TY  - JOUR
AU  - Mileski, Ksenija S.
AU  - Ćirić, Ana
AU  - Gašić, Uroš
AU  - Žarković, Lazar D.
AU  - Krivošej, Zoran Đ.
AU  - Džamić, Ana M.
PY  - 2023
UR  - https://www.acgpubs.org/doc/20221125121922364-RNP-2207-2528.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5345
AB  - The traditional European medicinal plant Laserpitium siler L. was studied for the first time for the chemical composition and in vitro biological potential of extracts from fruits and aerial parts. Eight extracts were subjected to spectrophotometric detection and LC-MS quantification of phenolics. The bioactivity assessment comprised antioxidant , antimicrobial , anti-denaturation , acetylcholinesterase , tyrosinase , alpha-amylase , and alpha-glucosidase inhibition assays. The spectrophotometric analyses showed that the alcoholic extracts contained the most phenolic acids and coumarins. Among the twenty quantified compounds in the methanolic extracts , chlorogenic acid (29.10 mg/kg in the fruits; 47.00 mg/kg in the aerial parts) and p-coumaric acid (7.50 mg/kg in the fruits; 9.50 mg/kg in the aerial parts) were the most abundant. The DPPH/ABTS/BBT tests showed the strongest antioxidant effect of the methanolic extract of the aerial parts. The results of the microdilution assay referred to stronger antibacterial than antifungal activity , with the essential oil being as effective as the antibiotics used. The extracts expressed promising activity against P. aeruginosa PAO1 biofilm formation and pyocyanin synthesis. The prevention of BSA denaturation by the ethanolic extracts was comparable to diclofenac (91.08%-fruits , 95.08%-aerial parts , and 95.60%-diclofenac). IC50 values showed greater enzyme inhibitory potential of aerial parts and the highest reduction of alpha-glucosidase activity.
T2  - Records of Natural Products
T1  - Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia
IS  - 3
VL  - 17
DO  - 10.25135/rnp.364.2207.2528
SP  - 453
EP  - 475
ER  - 

@article{
author = "Mileski, Ksenija S. and Ćirić, Ana and Gašić, Uroš and Žarković, Lazar D. and Krivošej, Zoran Đ. and Džamić, Ana M.",
year = "2023",
abstract = "The traditional European medicinal plant Laserpitium siler L. was studied for the first time for the chemical composition and in vitro biological potential of extracts from fruits and aerial parts. Eight extracts were subjected to spectrophotometric detection and LC-MS quantification of phenolics. The bioactivity assessment comprised antioxidant , antimicrobial , anti-denaturation , acetylcholinesterase , tyrosinase , alpha-amylase , and alpha-glucosidase inhibition assays. The spectrophotometric analyses showed that the alcoholic extracts contained the most phenolic acids and coumarins. Among the twenty quantified compounds in the methanolic extracts , chlorogenic acid (29.10 mg/kg in the fruits; 47.00 mg/kg in the aerial parts) and p-coumaric acid (7.50 mg/kg in the fruits; 9.50 mg/kg in the aerial parts) were the most abundant. The DPPH/ABTS/BBT tests showed the strongest antioxidant effect of the methanolic extract of the aerial parts. The results of the microdilution assay referred to stronger antibacterial than antifungal activity , with the essential oil being as effective as the antibiotics used. The extracts expressed promising activity against P. aeruginosa PAO1 biofilm formation and pyocyanin synthesis. The prevention of BSA denaturation by the ethanolic extracts was comparable to diclofenac (91.08%-fruits , 95.08%-aerial parts , and 95.60%-diclofenac). IC50 values showed greater enzyme inhibitory potential of aerial parts and the highest reduction of alpha-glucosidase activity.",
journal = "Records of Natural Products",
title = "Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia",
number = "3",
volume = "17",
doi = "10.25135/rnp.364.2207.2528",
pages = "453-475"
}

Mileski, K. S., Ćirić, A., Gašić, U., Žarković, L. D., Krivošej, Z. Đ.,& Džamić, A. M.. (2023). Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia. in Records of Natural Products, 17(3), 453-475.
https://doi.org/10.25135/rnp.364.2207.2528

Mileski KS, Ćirić A, Gašić U, Žarković LD, Krivošej ZĐ, Džamić AM. Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia. in Records of Natural Products. 2023;17(3):453-475.
doi:10.25135/rnp.364.2207.2528 .

Mileski, Ksenija S., Ćirić, Ana, Gašić, Uroš, Žarković, Lazar D., Krivošej, Zoran Đ., Džamić, Ana M., "Comparative Analyses on Chemical Constituents and Biological Activities of Laserpitium siler L. from Serbia" in Records of Natural Products, 17, no. 3 (2023):453-475,
https://doi.org/10.25135/rnp.364.2207.2528 . .

TY  - JOUR
AU  - Žarković, Lazar D.
AU  - Mileski, Ksenija S.
AU  - Matejić, Jelena S.
AU  - Gašić, Uroš
AU  - Rajčević, Nemanja F.
AU  - Marin, Petar
AU  - Džamić, Ana M.
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2212429222005855
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5347
AB  - In this study, the chemical composition, antioxidant, and hypoglycemic activity of Rosa arvensis Huds. leaves and fruits collected from Zlatibor, Željin, Veliki Krš mountains and Petnica village (Serbia) were analysed. Phenolic and vitamin profiles, as well as in vitro antioxidant potential and enzyme inhibitory activity on α-amylase and α-glucosidase, were studied. Phytochemical analysis showed higher phenolic content in leaf extracts than in those obtained from fruits. Gallic, chlorogenic and syringic acids were the most abundant phenolic acids, while catechin was the major flavonoid compound. Furthermore, the highest level of α-tocopherol (1.94 mg/100 g fw) in achenes and ascorbic acid (9.60 mg/100 g fw) in hypanthium were quantified in the fruits sampled on the Veliki Krš Mt. High antioxidant capacity was observed for the leaf extracts. It was highly correlated with their chemical composition and primarily referred to the catechin content (r > 0.95, p < 0.01), while the quinic acid had the opposite effect (r > −0.97, p < 0.01). Leaf extracts also showed good α-glucosidase and moderate α-amylase inhibitory potential in contrast to hypanthium and achenes extracts with lower antidiabetic activity. The high phenolic content and noted antioxidative potential were strongly correlated with the α-glucosidase inhibitory activity (r > 0.80, p < 0.01), while the α-amylase inhibition was only moderately correlated with those compounds (r > 0.49, p < 0.01). Among the tested R. arvensis samples, those from Zlatibor Mt. contained the highest amount of target compounds and expressed the highest biological activity. The obtained results suggest that R. arvensis extracts present a new source of bioactive compounds that could be implemented as novel food ingredients.
PB  - Elsevier Ltd.
T2  - Food Bioscience
T1  - Phytochemical characterisation, in vitro antioxidant and antidiabetic activity of Rosa arvensis Huds. extracts
VL  - 50
DO  - 10.1016/j.fbio.2022.102125
SP  - 102125
ER  - 

@article{
author = "Žarković, Lazar D. and Mileski, Ksenija S. and Matejić, Jelena S. and Gašić, Uroš and Rajčević, Nemanja F. and Marin, Petar and Džamić, Ana M.",
year = "2022",
abstract = "In this study, the chemical composition, antioxidant, and hypoglycemic activity of Rosa arvensis Huds. leaves and fruits collected from Zlatibor, Željin, Veliki Krš mountains and Petnica village (Serbia) were analysed. Phenolic and vitamin profiles, as well as in vitro antioxidant potential and enzyme inhibitory activity on α-amylase and α-glucosidase, were studied. Phytochemical analysis showed higher phenolic content in leaf extracts than in those obtained from fruits. Gallic, chlorogenic and syringic acids were the most abundant phenolic acids, while catechin was the major flavonoid compound. Furthermore, the highest level of α-tocopherol (1.94 mg/100 g fw) in achenes and ascorbic acid (9.60 mg/100 g fw) in hypanthium were quantified in the fruits sampled on the Veliki Krš Mt. High antioxidant capacity was observed for the leaf extracts. It was highly correlated with their chemical composition and primarily referred to the catechin content (r > 0.95, p < 0.01), while the quinic acid had the opposite effect (r > −0.97, p < 0.01). Leaf extracts also showed good α-glucosidase and moderate α-amylase inhibitory potential in contrast to hypanthium and achenes extracts with lower antidiabetic activity. The high phenolic content and noted antioxidative potential were strongly correlated with the α-glucosidase inhibitory activity (r > 0.80, p < 0.01), while the α-amylase inhibition was only moderately correlated with those compounds (r > 0.49, p < 0.01). Among the tested R. arvensis samples, those from Zlatibor Mt. contained the highest amount of target compounds and expressed the highest biological activity. The obtained results suggest that R. arvensis extracts present a new source of bioactive compounds that could be implemented as novel food ingredients.",
publisher = "Elsevier Ltd.",
journal = "Food Bioscience",
title = "Phytochemical characterisation, in vitro antioxidant and antidiabetic activity of Rosa arvensis Huds. extracts",
volume = "50",
doi = "10.1016/j.fbio.2022.102125",
pages = "102125"
}

Žarković, L. D., Mileski, K. S., Matejić, J. S., Gašić, U., Rajčević, N. F., Marin, P.,& Džamić, A. M.. (2022). Phytochemical characterisation, in vitro antioxidant and antidiabetic activity of Rosa arvensis Huds. extracts. in Food Bioscience
Elsevier Ltd.., 50, 102125.
https://doi.org/10.1016/j.fbio.2022.102125

Žarković LD, Mileski KS, Matejić JS, Gašić U, Rajčević NF, Marin P, Džamić AM. Phytochemical characterisation, in vitro antioxidant and antidiabetic activity of Rosa arvensis Huds. extracts. in Food Bioscience. 2022;50:102125.
doi:10.1016/j.fbio.2022.102125 .

Žarković, Lazar D., Mileski, Ksenija S., Matejić, Jelena S., Gašić, Uroš, Rajčević, Nemanja F., Marin, Petar, Džamić, Ana M., "Phytochemical characterisation, in vitro antioxidant and antidiabetic activity of Rosa arvensis Huds. extracts" in Food Bioscience, 50 (2022):102125,
https://doi.org/10.1016/j.fbio.2022.102125 . .

TY  - JOUR
AU  - Džamić, Ana M.
AU  - Mileski, Ksenija S.
AU  - Ćirić, Ana
AU  - Ristić, Mihailo S.
AU  - Soković, Marina
AU  - Marin, Petar D.
PY  - 2019
UR  - https://www.tandfonline.com/doi/full/10.1080/0972060X.2019.1611487
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3384
AB  - The main aim of this work was to determine the chemical composition of essential oil from aerial parts and to evaluate potential antimicrobial and antioxidant activities of essential oil and deodorized extracts of Helichrysum italicum. The gas chromatography-flame ionization detector (GC/FID) and gas chromatography-mass spectrometry (GC/MS) techniques revealed the highest amount of sesquiterpene hydrocarbons (37.88 %), with neryl-acetate (20.44 %), γ-curcumene (14.08 %) and trans-α-bergamotene (6.99 %) as the main components. Total phenolic contents of tested extracts were determined by Folin-Ciocalteau assay and gallic acid was used as a standard. The highest phenolic concentration was measured in deodorised aqueous extract. The radical scavenging activity of the samples was evaluated by means of DPPH radicals. In this test, deodorised aqueous extract possessed the highest antioxidant effect (IC50 = 0.35 mg/ml), followed by deodorized methanol extract, while essential oil had the lowest scavengin...
T2  - Journal of Essential Oil Bearing Plants
T2  - Journal of Essential Oil Bearing Plants
T1  - Essential Oil Composition, Antioxidant and Antimicrobial Properties of Essential Oil and Deodorized Extracts of Helichrysum italicum (Roth) G. Don
IS  - 2
VL  - 22
DO  - 10.1080/0972060X.2019.1611487
SP  - 493
EP  - 503
ER  - 

@article{
author = "Džamić, Ana M. and Mileski, Ksenija S. and Ćirić, Ana and Ristić, Mihailo S. and Soković, Marina and Marin, Petar D.",
year = "2019",
abstract = "The main aim of this work was to determine the chemical composition of essential oil from aerial parts and to evaluate potential antimicrobial and antioxidant activities of essential oil and deodorized extracts of Helichrysum italicum. The gas chromatography-flame ionization detector (GC/FID) and gas chromatography-mass spectrometry (GC/MS) techniques revealed the highest amount of sesquiterpene hydrocarbons (37.88 %), with neryl-acetate (20.44 %), γ-curcumene (14.08 %) and trans-α-bergamotene (6.99 %) as the main components. Total phenolic contents of tested extracts were determined by Folin-Ciocalteau assay and gallic acid was used as a standard. The highest phenolic concentration was measured in deodorised aqueous extract. The radical scavenging activity of the samples was evaluated by means of DPPH radicals. In this test, deodorised aqueous extract possessed the highest antioxidant effect (IC50 = 0.35 mg/ml), followed by deodorized methanol extract, while essential oil had the lowest scavengin...",
journal = "Journal of Essential Oil Bearing Plants, Journal of Essential Oil Bearing Plants",
title = "Essential Oil Composition, Antioxidant and Antimicrobial Properties of Essential Oil and Deodorized Extracts of Helichrysum italicum (Roth) G. Don",
number = "2",
volume = "22",
doi = "10.1080/0972060X.2019.1611487",
pages = "493-503"
}

Džamić, A. M., Mileski, K. S., Ćirić, A., Ristić, M. S., Soković, M.,& Marin, P. D.. (2019). Essential Oil Composition, Antioxidant and Antimicrobial Properties of Essential Oil and Deodorized Extracts of Helichrysum italicum (Roth) G. Don. in Journal of Essential Oil Bearing Plants, 22(2), 493-503.
https://doi.org/10.1080/0972060X.2019.1611487

Džamić AM, Mileski KS, Ćirić A, Ristić MS, Soković M, Marin PD. Essential Oil Composition, Antioxidant and Antimicrobial Properties of Essential Oil and Deodorized Extracts of Helichrysum italicum (Roth) G. Don. in Journal of Essential Oil Bearing Plants. 2019;22(2):493-503.
doi:10.1080/0972060X.2019.1611487 .

Džamić, Ana M., Mileski, Ksenija S., Ćirić, Ana, Ristić, Mihailo S., Soković, Marina, Marin, Petar D., "Essential Oil Composition, Antioxidant and Antimicrobial Properties of Essential Oil and Deodorized Extracts of Helichrysum italicum (Roth) G. Don" in Journal of Essential Oil Bearing Plants, 22, no. 2 (2019):493-503,
https://doi.org/10.1080/0972060X.2019.1611487 . .

TY  - JOUR
AU  - Mileski, Ksenija S.
AU  - Trifunović, Snežana S.
AU  - Ćirić, Ana
AU  - Šakić, Željana M.
AU  - Ristić, Mihailo S.
AU  - Todorović, Nina M.
AU  - Matevski, Vlado S.
AU  - Marin, Petar D.
AU  - Tešević, Vele V.
AU  - Džamić, Ana M.
PY  - 2017
UR  - http://pubs.acs.org/doi/10.1021/acs.jafc.7b04202
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2952
AB  - The essential oil, different extracts, and isolated compounds of Angelica pancicii Vandas (Apiaceae) were investigated for the first time. The GC-FID and GC-MS analyses revealed sesquiterpenoids as the main constituents of A. pancicii essential oil of aerial parts with bornyl acetate (8.08%), n-octanol (5.82%), kessane (4.26%), and β-selinene (4.26%) as the main constituents. Analysis of methanol extracts, using an HPLC-DAD/ESI-ToF-MS system, showed a total of 52 compounds in the aerial parts and 53 in the roots, indicating coumarins as the main constituents. In addition, new chromone (1) and six known furanocoumarins (2-7) were isolated from the roots and structurally elucidated by combined spectroscopic methods. The aerial part extracts exhibited higher polyphenolic contents and antioxidant activity evaluated by three radical scavenging assays. Using a microwell dilution method, the strongest antibacterial activity profiles were determined for ethanol and methanol root extracts (minimum bactericidal concentrations (MBCs) = 0.25-3.00 mg/mL), which were comparable to the activity of streptomycin (MBCs = 0.34-1.24 mg/mL), while the strongest antibacterial compound of A. pancicii was oxypeucedanin hydrate (MBCs = 0.50-8.00 mg/mL). Antifungal potential was in moderate extent, and the highest activity was obtained for root methanol extract (minimum fungicidal concentrations (MFCs) = 4.00-14.00 mg/mL). Tested sub-minimum inhibitory concentrations (subMICs) of the extracts and isolated compounds inhibited selected Pseudomonas aeruginosa PAO1 virulence determinants. The most reduced growth of P. aeruginosa colony was in the presence of isolated oxypeucedanin. Ethanol (17.36-46.98%) and methanol (34.54-52.43%) root extracts showed higher anti-biofilm activity compared to streptomycin (49.40-88.36%) and ampicillin (56.46-92.16%).
T2  - Journal of Agricultural and Food Chemistry
T1  - Research on Chemical Composition and Biological Properties Including Antiquorum Sensing Activity of Angelica pancicii Vandas Aerial Parts and Roots
IS  - 50
VL  - 65
DO  - 10.1021/acs.jafc.7b04202
SP  - 10933
EP  - 10949
ER  - 

@article{
author = "Mileski, Ksenija S. and Trifunović, Snežana S. and Ćirić, Ana and Šakić, Željana M. and Ristić, Mihailo S. and Todorović, Nina M. and Matevski, Vlado S. and Marin, Petar D. and Tešević, Vele V. and Džamić, Ana M.",
year = "2017",
abstract = "The essential oil, different extracts, and isolated compounds of Angelica pancicii Vandas (Apiaceae) were investigated for the first time. The GC-FID and GC-MS analyses revealed sesquiterpenoids as the main constituents of A. pancicii essential oil of aerial parts with bornyl acetate (8.08%), n-octanol (5.82%), kessane (4.26%), and β-selinene (4.26%) as the main constituents. Analysis of methanol extracts, using an HPLC-DAD/ESI-ToF-MS system, showed a total of 52 compounds in the aerial parts and 53 in the roots, indicating coumarins as the main constituents. In addition, new chromone (1) and six known furanocoumarins (2-7) were isolated from the roots and structurally elucidated by combined spectroscopic methods. The aerial part extracts exhibited higher polyphenolic contents and antioxidant activity evaluated by three radical scavenging assays. Using a microwell dilution method, the strongest antibacterial activity profiles were determined for ethanol and methanol root extracts (minimum bactericidal concentrations (MBCs) = 0.25-3.00 mg/mL), which were comparable to the activity of streptomycin (MBCs = 0.34-1.24 mg/mL), while the strongest antibacterial compound of A. pancicii was oxypeucedanin hydrate (MBCs = 0.50-8.00 mg/mL). Antifungal potential was in moderate extent, and the highest activity was obtained for root methanol extract (minimum fungicidal concentrations (MFCs) = 4.00-14.00 mg/mL). Tested sub-minimum inhibitory concentrations (subMICs) of the extracts and isolated compounds inhibited selected Pseudomonas aeruginosa PAO1 virulence determinants. The most reduced growth of P. aeruginosa colony was in the presence of isolated oxypeucedanin. Ethanol (17.36-46.98%) and methanol (34.54-52.43%) root extracts showed higher anti-biofilm activity compared to streptomycin (49.40-88.36%) and ampicillin (56.46-92.16%).",
journal = "Journal of Agricultural and Food Chemistry",
title = "Research on Chemical Composition and Biological Properties Including Antiquorum Sensing Activity of Angelica pancicii Vandas Aerial Parts and Roots",
number = "50",
volume = "65",
doi = "10.1021/acs.jafc.7b04202",
pages = "10933-10949"
}

Mileski, K. S., Trifunović, S. S., Ćirić, A., Šakić, Ž. M., Ristić, M. S., Todorović, N. M., Matevski, V. S., Marin, P. D., Tešević, V. V.,& Džamić, A. M.. (2017). Research on Chemical Composition and Biological Properties Including Antiquorum Sensing Activity of Angelica pancicii Vandas Aerial Parts and Roots. in Journal of Agricultural and Food Chemistry, 65(50), 10933-10949.
https://doi.org/10.1021/acs.jafc.7b04202

Mileski KS, Trifunović SS, Ćirić A, Šakić ŽM, Ristić MS, Todorović NM, Matevski VS, Marin PD, Tešević VV, Džamić AM. Research on Chemical Composition and Biological Properties Including Antiquorum Sensing Activity of Angelica pancicii Vandas Aerial Parts and Roots. in Journal of Agricultural and Food Chemistry. 2017;65(50):10933-10949.
doi:10.1021/acs.jafc.7b04202 .

Mileski, Ksenija S., Trifunović, Snežana S., Ćirić, Ana, Šakić, Željana M., Ristić, Mihailo S., Todorović, Nina M., Matevski, Vlado S., Marin, Petar D., Tešević, Vele V., Džamić, Ana M., "Research on Chemical Composition and Biological Properties Including Antiquorum Sensing Activity of Angelica pancicii Vandas Aerial Parts and Roots" in Journal of Agricultural and Food Chemistry, 65, no. 50 (2017):10933-10949,
https://doi.org/10.1021/acs.jafc.7b04202 . .

TY  - JOUR
AU  - Mileski, Ksenija S.
AU  - Džamić, Ana M.
AU  - Ćirić, Ana
AU  - Ristic, Mihailo S.
AU  - Grujic, Slavica M.
AU  - Matevski, Vlado S.
AU  - Marin, Petar D.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2083
AB  - Ferulago macedonica Micevski \& E. Mayer (Apiaceae), an endemic species
   from Macedonia, was evaluated for its essential oil composition and
   biological activity for the first time. The essential oil composition
   from inflorescence and aerial parts, analyzed by gas chromatography and
   gas chromatography-mass spectrometry (GC and GC-MS), indicated a-pinene
   (43.10\% and 22.78\%) and sabinene (26.73\% and 15.46\%) as the main
   components. The antioxidant activities of extracts was determinated
   spectrophotometrically using 2,2-diphenyl-1-picrylhydrazyl (DPPH). The
   strongest activity had methanol extracts of inflorescence and aerial
   parts (IC50= 490 mg/mL and IC50= 630 mg/mL of solution, respectively).
   According to 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid)
   (ABTS) test, the most effective were ethanol and methanol extracts of F.
   macedonica inflorescence (1.69 and 1.63 mg Vit.C/g dw). Total phenolic
   and flavonoid contents evaluation indicated that their amounts were
   ranged between 34.12-59.68 mg gallic acid (GA)/g dw for phenols and
   9.08-23.73 mg quercetin hydrate (QE)/g dw for flavonoids. Antimicrobial
   activity of extracts and essential oils was investigated by micro-well
   dilution assay. Minimum inhibitory (MIC) and minimum
   bactericidal/fungicidal concentrations (MBC/MFC) were defined. The
   methanol extract of inflorescence has shown to be the strongest in both,
   antibacterial (MICs= 4-8 mg/mL; MBCs= 9-13 mg/mL) and antifungal
   activity (MICs= 2-12 mg/mL; MFCs= 6-18 mg/mL). Essential oil from
   inflorescence and aerial parts of F. macedonica manifested stronger
   inhibition on growth of pathogenic bacteria and fungy compared to tested
   extracts and positive standards.
T2  - Records of Natural Products
T1  - Composition, Antimicrobial and Antioxidant Properties of Endemic Species
 Ferulago macedonica Micevski \& E. Mayer
IS  - 2
VL  - 9
SP  - 208
EP  - 223
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2083
ER  - 

@article{
author = "Mileski, Ksenija S. and Džamić, Ana M. and Ćirić, Ana and Ristic, Mihailo S. and Grujic, Slavica M. and Matevski, Vlado S. and Marin, Petar D.",
year = "2015",
abstract = "Ferulago macedonica Micevski \& E. Mayer (Apiaceae), an endemic species
   from Macedonia, was evaluated for its essential oil composition and
   biological activity for the first time. The essential oil composition
   from inflorescence and aerial parts, analyzed by gas chromatography and
   gas chromatography-mass spectrometry (GC and GC-MS), indicated a-pinene
   (43.10\% and 22.78\%) and sabinene (26.73\% and 15.46\%) as the main
   components. The antioxidant activities of extracts was determinated
   spectrophotometrically using 2,2-diphenyl-1-picrylhydrazyl (DPPH). The
   strongest activity had methanol extracts of inflorescence and aerial
   parts (IC50= 490 mg/mL and IC50= 630 mg/mL of solution, respectively).
   According to 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid)
   (ABTS) test, the most effective were ethanol and methanol extracts of F.
   macedonica inflorescence (1.69 and 1.63 mg Vit.C/g dw). Total phenolic
   and flavonoid contents evaluation indicated that their amounts were
   ranged between 34.12-59.68 mg gallic acid (GA)/g dw for phenols and
   9.08-23.73 mg quercetin hydrate (QE)/g dw for flavonoids. Antimicrobial
   activity of extracts and essential oils was investigated by micro-well
   dilution assay. Minimum inhibitory (MIC) and minimum
   bactericidal/fungicidal concentrations (MBC/MFC) were defined. The
   methanol extract of inflorescence has shown to be the strongest in both,
   antibacterial (MICs= 4-8 mg/mL; MBCs= 9-13 mg/mL) and antifungal
   activity (MICs= 2-12 mg/mL; MFCs= 6-18 mg/mL). Essential oil from
   inflorescence and aerial parts of F. macedonica manifested stronger
   inhibition on growth of pathogenic bacteria and fungy compared to tested
   extracts and positive standards.",
journal = "Records of Natural Products",
title = "Composition, Antimicrobial and Antioxidant Properties of Endemic Species
 Ferulago macedonica Micevski \& E. Mayer",
number = "2",
volume = "9",
pages = "208-223",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2083"
}

Mileski, K. S., Džamić, A. M., Ćirić, A., Ristic, M. S., Grujic, S. M., Matevski, V. S.,& Marin, P. D.. (2015). Composition, Antimicrobial and Antioxidant Properties of Endemic Species
 Ferulago macedonica Micevski \& E. Mayer. in Records of Natural Products, 9(2), 208-223.
https://hdl.handle.net/21.15107/rcub_ibiss_2083

Mileski KS, Džamić AM, Ćirić A, Ristic MS, Grujic SM, Matevski VS, Marin PD. Composition, Antimicrobial and Antioxidant Properties of Endemic Species
 Ferulago macedonica Micevski \& E. Mayer. in Records of Natural Products. 2015;9(2):208-223.
https://hdl.handle.net/21.15107/rcub_ibiss_2083 .

Mileski, Ksenija S., Džamić, Ana M., Ćirić, Ana, Ristic, Mihailo S., Grujic, Slavica M., Matevski, Vlado S., Marin, Petar D., "Composition, Antimicrobial and Antioxidant Properties of Endemic Species
 Ferulago macedonica Micevski \& E. Mayer" in Records of Natural Products, 9, no. 2 (2015):208-223,
https://hdl.handle.net/21.15107/rcub_ibiss_2083 .