TY  - JOUR
AU  - Dakić, Tamara
AU  - Jevđović, Tanja
AU  - Vujović, Predrag
AU  - Mladenović, Aleksandra
PY  - 2022
UR  - https://www.mdpi.com/1422-0067/23/12/6546
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5018
AB  - Striving for longevity is neither a recent human desire nor a novel scientific field. The first article on this topic was published in 1838, when the average human life expectancy was approximately 40 years. Although nowadays people on average live almost as twice as long, we still (and perhaps more than ever) look for new ways to extend our lifespan. During this seemingly endless journey of discovering efficient methods to prolong life, humans were enthusiastic regarding several approaches, one of which is caloric restriction (CR). Where does CR, initially considered universally beneficial for extending both lifespan and health span, stand today? Does a lifelong decrease in food consumption represent one of the secrets of centenarians' long and healthy life? Do we still believe that if we eat less, we will live longer? This review aims to summarize the current literature on CR as a potential life-prolonging intervention in humans and discusses metabolic pathways that underlie this effect.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - The Less We Eat, the Longer We Live: Can Caloric Restriction Help Us Become Centenarians?
IS  - 12
VL  - 23
DO  - 10.3390/ijms23126546
SP  - 6546
ER  - 

@article{
author = "Dakić, Tamara and Jevđović, Tanja and Vujović, Predrag and Mladenović, Aleksandra",
year = "2022",
abstract = "Striving for longevity is neither a recent human desire nor a novel scientific field. The first article on this topic was published in 1838, when the average human life expectancy was approximately 40 years. Although nowadays people on average live almost as twice as long, we still (and perhaps more than ever) look for new ways to extend our lifespan. During this seemingly endless journey of discovering efficient methods to prolong life, humans were enthusiastic regarding several approaches, one of which is caloric restriction (CR). Where does CR, initially considered universally beneficial for extending both lifespan and health span, stand today? Does a lifelong decrease in food consumption represent one of the secrets of centenarians' long and healthy life? Do we still believe that if we eat less, we will live longer? This review aims to summarize the current literature on CR as a potential life-prolonging intervention in humans and discusses metabolic pathways that underlie this effect.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "The Less We Eat, the Longer We Live: Can Caloric Restriction Help Us Become Centenarians?",
number = "12",
volume = "23",
doi = "10.3390/ijms23126546",
pages = "6546"
}

Dakić, T., Jevđović, T., Vujović, P.,& Mladenović, A.. (2022). The Less We Eat, the Longer We Live: Can Caloric Restriction Help Us Become Centenarians?. in International Journal of Molecular Sciences
Basel: MDPI., 23(12), 6546.
https://doi.org/10.3390/ijms23126546

Dakić T, Jevđović T, Vujović P, Mladenović A. The Less We Eat, the Longer We Live: Can Caloric Restriction Help Us Become Centenarians?. in International Journal of Molecular Sciences. 2022;23(12):6546.
doi:10.3390/ijms23126546 .

Dakić, Tamara, Jevđović, Tanja, Vujović, Predrag, Mladenović, Aleksandra, "The Less We Eat, the Longer We Live: Can Caloric Restriction Help Us Become Centenarians?" in International Journal of Molecular Sciences, 23, no. 12 (2022):6546,
https://doi.org/10.3390/ijms23126546 . .

TY  - JOUR
AU  - Trifunović, Svetlana
AU  - Lakić, Iva
AU  - Vujović, Predrag
AU  - Jevđović, Tanja
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
AU  - Đorđević, Jelena
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0065128118301041?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3159
AB  - It is well known that stress changes levels of pituitary hormones in the bloodstream and in the pituitary itself. However, almost nothing is known about the impact of stress on histological and stereological parameters of the growth hormone producing cells (somatotrophs-GH cells). The aim of the present study was to investigate the effect of: acute and repeated immobilization; acute and repeated restraint on histological and morphofunctional parameters of somatotrophs in adult Wistar rats. Changes in the pituitary gland volume; the volume density and volume of somatotrophs following acute and repeated immobilization (IMO, R-IMO); acute and repeated restraint (R, R-R) were evaluated using a stereological system (newCAST), while growth hormone level within pituitary was determined by Western blot. Our results demonstrated the decrease (p < 0.05) of the pituitary volume (17%, 19%) in the IMO and R groups, respectively, and the increase in the R-R group. The volume density of GH cells decreased (p < 0.05) in the R-IMO (7%), R (26%) and R-R (18%) group in comparison to the control value. The pituitary GH content was increased (p < 0.05) after the IMO (2-fold), R (2.5-fold) and R-R (2.1-fold) as compared to the control group. These results point out that acute and repeated immobilization and/or restraint lead not only to changes in GH hormone concentration, but also modify the morphological aspects of GH cells within the rat pituitary.
T2  - Acta histochemica
T1  - Morphofunctional parameters of rat somatotrophes after acute and repeated immobilization or restraint stress.
IS  - 1
VL  - 121
DO  - 10.1016/j.acthis.2018.10.003
SP  - 29
EP  - 34
ER  - 

@article{
author = "Trifunović, Svetlana and Lakić, Iva and Vujović, Predrag and Jevđović, Tanja and Šošić-Jurjević, Branka  and Milošević, Verica and Đorđević, Jelena",
year = "2019",
abstract = "It is well known that stress changes levels of pituitary hormones in the bloodstream and in the pituitary itself. However, almost nothing is known about the impact of stress on histological and stereological parameters of the growth hormone producing cells (somatotrophs-GH cells). The aim of the present study was to investigate the effect of: acute and repeated immobilization; acute and repeated restraint on histological and morphofunctional parameters of somatotrophs in adult Wistar rats. Changes in the pituitary gland volume; the volume density and volume of somatotrophs following acute and repeated immobilization (IMO, R-IMO); acute and repeated restraint (R, R-R) were evaluated using a stereological system (newCAST), while growth hormone level within pituitary was determined by Western blot. Our results demonstrated the decrease (p < 0.05) of the pituitary volume (17%, 19%) in the IMO and R groups, respectively, and the increase in the R-R group. The volume density of GH cells decreased (p < 0.05) in the R-IMO (7%), R (26%) and R-R (18%) group in comparison to the control value. The pituitary GH content was increased (p < 0.05) after the IMO (2-fold), R (2.5-fold) and R-R (2.1-fold) as compared to the control group. These results point out that acute and repeated immobilization and/or restraint lead not only to changes in GH hormone concentration, but also modify the morphological aspects of GH cells within the rat pituitary.",
journal = "Acta histochemica",
title = "Morphofunctional parameters of rat somatotrophes after acute and repeated immobilization or restraint stress.",
number = "1",
volume = "121",
doi = "10.1016/j.acthis.2018.10.003",
pages = "29-34"
}

Trifunović, S., Lakić, I., Vujović, P., Jevđović, T., Šošić-Jurjević, B., Milošević, V.,& Đorđević, J.. (2019). Morphofunctional parameters of rat somatotrophes after acute and repeated immobilization or restraint stress.. in Acta histochemica, 121(1), 29-34.
https://doi.org/10.1016/j.acthis.2018.10.003

Trifunović S, Lakić I, Vujović P, Jevđović T, Šošić-Jurjević B, Milošević V, Đorđević J. Morphofunctional parameters of rat somatotrophes after acute and repeated immobilization or restraint stress.. in Acta histochemica. 2019;121(1):29-34.
doi:10.1016/j.acthis.2018.10.003 .

Trifunović, Svetlana, Lakić, Iva, Vujović, Predrag, Jevđović, Tanja, Šošić-Jurjević, Branka , Milošević, Verica, Đorđević, Jelena, "Morphofunctional parameters of rat somatotrophes after acute and repeated immobilization or restraint stress." in Acta histochemica, 121, no. 1 (2019):29-34,
https://doi.org/10.1016/j.acthis.2018.10.003 . .

TY  - CONF
AU  - Đurašević, Siniša
AU  - Jasnić, Nebojša
AU  - Dakić, Tamara
AU  - Jevđović, Tanja
AU  - Lakić, Iva
AU  - Vujović, Predrag
AU  - Đorđević, Jelena
AU  - Mitić-Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Pavlović, Slađan
AU  - Prokić, Marko
AU  - Zaletel, Ivan
AU  - Todorović, Zoran
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3422
AB  - We investigated the effect of long-term high-dose virgin coconut oil (VCO) supplementation on rat glucose homeostasis. Animals were divided into two groups with 6 of them in each: normally fed (Control group) and the group fed with coconut oil at a concentration of 20% in food (VCO group). The experiment lasted for four months. We measured fasting glycemia once a week during the entire experiment. In the last week of the experiment, we performed an oral glucose tolerance test (OGTT) and an intraperitoneal insulin tolerance test (ITT). On the last day of the experiment the fasting insulin and glyc8mia were measured in the blood of animals. The results show that coconut oil reduces weekly glycemia in VCO animals compared with controls. This effect reaches its maximum after the first two weeks of the experiment, and then slowly decreases and disappears over time of next eight weeks. As a result, the glycemia of control and VCO animals do not differ in last six weeks of the experiment. The area under the curve (AUC) presenting glycemia during whole the experiment is significantly lower in VCO animals than in the controls. The hypoglycemic effect of coconut oil is obviously dose-dependent since the amount of food (and therefore the coconut oil) that the animals eat decreases over the time. The results of the oral glucose tolerance test show that the OGTT AUC of VCO animals is significantly lower than the controls, and same is true for the insulin tolerance test. Finally, glycemia and insulin concentration in serums sampled on the last day of the experiment do not differ between VCO and Control groups, so accordingly neither HOMA-IR I and 2 (Homeostatic model assessment of insulin resistance) nor QUIC.Kl ( Quantitative Insulin Sensitivity Check Index). In conclusion, our results show beneficial effects of long-term high-dose coconut oil supplementation on rat glucose homeostasis.
PB  - BIT Congress Inc. (BIT Group Global Ltd.)
C3  - BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017
T1  - The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis
SP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3422
ER  - 

@conference{
author = "Đurašević, Siniša and Jasnić, Nebojša and Dakić, Tamara and Jevđović, Tanja and Lakić, Iva and Vujović, Predrag and Đorđević, Jelena and Mitić-Ćulafić, Dragana and Nikolić, Biljana and Grigorov, Ilijana and Bogojević, Desanka and Pavlović, Slađan and Prokić, Marko and Zaletel, Ivan and Todorović, Zoran",
year = "2017",
abstract = "We investigated the effect of long-term high-dose virgin coconut oil (VCO) supplementation on rat glucose homeostasis. Animals were divided into two groups with 6 of them in each: normally fed (Control group) and the group fed with coconut oil at a concentration of 20% in food (VCO group). The experiment lasted for four months. We measured fasting glycemia once a week during the entire experiment. In the last week of the experiment, we performed an oral glucose tolerance test (OGTT) and an intraperitoneal insulin tolerance test (ITT). On the last day of the experiment the fasting insulin and glyc8mia were measured in the blood of animals. The results show that coconut oil reduces weekly glycemia in VCO animals compared with controls. This effect reaches its maximum after the first two weeks of the experiment, and then slowly decreases and disappears over time of next eight weeks. As a result, the glycemia of control and VCO animals do not differ in last six weeks of the experiment. The area under the curve (AUC) presenting glycemia during whole the experiment is significantly lower in VCO animals than in the controls. The hypoglycemic effect of coconut oil is obviously dose-dependent since the amount of food (and therefore the coconut oil) that the animals eat decreases over the time. The results of the oral glucose tolerance test show that the OGTT AUC of VCO animals is significantly lower than the controls, and same is true for the insulin tolerance test. Finally, glycemia and insulin concentration in serums sampled on the last day of the experiment do not differ between VCO and Control groups, so accordingly neither HOMA-IR I and 2 (Homeostatic model assessment of insulin resistance) nor QUIC.Kl ( Quantitative Insulin Sensitivity Check Index). In conclusion, our results show beneficial effects of long-term high-dose coconut oil supplementation on rat glucose homeostasis.",
publisher = "BIT Congress Inc. (BIT Group Global Ltd.)",
journal = "BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017",
title = "The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis",
pages = "167",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3422"
}

Đurašević, S., Jasnić, N., Dakić, T., Jevđović, T., Lakić, I., Vujović, P., Đorđević, J., Mitić-Ćulafić, D., Nikolić, B., Grigorov, I., Bogojević, D., Pavlović, S., Prokić, M., Zaletel, I.,& Todorović, Z.. (2017). The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis. in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017
BIT Congress Inc. (BIT Group Global Ltd.)., 167.
https://hdl.handle.net/21.15107/rcub_ibiss_3422

Đurašević S, Jasnić N, Dakić T, Jevđović T, Lakić I, Vujović P, Đorđević J, Mitić-Ćulafić D, Nikolić B, Grigorov I, Bogojević D, Pavlović S, Prokić M, Zaletel I, Todorović Z. The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis. in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017. 2017;:167.
https://hdl.handle.net/21.15107/rcub_ibiss_3422 .

Đurašević, Siniša, Jasnić, Nebojša, Dakić, Tamara, Jevđović, Tanja, Lakić, Iva, Vujović, Predrag, Đorđević, Jelena, Mitić-Ćulafić, Dragana, Nikolić, Biljana, Grigorov, Ilijana, Bogojević, Desanka, Pavlović, Slađan, Prokić, Marko, Zaletel, Ivan, Todorović, Zoran, "The effect of long-term high-dose coconut oil supplementation on rat glucose homeostasis" in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017 (2017):167,
https://hdl.handle.net/21.15107/rcub_ibiss_3422 .

TY  - CONF
AU  - Đurašević, Siniša
AU  - Jasnić, Nebojša
AU  - Dakić, Tamara
AU  - Jevđović, Tanja
AU  - Lakić, Iva
AU  - Vujović, Predrag
AU  - Đorđević, Jelena
AU  - Mitić-Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Grigorov, Ilijana
AU  - Bogojević, Desanka
AU  - Pavlović, Slađan
AU  - Prokić, Marko
AU  - Zaletel, Ivan
AU  - Todorović, Zoran
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3423
AB  - We investigated the effect of long-term high-dose virgin coconut oil (VCO) supplementation on rat liver and serum lipid status. Animals were divided into two groups with 8 of them in each: normally fed (Control group) and the group fed with coconut oil at a concentration of 20% in food (VCO group). The experiment lasted for four months. On the last day of the experiment animals were killed, and blood and liver tissue were collected. In serum we measured the levels of total cholesterol (TC), high-density lipoproteins (HDL), non-HDL lipoproteins, triglycerides (TG), aspartate aminotransferase (9\.ST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). We also measured both liver and serum levels of high mobility group protein B 1 (HMGB 1) and haptoglobin (HP), as ,.vell as the liver level of NF-KB p65/ p-NF-KB p65 transcription factor, together with the histopathology analysis on liver slices and liver Comet assay. The results show that coconut oil do not change serum TC and HDL, but reduces non-HDL and TG levels (10% and 50%, respectively) comparing to control. As a result, atherogenic index of serum (AI) is strongly reduced in VCO group versus control. As for the liver status, results show that coconut supplementation increases AST, ALT and ALP levels in VCO group (50%, 30% and 60%, respectively) comparing to control. This effect is caused by the accumulation of coconut oil fat in liver, as confirmed by the histopathology showing signs of mild nonalcoholic steatohepatitis in VCO group, followed with the increased %of DNA in comet tail. The liver inflammation in VCO group is further demonstrated with the liver HP, HMGBl and p-NF-KB p65 level increase, and increase in nuclear level ofNF­kB p65, but not accompanying serum HP and HMGBl increase. In conclusion, our results show that coconut oil supplementation, despite causing mild and localized steatohepatitis, also lowers serum atherogcnic index, a predictor of cardiovascular risk.
PB  - BIT Congress Inc. (BIT Group Global Ltd.)
C3  - BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017
T1  - The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids
SP  - 168
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3423
ER  - 

@conference{
author = "Đurašević, Siniša and Jasnić, Nebojša and Dakić, Tamara and Jevđović, Tanja and Lakić, Iva and Vujović, Predrag and Đorđević, Jelena and Mitić-Ćulafić, Dragana and Nikolić, Biljana and Grigorov, Ilijana and Bogojević, Desanka and Pavlović, Slađan and Prokić, Marko and Zaletel, Ivan and Todorović, Zoran",
year = "2017",
abstract = "We investigated the effect of long-term high-dose virgin coconut oil (VCO) supplementation on rat liver and serum lipid status. Animals were divided into two groups with 8 of them in each: normally fed (Control group) and the group fed with coconut oil at a concentration of 20% in food (VCO group). The experiment lasted for four months. On the last day of the experiment animals were killed, and blood and liver tissue were collected. In serum we measured the levels of total cholesterol (TC), high-density lipoproteins (HDL), non-HDL lipoproteins, triglycerides (TG), aspartate aminotransferase (9\.ST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). We also measured both liver and serum levels of high mobility group protein B 1 (HMGB 1) and haptoglobin (HP), as ,.vell as the liver level of NF-KB p65/ p-NF-KB p65 transcription factor, together with the histopathology analysis on liver slices and liver Comet assay. The results show that coconut oil do not change serum TC and HDL, but reduces non-HDL and TG levels (10% and 50%, respectively) comparing to control. As a result, atherogenic index of serum (AI) is strongly reduced in VCO group versus control. As for the liver status, results show that coconut supplementation increases AST, ALT and ALP levels in VCO group (50%, 30% and 60%, respectively) comparing to control. This effect is caused by the accumulation of coconut oil fat in liver, as confirmed by the histopathology showing signs of mild nonalcoholic steatohepatitis in VCO group, followed with the increased %of DNA in comet tail. The liver inflammation in VCO group is further demonstrated with the liver HP, HMGBl and p-NF-KB p65 level increase, and increase in nuclear level ofNF­kB p65, but not accompanying serum HP and HMGBl increase. In conclusion, our results show that coconut oil supplementation, despite causing mild and localized steatohepatitis, also lowers serum atherogcnic index, a predictor of cardiovascular risk.",
publisher = "BIT Congress Inc. (BIT Group Global Ltd.)",
journal = "BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017",
title = "The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids",
pages = "168",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3423"
}

Đurašević, S., Jasnić, N., Dakić, T., Jevđović, T., Lakić, I., Vujović, P., Đorđević, J., Mitić-Ćulafić, D., Nikolić, B., Grigorov, I., Bogojević, D., Pavlović, S., Prokić, M., Zaletel, I.,& Todorović, Z.. (2017). The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids. in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017
BIT Congress Inc. (BIT Group Global Ltd.)., 168.
https://hdl.handle.net/21.15107/rcub_ibiss_3423

Đurašević S, Jasnić N, Dakić T, Jevđović T, Lakić I, Vujović P, Đorđević J, Mitić-Ćulafić D, Nikolić B, Grigorov I, Bogojević D, Pavlović S, Prokić M, Zaletel I, Todorović Z. The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids. in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017. 2017;:168.
https://hdl.handle.net/21.15107/rcub_ibiss_3423 .

Đurašević, Siniša, Jasnić, Nebojša, Dakić, Tamara, Jevđović, Tanja, Lakić, Iva, Vujović, Predrag, Đorđević, Jelena, Mitić-Ćulafić, Dragana, Nikolić, Biljana, Grigorov, Ilijana, Bogojević, Desanka, Pavlović, Slađan, Prokić, Marko, Zaletel, Ivan, Todorović, Zoran, "The effect of long-term high-dose coconut oil supplementation on rat sliver and serum lipids" in BIT´s 6th Annual World Congress of Food and Nutrition: Abstract Book. Shenyang, China; September 15-17, 2017 (2017):168,
https://hdl.handle.net/21.15107/rcub_ibiss_3423 .