavy metal deposits. Due to their toxicity, heavy metals have severely negative effects on organisms, mainly through oxidative stress. The objective of this research was to investigate the influence of heavy metals on the expression of six metallothionein genes (Mtn A, B, C, D, E, F), with the intention to show to which extent the concentration of metals and the duration of the exposure influence Mtn gene expression, as well as the potential correlation of certain metallothioneins to a specific metal or combination of them. Larvae of the fruit fly Drosophila subobscura were subjected to the treatments with four heavy metals: zinc, copper, cadmium or lead. A combination of cadmium and lead was also included, to detect the combinatory effect of these metals. Treatment with each of the heavy metals was conducted in two concentrations, in short-term (24 hours) and long-term (10 days) time frame. Gene expression was analyzed with qPCR. The pattern of gene expression in response to various treatments indicates that some genes show specificity to certain metals, while others demonstrate a broader response or no response at all.",
publisher = "Basel: MDPI",
journal = "Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online",
title = "Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures",
doi = "10.3390/IECE-10508",
pages = "10508"
}

Rakić, M., Jelić, M., Stamenković-Radak, M., Patenković, A., Tanasković, M., Erić, K., Davidović, S., Erić, P.,& Savić Veselinović, M.. (2021). Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures. in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
Basel: MDPI., 10508.
https://doi.org/10.3390/IECE-10508

Rakić M, Jelić M, Stamenković-Radak M, Patenković A, Tanasković M, Erić K, Davidović S, Erić P, Savić Veselinović M. Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures. in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online. 2021;:10508.
doi:10.3390/IECE-10508 .

Rakić, Mina, Jelić, Mihailo, Stamenković-Radak, Marina, Patenković, Aleksandra, Tanasković, Marija, Erić, Katarina, Davidović, Slobodan, Erić, Pavle, Savić Veselinović, Marija, "Metallothionein Gene Expression in Drosophila subobscura Indicates Their Different Function in Response to Heavy Metal Exposures" in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online (2021):10508,
https://doi.org/10.3390/IECE-10508 . .

TY  - CONF
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Tanasković, Marija
AU  - Davidović, Slobodan
AU  - Rakić, Mina
AU  - Savić Veselinović, Marija
AU  - Stamenković-Radak, Marina
AU  - Jelić, Mihailo
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4894
AB  - Drosophila obscura is a very common fruit fly inhabiting European forests. This species has
a large number of mitochondrial haplotypes of Cyt b gene. We used experimental lines of D. obscura
to test the adaptive significance of intra -population variability of the mitochondrial genome
(mtDNA) and selective forces that maintain it. We chose three isofemale lines with distinct mitochondrial haplotypes of Cyt b gene from each of the two populations sampled in Serbia. Using backcrossing, we created nine experimental lines for each population with all combinations of mtDNA
haplotypes and nuclear genetic backgrounds (nuDNA). Individuals of both sexes were tested separately for desiccation resistance at two temperatures. Cox proportional hazards model, with four
factors: mtDNA, nuDNA, sex and temperature was used to analyze the survival data. In some comparisons we noticed significant effect of mtDNA on desiccation resistance, while all of them showed
significant effect of interaction between mitochondrial and nuclear genome. Temperature in interaction with mtDNA or mito-nuclear genotype more frequently showed significant effect on desiccation resistance compared to sex in interaction with mtDNA or mito-nuclear genotype. Our results
show adaptive significance of intra-population variation of mtDNA and importance of interactions
between mitochondrial and nuclear genome on fitness. Temperature specific mito-nuclear interaction rather than sex-specific selection on mito-nuclear genotypes maintains mtDNA variability in
this model species.
PB  - Basel: MDPI
C3  - Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
T1  - Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura
DO  - 10.3390/IECE-10522
SP  - 10522
ER  - 

@conference{
author = "Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Tanasković, Marija and Davidović, Slobodan and Rakić, Mina and Savić Veselinović, Marija and Stamenković-Radak, Marina and Jelić, Mihailo",
year = "2021",
abstract = "Drosophila obscura is a very common fruit fly inhabiting European forests. This species has
a large number of mitochondrial haplotypes of Cyt b gene. We used experimental lines of D. obscura
to test the adaptive significance of intra -population variability of the mitochondrial genome
(mtDNA) and selective forces that maintain it. We chose three isofemale lines with distinct mitochondrial haplotypes of Cyt b gene from each of the two populations sampled in Serbia. Using backcrossing, we created nine experimental lines for each population with all combinations of mtDNA
haplotypes and nuclear genetic backgrounds (nuDNA). Individuals of both sexes were tested separately for desiccation resistance at two temperatures. Cox proportional hazards model, with four
factors: mtDNA, nuDNA, sex and temperature was used to analyze the survival data. In some comparisons we noticed significant effect of mtDNA on desiccation resistance, while all of them showed
significant effect of interaction between mitochondrial and nuclear genome. Temperature in interaction with mtDNA or mito-nuclear genotype more frequently showed significant effect on desiccation resistance compared to sex in interaction with mtDNA or mito-nuclear genotype. Our results
show adaptive significance of intra-population variation of mtDNA and importance of interactions
between mitochondrial and nuclear genome on fitness. Temperature specific mito-nuclear interaction rather than sex-specific selection on mito-nuclear genotypes maintains mtDNA variability in
this model species.",
publisher = "Basel: MDPI",
journal = "Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online",
title = "Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura",
doi = "10.3390/IECE-10522",
pages = "10522"
}

Erić, P., Patenković, A., Erić, K., Tanasković, M., Davidović, S., Rakić, M., Savić Veselinović, M., Stamenković-Radak, M.,& Jelić, M.. (2021). Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura. in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online
Basel: MDPI., 10522.
https://doi.org/10.3390/IECE-10522

Erić P, Patenković A, Erić K, Tanasković M, Davidović S, Rakić M, Savić Veselinović M, Stamenković-Radak M, Jelić M. Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura. in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online. 2021;:10522.
doi:10.3390/IECE-10522 .

Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Tanasković, Marija, Davidović, Slobodan, Rakić, Mina, Savić Veselinović, Marija, Stamenković-Radak, Marina, Jelić, Mihailo, "Unraveling the adaptive significance of mitochondrial genome variability of Drosophila obscura" in Proceedings of the 1st International Electronic Conference on Entomology; 2021 Jul 1-15; Online (2021):10522,
https://doi.org/10.3390/IECE-10522 . .

TY  - CONF
AU  - Tanasković, Marija
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Erić, Pavle
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2021
UR  - https://sciforum.net/paper/view/10720
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4469
AB  - Practice of commercial honey bee breeding and selection for desired traits, intensification of queen importation and migration of once stationary apiaries significantly influences distribution and genetic diversity of local subspecies, populations and ecotypes. Bee colonies worldwide are facing serious declines resulting in colonies loss and reduction of genetic diversity. Thus, reassessing the genetic status of native honey bee populations becomes imperative. The latest reports, which include samples from nine years ago, suggest the presence of both Apis mellifera carnica in north and A. m. macedonica in the south of Serbia and significant hybridization between two subspecies.
To assess genetic diversity of contemporary managed honey bee colonies we used 14 microsatellite loci and analyzed 227 worker bees from 46 apiaries in 8 localities from northern and southern Serbia. RFLP analysis on the COI gene segment of mtDNA was used to distinguish A. m. carnica from A. m. macedonica. Mean number of alleles ranged from 5.14 to 9.00, observed heterozygosity from 0.43 to 0.56 and STRUCTURE analysis showed existence of three distinct genetic clusters. DAPC analysis showed huge overlapping of individuals from different parts of Serbia with weak clustering according to geographical origin of three groups. RFLP analysis showed the presence of A. m. carnica subspecies only.
Absence of A. m. macedonica subspecies from its historic range of distribution in southern Serbia as well as lack of distinctive geographical clusters suggest that selective breeding, queen import and migratory beekeeping practices strongly influenced genetic structure and diversity of honey bees leading to the genetic uniformisation and absence of locally adapted populations.
PB  - Basel: MDPI
C3  - The 1st International Electronic Conference on Entomology session Apiculture and Pollinators; 2021 Jul 1-15
T1  - Microsatellite Analysis of Apis mellifera from Northern and Southern Parts of Serbia
DO  - 10.3390/IECE-10720
ER  - 

@conference{
author = "Tanasković, Marija and Patenković, Aleksandra and Erić, Katarina and Erić, Pavle and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2021",
abstract = "Practice of commercial honey bee breeding and selection for desired traits, intensification of queen importation and migration of once stationary apiaries significantly influences distribution and genetic diversity of local subspecies, populations and ecotypes. Bee colonies worldwide are facing serious declines resulting in colonies loss and reduction of genetic diversity. Thus, reassessing the genetic status of native honey bee populations becomes imperative. The latest reports, which include samples from nine years ago, suggest the presence of both Apis mellifera carnica in north and A. m. macedonica in the south of Serbia and significant hybridization between two subspecies.
To assess genetic diversity of contemporary managed honey bee colonies we used 14 microsatellite loci and analyzed 227 worker bees from 46 apiaries in 8 localities from northern and southern Serbia. RFLP analysis on the COI gene segment of mtDNA was used to distinguish A. m. carnica from A. m. macedonica. Mean number of alleles ranged from 5.14 to 9.00, observed heterozygosity from 0.43 to 0.56 and STRUCTURE analysis showed existence of three distinct genetic clusters. DAPC analysis showed huge overlapping of individuals from different parts of Serbia with weak clustering according to geographical origin of three groups. RFLP analysis showed the presence of A. m. carnica subspecies only.
Absence of A. m. macedonica subspecies from its historic range of distribution in southern Serbia as well as lack of distinctive geographical clusters suggest that selective breeding, queen import and migratory beekeeping practices strongly influenced genetic structure and diversity of honey bees leading to the genetic uniformisation and absence of locally adapted populations.",
publisher = "Basel: MDPI",
journal = "The 1st International Electronic Conference on Entomology session Apiculture and Pollinators; 2021 Jul 1-15",
title = "Microsatellite Analysis of Apis mellifera from Northern and Southern Parts of Serbia",
doi = "10.3390/IECE-10720"
}

Tanasković, M., Patenković, A., Erić, K., Erić, P., Stanisavljević, L.,& Davidović, S.. (2021). Microsatellite Analysis of Apis mellifera from Northern and Southern Parts of Serbia. in The 1st International Electronic Conference on Entomology session Apiculture and Pollinators; 2021 Jul 1-15
Basel: MDPI..
https://doi.org/10.3390/IECE-10720

Tanasković M, Patenković A, Erić K, Erić P, Stanisavljević L, Davidović S. Microsatellite Analysis of Apis mellifera from Northern and Southern Parts of Serbia. in The 1st International Electronic Conference on Entomology session Apiculture and Pollinators; 2021 Jul 1-15. 2021;.
doi:10.3390/IECE-10720 .

Tanasković, Marija, Patenković, Aleksandra, Erić, Katarina, Erić, Pavle, Stanisavljević, Ljubiša, Davidović, Slobodan, "Microsatellite Analysis of Apis mellifera from Northern and Southern Parts of Serbia" in The 1st International Electronic Conference on Entomology session Apiculture and Pollinators; 2021 Jul 1-15 (2021),
https://doi.org/10.3390/IECE-10720 . .

TY  - CONF
AU  - Erić, Pavle
AU  - Patenković, Aleksandra
AU  - Erić, Katarina
AU  - Tanasić, Vanja
AU  - Mihajlović, Milica
AU  - Tanasković, Marija
AU  - Stanisavljević, Ljubiša
AU  - Davidović, Slobodan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4253
AB  - Abstract
Local populations of Apis mellifera are rapidly changing, with the gene pool of autochthonous breeds being depleted by beekeepers through the import of foreign queens not adapted to the local environment. To study their genetic structure and phylogenetic relationships, we gathered a large dataset from the Balkans and surrounding countries.
Our sample consisted of 246 honeybee colonies collected from 47 apiaries and 24 feral colonies divided into four subpopulations from southern Serbia, five from Vojvodina, and two from Belgrade. To evaluate genetic diversity patterns, we sequenced the mitochondrial tRNAleu-cox2 intergenic region. We compared our data to other published data on A.mellifera COI-COII intergenic region variability in the Balkans and neighboring countries. We pulled 1512 sequences from the NCBI GenBank, originating from 15 different populations. The 1782 mitochondrial sequences were grouped into 31 haplotypes, with two newly described haplotypes from our sample. All haplotypes belonged to the eastern Mediterranean C lineage. The most frequent haplotype was C2d, characteristic for A.m.macedonica, followed by C2c and C1a characteristic for A.m.carnica and A.m.ligustica respectively. In our samples 9 haplotypes were observed, with the C2d being the most common and widespread as it was detected in all 11 groups, followed by C2e that was detected in all but one group. C2c and C1a were a little less common than in the total sample but very widespread as they were present in seven groups.
When the Macedonia, Ukraine, and Belgrade honeybee populations which consisted of a single haplotype, were excluded, the haplotype diversity ranged from 0.0998 to 0.7477, nucleotide diversity ranged from the lowest value of 0.000114 to 0.003731. The mean number of pairwise differences for populations that had more than one haplotype ranged from 0.060577 to 2. MDS plot constructed on pairwise FST values shows significant geographical stratification, with our subpopulations being grouped together. Vojvodina being placed closer to Romania and Hungary datasets, while southern Serbia is closer to Bulgaria and Montenegro. Interestingly, our samples are not closely grouped with the Serbian dataset from the GenBank which indicates that honeybee populations are changing rapidly.
PB  - Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
C3  - Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia
T1  - Large scale mitochondrial DNA analysis of European Honey bee (Apis mellifera) populations from the Balkans, population genetics and phylogeographic perspective
IS  - 1
VL  - 43
SP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4253
ER  - 

@conference{
author = "Erić, Pavle and Patenković, Aleksandra and Erić, Katarina and Tanasić, Vanja and Mihajlović, Milica and Tanasković, Marija and Stanisavljević, Ljubiša and Davidović, Slobodan",
year = "2021",
abstract = "Abstract
Local populations of Apis mellifera are rapidly changing, with the gene pool of autochthonous breeds being depleted by beekeepers through the import of foreign queens not adapted to the local environment. To study their genetic structure and phylogenetic relationships, we gathered a large dataset from the Balkans and surrounding countries.
Our sample consisted of 246 honeybee colonies collected from 47 apiaries and 24 feral colonies divided into four subpopulations from southern Serbia, five from Vojvodina, and two from Belgrade. To evaluate genetic diversity patterns, we sequenced the mitochondrial tRNAleu-cox2 intergenic region. We compared our data to other published data on A.mellifera COI-COII intergenic region variability in the Balkans and neighboring countries. We pulled 1512 sequences from the NCBI GenBank, originating from 15 different populations. The 1782 mitochondrial sequences were grouped into 31 haplotypes, with two newly described haplotypes from our sample. All haplotypes belonged to the eastern Mediterranean C lineage. The most frequent haplotype was C2d, characteristic for A.m.macedonica, followed by C2c and C1a characteristic for A.m.carnica and A.m.ligustica respectively. In our samples 9 haplotypes were observed, with the C2d being the most common and widespread as it was detected in all 11 groups, followed by C2e that was detected in all but one group. C2c and C1a were a little less common than in the total sample but very widespread as they were present in seven groups.
When the Macedonia, Ukraine, and Belgrade honeybee populations which consisted of a single haplotype, were excluded, the haplotype diversity ranged from 0.0998 to 0.7477, nucleotide diversity ranged from the lowest value of 0.000114 to 0.003731. The mean number of pairwise differences for populations that had more than one haplotype ranged from 0.060577 to 2. MDS plot constructed on pairwise FST values shows significant geographical stratification, with our subpopulations being grouped together. Vojvodina being placed closer to Romania and Hungary datasets, while southern Serbia is closer to Bulgaria and Montenegro. Interestingly, our samples are not closely grouped with the Serbian dataset from the GenBank which indicates that honeybee populations are changing rapidly.",
publisher = "Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad",
journal = "Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia",
title = "Large scale mitochondrial DNA analysis of European Honey bee (Apis mellifera) populations from the Balkans, population genetics and phylogeographic perspective",
number = "1",
volume = "43",
pages = "116",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4253"
}

Erić, P., Patenković, A., Erić, K., Tanasić, V., Mihajlović, M., Tanasković, M., Stanisavljević, L.,& Davidović, S.. (2021). Large scale mitochondrial DNA analysis of European Honey bee (Apis mellifera) populations from the Balkans, population genetics and phylogeographic perspective. in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia
Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad., 43(1), 116.
https://hdl.handle.net/21.15107/rcub_ibiss_4253

Erić P, Patenković A, Erić K, Tanasić V, Mihajlović M, Tanasković M, Stanisavljević L, Davidović S. Large scale mitochondrial DNA analysis of European Honey bee (Apis mellifera) populations from the Balkans, population genetics and phylogeographic perspective. in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia. 2021;43(1):116.
https://hdl.handle.net/21.15107/rcub_ibiss_4253 .

Erić, Pavle, Patenković, Aleksandra, Erić, Katarina, Tanasić, Vanja, Mihajlović, Milica, Tanasković, Marija, Stanisavljević, Ljubiša, Davidović, Slobodan, "Large scale mitochondrial DNA analysis of European Honey bee (Apis mellifera) populations from the Balkans, population genetics and phylogeographic perspective" in Belgrade BioInformatics Conference 2021: Book of Abstracts; 2021 Jun 21-25; Vinča, Serbia, 43, no. 1 (2021):116,
https://hdl.handle.net/21.15107/rcub_ibiss_4253 .

TY  - JOUR
AU  - Erić, Pavle
AU  - Jelić, Mihailo
AU  - Savić-Veselinović, Marija
AU  - Kenig, Bojan
AU  - Anđelković, Marko
AU  - Stamenković-Radak, Marina
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0534-00121901213E
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3369
AB  - Mitochondrial DNA variability of Drosophila subobscura Collin from Southeastern Serbia was studied with respect to Restriction Site Analysis (RSA) of complete mitochondrial genome and the nucleotide sequence of Cytochrome b (Cyt b) gene. The aim was to shed more light on the evolutionary forces that shape mtDNA variation of this species. Samples were collected from two sites in the foothills of the Balkan Mountains. No genetic differentiation was found between groups and most of the variation was observed within them. Restriction analysis revealed two main haplotypes and several rare ones. The sequencing of Cyt b gene showed larger number of haplotypes, among which, one is being the most common. The majority of singletons differed from the most frequent haplotype by one nucleotide change. Although some of the observed nucleotide differences may affect their host’s fitness, the observed pattern of variation is consistent with the seasonal fluctuations in population size.
T2  - Genetika
T2  - Genetika
T1  - Nucleotide diversity of Cyt b gene in drosophila subobscura Collin
IS  - 1
VL  - 51
DO  - 10.2298/GENSR1901213E
SP  - 213
EP  - 226
ER  - 

@article{
author = "Erić, Pavle and Jelić, Mihailo and Savić-Veselinović, Marija and Kenig, Bojan and Anđelković, Marko and Stamenković-Radak, Marina",
year = "2019",
abstract = "Mitochondrial DNA variability of Drosophila subobscura Collin from Southeastern Serbia was studied with respect to Restriction Site Analysis (RSA) of complete mitochondrial genome and the nucleotide sequence of Cytochrome b (Cyt b) gene. The aim was to shed more light on the evolutionary forces that shape mtDNA variation of this species. Samples were collected from two sites in the foothills of the Balkan Mountains. No genetic differentiation was found between groups and most of the variation was observed within them. Restriction analysis revealed two main haplotypes and several rare ones. The sequencing of Cyt b gene showed larger number of haplotypes, among which, one is being the most common. The majority of singletons differed from the most frequent haplotype by one nucleotide change. Although some of the observed nucleotide differences may affect their host’s fitness, the observed pattern of variation is consistent with the seasonal fluctuations in population size.",
journal = "Genetika, Genetika",
title = "Nucleotide diversity of Cyt b gene in drosophila subobscura Collin",
number = "1",
volume = "51",
doi = "10.2298/GENSR1901213E",
pages = "213-226"
}

Erić, P., Jelić, M., Savić-Veselinović, M., Kenig, B., Anđelković, M.,& Stamenković-Radak, M.. (2019). Nucleotide diversity of Cyt b gene in drosophila subobscura Collin. in Genetika, 51(1), 213-226.
https://doi.org/10.2298/GENSR1901213E

Erić P, Jelić M, Savić-Veselinović M, Kenig B, Anđelković M, Stamenković-Radak M. Nucleotide diversity of Cyt b gene in drosophila subobscura Collin. in Genetika. 2019;51(1):213-226.
doi:10.2298/GENSR1901213E .

Erić, Pavle, Jelić, Mihailo, Savić-Veselinović, Marija, Kenig, Bojan, Anđelković, Marko, Stamenković-Radak, Marina, "Nucleotide diversity of Cyt b gene in drosophila subobscura Collin" in Genetika, 51, no. 1 (2019):213-226,
https://doi.org/10.2298/GENSR1901213E . .