TY  - JOUR
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6553
AB  - Introduction: In the present study, we assessed the effects of the hyper- harmonized- 
hydroxylated fullerene– water complex (3HFWC) on Alzheimer's disease (AD) neuro
pathological hallmarks in 5XFAD mice, an AD animal model.
 Methods: The 3- week- old 5XFAD mice were exposed to 3HFWC water solution ad li
bitum for 3 months in the presymptomatic phase of pathology. The functional effects 
of the treatment were confirmed through near- infrared spectroscopy (NIRS) analysis 
through machine learning (ML) using artificial neural networks (ANNs) to classify the 
control and 3HFWC- treated brain tissue samples. The effects of 3HFWC treatment 
on amyloid- β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in 
cortical and hippocampal tissue were assessed.
 Results: The 3HFWC treatment significantly decreased the amyloid- β plaque load in 
specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not 
induce the activation of glia (astrocytes and microglia) nor did it negatively affect 
synaptic protein markers (GAP- 43, synaptophysin, and PSD- 95).
 Conclusion: The obtained results point to the potential of 3HFWC, when applied in 
the presymptomatic phase of AD, to interfere with amyloid plaque formation without 
inducing AD- related pathological processes such as neuroinflammation, gliosis, and 
synaptic vulnerability.
PB  - Wiley
T2  - CNS Neuroscience and Therapeutics
T1  - The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease
IS  - 3
VL  - 30
DO  - 10.1111/cns.14188
SP  - e14188
ER  - 

@article{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2024",
abstract = "Introduction: In the present study, we assessed the effects of the hyper- harmonized- 
hydroxylated fullerene– water complex (3HFWC) on Alzheimer's disease (AD) neuro
pathological hallmarks in 5XFAD mice, an AD animal model.
 Methods: The 3- week- old 5XFAD mice were exposed to 3HFWC water solution ad li
bitum for 3 months in the presymptomatic phase of pathology. The functional effects 
of the treatment were confirmed through near- infrared spectroscopy (NIRS) analysis 
through machine learning (ML) using artificial neural networks (ANNs) to classify the 
control and 3HFWC- treated brain tissue samples. The effects of 3HFWC treatment 
on amyloid- β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in 
cortical and hippocampal tissue were assessed.
 Results: The 3HFWC treatment significantly decreased the amyloid- β plaque load in 
specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not 
induce the activation of glia (astrocytes and microglia) nor did it negatively affect 
synaptic protein markers (GAP- 43, synaptophysin, and PSD- 95).
 Conclusion: The obtained results point to the potential of 3HFWC, when applied in 
the presymptomatic phase of AD, to interfere with amyloid plaque formation without 
inducing AD- related pathological processes such as neuroinflammation, gliosis, and 
synaptic vulnerability.",
publisher = "Wiley",
journal = "CNS Neuroscience and Therapeutics",
title = "The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease",
number = "3",
volume = "30",
doi = "10.1111/cns.14188",
pages = "e14188"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2024). The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease. in CNS Neuroscience and Therapeutics
Wiley., 30(3), e14188.
https://doi.org/10.1111/cns.14188

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease. in CNS Neuroscience and Therapeutics. 2024;30(3):e14188.
doi:10.1111/cns.14188 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease" in CNS Neuroscience and Therapeutics, 30, no. 3 (2024):e14188,
https://doi.org/10.1111/cns.14188 . .

TY  - JOUR
AU  - Jovanović Macura, Irena
AU  - Živanović, Ana
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Major, Tamara
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6548
AB  - Cerebral amyloid angiopathy (CAA) is characterized by amyloid (A ) accumulation in the blood vessels and is associated with cognitive impairment in Alzheimer’s disease (AD). The increased accumulation of A is also present in the retinal blood vessels and a significant correlation between retinal and brain amyloid deposition was demonstrated in living patients and animal AD models. The A accumulation in the retinal blood vessels can be the result of impaired transcytosis and/or the dysfunctional ocular glymphatic system in AD and during aging. We analyzed the changes in the mRNA and protein expression of major facilitator superfamily domain-containing protein2a (Mfsd2a), the major regulator of transcytosis, and of Aquaporin4 (Aqp4), the key player implicated in the functioning of the glymphatic system, in the retinas of 4- and 12-month-old WT and 5xFAD female mice. A strong decrease in the Mfsd2a mRNA and protein expression was observed in the 4 Mand12M5xFADand12MWTretinas. Theincrease in the expression of srebp1-c could be at least partially responsible for the Mfsd2a decrease in the 4 M 5xFAD retinas. The decrease in the pericyte (CD13+) coverage of retinal blood vessels in the 4 M and 12 M 5xFAD retinas and in the 12 MWTretinas suggests that pericyte loss could be associated with the Mfsd2a downregulation in these experimental groups. The observed increase in Aqp4 expression in 4 M and 12 M 5xFAD and 12 M WT retinas accompanied by the decreased perivascular Aqp4 expression is indicative of the impaired glymphatic system. The findings in this study reveal the impaired Mfsd2a and Aqp4 expression and Aqp4 perivascular mislocalization in retinal blood vessels during physiolog ical (WT) and pathological (5xFAD) aging, indicating their importance as putative targets for the development of new treatments that can improve the regulation of transcytosis or the function of the glymphatic system.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease
IS  - 18
VL  - 24
DO  - 10.3390/ijms241814092
SP  - 14092
ER  - 

@article{
author = "Jovanović Macura, Irena and Živanović, Ana and Perović, Milka and Ćirić, Jelena and Major, Tamara and Kanazir, Selma and Ivković, Sanja",
year = "2023",
abstract = "Cerebral amyloid angiopathy (CAA) is characterized by amyloid (A ) accumulation in the blood vessels and is associated with cognitive impairment in Alzheimer’s disease (AD). The increased accumulation of A is also present in the retinal blood vessels and a significant correlation between retinal and brain amyloid deposition was demonstrated in living patients and animal AD models. The A accumulation in the retinal blood vessels can be the result of impaired transcytosis and/or the dysfunctional ocular glymphatic system in AD and during aging. We analyzed the changes in the mRNA and protein expression of major facilitator superfamily domain-containing protein2a (Mfsd2a), the major regulator of transcytosis, and of Aquaporin4 (Aqp4), the key player implicated in the functioning of the glymphatic system, in the retinas of 4- and 12-month-old WT and 5xFAD female mice. A strong decrease in the Mfsd2a mRNA and protein expression was observed in the 4 Mand12M5xFADand12MWTretinas. Theincrease in the expression of srebp1-c could be at least partially responsible for the Mfsd2a decrease in the 4 M 5xFAD retinas. The decrease in the pericyte (CD13+) coverage of retinal blood vessels in the 4 M and 12 M 5xFAD retinas and in the 12 MWTretinas suggests that pericyte loss could be associated with the Mfsd2a downregulation in these experimental groups. The observed increase in Aqp4 expression in 4 M and 12 M 5xFAD and 12 M WT retinas accompanied by the decreased perivascular Aqp4 expression is indicative of the impaired glymphatic system. The findings in this study reveal the impaired Mfsd2a and Aqp4 expression and Aqp4 perivascular mislocalization in retinal blood vessels during physiolog ical (WT) and pathological (5xFAD) aging, indicating their importance as putative targets for the development of new treatments that can improve the regulation of transcytosis or the function of the glymphatic system.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease",
number = "18",
volume = "24",
doi = "10.3390/ijms241814092",
pages = "14092"
}

Jovanović Macura, I., Živanović, A., Perović, M., Ćirić, J., Major, T., Kanazir, S.,& Ivković, S.. (2023). The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease. in International Journal of Molecular Sciences
Basel: MDPI., 24(18), 14092.
https://doi.org/10.3390/ijms241814092

Jovanović Macura I, Živanović A, Perović M, Ćirić J, Major T, Kanazir S, Ivković S. The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease. in International Journal of Molecular Sciences. 2023;24(18):14092.
doi:10.3390/ijms241814092 .

Jovanović Macura, Irena, Živanović, Ana, Perović, Milka, Ćirić, Jelena, Major, Tamara, Kanazir, Selma, Ivković, Sanja, "The Expression of Major Facilitator Superfamily  Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is  Altered in the Retinas of a 5xFAD Mouse Model of  Alzheimer’s Disease" in International Journal of Molecular Sciences, 24, no. 18 (2023):14092,
https://doi.org/10.3390/ijms241814092 . .

TY  - CONF
AU  - Jovanović Macura, Irena
AU  - Đuričić, Ivana
AU  - Major, Tamara
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Sobajić, Slađana
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5858
AB  - Mfsd2a is expressed mainly in the endothelial cells and is an essential regulator of 
blood vessel transcytosis. Therefore, decrease in Mfsd2a expression can be a risk 
factor for developing leaky blood vessels. Mfsd2a is also the main docosahexaenoic 
acid (DHA, C22:6n3) transporter. DHA, an omega-3 fatty acid, is one of the main 
structural lipids of the neuronal and vascular retina, crucial for the normal functioning 
of photoreceptors (PRs). However, the capacity of the retina to synthesize DHA is 
limited, and the maintenance of retinal DHA content relies on the uptake from blood borne lipids. The currently recommended FO doses yielded low PUFAs tissue 
bioavailability, and supplementation with higher doses has been increasingly 
recommended. Nevertheless, the effects of higher FO doses on retinal Mfsd2a 
expression and blood vessels coverage are unknown.
Western blot and qPCR analyses showed that high dose FO supplementation increased 
Mfsd2a expression in the retina. Immunohistochemical analyses of Mfsd2a expression 
on retinal blood vessels (labeled with 488-conjugated Lycopersicon esculentum, 
lectin) and subsequent ImageJ analyses revealed 1.32-fold increase in the Mfsd2a 
retinal blood vessel coverage. In the same time the pericyte blood vessel coverage 
(CD13+ cells) was not affected with FO supplementation, and the increase in Mfsd2a 
blood vessel expression is not the result of the increased pericyte coverage. 
Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of 
the retinal blood vessels that can serve either as prophylaxis in the healthy eye or as an 
adjuvant in developing targeted manipulations of the barrier during diseases.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice
SP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5858
ER  - 

@conference{
author = "Jovanović Macura, Irena and Đuričić, Ivana and Major, Tamara and Milanović, Desanka and Brkić, Marjana and Sobajić, Slađana and Kanazir, Selma and Ivković, Sanja",
year = "2023",
abstract = "Mfsd2a is expressed mainly in the endothelial cells and is an essential regulator of 
blood vessel transcytosis. Therefore, decrease in Mfsd2a expression can be a risk 
factor for developing leaky blood vessels. Mfsd2a is also the main docosahexaenoic 
acid (DHA, C22:6n3) transporter. DHA, an omega-3 fatty acid, is one of the main 
structural lipids of the neuronal and vascular retina, crucial for the normal functioning 
of photoreceptors (PRs). However, the capacity of the retina to synthesize DHA is 
limited, and the maintenance of retinal DHA content relies on the uptake from blood borne lipids. The currently recommended FO doses yielded low PUFAs tissue 
bioavailability, and supplementation with higher doses has been increasingly 
recommended. Nevertheless, the effects of higher FO doses on retinal Mfsd2a 
expression and blood vessels coverage are unknown.
Western blot and qPCR analyses showed that high dose FO supplementation increased 
Mfsd2a expression in the retina. Immunohistochemical analyses of Mfsd2a expression 
on retinal blood vessels (labeled with 488-conjugated Lycopersicon esculentum, 
lectin) and subsequent ImageJ analyses revealed 1.32-fold increase in the Mfsd2a 
retinal blood vessel coverage. In the same time the pericyte blood vessel coverage 
(CD13+ cells) was not affected with FO supplementation, and the increase in Mfsd2a 
blood vessel expression is not the result of the increased pericyte coverage. 
Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of 
the retinal blood vessels that can serve either as prophylaxis in the healthy eye or as an 
adjuvant in developing targeted manipulations of the barrier during diseases.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice",
pages = "66",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5858"
}

Jovanović Macura, I., Đuričić, I., Major, T., Milanović, D., Brkić, M., Sobajić, S., Kanazir, S.,& Ivković, S.. (2023). The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 66.
https://hdl.handle.net/21.15107/rcub_ibiss_5858

Jovanović Macura I, Đuričić I, Major T, Milanović D, Brkić M, Sobajić S, Kanazir S, Ivković S. The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:66.
https://hdl.handle.net/21.15107/rcub_ibiss_5858 .

Jovanović Macura, Irena, Đuričić, Ivana, Major, Tamara, Milanović, Desanka, Brkić, Marjana, Sobajić, Slađana, Kanazir, Selma, Ivković, Sanja, "The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):66,
https://hdl.handle.net/21.15107/rcub_ibiss_5858 .

TY  - JOUR
AU  - Jovanović Macura, Irena
AU  - Đuričić, Ivana
AU  - Major, Tamara
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Šobajić, Slađana
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1756464622003723
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5174
AB  - The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels.
T2  - Journal of Functional Foods
T1  - The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice
VL  - 99
DO  - 10.1016/j.jff.2022.105302
SP  - 105302
ER  - 

@article{
author = "Jovanović Macura, Irena and Đuričić, Ivana and Major, Tamara and Milanović, Desanka and Brkić, Marjana and Šobajić, Slađana and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels.",
journal = "Journal of Functional Foods",
title = "The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice",
volume = "99",
doi = "10.1016/j.jff.2022.105302",
pages = "105302"
}

Jovanović Macura, I., Đuričić, I., Major, T., Milanović, D., Brkić, M., Šobajić, S., Kanazir, S.,& Ivković, S.. (2022). The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice. in Journal of Functional Foods, 99, 105302.
https://doi.org/10.1016/j.jff.2022.105302

Jovanović Macura I, Đuričić I, Major T, Milanović D, Brkić M, Šobajić S, Kanazir S, Ivković S. The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice. in Journal of Functional Foods. 2022;99:105302.
doi:10.1016/j.jff.2022.105302 .

Jovanović Macura, Irena, Đuričić, Ivana, Major, Tamara, Milanović, Desanka, Brkić, Marjana, Šobajić, Slađana, Kanazir, Selma, Ivković, Sanja, "The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice" in Journal of Functional Foods, 99 (2022):105302,
https://doi.org/10.1016/j.jff.2022.105302 . .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Ivković, Sanja
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9599456
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5172
AB  - Glucocorticoids are the most potent anti-inflammatory agents known. Limited in vivo data are available to characterize the mechanism underlying their cognitive side effects and transient occurrence of steroid psychosis. Cholesterol is important for proper neurotransmission and brain plasticity, and disruption of its homeostasis in the brain has been closely associated with memory decline during aging and in age-related neurodegenerative disorders. In the present study, we assessed the direct effects of dexamethasone, a potent synthetic glucocorticoid, on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46A1), major enzymes involved in cholesterol synthesis, metabolism, and excretion, respectively. The effects of the dexamethasone were examined during aging, in the cortex and hippocampus of 6-, 12- and 18-month-old rats, and following long-term food restriction (FR). The most prominent change observed was the age-related decrease in ApoE mRNA regardless of the food regimen applied. In animals kept on FR, this decrease was accompanied by an increase in the mRNA expression of HMGCR and CYP46A1. The present study also demonstrates that food restriction reversed most of the dexamethasone-induced changes in the expression of genes involved in regulation of cholesterol homeostasis in aging rats, in a region-specific manner.
PB  - Basel: MDPI
T2  - Brain Sciences
T1  - Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging
IS  - 10
VL  - 12
DO  - 10.3390/brainsci12101297
SP  - 1297
ER  - 

@article{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Ivković, Sanja and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Glucocorticoids are the most potent anti-inflammatory agents known. Limited in vivo data are available to characterize the mechanism underlying their cognitive side effects and transient occurrence of steroid psychosis. Cholesterol is important for proper neurotransmission and brain plasticity, and disruption of its homeostasis in the brain has been closely associated with memory decline during aging and in age-related neurodegenerative disorders. In the present study, we assessed the direct effects of dexamethasone, a potent synthetic glucocorticoid, on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46A1), major enzymes involved in cholesterol synthesis, metabolism, and excretion, respectively. The effects of the dexamethasone were examined during aging, in the cortex and hippocampus of 6-, 12- and 18-month-old rats, and following long-term food restriction (FR). The most prominent change observed was the age-related decrease in ApoE mRNA regardless of the food regimen applied. In animals kept on FR, this decrease was accompanied by an increase in the mRNA expression of HMGCR and CYP46A1. The present study also demonstrates that food restriction reversed most of the dexamethasone-induced changes in the expression of genes involved in regulation of cholesterol homeostasis in aging rats, in a region-specific manner.",
publisher = "Basel: MDPI",
journal = "Brain Sciences",
title = "Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging",
number = "10",
volume = "12",
doi = "10.3390/brainsci12101297",
pages = "1297"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Ivković, S., Kanazir, S.,& Perović, M.. (2022). Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging. in Brain Sciences
Basel: MDPI., 12(10), 1297.
https://doi.org/10.3390/brainsci12101297

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Ivković S, Kanazir S, Perović M. Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging. in Brain Sciences. 2022;12(10):1297.
doi:10.3390/brainsci12101297 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Ivković, Sanja, Kanazir, Selma, Perović, Milka, "Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging" in Brain Sciences, 12, no. 10 (2022):1297,
https://doi.org/10.3390/brainsci12101297 . .

TY  - JOUR
AU  - Ivković, Sanja
AU  - Major, Tamara
AU  - Mitić, Miloš
AU  - Lončarević-Vasiljković, Nataša
AU  - Jović, Milena
AU  - Adžić, Miroslav
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0024320522001709
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35283177
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4942
AB  - The brain is the softest organ in the body, and any change in the mechanical properties of the tissue induces the activation of glial cells, astrocytes and microglia. Amyloid plaques, one of the main pathological features of Alzheimer's disease (AD), are substantially harder than the surrounding brain tissue and can activate astrocytes and microglia resulting in the glial engulfment of plaques. Durotaxis, a migratory preference towards stiffer tissue, is prompting microglia to form a mechanical barrier around plaques reducing amyloid β (Aβ) induced neurotoxicity. Mechanoreceptors are highly expressed in the brain, particularly in microglia. The large increase in the expression of the mechanoreceptor Piezo1 was observed in the brains from AD animal models and AD patients in plaque encompassing glia. Importantly, Piezo1 function is regulated via force-from-lipids through the lipid composition of the membrane and membranous incorporation of polyunsaturated fatty acids (PUFAs) can affect the function of Piezo1 altering mechanosensitive properties of the cell. On the other hand, PUFAs dietary supplementation can alter microglial polarization, the envelopment of amyloid plaques, and immune response and Piezo1 activity was implicated in the similar modulations of microglia behavior. Finally, PUFAs treatment is currently in use in medical trials as the therapy for sickle cell anemia, a disease linked with the mutations in Piezo1. Further studies are needed to elucidate the connection between PUFAs, Piezo1 expression, and microglia behavior in the AD brain. These findings could open new possibilities in harnessing microglia in AD and in developing novel therapeutic strategies.
T2  - Life sciences
T1  - Fatty acids as biomodulators of Piezo1 mediated glial mechanosensitivity in Alzheimer's disease.
VL  - 297
DO  - 10.1016/j.lfs.2022.120470
SP  - 120470
ER  - 

@article{
author = "Ivković, Sanja and Major, Tamara and Mitić, Miloš and Lončarević-Vasiljković, Nataša and Jović, Milena and Adžić, Miroslav",
year = "2022",
abstract = "The brain is the softest organ in the body, and any change in the mechanical properties of the tissue induces the activation of glial cells, astrocytes and microglia. Amyloid plaques, one of the main pathological features of Alzheimer's disease (AD), are substantially harder than the surrounding brain tissue and can activate astrocytes and microglia resulting in the glial engulfment of plaques. Durotaxis, a migratory preference towards stiffer tissue, is prompting microglia to form a mechanical barrier around plaques reducing amyloid β (Aβ) induced neurotoxicity. Mechanoreceptors are highly expressed in the brain, particularly in microglia. The large increase in the expression of the mechanoreceptor Piezo1 was observed in the brains from AD animal models and AD patients in plaque encompassing glia. Importantly, Piezo1 function is regulated via force-from-lipids through the lipid composition of the membrane and membranous incorporation of polyunsaturated fatty acids (PUFAs) can affect the function of Piezo1 altering mechanosensitive properties of the cell. On the other hand, PUFAs dietary supplementation can alter microglial polarization, the envelopment of amyloid plaques, and immune response and Piezo1 activity was implicated in the similar modulations of microglia behavior. Finally, PUFAs treatment is currently in use in medical trials as the therapy for sickle cell anemia, a disease linked with the mutations in Piezo1. Further studies are needed to elucidate the connection between PUFAs, Piezo1 expression, and microglia behavior in the AD brain. These findings could open new possibilities in harnessing microglia in AD and in developing novel therapeutic strategies.",
journal = "Life sciences",
title = "Fatty acids as biomodulators of Piezo1 mediated glial mechanosensitivity in Alzheimer's disease.",
volume = "297",
doi = "10.1016/j.lfs.2022.120470",
pages = "120470"
}

Ivković, S., Major, T., Mitić, M., Lončarević-Vasiljković, N., Jović, M.,& Adžić, M.. (2022). Fatty acids as biomodulators of Piezo1 mediated glial mechanosensitivity in Alzheimer's disease.. in Life sciences, 297, 120470.
https://doi.org/10.1016/j.lfs.2022.120470

Ivković S, Major T, Mitić M, Lončarević-Vasiljković N, Jović M, Adžić M. Fatty acids as biomodulators of Piezo1 mediated glial mechanosensitivity in Alzheimer's disease.. in Life sciences. 2022;297:120470.
doi:10.1016/j.lfs.2022.120470 .

Ivković, Sanja, Major, Tamara, Mitić, Miloš, Lončarević-Vasiljković, Nataša, Jović, Milena, Adžić, Miroslav, "Fatty acids as biomodulators of Piezo1 mediated glial mechanosensitivity in Alzheimer's disease." in Life sciences, 297 (2022):120470,
https://doi.org/10.1016/j.lfs.2022.120470 . .

TY  - CONF
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5615
AB  - Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење и 
најчешћи узрок деменције код старих особа, са преваленцом два пута већом у 
женској популацији.  Упркос дугогодишњим истраживањима механизама 
патогенезе АБ, као и бројним спроведеним претклиничким студијама, још увек не 
постоји адекватна терапија за ово обољење. У овој иницијалној студији испитиван 
је неуропротективни потенцијал нано квантне супстанце 3HFWC  –  хипер-
хармонизованог  комплекса  хидроксилованог фулерена и воде, у  животињском 
моделу АБ  –  трансгеним  5XFAD мишевима. Женке 5XFAD мишева  су  излагане 
нано квантној супстанци 3HFWC у продромалној фази патологије. Третман је 
започет када су животиње биле старе 4 недеље и животиње су појене раствором 
нано квантне  супстанце 3HFWC  уместо воде током наредна три месеца.  Након 
третмана, анализирани су број и морфолошке карактеристике амилоидних плакова 
у структурама мозга од значаја за процесе учења и памћења – кори великог мозга и 
хипокампусу. Испитиван је и ефекат третмана на акумулацију токсичног протеина 
амилоида бета (Аβ), као и промене у маркерима синаптичке пластичности. Третман 
са 3HFWC је значајно смањио заступљеност амилоидних плакова у одређеним 
регионима коре великог мозга. Резултати стога указују  на неуропротективно 
дејство превентивне примене  нано  квантне  супстанце 3HFWC  у  мишјем моделу 
Алцхајмерове болести.
AB  - Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje i najčešći uzrok demencije kod starih osoba, sa prevalencom dva puta većom u ženskoj populaciji. Uprkos dugogodišnjim istraživanjima mehanizama patogeneze AB, kao i brojnim sprovedenim pretkliničkim studijama, još uvek ne postoji adekvatna terapija za ovo oboljenje. U ovoj inicijalnoj studiji ispitivan je neuroprotektivni potencijal nano kvantne supstance 3HFWC – hiper- harmonizovanog kompleksa hidroksilovanog fulerena i vode, u životinjskom modelu AB – transgenim 5XFAD miševima. Ženke 5XFAD miševa su izlagane nano kvantnoj supstanci 3HFWC u prodromalnoj fazi patologije. Tretman je započet kada su životinje bile stare 4 nedelje i životinje su pojene rastvorom nano kvantne supstance 3HFWC umesto vode tokom naredna tri meseca. Nakon tretmana, analizirani su broj i morfološke karakteristike amiloidnih plakova u strukturama mozga od značaja za procese učenja i pamćenja – kori velikog mozga i hipokampusu. Ispitivan je i efekat tretmana na akumulaciju toksičnog proteina amiloida beta (Aβ), kao i promene u markerima sinaptičke plastičnosti. Tretman sa 3HFWC je značajno smanjio zastupljenost amiloidnih plakova u određenim regionima kore velikog mozga. Rezultati stoga ukazuju na neuroprotektivno dejstvo preventivne primene nano kvantne supstance 3HFWC u mišjem modelu Alchajmerove bolesti.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti
T1  - Испитивање неуропротективног потенцијала наноквантне супстанце 3HFWC у мишијем моделу Алцхајмерове болести
SP  - 317
SP  - M64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5615
ER  - 

@conference{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење и 
најчешћи узрок деменције код старих особа, са преваленцом два пута већом у 
женској популацији.  Упркос дугогодишњим истраживањима механизама 
патогенезе АБ, као и бројним спроведеним претклиничким студијама, још увек не 
постоји адекватна терапија за ово обољење. У овој иницијалној студији испитиван 
је неуропротективни потенцијал нано квантне супстанце 3HFWC  –  хипер-
хармонизованог  комплекса  хидроксилованог фулерена и воде, у  животињском 
моделу АБ  –  трансгеним  5XFAD мишевима. Женке 5XFAD мишева  су  излагане 
нано квантној супстанци 3HFWC у продромалној фази патологије. Третман је 
започет када су животиње биле старе 4 недеље и животиње су појене раствором 
нано квантне  супстанце 3HFWC  уместо воде током наредна три месеца.  Након 
третмана, анализирани су број и морфолошке карактеристике амилоидних плакова 
у структурама мозга од значаја за процесе учења и памћења – кори великог мозга и 
хипокампусу. Испитиван је и ефекат третмана на акумулацију токсичног протеина 
амилоида бета (Аβ), као и промене у маркерима синаптичке пластичности. Третман 
са 3HFWC је значајно смањио заступљеност амилоидних плакова у одређеним 
регионима коре великог мозга. Резултати стога указују  на неуропротективно 
дејство превентивне примене  нано  квантне  супстанце 3HFWC  у  мишјем моделу 
Алцхајмерове болести., Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje i najčešći uzrok demencije kod starih osoba, sa prevalencom dva puta većom u ženskoj populaciji. Uprkos dugogodišnjim istraživanjima mehanizama patogeneze AB, kao i brojnim sprovedenim pretkliničkim studijama, još uvek ne postoji adekvatna terapija za ovo oboljenje. U ovoj inicijalnoj studiji ispitivan je neuroprotektivni potencijal nano kvantne supstance 3HFWC – hiper- harmonizovanog kompleksa hidroksilovanog fulerena i vode, u životinjskom modelu AB – transgenim 5XFAD miševima. Ženke 5XFAD miševa su izlagane nano kvantnoj supstanci 3HFWC u prodromalnoj fazi patologije. Tretman je započet kada su životinje bile stare 4 nedelje i životinje su pojene rastvorom nano kvantne supstance 3HFWC umesto vode tokom naredna tri meseca. Nakon tretmana, analizirani su broj i morfološke karakteristike amiloidnih plakova u strukturama mozga od značaja za procese učenja i pamćenja – kori velikog mozga i hipokampusu. Ispitivan je i efekat tretmana na akumulaciju toksičnog proteina amiloida beta (Aβ), kao i promene u markerima sinaptičke plastičnosti. Tretman sa 3HFWC je značajno smanjio zastupljenost amiloidnih plakova u određenim regionima kore velikog mozga. Rezultati stoga ukazuju na neuroprotektivno dejstvo preventivne primene nano kvantne supstance 3HFWC u mišjem modelu Alchajmerove bolesti.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti, Испитивање неуропротективног потенцијала наноквантне супстанце 3HFWC у мишијем моделу Алцхајмерове болести",
pages = "317-M64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5615"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2022). Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 317.
https://hdl.handle.net/21.15107/rcub_ibiss_5615

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:317.
https://hdl.handle.net/21.15107/rcub_ibiss_5615 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):317,
https://hdl.handle.net/21.15107/rcub_ibiss_5615 .

TY  - CONF
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5188
AB  - The potential of fullerenes and fullerene’s water-soluble derivatives
to bind to amyloid-b has been well documented in vitro and
in silico. However, the anti-amyloid action of fullerenols in
in vivo treatments has not been fully examined. In the present study we assessed the effects of the hydroxylated fullerene-water
complex (3HFWC) on Alzheimer’s disease (AD) neuropathological
hallmarks in 5XFAD mice, a well-recognized AD animal
model. The 3-week-old 5XFAD mice were exposed to 3HFWC
water solution ad libitum for 3 months. The 3HFWC treatment
started in the presymptomatic phase of pathology and analyses
were focused on the effects on amyloid-b (Ab) accumulation, plaque
formation, gliosis, and synaptic plasticity in cortical and hippocampal
tissue. The 3HFWC treatment significantly decreased
the amyloid-b plaque load in specific parts of cerebral cortex, followed
by the unchanged levels of Ab. None of these changes
were detected in the hippocampus. At the same time, 3HFWC
treatment did not exacerbate the activation of glial cells, nor
altered the expression levels of synaptic proteins. The obtained
results point to the potential of 3HFWC, when applied in the
presymptomatic phase of AD, to interfere with Ab accumulation
and amyloid plaque formation without exacerbating the other
AD-related pathological processes.
PB  - Hoboken : John Wiley & Sons Ltd
C3  - The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
T1  - Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease
DO  - 10.1002/2211-5463.13440
SP  - 130
ER  - 

@conference{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "The potential of fullerenes and fullerene’s water-soluble derivatives
to bind to amyloid-b has been well documented in vitro and
in silico. However, the anti-amyloid action of fullerenols in
in vivo treatments has not been fully examined. In the present study we assessed the effects of the hydroxylated fullerene-water
complex (3HFWC) on Alzheimer’s disease (AD) neuropathological
hallmarks in 5XFAD mice, a well-recognized AD animal
model. The 3-week-old 5XFAD mice were exposed to 3HFWC
water solution ad libitum for 3 months. The 3HFWC treatment
started in the presymptomatic phase of pathology and analyses
were focused on the effects on amyloid-b (Ab) accumulation, plaque
formation, gliosis, and synaptic plasticity in cortical and hippocampal
tissue. The 3HFWC treatment significantly decreased
the amyloid-b plaque load in specific parts of cerebral cortex, followed
by the unchanged levels of Ab. None of these changes
were detected in the hippocampus. At the same time, 3HFWC
treatment did not exacerbate the activation of glial cells, nor
altered the expression levels of synaptic proteins. The obtained
results point to the potential of 3HFWC, when applied in the
presymptomatic phase of AD, to interfere with Ab accumulation
and amyloid plaque formation without exacerbating the other
AD-related pathological processes.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal",
title = "Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease",
doi = "10.1002/2211-5463.13440",
pages = "130"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2022). Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
Hoboken : John Wiley & Sons Ltd., 130.
https://doi.org/10.1002/2211-5463.13440

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal. 2022;:130.
doi:10.1002/2211-5463.13440 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease" in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal (2022):130,
https://doi.org/10.1002/2211-5463.13440 . .

TY  - CONF
AU  - Jović, Milena
AU  - Lončarević-Vasiljković, Nataša
AU  - Ivković, Sanja
AU  - Milanović, Desanka
AU  - Kanazir, Selma
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5803
AB  - Aims: Alzheimer’s disease (AD) is the most prevalent type of dementia in elderly,triggered by multiple factors such as amyloid 
plaques, neurofibrillary tangles and neuroinflammation. The use of supplements with omega-3 fatty acids has been associated 
with reduced risk and lessened AD pathology. The purpose of this study was to elucidate the mechanisms underlying effects of 
short-term fish oil (FO) suplementation in presymptomatic stage of AD in 5xFAD mice.
Methods: Three-month old female 5xFAD mice received FO (100μl/animal/day) via oral gavage during the period of 3 weeks. 
Western blot and immunohistochemical analysis were used to detect changes in pathological features of AD in cortex of 5xFAD 
mice. AmiloGlo was used to visualize plaques. Amyloid beta (Aβ) peptide, dystrophic neurites (DNs), microglia/macrophages 
and CX3CR1/CX3CL1 were detect by anti-Aβ42-, anti-SMI31-, p-Tau-, anti-Iba-1-, anti-CX3CR1 anti-CX3CL1 antibodies, 
retrospectively. Immunostaining was observed by confocal microscopy. Quantification was done by Image J and Image Quant 
softwer. 
Results: The present study shows that short-term FO supplementation applied in presymptomatic stage of AD, alters the 
behaviour of microglia/macrophages prompting them to establish a physical barrier around amyloid plaques. This barrier 
significantly suppresses DNs formation through the reduction of both Aβ content and tau hyperphosphorylation. Moreover, the 
short-term FO treatment suppresses CX3CR1/CX3CL1 axis, interaction between microglial cells and neurons, which represents 
one of possible mechanisms for altered microglial/macrophage motility and colocalization around plaques.
Conclusion: Our findings suggest that FO consumption may play an important role in modulating microglial response and 
ameliorating the AD pathology in presymptomatic stage of Alzheimer’s disease.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Mechanism underlying effects of fish oil supplementation in presymptomatic stage of Alzheimer disease in 5XFAD mice
SP  - 287
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5803
ER  - 

@conference{
author = "Jović, Milena and Lončarević-Vasiljković, Nataša and Ivković, Sanja and Milanović, Desanka and Kanazir, Selma",
year = "2019",
abstract = "Aims: Alzheimer’s disease (AD) is the most prevalent type of dementia in elderly,triggered by multiple factors such as amyloid 
plaques, neurofibrillary tangles and neuroinflammation. The use of supplements with omega-3 fatty acids has been associated 
with reduced risk and lessened AD pathology. The purpose of this study was to elucidate the mechanisms underlying effects of 
short-term fish oil (FO) suplementation in presymptomatic stage of AD in 5xFAD mice.
Methods: Three-month old female 5xFAD mice received FO (100μl/animal/day) via oral gavage during the period of 3 weeks. 
Western blot and immunohistochemical analysis were used to detect changes in pathological features of AD in cortex of 5xFAD 
mice. AmiloGlo was used to visualize plaques. Amyloid beta (Aβ) peptide, dystrophic neurites (DNs), microglia/macrophages 
and CX3CR1/CX3CL1 were detect by anti-Aβ42-, anti-SMI31-, p-Tau-, anti-Iba-1-, anti-CX3CR1 anti-CX3CL1 antibodies, 
retrospectively. Immunostaining was observed by confocal microscopy. Quantification was done by Image J and Image Quant 
softwer. 
Results: The present study shows that short-term FO supplementation applied in presymptomatic stage of AD, alters the 
behaviour of microglia/macrophages prompting them to establish a physical barrier around amyloid plaques. This barrier 
significantly suppresses DNs formation through the reduction of both Aβ content and tau hyperphosphorylation. Moreover, the 
short-term FO treatment suppresses CX3CR1/CX3CL1 axis, interaction between microglial cells and neurons, which represents 
one of possible mechanisms for altered microglial/macrophage motility and colocalization around plaques.
Conclusion: Our findings suggest that FO consumption may play an important role in modulating microglial response and 
ameliorating the AD pathology in presymptomatic stage of Alzheimer’s disease.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Mechanism underlying effects of fish oil supplementation in presymptomatic stage of Alzheimer disease in 5XFAD mice",
pages = "287",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5803"
}

Jović, M., Lončarević-Vasiljković, N., Ivković, S., Milanović, D.,& Kanazir, S.. (2019). Mechanism underlying effects of fish oil supplementation in presymptomatic stage of Alzheimer disease in 5XFAD mice. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 287.
https://hdl.handle.net/21.15107/rcub_ibiss_5803

Jović M, Lončarević-Vasiljković N, Ivković S, Milanović D, Kanazir S. Mechanism underlying effects of fish oil supplementation in presymptomatic stage of Alzheimer disease in 5XFAD mice. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:287.
https://hdl.handle.net/21.15107/rcub_ibiss_5803 .

Jović, Milena, Lončarević-Vasiljković, Nataša, Ivković, Sanja, Milanović, Desanka, Kanazir, Selma, "Mechanism underlying effects of fish oil supplementation in presymptomatic stage of Alzheimer disease in 5XFAD mice" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):287,
https://hdl.handle.net/21.15107/rcub_ibiss_5803 .

TY  - JOUR
AU  - Ivković, Sanja
AU  - Jovanović Macura, Irena
AU  - Antonijević, Tijana
AU  - Kanazir, Selma
AU  - Henrique, Domingos
PY  - 2019
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4389
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3648
AB  - Epidermal growth factor (EGF) signaling has been implicated in the regulation of the differentiation and proliferation of retinal progenitors. We assessed how different levels of EGF signaling, achieved either by increasing receptor expression or via addition of the exogenous ligand, or an increase in both, can affect the differentiation of progenitors in the first week of postnatal retinal development in the model system of retinal explants (REs). Proliferating progenitor cells in REs were infected with either the control CLV3/ESR-related peptide family (CLE)-green fluorescent protein (GFP)-or with EGF receptor (EGFR)-GFP-expressing retrovirus, and grown in the control medium or in the presence of exogenous EGF (10 ng/mL). The differentiation of infected cells into Muller glia (Sox9+), rod photoreceptors (rhodopsin+) and horizontal cells (calbindin+) was analyzed. In all the examined conditions, infected cells differentiated into Muller glia and rod photoreceptors that normally develop postnatally. Horizontal cells finished their development during the embryonic stages and progenitors infected with control-GFP virus did not differentiate into GFP+/calbindin-in either control or EGFsupplemented medium, however, cells infected with EGFR-GFP differentiated into horizontal cells (GFP+/calbindin+) in both culture conditions. These results imply that altering the levels of EGFR and/or the amount of the EGF ligand can overcome progenitor competence restriction.
T2  - Archives of Biological Sciences
T1  - Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina
IS  - 4
VL  - 71
DO  - 10.2298/abs190617054i
SP  - 711
EP  - 719
ER  - 

@article{
author = "Ivković, Sanja and Jovanović Macura, Irena and Antonijević, Tijana and Kanazir, Selma and Henrique, Domingos",
year = "2019",
abstract = "Epidermal growth factor (EGF) signaling has been implicated in the regulation of the differentiation and proliferation of retinal progenitors. We assessed how different levels of EGF signaling, achieved either by increasing receptor expression or via addition of the exogenous ligand, or an increase in both, can affect the differentiation of progenitors in the first week of postnatal retinal development in the model system of retinal explants (REs). Proliferating progenitor cells in REs were infected with either the control CLV3/ESR-related peptide family (CLE)-green fluorescent protein (GFP)-or with EGF receptor (EGFR)-GFP-expressing retrovirus, and grown in the control medium or in the presence of exogenous EGF (10 ng/mL). The differentiation of infected cells into Muller glia (Sox9+), rod photoreceptors (rhodopsin+) and horizontal cells (calbindin+) was analyzed. In all the examined conditions, infected cells differentiated into Muller glia and rod photoreceptors that normally develop postnatally. Horizontal cells finished their development during the embryonic stages and progenitors infected with control-GFP virus did not differentiate into GFP+/calbindin-in either control or EGFsupplemented medium, however, cells infected with EGFR-GFP differentiated into horizontal cells (GFP+/calbindin+) in both culture conditions. These results imply that altering the levels of EGFR and/or the amount of the EGF ligand can overcome progenitor competence restriction.",
journal = "Archives of Biological Sciences",
title = "Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina",
number = "4",
volume = "71",
doi = "10.2298/abs190617054i",
pages = "711-719"
}

Ivković, S., Jovanović Macura, I., Antonijević, T., Kanazir, S.,& Henrique, D.. (2019). Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina. in Archives of Biological Sciences, 71(4), 711-719.
https://doi.org/10.2298/abs190617054i

Ivković S, Jovanović Macura I, Antonijević T, Kanazir S, Henrique D. Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina. in Archives of Biological Sciences. 2019;71(4):711-719.
doi:10.2298/abs190617054i .

Ivković, Sanja, Jovanović Macura, Irena, Antonijević, Tijana, Kanazir, Selma, Henrique, Domingos, "Different levels of epidermal growth factor signaling modifies the differentiation of specific cell types in mouse postnatal retina" in Archives of Biological Sciences, 71, no. 4 (2019):711-719,
https://doi.org/10.2298/abs190617054i . .

TY  - JOUR
AU  - Jović, Milena
AU  - Lončarević-Vasiljković, Nataša
AU  - Ivković, Sanja
AU  - Dinić, Jelena
AU  - Milanović, Desanka
AU  - Zloković, Berislav
AU  - Kanazir, Selma
PY  - 2019
UR  - http://dx.plos.org/10.1371/journal.pone.0216726
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6522015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3405
AB  - Dystrophic neurites and activated microglia are one of the main neuropathological characteristics of Alzheimer's disease (AD). Although the use of supplements with omega-3 fatty acids has been associated with reduced risk and lessened AD pathology, it still remains elusive whether such a treatment could affect dystrophic neurites (DNs) formation and microglia/macrophage behavior in the early phase of disease. We analyzed the effects of short-term (3 weeks) fish oil supplementation on DNs formation, tau hyperphosphorylation, Amyloid-beta peptide 1-42 (Aβ42) levels and microglial/macrophage response to AD pathology in the parietal cortex of 4-month-old 5xFAD mice, a mouse model of AD. The present study shows for the first time that short-term FO supplementation applied in presymptomatic stage of AD, alters the behaviour of microglia/macrophages prompting them to establish a physical barrier around amyloid plaques. This barrier significantly suppresses DNs formation through the reduction of both Aβ content and tau hyperphosphorylation. Moreover, the short-term FO treatment neither suppresses inflammation nor enhances phagocytic properties of microglia/macrophages in the response to Aβ pathology, the effects most commonly attributed to the fish oil supplementation. Our findings suggest that fish oil consumption may play an important role in modulating microglial/macrophage response and ameliorating the AD pathology in presymptomatic stage of Alzheimer's disease.
T2  - PloS One
T1  - Short-term fish oil supplementation applied in presymptomatic stage of Alzheimer's disease enhances microglial/macrophage barrier and prevents neuritic dystrophy in parietal cortex of 5xFAD mouse model.
IS  - 5
VL  - 14
DO  - 10.1371/journal.pone.0216726
SP  - e0216726
ER  - 

@article{
author = "Jović, Milena and Lončarević-Vasiljković, Nataša and Ivković, Sanja and Dinić, Jelena and Milanović, Desanka and Zloković, Berislav and Kanazir, Selma",
year = "2019",
abstract = "Dystrophic neurites and activated microglia are one of the main neuropathological characteristics of Alzheimer's disease (AD). Although the use of supplements with omega-3 fatty acids has been associated with reduced risk and lessened AD pathology, it still remains elusive whether such a treatment could affect dystrophic neurites (DNs) formation and microglia/macrophage behavior in the early phase of disease. We analyzed the effects of short-term (3 weeks) fish oil supplementation on DNs formation, tau hyperphosphorylation, Amyloid-beta peptide 1-42 (Aβ42) levels and microglial/macrophage response to AD pathology in the parietal cortex of 4-month-old 5xFAD mice, a mouse model of AD. The present study shows for the first time that short-term FO supplementation applied in presymptomatic stage of AD, alters the behaviour of microglia/macrophages prompting them to establish a physical barrier around amyloid plaques. This barrier significantly suppresses DNs formation through the reduction of both Aβ content and tau hyperphosphorylation. Moreover, the short-term FO treatment neither suppresses inflammation nor enhances phagocytic properties of microglia/macrophages in the response to Aβ pathology, the effects most commonly attributed to the fish oil supplementation. Our findings suggest that fish oil consumption may play an important role in modulating microglial/macrophage response and ameliorating the AD pathology in presymptomatic stage of Alzheimer's disease.",
journal = "PloS One",
title = "Short-term fish oil supplementation applied in presymptomatic stage of Alzheimer's disease enhances microglial/macrophage barrier and prevents neuritic dystrophy in parietal cortex of 5xFAD mouse model.",
number = "5",
volume = "14",
doi = "10.1371/journal.pone.0216726",
pages = "e0216726"
}

Jović, M., Lončarević-Vasiljković, N., Ivković, S., Dinić, J., Milanović, D., Zloković, B.,& Kanazir, S.. (2019). Short-term fish oil supplementation applied in presymptomatic stage of Alzheimer's disease enhances microglial/macrophage barrier and prevents neuritic dystrophy in parietal cortex of 5xFAD mouse model.. in PloS One, 14(5), e0216726.
https://doi.org/10.1371/journal.pone.0216726

Jović M, Lončarević-Vasiljković N, Ivković S, Dinić J, Milanović D, Zloković B, Kanazir S. Short-term fish oil supplementation applied in presymptomatic stage of Alzheimer's disease enhances microglial/macrophage barrier and prevents neuritic dystrophy in parietal cortex of 5xFAD mouse model.. in PloS One. 2019;14(5):e0216726.
doi:10.1371/journal.pone.0216726 .

Jović, Milena, Lončarević-Vasiljković, Nataša, Ivković, Sanja, Dinić, Jelena, Milanović, Desanka, Zloković, Berislav, Kanazir, Selma, "Short-term fish oil supplementation applied in presymptomatic stage of Alzheimer's disease enhances microglial/macrophage barrier and prevents neuritic dystrophy in parietal cortex of 5xFAD mouse model." in PloS One, 14, no. 5 (2019):e0216726,
https://doi.org/10.1371/journal.pone.0216726 . .

TY  - CONF
AU  - Jović, Milena
AU  - Lončarević-Vasiljković, Nataša
AU  - Ivković, Sanja
AU  - Milanović, Desanka
AU  - Avramović, Vladimir
AU  - Kanazir, Selma
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5719
AB  - Defining features of Alzheimer's disease (AD) pathology are the formation of amyloid plaques, neurofibrillary tangles, neuron loss and inflammation. Plaques are encircled by a halo of diffuse Aβ, surrounded by dystrophic neurites (DNs) and activated glia. High level of Aβ suppresses microglial ability to clear Aβ and activate inflammatory response that becomes neurotoxic. These microglial cells become dysfunctional and can further contribute to AD pathology. 

We investigated the influence of omega-3 fatty acids, the main compounds of fish oil (FO), on Aβ load, neuritic dystrophy and behavior of microglial cells in parietal cortex in 5xFAD mice. 

Three-month old female 5xFAD mice received FO (100μl/animal/day) via oral gavage during 3 weeks period. Aβ-pathology was visualized immunohistochemically. We used ThioflavinS and AmiloGlo to visualize plaques, anti-Aβ42 antibody for soluble Aβ peptide labeling, anti-SMI31 antibody for neuritic dystrophy and anti-Iba-1 antibody for microglial cells. Immunostaining was observed by confocal microscopy. Quantification was done by Image J program. 

Our results showed that short-term FO supplementation was capable of: (i) inducing significant decreased of total Aβ levels (ii) preventing the emergence of neuritic dystrophy in parietal cortex of 5xFAD mice; (iii) increasing overall microglial number; and (iv) enhancing clustering of microglial cells around amyloid plaques, representing mechanical barrier that prevents further Aβ  aggregation. 

This study represents valuable contribution to better biological understanding how FO suppresses AD pathology through typical pleiotropic effect. We believe that FO in combination with other drugs could be good approach for long-term treatment in slowing down AD pathology.
PB  - Brussels, Belgium: Federation of European Neurocience Societies
C3  - 11th FENS Forum of Neuroscience; 2018 Jul 7-11; Berlin, Germany
T1  - Influence of fish oil treatment on microglial cell behavior and Aβ-like pathology in 5xFAD mice model of Alzheimer’s disease
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5719
ER  - 

@conference{
author = "Jović, Milena and Lončarević-Vasiljković, Nataša and Ivković, Sanja and Milanović, Desanka and Avramović, Vladimir and Kanazir, Selma",
year = "2018",
abstract = "Defining features of Alzheimer's disease (AD) pathology are the formation of amyloid plaques, neurofibrillary tangles, neuron loss and inflammation. Plaques are encircled by a halo of diffuse Aβ, surrounded by dystrophic neurites (DNs) and activated glia. High level of Aβ suppresses microglial ability to clear Aβ and activate inflammatory response that becomes neurotoxic. These microglial cells become dysfunctional and can further contribute to AD pathology. 

We investigated the influence of omega-3 fatty acids, the main compounds of fish oil (FO), on Aβ load, neuritic dystrophy and behavior of microglial cells in parietal cortex in 5xFAD mice. 

Three-month old female 5xFAD mice received FO (100μl/animal/day) via oral gavage during 3 weeks period. Aβ-pathology was visualized immunohistochemically. We used ThioflavinS and AmiloGlo to visualize plaques, anti-Aβ42 antibody for soluble Aβ peptide labeling, anti-SMI31 antibody for neuritic dystrophy and anti-Iba-1 antibody for microglial cells. Immunostaining was observed by confocal microscopy. Quantification was done by Image J program. 

Our results showed that short-term FO supplementation was capable of: (i) inducing significant decreased of total Aβ levels (ii) preventing the emergence of neuritic dystrophy in parietal cortex of 5xFAD mice; (iii) increasing overall microglial number; and (iv) enhancing clustering of microglial cells around amyloid plaques, representing mechanical barrier that prevents further Aβ  aggregation. 

This study represents valuable contribution to better biological understanding how FO suppresses AD pathology through typical pleiotropic effect. We believe that FO in combination with other drugs could be good approach for long-term treatment in slowing down AD pathology.",
publisher = "Brussels, Belgium: Federation of European Neurocience Societies",
journal = "11th FENS Forum of Neuroscience; 2018 Jul 7-11; Berlin, Germany",
title = "Influence of fish oil treatment on microglial cell behavior and Aβ-like pathology in 5xFAD mice model of Alzheimer’s disease",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5719"
}

Jović, M., Lončarević-Vasiljković, N., Ivković, S., Milanović, D., Avramović, V.,& Kanazir, S.. (2018). Influence of fish oil treatment on microglial cell behavior and Aβ-like pathology in 5xFAD mice model of Alzheimer’s disease. in 11th FENS Forum of Neuroscience; 2018 Jul 7-11; Berlin, Germany
Brussels, Belgium: Federation of European Neurocience Societies..
https://hdl.handle.net/21.15107/rcub_ibiss_5719

Jović M, Lončarević-Vasiljković N, Ivković S, Milanović D, Avramović V, Kanazir S. Influence of fish oil treatment on microglial cell behavior and Aβ-like pathology in 5xFAD mice model of Alzheimer’s disease. in 11th FENS Forum of Neuroscience; 2018 Jul 7-11; Berlin, Germany. 2018;.
https://hdl.handle.net/21.15107/rcub_ibiss_5719 .

Jović, Milena, Lončarević-Vasiljković, Nataša, Ivković, Sanja, Milanović, Desanka, Avramović, Vladimir, Kanazir, Selma, "Influence of fish oil treatment on microglial cell behavior and Aβ-like pathology in 5xFAD mice model of Alzheimer’s disease" in 11th FENS Forum of Neuroscience; 2018 Jul 7-11; Berlin, Germany (2018),
https://hdl.handle.net/21.15107/rcub_ibiss_5719 .

TY  - CONF
AU  - Jović, Milena
AU  - Lončarević-Vasiljković, Nataša
AU  - Ivković, Sanja
AU  - Milanović, Desanka
AU  - Avramović, Vladimir
AU  - Kanazir, Selma
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5716
AB  - Dystrophic neurites (DNs) and activated microglia are one of the neuropathological characteristics of Alzheimer's disease (AD). Although the use of supplements with omega-3 (0) fatty acids has been associated with reduced risk and lessened. AD pathology, it still remains elusive whether such a treatment could affect DNs formation and microglia behavior in the early phase of disease. We examined influence of fish oil treatment on pathological hallmarks in the brain of 5xFAD mice which rapidly recapitulate major hallmarks of AD amyloid pathology. Three-month old female 5xFAD mice received FO (10011.1/animal/day) via oral Savage during 3 weeks period. Histological analysis was used to detect changes in pathological features of AD in parietal cortex in 5xFAD mice. ThioflavinS and AmiloGlo were used to visualize plaques. Soluble Ap peptide, neuritic dystrophy and microglial cells were detect by anti-A1342-, anti-SMI31- and anti-Iba-1- antibodies, retrospectively. Immunostaining was observed by confocal microscopy. Quantification was done by Image J program. Our results showed that short-term FO supplementation is capable of inducing significant decreased of total Ap levels and preventing the emergence of neuritic dystrophy in parietal cortex of 5xFAD mice. FO supplementation led to increase in overall microglial number and enhanced clustering of microglial cells around amyloid plaques, representing mechanical barrier that doesn't allow AP to aggregate. These results confirmed and extended previous findings suggesting that FO suppresses brain aging and has a typical pleiotropic effect. We believe that FO in combination with other drugs could be good approach for long-term treatment in AD suppression.
PB  - FEBS Balaton 2018
C3  - Final Programme and Book of Abstracts: FEBS3+: From molecules to living systems; 2018 Sep 2-5; Siofok, Hungary
T1  - Influence of fish oil treatment on microglial cell behavior and dystrophic neurites in 5XFAD mice model of Alzheimer's disease
SP  - P8-03
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5716
ER  - 

@conference{
author = "Jović, Milena and Lončarević-Vasiljković, Nataša and Ivković, Sanja and Milanović, Desanka and Avramović, Vladimir and Kanazir, Selma",
year = "2018",
abstract = "Dystrophic neurites (DNs) and activated microglia are one of the neuropathological characteristics of Alzheimer's disease (AD). Although the use of supplements with omega-3 (0) fatty acids has been associated with reduced risk and lessened. AD pathology, it still remains elusive whether such a treatment could affect DNs formation and microglia behavior in the early phase of disease. We examined influence of fish oil treatment on pathological hallmarks in the brain of 5xFAD mice which rapidly recapitulate major hallmarks of AD amyloid pathology. Three-month old female 5xFAD mice received FO (10011.1/animal/day) via oral Savage during 3 weeks period. Histological analysis was used to detect changes in pathological features of AD in parietal cortex in 5xFAD mice. ThioflavinS and AmiloGlo were used to visualize plaques. Soluble Ap peptide, neuritic dystrophy and microglial cells were detect by anti-A1342-, anti-SMI31- and anti-Iba-1- antibodies, retrospectively. Immunostaining was observed by confocal microscopy. Quantification was done by Image J program. Our results showed that short-term FO supplementation is capable of inducing significant decreased of total Ap levels and preventing the emergence of neuritic dystrophy in parietal cortex of 5xFAD mice. FO supplementation led to increase in overall microglial number and enhanced clustering of microglial cells around amyloid plaques, representing mechanical barrier that doesn't allow AP to aggregate. These results confirmed and extended previous findings suggesting that FO suppresses brain aging and has a typical pleiotropic effect. We believe that FO in combination with other drugs could be good approach for long-term treatment in AD suppression.",
publisher = "FEBS Balaton 2018",
journal = "Final Programme and Book of Abstracts: FEBS3+: From molecules to living systems; 2018 Sep 2-5; Siofok, Hungary",
title = "Influence of fish oil treatment on microglial cell behavior and dystrophic neurites in 5XFAD mice model of Alzheimer's disease",
pages = "P8-03",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5716"
}

Jović, M., Lončarević-Vasiljković, N., Ivković, S., Milanović, D., Avramović, V.,& Kanazir, S.. (2018). Influence of fish oil treatment on microglial cell behavior and dystrophic neurites in 5XFAD mice model of Alzheimer's disease. in Final Programme and Book of Abstracts: FEBS3+: From molecules to living systems; 2018 Sep 2-5; Siofok, Hungary
FEBS Balaton 2018., P8-03.
https://hdl.handle.net/21.15107/rcub_ibiss_5716

Jović M, Lončarević-Vasiljković N, Ivković S, Milanović D, Avramović V, Kanazir S. Influence of fish oil treatment on microglial cell behavior and dystrophic neurites in 5XFAD mice model of Alzheimer's disease. in Final Programme and Book of Abstracts: FEBS3+: From molecules to living systems; 2018 Sep 2-5; Siofok, Hungary. 2018;:P8-03.
https://hdl.handle.net/21.15107/rcub_ibiss_5716 .

Jović, Milena, Lončarević-Vasiljković, Nataša, Ivković, Sanja, Milanović, Desanka, Avramović, Vladimir, Kanazir, Selma, "Influence of fish oil treatment on microglial cell behavior and dystrophic neurites in 5XFAD mice model of Alzheimer's disease" in Final Programme and Book of Abstracts: FEBS3+: From molecules to living systems; 2018 Sep 2-5; Siofok, Hungary (2018):P8-03,
https://hdl.handle.net/21.15107/rcub_ibiss_5716 .

TY  - JOUR
AU  - Smiljanić, Kosara
AU  - Todorović, Smilja
AU  - Mladenović, Aleksandra
AU  - Vanmierlo, Tim
AU  - Lütjohann, Dieter
AU  - Ivković, Sanja
AU  - Kanazir, Selma
PY  - 2018
UR  - http://link.springer.com/10.1007/s10522-018-9743-y
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29340834
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2960
AB  - Albeit aging is an inevitable process, the rate of aging is susceptible to modifications. Dietary restriction (DR) is a vigorous nongenetic and nonpharmacological intervention that is known to delay aging and increase healthspan in diverse species. This study aimed to compare the impact of different restricting feeding regimes such as limited daily feeding (LDF, 60% AL) and intermittent feeding (IF) on brain energy homeostasis during aging. The analysis was focused on the key molecules in glucose and cholesterol metabolism in the cortex and hippocampus of middle-aged (12-month-old) and aged (24-month-old) male Wistar rats. We measured the impact of different DRs on the expression levels of AMPK, glucose transporters (GLUT1, GLUT3, GLUT4), and the rate-limiting enzyme in the cholesterol synthesis pathway (HMGCR). Additionally, we assessed the changes in the amounts of cholesterol, its metabolite, and precursors following LDF and IF. IF decreased the levels of AMPK and pAMPK in the cortex while the increased levels were detected in the hippocampus. Glucose metabolism was more affected in the cortex, while cholesterol metabolism was more influenced in the hippocampus. Overall, the hippocampus was more resilient to the DRs, with fewer changes compared to the cortex. We showed that LDF and IF differently affected the brain energy homeostasis during aging and that specific brain regions exhibited distinct vulnerabilities towards DRs. Consequently, special attention should be paid to the DR application among elderly as different phases of aging do not respond equally to altered nutritional regimes.
T2  - Biogerontology
T1  - Limited daily feeding and intermittent feeding have different effects on regional brain energy homeostasis during aging.
IS  - 2
VL  - 19
DO  - 10.1007/s10522-018-9743-y
SP  - 121
EP  - 132
ER  - 

@article{
author = "Smiljanić, Kosara and Todorović, Smilja and Mladenović, Aleksandra and Vanmierlo, Tim and Lütjohann, Dieter and Ivković, Sanja and Kanazir, Selma",
year = "2018",
abstract = "Albeit aging is an inevitable process, the rate of aging is susceptible to modifications. Dietary restriction (DR) is a vigorous nongenetic and nonpharmacological intervention that is known to delay aging and increase healthspan in diverse species. This study aimed to compare the impact of different restricting feeding regimes such as limited daily feeding (LDF, 60% AL) and intermittent feeding (IF) on brain energy homeostasis during aging. The analysis was focused on the key molecules in glucose and cholesterol metabolism in the cortex and hippocampus of middle-aged (12-month-old) and aged (24-month-old) male Wistar rats. We measured the impact of different DRs on the expression levels of AMPK, glucose transporters (GLUT1, GLUT3, GLUT4), and the rate-limiting enzyme in the cholesterol synthesis pathway (HMGCR). Additionally, we assessed the changes in the amounts of cholesterol, its metabolite, and precursors following LDF and IF. IF decreased the levels of AMPK and pAMPK in the cortex while the increased levels were detected in the hippocampus. Glucose metabolism was more affected in the cortex, while cholesterol metabolism was more influenced in the hippocampus. Overall, the hippocampus was more resilient to the DRs, with fewer changes compared to the cortex. We showed that LDF and IF differently affected the brain energy homeostasis during aging and that specific brain regions exhibited distinct vulnerabilities towards DRs. Consequently, special attention should be paid to the DR application among elderly as different phases of aging do not respond equally to altered nutritional regimes.",
journal = "Biogerontology",
title = "Limited daily feeding and intermittent feeding have different effects on regional brain energy homeostasis during aging.",
number = "2",
volume = "19",
doi = "10.1007/s10522-018-9743-y",
pages = "121-132"
}

Smiljanić, K., Todorović, S., Mladenović, A., Vanmierlo, T., Lütjohann, D., Ivković, S.,& Kanazir, S.. (2018). Limited daily feeding and intermittent feeding have different effects on regional brain energy homeostasis during aging.. in Biogerontology, 19(2), 121-132.
https://doi.org/10.1007/s10522-018-9743-y

Smiljanić K, Todorović S, Mladenović A, Vanmierlo T, Lütjohann D, Ivković S, Kanazir S. Limited daily feeding and intermittent feeding have different effects on regional brain energy homeostasis during aging.. in Biogerontology. 2018;19(2):121-132.
doi:10.1007/s10522-018-9743-y .

Smiljanić, Kosara, Todorović, Smilja, Mladenović, Aleksandra, Vanmierlo, Tim, Lütjohann, Dieter, Ivković, Sanja, Kanazir, Selma, "Limited daily feeding and intermittent feeding have different effects on regional brain energy homeostasis during aging." in Biogerontology, 19, no. 2 (2018):121-132,
https://doi.org/10.1007/s10522-018-9743-y . .

TY  - JOUR
AU  - Milanović, Desanka
AU  - Petrovic, Snjezana
AU  - Brkić, Marjana
AU  - Avramović, Vladimir
AU  - Perović, Milka
AU  - Ivković, Sanja
AU  - Glibetić, Marija
AU  - Kanazir, Selma
PY  - 2018
UR  - http://www.mdpi.com/2072-6643/10/9/1250
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3136
AB  - Long-term fish oil (FO) supplementation is able to improve Alzheimer's disease (AD) pathology. We aimed to determine the impact of short-term fish oil (FO) intake on phospholipids composition and plaque pathology in 5xFAD mice, a widely used animal model of AD. A 3-week-long FO supplementation administered at 3 months of age decreased the number of dense core plaques in the 5xFAD cortex and changed phospholipids in the livers and brains of wild-type (Wt) and 5xFAD mice. Livers of both genotypes responded by increase of n-3 and reciprocal decrease of n-6 fatty acids. In Wt brains, FO supplementation induced elevation of n-3 fatty acids and subsequent enhancement of n-6/n-3 ratio. However, in 5xFAD brains the improved n-6/n-3 ratio was mainly due to FO-induced decrease in arachidonic and adrenic n-6 fatty acids. Also, brain and liver abundance of n-3 fatty acids were strongly correlated in Wts, oppositely to 5xFADs where significant brain-liver correlation exists only for n-6 fatty acids. Expression of omega-3 transporter Mfs2a remained unchanged after FO supplementation. We have demonstrated that even a short-term FO intake improves the phospholipid composition and has a significant effect on plaque burden in 5xFAD brains when applied in early stages of AD pathology.
T2  - Nutrients
T1  - Short-Term Fish Oil Treatment Changes the Composition of Phospholipids While Not Affecting the Expression of Mfsd2a Omega-3 Transporter in the Brain and Liver of the 5xFAD Mouse Model of Alzheimer's Disease.
IS  - 9
VL  - 10
DO  - 10.3390/nu10091250
SP  - 1250
ER  - 

@article{
author = "Milanović, Desanka and Petrovic, Snjezana and Brkić, Marjana and Avramović, Vladimir and Perović, Milka and Ivković, Sanja and Glibetić, Marija and Kanazir, Selma",
year = "2018",
abstract = "Long-term fish oil (FO) supplementation is able to improve Alzheimer's disease (AD) pathology. We aimed to determine the impact of short-term fish oil (FO) intake on phospholipids composition and plaque pathology in 5xFAD mice, a widely used animal model of AD. A 3-week-long FO supplementation administered at 3 months of age decreased the number of dense core plaques in the 5xFAD cortex and changed phospholipids in the livers and brains of wild-type (Wt) and 5xFAD mice. Livers of both genotypes responded by increase of n-3 and reciprocal decrease of n-6 fatty acids. In Wt brains, FO supplementation induced elevation of n-3 fatty acids and subsequent enhancement of n-6/n-3 ratio. However, in 5xFAD brains the improved n-6/n-3 ratio was mainly due to FO-induced decrease in arachidonic and adrenic n-6 fatty acids. Also, brain and liver abundance of n-3 fatty acids were strongly correlated in Wts, oppositely to 5xFADs where significant brain-liver correlation exists only for n-6 fatty acids. Expression of omega-3 transporter Mfs2a remained unchanged after FO supplementation. We have demonstrated that even a short-term FO intake improves the phospholipid composition and has a significant effect on plaque burden in 5xFAD brains when applied in early stages of AD pathology.",
journal = "Nutrients",
title = "Short-Term Fish Oil Treatment Changes the Composition of Phospholipids While Not Affecting the Expression of Mfsd2a Omega-3 Transporter in the Brain and Liver of the 5xFAD Mouse Model of Alzheimer's Disease.",
number = "9",
volume = "10",
doi = "10.3390/nu10091250",
pages = "1250"
}

Milanović, D., Petrovic, S., Brkić, M., Avramović, V., Perović, M., Ivković, S., Glibetić, M.,& Kanazir, S.. (2018). Short-Term Fish Oil Treatment Changes the Composition of Phospholipids While Not Affecting the Expression of Mfsd2a Omega-3 Transporter in the Brain and Liver of the 5xFAD Mouse Model of Alzheimer's Disease.. in Nutrients, 10(9), 1250.
https://doi.org/10.3390/nu10091250

Milanović D, Petrovic S, Brkić M, Avramović V, Perović M, Ivković S, Glibetić M, Kanazir S. Short-Term Fish Oil Treatment Changes the Composition of Phospholipids While Not Affecting the Expression of Mfsd2a Omega-3 Transporter in the Brain and Liver of the 5xFAD Mouse Model of Alzheimer's Disease.. in Nutrients. 2018;10(9):1250.
doi:10.3390/nu10091250 .

Milanović, Desanka, Petrovic, Snjezana, Brkić, Marjana, Avramović, Vladimir, Perović, Milka, Ivković, Sanja, Glibetić, Marija, Kanazir, Selma, "Short-Term Fish Oil Treatment Changes the Composition of Phospholipids While Not Affecting the Expression of Mfsd2a Omega-3 Transporter in the Brain and Liver of the 5xFAD Mouse Model of Alzheimer's Disease." in Nutrients, 10, no. 9 (2018):1250,
https://doi.org/10.3390/nu10091250 . .

TY  - CONF
AU  - Lončarević-Vasiljković, Nataša
AU  - Jović, Milena
AU  - Ivković, Sanja
AU  - Milanović, Desanka
AU  - Dinić, Jelena
AU  - Brkić, Marjana
AU  - Avramović, Vladimir
AU  - Kanazir, Selma
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6001
AB  - Introduction. Dystrophic neurites (DNs) are one of the neuropathological characteristics of Alzheimer's disease (AD) and represent the initial phase of neurodegeneration. Microtubule disruption in presynaptic dystrophic neurites that surround plaques impairs axonal transport and leads to the exacerbation of amyloid pathology in AD. Microglia plays a pivotal role in AD pathology as it is able to constitute a physical barrier around amyloid plaques and limit the accumulation of protofibrilar amyloid beta around the fibrillar plaque core. In such a way microglia can mechanically shield the surrounding neurites from the neurotoxic protofibrillar Aβ aggregates. The use of supplements with omega-3 (ω3) fatty acids (FAs), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), such as fish oil, is widespread due to proposed beneficial effects on the nervous system. High DHA consumption has been also associated with reduced risk and lessened AD pathology, yet the mechanisms and therapeutic potential  of these supplements remain elusive. Material and Methods. We analyzed the effects of the short-term fish oil (FO) supplementation on 4 months old 5xFAD mice, a mouse model with fast and robust development of the AD pathology hallmarks such as amyloid plaques and dystrophic neurites. Results. We  showed that even the short treatment with FO can affect the microglia clustering around amyloid plaques and increase the microglial plaque envelopment. Consequently, the Aβ accumulation was reduced and the appearance of DNs substantially suppressed. Conclusion. Our findings suggest that increased DHA consumption may play and important role in modulating microglial response and ameliorating AD pathology at least in the early phase of the disease.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia
T1  - The newly discovered effects of fish oil supplementation on AD pathology: What’s next?
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6001
ER  - 

@conference{
author = "Lončarević-Vasiljković, Nataša and Jović, Milena and Ivković, Sanja and Milanović, Desanka and Dinić, Jelena and Brkić, Marjana and Avramović, Vladimir and Kanazir, Selma",
year = "2017",
abstract = "Introduction. Dystrophic neurites (DNs) are one of the neuropathological characteristics of Alzheimer's disease (AD) and represent the initial phase of neurodegeneration. Microtubule disruption in presynaptic dystrophic neurites that surround plaques impairs axonal transport and leads to the exacerbation of amyloid pathology in AD. Microglia plays a pivotal role in AD pathology as it is able to constitute a physical barrier around amyloid plaques and limit the accumulation of protofibrilar amyloid beta around the fibrillar plaque core. In such a way microglia can mechanically shield the surrounding neurites from the neurotoxic protofibrillar Aβ aggregates. The use of supplements with omega-3 (ω3) fatty acids (FAs), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), such as fish oil, is widespread due to proposed beneficial effects on the nervous system. High DHA consumption has been also associated with reduced risk and lessened AD pathology, yet the mechanisms and therapeutic potential  of these supplements remain elusive. Material and Methods. We analyzed the effects of the short-term fish oil (FO) supplementation on 4 months old 5xFAD mice, a mouse model with fast and robust development of the AD pathology hallmarks such as amyloid plaques and dystrophic neurites. Results. We  showed that even the short treatment with FO can affect the microglia clustering around amyloid plaques and increase the microglial plaque envelopment. Consequently, the Aβ accumulation was reduced and the appearance of DNs substantially suppressed. Conclusion. Our findings suggest that increased DHA consumption may play and important role in modulating microglial response and ameliorating AD pathology at least in the early phase of the disease.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia",
title = "The newly discovered effects of fish oil supplementation on AD pathology: What’s next?",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6001"
}

Lončarević-Vasiljković, N., Jović, M., Ivković, S., Milanović, D., Dinić, J., Brkić, M., Avramović, V.,& Kanazir, S.. (2017). The newly discovered effects of fish oil supplementation on AD pathology: What’s next?. in Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 33.
https://hdl.handle.net/21.15107/rcub_ibiss_6001

Lončarević-Vasiljković N, Jović M, Ivković S, Milanović D, Dinić J, Brkić M, Avramović V, Kanazir S. The newly discovered effects of fish oil supplementation on AD pathology: What’s next?. in Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia. 2017;:33.
https://hdl.handle.net/21.15107/rcub_ibiss_6001 .

Lončarević-Vasiljković, Nataša, Jović, Milena, Ivković, Sanja, Milanović, Desanka, Dinić, Jelena, Brkić, Marjana, Avramović, Vladimir, Kanazir, Selma, "The newly discovered effects of fish oil supplementation on AD pathology: What’s next?" in Book of Abstract: 7th Congress of Serbian Neuroscience Society with international participation; 2017 Oct 25-27; Belgrade, Serbia (2017):33,
https://hdl.handle.net/21.15107/rcub_ibiss_6001 .

TY  - CONF
AU  - Lončarević-Vasiljković, Nataša
AU  - Jović, Milena
AU  - Ivković, Sanja
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Avramović, Vladimir
AU  - Kanazir, Selma
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5715
AB  - Dystrophic neurites (DNs) are one of the neuropathological characteristics of Alzheimer's disease (AD) and represent the initial phase of neurodegeneration. Microtubule disruption in presynaptic dystrophic neurites that surround plaques impairs axonal transport and leads to the exacerbation of amyloid pathology in AD. Microglia plays a pivotal role in AD pathology as it is able to constitute a physical barrier around amyoid plaques and limit the accumulation of protofibrilar amylod beta around the fibrillar plaque core. In such a way microglia can mechanically shield the surrounding neurites from the neurotoxic protofibrillar Ai' aggregates. The use of supplements with omega-3 ( I%03) fatty acids (FAs), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), such as fish oil, is widespread due to proposed beneficial effects on the nervous system, High DHA consumption has been also associated with reduced risk and lessened AD pathology, yet the mechanisms and therapeutic potential of these supplements remain elusive. We analyzed the effects of the short-term fish oil (FO) supplementation on 4 months old 5xFAD mice, a mouse model with fast and robust development of the AD pathology hallmarks such as amyloid plaques and dystrophic neurites. We showed that even the short treatment with FO can affect the microglia clustering around amyloid plaques and increase the microglial plaque envelopment. Consequently, the Al2 accumulation was reduced and the appearance of DNs substantially suppressed. Our findings suggest that increased DHA consumption may play and important role in modulating microglial response and ameliorating AD pathology at least in the early phase of the disease.
PB  - Alzheimer's Association
C3  - AAIC Satellite Symposium: Aging and Alzheimer’s Disease: Opportunities for Therapeutic Interventions; 2017 Oct 19; Varna, Bulgaria
T1  - Short-term fish oil treatment suppresses the development of dystrophic neurites in the parietal cortex of 5XFAD AD mouse model during the early phase of the disease
SP  - 4B
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5715
ER  - 

@conference{
author = "Lončarević-Vasiljković, Nataša and Jović, Milena and Ivković, Sanja and Milanović, Desanka and Brkić, Marjana and Avramović, Vladimir and Kanazir, Selma",
year = "2017",
abstract = "Dystrophic neurites (DNs) are one of the neuropathological characteristics of Alzheimer's disease (AD) and represent the initial phase of neurodegeneration. Microtubule disruption in presynaptic dystrophic neurites that surround plaques impairs axonal transport and leads to the exacerbation of amyloid pathology in AD. Microglia plays a pivotal role in AD pathology as it is able to constitute a physical barrier around amyoid plaques and limit the accumulation of protofibrilar amylod beta around the fibrillar plaque core. In such a way microglia can mechanically shield the surrounding neurites from the neurotoxic protofibrillar Ai' aggregates. The use of supplements with omega-3 ( I%03) fatty acids (FAs), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), such as fish oil, is widespread due to proposed beneficial effects on the nervous system, High DHA consumption has been also associated with reduced risk and lessened AD pathology, yet the mechanisms and therapeutic potential of these supplements remain elusive. We analyzed the effects of the short-term fish oil (FO) supplementation on 4 months old 5xFAD mice, a mouse model with fast and robust development of the AD pathology hallmarks such as amyloid plaques and dystrophic neurites. We showed that even the short treatment with FO can affect the microglia clustering around amyloid plaques and increase the microglial plaque envelopment. Consequently, the Al2 accumulation was reduced and the appearance of DNs substantially suppressed. Our findings suggest that increased DHA consumption may play and important role in modulating microglial response and ameliorating AD pathology at least in the early phase of the disease.",
publisher = "Alzheimer's Association",
journal = "AAIC Satellite Symposium: Aging and Alzheimer’s Disease: Opportunities for Therapeutic Interventions; 2017 Oct 19; Varna, Bulgaria",
title = "Short-term fish oil treatment suppresses the development of dystrophic neurites in the parietal cortex of 5XFAD AD mouse model during the early phase of the disease",
pages = "4B",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5715"
}

Lončarević-Vasiljković, N., Jović, M., Ivković, S., Milanović, D., Brkić, M., Avramović, V.,& Kanazir, S.. (2017). Short-term fish oil treatment suppresses the development of dystrophic neurites in the parietal cortex of 5XFAD AD mouse model during the early phase of the disease. in AAIC Satellite Symposium: Aging and Alzheimer’s Disease: Opportunities for Therapeutic Interventions; 2017 Oct 19; Varna, Bulgaria
Alzheimer's Association., 4B.
https://hdl.handle.net/21.15107/rcub_ibiss_5715

Lončarević-Vasiljković N, Jović M, Ivković S, Milanović D, Brkić M, Avramović V, Kanazir S. Short-term fish oil treatment suppresses the development of dystrophic neurites in the parietal cortex of 5XFAD AD mouse model during the early phase of the disease. in AAIC Satellite Symposium: Aging and Alzheimer’s Disease: Opportunities for Therapeutic Interventions; 2017 Oct 19; Varna, Bulgaria. 2017;:4B.
https://hdl.handle.net/21.15107/rcub_ibiss_5715 .

Lončarević-Vasiljković, Nataša, Jović, Milena, Ivković, Sanja, Milanović, Desanka, Brkić, Marjana, Avramović, Vladimir, Kanazir, Selma, "Short-term fish oil treatment suppresses the development of dystrophic neurites in the parietal cortex of 5XFAD AD mouse model during the early phase of the disease" in AAIC Satellite Symposium: Aging and Alzheimer’s Disease: Opportunities for Therapeutic Interventions; 2017 Oct 19; Varna, Bulgaria (2017):4B,
https://hdl.handle.net/21.15107/rcub_ibiss_5715 .

TY  - CONF
AU  - Avramović, Vladimir
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Babić, Nikolina
AU  - Jović, Milena
AU  - Perović, Milka
AU  - Tešić, Vesna
AU  - Ivković, Sanja
AU  - Kanazir, Selma
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5829
AB  - Clinico-pathological features of Alzheimer's disease (AD) include memory loss, cognitive impairment, depression and social isolation. Epidemiological studies show a positive role of omega-3 poly-unsaturated fatty acids (n-3 PU FA) on prevention and mitigation of mild AD pathology. The 5xFAD mouse model used in this study bears five mutations linked to familiar forms of AD and recapitulates in a few months the main features of AD. This study was aimed to evaluate the effect of pre- and peri-natal omega-3 fatty acids supplementation on cognitive and non-cognitive behavior of 5xFAD mice . The fish oil, an abundant source of n-3 PUFA, was supplemented via oral gavages five days per week to dames throughout whole pregnancy and lactation. Six-month old offsprings were subjected to a battery of behavioral tests in order to assess typical rodent behavior, general locomotor activity, memory, depressive-like and social behavior. We have observed that the fish oil-supplemented 5xFAD offspring showed no changes in tests assessing typical rodent behavior, such as marble and nesting test, in comparison with 5XFAD control mice. When assessing depression and anxiety like behavior in a light-dark box, forced swim and open field behavioral tests significant changes were observed only between non-transgenic and transgenic mice. However, in three chambers test, used for assessing social behavior, fish oil- treated 5xFAD mice showed a significant increase in sociability, evaluated by increased time spent in compartment with, a mouse vs. empty compartment. Moreover, when the social novelty was tested through the introduction of a new mouse, the same trend was observed. This study shows that the pre- and peri-natal fish oil supplementation in 5xFAD mice can exert long-lasting effects inducing the significant improvement in some of the behavioral aspects of AD pathology in the adult offspring.
PB  - Belgrade: Serbian Nutrition Society
C3  - Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia
T1  - Fish oil supplementation during pre- and peri-natal period: Behavioral phenotyping of transgenic mouse model of Alzheimer’s disease
SP  - 257
EP  - 258
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5829
ER  - 

@conference{
author = "Avramović, Vladimir and Milanović, Desanka and Brkić, Marjana and Babić, Nikolina and Jović, Milena and Perović, Milka and Tešić, Vesna and Ivković, Sanja and Kanazir, Selma",
year = "2016",
abstract = "Clinico-pathological features of Alzheimer's disease (AD) include memory loss, cognitive impairment, depression and social isolation. Epidemiological studies show a positive role of omega-3 poly-unsaturated fatty acids (n-3 PU FA) on prevention and mitigation of mild AD pathology. The 5xFAD mouse model used in this study bears five mutations linked to familiar forms of AD and recapitulates in a few months the main features of AD. This study was aimed to evaluate the effect of pre- and peri-natal omega-3 fatty acids supplementation on cognitive and non-cognitive behavior of 5xFAD mice . The fish oil, an abundant source of n-3 PUFA, was supplemented via oral gavages five days per week to dames throughout whole pregnancy and lactation. Six-month old offsprings were subjected to a battery of behavioral tests in order to assess typical rodent behavior, general locomotor activity, memory, depressive-like and social behavior. We have observed that the fish oil-supplemented 5xFAD offspring showed no changes in tests assessing typical rodent behavior, such as marble and nesting test, in comparison with 5XFAD control mice. When assessing depression and anxiety like behavior in a light-dark box, forced swim and open field behavioral tests significant changes were observed only between non-transgenic and transgenic mice. However, in three chambers test, used for assessing social behavior, fish oil- treated 5xFAD mice showed a significant increase in sociability, evaluated by increased time spent in compartment with, a mouse vs. empty compartment. Moreover, when the social novelty was tested through the introduction of a new mouse, the same trend was observed. This study shows that the pre- and peri-natal fish oil supplementation in 5xFAD mice can exert long-lasting effects inducing the significant improvement in some of the behavioral aspects of AD pathology in the adult offspring.",
publisher = "Belgrade: Serbian Nutrition Society",
journal = "Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia",
title = "Fish oil supplementation during pre- and peri-natal period: Behavioral phenotyping of transgenic mouse model of Alzheimer’s disease",
pages = "257-258",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5829"
}

Avramović, V., Milanović, D., Brkić, M., Babić, N., Jović, M., Perović, M., Tešić, V., Ivković, S.,& Kanazir, S.. (2016). Fish oil supplementation during pre- and peri-natal period: Behavioral phenotyping of transgenic mouse model of Alzheimer’s disease. in Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia
Belgrade: Serbian Nutrition Society., 257-258.
https://hdl.handle.net/21.15107/rcub_ibiss_5829

Avramović V, Milanović D, Brkić M, Babić N, Jović M, Perović M, Tešić V, Ivković S, Kanazir S. Fish oil supplementation during pre- and peri-natal period: Behavioral phenotyping of transgenic mouse model of Alzheimer’s disease. in Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia. 2016;:257-258.
https://hdl.handle.net/21.15107/rcub_ibiss_5829 .

Avramović, Vladimir, Milanović, Desanka, Brkić, Marjana, Babić, Nikolina, Jović, Milena, Perović, Milka, Tešić, Vesna, Ivković, Sanja, Kanazir, Selma, "Fish oil supplementation during pre- and peri-natal period: Behavioral phenotyping of transgenic mouse model of Alzheimer’s disease" in Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia (2016):257-258,
https://hdl.handle.net/21.15107/rcub_ibiss_5829 .