TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Stanković, Saša S
AU  - Ćurčić, Nina B.
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5818
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.
VL  - 11
DO  - 10.3389/fevo.2023.1148309
SP  - 1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag and Todosijević, Marina and Stanković, Saša S and Ćurčić, Nina B. and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.",
volume = "11",
doi = "10.3389/fevo.2023.1148309",
pages = "1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L., Todosijević, M., Stanković, S. S., Ćurčić, N. B., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić L, Todosijević M, Stanković SS, Ćurčić NB, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag, Todosijević, Marina, Stanković, Saša S, Ćurčić, Nina B., Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities." in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Pavković-Lučić, Sofija
AU  - Trajković, Jelena
AU  - Miličić, Dragana
AU  - Anđelković, Boban
AU  - Lučić, Luka
AU  - Savić, Tatjana
AU  - Vujisić, Ljubodrag
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/arch.21866
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4776
AB  - In the world of complex smells in natural environment, feeding and mating represent two important olfactory-guided behaviors in Drosophila melanogaster (Diptera: Drosophilidae). Diet affects the chemoprofile composition of the individuals, which, indirectly, may significantly affect their mating success. In this study, chemoprofiles of recently mated flies belonging to four D. melanogaster strains, which were fed for many generations on different substrates (standard cornmeal-S strain; banana-B strain; carrot-C strain; tomato-T strain) were identified and quantified. In total, 67 chemical compounds were identified: 48 compounds were extracted from males maintained on banana and carrot, and 47 compounds from males maintained on cornmeal and tomato substrates, while total of 60 compounds were identified in females from all strains. The strains and the sexes significantly differed in qualitative nature of their chemoprofiles after mating. Significant differences in the relative amount of three major male pheromones (cis-vaccenyl acetate-cVA, (Z)-7-pentacosene, and (Z)-7-tricosene) and in female pheromone (Z,Z)-7,11-nonacosadiene among strains were also recorded. Furthermore, multivariate analysis of variance (MANOVA) pointed to significant differences between virgin and mated individuals of all strains and within both sexes. Differences in some of the well known sex pheromones were also identified when comparing their relative amount before and after mating. The presence of typical male pheromones in females, and vice versa may indicate their bidirectional transfer during copulation. Our results confirm significant effect of mating status on cuticular hydrocarbon (CHC) phenotypes in differently fed D. melanogaster flies.
PB  - Hoboken: John Wiley and Sons Inc.
T2  - Archives of Insect Biochemistry and Physiology
T2  - Archives of Insect Biochemistry and Physiology
T1  - "Scent of a fruit fly": Cuticular chemoprofiles after mating in differently fed Drosophila melanogaster (Diptera: Drosophilidae) strains.
DO  - 10.1002/arch.21866
SP  - e21866
ER  - 

@article{
author = "Pavković-Lučić, Sofija and Trajković, Jelena and Miličić, Dragana and Anđelković, Boban and Lučić, Luka and Savić, Tatjana and Vujisić, Ljubodrag",
year = "2022",
abstract = "In the world of complex smells in natural environment, feeding and mating represent two important olfactory-guided behaviors in Drosophila melanogaster (Diptera: Drosophilidae). Diet affects the chemoprofile composition of the individuals, which, indirectly, may significantly affect their mating success. In this study, chemoprofiles of recently mated flies belonging to four D. melanogaster strains, which were fed for many generations on different substrates (standard cornmeal-S strain; banana-B strain; carrot-C strain; tomato-T strain) were identified and quantified. In total, 67 chemical compounds were identified: 48 compounds were extracted from males maintained on banana and carrot, and 47 compounds from males maintained on cornmeal and tomato substrates, while total of 60 compounds were identified in females from all strains. The strains and the sexes significantly differed in qualitative nature of their chemoprofiles after mating. Significant differences in the relative amount of three major male pheromones (cis-vaccenyl acetate-cVA, (Z)-7-pentacosene, and (Z)-7-tricosene) and in female pheromone (Z,Z)-7,11-nonacosadiene among strains were also recorded. Furthermore, multivariate analysis of variance (MANOVA) pointed to significant differences between virgin and mated individuals of all strains and within both sexes. Differences in some of the well known sex pheromones were also identified when comparing their relative amount before and after mating. The presence of typical male pheromones in females, and vice versa may indicate their bidirectional transfer during copulation. Our results confirm significant effect of mating status on cuticular hydrocarbon (CHC) phenotypes in differently fed D. melanogaster flies.",
publisher = "Hoboken: John Wiley and Sons Inc.",
journal = "Archives of Insect Biochemistry and Physiology, Archives of Insect Biochemistry and Physiology",
title = ""Scent of a fruit fly": Cuticular chemoprofiles after mating in differently fed Drosophila melanogaster (Diptera: Drosophilidae) strains.",
doi = "10.1002/arch.21866",
pages = "e21866"
}

Pavković-Lučić, S., Trajković, J., Miličić, D., Anđelković, B., Lučić, L., Savić, T.,& Vujisić, L.. (2022). "Scent of a fruit fly": Cuticular chemoprofiles after mating in differently fed Drosophila melanogaster (Diptera: Drosophilidae) strains.. in Archives of Insect Biochemistry and Physiology
Hoboken: John Wiley and Sons Inc.., e21866.
https://doi.org/10.1002/arch.21866

Pavković-Lučić S, Trajković J, Miličić D, Anđelković B, Lučić L, Savić T, Vujisić L. "Scent of a fruit fly": Cuticular chemoprofiles after mating in differently fed Drosophila melanogaster (Diptera: Drosophilidae) strains.. in Archives of Insect Biochemistry and Physiology. 2022;:e21866.
doi:10.1002/arch.21866 .

Pavković-Lučić, Sofija, Trajković, Jelena, Miličić, Dragana, Anđelković, Boban, Lučić, Luka, Savić, Tatjana, Vujisić, Ljubodrag, ""Scent of a fruit fly": Cuticular chemoprofiles after mating in differently fed Drosophila melanogaster (Diptera: Drosophilidae) strains." in Archives of Insect Biochemistry and Physiology (2022):e21866,
https://doi.org/10.1002/arch.21866 . .

TY  - CONF
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Tešević, Vele
AU  - Vujisić, Ljubodrag
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4368
AB  - In beetles, secretions are products of different exocrine glands, of which particular 
emphasis is on pygidial glands, which are common for the suborder Adephaga. 
Antimicrobial activity of the pygidial gland secretions of six selected ground beetle 
species was observed against human pathogens. A microdilution method was applied 
in order to determine minimum inhibitory concentrations (MICs), minimum bactericidal 
concentrations (MBCs) and minimum fungicidal concentrations (MFCs). We tested 
16 laboratory and clinical strains of human pathogens (eight bacterial gram-positive 
and gram-negative species, and eight fungal species). The secretions of tested ground 
beetles have a certain level of antimicrobial activity, which differed between species, 
which is caused by the difference in chemical composition of the mixtures. The highest 
antibacterial effect was observed after treatment of most pathogens with secretion of 
Laemostenus punctatus and Carabus ullrichii. The highest resistance of tested pathogenic 
strains was noticed after treatment of the secretion of Calosoma sycophanta, which 
achieved antibacterial effect against E. coli only. The secretions of L. punctatus and C. 
sycophanta each showed a strong antifungal effect compared to positive controls. 
Inhibition of the proliferation of human tumor cell lines and porcine non-tumor 
cells by the secretion of adults of four ground beetle species was observed as well. 
The sulphorhodamine B (SRB) assay was applied to establish GI50 values of the tested 
secretions. All secretions have shown certain antiproliferative effect on the tested 
cell lines, but statistically insignificant in most cases, which confirms the absence of 
cytotoxicity on the non-tumor cell line. Special emphasis is put on the secretion of L. 
punctatus, which reached approximately 50% inhibition of cell proliferation (GI50 value) 
of MCF7 (breast adenocarcinoma) cell line, and showed a potential antitumour activity. 
The antimicrobial, antitumour and antiproliferative potential of the secretions of ground 
beetles was demonstrated for the first time in this study.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade
C3  - The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
T1  - The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4368
ER  - 

@conference{
author = "Nenadić, Marija and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Tešević, Vele and Vujisić, Ljubodrag and Vesović, Nikola and Ćurčić, Srećko",
year = "2019",
abstract = "In beetles, secretions are products of different exocrine glands, of which particular 
emphasis is on pygidial glands, which are common for the suborder Adephaga. 
Antimicrobial activity of the pygidial gland secretions of six selected ground beetle 
species was observed against human pathogens. A microdilution method was applied 
in order to determine minimum inhibitory concentrations (MICs), minimum bactericidal 
concentrations (MBCs) and minimum fungicidal concentrations (MFCs). We tested 
16 laboratory and clinical strains of human pathogens (eight bacterial gram-positive 
and gram-negative species, and eight fungal species). The secretions of tested ground 
beetles have a certain level of antimicrobial activity, which differed between species, 
which is caused by the difference in chemical composition of the mixtures. The highest 
antibacterial effect was observed after treatment of most pathogens with secretion of 
Laemostenus punctatus and Carabus ullrichii. The highest resistance of tested pathogenic 
strains was noticed after treatment of the secretion of Calosoma sycophanta, which 
achieved antibacterial effect against E. coli only. The secretions of L. punctatus and C. 
sycophanta each showed a strong antifungal effect compared to positive controls. 
Inhibition of the proliferation of human tumor cell lines and porcine non-tumor 
cells by the secretion of adults of four ground beetle species was observed as well. 
The sulphorhodamine B (SRB) assay was applied to establish GI50 values of the tested 
secretions. All secretions have shown certain antiproliferative effect on the tested 
cell lines, but statistically insignificant in most cases, which confirms the absence of 
cytotoxicity on the non-tumor cell line. Special emphasis is put on the secretion of L. 
punctatus, which reached approximately 50% inhibition of cell proliferation (GI50 value) 
of MCF7 (breast adenocarcinoma) cell line, and showed a potential antitumour activity. 
The antimicrobial, antitumour and antiproliferative potential of the secretions of ground 
beetles was demonstrated for the first time in this study.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade",
journal = "The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia",
title = "The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4368"
}

Nenadić, M., Soković, M., Glamočlija, J., Ćirić, A., Tešević, V., Vujisić, L., Vesović, N.,& Ćurčić, S.. (2019). The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products. in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade., 43.
https://hdl.handle.net/21.15107/rcub_ibiss_4368

Nenadić M, Soković M, Glamočlija J, Ćirić A, Tešević V, Vujisić L, Vesović N, Ćurčić S. The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products. in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia. 2019;:43.
https://hdl.handle.net/21.15107/rcub_ibiss_4368 .

Nenadić, Marija, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Tešević, Vele, Vujisić, Ljubodrag, Vesović, Nikola, Ćurčić, Srećko, "The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products" in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia (2019):43,
https://hdl.handle.net/21.15107/rcub_ibiss_4368 .

TY  - JOUR
AU  - Bukvički, Danka
AU  - Giweli, Abdulhmid
AU  - Stojković, Dejan
AU  - Vujisić, Ljubodrag
AU  - Tešević, Vele
AU  - Nikolić, Miloš
AU  - Soković, Marina
AU  - Marin, Petar D
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3032
UR  - http://www.journalofdairyscience.org/article/S0022-0302(18)30148-6/fulltext
AB  - The essential oil of Thymus algeriensis was analyzed as a potential preservative in soft cheese. We developed a novel method to test the preserving properties of essential oil in soft cheese. Contamination incidence of Penicillium aurantiogriseum was absent after 30 d of storage at 4°C with 25 µL of essential oil added. The antimicrobial activity was tested against 8 bacteria and 8 fungi. Thymus algeriensis oil showed inhibitory activity against tested bacteria at 0.03 to 0.09 mg/mL, and bactericidal activity was achieved at 0.05 to 0.15 mg/mL. For antifungal activity, minimum inhibitory concentrations ranged between 0.01 and 0.04 mg/mL and minimum fungicidal concentrations between 0.01 and 0.04 mg/mL. Furthermore, the oil was also screened for antiradical activity using the 1,1-diphenyl-2-picrylhydrazyl assay. The results showed that the oil was active and achieved half-maximal inhibitory activity at 0.132 mg/mL. We used gas chromatography, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectrometry to investigate the volatile compounds from the oil. Carvacrol was identified as the main compound in the oil, represented by 80.9% of the total constituents, followed by p-cymene (7.7%).
T2  - Journal of Dairy Science
T1  - Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative.
IS  - 5
VL  - 101
DO  - 10.3168/jds.2017-13714
SP  - 3859
EP  - 3865
ER  - 

@article{
author = "Bukvički, Danka and Giweli, Abdulhmid and Stojković, Dejan and Vujisić, Ljubodrag and Tešević, Vele and Nikolić, Miloš and Soković, Marina and Marin, Petar D",
year = "2018",
abstract = "The essential oil of Thymus algeriensis was analyzed as a potential preservative in soft cheese. We developed a novel method to test the preserving properties of essential oil in soft cheese. Contamination incidence of Penicillium aurantiogriseum was absent after 30 d of storage at 4°C with 25 µL of essential oil added. The antimicrobial activity was tested against 8 bacteria and 8 fungi. Thymus algeriensis oil showed inhibitory activity against tested bacteria at 0.03 to 0.09 mg/mL, and bactericidal activity was achieved at 0.05 to 0.15 mg/mL. For antifungal activity, minimum inhibitory concentrations ranged between 0.01 and 0.04 mg/mL and minimum fungicidal concentrations between 0.01 and 0.04 mg/mL. Furthermore, the oil was also screened for antiradical activity using the 1,1-diphenyl-2-picrylhydrazyl assay. The results showed that the oil was active and achieved half-maximal inhibitory activity at 0.132 mg/mL. We used gas chromatography, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectrometry to investigate the volatile compounds from the oil. Carvacrol was identified as the main compound in the oil, represented by 80.9% of the total constituents, followed by p-cymene (7.7%).",
journal = "Journal of Dairy Science",
title = "Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative.",
number = "5",
volume = "101",
doi = "10.3168/jds.2017-13714",
pages = "3859-3865"
}

Bukvički, D., Giweli, A., Stojković, D., Vujisić, L., Tešević, V., Nikolić, M., Soković, M.,& Marin, P. D.. (2018). Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative.. in Journal of Dairy Science, 101(5), 3859-3865.
https://doi.org/10.3168/jds.2017-13714

Bukvički D, Giweli A, Stojković D, Vujisić L, Tešević V, Nikolić M, Soković M, Marin PD. Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative.. in Journal of Dairy Science. 2018;101(5):3859-3865.
doi:10.3168/jds.2017-13714 .

Bukvički, Danka, Giweli, Abdulhmid, Stojković, Dejan, Vujisić, Ljubodrag, Tešević, Vele, Nikolić, Miloš, Soković, Marina, Marin, Petar D, "Short communication: Cheese supplemented with Thymus algeriensis oil, a potential natural food preservative." in Journal of Dairy Science, 101, no. 5 (2018):3859-3865,
https://doi.org/10.3168/jds.2017-13714 . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Perić Mataruga, Vesna
AU  - Ilijin, Larisa
AU  - Tešević, Vele
AU  - Todosijević, Marina
AU  - Vujisić, Ljubodrag
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2017
UR  - http://link.springer.com/10.1007/s00253-016-8082-7
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-85008708376&origin=SingleRecordEmailAlert&dgcid=scalert_sc_search_email&txGid=BCBFF82A73D51FA0ED62BC41FE5E5987.wsnAw8kcdt7IPYLO0V48gA%3A37
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2511
AB  - Recently, various insect secretions have been tested as possible antimicrobial agents. In beetles, these secretions are essentially products of various exocrine glands, of which particular emphasis is on pygidial glands that are common for the suborder Adephaga. The antimicrobial potential of the pygidial gland secretion isolated from the adults of Calosoma sycophanta against human pathogens has been tested and compared with the potential of other carabid species, particularly within the tribe Carabini. The antimicrobial assay includes a microdilution method which was applied in order to determine the minimal inhibitory, minimal fungicidal and minimal bactericidal concentrations. It has been tested the effect of the secretion against eight strains of fungal and eight strains of bacterial species. The secretion sample of the tested carabid species has shown statistically significant antifungal effect against all strains of treated micromycetes, the highest in comparison with previously tested carabids (Carabus spp., Laemostenus punctatus and Abax parallelepipedus). Aspergillus versicolor proved to be the most sensitive micromycete, while the remaining seven fungal strains have shown the same level of sensibility. In comparison with commercial mycotics ketoconazole and bifonazole, applied as positive controls, the tested secretion showed much higher antifungal activity for all fungal strains. Antibacterial effect has been manifested only against one bacterial strain (Escherichia coli), contrary to other previously studied carabid species. These observations might have a significant impact on the ecological domain and possible purpose in biomedical studies and applications in the future. Additionally, morpho-histology of the pygidial glands of C. sycophanta is investigated and discussed.
T2  - Applied Microbiology and Biotechnology
T1  - The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens
IS  - 3
VL  - 101
DO  - 10.1007/s00253-016-8082-7
SP  - 977
EP  - 985
ER  - 

@article{
author = "Nenadić, Marija and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Perić Mataruga, Vesna and Ilijin, Larisa and Tešević, Vele and Todosijević, Marina and Vujisić, Ljubodrag and Vesović, Nikola and Ćurčić, Srećko",
year = "2017",
abstract = "Recently, various insect secretions have been tested as possible antimicrobial agents. In beetles, these secretions are essentially products of various exocrine glands, of which particular emphasis is on pygidial glands that are common for the suborder Adephaga. The antimicrobial potential of the pygidial gland secretion isolated from the adults of Calosoma sycophanta against human pathogens has been tested and compared with the potential of other carabid species, particularly within the tribe Carabini. The antimicrobial assay includes a microdilution method which was applied in order to determine the minimal inhibitory, minimal fungicidal and minimal bactericidal concentrations. It has been tested the effect of the secretion against eight strains of fungal and eight strains of bacterial species. The secretion sample of the tested carabid species has shown statistically significant antifungal effect against all strains of treated micromycetes, the highest in comparison with previously tested carabids (Carabus spp., Laemostenus punctatus and Abax parallelepipedus). Aspergillus versicolor proved to be the most sensitive micromycete, while the remaining seven fungal strains have shown the same level of sensibility. In comparison with commercial mycotics ketoconazole and bifonazole, applied as positive controls, the tested secretion showed much higher antifungal activity for all fungal strains. Antibacterial effect has been manifested only against one bacterial strain (Escherichia coli), contrary to other previously studied carabid species. These observations might have a significant impact on the ecological domain and possible purpose in biomedical studies and applications in the future. Additionally, morpho-histology of the pygidial glands of C. sycophanta is investigated and discussed.",
journal = "Applied Microbiology and Biotechnology",
title = "The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens",
number = "3",
volume = "101",
doi = "10.1007/s00253-016-8082-7",
pages = "977-985"
}

Nenadić, M., Soković, M., Glamočlija, J., Ćirić, A., Perić Mataruga, V., Ilijin, L., Tešević, V., Todosijević, M., Vujisić, L., Vesović, N.,& Ćurčić, S.. (2017). The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens. in Applied Microbiology and Biotechnology, 101(3), 977-985.
https://doi.org/10.1007/s00253-016-8082-7

Nenadić M, Soković M, Glamočlija J, Ćirić A, Perić Mataruga V, Ilijin L, Tešević V, Todosijević M, Vujisić L, Vesović N, Ćurčić S. The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens. in Applied Microbiology and Biotechnology. 2017;101(3):977-985.
doi:10.1007/s00253-016-8082-7 .

Nenadić, Marija, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Perić Mataruga, Vesna, Ilijin, Larisa, Tešević, Vele, Todosijević, Marina, Vujisić, Ljubodrag, Vesović, Nikola, Ćurčić, Srećko, "The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens" in Applied Microbiology and Biotechnology, 101, no. 3 (2017):977-985,
https://doi.org/10.1007/s00253-016-8082-7 . .

TY  - JOUR
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Perić Mataruga, Vesna
AU  - Krstić, Gordana
AU  - Nenadić, Marija
AU  - Cvetković, Mirjana
AU  - Ilijin, Larisa
AU  - Stanković, Jovana
AU  - Ćurčić, Srećko
PY  - 2017
UR  - https://www.tandfonline.com/doi/full/10.1080/00222933.2017.1293183
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2682
AB  - Although all ground beetles have pygidial glands homologous in structure and function, there are many differences among species, often significant, in terms of chemical composition of the secretion and morphology of the gland components. In this paper, two predatory ground beetle species of the genus Carabus, namely C. (Tomocarabus) convexus and C. (Procrustes) coriaceus, were subjected to chemical, morphological and histological study of their pygidial glands and the glands’ secretions. Altogether, three carboxylic acids were isolated, and the pygidial gland reservoir and other glandular parts are morphologically described in the study. We also tested whether there exist differences in chemical content of the secretion obtained by upsetting the beetles in a traditional way and that obtained directly from intact reservoirs. Detailed data on morphology of the pygidial glands of both species are presented, as well as updated information about the chemical components of the glandular secretions. Apart from tiglic and methacrylic acids, which are typical for Carabus pygidial secretions, we also found benzoic acid as a minor component of the secretion in both species. In addition, a chemotaxonomic overview of Carabus taxa is given in the paper.
T2  - Journal of Natural History
T1  - Chemical secretion and morpho-histology of the pygidial glands in two Palaearctic predatory ground beetle species: Carabus ( Tomocarabus ) convexus and C . ( Procrustes ) coriaceus (Coleoptera: Carabidae)
IS  - 9-10
VL  - 51
DO  - 10.1080/00222933.2017.1293183
SP  - 545
EP  - 560
ER  - 

@article{
author = "Vesović, Nikola and Vujisić, Ljubodrag and Perić Mataruga, Vesna and Krstić, Gordana and Nenadić, Marija and Cvetković, Mirjana and Ilijin, Larisa and Stanković, Jovana and Ćurčić, Srećko",
year = "2017",
abstract = "Although all ground beetles have pygidial glands homologous in structure and function, there are many differences among species, often significant, in terms of chemical composition of the secretion and morphology of the gland components. In this paper, two predatory ground beetle species of the genus Carabus, namely C. (Tomocarabus) convexus and C. (Procrustes) coriaceus, were subjected to chemical, morphological and histological study of their pygidial glands and the glands’ secretions. Altogether, three carboxylic acids were isolated, and the pygidial gland reservoir and other glandular parts are morphologically described in the study. We also tested whether there exist differences in chemical content of the secretion obtained by upsetting the beetles in a traditional way and that obtained directly from intact reservoirs. Detailed data on morphology of the pygidial glands of both species are presented, as well as updated information about the chemical components of the glandular secretions. Apart from tiglic and methacrylic acids, which are typical for Carabus pygidial secretions, we also found benzoic acid as a minor component of the secretion in both species. In addition, a chemotaxonomic overview of Carabus taxa is given in the paper.",
journal = "Journal of Natural History",
title = "Chemical secretion and morpho-histology of the pygidial glands in two Palaearctic predatory ground beetle species: Carabus ( Tomocarabus ) convexus and C . ( Procrustes ) coriaceus (Coleoptera: Carabidae)",
number = "9-10",
volume = "51",
doi = "10.1080/00222933.2017.1293183",
pages = "545-560"
}

Vesović, N., Vujisić, L., Perić Mataruga, V., Krstić, G., Nenadić, M., Cvetković, M., Ilijin, L., Stanković, J.,& Ćurčić, S.. (2017). Chemical secretion and morpho-histology of the pygidial glands in two Palaearctic predatory ground beetle species: Carabus ( Tomocarabus ) convexus and C . ( Procrustes ) coriaceus (Coleoptera: Carabidae). in Journal of Natural History, 51(9-10), 545-560.
https://doi.org/10.1080/00222933.2017.1293183

Vesović N, Vujisić L, Perić Mataruga V, Krstić G, Nenadić M, Cvetković M, Ilijin L, Stanković J, Ćurčić S. Chemical secretion and morpho-histology of the pygidial glands in two Palaearctic predatory ground beetle species: Carabus ( Tomocarabus ) convexus and C . ( Procrustes ) coriaceus (Coleoptera: Carabidae). in Journal of Natural History. 2017;51(9-10):545-560.
doi:10.1080/00222933.2017.1293183 .

Vesović, Nikola, Vujisić, Ljubodrag, Perić Mataruga, Vesna, Krstić, Gordana, Nenadić, Marija, Cvetković, Mirjana, Ilijin, Larisa, Stanković, Jovana, Ćurčić, Srećko, "Chemical secretion and morpho-histology of the pygidial glands in two Palaearctic predatory ground beetle species: Carabus ( Tomocarabus ) convexus and C . ( Procrustes ) coriaceus (Coleoptera: Carabidae)" in Journal of Natural History, 51, no. 9-10 (2017):545-560,
https://doi.org/10.1080/00222933.2017.1293183 . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Perić Mataruga, Vesna
AU  - Ilijin, Larisa
AU  - Tešević, Vele
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2016
UR  - https://link.springer.com/article/10.1007/s00114-016-1358-z
UR  - https://pubmed.ncbi.nlm.nih.gov/27000261/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3981
AB  - The antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, Carabus ullrichii, C. coriaceus, and Abax parallelepipedus, have been tested. Microdilution method was applied for detection of minimal inhibitory concentrations (MICs), minimal bactericidal concentrations (MBCs), and minimal fungicidal concentrations (MFCs). Additionally, morpho-histology of the pygidial glands is investigated. We have tested 16 laboratory and clinical strains of human pathogens-eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples of C. ullrichii have showed the strongest antimicrobial effect against all strains of treated bacteria and fungi. Staphylococcus aureus, Lysteria monocytogenes, and Salmonella typhimurium proved to be the most sensitive bacterial strains. Penicillium funiculosum proved to be the most sensitive micromycete, while P. ochrochloron and P. verrucosum var. cyclopium the most resistant micromycetes. The pygidial secretion of C. coriaceus has showed antibacterial potential solely against Pseudomonas aeruginosa and antifungal activity against Aspergillus fumigatus, A. versicolor, A. ochraceus, and P. ochrochloron. Antibacterial properties of pygidial gland secretion of A. parallelepipedus were achieved against P. aeruginosa, while antifungal activity was detected against five of the eight tested micromycetes (A. fumigatus, A. versicolor, A. ochraceus, Trichoderma viride, and P. verrucosum var. cyclopium). Commercial antibiotics Streptomycin and Ampicillin and mycotics Ketoconazole and Bifonazole, applied as the positive controls, showed higher antibacterial/antifungal properties for all bacterial and fungal strains. The results of this observation might have a significant impact on the environmental aspects and possible medical purpose in the future.
PB  - Springer Nature
T2  - The Science of Nature
T1  - Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae)
IS  - 3-4
VL  - 103
DO  - 10.1007/s00114-016-1358-z
SP  - 34
ER  - 

@article{
author = "Nenadić, Marija and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Perić Mataruga, Vesna and Ilijin, Larisa and Tešević, Vele and Vujisić, Ljubodrag and Todosijević, Marina and Vesović, Nikola and Ćurčić, Srećko",
year = "2016",
abstract = "The antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, Carabus ullrichii, C. coriaceus, and Abax parallelepipedus, have been tested. Microdilution method was applied for detection of minimal inhibitory concentrations (MICs), minimal bactericidal concentrations (MBCs), and minimal fungicidal concentrations (MFCs). Additionally, morpho-histology of the pygidial glands is investigated. We have tested 16 laboratory and clinical strains of human pathogens-eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples of C. ullrichii have showed the strongest antimicrobial effect against all strains of treated bacteria and fungi. Staphylococcus aureus, Lysteria monocytogenes, and Salmonella typhimurium proved to be the most sensitive bacterial strains. Penicillium funiculosum proved to be the most sensitive micromycete, while P. ochrochloron and P. verrucosum var. cyclopium the most resistant micromycetes. The pygidial secretion of C. coriaceus has showed antibacterial potential solely against Pseudomonas aeruginosa and antifungal activity against Aspergillus fumigatus, A. versicolor, A. ochraceus, and P. ochrochloron. Antibacterial properties of pygidial gland secretion of A. parallelepipedus were achieved against P. aeruginosa, while antifungal activity was detected against five of the eight tested micromycetes (A. fumigatus, A. versicolor, A. ochraceus, Trichoderma viride, and P. verrucosum var. cyclopium). Commercial antibiotics Streptomycin and Ampicillin and mycotics Ketoconazole and Bifonazole, applied as the positive controls, showed higher antibacterial/antifungal properties for all bacterial and fungal strains. The results of this observation might have a significant impact on the environmental aspects and possible medical purpose in the future.",
publisher = "Springer Nature",
journal = "The Science of Nature",
title = "Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae)",
number = "3-4",
volume = "103",
doi = "10.1007/s00114-016-1358-z",
pages = "34"
}

Nenadić, M., Soković, M., Glamočlija, J., Ćirić, A., Perić Mataruga, V., Ilijin, L., Tešević, V., Vujisić, L., Todosijević, M., Vesović, N.,& Ćurčić, S.. (2016). Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae). in The Science of Nature
Springer Nature., 103(3-4), 34.
https://doi.org/10.1007/s00114-016-1358-z

Nenadić M, Soković M, Glamočlija J, Ćirić A, Perić Mataruga V, Ilijin L, Tešević V, Vujisić L, Todosijević M, Vesović N, Ćurčić S. Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae). in The Science of Nature. 2016;103(3-4):34.
doi:10.1007/s00114-016-1358-z .

Nenadić, Marija, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Perić Mataruga, Vesna, Ilijin, Larisa, Tešević, Vele, Vujisić, Ljubodrag, Todosijević, Marina, Vesović, Nikola, Ćurčić, Srećko, "Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae)" in The Science of Nature, 103, no. 3-4 (2016):34,
https://doi.org/10.1007/s00114-016-1358-z . .

TY  - JOUR
AU  - Bukvički, Danka R.
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Vannini, Lucia
AU  - Nissen, Lorenzo
AU  - Novaković, Miroslav
AU  - Vujisić, Ljubodrag
AU  - Asakawa, Yoshinori
AU  - Marin, Petar D.
PY  - 2016
UR  - http://www.naturalproduct.us/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2754
AB  - The chemical composition, antimicrobial and antiquorum sensing activity of the essential oil of Micromeria thymifolia (Scop.) Fritsch were investigated. Limonene, piperitone epoxide and piperitenone epoxide were found as the main constituents using a gas chromatography-mass spectrometry technique. In vitro antimicrobial activity of the oil was tested against six bacterial and seven fungal strains and high antimicrobial potential was noticed. Minimum inhibitory concentration varied from 0.031 mg/mL to 0.5 mg/mL for bacterial and 0.062 mg/mL to 0.5 mg/mL for fungal strains. The antiquorum properties of the essential oil were evaluated on Pseudomonas aeruginosa PAO1. The oil was tested at subMIC concentrations for anti-quorum sensing activity. The analyses on quorum-sensing functions have been carried out by evaluating twitching and swarming of bacterial cultures and the total amount of pyocyanin production produced by P. aeruginosa. This study showed that M. thymifolia essential oil exhibited antiquorum sensing activity and may be used as an antipathogenic drug.
T2  - Natural Product Communications
T1  - Micromeria thymifolia essential oil suppresses quorum-sensing signaling in pseudomonas aeruginosa
IS  - 12
VL  - 11
DO  - 10.1177/1934578x1601101232
SP  - 1903
EP  - 1906
ER  - 

@article{
author = "Bukvički, Danka R. and Ćirić, Ana and Soković, Marina and Vannini, Lucia and Nissen, Lorenzo and Novaković, Miroslav and Vujisić, Ljubodrag and Asakawa, Yoshinori and Marin, Petar D.",
year = "2016",
abstract = "The chemical composition, antimicrobial and antiquorum sensing activity of the essential oil of Micromeria thymifolia (Scop.) Fritsch were investigated. Limonene, piperitone epoxide and piperitenone epoxide were found as the main constituents using a gas chromatography-mass spectrometry technique. In vitro antimicrobial activity of the oil was tested against six bacterial and seven fungal strains and high antimicrobial potential was noticed. Minimum inhibitory concentration varied from 0.031 mg/mL to 0.5 mg/mL for bacterial and 0.062 mg/mL to 0.5 mg/mL for fungal strains. The antiquorum properties of the essential oil were evaluated on Pseudomonas aeruginosa PAO1. The oil was tested at subMIC concentrations for anti-quorum sensing activity. The analyses on quorum-sensing functions have been carried out by evaluating twitching and swarming of bacterial cultures and the total amount of pyocyanin production produced by P. aeruginosa. This study showed that M. thymifolia essential oil exhibited antiquorum sensing activity and may be used as an antipathogenic drug.",
journal = "Natural Product Communications",
title = "Micromeria thymifolia essential oil suppresses quorum-sensing signaling in pseudomonas aeruginosa",
number = "12",
volume = "11",
doi = "10.1177/1934578x1601101232",
pages = "1903-1906"
}

Bukvički, D. R., Ćirić, A., Soković, M., Vannini, L., Nissen, L., Novaković, M., Vujisić, L., Asakawa, Y.,& Marin, P. D.. (2016). Micromeria thymifolia essential oil suppresses quorum-sensing signaling in pseudomonas aeruginosa. in Natural Product Communications, 11(12), 1903-1906.
https://doi.org/10.1177/1934578x1601101232

Bukvički DR, Ćirić A, Soković M, Vannini L, Nissen L, Novaković M, Vujisić L, Asakawa Y, Marin PD. Micromeria thymifolia essential oil suppresses quorum-sensing signaling in pseudomonas aeruginosa. in Natural Product Communications. 2016;11(12):1903-1906.
doi:10.1177/1934578x1601101232 .

Bukvički, Danka R., Ćirić, Ana, Soković, Marina, Vannini, Lucia, Nissen, Lorenzo, Novaković, Miroslav, Vujisić, Ljubodrag, Asakawa, Yoshinori, Marin, Petar D., "Micromeria thymifolia essential oil suppresses quorum-sensing signaling in pseudomonas aeruginosa" in Natural Product Communications, 11, no. 12 (2016):1903-1906,
https://doi.org/10.1177/1934578x1601101232 . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Perić Mataruga, Vesna
AU  - Tešević, Vele
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2016
UR  - https://www.cambridge.org/core/journals/bulletin-of-entomological-research/article/antimicrobial-activity-of-the-pygidial-gland-secretion-of-the-troglophilic-ground-beetle-laemostenus-pristonychus-punctatus-dejean-1828-insecta-coleoptera-carabidae/8DF8DE1725619065F0C0C1631B5CF359
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3970
AB  - The antimicrobial activity of the pygidial gland secretion released by adult individuals
of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean,
1828), applying microdilution method with the aim to detect minimal inhibitory concentration,
minimal bactericidal concentration and minimal fungicidal concentration,
has been investigated. In addition, morphology of the pygidial glands is observed.
Wehave tested 16 laboratory and clinical strains of human pathogens – eight bacterial
both gram-positive and gram-negative species and eight fungal species. The pygidial
secretion samples have showed antimicrobial properties against all strains of treated
bacteria and fungi. Micrococcus flavus proved to be more resistant compared with
other bacterial strains. More significant antimicrobial properties of the secretion are
observed against Escherichia coli, which proved to be the most sensitive bacteria.
Aspergillus fumigatus proved to be the most resistant, while Penicillium ochrochloron and
Penicillium verrucosum var. cyclopium themost sensitive micromycetes. Commercial antibiotics
Streptomycin and Ampicillin and antimycotics Ketoconazole and Bifonazole, applied
as positive controls, showed higher antibacterial properties for all bacterial and
fungal strains, except for P. ochrochloron, which proved to be more resistant on
Ketoconazole compared with the pygidial gland secretion of L. (P.) punctatus. Apart
from the role in ecological aspects, the antimicrobial properties of the tested secretion
possibly might have medical significance in the future.
PB  - Cambridge University Press
T2  - Bulletin of Entomological Research
T1  - Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae)
IS  - 4
VL  - 106
DO  - 10.1017/S0007485316000109
SP  - 474
EP  - 480
ER  - 

@article{
author = "Nenadić, Marija and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Perić Mataruga, Vesna and Tešević, Vele and Vujisić, Ljubodrag and Todosijević, Marina and Vesović, Nikola and Ćurčić, Srećko",
year = "2016",
abstract = "The antimicrobial activity of the pygidial gland secretion released by adult individuals
of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean,
1828), applying microdilution method with the aim to detect minimal inhibitory concentration,
minimal bactericidal concentration and minimal fungicidal concentration,
has been investigated. In addition, morphology of the pygidial glands is observed.
Wehave tested 16 laboratory and clinical strains of human pathogens – eight bacterial
both gram-positive and gram-negative species and eight fungal species. The pygidial
secretion samples have showed antimicrobial properties against all strains of treated
bacteria and fungi. Micrococcus flavus proved to be more resistant compared with
other bacterial strains. More significant antimicrobial properties of the secretion are
observed against Escherichia coli, which proved to be the most sensitive bacteria.
Aspergillus fumigatus proved to be the most resistant, while Penicillium ochrochloron and
Penicillium verrucosum var. cyclopium themost sensitive micromycetes. Commercial antibiotics
Streptomycin and Ampicillin and antimycotics Ketoconazole and Bifonazole, applied
as positive controls, showed higher antibacterial properties for all bacterial and
fungal strains, except for P. ochrochloron, which proved to be more resistant on
Ketoconazole compared with the pygidial gland secretion of L. (P.) punctatus. Apart
from the role in ecological aspects, the antimicrobial properties of the tested secretion
possibly might have medical significance in the future.",
publisher = "Cambridge University Press",
journal = "Bulletin of Entomological Research",
title = "Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae)",
number = "4",
volume = "106",
doi = "10.1017/S0007485316000109",
pages = "474-480"
}

Nenadić, M., Soković, M., Glamočlija, J., Ćirić, A., Perić Mataruga, V., Tešević, V., Vujisić, L., Todosijević, M., Vesović, N.,& Ćurčić, S.. (2016). Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae). in Bulletin of Entomological Research
Cambridge University Press., 106(4), 474-480.
https://doi.org/10.1017/S0007485316000109

Nenadić M, Soković M, Glamočlija J, Ćirić A, Perić Mataruga V, Tešević V, Vujisić L, Todosijević M, Vesović N, Ćurčić S. Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae). in Bulletin of Entomological Research. 2016;106(4):474-480.
doi:10.1017/S0007485316000109 .

Nenadić, Marija, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Perić Mataruga, Vesna, Tešević, Vele, Vujisić, Ljubodrag, Todosijević, Marina, Vesović, Nikola, Ćurčić, Srećko, "Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae)" in Bulletin of Entomological Research, 106, no. 4 (2016):474-480,
https://doi.org/10.1017/S0007485316000109 . .

TY  - JOUR
AU  - Gođevac, Dejan
AU  - Soković, Marina
AU  - Vujisić, Ljubodrag
AU  - Vučković, Ivan
AU  - Vajs, Vlatka
AU  - Marin, Petar D.
AU  - Tešević, Vele
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/564
AB  - An investigation of the chemical composition and antimicrobial activity of the essential oil of Galatella linosyris is presented. The chemical analysis (GC/MS, NMR) showed that sabinene (40 %), β-pinene (35.5 %), α-pinene (4.5 %), limonene (4 %), γ-muurolene (4 %), and (E)-caryophyllene (3.3 %) were dominant components in this oil. Microdilution assays were used to evaluate the minimum inhibitory concentration (MIC) and the minimum bactericidal/ fungicidal concentrations (MBC/MFC). G. linosyris essential oil exhibited better antibacterial activity against some of the tested bacteria than antifungal activity.
AB  - U ovom radu je prikazano ispitivanje hemijskog sastava i antimikrobne aktivnosti etarskog ulja biljne vrste Galatella linosyris. Hemijskom analizom (GC-MS i NMR) je utvrđeno da su glavni sastojci ovog etarskog ulja sabinen (40 %), β-pinen (35,5 %) , α-pinen (4,5 %), limonen (4 %), γ-muurolen (4 %) i (E)-kariofilen (3,3 %). Za procenu minimalne inhibitorne koncentracije (MIC) i minimalne baktericidne/fungicidne koncentracije (MBC/ /MFC) korišćeni su testovi mikrorazblaženja. Etarsko ulje G. linosyris je pokazalo bolju antibakterijsku nego antifungalnu aktivnost.
T2  - Journal of the Serbian Chemical Society
T1  - Sastav i antimikrobna aktivnost etarskog ulja iz Galatella linosyris (L.) Rchb. f. (Asteraceae)
T1  - Composition and antimicrobial activity of the essential oil from Galatella linosyris (L.) Rchb. f. (Asteraceae)
IS  - 5
VL  - 77
SP  - 619
EP  - 626
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_564
ER  - 

@article{
author = "Gođevac, Dejan and Soković, Marina and Vujisić, Ljubodrag and Vučković, Ivan and Vajs, Vlatka and Marin, Petar D. and Tešević, Vele",
year = "2012, 2012",
abstract = "An investigation of the chemical composition and antimicrobial activity of the essential oil of Galatella linosyris is presented. The chemical analysis (GC/MS, NMR) showed that sabinene (40 %), β-pinene (35.5 %), α-pinene (4.5 %), limonene (4 %), γ-muurolene (4 %), and (E)-caryophyllene (3.3 %) were dominant components in this oil. Microdilution assays were used to evaluate the minimum inhibitory concentration (MIC) and the minimum bactericidal/ fungicidal concentrations (MBC/MFC). G. linosyris essential oil exhibited better antibacterial activity against some of the tested bacteria than antifungal activity., U ovom radu je prikazano ispitivanje hemijskog sastava i antimikrobne aktivnosti etarskog ulja biljne vrste Galatella linosyris. Hemijskom analizom (GC-MS i NMR) je utvrđeno da su glavni sastojci ovog etarskog ulja sabinen (40 %), β-pinen (35,5 %) , α-pinen (4,5 %), limonen (4 %), γ-muurolen (4 %) i (E)-kariofilen (3,3 %). Za procenu minimalne inhibitorne koncentracije (MIC) i minimalne baktericidne/fungicidne koncentracije (MBC/ /MFC) korišćeni su testovi mikrorazblaženja. Etarsko ulje G. linosyris je pokazalo bolju antibakterijsku nego antifungalnu aktivnost.",
journal = "Journal of the Serbian Chemical Society",
title = "Sastav i antimikrobna aktivnost etarskog ulja iz Galatella linosyris (L.) Rchb. f. (Asteraceae), Composition and antimicrobial activity of the essential oil from Galatella linosyris (L.) Rchb. f. (Asteraceae)",
number = "5",
volume = "77",
pages = "619-626",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_564"
}

Gođevac, D., Soković, M., Vujisić, L., Vučković, I., Vajs, V., Marin, P. D.,& Tešević, V.. (2012). Sastav i antimikrobna aktivnost etarskog ulja iz Galatella linosyris (L.) Rchb. f. (Asteraceae). in Journal of the Serbian Chemical Society, 77(5), 619-626.
https://hdl.handle.net/21.15107/rcub_ibiss_564

Gođevac D, Soković M, Vujisić L, Vučković I, Vajs V, Marin PD, Tešević V. Sastav i antimikrobna aktivnost etarskog ulja iz Galatella linosyris (L.) Rchb. f. (Asteraceae). in Journal of the Serbian Chemical Society. 2012;77(5):619-626.
https://hdl.handle.net/21.15107/rcub_ibiss_564 .

Gođevac, Dejan, Soković, Marina, Vujisić, Ljubodrag, Vučković, Ivan, Vajs, Vlatka, Marin, Petar D., Tešević, Vele, "Sastav i antimikrobna aktivnost etarskog ulja iz Galatella linosyris (L.) Rchb. f. (Asteraceae)" in Journal of the Serbian Chemical Society, 77, no. 5 (2012):619-626,
https://hdl.handle.net/21.15107/rcub_ibiss_564 .