TY  - JOUR
AU  - Ilić, Marija
AU  - Bugarski-Stanojević, Vanja
AU  - Jovanović, Bogdan
AU  - Stamenković, Gorana
AU  - Zorić, Katarina
AU  - Paunović, Momir
AU  - Crnobrnja-Isailović, Jelka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6705
AB  - Simple Summary: Rana temporaria is one of the most widespread Palearctic brown frogs, with two
main genetic clades in Europe, geographic spatial pattern of which is insufficiently known. We
analyzed samples from the understudied western and central Balkans to evaluate the haplotype
diversity of widely used 16SrRNA and MT-CYTB mitochondrial gene sequences and to recognize
the contour of a possible contact zone between the main clades. The results revealed a suture
zone between theWestern and Eastern Clades in the central part of the Balkan Peninsula. Overall,
haplotype diversity in the western and central Balkans sample is high. Harboring both main genetic
clades of R. temporaria qualifies the Balkan Peninsula as another important center of species’ genetic
diversity, as well as rich in unique haplotypes.
Abstract: Rana temporaria is one of the most widespread Palearctic brown frogs. We aimed to
clarify distribution pattern of two main genetic clades in the understudied Balkan peninsula by
using 16SrRNA and MT-CYTB sequences, already widely applied in analyses of populations from
other parts of Europe, while focusing on the broad area along the Morava river (central Balkans)
as a known gap in the species distribution. Additionally, we were interested in revealing the
extent of haplotype diversity within the main genetic clades in the Balkans, particularly around
the supposed suture zone. The results revealed a suture zone between the Western and Eastern
Clades in the central part of the Balkan Peninsula. This indicated the existence of a historical
barrier between the Balkan Mountain Belt and geographically close mountains surrounding the
Vlasina Plateau (Rhodope/Serbian–Macedonian Massif). The overall observed haplotype diversity in
populations of R. temporaria from the Balkan Peninsula seems high. Harboring both main genetic
clades of R. temporaria qualifies the Balkan Peninsula as another important center of species’ genetic
diversity, as well as rich in unique haplotypes. This points out the necessity of applying conservation
measures focused on the common European frog populations and habitats in this part of the species’
distribution area.
PB  - Basel: MDPI
T2  - Animals
T1  - Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria
IS  - 10
VL  - 14
DO  - 10.3390/ani14101430
SP  - 1430
ER  - 

@article{
author = "Ilić, Marija and Bugarski-Stanojević, Vanja and Jovanović, Bogdan and Stamenković, Gorana and Zorić, Katarina and Paunović, Momir and Crnobrnja-Isailović, Jelka",
year = "2024",
abstract = "Simple Summary: Rana temporaria is one of the most widespread Palearctic brown frogs, with two
main genetic clades in Europe, geographic spatial pattern of which is insufficiently known. We
analyzed samples from the understudied western and central Balkans to evaluate the haplotype
diversity of widely used 16SrRNA and MT-CYTB mitochondrial gene sequences and to recognize
the contour of a possible contact zone between the main clades. The results revealed a suture
zone between theWestern and Eastern Clades in the central part of the Balkan Peninsula. Overall,
haplotype diversity in the western and central Balkans sample is high. Harboring both main genetic
clades of R. temporaria qualifies the Balkan Peninsula as another important center of species’ genetic
diversity, as well as rich in unique haplotypes.
Abstract: Rana temporaria is one of the most widespread Palearctic brown frogs. We aimed to
clarify distribution pattern of two main genetic clades in the understudied Balkan peninsula by
using 16SrRNA and MT-CYTB sequences, already widely applied in analyses of populations from
other parts of Europe, while focusing on the broad area along the Morava river (central Balkans)
as a known gap in the species distribution. Additionally, we were interested in revealing the
extent of haplotype diversity within the main genetic clades in the Balkans, particularly around
the supposed suture zone. The results revealed a suture zone between the Western and Eastern
Clades in the central part of the Balkan Peninsula. This indicated the existence of a historical
barrier between the Balkan Mountain Belt and geographically close mountains surrounding the
Vlasina Plateau (Rhodope/Serbian–Macedonian Massif). The overall observed haplotype diversity in
populations of R. temporaria from the Balkan Peninsula seems high. Harboring both main genetic
clades of R. temporaria qualifies the Balkan Peninsula as another important center of species’ genetic
diversity, as well as rich in unique haplotypes. This points out the necessity of applying conservation
measures focused on the common European frog populations and habitats in this part of the species’
distribution area.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria",
number = "10",
volume = "14",
doi = "10.3390/ani14101430",
pages = "1430"
}

Ilić, M., Bugarski-Stanojević, V., Jovanović, B., Stamenković, G., Zorić, K., Paunović, M.,& Crnobrnja-Isailović, J.. (2024). Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria. in Animals
Basel: MDPI., 14(10), 1430.
https://doi.org/10.3390/ani14101430

Ilić M, Bugarski-Stanojević V, Jovanović B, Stamenković G, Zorić K, Paunović M, Crnobrnja-Isailović J. Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria. in Animals. 2024;14(10):1430.
doi:10.3390/ani14101430 .

Ilić, Marija, Bugarski-Stanojević, Vanja, Jovanović, Bogdan, Stamenković, Gorana, Zorić, Katarina, Paunović, Momir, Crnobrnja-Isailović, Jelka, "Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria" in Animals, 14, no. 10 (2024):1430,
https://doi.org/10.3390/ani14101430 . .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Đokić, Marko
AU  - Stamenković, Gorana
AU  - Barišić Klisarić, Nataša
AU  - Stojković, Oliver
AU  - Jojić, Vida
AU  - Savić, Ivo
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6587
AB  - Blind mole rats (genus Nannospalax) attract a great deal of attention because of their cancer resistance and longevity. Due to the high rate of chromosome rearrangements, 74 Nannospalax chromosomal forms have been discovered. The convergence of their external morphology complicates their taxonomy, and many cryptic species remain unrecognized. Thus, the European N. leucodon supersp. is listed in the IUCN Red List of Threatened Species with “Data Deficient” status. It is crucial for the conservation of biodiversity to clarify its taxonomy, to recognize each cryptic species, and assign to them the correct conservation status. Of the more than 20 chromosomal forms described within N. leucodon, five cryptic species occur in Serbia. The most threatened among them—N. l. syrmiensis, described and named 50 years ago in the regions of Srem, Belgrade and Mačva—has been declared extinct in the literature, which may have negative consequences for the conservation of wildlife genetic diversity. Through five years of fieldwork and comparison of 16SrRNA and MT-CYTB gene segments between old, archived teeth and recently collected material, we show that N. l. syrmiensis is not extinct. However, its habitat has been fragmented and reduced, owing primarily to anthropogenic impact. Therefore, detailed surveillance, population-structure studies, risk assessment, and appropriate conservation measures are needed.
PB  - Basel: MDPI
T2  - Animals
T1  - A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct
IS  - 5
VL  - 14
DO  - 10.3390/ani14050774
SP  - 774
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Đokić, Marko and Stamenković, Gorana and Barišić Klisarić, Nataša and Stojković, Oliver and Jojić, Vida and Savić, Ivo",
year = "2024",
abstract = "Blind mole rats (genus Nannospalax) attract a great deal of attention because of their cancer resistance and longevity. Due to the high rate of chromosome rearrangements, 74 Nannospalax chromosomal forms have been discovered. The convergence of their external morphology complicates their taxonomy, and many cryptic species remain unrecognized. Thus, the European N. leucodon supersp. is listed in the IUCN Red List of Threatened Species with “Data Deficient” status. It is crucial for the conservation of biodiversity to clarify its taxonomy, to recognize each cryptic species, and assign to them the correct conservation status. Of the more than 20 chromosomal forms described within N. leucodon, five cryptic species occur in Serbia. The most threatened among them—N. l. syrmiensis, described and named 50 years ago in the regions of Srem, Belgrade and Mačva—has been declared extinct in the literature, which may have negative consequences for the conservation of wildlife genetic diversity. Through five years of fieldwork and comparison of 16SrRNA and MT-CYTB gene segments between old, archived teeth and recently collected material, we show that N. l. syrmiensis is not extinct. However, its habitat has been fragmented and reduced, owing primarily to anthropogenic impact. Therefore, detailed surveillance, population-structure studies, risk assessment, and appropriate conservation measures are needed.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct",
number = "5",
volume = "14",
doi = "10.3390/ani14050774",
pages = "774"
}

Bugarski-Stanojević, V., Đokić, M., Stamenković, G., Barišić Klisarić, N., Stojković, O., Jojić, V.,& Savić, I.. (2024). A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct. in Animals
Basel: MDPI., 14(5), 774.
https://doi.org/10.3390/ani14050774

Bugarski-Stanojević V, Đokić M, Stamenković G, Barišić Klisarić N, Stojković O, Jojić V, Savić I. A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct. in Animals. 2024;14(5):774.
doi:10.3390/ani14050774 .

Bugarski-Stanojević, Vanja, Đokić, Marko, Stamenković, Gorana, Barišić Klisarić, Nataša, Stojković, Oliver, Jojić, Vida, Savić, Ivo, "A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct" in Animals, 14, no. 5 (2024):774,
https://doi.org/10.3390/ani14050774 . .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Jojić, Vida
AU  - Ćosić, Nada
AU  - Ćirović, Duško
AU  - Stojković, Oliver
AU  - Veličković, Jelena
AU  - Savić, Ivo
PY  - 2022
UR  - https://www.mdpi.com/2076-2615/12/9/1097
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9105853
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4964
AB  - We explored the cryptic speciation of the Nannospalax leucodon species complex, characterised by intense karyotype evolution and reduced phenotypic variability that has produced different lineages, out of which 25 are described as chromosomal forms (CFs), so many cryptic species remain unnoticed. Although some of them should be classified as threatened, they lack the official nomenclature necessary to be involved in conservation strategies. Reproductive isolation between seven CFs has previously been demonstrated. To investigate the amount and dynamics of genetic discrepancy that follows chromosomal changes, infer speciation levels, and obtain phylogenetic patterns, we analysed mitochondrial 16S rRNA and MT-CYTB nucleotide polymorphism among 17 CFs-the highest number studied so far. Phylogenetic trees delineated 11 CFs as separate clades. Evolutionary divergence values overlapped with acknowledged higher taxonomic categories, or sometimes exceeded them. The fact that CFs with higher 2n are evolutionary older corresponds to the fusion hypothesis of Nannospalax karyotype evolution. To participate in conservation strategies, N. leucodon classification should follow the biological species concept, and proposed cryptic species should be formally named, despite a lack of classical morphometric discrepancy. We draw attention towards the syrmiensis and montanosyrmiensis CFs, estimated to be endangered/critically endangered, and emphasise the need for detailed monitoring and population survey for other cryptic species.
PB  - Basel: MDPI
T2  - Animals
T1  - Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation.
IS  - 9
VL  - 12
DO  - 10.3390/ani12091097
SP  - 1097
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Jojić, Vida and Ćosić, Nada and Ćirović, Duško and Stojković, Oliver and Veličković, Jelena and Savić, Ivo",
year = "2022",
abstract = "We explored the cryptic speciation of the Nannospalax leucodon species complex, characterised by intense karyotype evolution and reduced phenotypic variability that has produced different lineages, out of which 25 are described as chromosomal forms (CFs), so many cryptic species remain unnoticed. Although some of them should be classified as threatened, they lack the official nomenclature necessary to be involved in conservation strategies. Reproductive isolation between seven CFs has previously been demonstrated. To investigate the amount and dynamics of genetic discrepancy that follows chromosomal changes, infer speciation levels, and obtain phylogenetic patterns, we analysed mitochondrial 16S rRNA and MT-CYTB nucleotide polymorphism among 17 CFs-the highest number studied so far. Phylogenetic trees delineated 11 CFs as separate clades. Evolutionary divergence values overlapped with acknowledged higher taxonomic categories, or sometimes exceeded them. The fact that CFs with higher 2n are evolutionary older corresponds to the fusion hypothesis of Nannospalax karyotype evolution. To participate in conservation strategies, N. leucodon classification should follow the biological species concept, and proposed cryptic species should be formally named, despite a lack of classical morphometric discrepancy. We draw attention towards the syrmiensis and montanosyrmiensis CFs, estimated to be endangered/critically endangered, and emphasise the need for detailed monitoring and population survey for other cryptic species.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation.",
number = "9",
volume = "12",
doi = "10.3390/ani12091097",
pages = "1097"
}

Bugarski-Stanojević, V., Stamenković, G., Jojić, V., Ćosić, N., Ćirović, D., Stojković, O., Veličković, J.,& Savić, I.. (2022). Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation.. in Animals
Basel: MDPI., 12(9), 1097.
https://doi.org/10.3390/ani12091097

Bugarski-Stanojević V, Stamenković G, Jojić V, Ćosić N, Ćirović D, Stojković O, Veličković J, Savić I. Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation.. in Animals. 2022;12(9):1097.
doi:10.3390/ani12091097 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Jojić, Vida, Ćosić, Nada, Ćirović, Duško, Stojković, Oliver, Veličković, Jelena, Savić, Ivo, "Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation." in Animals, 12, no. 9 (2022):1097,
https://doi.org/10.3390/ani12091097 . .

TY  - CONF
AU  - Ilić, Marija
AU  - Jovanović, Bogdan
AU  - Stamenković, Gorana
AU  - Bugarski-Stanojević, Vanja
AU  - Paunović, Momir
AU  - Crnobrnja-Isailović, Jelka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5374
AB  - The common frog (Rana temporaria Linnaeus, 1758) is one of the most
widespread and abundant amphibians in Europe, except in the southern parts of the
continent, where distribution is apparently fragmented. It has the greatest genetic
variability of all western Palearctic brown frogs and it is extremely variable in
morphology and ecological preferences, also. The Republic of Serbia is a country of
particular interest in terms of amphibian conservation because of the genetically and
morphologically diverse populations of several amphibian species.
We analysed nucleotide variability of mitochondrial DNA sequences of partial
MT-CYTB gene (461 bp) of 27 specimens of R. temporaria species. Samples (eggs and
tail tips of adult individuals) were collected in two periods, 1986-2007 and 2013-2017,
in 14 localities in Serbia. The analyses revealed nine different haplotypes separated
into two main clusters: north-eastern and eastern (localities Bela Crkva, Grza, Bigar,
Đerdap, Stara planina) and central, west, south-western, and south-eastern Serbia
(localities Jagodnja, Lučani, Zlatibor, Goč, Kopaonik, Prokletije, Šar planina, Oštrozub,
and Vlasina). Lučani and Grza populations had two different haplotypes each.
Our results confirmed previous findings that the common frog shows
phylogeographic substructuring. The existence of two genetically diverged population
groups on the territory of Serbia suggests that these populations should be adequately
protected in terms of the conservation of their breeding places and suitable terrestrial
habitats. In Serbia, R. temporaria habitats are currently not only fragmented but also
under intensive anthropogenic pressure, due to intensive urbanization, particularly in
the mountain areas and the promotion of non-sustainable tourism, which makes them
highly vulnerable. Therefore, there is a need for more precise data about species’
phylogeny and distribution, both at the national and regional level, to define
conservation priorities in the near future
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Phylogeographic substructuring of the common frog (Rana temporaria) in Serbia
SP  - 145
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5374
ER  - 

@conference{
author = "Ilić, Marija and Jovanović, Bogdan and Stamenković, Gorana and Bugarski-Stanojević, Vanja and Paunović, Momir and Crnobrnja-Isailović, Jelka",
year = "2022",
abstract = "The common frog (Rana temporaria Linnaeus, 1758) is one of the most
widespread and abundant amphibians in Europe, except in the southern parts of the
continent, where distribution is apparently fragmented. It has the greatest genetic
variability of all western Palearctic brown frogs and it is extremely variable in
morphology and ecological preferences, also. The Republic of Serbia is a country of
particular interest in terms of amphibian conservation because of the genetically and
morphologically diverse populations of several amphibian species.
We analysed nucleotide variability of mitochondrial DNA sequences of partial
MT-CYTB gene (461 bp) of 27 specimens of R. temporaria species. Samples (eggs and
tail tips of adult individuals) were collected in two periods, 1986-2007 and 2013-2017,
in 14 localities in Serbia. The analyses revealed nine different haplotypes separated
into two main clusters: north-eastern and eastern (localities Bela Crkva, Grza, Bigar,
Đerdap, Stara planina) and central, west, south-western, and south-eastern Serbia
(localities Jagodnja, Lučani, Zlatibor, Goč, Kopaonik, Prokletije, Šar planina, Oštrozub,
and Vlasina). Lučani and Grza populations had two different haplotypes each.
Our results confirmed previous findings that the common frog shows
phylogeographic substructuring. The existence of two genetically diverged population
groups on the territory of Serbia suggests that these populations should be adequately
protected in terms of the conservation of their breeding places and suitable terrestrial
habitats. In Serbia, R. temporaria habitats are currently not only fragmented but also
under intensive anthropogenic pressure, due to intensive urbanization, particularly in
the mountain areas and the promotion of non-sustainable tourism, which makes them
highly vulnerable. Therefore, there is a need for more precise data about species’
phylogeny and distribution, both at the national and regional level, to define
conservation priorities in the near future",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Phylogeographic substructuring of the common frog (Rana temporaria) in Serbia",
pages = "145",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5374"
}

Ilić, M., Jovanović, B., Stamenković, G., Bugarski-Stanojević, V., Paunović, M.,& Crnobrnja-Isailović, J.. (2022). Phylogeographic substructuring of the common frog (Rana temporaria) in Serbia. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 145.
https://hdl.handle.net/21.15107/rcub_ibiss_5374

Ilić M, Jovanović B, Stamenković G, Bugarski-Stanojević V, Paunović M, Crnobrnja-Isailović J. Phylogeographic substructuring of the common frog (Rana temporaria) in Serbia. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:145.
https://hdl.handle.net/21.15107/rcub_ibiss_5374 .

Ilić, Marija, Jovanović, Bogdan, Stamenković, Gorana, Bugarski-Stanojević, Vanja, Paunović, Momir, Crnobrnja-Isailović, Jelka, "Phylogeographic substructuring of the common frog (Rana temporaria) in Serbia" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):145,
https://hdl.handle.net/21.15107/rcub_ibiss_5374 .

TY  - CONF
AU  - Ilić, Marija
AU  - Jovanović, Bogdan
AU  - Stamenković, Gorana
AU  - Bugarski-Stanojević, Vanja
AU  - Zorić, Katarina
AU  - Paunović, Momir
AU  - Crnobrnja-Isailović, Jelka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5373
AB  - Жаба травњача (Rana temporaria Linnaeus, 1758) у Србији насељава претежно планинске и високопланинске пределе. Међутим, због фрагментисаног ареала и нарушених станишта сматра се ретком врстом. Према националној Црвеној Књизи водоземаца, а на основу IUCN категоризације, има статус скоро угрожене врсте (NT – „Near Threatened“), а према Правилнику о проглашењу и заштити строго заштићених и заштићених дивљих биљака, животиња и гљива на територији Србије је строго заштићена врста.
У овом истраживању анализирани су узорци ткива (јаја, врх репа пуноглавца, врх прста адулта) од укупно 28 јединки травњаче са 14 локалитета на територији Србије сакупљених у периоду 1986-2007. и 2013-17. Парцијалне секвенце 16S rRNK гена митохондријалне ДНК (395 бп) показале су постојање шест хаплотипова, сврстаних у две групе које су и географски јасно раздвојене. Првој припадају јединке са локалитета Бела Црква, Бигар, Грза, Ђердап и Стара планина, а другој оне са подручја централне, западне, југозападне и југоисточне Србије (Лучани, Копаоник, Гоч, Јагодња, Проклетије, Златибор, Власина, Оштрозуб, Шар планина). На локалитету Лучани присутна су два различита хаплотипа.
Анализа гена 16S rRNK се показала као адекватна метода за испитивање генетичке и филогеографске структурираности врсте R. temporaria која се налази под интензивним антропогеним притиском. Због угрожавања генофонда, пре свега губитком повољних станшта и променом климе, постоји опасност драстичног смањења дистрибуције ове врсте у Србији, те је неопходно донешење мера у циљу њене заштите и конзервације.
AB  - Žaba travnjača (Rana temporaria Linnaeus, 1758) u Srbiji naseljava pretežno planinske i visokoplaninske predele. Međutim, zbog fragmentisanog areala i narušenih staništa smatra se retkom vrstom. Prema nacionalnoj Crvenoj Knjizi vodozemaca, a na osnovu IUCN kategorizacije, ima status skoro ugrožene vrste (NT – „Near Threatened“), a prema Pravilniku o proglašenju i zaštiti strogo zaštićenih i zaštićenih divljih biljaka, životinja i gljiva na teritoriji Srbije je strogo zaštićena vrsta. U ovom istraživanju analizirani su uzorci tkiva (jaja, vrh repa punoglavca, vrh prsta adulta) od ukupno 28 jedinki travnjače sa 14 lokaliteta na teritoriji Srbije sakupljenih u periodu 1986-2007. i 2013-17. Parcijalne sekvence 16S rRNK gena mitohondrijalne DNK (395 bp) pokazale su postojanje šest haplotipova, svrstanih u dve grupe koje su i geografski jasno razdvojene. Prvoj pripadaju jedinke sa lokaliteta Bela Crkva, Bigar, Grza, Đerdap i Stara planina, a drugoj one sa područja centralne, zapadne, jugozapadne i jugoistočne Srbije (Lučani, Kopaonik, Goč, Jagodnja, Prokletije, Zlatibor, Vlasina, Oštrozub, Šar planina). Na lokalitetu Lučani prisutna su dva različita haplotipa. Analiza gena 16S rRNK se pokazala kao adekvatna metoda za ispitivanje genetičke i filogeografske strukturiranosti vrste R. temporaria koja se nalazi pod intenzivnim antropogenim pritiskom. Zbog ugrožavanja genofonda, pre svega gubitkom povoljnih stanšta i promenom klime, postoji opasnost drastičnog smanjenja distribucije ove vrste u Srbiji, te je neophodno donešenje mera u cilju njene zaštite i konzervacije.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Polimorfizam 16S rRNK gena vrste Rana temporaria na teritoriji Srbije
T1  - Полиморфизам 16S rRNK гена врсте Rana temporaria на територији Србије
SP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5373
ER  - 

@conference{
author = "Ilić, Marija and Jovanović, Bogdan and Stamenković, Gorana and Bugarski-Stanojević, Vanja and Zorić, Katarina and Paunović, Momir and Crnobrnja-Isailović, Jelka",
year = "2022",
abstract = "Жаба травњача (Rana temporaria Linnaeus, 1758) у Србији насељава претежно планинске и високопланинске пределе. Међутим, због фрагментисаног ареала и нарушених станишта сматра се ретком врстом. Према националној Црвеној Књизи водоземаца, а на основу IUCN категоризације, има статус скоро угрожене врсте (NT – „Near Threatened“), а према Правилнику о проглашењу и заштити строго заштићених и заштићених дивљих биљака, животиња и гљива на територији Србије је строго заштићена врста.
У овом истраживању анализирани су узорци ткива (јаја, врх репа пуноглавца, врх прста адулта) од укупно 28 јединки травњаче са 14 локалитета на територији Србије сакупљених у периоду 1986-2007. и 2013-17. Парцијалне секвенце 16S rRNK гена митохондријалне ДНК (395 бп) показале су постојање шест хаплотипова, сврстаних у две групе које су и географски јасно раздвојене. Првој припадају јединке са локалитета Бела Црква, Бигар, Грза, Ђердап и Стара планина, а другој оне са подручја централне, западне, југозападне и југоисточне Србије (Лучани, Копаоник, Гоч, Јагодња, Проклетије, Златибор, Власина, Оштрозуб, Шар планина). На локалитету Лучани присутна су два различита хаплотипа.
Анализа гена 16S rRNK се показала као адекватна метода за испитивање генетичке и филогеографске структурираности врсте R. temporaria која се налази под интензивним антропогеним притиском. Због угрожавања генофонда, пре свега губитком повољних станшта и променом климе, постоји опасност драстичног смањења дистрибуције ове врсте у Србији, те је неопходно донешење мера у циљу њене заштите и конзервације., Žaba travnjača (Rana temporaria Linnaeus, 1758) u Srbiji naseljava pretežno planinske i visokoplaninske predele. Međutim, zbog fragmentisanog areala i narušenih staništa smatra se retkom vrstom. Prema nacionalnoj Crvenoj Knjizi vodozemaca, a na osnovu IUCN kategorizacije, ima status skoro ugrožene vrste (NT – „Near Threatened“), a prema Pravilniku o proglašenju i zaštiti strogo zaštićenih i zaštićenih divljih biljaka, životinja i gljiva na teritoriji Srbije je strogo zaštićena vrsta. U ovom istraživanju analizirani su uzorci tkiva (jaja, vrh repa punoglavca, vrh prsta adulta) od ukupno 28 jedinki travnjače sa 14 lokaliteta na teritoriji Srbije sakupljenih u periodu 1986-2007. i 2013-17. Parcijalne sekvence 16S rRNK gena mitohondrijalne DNK (395 bp) pokazale su postojanje šest haplotipova, svrstanih u dve grupe koje su i geografski jasno razdvojene. Prvoj pripadaju jedinke sa lokaliteta Bela Crkva, Bigar, Grza, Đerdap i Stara planina, a drugoj one sa područja centralne, zapadne, jugozapadne i jugoistočne Srbije (Lučani, Kopaonik, Goč, Jagodnja, Prokletije, Zlatibor, Vlasina, Oštrozub, Šar planina). Na lokalitetu Lučani prisutna su dva različita haplotipa. Analiza gena 16S rRNK se pokazala kao adekvatna metoda za ispitivanje genetičke i filogeografske strukturiranosti vrste R. temporaria koja se nalazi pod intenzivnim antropogenim pritiskom. Zbog ugrožavanja genofonda, pre svega gubitkom povoljnih stanšta i promenom klime, postoji opasnost drastičnog smanjenja distribucije ove vrste u Srbiji, te je neophodno donešenje mera u cilju njene zaštite i konzervacije.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Polimorfizam 16S rRNK gena vrste Rana temporaria na teritoriji Srbije, Полиморфизам 16S rRNK гена врсте Rana temporaria на територији Србије",
pages = "104",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5373"
}

Ilić, M., Jovanović, B., Stamenković, G., Bugarski-Stanojević, V., Zorić, K., Paunović, M.,& Crnobrnja-Isailović, J.. (2022). Polimorfizam 16S rRNK gena vrste Rana temporaria na teritoriji Srbije. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 104.
https://hdl.handle.net/21.15107/rcub_ibiss_5373

Ilić M, Jovanović B, Stamenković G, Bugarski-Stanojević V, Zorić K, Paunović M, Crnobrnja-Isailović J. Polimorfizam 16S rRNK gena vrste Rana temporaria na teritoriji Srbije. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:104.
https://hdl.handle.net/21.15107/rcub_ibiss_5373 .

Ilić, Marija, Jovanović, Bogdan, Stamenković, Gorana, Bugarski-Stanojević, Vanja, Zorić, Katarina, Paunović, Momir, Crnobrnja-Isailović, Jelka, "Polimorfizam 16S rRNK gena vrste Rana temporaria na teritoriji Srbije" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):104,
https://hdl.handle.net/21.15107/rcub_ibiss_5373 .

TY  - CONF
AU  - Đokić, Marko
AU  - Stamenković, Gorana
AU  - Jojić, Vida
AU  - Ćosić, Nada
AU  - Ćirović, Duško
AU  - Savić, Ivo
AU  - Bugarski-Stanojević, Vanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5312
AB  - Vrste slepog kučeta iz roda Nannospalax se, pored brojnih adaptacija na podzemni način života, karakterišu i izraženom varijabilnošću kariotipa. Do sada su opisane čak 74 različite hromozomske forme (HF), ali su zvanično priznate samo tri vrste.1 Svaka je zapravo kompleks vrsta sa statusom „nedovoljno podataka“ na IUCN Red List of Threatened Species. Evropske vrste ovog roda sačinjavaju kompleks N. leucodon supersp.1 Među njima je više od 20 različitih HF, od kojih je pet (hungaricus, serbicus, montanoserbicus, syrmiensis, montanosyrmiensis) prisutno u Srbiji, a poslednje dve se vode kao ugrožene.2 Iako su međusobno reproduktivno izolovane i filogenetski udaljene, zbog nedostatka morfoloških razlika predstavljaju kriptične vrste. Sa konzervacionog aspekta je veoma važno vršiti monitoring njihovih populacija i utvrditi areale rasprostanjenja. Stoga je neophodno razviti pouzdanu i neinvazivnu metodu za njihovu efikasnu determinaciju. Inter Simple Sequence Repeat ˗ Polymerase Chain Reaction (ISSR-PCR) tehnika, koja produkuje ISSR markere – anonimne sekvence DNK ograničene invertovanim tandemskim ponovcima, se pokazala kao korisna u identifikaciji morfološki sličnih vrsta kod nekoliko sisarskih taksona.3 Od 11 različitih ISSR prajmera testiranih na prisustvo fragmenata DNK specifičnih za vrstu, na ukupnom uzorku od 25 jedinki, tri prajmera (CAA)5GC; (CAG)4AC i (GA)8AC su dala informativne profile DNK, obezbeđujući pouzdano i jasno prepoznavanje svih pet analiziranih kriptičnih vrsta.
AB  - Врсте слепог кучета из рода Nannospalax се, поред бројних адаптација на подземни начин живота, карактеришу и израженом варијабилношћу кариотипа. До сада су описане чак 74 различите хромозомске форме (ХФ), али су званично признате само три врсте.1 Свака је заправо комплекс врста са статусом „недовољно података“ на IUCN Red List of Threatened Species. Европске врсте овог рода сачињавају комплекс N. leucodon supersp.1 Међу њима је више од 20 различитих ХФ, од којих је пет (hungaricus, serbicus, montanoserbicus, syrmiensis, montanosyrmiensis) присутно у Србији, а последње две се воде као угрожене.2 Иако су међусобно репродуктивно изоловане и филогенетски удаљене, због недостатка морфолошких разлика представљају криптичне врсте. Са конзервационог аспекта је веома важно вршити мониторинг њихових популација и утврдити ареале распростањења. Стога је неопходно развити поуздану и неинвазивну методу за њихову ефикасну детерминацију. Inter Simple Sequence Repeat ˗ Polymerase Chain Reaction (ISSR-PCR) техника, која продукује ISSR маркере – анонимне секвенце ДНК ограничене инвертованим тандемским поновцима, се показала као корисна у идентификацији морфолошки сличних врста код неколико сисарских таксона.3 Од 11 различитих ISSR прајмера тестираних на присуство фрагмената ДНК специфичних за врсту, на укупном узорку од 25 јединки, три прајмера (CAA)5GC; (CAG)4AC и (GA)8AC су дала информативне профиле ДНК, обезбеђујући поуздано и јасно препознавање свих пет анализираних криптичних врста.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia)
T1  - ISSR-PCR као једноставна дијагностичка метода за разликовање криптичних врста Nannospalax leucodon (Spalacinae, Rodentia)
SP  - 106
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5312
ER  - 

@conference{
author = "Đokić, Marko and Stamenković, Gorana and Jojić, Vida and Ćosić, Nada and Ćirović, Duško and Savić, Ivo and Bugarski-Stanojević, Vanja",
year = "2022",
abstract = "Vrste slepog kučeta iz roda Nannospalax se, pored brojnih adaptacija na podzemni način života, karakterišu i izraženom varijabilnošću kariotipa. Do sada su opisane čak 74 različite hromozomske forme (HF), ali su zvanično priznate samo tri vrste.1 Svaka je zapravo kompleks vrsta sa statusom „nedovoljno podataka“ na IUCN Red List of Threatened Species. Evropske vrste ovog roda sačinjavaju kompleks N. leucodon supersp.1 Među njima je više od 20 različitih HF, od kojih je pet (hungaricus, serbicus, montanoserbicus, syrmiensis, montanosyrmiensis) prisutno u Srbiji, a poslednje dve se vode kao ugrožene.2 Iako su međusobno reproduktivno izolovane i filogenetski udaljene, zbog nedostatka morfoloških razlika predstavljaju kriptične vrste. Sa konzervacionog aspekta je veoma važno vršiti monitoring njihovih populacija i utvrditi areale rasprostanjenja. Stoga je neophodno razviti pouzdanu i neinvazivnu metodu za njihovu efikasnu determinaciju. Inter Simple Sequence Repeat ˗ Polymerase Chain Reaction (ISSR-PCR) tehnika, koja produkuje ISSR markere – anonimne sekvence DNK ograničene invertovanim tandemskim ponovcima, se pokazala kao korisna u identifikaciji morfološki sličnih vrsta kod nekoliko sisarskih taksona.3 Od 11 različitih ISSR prajmera testiranih na prisustvo fragmenata DNK specifičnih za vrstu, na ukupnom uzorku od 25 jedinki, tri prajmera (CAA)5GC; (CAG)4AC i (GA)8AC su dala informativne profile DNK, obezbeđujući pouzdano i jasno prepoznavanje svih pet analiziranih kriptičnih vrsta., Врсте слепог кучета из рода Nannospalax се, поред бројних адаптација на подземни начин живота, карактеришу и израженом варијабилношћу кариотипа. До сада су описане чак 74 различите хромозомске форме (ХФ), али су званично признате само три врсте.1 Свака је заправо комплекс врста са статусом „недовољно података“ на IUCN Red List of Threatened Species. Европске врсте овог рода сачињавају комплекс N. leucodon supersp.1 Међу њима је више од 20 различитих ХФ, од којих је пет (hungaricus, serbicus, montanoserbicus, syrmiensis, montanosyrmiensis) присутно у Србији, а последње две се воде као угрожене.2 Иако су међусобно репродуктивно изоловане и филогенетски удаљене, због недостатка морфолошких разлика представљају криптичне врсте. Са конзервационог аспекта је веома важно вршити мониторинг њихових популација и утврдити ареале распростањења. Стога је неопходно развити поуздану и неинвазивну методу за њихову ефикасну детерминацију. Inter Simple Sequence Repeat ˗ Polymerase Chain Reaction (ISSR-PCR) техника, која продукује ISSR маркере – анонимне секвенце ДНК ограничене инвертованим тандемским поновцима, се показала као корисна у идентификацији морфолошки сличних врста код неколико сисарских таксона.3 Од 11 различитих ISSR прајмера тестираних на присуство фрагмената ДНК специфичних за врсту, на укупном узорку од 25 јединки, три прајмера (CAA)5GC; (CAG)4AC и (GA)8AC су дала информативне профиле ДНК, обезбеђујући поуздано и јасно препознавање свих пет анализираних криптичних врста.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia), ISSR-PCR као једноставна дијагностичка метода за разликовање криптичних врста Nannospalax leucodon (Spalacinae, Rodentia)",
pages = "106",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5312"
}

Đokić, M., Stamenković, G., Jojić, V., Ćosić, N., Ćirović, D., Savić, I.,& Bugarski-Stanojević, V.. (2022). ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 106.
https://hdl.handle.net/21.15107/rcub_ibiss_5312

Đokić M, Stamenković G, Jojić V, Ćosić N, Ćirović D, Savić I, Bugarski-Stanojević V. ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:106.
https://hdl.handle.net/21.15107/rcub_ibiss_5312 .

Đokić, Marko, Stamenković, Gorana, Jojić, Vida, Ćosić, Nada, Ćirović, Duško, Savić, Ivo, Bugarski-Stanojević, Vanja, "ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):106,
https://hdl.handle.net/21.15107/rcub_ibiss_5312 .

TY  - JOUR
AU  - Todorović, Lidija
AU  - Stamenković, Gorana
AU  - Vučetić-Tadić, Biljana
AU  - Umezawa, Kazuo
AU  - Božović, Ana
AU  - Yamashita, Shunichi
AU  - Stanojević, Boban
PY  - 2021
UR  - https://doi.org/10.2298/ABS201010055T
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6068
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4199
AB  - The use of targeted inhibitors has shown promise as an effective approach in cancer therapy. However, targeted therapies based only on one drug, such as 17-allylamino-17-demethoxygeldanamycin (17-AAG), have limited success, partly because cancer cells engage alternate pathways for survival and proliferation. In the present study, we evaluated whether dehydroxymethylepoxyquinomicin (DHMEQ), a nuclear factor ?B (NF-?B) inhibitor, can enhance the antitumor activities of 17-AAG, a 90 kDa heat shock protein (Hsp90) inhibitor, in the anaplastic thyroid cancer cell line KTC2. We examined the effect of combined drug treatment vs single drug treatment on cell survival. Isobologram analysis was performed to distinguish the additive vs synergistic effects of the drug combination. Western blotting was performed to investigate apoptosis markers: caspase 3, poly(ADP-ribose) polymerase-one (PARP-1), B-cell lymphoma-extra large (Bcl-XL), X-linked inhibitor of apoptosis (XIAP) and cellular inhibitor of apoptosis 2 (cIAP-2). Compared to monotherapy, the combined treatment enhanced growth-inhibitory effects in a synergistic manner and strongly potentiated apoptosis. These results demonstrate the first in vitro evidence that a combination of Hsp90 and NF-?B inhibitors is a more effective modality for inhibiting cell proliferation and survival in anaplastic thyroid carcinoma cells than either agent alone, warranting further investigations.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2
IS  - 1
VL  - 73
DO  - 10.2298/abs201010055t
SP  - 31
EP  - 38
ER  - 

@article{
author = "Todorović, Lidija and Stamenković, Gorana and Vučetić-Tadić, Biljana and Umezawa, Kazuo and Božović, Ana and Yamashita, Shunichi and Stanojević, Boban",
year = "2021",
abstract = "The use of targeted inhibitors has shown promise as an effective approach in cancer therapy. However, targeted therapies based only on one drug, such as 17-allylamino-17-demethoxygeldanamycin (17-AAG), have limited success, partly because cancer cells engage alternate pathways for survival and proliferation. In the present study, we evaluated whether dehydroxymethylepoxyquinomicin (DHMEQ), a nuclear factor ?B (NF-?B) inhibitor, can enhance the antitumor activities of 17-AAG, a 90 kDa heat shock protein (Hsp90) inhibitor, in the anaplastic thyroid cancer cell line KTC2. We examined the effect of combined drug treatment vs single drug treatment on cell survival. Isobologram analysis was performed to distinguish the additive vs synergistic effects of the drug combination. Western blotting was performed to investigate apoptosis markers: caspase 3, poly(ADP-ribose) polymerase-one (PARP-1), B-cell lymphoma-extra large (Bcl-XL), X-linked inhibitor of apoptosis (XIAP) and cellular inhibitor of apoptosis 2 (cIAP-2). Compared to monotherapy, the combined treatment enhanced growth-inhibitory effects in a synergistic manner and strongly potentiated apoptosis. These results demonstrate the first in vitro evidence that a combination of Hsp90 and NF-?B inhibitors is a more effective modality for inhibiting cell proliferation and survival in anaplastic thyroid carcinoma cells than either agent alone, warranting further investigations.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2",
number = "1",
volume = "73",
doi = "10.2298/abs201010055t",
pages = "31-38"
}

Todorović, L., Stamenković, G., Vučetić-Tadić, B., Umezawa, K., Božović, A., Yamashita, S.,& Stanojević, B.. (2021). Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2. in Archives of Biological Sciences
Serbian Biological Society., 73(1), 31-38.
https://doi.org/10.2298/abs201010055t

Todorović L, Stamenković G, Vučetić-Tadić B, Umezawa K, Božović A, Yamashita S, Stanojević B. Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2. in Archives of Biological Sciences. 2021;73(1):31-38.
doi:10.2298/abs201010055t .

Todorović, Lidija, Stamenković, Gorana, Vučetić-Tadić, Biljana, Umezawa, Kazuo, Božović, Ana, Yamashita, Shunichi, Stanojević, Boban, "Synergistic effect of 17-allylamino-17-demethoxygeldanamycin with dehydroxymethylepoxyquinomicin on the human anaplastic thyroid carcinoma cell line KTC2" in Archives of Biological Sciences, 73, no. 1 (2021):31-38,
https://doi.org/10.2298/abs201010055t . .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Ćirović, Duško
AU  - Ćirić, Danica
AU  - Stojković, Oliver
AU  - Veličković, Jelena
AU  - Kataranovski, Dragan
AU  - Savić, Ivo
PY  - 2020
UR  - https://link.springer.com/article/10.1007/s42991-020-00019-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3660
AB  - The role of intraspecific karyotype variability in reproductive isolation and speciation has been widely studied. Among the 26 genera of Palaearctic mammals, the blind mole rats genus Nannospalax has the highest karyotype variability with 74 chromosomal forms (CFs). Although these CFs have been described in detail, taxonomic effects of chromosomal rearrangements are still lacking, especially among 25 recorded CFs of European N. leucodon superspecies. As genetic discrepancies for most of them are missing, we analyze nucleotide sequence polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs. Here we provide for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus and syrmiensis using 40–57-year-old archived samples from our mammalian collection and thus demonstrate the usefulness of archived/museum samples as starting material for DNA analysis. The topology of the phylogenetic tree is congruent with the traditional taxonomic separation of recent blind mole rats with high support. Diversification of N. leucodon cluster into discrete subclusters—CFs—and the extent of evolutionary divergence among them are in accordance with previous findings of complete reproductive isolation between six CFs analyzed here. Additionally, the level of evolutionary divergence among six N. leucodon CFs resembles those recorded among clearly distinct Spalax species and four proposed species of N. ehrenbergi. These facts suggest that they could be cryptic species and bring attention to their conservation and natural resource protection.
PB  - Springer
T2  - Mammalian Biology
T1  - 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)
VL  - 100
DO  - 10.1007/s42991-020-00019-9
SP  - 315
EP  - 324
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Ćirović, Duško and Ćirić, Danica and Stojković, Oliver and Veličković, Jelena and Kataranovski, Dragan and Savić, Ivo",
year = "2020",
abstract = "The role of intraspecific karyotype variability in reproductive isolation and speciation has been widely studied. Among the 26 genera of Palaearctic mammals, the blind mole rats genus Nannospalax has the highest karyotype variability with 74 chromosomal forms (CFs). Although these CFs have been described in detail, taxonomic effects of chromosomal rearrangements are still lacking, especially among 25 recorded CFs of European N. leucodon superspecies. As genetic discrepancies for most of them are missing, we analyze nucleotide sequence polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs. Here we provide for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus and syrmiensis using 40–57-year-old archived samples from our mammalian collection and thus demonstrate the usefulness of archived/museum samples as starting material for DNA analysis. The topology of the phylogenetic tree is congruent with the traditional taxonomic separation of recent blind mole rats with high support. Diversification of N. leucodon cluster into discrete subclusters—CFs—and the extent of evolutionary divergence among them are in accordance with previous findings of complete reproductive isolation between six CFs analyzed here. Additionally, the level of evolutionary divergence among six N. leucodon CFs resembles those recorded among clearly distinct Spalax species and four proposed species of N. ehrenbergi. These facts suggest that they could be cryptic species and bring attention to their conservation and natural resource protection.",
publisher = "Springer",
journal = "Mammalian Biology",
title = "16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)",
volume = "100",
doi = "10.1007/s42991-020-00019-9",
pages = "315-324"
}

Bugarski-Stanojević, V., Stamenković, G., Ćirović, D., Ćirić, D., Stojković, O., Veličković, J., Kataranovski, D.,& Savić, I.. (2020). 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae). in Mammalian Biology
Springer., 100, 315-324.
https://doi.org/10.1007/s42991-020-00019-9

Bugarski-Stanojević V, Stamenković G, Ćirović D, Ćirić D, Stojković O, Veličković J, Kataranovski D, Savić I. 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae). in Mammalian Biology. 2020;100:315-324.
doi:10.1007/s42991-020-00019-9 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Ćirović, Duško, Ćirić, Danica, Stojković, Oliver, Veličković, Jelena, Kataranovski, Dragan, Savić, Ivo, "16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)" in Mammalian Biology, 100 (2020):315-324,
https://doi.org/10.1007/s42991-020-00019-9 . .

TY  - JOUR
AU  - Stanojević, Maja
AU  - Li, Kun
AU  - Stamenković, Gorana
AU  - Ilić, Bojan
AU  - Paunović, Milan
AU  - Pešić, Branislav
AU  - Đurić Maslovara, Ivana
AU  - Šiljić, Marina
AU  - Ćirković, Valentina
AU  - Zhang, Yongzhen
PY  - 2020
UR  - https://www.mdpi.com/1999-4915/12/9/975
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32887342
UR  - https://radar.ibiss.bg.ac.rs/123456789/3880
AB  - Hematophagous arthropods are important vectors for zoonotic pathogens. To date, a huge number of viruses have been identified in these arthropods, with a considerable proportion of them being human pathogens. However, the viromes of hematophagous arthropods are still largely unresearched. In this study, a number of arthropods were collected from Belgrade, Serbia including mosquitoes, ticks and bedbugs. The viromes of these arthropods were identified and characterized using Illumina MiSeq sequencing. In total, 21 viruses belonging to 11 families were characterized, with 11 of them representing novel species. These results may contribute to our knowledge of RNA viruses in arthropods and the discovery of novel human pathogens.
PB  - NLM (Medline)
T2  - Viruses
T1  - Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia
IS  - 9
VL  - 12
DO  - 10.3390/v12090975
SP  - 975
ER  - 

@article{
author = "Stanojević, Maja and Li, Kun and Stamenković, Gorana and Ilić, Bojan and Paunović, Milan and Pešić, Branislav and Đurić Maslovara, Ivana and Šiljić, Marina and Ćirković, Valentina and Zhang, Yongzhen",
year = "2020",
abstract = "Hematophagous arthropods are important vectors for zoonotic pathogens. To date, a huge number of viruses have been identified in these arthropods, with a considerable proportion of them being human pathogens. However, the viromes of hematophagous arthropods are still largely unresearched. In this study, a number of arthropods were collected from Belgrade, Serbia including mosquitoes, ticks and bedbugs. The viromes of these arthropods were identified and characterized using Illumina MiSeq sequencing. In total, 21 viruses belonging to 11 families were characterized, with 11 of them representing novel species. These results may contribute to our knowledge of RNA viruses in arthropods and the discovery of novel human pathogens.",
publisher = "NLM (Medline)",
journal = "Viruses",
title = "Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia",
number = "9",
volume = "12",
doi = "10.3390/v12090975",
pages = "975"
}

Stanojević, M., Li, K., Stamenković, G., Ilić, B., Paunović, M., Pešić, B., Đurić Maslovara, I., Šiljić, M., Ćirković, V.,& Zhang, Y.. (2020). Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia. in Viruses
NLM (Medline)., 12(9), 975.
https://doi.org/10.3390/v12090975

Stanojević M, Li K, Stamenković G, Ilić B, Paunović M, Pešić B, Đurić Maslovara I, Šiljić M, Ćirković V, Zhang Y. Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia. in Viruses. 2020;12(9):975.
doi:10.3390/v12090975 .

Stanojević, Maja, Li, Kun, Stamenković, Gorana, Ilić, Bojan, Paunović, Milan, Pešić, Branislav, Đurić Maslovara, Ivana, Šiljić, Marina, Ćirković, Valentina, Zhang, Yongzhen, "Depicting the RNA Virome of Hematophagous Arthropods from Belgrade, Serbia" in Viruses, 12, no. 9 (2020):975,
https://doi.org/10.3390/v12090975 . .

TY  - JOUR
AU  - Đurović, Jelena
AU  - Stamenković, Gorana
AU  - Todorović, Jelena
AU  - Aleksić, Natasa
AU  - Stojković, Oliver
PY  - 2020
UR  - https://www.tandfonline.com/doi/full/10.1080/14647273.2018.1515503
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3141
AB  - AbstractUnexplained infertility refers to the absence of a definable cause of reproductive failure. Vitamin D receptor (VDR) acts as a transcription factor and regulates a number of vitamin D-responsive genes, including those involved in the immune system. Recent finding that VDR is expressed in reproductive tissues suggests a possible importance of vitamin D in pregnancy. We conducted a case-control study to examine the association of polymorphisms in VDR gene with reproductive success. DNA from 117 female patients with unexplained infertility and 130 fertile controls was isolated from peripheral blood and VDR genotypes (FokI, BsmI, ApaI and TaqI) were detected by PCR-RFLP. Haplotypes were determined using Haploview software. Our results show significant association of FokI and BsmI polymorphisms with infertility (p < 0.05). The haplotype analysis confirmed strong linkage disequilibrium between closely positioned BsmI, ApaI and TaqI polymorphisms. Two haplotypes were associated with infertility: (i) hapl...
T2  - Human Fertility
T1  - Polymorphisms and haplotypes in VDR gene are associated with female idiopathic infertility
IS  - 2
VL  - 23
DO  - 10.1080/14647273.2018.1515503
SP  - 101
SP  - 101
EP  - 110
EP  - 110
ER  - 

@article{
author = "Đurović, Jelena and Stamenković, Gorana and Todorović, Jelena and Aleksić, Natasa and Stojković, Oliver",
year = "2020",
abstract = "AbstractUnexplained infertility refers to the absence of a definable cause of reproductive failure. Vitamin D receptor (VDR) acts as a transcription factor and regulates a number of vitamin D-responsive genes, including those involved in the immune system. Recent finding that VDR is expressed in reproductive tissues suggests a possible importance of vitamin D in pregnancy. We conducted a case-control study to examine the association of polymorphisms in VDR gene with reproductive success. DNA from 117 female patients with unexplained infertility and 130 fertile controls was isolated from peripheral blood and VDR genotypes (FokI, BsmI, ApaI and TaqI) were detected by PCR-RFLP. Haplotypes were determined using Haploview software. Our results show significant association of FokI and BsmI polymorphisms with infertility (p < 0.05). The haplotype analysis confirmed strong linkage disequilibrium between closely positioned BsmI, ApaI and TaqI polymorphisms. Two haplotypes were associated with infertility: (i) hapl...",
journal = "Human Fertility",
title = "Polymorphisms and haplotypes in VDR gene are associated with female idiopathic infertility",
number = "2",
volume = "23",
doi = "10.1080/14647273.2018.1515503",
pages = "101-101-110-110"
}

Đurović, J., Stamenković, G., Todorović, J., Aleksić, N.,& Stojković, O.. (2020). Polymorphisms and haplotypes in VDR gene are associated with female idiopathic infertility. in Human Fertility, 23(2), 101-110.
https://doi.org/10.1080/14647273.2018.1515503

Đurović J, Stamenković G, Todorović J, Aleksić N, Stojković O. Polymorphisms and haplotypes in VDR gene are associated with female idiopathic infertility. in Human Fertility. 2020;23(2):101-110.
doi:10.1080/14647273.2018.1515503 .

Đurović, Jelena, Stamenković, Gorana, Todorović, Jelena, Aleksić, Natasa, Stojković, Oliver, "Polymorphisms and haplotypes in VDR gene are associated with female idiopathic infertility" in Human Fertility, 23, no. 2 (2020):101-110,
https://doi.org/10.1080/14647273.2018.1515503 . .

TY  - CONF
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Ćirović, Duško
AU  - Ćirić, Danica
AU  - Stojković, Oliver
AU  - Veličković, Jelena
AU  - Kataranovski, Dragan
AU  - Savić, Ivo
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5657
AB  - Among 26 genera of Palaearctic mammals, the genus Nannospalax has the highest karyotype
variability with 74 chromosomal forms (CFs). Taxonomic effects i.e. implications to phylogeny
and speciation process of such chromosomal variety are still lacking, especially among 25
reported CFs of South-European N. leucodon superspecies. Many cryptic species are under
serious threat of complete disappearance, with population declines in Europe. As genetic
discrepancies for the majority of them are missing, we analyzed nucleotide sequence
polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs and also
add for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus
and syrmiensis. Further, including 40-57 years old teeth we evaluate the usefulness of the
archived samples, e.g. from museums and other old collections as starting material for
phylogenetic analysis. The topology of the Bayesian Inference tree is in agreement with the
traditional taxonomic separation of recent blind mole rats. Among the three superspecies, the
genetic diversity was lowest in N. ehrenbergi (0.004-0.031), highest in N. xanthodon (0.009-
0.063) and intermediate in N. leucodon (0.008-0.055). The comparable scale of evolutionary
divergence among N. leucodon CFs and among species from the genus Spalax supports our
previous proposal that seven reproductively isolated CFs should be considered to be cryptic
species and thus protected from extinction in their natural habitat.
PB  - Belgrade: Serbian Genetic Society
C3  - 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
T1  - 16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae)
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5657
ER  - 

@conference{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Ćirović, Duško and Ćirić, Danica and Stojković, Oliver and Veličković, Jelena and Kataranovski, Dragan and Savić, Ivo",
year = "2019",
abstract = "Among 26 genera of Palaearctic mammals, the genus Nannospalax has the highest karyotype
variability with 74 chromosomal forms (CFs). Taxonomic effects i.e. implications to phylogeny
and speciation process of such chromosomal variety are still lacking, especially among 25
reported CFs of South-European N. leucodon superspecies. Many cryptic species are under
serious threat of complete disappearance, with population declines in Europe. As genetic
discrepancies for the majority of them are missing, we analyzed nucleotide sequence
polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs and also
add for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus
and syrmiensis. Further, including 40-57 years old teeth we evaluate the usefulness of the
archived samples, e.g. from museums and other old collections as starting material for
phylogenetic analysis. The topology of the Bayesian Inference tree is in agreement with the
traditional taxonomic separation of recent blind mole rats. Among the three superspecies, the
genetic diversity was lowest in N. ehrenbergi (0.004-0.031), highest in N. xanthodon (0.009-
0.063) and intermediate in N. leucodon (0.008-0.055). The comparable scale of evolutionary
divergence among N. leucodon CFs and among species from the genus Spalax supports our
previous proposal that seven reproductively isolated CFs should be considered to be cryptic
species and thus protected from extinction in their natural habitat.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia",
title = "16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae)",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5657"
}

Bugarski-Stanojević, V., Stamenković, G., Ćirović, D., Ćirić, D., Stojković, O., Veličković, J., Kataranovski, D.,& Savić, I.. (2019). 16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae). in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society..
https://hdl.handle.net/21.15107/rcub_ibiss_5657

Bugarski-Stanojević V, Stamenković G, Ćirović D, Ćirić D, Stojković O, Veličković J, Kataranovski D, Savić I. 16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae). in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia. 2019;.
https://hdl.handle.net/21.15107/rcub_ibiss_5657 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Ćirović, Duško, Ćirić, Danica, Stojković, Oliver, Veličković, Jelena, Kataranovski, Dragan, Savić, Ivo, "16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae)" in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia (2019),
https://hdl.handle.net/21.15107/rcub_ibiss_5657 .

TY  - JOUR
AU  - Kačar, Tamara
AU  - Stamenković, Gorana
AU  - Blagojević, Jelena
AU  - Krtinić, Jovica
AU  - Mijović, Dragan
AU  - Marjanović, Damir
PY  - 2019
UR  - https://www.tandfonline.com/doi/full/10.1080/03014460.2019.1584242
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3344
AB  - BACKGROUND Serbs mainly live in the territory of the recently re-established state of Serbia. However, the turbulent history in the Balkan Peninsula has led to settlement of Serbs not only within present day Serbia, but also in different parts of neighbouring countries. AIM To define polymorphisms of 23 Y-chromosomal short tandem repeat (STR) loci in a modern Serbian population from the central part of the Balkan Peninsula. SUBJECTS AND METHODS The reference sample consisted of 303 men declared as Serbs over three generations. Localities of the collected materials include the territories of Serbia, Bosnia and Herzegovina, Croatia and Montenegro. DNA samples were typed using the PowerPlex®Y23 amplification kit. RESULTS The highest locus diversity was observed for DYS385 and DYS481. In this study the most abundant haplogroups were I2a, E1b1b, R1a and I1. The largest genetic distances between the Serbs and other close Southern Slavs were for the Macedonians and Slovenians. CONCLUSION This study is the first one to define STR polymorphism of Serbian people not only from Serbia but also from other parts of the Balkan Peninsula. The presented genetic data may be useful in further examinations of the genesis and genetic structuring of the present-day Serbian gene pool.
T2  - Annals of Human Biology
T1  - Y chromosome genetic data defined by 23 short tandem repeats in a Serbian population on the Balkan Peninsula.
IS  - 1
VL  - 46
DO  - 10.1080/03014460.2019.1584242
SP  - 77
EP  - 83
ER  - 

@article{
author = "Kačar, Tamara and Stamenković, Gorana and Blagojević, Jelena and Krtinić, Jovica and Mijović, Dragan and Marjanović, Damir",
year = "2019",
abstract = "BACKGROUND Serbs mainly live in the territory of the recently re-established state of Serbia. However, the turbulent history in the Balkan Peninsula has led to settlement of Serbs not only within present day Serbia, but also in different parts of neighbouring countries. AIM To define polymorphisms of 23 Y-chromosomal short tandem repeat (STR) loci in a modern Serbian population from the central part of the Balkan Peninsula. SUBJECTS AND METHODS The reference sample consisted of 303 men declared as Serbs over three generations. Localities of the collected materials include the territories of Serbia, Bosnia and Herzegovina, Croatia and Montenegro. DNA samples were typed using the PowerPlex®Y23 amplification kit. RESULTS The highest locus diversity was observed for DYS385 and DYS481. In this study the most abundant haplogroups were I2a, E1b1b, R1a and I1. The largest genetic distances between the Serbs and other close Southern Slavs were for the Macedonians and Slovenians. CONCLUSION This study is the first one to define STR polymorphism of Serbian people not only from Serbia but also from other parts of the Balkan Peninsula. The presented genetic data may be useful in further examinations of the genesis and genetic structuring of the present-day Serbian gene pool.",
journal = "Annals of Human Biology",
title = "Y chromosome genetic data defined by 23 short tandem repeats in a Serbian population on the Balkan Peninsula.",
number = "1",
volume = "46",
doi = "10.1080/03014460.2019.1584242",
pages = "77-83"
}

Kačar, T., Stamenković, G., Blagojević, J., Krtinić, J., Mijović, D.,& Marjanović, D.. (2019). Y chromosome genetic data defined by 23 short tandem repeats in a Serbian population on the Balkan Peninsula.. in Annals of Human Biology, 46(1), 77-83.
https://doi.org/10.1080/03014460.2019.1584242

Kačar T, Stamenković G, Blagojević J, Krtinić J, Mijović D, Marjanović D. Y chromosome genetic data defined by 23 short tandem repeats in a Serbian population on the Balkan Peninsula.. in Annals of Human Biology. 2019;46(1):77-83.
doi:10.1080/03014460.2019.1584242 .

Kačar, Tamara, Stamenković, Gorana, Blagojević, Jelena, Krtinić, Jovica, Mijović, Dragan, Marjanović, Damir, "Y chromosome genetic data defined by 23 short tandem repeats in a Serbian population on the Balkan Peninsula." in Annals of Human Biology, 46, no. 1 (2019):77-83,
https://doi.org/10.1080/03014460.2019.1584242 . .

TY  - JOUR
AU  - Veličković, Jelena
AU  - Zeljić, Katarina
AU  - Todorovic, Jelena
AU  - Stamenković, Gorana
AU  - Stojković, Oliver
PY  - 2019
UR  - https://insights.ovid.com/crossref?an=00001721-201909000-00001
UR  - http://insights.ovid.com/crossref?an=00001721-201909000-00001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3457
UR  - https://journals.lww.com/bloodcoagulation/Abstract/2019/09000/Hemostasis_related_gene_polymorphisms_and_their.1.aspx
AB  - A numerous factor can cause infertility, but around one of four reproductive failure cases remain unexplained and diagnosed as idiopathic infertility. In the past few decades, analysis of gene polymorphisms takes a significant place in pathogenesis of infertility. The aim of this study was to evaluate the possible role of hemostasis-related gene polymorphisms in unexplained infertility. The study includes 117 female patients with idiopathic infertility and 130 fertile women with at least one born child. Eight polymorphisms important for hemostasis (ITGB3 1565T>C, FV 1691G>A, FII 20210G>A, MTHFR 677C>T and 1298A>C, ATIII 786G>A, PAI-14G/5G and ACE I/D) were genotyped by real-time PCR system. The frequencies of alleles and genotypes of examined polymorphisms were analyzed in SPSS statistical program, whereas gene interactions were identified using the GMDR software. Examination of etiological factors has shown that family history is a significant factor in assessing individual risk for infertility. The alleles and genotypes frequency of FV 1691G>A and FII 20210G>A polymorphisms were statistically different between control and patient group leading to a greater risk for infertility. The analysis of epistatic relationship between examined hemostasis-related gene polymorphisms identified more complex high-risk genotypes associated with infertility. Our results suggest that positive family history could be important predictive factor for fertility problems, pointing to the potential hereditary basis of this condition. Polymorphisms FVL and FII prothrombin are independent risk factors for idiopathic infertility, whereas multilocus interactions approach should be taken into consideration for the future research.
T2  - Blood Coagulation & Fibrinolysis
T1  - Hemostasis-related gene polymorphisms and their epistatic relationship in women with idiopathic infertility
IS  - 6
VL  - 30
DO  - 10.1097/MBC.0000000000000830
SP  - 253
EP  - 262
ER  - 

@article{
author = "Veličković, Jelena and Zeljić, Katarina and Todorovic, Jelena and Stamenković, Gorana and Stojković, Oliver",
year = "2019",
abstract = "A numerous factor can cause infertility, but around one of four reproductive failure cases remain unexplained and diagnosed as idiopathic infertility. In the past few decades, analysis of gene polymorphisms takes a significant place in pathogenesis of infertility. The aim of this study was to evaluate the possible role of hemostasis-related gene polymorphisms in unexplained infertility. The study includes 117 female patients with idiopathic infertility and 130 fertile women with at least one born child. Eight polymorphisms important for hemostasis (ITGB3 1565T>C, FV 1691G>A, FII 20210G>A, MTHFR 677C>T and 1298A>C, ATIII 786G>A, PAI-14G/5G and ACE I/D) were genotyped by real-time PCR system. The frequencies of alleles and genotypes of examined polymorphisms were analyzed in SPSS statistical program, whereas gene interactions were identified using the GMDR software. Examination of etiological factors has shown that family history is a significant factor in assessing individual risk for infertility. The alleles and genotypes frequency of FV 1691G>A and FII 20210G>A polymorphisms were statistically different between control and patient group leading to a greater risk for infertility. The analysis of epistatic relationship between examined hemostasis-related gene polymorphisms identified more complex high-risk genotypes associated with infertility. Our results suggest that positive family history could be important predictive factor for fertility problems, pointing to the potential hereditary basis of this condition. Polymorphisms FVL and FII prothrombin are independent risk factors for idiopathic infertility, whereas multilocus interactions approach should be taken into consideration for the future research.",
journal = "Blood Coagulation & Fibrinolysis",
title = "Hemostasis-related gene polymorphisms and their epistatic relationship in women with idiopathic infertility",
number = "6",
volume = "30",
doi = "10.1097/MBC.0000000000000830",
pages = "253-262"
}

Veličković, J., Zeljić, K., Todorovic, J., Stamenković, G.,& Stojković, O.. (2019). Hemostasis-related gene polymorphisms and their epistatic relationship in women with idiopathic infertility. in Blood Coagulation & Fibrinolysis, 30(6), 253-262.
https://doi.org/10.1097/MBC.0000000000000830

Veličković J, Zeljić K, Todorovic J, Stamenković G, Stojković O. Hemostasis-related gene polymorphisms and their epistatic relationship in women with idiopathic infertility. in Blood Coagulation & Fibrinolysis. 2019;30(6):253-262.
doi:10.1097/MBC.0000000000000830 .

Veličković, Jelena, Zeljić, Katarina, Todorovic, Jelena, Stamenković, Gorana, Stojković, Oliver, "Hemostasis-related gene polymorphisms and their epistatic relationship in women with idiopathic infertility" in Blood Coagulation & Fibrinolysis, 30, no. 6 (2019):253-262,
https://doi.org/10.1097/MBC.0000000000000830 . .

TY  - JOUR
AU  - Stanojević, Maja
AU  - Ćirković, Valentina
AU  - Siljić, Marina
AU  - Gligić, Ana
AU  - Stamenković, Gorana
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S187603411930108X?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3316
AB  - BACKGROUND Hantaviruses are etiological agents of emerging zoonotic diseases worldwide, including hemorrhagic fever with renal syndrome (HFRS). A number of hantavirus species is known to be present in Europe. In Serbia, existing data on hantavirus presence and prevalence rely in serological findings. In this study, molecular analysis was performed in order to characterize HFRS causing hantaviruses in Serbia. METHODS Sixty four serum samples of HFRS cases, previously found seropositive to anti-hantaviral antibodies, were included in the study. Partial hantaviral L and S segments were PCR amplified producing 390nt and 598nt amplicons, respectively, in parallel with human beta-actin mRNA as external reverse transcription positive control. Hantavirus specific PCR products were DNA sequenced in both direction and the obtained sequences phylogenetically confirmed and analyzed. RESULTS PCR detection of hantavirus L and S genome segments was positive in 18/64 and 11/64 tested samples, respectively. Positive PCR results involved samples obtained from different locations, mostly from central and southern parts of Serbia. All the obtained sequences were identified as Dobrava-Belgrade virus (DOBV). In the phylogenetic analysis sequences from Serbia tended to cluster in distinctive, geographically related clusters. CONCLUSIONS Our findings indicate DOBV as the main HFRS causing hantavirus in Serbia, the site of its initial isolation.
T2  - Journal of Infection and Public Health
T1  - Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011.
DO  - 10.1016/j.jiph.2019.02.021
ER  - 

@article{
author = "Stanojević, Maja and Ćirković, Valentina and Siljić, Marina and Gligić, Ana and Stamenković, Gorana",
year = "2019",
abstract = "BACKGROUND Hantaviruses are etiological agents of emerging zoonotic diseases worldwide, including hemorrhagic fever with renal syndrome (HFRS). A number of hantavirus species is known to be present in Europe. In Serbia, existing data on hantavirus presence and prevalence rely in serological findings. In this study, molecular analysis was performed in order to characterize HFRS causing hantaviruses in Serbia. METHODS Sixty four serum samples of HFRS cases, previously found seropositive to anti-hantaviral antibodies, were included in the study. Partial hantaviral L and S segments were PCR amplified producing 390nt and 598nt amplicons, respectively, in parallel with human beta-actin mRNA as external reverse transcription positive control. Hantavirus specific PCR products were DNA sequenced in both direction and the obtained sequences phylogenetically confirmed and analyzed. RESULTS PCR detection of hantavirus L and S genome segments was positive in 18/64 and 11/64 tested samples, respectively. Positive PCR results involved samples obtained from different locations, mostly from central and southern parts of Serbia. All the obtained sequences were identified as Dobrava-Belgrade virus (DOBV). In the phylogenetic analysis sequences from Serbia tended to cluster in distinctive, geographically related clusters. CONCLUSIONS Our findings indicate DOBV as the main HFRS causing hantavirus in Serbia, the site of its initial isolation.",
journal = "Journal of Infection and Public Health",
title = "Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011.",
doi = "10.1016/j.jiph.2019.02.021"
}

Stanojević, M., Ćirković, V., Siljić, M., Gligić, A.,& Stamenković, G.. (2019). Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011.. in Journal of Infection and Public Health.
https://doi.org/10.1016/j.jiph.2019.02.021

Stanojević M, Ćirković V, Siljić M, Gligić A, Stamenković G. Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011.. in Journal of Infection and Public Health. 2019;.
doi:10.1016/j.jiph.2019.02.021 .

Stanojević, Maja, Ćirković, Valentina, Siljić, Marina, Gligić, Ana, Stamenković, Gorana, "Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011." in Journal of Infection and Public Health (2019),
https://doi.org/10.1016/j.jiph.2019.02.021 . .

TY  - JOUR
AU  - Li, Kun
AU  - Stanojević, Maja
AU  - Stamenković, Gorana
AU  - Ilić, Bojan
AU  - Paunović, Milan
AU  - Lu, Miao
AU  - Pešić, Branislav
AU  - Đurić Maslovara, Ivana
AU  - Siljic, Marina
AU  - Cirkovic, Valentina
AU  - Zhang, Yongzhen
PY  - 2019
UR  - http://www.nature.com/articles/s41598-019-55077-y
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6904564
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3575
AB  - Rickettsiales bacteria in arthropods play a significant role in both public health and arthropod ecology. However, the extensive genetic diversity of Rickettsiales endosymbionts of arthropods is still to be discovered. In 2016, 515 arthropods belonging to 9 species of four classes (Insecta, Chilopoda, Diplopoda and Arachnida) were collected in Serbia. The presence and genetic diversity of Rickettsiales bacteria were evaluated by characterizing the 16S rRNA (rrs), citrate synthase (gltA) and heat shock protein (groEL) genes. The presence of various Rickettsiales bacteria was identified in the majority of tested arthropod species. The results revealed co-circulation of five recognized Rickettsiales species including Rickettsia, Ehrlichia and Wolbachia, as well as four tentative novel species, including one tentative novel genus named Neowolbachia. These results suggest the remarkable genetic diversity of Rickettsiales bacteria in certain arthropod species in this region. Furthermore, the high prevalence of spotted fever group Rickettsia in Ixodes ricinus ticks highlights the potential public health risk of human Rickettsia infection.
PB  - Nature Publishing Group
T2  - Scientific Reports
T1  - Insight into diversity of bacteria belonging to the order Rickettsiales in 9 arthropods species collected in Serbia.
IS  - 1
VL  - 9
DO  - 10.1038/s41598-019-55077-y
SP  - 18680
ER  - 

@article{
author = "Li, Kun and Stanojević, Maja and Stamenković, Gorana and Ilić, Bojan and Paunović, Milan and Lu, Miao and Pešić, Branislav and Đurić Maslovara, Ivana and Siljic, Marina and Cirkovic, Valentina and Zhang, Yongzhen",
year = "2019",
abstract = "Rickettsiales bacteria in arthropods play a significant role in both public health and arthropod ecology. However, the extensive genetic diversity of Rickettsiales endosymbionts of arthropods is still to be discovered. In 2016, 515 arthropods belonging to 9 species of four classes (Insecta, Chilopoda, Diplopoda and Arachnida) were collected in Serbia. The presence and genetic diversity of Rickettsiales bacteria were evaluated by characterizing the 16S rRNA (rrs), citrate synthase (gltA) and heat shock protein (groEL) genes. The presence of various Rickettsiales bacteria was identified in the majority of tested arthropod species. The results revealed co-circulation of five recognized Rickettsiales species including Rickettsia, Ehrlichia and Wolbachia, as well as four tentative novel species, including one tentative novel genus named Neowolbachia. These results suggest the remarkable genetic diversity of Rickettsiales bacteria in certain arthropod species in this region. Furthermore, the high prevalence of spotted fever group Rickettsia in Ixodes ricinus ticks highlights the potential public health risk of human Rickettsia infection.",
publisher = "Nature Publishing Group",
journal = "Scientific Reports",
title = "Insight into diversity of bacteria belonging to the order Rickettsiales in 9 arthropods species collected in Serbia.",
number = "1",
volume = "9",
doi = "10.1038/s41598-019-55077-y",
pages = "18680"
}

Li, K., Stanojević, M., Stamenković, G., Ilić, B., Paunović, M., Lu, M., Pešić, B., Đurić Maslovara, I., Siljic, M., Cirkovic, V.,& Zhang, Y.. (2019). Insight into diversity of bacteria belonging to the order Rickettsiales in 9 arthropods species collected in Serbia.. in Scientific Reports
Nature Publishing Group., 9(1), 18680.
https://doi.org/10.1038/s41598-019-55077-y

Li K, Stanojević M, Stamenković G, Ilić B, Paunović M, Lu M, Pešić B, Đurić Maslovara I, Siljic M, Cirkovic V, Zhang Y. Insight into diversity of bacteria belonging to the order Rickettsiales in 9 arthropods species collected in Serbia.. in Scientific Reports. 2019;9(1):18680.
doi:10.1038/s41598-019-55077-y .

Li, Kun, Stanojević, Maja, Stamenković, Gorana, Ilić, Bojan, Paunović, Milan, Lu, Miao, Pešić, Branislav, Đurić Maslovara, Ivana, Siljic, Marina, Cirkovic, Valentina, Zhang, Yongzhen, "Insight into diversity of bacteria belonging to the order Rickettsiales in 9 arthropods species collected in Serbia." in Scientific Reports, 9, no. 1 (2019):18680,
https://doi.org/10.1038/s41598-019-55077-y . .

TY  - JOUR
AU  - Čubrić, Tijana
AU  - Stamenković, Gorana
AU  - Ilić, Marija
AU  - Crnobrnja-Isailović, Jelka
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641900028C
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/3548
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3572
AB  - Seven genetic clades have been recognized within the species Vipera ammodytes (nose-horned viper); however, the precise phylogenetic position of many Balkan populations is unknown. We used Bayesian analysis of the mtDNA sequences from the 16S rRNA mtDNA gene obtained from 47 individuals (26 novel samples sequenced in this study and 21 sequences available from GenBank). Our results show that sampled nose-horned vipers from localities in Serbia are clustered within three clades: the northeastern (23 individuals), the northwestern (two individuals) and the southeastern (one individual). Results revealed an overlapping distribution of the northeastern and the northwestern clades in two populations. We have revealed that the northeastern clade extends further south than previously suggested, to the Ohrid/Prespa lakes in North Macedonia. Our findings contribute to the knowledge of the genetic diversity of this species in Serbia and help to clarify the geographical distributions of mtDNA-defined clades
T2  - Archives of Biological Sciences
T1  - Contribution to the phylogeography of the nose-horned viper (Vipera ammodytes, Linnaeus, 1758) in the Central Balkan Peninsula
IS  - 3
VL  - 71
DO  - 10.2298/ABS181020028C
SP  - 463
EP  - 468
ER  - 

@article{
author = "Čubrić, Tijana and Stamenković, Gorana and Ilić, Marija and Crnobrnja-Isailović, Jelka",
year = "2019",
abstract = "Seven genetic clades have been recognized within the species Vipera ammodytes (nose-horned viper); however, the precise phylogenetic position of many Balkan populations is unknown. We used Bayesian analysis of the mtDNA sequences from the 16S rRNA mtDNA gene obtained from 47 individuals (26 novel samples sequenced in this study and 21 sequences available from GenBank). Our results show that sampled nose-horned vipers from localities in Serbia are clustered within three clades: the northeastern (23 individuals), the northwestern (two individuals) and the southeastern (one individual). Results revealed an overlapping distribution of the northeastern and the northwestern clades in two populations. We have revealed that the northeastern clade extends further south than previously suggested, to the Ohrid/Prespa lakes in North Macedonia. Our findings contribute to the knowledge of the genetic diversity of this species in Serbia and help to clarify the geographical distributions of mtDNA-defined clades",
journal = "Archives of Biological Sciences",
title = "Contribution to the phylogeography of the nose-horned viper (Vipera ammodytes, Linnaeus, 1758) in the Central Balkan Peninsula",
number = "3",
volume = "71",
doi = "10.2298/ABS181020028C",
pages = "463-468"
}

Čubrić, T., Stamenković, G., Ilić, M.,& Crnobrnja-Isailović, J.. (2019). Contribution to the phylogeography of the nose-horned viper (Vipera ammodytes, Linnaeus, 1758) in the Central Balkan Peninsula. in Archives of Biological Sciences, 71(3), 463-468.
https://doi.org/10.2298/ABS181020028C

Čubrić T, Stamenković G, Ilić M, Crnobrnja-Isailović J. Contribution to the phylogeography of the nose-horned viper (Vipera ammodytes, Linnaeus, 1758) in the Central Balkan Peninsula. in Archives of Biological Sciences. 2019;71(3):463-468.
doi:10.2298/ABS181020028C .

Čubrić, Tijana, Stamenković, Gorana, Ilić, Marija, Crnobrnja-Isailović, Jelka, "Contribution to the phylogeography of the nose-horned viper (Vipera ammodytes, Linnaeus, 1758) in the Central Balkan Peninsula" in Archives of Biological Sciences, 71, no. 3 (2019):463-468,
https://doi.org/10.2298/ABS181020028C . .

TY  - JOUR
AU  - Ilić, Marija
AU  - Jojić, Vida
AU  - Stamenković, Gorana
AU  - Marković, Vanja
AU  - Simić, Vladica
AU  - Paunović, Momir
AU  - Crnobrnja-Isailović, Jelka
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3383
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3515
AB  - We conducted a comparative (2D landmark-based geometric and traditional) morphometric analysis on tadpoles at early developmental stages. Two species of brown frog (Rana dalmatina and R. temporaria) and the common toad (Bufo bufo) were involved, all raised in the laboratory from fertilized eggs collected in their natural habitat. Taxonomic identification was confirmed by the DNA barcoding method with the 16S rRNA sequence as the gene marker. Interested to compare the methodologies for quantification and description of morphological differences among tadpoles of mentioned species, we aimed to: 1) calculate interspecies genetic distances as the most relevant measurement for species differentiation, 2) determine and describe size and shape variation, 3) identify relationships among the analyzed species at the morphological level and 4) assess their classification accuracy. Within the framework of the specified aims, both methodologies produced very similar results, i.e., the smallest divergence was between R. dalmatina and R. temporaria, while the most discriminative were B. bufo and R. temporaria. However, we observed subtle shape variation of the distal region of the tail that was detected only by the geometric morphometrics. Our findings support the following. Geometric morphometric method captures more subtle shape differences that were unable to be recovered from linear measurements. It performs slightly better in classification rate. Although it was not quantified, it stands to reason that there is no difference in time investment between the two approaches. Geometric morphometrics provides more information that can be leveraged to answer further questions and it has a clear advantage in visualizing.
T2  - Amphibia-Reptilia
T1  - Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages
IS  - 4
VL  - 40
DO  - 10.1163/15685381-00001193
SP  - 499
EP  - 509
ER  - 

@article{
author = "Ilić, Marija and Jojić, Vida and Stamenković, Gorana and Marković, Vanja and Simić, Vladica and Paunović, Momir and Crnobrnja-Isailović, Jelka",
year = "2019",
abstract = "We conducted a comparative (2D landmark-based geometric and traditional) morphometric analysis on tadpoles at early developmental stages. Two species of brown frog (Rana dalmatina and R. temporaria) and the common toad (Bufo bufo) were involved, all raised in the laboratory from fertilized eggs collected in their natural habitat. Taxonomic identification was confirmed by the DNA barcoding method with the 16S rRNA sequence as the gene marker. Interested to compare the methodologies for quantification and description of morphological differences among tadpoles of mentioned species, we aimed to: 1) calculate interspecies genetic distances as the most relevant measurement for species differentiation, 2) determine and describe size and shape variation, 3) identify relationships among the analyzed species at the morphological level and 4) assess their classification accuracy. Within the framework of the specified aims, both methodologies produced very similar results, i.e., the smallest divergence was between R. dalmatina and R. temporaria, while the most discriminative were B. bufo and R. temporaria. However, we observed subtle shape variation of the distal region of the tail that was detected only by the geometric morphometrics. Our findings support the following. Geometric morphometric method captures more subtle shape differences that were unable to be recovered from linear measurements. It performs slightly better in classification rate. Although it was not quantified, it stands to reason that there is no difference in time investment between the two approaches. Geometric morphometrics provides more information that can be leveraged to answer further questions and it has a clear advantage in visualizing.",
journal = "Amphibia-Reptilia",
title = "Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages",
number = "4",
volume = "40",
doi = "10.1163/15685381-00001193",
pages = "499-509"
}

Ilić, M., Jojić, V., Stamenković, G., Marković, V., Simić, V., Paunović, M.,& Crnobrnja-Isailović, J.. (2019). Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages. in Amphibia-Reptilia, 40(4), 499-509.
https://doi.org/10.1163/15685381-00001193

Ilić M, Jojić V, Stamenković G, Marković V, Simić V, Paunović M, Crnobrnja-Isailović J. Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages. in Amphibia-Reptilia. 2019;40(4):499-509.
doi:10.1163/15685381-00001193 .

Ilić, Marija, Jojić, Vida, Stamenković, Gorana, Marković, Vanja, Simić, Vladica, Paunović, Momir, Crnobrnja-Isailović, Jelka, "Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages" in Amphibia-Reptilia, 40, no. 4 (2019):499-509,
https://doi.org/10.1163/15685381-00001193 . .

TY  - JOUR
AU  - Ilić, Marija
AU  - Jojić, Vida
AU  - Stamenković, Gorana
AU  - Marković, Vanja
AU  - Simić, Vladica
AU  - Paunović, Momir
AU  - Crnobrnja-Isailović, Jelka
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3383
AB  - We conducted a comparative (2D landmark-based geometric and traditional) morphometric analysis on tadpoles at early developmental stages. Two species of brown frog (Rana dalmatina and R. temporaria) and the common toad (Bufo bufo) were involved, all raised in the laboratory from fertilized eggs collected in their natural habitat. Taxonomic identification was confirmed by the DNA barcoding method with the 16S rRNA sequence as the gene marker. Interested to compare the methodologies for quantification and description of morphological differences among tadpoles of mentioned species, we aimed to: 1) calculate interspecies genetic distances as the most relevant measurement for species differentiation, 2) determine and describe size and shape variation, 3) identify relationships among the analyzed species at the morphological level and 4) assess their classification accuracy. Within the framework of the specified aims, both methodologies produced very similar results, i.e., the smallest divergence was between R. dalmatina and R. temporaria, while the most discriminative were B. bufo and R. temporaria. However, we observed subtle shape variation of the distal region of the tail that was detected only by the geometric morphometrics. Our findings support the following. Geometric morphometric method captures more subtle shape differences that were unable to be recovered from linear measurements. It performs slightly better in classification rate. Although it was not quantified, it stands to reason that there is no difference in time investment between the two approaches. Geometric morphometrics provides more information that can be leveraged to answer further questions and it has a clear advantage in visualizing.
T2  - Amphibia-Reptilia
T1  - Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages
IS  - 4
VL  - 40
DO  - 10.1163/15685381-00001193
SP  - 499
EP  - 509
ER  - 

@article{
author = "Ilić, Marija and Jojić, Vida and Stamenković, Gorana and Marković, Vanja and Simić, Vladica and Paunović, Momir and Crnobrnja-Isailović, Jelka",
year = "2019",
abstract = "We conducted a comparative (2D landmark-based geometric and traditional) morphometric analysis on tadpoles at early developmental stages. Two species of brown frog (Rana dalmatina and R. temporaria) and the common toad (Bufo bufo) were involved, all raised in the laboratory from fertilized eggs collected in their natural habitat. Taxonomic identification was confirmed by the DNA barcoding method with the 16S rRNA sequence as the gene marker. Interested to compare the methodologies for quantification and description of morphological differences among tadpoles of mentioned species, we aimed to: 1) calculate interspecies genetic distances as the most relevant measurement for species differentiation, 2) determine and describe size and shape variation, 3) identify relationships among the analyzed species at the morphological level and 4) assess their classification accuracy. Within the framework of the specified aims, both methodologies produced very similar results, i.e., the smallest divergence was between R. dalmatina and R. temporaria, while the most discriminative were B. bufo and R. temporaria. However, we observed subtle shape variation of the distal region of the tail that was detected only by the geometric morphometrics. Our findings support the following. Geometric morphometric method captures more subtle shape differences that were unable to be recovered from linear measurements. It performs slightly better in classification rate. Although it was not quantified, it stands to reason that there is no difference in time investment between the two approaches. Geometric morphometrics provides more information that can be leveraged to answer further questions and it has a clear advantage in visualizing.",
journal = "Amphibia-Reptilia",
title = "Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages",
number = "4",
volume = "40",
doi = "10.1163/15685381-00001193",
pages = "499-509"
}

Ilić, M., Jojić, V., Stamenković, G., Marković, V., Simić, V., Paunović, M.,& Crnobrnja-Isailović, J.. (2019). Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages. in Amphibia-Reptilia, 40(4), 499-509.
https://doi.org/10.1163/15685381-00001193

Ilić M, Jojić V, Stamenković G, Marković V, Simić V, Paunović M, Crnobrnja-Isailović J. Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages. in Amphibia-Reptilia. 2019;40(4):499-509.
doi:10.1163/15685381-00001193 .

Ilić, Marija, Jojić, Vida, Stamenković, Gorana, Marković, Vanja, Simić, Vladica, Paunović, Momir, Crnobrnja-Isailović, Jelka, "Geometric vs. traditional morphometric methods for exploring morphological variation of tadpoles at early developmental stages" in Amphibia-Reptilia, 40, no. 4 (2019):499-509,
https://doi.org/10.1163/15685381-00001193 . .

TY  - JOUR
AU  - Đurović, Jelena
AU  - Stojković, Oliver
AU  - Todorović, Jelena
AU  - Savić, Kristina
AU  - Stamenković, Gorana
PY  - 2017
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0534-00121702377D
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2904
AB  - Methylenetetrahydrofolate reductase (MTHFR) plays a critical role in the folate metabolism. The polymorphism 677C > T of the MTHFR gene, producing thermolabile enzyme with decreased function, is widely studied and associated with many conditions. Additionally, it has been shown that another polymorphism, 1298A > C, also reduces the activity of this enzyme, although to a lesser extent. The aim of this study is to evaluate the clinical informativeness of testing both MTHFR polymorphisms. Genomic DNA, were extracted from peripheral blood of 180 female patients with pregnancy complications and 183 healthy female controls, and genotyped for MTHFR 677C > T and 1298A > C loci, using TaqMan assays. Our study found similar frequency of alleles and genotypes between two groups. Based on MTHFR 677C > T genotype, 11.7% of patients homozygous for this mutation were under the possible risk. When the position 1298 was included in the testing, 22.8% of the patients were heterozygous for both polymorphisms. Additionally, 8.9% of the patients were homozygous only for the MTHFR 1298 mutation. Although, there was no differences compared to healthy control (p > 0.05), 43% of patients were found to have elevated risk which is about four time higher than results with only MTHFR 677C > T genotyping. After obtaining information for the 677 position, testing for the second polymorphism (1298A > C) should be considered, since we have shown that it dramatically increases the rate of detection of patients who are potentially at risk for MTHFR associated conditions.
T2  - Genetika
T2  - Genetika
T1  - Should MTHFR 1298 A>C be tested together with MTHFR 677 C>T polymorphism in women with reproductive challenges?
IS  - 2
VL  - 49
DO  - 10.2298/GENSR1702377D
SP  - 377
EP  - 386
ER  - 

@article{
author = "Đurović, Jelena and Stojković, Oliver and Todorović, Jelena and Savić, Kristina and Stamenković, Gorana",
year = "2017",
abstract = "Methylenetetrahydrofolate reductase (MTHFR) plays a critical role in the folate metabolism. The polymorphism 677C > T of the MTHFR gene, producing thermolabile enzyme with decreased function, is widely studied and associated with many conditions. Additionally, it has been shown that another polymorphism, 1298A > C, also reduces the activity of this enzyme, although to a lesser extent. The aim of this study is to evaluate the clinical informativeness of testing both MTHFR polymorphisms. Genomic DNA, were extracted from peripheral blood of 180 female patients with pregnancy complications and 183 healthy female controls, and genotyped for MTHFR 677C > T and 1298A > C loci, using TaqMan assays. Our study found similar frequency of alleles and genotypes between two groups. Based on MTHFR 677C > T genotype, 11.7% of patients homozygous for this mutation were under the possible risk. When the position 1298 was included in the testing, 22.8% of the patients were heterozygous for both polymorphisms. Additionally, 8.9% of the patients were homozygous only for the MTHFR 1298 mutation. Although, there was no differences compared to healthy control (p > 0.05), 43% of patients were found to have elevated risk which is about four time higher than results with only MTHFR 677C > T genotyping. After obtaining information for the 677 position, testing for the second polymorphism (1298A > C) should be considered, since we have shown that it dramatically increases the rate of detection of patients who are potentially at risk for MTHFR associated conditions.",
journal = "Genetika, Genetika",
title = "Should MTHFR 1298 A>C be tested together with MTHFR 677 C>T polymorphism in women with reproductive challenges?",
number = "2",
volume = "49",
doi = "10.2298/GENSR1702377D",
pages = "377-386"
}

Đurović, J., Stojković, O., Todorović, J., Savić, K.,& Stamenković, G.. (2017). Should MTHFR 1298 A>C be tested together with MTHFR 677 C>T polymorphism in women with reproductive challenges?. in Genetika, 49(2), 377-386.
https://doi.org/10.2298/GENSR1702377D

Đurović J, Stojković O, Todorović J, Savić K, Stamenković G. Should MTHFR 1298 A>C be tested together with MTHFR 677 C>T polymorphism in women with reproductive challenges?. in Genetika. 2017;49(2):377-386.
doi:10.2298/GENSR1702377D .

Đurović, Jelena, Stojković, Oliver, Todorović, Jelena, Savić, Kristina, Stamenković, Gorana, "Should MTHFR 1298 A>C be tested together with MTHFR 677 C>T polymorphism in women with reproductive challenges?" in Genetika, 49, no. 2 (2017):377-386,
https://doi.org/10.2298/GENSR1702377D . .

TY  - JOUR
AU  - Ćirković, Valentina
AU  - Stamenković, Gorana
AU  - Jovanović, Jelena
AU  - Šiljić, Marina
AU  - Paunović, Milan
AU  - Stanojević, Maja
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2547
AB  - Bats represent a known reservoir of emerging viruses, yet no molecular data are found about the occurrence of zoonotic viruses in bats in the Balkans. The aim of this study was to determine the presence of paramyxo- and hanta-viruses in bats, examined by PCR in 95 deceased bats, that were collected in Serbia and Montenegro, during the period 2002 to 2009. All samples tested positive for beta-actin mRNA, confirming successful RNA isolation and amplification. However, no sample tested positive for virus specific RNA. Our findings might reflect tissue degradation in carcass samples and do not exclude bats as potential viral reservoir in the surveyed geographic area.
T2  - Tropical Biomedicine
T1  - Failure to detect viral RNA in bat samples collected in the Balkan region
IS  - 4
VL  - 33
SP  - 780
EP  - 785
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2547
ER  - 

@article{
author = "Ćirković, Valentina and Stamenković, Gorana and Jovanović, Jelena and Šiljić, Marina and Paunović, Milan and Stanojević, Maja",
year = "2016",
abstract = "Bats represent a known reservoir of emerging viruses, yet no molecular data are found about the occurrence of zoonotic viruses in bats in the Balkans. The aim of this study was to determine the presence of paramyxo- and hanta-viruses in bats, examined by PCR in 95 deceased bats, that were collected in Serbia and Montenegro, during the period 2002 to 2009. All samples tested positive for beta-actin mRNA, confirming successful RNA isolation and amplification. However, no sample tested positive for virus specific RNA. Our findings might reflect tissue degradation in carcass samples and do not exclude bats as potential viral reservoir in the surveyed geographic area.",
journal = "Tropical Biomedicine",
title = "Failure to detect viral RNA in bat samples collected in the Balkan region",
number = "4",
volume = "33",
pages = "780-785",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2547"
}

Ćirković, V., Stamenković, G., Jovanović, J., Šiljić, M., Paunović, M.,& Stanojević, M.. (2016). Failure to detect viral RNA in bat samples collected in the Balkan region. in Tropical Biomedicine, 33(4), 780-785.
https://hdl.handle.net/21.15107/rcub_ibiss_2547

Ćirković V, Stamenković G, Jovanović J, Šiljić M, Paunović M, Stanojević M. Failure to detect viral RNA in bat samples collected in the Balkan region. in Tropical Biomedicine. 2016;33(4):780-785.
https://hdl.handle.net/21.15107/rcub_ibiss_2547 .

Ćirković, Valentina, Stamenković, Gorana, Jovanović, Jelena, Šiljić, Marina, Paunović, Milan, Stanojević, Maja, "Failure to detect viral RNA in bat samples collected in the Balkan region" in Tropical Biomedicine, 33, no. 4 (2016):780-785,
https://hdl.handle.net/21.15107/rcub_ibiss_2547 .

TY  - CONF
AU  - Zorić, Katarina
AU  - Marković, Vanja
AU  - Tomović, Jelena
AU  - Atanacković, Ana
AU  - Vasiljević, Božica
AU  - Čanak Atlagić, Jelena
AU  - Anđus, Stefan
AU  - Kračun-Kolarević, Margareta
AU  - Marinković, Nikola
AU  - Popović, Nataša
AU  - Raković, Maja
AU  - Đuknić, Jelena
AU  - Đikanović, Vesna
AU  - Tubić, Bojana
AU  - Ilić, Marija
AU  - Paunović, Momir
AU  - Nikolić, Vera
AU  - Stamenković, Gorana
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5674
AB  - Specimens from three populations of native neritid snail Theodoxus danubialis (C. Pfeiffer, 1828) from the Balkan (the Una, Lepenica and Nišava Rivers) were processed. Obtained 16s rRNA sequences, along with additional sequences taken from GenBank were analysed. Maximum likelihood consensus tree showing relations of plotted sequenceswas discussed. Sequences from the Nišava River sample differ from the other T. danubialis sequences, pointing to specific16s rRNA haplotype. In order to better access intraspecific variabilityand diversity of T. danubilis, an use of less conservative markers should be implemented.
PB  - Bor: Technical Faculty
C3  - Proceedings: XXIV International Conference: Ecological Truth: Eco-Ist'16; 2016 Jun 12-15; Vrnjačka Banja, Serbia
T1  - Towards assessing genetic diversity of Theodoxus danubialis (C. Pfeiffer, 1828) (Gastropoda; Neritidae) from Central Balkan
SP  - 61
EP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5674
ER  - 

@conference{
author = "Zorić, Katarina and Marković, Vanja and Tomović, Jelena and Atanacković, Ana and Vasiljević, Božica and Čanak Atlagić, Jelena and Anđus, Stefan and Kračun-Kolarević, Margareta and Marinković, Nikola and Popović, Nataša and Raković, Maja and Đuknić, Jelena and Đikanović, Vesna and Tubić, Bojana and Ilić, Marija and Paunović, Momir and Nikolić, Vera and Stamenković, Gorana",
year = "2016",
abstract = "Specimens from three populations of native neritid snail Theodoxus danubialis (C. Pfeiffer, 1828) from the Balkan (the Una, Lepenica and Nišava Rivers) were processed. Obtained 16s rRNA sequences, along with additional sequences taken from GenBank were analysed. Maximum likelihood consensus tree showing relations of plotted sequenceswas discussed. Sequences from the Nišava River sample differ from the other T. danubialis sequences, pointing to specific16s rRNA haplotype. In order to better access intraspecific variabilityand diversity of T. danubilis, an use of less conservative markers should be implemented.",
publisher = "Bor: Technical Faculty",
journal = "Proceedings: XXIV International Conference: Ecological Truth: Eco-Ist'16; 2016 Jun 12-15; Vrnjačka Banja, Serbia",
title = "Towards assessing genetic diversity of Theodoxus danubialis (C. Pfeiffer, 1828) (Gastropoda; Neritidae) from Central Balkan",
pages = "61-65",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5674"
}

Zorić, K., Marković, V., Tomović, J., Atanacković, A., Vasiljević, B., Čanak Atlagić, J., Anđus, S., Kračun-Kolarević, M., Marinković, N., Popović, N., Raković, M., Đuknić, J., Đikanović, V., Tubić, B., Ilić, M., Paunović, M., Nikolić, V.,& Stamenković, G.. (2016). Towards assessing genetic diversity of Theodoxus danubialis (C. Pfeiffer, 1828) (Gastropoda; Neritidae) from Central Balkan. in Proceedings: XXIV International Conference: Ecological Truth: Eco-Ist'16; 2016 Jun 12-15; Vrnjačka Banja, Serbia
Bor: Technical Faculty., 61-65.
https://hdl.handle.net/21.15107/rcub_ibiss_5674

Zorić K, Marković V, Tomović J, Atanacković A, Vasiljević B, Čanak Atlagić J, Anđus S, Kračun-Kolarević M, Marinković N, Popović N, Raković M, Đuknić J, Đikanović V, Tubić B, Ilić M, Paunović M, Nikolić V, Stamenković G. Towards assessing genetic diversity of Theodoxus danubialis (C. Pfeiffer, 1828) (Gastropoda; Neritidae) from Central Balkan. in Proceedings: XXIV International Conference: Ecological Truth: Eco-Ist'16; 2016 Jun 12-15; Vrnjačka Banja, Serbia. 2016;:61-65.
https://hdl.handle.net/21.15107/rcub_ibiss_5674 .

Zorić, Katarina, Marković, Vanja, Tomović, Jelena, Atanacković, Ana, Vasiljević, Božica, Čanak Atlagić, Jelena, Anđus, Stefan, Kračun-Kolarević, Margareta, Marinković, Nikola, Popović, Nataša, Raković, Maja, Đuknić, Jelena, Đikanović, Vesna, Tubić, Bojana, Ilić, Marija, Paunović, Momir, Nikolić, Vera, Stamenković, Gorana, "Towards assessing genetic diversity of Theodoxus danubialis (C. Pfeiffer, 1828) (Gastropoda; Neritidae) from Central Balkan" in Proceedings: XXIV International Conference: Ecological Truth: Eco-Ist'16; 2016 Jun 12-15; Vrnjačka Banja, Serbia (2016):61-65,
https://hdl.handle.net/21.15107/rcub_ibiss_5674 .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Blagojević, Jelena
AU  - Liehr, Thomas
AU  - Kosyakova, Nadezda
AU  - Rajičić, Marija
AU  - Vujošević, Mladen
PY  - 2016
UR  - http://dx.plos.org/10.1371/journal.pone.0160946
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2524
AB  - Since the density of simple sequence repeats (SSRs) may vary between different chromosomes of the same species in eukaryotic genomes, we screened SSRs of the whole genome of the yellow necked mouse, Apodemus flavicollis, in order to reveal SSR profiles specific for animals carrying B chromosomes. We found that the 2200 bp band was amplified by primer (CAG)4AC to a highly increased level in samples with B chromosomes. This quantitative difference (B-marker) between animals with (+B) and without (0B) B chromosomes was used to screen 20 populations (387 animals). The presence/absence of Bs was confirmed in 96.5% of 342 non mosaic individuals, which recommends this method for noninvasive B-presence detection. A group of 45 animals with mosaic and micro B (μB) karyotypes was considered separately and showed 55.6% of overall congruence between karyotyping and molecular screening results. Relative quantification by qPCR of two different targeted sequences from B-marker indicated that these B-specific fragments are multiplied on B chromosomes. It also confirms our assumption that different types of Bs with variable molecular composition may exist in the same individual and between individuals of this species. Our results substantiate the origin of Bs from the standard chromosomal complement. The B-marker showed 98% sequence identity with the serine/threonine protein kinase VRK1 gene, similarly to findings reported for Bs from phylogenetically highly distant mammalian species. Evolutionarily conserved protein-coding genes found in Bs, including this one in A. flavicollis, could suggest a common evolutionary pathway.
PB  - Public Library of Science
T2  - PLOS ONE
T1  - Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis
IS  - 8
VL  - 11
DO  - 10.1371/journal.pone.0160946
SP  - e0160946
EP  - e0160946
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Blagojević, Jelena and Liehr, Thomas and Kosyakova, Nadezda and Rajičić, Marija and Vujošević, Mladen",
year = "2016",
abstract = "Since the density of simple sequence repeats (SSRs) may vary between different chromosomes of the same species in eukaryotic genomes, we screened SSRs of the whole genome of the yellow necked mouse, Apodemus flavicollis, in order to reveal SSR profiles specific for animals carrying B chromosomes. We found that the 2200 bp band was amplified by primer (CAG)4AC to a highly increased level in samples with B chromosomes. This quantitative difference (B-marker) between animals with (+B) and without (0B) B chromosomes was used to screen 20 populations (387 animals). The presence/absence of Bs was confirmed in 96.5% of 342 non mosaic individuals, which recommends this method for noninvasive B-presence detection. A group of 45 animals with mosaic and micro B (μB) karyotypes was considered separately and showed 55.6% of overall congruence between karyotyping and molecular screening results. Relative quantification by qPCR of two different targeted sequences from B-marker indicated that these B-specific fragments are multiplied on B chromosomes. It also confirms our assumption that different types of Bs with variable molecular composition may exist in the same individual and between individuals of this species. Our results substantiate the origin of Bs from the standard chromosomal complement. The B-marker showed 98% sequence identity with the serine/threonine protein kinase VRK1 gene, similarly to findings reported for Bs from phylogenetically highly distant mammalian species. Evolutionarily conserved protein-coding genes found in Bs, including this one in A. flavicollis, could suggest a common evolutionary pathway.",
publisher = "Public Library of Science",
journal = "PLOS ONE",
title = "Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis",
number = "8",
volume = "11",
doi = "10.1371/journal.pone.0160946",
pages = "e0160946-e0160946"
}

Bugarski-Stanojević, V., Stamenković, G., Blagojević, J., Liehr, T., Kosyakova, N., Rajičić, M.,& Vujošević, M.. (2016). Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis. in PLOS ONE
Public Library of Science., 11(8), e0160946-e0160946.
https://doi.org/10.1371/journal.pone.0160946

Bugarski-Stanojević V, Stamenković G, Blagojević J, Liehr T, Kosyakova N, Rajičić M, Vujošević M. Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis. in PLOS ONE. 2016;11(8):e0160946-e0160946.
doi:10.1371/journal.pone.0160946 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Blagojević, Jelena, Liehr, Thomas, Kosyakova, Nadezda, Rajičić, Marija, Vujošević, Mladen, "Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis" in PLOS ONE, 11, no. 8 (2016):e0160946-e0160946,
https://doi.org/10.1371/journal.pone.0160946 . .

TY  - JOUR
AU  - Ilić, Marija
AU  - Stamenković, Gorana
AU  - Nikolić, Valentina
AU  - Marković, Vanja
AU  - Marinković, Nikola
AU  - Paunović, Momir
AU  - Crnobrnja-Isailović, Jelka
PY  - 2016
UR  - http://epa.oszk.hu/02500/02583/00043/pdf/EPA02583_applied_ecology_2016_02_381397.pdf
UR  - http://www.aloki.hu/pdf/1402_381397.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2596
AB  - Many European frogs and toads are relatively secretive species and except during breeding season, adults can rarely be seen during time-restricted fieldwork. In contrast, their tadpoles are easy to record and could be very useful in a brief biodiversity assessment. It is important to perform quick and accurate taxonomic identification of tadpoles, yet genetic methods are costly and cannot be routinely applied. We tested suitability of morphometric analysis for taxonomical distinction among tadpoles of early breeding local anuran species. Tadpole samples were collected simultaneously at three different locations in Republic of Serbia (South-eastern Europe) in habitats known to be breeding sites shared by brown frogs and the common toad. DNA barcoding verified Rana dalmatina, R. temporaria and Bufo bufo species, each collected in different location. The results of linear morphometric analyses suggested that relative head length and head width could be good discriminative characteristics for tadpoles of these two Rana species and those of B. bufo. To distinguish between tadpoles of two analyzed brown frog species, relative tail length could be used. For further development of the identification procedures for tadpoles of particular species,
T2  - Applied Ecology and Environmental Research
T1  - Identification of syntopic anuran species in early tadpole stages: correspondence between morphometric and genetic data
IS  - 2
VL  - 14
DO  - 10.15666/aeer/1402_381397
SP  - 381
EP  - 397
ER  - 

@article{
author = "Ilić, Marija and Stamenković, Gorana and Nikolić, Valentina and Marković, Vanja and Marinković, Nikola and Paunović, Momir and Crnobrnja-Isailović, Jelka",
year = "2016",
abstract = "Many European frogs and toads are relatively secretive species and except during breeding season, adults can rarely be seen during time-restricted fieldwork. In contrast, their tadpoles are easy to record and could be very useful in a brief biodiversity assessment. It is important to perform quick and accurate taxonomic identification of tadpoles, yet genetic methods are costly and cannot be routinely applied. We tested suitability of morphometric analysis for taxonomical distinction among tadpoles of early breeding local anuran species. Tadpole samples were collected simultaneously at three different locations in Republic of Serbia (South-eastern Europe) in habitats known to be breeding sites shared by brown frogs and the common toad. DNA barcoding verified Rana dalmatina, R. temporaria and Bufo bufo species, each collected in different location. The results of linear morphometric analyses suggested that relative head length and head width could be good discriminative characteristics for tadpoles of these two Rana species and those of B. bufo. To distinguish between tadpoles of two analyzed brown frog species, relative tail length could be used. For further development of the identification procedures for tadpoles of particular species,",
journal = "Applied Ecology and Environmental Research",
title = "Identification of syntopic anuran species in early tadpole stages: correspondence between morphometric and genetic data",
number = "2",
volume = "14",
doi = "10.15666/aeer/1402_381397",
pages = "381-397"
}

Ilić, M., Stamenković, G., Nikolić, V., Marković, V., Marinković, N., Paunović, M.,& Crnobrnja-Isailović, J.. (2016). Identification of syntopic anuran species in early tadpole stages: correspondence between morphometric and genetic data. in Applied Ecology and Environmental Research, 14(2), 381-397.
https://doi.org/10.15666/aeer/1402_381397

Ilić M, Stamenković G, Nikolić V, Marković V, Marinković N, Paunović M, Crnobrnja-Isailović J. Identification of syntopic anuran species in early tadpole stages: correspondence between morphometric and genetic data. in Applied Ecology and Environmental Research. 2016;14(2):381-397.
doi:10.15666/aeer/1402_381397 .

Ilić, Marija, Stamenković, Gorana, Nikolić, Valentina, Marković, Vanja, Marinković, Nikola, Paunović, Momir, Crnobrnja-Isailović, Jelka, "Identification of syntopic anuran species in early tadpole stages: correspondence between morphometric and genetic data" in Applied Ecology and Environmental Research, 14, no. 2 (2016):381-397,
https://doi.org/10.15666/aeer/1402_381397 . .

TY  - GEN
AU  - Đurović, Jelena
AU  - Stojković, Oliver
AU  - Todorović, Jelena
AU  - Brajić, Aleksandra
AU  - Stanković, Sanja
AU  - Obradović, Svetlana
AU  - Stamenković, Gorana
PY  - 2016
UR  - https://www.tandfonline.com/doi/full/10.1080/14647273.2016.1255785
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2595
AB  - Reproductive failure (recurrent foetal loss, unexplained infertility and IVF implantation failure) may be, in a number of cases, explained by thrombophilia, either acquired or inherited. Several genes contribute to thrombophilia, some with major effect (Factor V, Factor II), and some with minor effect (MTHFR, PAI-1, ATIII, etc.). The aim of this study was to estimate frequency of thrombophilia-associated genotypes (FII20210G > A, FV1691G > A, MTHFR677C > T and PAI-1 -675 4G/5G) in a group of 1631 Serbian women experiencing reproductive failure, and compare it with a healthy, female control group. Our results showed marginally significant (p = 0.050) differences in allele frequencies between patients and controls for the FV1691 mutations. For the FII20210G > A, although the statistical significance was not achieved (p = 0.076), we found higher frequency of variant allele in patients compared to controls (1.87% vs. 0.38%, respectively) which may point to a possible role of this polymorphism in thrombotic events. For the MTHFR677C > T and PAI-1 -675 4G/5G, we found no difference in distributions of genotype or allele frequencies between these two groups (p > 0.05). For three subjects with very rare genotypes (two patients homozygous for FV1691G > A and one patient homozygous for FII20210G > A) we performed additional biochemical analyses for haemostasis, as well as genotyping of two polymorphisms (MTHFR1298A > C and ATIII786G > A).
T2  - Human Fertility
T1  - Genetics of suspected thrombophilia in Serbian females with infertility, including three cases, homozygous for FII 20210A or FV 1691A mutations
DO  - 10.1080/14647273.2016.1255785
SP  - 1
EP  - 8
ER  - 

@misc{
author = "Đurović, Jelena and Stojković, Oliver and Todorović, Jelena and Brajić, Aleksandra and Stanković, Sanja and Obradović, Svetlana and Stamenković, Gorana",
year = "2016",
abstract = "Reproductive failure (recurrent foetal loss, unexplained infertility and IVF implantation failure) may be, in a number of cases, explained by thrombophilia, either acquired or inherited. Several genes contribute to thrombophilia, some with major effect (Factor V, Factor II), and some with minor effect (MTHFR, PAI-1, ATIII, etc.). The aim of this study was to estimate frequency of thrombophilia-associated genotypes (FII20210G > A, FV1691G > A, MTHFR677C > T and PAI-1 -675 4G/5G) in a group of 1631 Serbian women experiencing reproductive failure, and compare it with a healthy, female control group. Our results showed marginally significant (p = 0.050) differences in allele frequencies between patients and controls for the FV1691 mutations. For the FII20210G > A, although the statistical significance was not achieved (p = 0.076), we found higher frequency of variant allele in patients compared to controls (1.87% vs. 0.38%, respectively) which may point to a possible role of this polymorphism in thrombotic events. For the MTHFR677C > T and PAI-1 -675 4G/5G, we found no difference in distributions of genotype or allele frequencies between these two groups (p > 0.05). For three subjects with very rare genotypes (two patients homozygous for FV1691G > A and one patient homozygous for FII20210G > A) we performed additional biochemical analyses for haemostasis, as well as genotyping of two polymorphisms (MTHFR1298A > C and ATIII786G > A).",
journal = "Human Fertility",
title = "Genetics of suspected thrombophilia in Serbian females with infertility, including three cases, homozygous for FII 20210A or FV 1691A mutations",
doi = "10.1080/14647273.2016.1255785",
pages = "1-8"
}

Đurović, J., Stojković, O., Todorović, J., Brajić, A., Stanković, S., Obradović, S.,& Stamenković, G.. (2016). Genetics of suspected thrombophilia in Serbian females with infertility, including three cases, homozygous for FII 20210A or FV 1691A mutations. in Human Fertility, 1-8.
https://doi.org/10.1080/14647273.2016.1255785

Đurović J, Stojković O, Todorović J, Brajić A, Stanković S, Obradović S, Stamenković G. Genetics of suspected thrombophilia in Serbian females with infertility, including three cases, homozygous for FII 20210A or FV 1691A mutations. in Human Fertility. 2016;:1-8.
doi:10.1080/14647273.2016.1255785 .

Đurović, Jelena, Stojković, Oliver, Todorović, Jelena, Brajić, Aleksandra, Stanković, Sanja, Obradović, Svetlana, Stamenković, Gorana, "Genetics of suspected thrombophilia in Serbian females with infertility, including three cases, homozygous for FII 20210A or FV 1691A mutations" in Human Fertility (2016):1-8,
https://doi.org/10.1080/14647273.2016.1255785 . .

TY  - CONF
AU  - Rajičić, Marija
AU  - Adnađević, Tanja
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Jojić, Vida
AU  - Budinski, Ivana
AU  - Jovanović, Vladimir
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
PY  - 2015
UR  - http://chromosome2015.mcb.nsc.ru/chr_2015_abstracts.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5423
AB  - In genus Apodemus, the presence of B chromosome has been confirmed in six out of
twenty-two species. One of them is yellow-necked field mouse Apodemus flavicollis. The
presence of B chromosomes has been confirmed in almost all studied populations of A. flavicollis
46
in Serbia in a wide range of frequencies (0,07- 0,63). Five B chromosomes per animal was the
highest number of Bs recorded in our analyzed populations.
These extra chromosomes in A. flavicollis are euchromatic and acrocentric, and by size
and distribution of G- and C-bands can be sorted into the group of five smallest chromosomes in
karyotype.
The absence of Bs accumulation in male meiosis, no significant difference in the mean
number of scars and embryos between females with and without Bs, as well as the absence of
significant difference in the presence of Bs among six age categories, speaks in favor of heterotic
model of their maintenance, contrary to the model of parasitic behavior of these additional
genomic elements.
In general, the number of Bs carriers is higher in environment that is not optimal for this
species which indicates that those individuals have higher fitness. Seasonal variation of Bs
frequencies has been recorded, in such a manner that highest frequencies are present before and
after winter, with stable frequency fluctuations during successive years. Different frequency of Bs
carriers is related to adaptive differentiation to diverse habitats mediated by environmental
variables that directly and/or indirectly influence population dynamics of A. flavicollis. Changes
in frequency follow up increase in population density in circumstances without food and space
competition. Positive correlation between the frequency of Bs carriers and the average number of
sub zero days, as well as with altitude increase, has been recorded.
Considering phenotypic effects, higher level of morphological integration of mandible in
animals with Bs in A. flavicollis has been found. Furthermore, studies of the effects of Bs on
nonmetric cranial traits, as well as on three components of cranial variability confirmed that Bs
does not disturb developmental homeostasis in their carriers. Moreover, Bs play a significant role
in structuring cranial variation.
Negative correlation between the expression of Tgf-β gene and the presence of Bs was also
observed. This could be of great importance for B chromosome which has to pass through
different mitotic and meiotic check points, and Tgf-β gene, through cell cycle regulation,
influences development and homeostasis.
The existence of specific DNA profiles and differential expressions of three genes in the
presence of Bs, testify about interaction of Bs with the rest of the genome. Absence of C-KIT
exon 1 in more than one copy in specimens with 1, 2 and 3B chromosomes excludes the
possibility that this gene is present on Bs. On the other hand, presence of S18 gene copy has been
indicated on some Bs.
Considering the results of population studies, euchromatic structure of Bs and molecular
studies that have been conducted so far, we can say that the contribution of Bs to overall genetic
47
diversity in this species is in sight, but there is still need for more specific evidence such as
precise determination of Bs molecular structure, origin and function.
PB  - Institute of molecular and cellular biology SB RAS
C3  - Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia
T1  - B chromosomes in Apodemus flavicollis
SP  - 45
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5423
ER  - 

@conference{
author = "Rajičić, Marija and Adnađević, Tanja and Bugarski-Stanojević, Vanja and Stamenković, Gorana and Jojić, Vida and Budinski, Ivana and Jovanović, Vladimir and Blagojević, Jelena and Vujošević, Mladen",
year = "2015",
abstract = "In genus Apodemus, the presence of B chromosome has been confirmed in six out of
twenty-two species. One of them is yellow-necked field mouse Apodemus flavicollis. The
presence of B chromosomes has been confirmed in almost all studied populations of A. flavicollis
46
in Serbia in a wide range of frequencies (0,07- 0,63). Five B chromosomes per animal was the
highest number of Bs recorded in our analyzed populations.
These extra chromosomes in A. flavicollis are euchromatic and acrocentric, and by size
and distribution of G- and C-bands can be sorted into the group of five smallest chromosomes in
karyotype.
The absence of Bs accumulation in male meiosis, no significant difference in the mean
number of scars and embryos between females with and without Bs, as well as the absence of
significant difference in the presence of Bs among six age categories, speaks in favor of heterotic
model of their maintenance, contrary to the model of parasitic behavior of these additional
genomic elements.
In general, the number of Bs carriers is higher in environment that is not optimal for this
species which indicates that those individuals have higher fitness. Seasonal variation of Bs
frequencies has been recorded, in such a manner that highest frequencies are present before and
after winter, with stable frequency fluctuations during successive years. Different frequency of Bs
carriers is related to adaptive differentiation to diverse habitats mediated by environmental
variables that directly and/or indirectly influence population dynamics of A. flavicollis. Changes
in frequency follow up increase in population density in circumstances without food and space
competition. Positive correlation between the frequency of Bs carriers and the average number of
sub zero days, as well as with altitude increase, has been recorded.
Considering phenotypic effects, higher level of morphological integration of mandible in
animals with Bs in A. flavicollis has been found. Furthermore, studies of the effects of Bs on
nonmetric cranial traits, as well as on three components of cranial variability confirmed that Bs
does not disturb developmental homeostasis in their carriers. Moreover, Bs play a significant role
in structuring cranial variation.
Negative correlation between the expression of Tgf-β gene and the presence of Bs was also
observed. This could be of great importance for B chromosome which has to pass through
different mitotic and meiotic check points, and Tgf-β gene, through cell cycle regulation,
influences development and homeostasis.
The existence of specific DNA profiles and differential expressions of three genes in the
presence of Bs, testify about interaction of Bs with the rest of the genome. Absence of C-KIT
exon 1 in more than one copy in specimens with 1, 2 and 3B chromosomes excludes the
possibility that this gene is present on Bs. On the other hand, presence of S18 gene copy has been
indicated on some Bs.
Considering the results of population studies, euchromatic structure of Bs and molecular
studies that have been conducted so far, we can say that the contribution of Bs to overall genetic
47
diversity in this species is in sight, but there is still need for more specific evidence such as
precise determination of Bs molecular structure, origin and function.",
publisher = "Institute of molecular and cellular biology SB RAS",
journal = "Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia",
title = "B chromosomes in Apodemus flavicollis",
pages = "45-47",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5423"
}

Rajičić, M., Adnađević, T., Bugarski-Stanojević, V., Stamenković, G., Jojić, V., Budinski, I., Jovanović, V., Blagojević, J.,& Vujošević, M.. (2015). B chromosomes in Apodemus flavicollis. in Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia
Institute of molecular and cellular biology SB RAS., 45-47.
https://hdl.handle.net/21.15107/rcub_ibiss_5423

Rajičić M, Adnađević T, Bugarski-Stanojević V, Stamenković G, Jojić V, Budinski I, Jovanović V, Blagojević J, Vujošević M. B chromosomes in Apodemus flavicollis. in Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia. 2015;:45-47.
https://hdl.handle.net/21.15107/rcub_ibiss_5423 .

Rajičić, Marija, Adnađević, Tanja, Bugarski-Stanojević, Vanja, Stamenković, Gorana, Jojić, Vida, Budinski, Ivana, Jovanović, Vladimir, Blagojević, Jelena, Vujošević, Mladen, "B chromosomes in Apodemus flavicollis" in Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia (2015):45-47,
https://hdl.handle.net/21.15107/rcub_ibiss_5423 .