TY  - JOUR
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Popov Aleksandrov, Aleksandra
AU  - Ukropina, Mirela
AU  - Cakić Milošević, Maja
AU  - Glamočlija, Jasmina
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0300483X20302730
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4033
AB  - Cadmium (Cd) is one of the most toxic environmental heavy metals to which the general population is exposed mainly via the oral route. Owing to its immunomodulatory potential, orally acquired Cd affects antimicrobial immune defense in several organs, including the lungs. While there are data concerning Cd and viral and bacterial pulmonary infections, effects on fungal infections are not studied yet. In the present study, the effect of the Cd (5 mg/L for 30 days, in drinking water, the average daily Cd intake 0.641 ± 0.089 mg/kg) on the immune response of rats to pulmonary A. fumigatus infection was examined. Data obtained showed that orally acquired cadmium does not affect the elimination of the fungus in immunocompetent rats owing to the preservation of some aspects of innate immune responses (lung leukocyte infiltration and NBT reduction) and an increase in other (increased numbers of mucus-producing goblet cells, MPO release). Cd does not affect an IFN-γ response in lung leukocytes during the infection (despite suppression of cytokine production in cells of lung-draining lymph nodes), while it stimulates IL-17 and suppresses IL-10 response to the fungus. As a result, the elimination of the fungus occurs in a milieu with the prevailing proinflammatory response in Cd-exposed animals that preserved fungal elimination from the lungs, though with more intense injury to the lung tissue. Therefore, the proinflammatory microenvironment in the lungs created by Cd that sustains inflammatory/immune response to the fungus to which humans are exposed for a lifetime, raises a concern of orally acquired Cd as a risk factor for the development of chronic low-grade pulmonary inflammation.
PB  - Elsevier Ireland Ltd
T2  - Toxicology
T2  - Toxicology
T1  - Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?
VL  - 447
DO  - 10.1016/j.tox.2020.152634
SP  - 152634
ER  - 

@article{
author = "Kulaš, Jelena and Tucović, Dina and Zeljković, Milica and Popović, Dušanka and Popov Aleksandrov, Aleksandra and Ukropina, Mirela and Cakić Milošević, Maja and Glamočlija, Jasmina and Kataranovski, Milena and Mirkov, Ivana",
year = "2021",
abstract = "Cadmium (Cd) is one of the most toxic environmental heavy metals to which the general population is exposed mainly via the oral route. Owing to its immunomodulatory potential, orally acquired Cd affects antimicrobial immune defense in several organs, including the lungs. While there are data concerning Cd and viral and bacterial pulmonary infections, effects on fungal infections are not studied yet. In the present study, the effect of the Cd (5 mg/L for 30 days, in drinking water, the average daily Cd intake 0.641 ± 0.089 mg/kg) on the immune response of rats to pulmonary A. fumigatus infection was examined. Data obtained showed that orally acquired cadmium does not affect the elimination of the fungus in immunocompetent rats owing to the preservation of some aspects of innate immune responses (lung leukocyte infiltration and NBT reduction) and an increase in other (increased numbers of mucus-producing goblet cells, MPO release). Cd does not affect an IFN-γ response in lung leukocytes during the infection (despite suppression of cytokine production in cells of lung-draining lymph nodes), while it stimulates IL-17 and suppresses IL-10 response to the fungus. As a result, the elimination of the fungus occurs in a milieu with the prevailing proinflammatory response in Cd-exposed animals that preserved fungal elimination from the lungs, though with more intense injury to the lung tissue. Therefore, the proinflammatory microenvironment in the lungs created by Cd that sustains inflammatory/immune response to the fungus to which humans are exposed for a lifetime, raises a concern of orally acquired Cd as a risk factor for the development of chronic low-grade pulmonary inflammation.",
publisher = "Elsevier Ireland Ltd",
journal = "Toxicology, Toxicology",
title = "Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?",
volume = "447",
doi = "10.1016/j.tox.2020.152634",
pages = "152634"
}

Kulaš, J., Tucović, D., Zeljković, M., Popović, D., Popov Aleksandrov, A., Ukropina, M., Cakić Milošević, M., Glamočlija, J., Kataranovski, M.,& Mirkov, I.. (2021). Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?. in Toxicology
Elsevier Ireland Ltd., 447, 152634.
https://doi.org/10.1016/j.tox.2020.152634

Kulaš J, Tucović D, Zeljković M, Popović D, Popov Aleksandrov A, Ukropina M, Cakić Milošević M, Glamočlija J, Kataranovski M, Mirkov I. Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?. in Toxicology. 2021;447:152634.
doi:10.1016/j.tox.2020.152634 .

Kulaš, Jelena, Tucović, Dina, Zeljković, Milica, Popović, Dušanka, Popov Aleksandrov, Aleksandra, Ukropina, Mirela, Cakić Milošević, Maja, Glamočlija, Jasmina, Kataranovski, Milena, Mirkov, Ivana, "Proinflammatory effects of environmental cadmium boost resistance to opportunistic pathogen Aspergillus fumigatus: Implications for sustained low-level pulmonary inflammation?" in Toxicology, 447 (2021):152634,
https://doi.org/10.1016/j.tox.2020.152634 . .

TY  - JOUR
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Popov Aleksandrov, Aleksandra
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
PY  - 2021
UR  - http://www.besjournal.com/en/article/doi/10.3967/bes2021.025
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4191
AB  - Objective To investigate involvement of the aryl hydrocarbon receptor (AhR) in the immunomodulatory effects of cadmium (Cd). Methods The effect of Cd on AhR activation ( CYP1A1 and CYP1B1 mRNA expression) was examined in lung leukocytes of Cd-exposed rats (5 and 50 mg/L, 30 d orally) and by in vitro leukocyte exposure. The involvement of AhR signaling in the effects of Cd on the interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) lung leukocyte response was investigated in vitro using the receptor antagonist CH-223191. Results Cd increased CYP1B1 ( in vivo and in vitro) and CYP1A1 ( in vitro) mRNA, indicating AhR involvement in the action of Cd. In response to Cd, lung leukocytes increased IL-6 and decreased TNF at the gene expression and protein levels, but decreased IL-1β production due to reduced NLRP3. The AhR antagonist CH-223191 abrogated the observed effects of Cd on the cytokine response. The absence of AhR reactivity and cytokine response to Cd of leukocytes from the lungs of a rat strain that is less sensitive to Cd toxicity coincided with a high AhR repressor mRNA level. Conclusion AhR signaling is involved in the lung leukocyte proinflammatory cytokine response to Cd. The relevance of the AhR to the cytokine response to Cd provides new insight into the mechanisms of Cd immunotoxicity.
PB  - Elsevier Ltd
T2  - Biomedical and Environmental Sciences
T1  - Aryl Hydrocarbon Receptor is Involved in the Proinflammatory Cytokine Response to Cadmium.
IS  - 3
VL  - 34
DO  - 10.3967/bes2021.025
SP  - 192
EP  - 202
ER  - 

@article{
author = "Kulaš, Jelena and Tucović, Dina and Zeljković, Milica and Popović, Dušanka and Popov Aleksandrov, Aleksandra and Kataranovski, Milena and Mirkov, Ivana",
year = "2021",
abstract = "Objective To investigate involvement of the aryl hydrocarbon receptor (AhR) in the immunomodulatory effects of cadmium (Cd). Methods The effect of Cd on AhR activation ( CYP1A1 and CYP1B1 mRNA expression) was examined in lung leukocytes of Cd-exposed rats (5 and 50 mg/L, 30 d orally) and by in vitro leukocyte exposure. The involvement of AhR signaling in the effects of Cd on the interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) lung leukocyte response was investigated in vitro using the receptor antagonist CH-223191. Results Cd increased CYP1B1 ( in vivo and in vitro) and CYP1A1 ( in vitro) mRNA, indicating AhR involvement in the action of Cd. In response to Cd, lung leukocytes increased IL-6 and decreased TNF at the gene expression and protein levels, but decreased IL-1β production due to reduced NLRP3. The AhR antagonist CH-223191 abrogated the observed effects of Cd on the cytokine response. The absence of AhR reactivity and cytokine response to Cd of leukocytes from the lungs of a rat strain that is less sensitive to Cd toxicity coincided with a high AhR repressor mRNA level. Conclusion AhR signaling is involved in the lung leukocyte proinflammatory cytokine response to Cd. The relevance of the AhR to the cytokine response to Cd provides new insight into the mechanisms of Cd immunotoxicity.",
publisher = "Elsevier Ltd",
journal = "Biomedical and Environmental Sciences",
title = "Aryl Hydrocarbon Receptor is Involved in the Proinflammatory Cytokine Response to Cadmium.",
number = "3",
volume = "34",
doi = "10.3967/bes2021.025",
pages = "192-202"
}

Kulaš, J., Tucović, D., Zeljković, M., Popović, D., Popov Aleksandrov, A., Kataranovski, M.,& Mirkov, I.. (2021). Aryl Hydrocarbon Receptor is Involved in the Proinflammatory Cytokine Response to Cadmium.. in Biomedical and Environmental Sciences
Elsevier Ltd., 34(3), 192-202.
https://doi.org/10.3967/bes2021.025

Kulaš J, Tucović D, Zeljković M, Popović D, Popov Aleksandrov A, Kataranovski M, Mirkov I. Aryl Hydrocarbon Receptor is Involved in the Proinflammatory Cytokine Response to Cadmium.. in Biomedical and Environmental Sciences. 2021;34(3):192-202.
doi:10.3967/bes2021.025 .

Kulaš, Jelena, Tucović, Dina, Zeljković, Milica, Popović, Dušanka, Popov Aleksandrov, Aleksandra, Kataranovski, Milena, Mirkov, Ivana, "Aryl Hydrocarbon Receptor is Involved in the Proinflammatory Cytokine Response to Cadmium." in Biomedical and Environmental Sciences, 34, no. 3 (2021):192-202,
https://doi.org/10.3967/bes2021.025 . .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Ninkov, Marina
AU  - Janković, Srđa
AU  - Kataranovski, Milena
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0165247821001644
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4664
AB  - Cadmium (Cd) represents a unique hazard because of the long biological half-life in humans (20-30 years). This metal accumulates in organs causing a continuum of responses, with organ disease/failure as extreme outcome. Some of the cellular and molecular alterations in target tissues can be related to immune-modulating potential of Cd. This metal may cause adverse responses in which components of the immune system function as both mediators and effectors of Cd tissue toxicity, which, in combination with Cd-induced alterations in homeostatic reparative activities may contribute to tissue dysfunction. In this work, current knowledge concerning inflammatory/autoimmune disease manifestations found to be related with cadmium exposure are summarized. Along with epidemiological evidence, animal and in vitro data are presented, with focus on cellular and molecular immune mechanisms potentially relevant for the disease susceptibility, disease promotion, or facilitating development of pre-existing pathologies.
PB  - Elsevier B.V.
T2  - Immunology Letters
T1  - Immunomodulation by heavy metals as a contributing factor to inflammatory diseases and autoimmune reactions: Cadmium as an example.
VL  - 240
DO  - 10.1016/j.imlet.2021.10.003
SP  - 106
EP  - 122
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Tucović, Dina and Kulaš, Jelena and Zeljković, Milica and Popović, Dušanka and Ninkov, Marina and Janković, Srđa and Kataranovski, Milena",
year = "2021",
abstract = "Cadmium (Cd) represents a unique hazard because of the long biological half-life in humans (20-30 years). This metal accumulates in organs causing a continuum of responses, with organ disease/failure as extreme outcome. Some of the cellular and molecular alterations in target tissues can be related to immune-modulating potential of Cd. This metal may cause adverse responses in which components of the immune system function as both mediators and effectors of Cd tissue toxicity, which, in combination with Cd-induced alterations in homeostatic reparative activities may contribute to tissue dysfunction. In this work, current knowledge concerning inflammatory/autoimmune disease manifestations found to be related with cadmium exposure are summarized. Along with epidemiological evidence, animal and in vitro data are presented, with focus on cellular and molecular immune mechanisms potentially relevant for the disease susceptibility, disease promotion, or facilitating development of pre-existing pathologies.",
publisher = "Elsevier B.V.",
journal = "Immunology Letters",
title = "Immunomodulation by heavy metals as a contributing factor to inflammatory diseases and autoimmune reactions: Cadmium as an example.",
volume = "240",
doi = "10.1016/j.imlet.2021.10.003",
pages = "106-122"
}

Popov Aleksandrov, A., Mirkov, I., Tucović, D., Kulaš, J., Zeljković, M., Popović, D., Ninkov, M., Janković, S.,& Kataranovski, M.. (2021). Immunomodulation by heavy metals as a contributing factor to inflammatory diseases and autoimmune reactions: Cadmium as an example.. in Immunology Letters
Elsevier B.V.., 240, 106-122.
https://doi.org/10.1016/j.imlet.2021.10.003

Popov Aleksandrov A, Mirkov I, Tucović D, Kulaš J, Zeljković M, Popović D, Ninkov M, Janković S, Kataranovski M. Immunomodulation by heavy metals as a contributing factor to inflammatory diseases and autoimmune reactions: Cadmium as an example.. in Immunology Letters. 2021;240:106-122.
doi:10.1016/j.imlet.2021.10.003 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Tucović, Dina, Kulaš, Jelena, Zeljković, Milica, Popović, Dušanka, Ninkov, Marina, Janković, Srđa, Kataranovski, Milena, "Immunomodulation by heavy metals as a contributing factor to inflammatory diseases and autoimmune reactions: Cadmium as an example." in Immunology Letters, 240 (2021):106-122,
https://doi.org/10.1016/j.imlet.2021.10.003 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Ninkov, Marina
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Kataranovski, Milena
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33508420
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4150
AB  - Cadmium (Cd) has been listed as one of the most toxic substances affecting numerous tissues/organs, including the immune system. Due to variations in studies examining Cd effects on the immune system (exposure regime, experimental systems, immune endpoint measured), data on Cd immunotoxicity in humans and experimental animals are inconsistent. However, it is clear that Cd can affect cells of the immune system and can modulate some immune responses. Due to the complex nature of the immune system and its activities which are determined by multiple interactions, the underlying mechanisms involved in the immunotoxicity of this metal are still vague. Here, the current knowledge regarding the interaction of Cd with cells of the immune system, which may affect immune responses as well as potential mechanisms of consequent biological effects of such activities, is reviewed. Tissue injury caused by Cd-induced effects on innate cell activities depicts components of the immune system as mediators/effectors of Cd tissue toxicity. Cd-induced immune alterations, which may compromise host defense against pathogenic microorganisms and homeostatic reparative activities, stress this metal as an important health hazard.
PB  - Elsevier BV
T2  - Food and Chemical Toxicology
T1  - Immunotoxicology of cadmium: Cells of the immune system as targets and effectors of cadmium toxicity.
VL  - 149
DO  - 10.1016/j.fct.2021.112026
SP  - 112026
ER  - 

@article{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Ninkov, Marina and Tucović, Dina and Kulaš, Jelena and Zeljković, Milica and Popović, Dušanka and Kataranovski, Milena",
year = "2021",
abstract = "Cadmium (Cd) has been listed as one of the most toxic substances affecting numerous tissues/organs, including the immune system. Due to variations in studies examining Cd effects on the immune system (exposure regime, experimental systems, immune endpoint measured), data on Cd immunotoxicity in humans and experimental animals are inconsistent. However, it is clear that Cd can affect cells of the immune system and can modulate some immune responses. Due to the complex nature of the immune system and its activities which are determined by multiple interactions, the underlying mechanisms involved in the immunotoxicity of this metal are still vague. Here, the current knowledge regarding the interaction of Cd with cells of the immune system, which may affect immune responses as well as potential mechanisms of consequent biological effects of such activities, is reviewed. Tissue injury caused by Cd-induced effects on innate cell activities depicts components of the immune system as mediators/effectors of Cd tissue toxicity. Cd-induced immune alterations, which may compromise host defense against pathogenic microorganisms and homeostatic reparative activities, stress this metal as an important health hazard.",
publisher = "Elsevier BV",
journal = "Food and Chemical Toxicology",
title = "Immunotoxicology of cadmium: Cells of the immune system as targets and effectors of cadmium toxicity.",
volume = "149",
doi = "10.1016/j.fct.2021.112026",
pages = "112026"
}

Mirkov, I., Popov Aleksandrov, A., Ninkov, M., Tucović, D., Kulaš, J., Zeljković, M., Popović, D.,& Kataranovski, M.. (2021). Immunotoxicology of cadmium: Cells of the immune system as targets and effectors of cadmium toxicity.. in Food and Chemical Toxicology
Elsevier BV., 149, 112026.
https://doi.org/10.1016/j.fct.2021.112026

Mirkov I, Popov Aleksandrov A, Ninkov M, Tucović D, Kulaš J, Zeljković M, Popović D, Kataranovski M. Immunotoxicology of cadmium: Cells of the immune system as targets and effectors of cadmium toxicity.. in Food and Chemical Toxicology. 2021;149:112026.
doi:10.1016/j.fct.2021.112026 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Ninkov, Marina, Tucović, Dina, Kulaš, Jelena, Zeljković, Milica, Popović, Dušanka, Kataranovski, Milena, "Immunotoxicology of cadmium: Cells of the immune system as targets and effectors of cadmium toxicity." in Food and Chemical Toxicology, 149 (2021):112026,
https://doi.org/10.1016/j.fct.2021.112026 . .

TY  - JOUR
AU  - Dragić, Milorad
AU  - Zeljković, Milica
AU  - Stevanović, Ivana
AU  - Ilić, Tihomir
AU  - Ilić, Nela
AU  - Nedeljković, Nadezda
AU  - Ninković, Milica
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32599126
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3768
AB  - Multiple sclerosis (MS) is a chronic neurodegenerative disease caused by inflammatory processes in the central nervous system (CNS). Decades of research led to discovery of several disease-modifying therapeutics strategies with moderate success. Experimental autoimmune encephalomyelitis (EAE) is currently the most commonly used experimental model for MS and for studying various therapeutic approaches. Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive neurostimulation technique with multiple beneficial effects on healthy as well as CNS with pathology. However, the molecular and cellular mechanisms of rTMS on acute EAE are scarce. Our study demonstrated beneficial effects of theta-burst stimulation (TBS), an experimental paradigm of rTMS, on disease course of acute EAE. TBS treatment attenuated reactive gliosis, restored myelin sheet and down-regulated expression of vimentin in EAE rats. These effects were reflected through reduced clinical parameters, shorter duration of illness and days spent in paralysis. Based on our research, rTMS deserves further considerations for its neuroprotective effect on EAE, and is an excellent candidate for further research and points that it could be used for more than for simple symptomatic therapy.
PB  - Elsevier BV
T2  - Brain Research Bulletin
T1  - Theta burst stimulation ameliorates symptoms of experimental autoimmune encephalomyelitis and attenuates reactive gliosis.
VL  - 162
DO  - 10.1016/j.brainresbull.2020.06.013
SP  - 208
EP  - 217
ER  - 

@article{
author = "Dragić, Milorad and Zeljković, Milica and Stevanović, Ivana and Ilić, Tihomir and Ilić, Nela and Nedeljković, Nadezda and Ninković, Milica",
year = "2020",
abstract = "Multiple sclerosis (MS) is a chronic neurodegenerative disease caused by inflammatory processes in the central nervous system (CNS). Decades of research led to discovery of several disease-modifying therapeutics strategies with moderate success. Experimental autoimmune encephalomyelitis (EAE) is currently the most commonly used experimental model for MS and for studying various therapeutic approaches. Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive neurostimulation technique with multiple beneficial effects on healthy as well as CNS with pathology. However, the molecular and cellular mechanisms of rTMS on acute EAE are scarce. Our study demonstrated beneficial effects of theta-burst stimulation (TBS), an experimental paradigm of rTMS, on disease course of acute EAE. TBS treatment attenuated reactive gliosis, restored myelin sheet and down-regulated expression of vimentin in EAE rats. These effects were reflected through reduced clinical parameters, shorter duration of illness and days spent in paralysis. Based on our research, rTMS deserves further considerations for its neuroprotective effect on EAE, and is an excellent candidate for further research and points that it could be used for more than for simple symptomatic therapy.",
publisher = "Elsevier BV",
journal = "Brain Research Bulletin",
title = "Theta burst stimulation ameliorates symptoms of experimental autoimmune encephalomyelitis and attenuates reactive gliosis.",
volume = "162",
doi = "10.1016/j.brainresbull.2020.06.013",
pages = "208-217"
}

Dragić, M., Zeljković, M., Stevanović, I., Ilić, T., Ilić, N., Nedeljković, N.,& Ninković, M.. (2020). Theta burst stimulation ameliorates symptoms of experimental autoimmune encephalomyelitis and attenuates reactive gliosis.. in Brain Research Bulletin
Elsevier BV., 162, 208-217.
https://doi.org/10.1016/j.brainresbull.2020.06.013

Dragić M, Zeljković M, Stevanović I, Ilić T, Ilić N, Nedeljković N, Ninković M. Theta burst stimulation ameliorates symptoms of experimental autoimmune encephalomyelitis and attenuates reactive gliosis.. in Brain Research Bulletin. 2020;162:208-217.
doi:10.1016/j.brainresbull.2020.06.013 .

Dragić, Milorad, Zeljković, Milica, Stevanović, Ivana, Ilić, Tihomir, Ilić, Nela, Nedeljković, Nadezda, Ninković, Milica, "Theta burst stimulation ameliorates symptoms of experimental autoimmune encephalomyelitis and attenuates reactive gliosis." in Brain Research Bulletin, 162 (2020):208-217,
https://doi.org/10.1016/j.brainresbull.2020.06.013 . .

TY  - CONF
AU  - Popović, Dušanka
AU  - Popov Aleksandrov, Aleksandra
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Zeljković, Milica
AU  - Terzić-Vidojević, Amarela
AU  - Lukić, Jovana
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Tolinački, Maja
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4793
AB  - The gut microbiome is very important for hosts’ proper essential functions, as microbiota influence both near and distant organs and disruption at any level of this complex relation can be underlying cause for many diseases. Both intestinal bacterial population and their metabolic products are involved in immune system development, activity and maintenance of immune homeostasis. Previous findings revealed differences in immune reactivity in Albino Oxford (AO) and Dark Agouti (DA) rat strains in various diseases models (such as experimental autoimmune encephalomyelitis, rheumatoid arthritis, contact hypersensitivity reaction, pulmonary aspergillosis etc.), and in response to xenobiotics such as cadmium, warfarin, etc.
The aim of present study was to investigate microbial composition of gut (duodenum, jejunum, coecum and colon) and lungs of AO and DA rats using DGGE method. Although similar number of bacterial species were noted in both tissue and lumen of duodenum, bacterial composition differ between these two strains (solely 76.2% and 44.4% species were common in both strains tissue and lumen, respectively), while greater variability was noted in DA rats. Similar results were noted in jejunum. In contrast to duodenum and jejunum, higher number of bacterial species were detected in coecum (content) and colon (tissue and content) of AO rats. Around 50% of detected bacteria were present in both gut segments of both strains. Analysis of DGGE bands obtained from lung tissue revealed similar number of bacteria in both strains, but solely 54.5% were common. Further investigations will be directed to identification of bacterial species and try to connect observed differences in microbial composition with different immune reactivity in AO and DA rats.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade
C3  - Immunology at the Confluence of Multidisciplinary Approaches : abstract book
T1  - Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4793
ER  - 

@conference{
author = "Popović, Dušanka and Popov Aleksandrov, Aleksandra and Tucović, Dina and Kulaš, Jelena and Zeljković, Milica and Terzić-Vidojević, Amarela and Lukić, Jovana and Golić, Nataša and Mirkov, Ivana and Tolinački, Maja",
year = "2019",
abstract = "The gut microbiome is very important for hosts’ proper essential functions, as microbiota influence both near and distant organs and disruption at any level of this complex relation can be underlying cause for many diseases. Both intestinal bacterial population and their metabolic products are involved in immune system development, activity and maintenance of immune homeostasis. Previous findings revealed differences in immune reactivity in Albino Oxford (AO) and Dark Agouti (DA) rat strains in various diseases models (such as experimental autoimmune encephalomyelitis, rheumatoid arthritis, contact hypersensitivity reaction, pulmonary aspergillosis etc.), and in response to xenobiotics such as cadmium, warfarin, etc.
The aim of present study was to investigate microbial composition of gut (duodenum, jejunum, coecum and colon) and lungs of AO and DA rats using DGGE method. Although similar number of bacterial species were noted in both tissue and lumen of duodenum, bacterial composition differ between these two strains (solely 76.2% and 44.4% species were common in both strains tissue and lumen, respectively), while greater variability was noted in DA rats. Similar results were noted in jejunum. In contrast to duodenum and jejunum, higher number of bacterial species were detected in coecum (content) and colon (tissue and content) of AO rats. Around 50% of detected bacteria were present in both gut segments of both strains. Analysis of DGGE bands obtained from lung tissue revealed similar number of bacteria in both strains, but solely 54.5% were common. Further investigations will be directed to identification of bacterial species and try to connect observed differences in microbial composition with different immune reactivity in AO and DA rats.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade",
journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book",
title = "Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly",
pages = "28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4793"
}

Popović, D., Popov Aleksandrov, A., Tucović, D., Kulaš, J., Zeljković, M., Terzić-Vidojević, A., Lukić, J., Golić, N., Mirkov, I.,& Tolinački, M.. (2019). Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book
Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade., 28.
https://hdl.handle.net/21.15107/rcub_ibiss_4793

Popović D, Popov Aleksandrov A, Tucović D, Kulaš J, Zeljković M, Terzić-Vidojević A, Lukić J, Golić N, Mirkov I, Tolinački M. Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book. 2019;:28.
https://hdl.handle.net/21.15107/rcub_ibiss_4793 .

Popović, Dušanka, Popov Aleksandrov, Aleksandra, Tucović, Dina, Kulaš, Jelena, Zeljković, Milica, Terzić-Vidojević, Amarela, Lukić, Jovana, Golić, Nataša, Mirkov, Ivana, Tolinački, Maja, "Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book (2019):28,
https://hdl.handle.net/21.15107/rcub_ibiss_4793 .

TY  - CONF
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Popov Aleksandrov, Aleksandra
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4794
AB  - Cadmium (Cd) is a heavy metal widely spread in the environment and significant water and food contaminant. This metal exerts toxic effects in various tissues thus representing great threat to human health, and our previous study showed that oral consumption of Cd (in water for 30 days) increased the metal deposition and exerted immunomodulatory effects in lung leukocytes. Although the most studied mechanisms of Cd toxicity include oxidative stress and inflammation, recent studies have indicated that this metal can activate aryl hydrocarbon receptor (AHR) and exert effects on AHR-regulated genes (i.e. CYPs). AHR represents a link between environmental toxicants and immune response as high receptor expression is noted in immune cells and barrier tissues, thus the aim of presented study was to investigate if activation of AhR by Cd is associated with metals’ immunomodulatory effects. Treatment of lung leukocytes with Cd in vitro (non-toxic doses) caused an increase in mRNA levels for AHR, CYP1B1 and CYP1A1, but co-treatment with metal and AHR antagonist CH223191 indicated that higher Cd doses (5 and 10 μM) can activate CYPs directly while a lower dose (1 μM) exerted effects on CYPs expression through activation of AHR. Low Cd dose induced increased production of IL-6 and decreased TNF and IL-1β by lung leukocytes, compared to controls. Gene expression data revealed unchanged mRNA for IL-6, decreased TNF, but increased IL-1β. Lower IL-1β protein level despite increased mRNA, was a consequence of decreased mRNA for NLRP3, a component of inflammasome that is involved in processing of pro-IL-1β in IL-1β. All noted effects were abolished in the presence of CH223191. Data obtained indicate that immunomodulatory effects of low Cd dose are mediated through AHR activation.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Immunology at the Confluence of Multidisciplinary Approaches : abstract book
T1  - Environmentally relevant exposure to cadmium and health risks: involvement of aryl hydrocarbon receptor
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4794
ER  - 

@conference{
author = "Kulaš, Jelena and Tucović, Dina and Zeljković, Milica and Popović, Dušanka and Popov Aleksandrov, Aleksandra and Kataranovski, Milena and Mirkov, Ivana",
year = "2019",
abstract = "Cadmium (Cd) is a heavy metal widely spread in the environment and significant water and food contaminant. This metal exerts toxic effects in various tissues thus representing great threat to human health, and our previous study showed that oral consumption of Cd (in water for 30 days) increased the metal deposition and exerted immunomodulatory effects in lung leukocytes. Although the most studied mechanisms of Cd toxicity include oxidative stress and inflammation, recent studies have indicated that this metal can activate aryl hydrocarbon receptor (AHR) and exert effects on AHR-regulated genes (i.e. CYPs). AHR represents a link between environmental toxicants and immune response as high receptor expression is noted in immune cells and barrier tissues, thus the aim of presented study was to investigate if activation of AhR by Cd is associated with metals’ immunomodulatory effects. Treatment of lung leukocytes with Cd in vitro (non-toxic doses) caused an increase in mRNA levels for AHR, CYP1B1 and CYP1A1, but co-treatment with metal and AHR antagonist CH223191 indicated that higher Cd doses (5 and 10 μM) can activate CYPs directly while a lower dose (1 μM) exerted effects on CYPs expression through activation of AHR. Low Cd dose induced increased production of IL-6 and decreased TNF and IL-1β by lung leukocytes, compared to controls. Gene expression data revealed unchanged mRNA for IL-6, decreased TNF, but increased IL-1β. Lower IL-1β protein level despite increased mRNA, was a consequence of decreased mRNA for NLRP3, a component of inflammasome that is involved in processing of pro-IL-1β in IL-1β. All noted effects were abolished in the presence of CH223191. Data obtained indicate that immunomodulatory effects of low Cd dose are mediated through AHR activation.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book",
title = "Environmentally relevant exposure to cadmium and health risks: involvement of aryl hydrocarbon receptor",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4794"
}

Kulaš, J., Tucović, D., Zeljković, M., Popović, D., Popov Aleksandrov, A., Kataranovski, M.,& Mirkov, I.. (2019). Environmentally relevant exposure to cadmium and health risks: involvement of aryl hydrocarbon receptor. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 80.
https://hdl.handle.net/21.15107/rcub_ibiss_4794

Kulaš J, Tucović D, Zeljković M, Popović D, Popov Aleksandrov A, Kataranovski M, Mirkov I. Environmentally relevant exposure to cadmium and health risks: involvement of aryl hydrocarbon receptor. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book. 2019;:80.
https://hdl.handle.net/21.15107/rcub_ibiss_4794 .

Kulaš, Jelena, Tucović, Dina, Zeljković, Milica, Popović, Dušanka, Popov Aleksandrov, Aleksandra, Kataranovski, Milena, Mirkov, Ivana, "Environmentally relevant exposure to cadmium and health risks: involvement of aryl hydrocarbon receptor" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book (2019):80,
https://hdl.handle.net/21.15107/rcub_ibiss_4794 .

TY  - CONF
AU  - Tucović, Dina
AU  - Mirkov, Ivana
AU  - Kulaš, Jelena
AU  - Zeljković, Milica
AU  - Popović, Dušanka
AU  - Zolotarevski, Lidija
AU  - Đurđić, Slađana
AU  - Mutić, Jelena
AU  - Kataranovski, Milena
AU  - Popov Aleksandrov, Aleksandra
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4795
AB  - Adverse effects of non-occupational exposure to cadmium (Cd) are increasingly
acknowledged. Using a rat model of oral Cd exposure in drinking water we have shown
that skin is a target for this metal. Due to contribution of individual variability to the
intensity of cadmium toxicity, dermatotoxicity of two environmentally relevant Cd
doses (5 and 50 ppm) was examined in individuals of two rat strains, Albino Oxford
(AO) and Dark Agouti (DA), which differ in response to chemicals. A dose-dependent
accumulation of Cd in the skin/epidermal cells was noted in both strains, and although
there were no strain differences in the Cd accumulation, the degree of skin response to
the metal differed. Signs of skin damage were evident in both strains, but response to
injury was more pronounced in DA. Individuals of DA rats responded by an increase
in the levels of antioxidant defense enzymes in the skin already at lower dose, in
contrast to AO (which reacted to higher dose solely), implying higher sensitivity of DA
strain to Cd-induced toxicity. Epidermal cells from both strains developed stress
response, however increased GSH, and higher metallothionein/MT-1 and MT-2
mRNA, Nrf2 protein, apoptosis, Ahr and Cyp genes in AO, depicting this strain`s
ability to better defend against Cd insult. Epidermal cells` IL-1β, TNF and IL-6
response was induced by Cd in DA, while pro-inflammatory cytokine production was
unchanged in AO (though increased following stimulation with S. epidermidis), with
increased IL-10 as a possible underlying mechanism. T cells from non-exposed rats
produce more IFN-γ and IL-17 in co-culture with epidermal cell from Cd–exposed DA
rats what strengthens the view that this strain is more prone to metal’s dermatotoxicity.
These data give a new insight into repercussion of genetic variability to toxicity of
cadmium acquired by the skin via gut, bearing relevance for variations in the link
between dietary cadmium and inflammation-based skin pathologies.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Immunology at the Confluence of Multidisciplinary Approaches : abstract book
T1  - Environmentally relevant exposure to cadmium and health risks: skin as target organ
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4795
ER  - 

@conference{
author = "Tucović, Dina and Mirkov, Ivana and Kulaš, Jelena and Zeljković, Milica and Popović, Dušanka and Zolotarevski, Lidija and Đurđić, Slađana and Mutić, Jelena and Kataranovski, Milena and Popov Aleksandrov, Aleksandra",
year = "2019",
abstract = "Adverse effects of non-occupational exposure to cadmium (Cd) are increasingly
acknowledged. Using a rat model of oral Cd exposure in drinking water we have shown
that skin is a target for this metal. Due to contribution of individual variability to the
intensity of cadmium toxicity, dermatotoxicity of two environmentally relevant Cd
doses (5 and 50 ppm) was examined in individuals of two rat strains, Albino Oxford
(AO) and Dark Agouti (DA), which differ in response to chemicals. A dose-dependent
accumulation of Cd in the skin/epidermal cells was noted in both strains, and although
there were no strain differences in the Cd accumulation, the degree of skin response to
the metal differed. Signs of skin damage were evident in both strains, but response to
injury was more pronounced in DA. Individuals of DA rats responded by an increase
in the levels of antioxidant defense enzymes in the skin already at lower dose, in
contrast to AO (which reacted to higher dose solely), implying higher sensitivity of DA
strain to Cd-induced toxicity. Epidermal cells from both strains developed stress
response, however increased GSH, and higher metallothionein/MT-1 and MT-2
mRNA, Nrf2 protein, apoptosis, Ahr and Cyp genes in AO, depicting this strain`s
ability to better defend against Cd insult. Epidermal cells` IL-1β, TNF and IL-6
response was induced by Cd in DA, while pro-inflammatory cytokine production was
unchanged in AO (though increased following stimulation with S. epidermidis), with
increased IL-10 as a possible underlying mechanism. T cells from non-exposed rats
produce more IFN-γ and IL-17 in co-culture with epidermal cell from Cd–exposed DA
rats what strengthens the view that this strain is more prone to metal’s dermatotoxicity.
These data give a new insight into repercussion of genetic variability to toxicity of
cadmium acquired by the skin via gut, bearing relevance for variations in the link
between dietary cadmium and inflammation-based skin pathologies.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book",
title = "Environmentally relevant exposure to cadmium and health risks: skin as target organ",
pages = "79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4795"
}

Tucović, D., Mirkov, I., Kulaš, J., Zeljković, M., Popović, D., Zolotarevski, L., Đurđić, S., Mutić, J., Kataranovski, M.,& Popov Aleksandrov, A.. (2019). Environmentally relevant exposure to cadmium and health risks: skin as target organ. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 79.
https://hdl.handle.net/21.15107/rcub_ibiss_4795

Tucović D, Mirkov I, Kulaš J, Zeljković M, Popović D, Zolotarevski L, Đurđić S, Mutić J, Kataranovski M, Popov Aleksandrov A. Environmentally relevant exposure to cadmium and health risks: skin as target organ. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book. 2019;:79.
https://hdl.handle.net/21.15107/rcub_ibiss_4795 .

Tucović, Dina, Mirkov, Ivana, Kulaš, Jelena, Zeljković, Milica, Popović, Dušanka, Zolotarevski, Lidija, Đurđić, Slađana, Mutić, Jelena, Kataranovski, Milena, Popov Aleksandrov, Aleksandra, "Environmentally relevant exposure to cadmium and health risks: skin as target organ" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book (2019):79,
https://hdl.handle.net/21.15107/rcub_ibiss_4795 .