TY  - JOUR
AU  - Li, Hanluo
AU  - Ziemer, Mirjana
AU  - Stojanović, Ivana D.
AU  - Saksida, Tamara
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Đmura, Goran
AU  - Mićanović, Dragica
AU  - Koprivica, Ivan
AU  - Krajnović, Tamara
AU  - Drača, Dijana
AU  - Simon, Jan-Christoph
AU  - Lethaus, Bernd
AU  - Savković, Vuk
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4775
AB  - Wound  healing  of  acute  full-thickness  injuries  and  chronic  non-healing  ulcers  leads  to  delayed  wound  closure,  prolonged recovery period and hypertrophic scarring, generating a demand for an autologous cell therapy and a relevant pre-clinical research  models for  wound healing.  In this study, an  immunocompetent model  for  wound  healing  was  employed  using a syngeneic murine cell line of mesenchymal stem cells cultured from the mouse whisker hair follicle outer root sheath (named moMSCORS). moMSCORS were isolated using an air-liquid interface method, expanded  in vitro  and characterized according to the MSC definition criteria - cell viability,  in vitro  proliferation, MSC phenotype and multi-lineage differentiations. Moreover, upon applying moMSCORS in an  in vivo  full-thickness wound model in the syngeneic C57BL/6 mice, the treated wounds displayed different morphology to that of the untreated wound beds. Quantitative evaluation of angiogenesis, granulation and wound closure involving clinical scoring and software-based quantification indicated a lower degree of inflammation in the treated wounds. Histological staining of treated wounds by the means of H&E, Alcian Blue, PicroSirius Red and αSMA immune labelling showed lower cellularity, less collagen filaments as well as thinner dermal and epidermal layers compared with the untreated wounds, indicating a general reduction of hypertrophic scars. The decreased inflammation, accelerated wound closure and non-hypertrophic scarring, which were facilitated by moMSCORS, hereby address a common problem of hypertrophic scars and non-physiological tissue properties upon wound closure, and additionally offer an  in  vivo  model  for  the  autologous  cell-based  wound  healing.
PB  - Basel: Springer Nature Switzerland AG
T2  - Stem Cell Reviews and Reports
T1  - Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine  Wound Healing Model
DO  - 10.1007/s12015-021-10288-7
ER  - 

@article{
author = "Li, Hanluo and Ziemer, Mirjana and Stojanović, Ivana D. and Saksida, Tamara and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Đmura, Goran and Mićanović, Dragica and Koprivica, Ivan and Krajnović, Tamara and Drača, Dijana and Simon, Jan-Christoph and Lethaus, Bernd and Savković, Vuk",
year = "2022",
abstract = "Wound  healing  of  acute  full-thickness  injuries  and  chronic  non-healing  ulcers  leads  to  delayed  wound  closure,  prolonged recovery period and hypertrophic scarring, generating a demand for an autologous cell therapy and a relevant pre-clinical research  models for  wound healing.  In this study, an  immunocompetent model  for  wound  healing  was  employed  using a syngeneic murine cell line of mesenchymal stem cells cultured from the mouse whisker hair follicle outer root sheath (named moMSCORS). moMSCORS were isolated using an air-liquid interface method, expanded  in vitro  and characterized according to the MSC definition criteria - cell viability,  in vitro  proliferation, MSC phenotype and multi-lineage differentiations. Moreover, upon applying moMSCORS in an  in vivo  full-thickness wound model in the syngeneic C57BL/6 mice, the treated wounds displayed different morphology to that of the untreated wound beds. Quantitative evaluation of angiogenesis, granulation and wound closure involving clinical scoring and software-based quantification indicated a lower degree of inflammation in the treated wounds. Histological staining of treated wounds by the means of H&E, Alcian Blue, PicroSirius Red and αSMA immune labelling showed lower cellularity, less collagen filaments as well as thinner dermal and epidermal layers compared with the untreated wounds, indicating a general reduction of hypertrophic scars. The decreased inflammation, accelerated wound closure and non-hypertrophic scarring, which were facilitated by moMSCORS, hereby address a common problem of hypertrophic scars and non-physiological tissue properties upon wound closure, and additionally offer an  in  vivo  model  for  the  autologous  cell-based  wound  healing.",
publisher = "Basel: Springer Nature Switzerland AG",
journal = "Stem Cell Reviews and Reports",
title = "Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine  Wound Healing Model",
doi = "10.1007/s12015-021-10288-7"
}

Li, H., Ziemer, M., Stojanović, I. D., Saksida, T., Maksimović-Ivanić, D., Mijatović, S., Đmura, G., Mićanović, D., Koprivica, I., Krajnović, T., Drača, D., Simon, J., Lethaus, B.,& Savković, V.. (2022). Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine  Wound Healing Model. in Stem Cell Reviews and Reports
Basel: Springer Nature Switzerland AG..
https://doi.org/10.1007/s12015-021-10288-7

Li H, Ziemer M, Stojanović ID, Saksida T, Maksimović-Ivanić D, Mijatović S, Đmura G, Mićanović D, Koprivica I, Krajnović T, Drača D, Simon J, Lethaus B, Savković V. Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine  Wound Healing Model. in Stem Cell Reviews and Reports. 2022;.
doi:10.1007/s12015-021-10288-7 .

Li, Hanluo, Ziemer, Mirjana, Stojanović, Ivana D., Saksida, Tamara, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Đmura, Goran, Mićanović, Dragica, Koprivica, Ivan, Krajnović, Tamara, Drača, Dijana, Simon, Jan-Christoph, Lethaus, Bernd, Savković, Vuk, "Mesenchymal Stem Cells From Mouse Hair Follicles Reduce Hypertrophic Scarring in a Murine  Wound Healing Model" in Stem Cell Reviews and Reports (2022),
https://doi.org/10.1007/s12015-021-10288-7 . .

TY  - JOUR
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Srbovan, Maja
AU  - Đmura, Goran
AU  - Pešić, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5336
AB  - Changes in housing density, including individual housing, are commonly necessary in animal research. Obtaining reproducibility and translational validity in biomedical research requires an understanding of how animals adapt to changes in housing density. Existing literature mainly addresses acclimatization after transportation. We used a within-subject design to examine changes in behavior and weight gain of 4-mo-old male Wistar Han rats after reduction of their social group (RSG; due to removal of one rat from a cage containing 3 rats) and social isolation (SI; the removed rat) for the subsequent 2 wk. Changes in weight gain and in exploratory and center-avoidance behavior in an inescapable open arena (OA) were measured before (D0) and on days 7 and 14 (D7 and D14, respectively) after social change. The motor response to d-amphetamine (1.5 mg/kg), which stimulates behavioral arousal in response to novelty, was assessed at D14. Within-subject design revealed that RSG rats in OA had less locomotion at D7 but not more center-avoidance behavior and had returned to the D0 activity level at D14; SI rats in OA had consistently less locomotion and more center-avoidance behavior. Rearing behavior during OA exposure did not change in either group. However, SI rats showed more center-avoidance behavior in OA, greater weight gain, and less amphetamine-induced rearing at D14 as compared with RSG rats. These data indicate that after RSG, mature adult male rats require 2 wk to return to their baseline level of OA-related behavior, while after SI they gain weight and acquire maladaptive exploratory and center-avoidance behavior. The finding that SI produces maladaptive behavioral and physiologic alterations in adult male rats deserves attention because these changes could have confounding effects on research findings.
PB  - Memphis: American Association for Laboratory Animal Science
T2  - Journal of the American Association for Laboratory Animal Science
T1  - Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation
IS  - 6
VL  - 61
DO  - 10.30802/AALAS-JAALAS-22-000032
SP  - 615
EP  - 623
ER  - 

@article{
author = "Potrebić, Milica and Pavković, Željko and Srbovan, Maja and Đmura, Goran and Pešić, Vesna",
year = "2022",
abstract = "Changes in housing density, including individual housing, are commonly necessary in animal research. Obtaining reproducibility and translational validity in biomedical research requires an understanding of how animals adapt to changes in housing density. Existing literature mainly addresses acclimatization after transportation. We used a within-subject design to examine changes in behavior and weight gain of 4-mo-old male Wistar Han rats after reduction of their social group (RSG; due to removal of one rat from a cage containing 3 rats) and social isolation (SI; the removed rat) for the subsequent 2 wk. Changes in weight gain and in exploratory and center-avoidance behavior in an inescapable open arena (OA) were measured before (D0) and on days 7 and 14 (D7 and D14, respectively) after social change. The motor response to d-amphetamine (1.5 mg/kg), which stimulates behavioral arousal in response to novelty, was assessed at D14. Within-subject design revealed that RSG rats in OA had less locomotion at D7 but not more center-avoidance behavior and had returned to the D0 activity level at D14; SI rats in OA had consistently less locomotion and more center-avoidance behavior. Rearing behavior during OA exposure did not change in either group. However, SI rats showed more center-avoidance behavior in OA, greater weight gain, and less amphetamine-induced rearing at D14 as compared with RSG rats. These data indicate that after RSG, mature adult male rats require 2 wk to return to their baseline level of OA-related behavior, while after SI they gain weight and acquire maladaptive exploratory and center-avoidance behavior. The finding that SI produces maladaptive behavioral and physiologic alterations in adult male rats deserves attention because these changes could have confounding effects on research findings.",
publisher = "Memphis: American Association for Laboratory Animal Science",
journal = "Journal of the American Association for Laboratory Animal Science",
title = "Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation",
number = "6",
volume = "61",
doi = "10.30802/AALAS-JAALAS-22-000032",
pages = "615-623"
}

Potrebić, M., Pavković, Ž., Srbovan, M., Đmura, G.,& Pešić, V.. (2022). Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation. in Journal of the American Association for Laboratory Animal Science
Memphis: American Association for Laboratory Animal Science., 61(6), 615-623.
https://doi.org/10.30802/AALAS-JAALAS-22-000032

Potrebić M, Pavković Ž, Srbovan M, Đmura G, Pešić V. Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation. in Journal of the American Association for Laboratory Animal Science. 2022;61(6):615-623.
doi:10.30802/AALAS-JAALAS-22-000032 .

Potrebić, Milica, Pavković, Željko, Srbovan, Maja, Đmura, Goran, Pešić, Vesna, "Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation" in Journal of the American Association for Laboratory Animal Science, 61, no. 6 (2022):615-623,
https://doi.org/10.30802/AALAS-JAALAS-22-000032 . .

TY  - JOUR
AU  - Drača, Dijana
AU  - Edeler, David
AU  - Saoud, Mohamad
AU  - Dojčinović, Biljana
AU  - Dunđerović, Duško
AU  - Đmura, Goran
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Kaluđerović, Goran N.
PY  - 2021
UR  - internal-pdf://Drača et al. - 2021 - Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells i.pdf
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0162013421000301
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33582397
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4154
AB  - CP (cisplatin) and mesoporous silica SBA-15 (Santa Barbara amorphous 15) loaded with CP (→SBA-15|CP) were tested in vitro and in vivo against low metastatic mouse melanoma B16F1 cell line. SBA-15 only, as drug carrier, is found to be not active, while CP and SBA-15|CP revealed high cytotoxicity in lower μM range. The activity of SBA-15|CP was found similar to the activity of CP alone. Both CP and SBA-15|CP induced inhibition of cell proliferation (carboxyfluorescein succinimidyl ester - CFSE assay) along with G2/M arrest (4',6-diamidino-2-phenylindole - DAPI assay). Apoptosis (Annexin V/ propidium iodide - PI assay), through caspase activation (apostat assay) and nitric oxide (NO) production (diacetate(4-amino-5-methylamino-2',7'-difluorofluorescein-diacetat) - DAF FM assay), was identified as main mode of cell death. However, slight elevated autophagy (acridine orange - AO assay) was detected in treated B16F1 cells. CP and SBA-15|CP did not affect production of ROS (reactive oxygen species) in B16F1 cells. Both SBA-15|CP and CP induced in B16F1 G2 arrest and subsequent senescence. SBA-15|CP, but not CP, blocked the growth of melanoma in C57BL/6 mice. Moreover, hepato- and nephrotoxicity in SBA-15|CP treated animals were diminished in comparison to CP confirming multiply improved antitumor potential of immobilized CP. Outstandingly, SBA-15 boosted in vivo activity and diminished side effects of CP.
PB  - Elsevier BV
T2  - Journal of Inorganic Biochemistry
T1  - Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.
VL  - 217
DO  - 10.1016/j.jinorgbio.2021.111383
SP  - 111383
ER  - 

@article{
author = "Drača, Dijana and Edeler, David and Saoud, Mohamad and Dojčinović, Biljana and Dunđerović, Duško and Đmura, Goran and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Kaluđerović, Goran N.",
year = "2021",
abstract = "CP (cisplatin) and mesoporous silica SBA-15 (Santa Barbara amorphous 15) loaded with CP (→SBA-15|CP) were tested in vitro and in vivo against low metastatic mouse melanoma B16F1 cell line. SBA-15 only, as drug carrier, is found to be not active, while CP and SBA-15|CP revealed high cytotoxicity in lower μM range. The activity of SBA-15|CP was found similar to the activity of CP alone. Both CP and SBA-15|CP induced inhibition of cell proliferation (carboxyfluorescein succinimidyl ester - CFSE assay) along with G2/M arrest (4',6-diamidino-2-phenylindole - DAPI assay). Apoptosis (Annexin V/ propidium iodide - PI assay), through caspase activation (apostat assay) and nitric oxide (NO) production (diacetate(4-amino-5-methylamino-2',7'-difluorofluorescein-diacetat) - DAF FM assay), was identified as main mode of cell death. However, slight elevated autophagy (acridine orange - AO assay) was detected in treated B16F1 cells. CP and SBA-15|CP did not affect production of ROS (reactive oxygen species) in B16F1 cells. Both SBA-15|CP and CP induced in B16F1 G2 arrest and subsequent senescence. SBA-15|CP, but not CP, blocked the growth of melanoma in C57BL/6 mice. Moreover, hepato- and nephrotoxicity in SBA-15|CP treated animals were diminished in comparison to CP confirming multiply improved antitumor potential of immobilized CP. Outstandingly, SBA-15 boosted in vivo activity and diminished side effects of CP.",
publisher = "Elsevier BV",
journal = "Journal of Inorganic Biochemistry",
title = "Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.",
volume = "217",
doi = "10.1016/j.jinorgbio.2021.111383",
pages = "111383"
}

Drača, D., Edeler, D., Saoud, M., Dojčinović, B., Dunđerović, D., Đmura, G., Maksimović-Ivanić, D., Mijatović, S.,& Kaluđerović, G. N.. (2021). Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.. in Journal of Inorganic Biochemistry
Elsevier BV., 217, 111383.
https://doi.org/10.1016/j.jinorgbio.2021.111383

Drača D, Edeler D, Saoud M, Dojčinović B, Dunđerović D, Đmura G, Maksimović-Ivanić D, Mijatović S, Kaluđerović GN. Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies.. in Journal of Inorganic Biochemistry. 2021;217:111383.
doi:10.1016/j.jinorgbio.2021.111383 .

Drača, Dijana, Edeler, David, Saoud, Mohamad, Dojčinović, Biljana, Dunđerović, Duško, Đmura, Goran, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Kaluđerović, Goran N., "Antitumor potential of cisplatin loaded into SBA-15 mesoporous silica nanoparticles against B16F1 melanoma cells: in vitro and in vivo studies." in Journal of Inorganic Biochemistry, 217 (2021):111383,
https://doi.org/10.1016/j.jinorgbio.2021.111383 . .