TY  - CHAP
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Petri, Edward
AU  - Popović, Željko D.
AU  - Grubor-Lajšić, Gordana
AU  - Blagojević, Duško
AU  - Raman, Chandrasekar
AU  - Goldsmith, Marian R.
AU  - Agunbiade, Tolulope A.
PY  - 2016
UR  - http://link.springer.com/10.1007/978-3-319-24244-6_4
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2610
AB  - In this chapter, we review recent genomic, proteomic, and metabolomic studies that link several gene and protein products involved in cold adaptation in insects and other arthropods to energy metabolism and cellular protection mechanisms. Organisms have evolved various mechanisms for survival at subfreezing temperatures. In general, cold hardy invertebrates utilize four main strategies to survive cold temperatures: (1) freeze tolerance, (2) freeze avoidance, (3) cryoprotective dehydration, and (4) vitrification. In addition, many insects in temperate regions overwinter in an arrested developmental state known as diapause, during which they are cold hardy. Major alterations occur during winter diapause, with respect to both total metabolic flux and the relative activities of different metabolic pathways. In these organisms, one such metabolic adaptation to unfavorably cold environmental conditions is the synthesis of cryoprotectants/anhydroprotectants. The metabolic changes and metabolic paths involved in cold adaptation suggest involvement of specific enzymes and key regulatory proteins. These mechanisms of cold adaptation require precise scheduling of the expression of specific genes. Thus, we discuss here the evidence researchers have recently begun to gather supporting a relationship between the genes and proteins of the cold adaptation response and mechanisms of cellular protection and energy metabolism using an “omics” approach.
PB  - Springer International Publishing
T2  - Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2
T1  - Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach
DO  - 10.1007/978-3-319-24244-6_4
SP  - 89
EP  - 112
ER  - 

@inbook{
author = "Purać, Jelena and Kojić, Danijela and Petri, Edward and Popović, Željko D. and Grubor-Lajšić, Gordana and Blagojević, Duško and Raman, Chandrasekar and Goldsmith, Marian R. and Agunbiade, Tolulope A.",
year = "2016",
abstract = "In this chapter, we review recent genomic, proteomic, and metabolomic studies that link several gene and protein products involved in cold adaptation in insects and other arthropods to energy metabolism and cellular protection mechanisms. Organisms have evolved various mechanisms for survival at subfreezing temperatures. In general, cold hardy invertebrates utilize four main strategies to survive cold temperatures: (1) freeze tolerance, (2) freeze avoidance, (3) cryoprotective dehydration, and (4) vitrification. In addition, many insects in temperate regions overwinter in an arrested developmental state known as diapause, during which they are cold hardy. Major alterations occur during winter diapause, with respect to both total metabolic flux and the relative activities of different metabolic pathways. In these organisms, one such metabolic adaptation to unfavorably cold environmental conditions is the synthesis of cryoprotectants/anhydroprotectants. The metabolic changes and metabolic paths involved in cold adaptation suggest involvement of specific enzymes and key regulatory proteins. These mechanisms of cold adaptation require precise scheduling of the expression of specific genes. Thus, we discuss here the evidence researchers have recently begun to gather supporting a relationship between the genes and proteins of the cold adaptation response and mechanisms of cellular protection and energy metabolism using an “omics” approach.",
publisher = "Springer International Publishing",
journal = "Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2",
booktitle = "Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach",
doi = "10.1007/978-3-319-24244-6_4",
pages = "89-112"
}

Purać, J., Kojić, D., Petri, E., Popović, Ž. D., Grubor-Lajšić, G., Blagojević, D., Raman, C., Goldsmith, M. R.,& Agunbiade, T. A.. (2016). Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach. in Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2
Springer International Publishing., 89-112.
https://doi.org/10.1007/978-3-319-24244-6_4

Purać J, Kojić D, Petri E, Popović ŽD, Grubor-Lajšić G, Blagojević D, Raman C, Goldsmith MR, Agunbiade TA. Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach. in Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2. 2016;:89-112.
doi:10.1007/978-3-319-24244-6_4 .

Purać, Jelena, Kojić, Danijela, Petri, Edward, Popović, Željko D., Grubor-Lajšić, Gordana, Blagojević, Duško, Raman, Chandrasekar, Goldsmith, Marian R., Agunbiade, Tolulope A., "Cold Adaptation Responses in Insects and Other Arthropods: An “Omics” Approach" in Short Views on Insect Genomics and Proteomics: Insect Proteomics. Vol. 2 (2016):89-112,
https://doi.org/10.1007/978-3-319-24244-6_4 . .

TY  - JOUR
AU  - Grubor-Lajšić, Gordana
AU  - Petri, Edward
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Popović, Željko
AU  - Worland, Michael Roger
AU  - Clark, Melody S.
AU  - Mojović, Miloš
AU  - Blagojević, Duško
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6338
AB  - The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H2O2), or dehydration, is well documented. In this respect, we measured the amount of H2O2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H2O2 concentrations, in Arctic springtails incubated at 5 ◦C (control) versus −2 ◦C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in
Drosophila, we measured the levels of the active physiological form of ecdysone—20-hydroxyecdysone (20-HE). Significantly elevated H2O2 and 20-HE levels were observed in M. arctica incubated at −2 ◦C, supporting a link between ecdysone, H2O2, and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 ◦C versus 5 ◦C, suggesting reduced H2O2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 ◦C (controls) versus −2 ◦C by Electron Paramagnetic Resonance spectroscopy revealed melanin-derived free radicals at −2 ◦C, perhaps an additional source of H2O2. Our results suggest that H2O2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis.
PB  - Hoboken: John Wiley and Sons
T2  - Archives of Insect Biochemistry and Physiology
T1  - Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)
IS  - 2
VL  - 82
DO  - 10.1002/arch.21073
SP  - 59
EP  - 70
ER  - 

@article{
author = "Grubor-Lajšić, Gordana and Petri, Edward and Kojić, Danijela and Purać, Jelena and Popović, Željko and Worland, Michael Roger and Clark, Melody S. and Mojović, Miloš and Blagojević, Duško",
year = "2013",
abstract = "The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H2O2), or dehydration, is well documented. In this respect, we measured the amount of H2O2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H2O2 concentrations, in Arctic springtails incubated at 5 ◦C (control) versus −2 ◦C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in
Drosophila, we measured the levels of the active physiological form of ecdysone—20-hydroxyecdysone (20-HE). Significantly elevated H2O2 and 20-HE levels were observed in M. arctica incubated at −2 ◦C, supporting a link between ecdysone, H2O2, and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 ◦C versus 5 ◦C, suggesting reduced H2O2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 ◦C (controls) versus −2 ◦C by Electron Paramagnetic Resonance spectroscopy revealed melanin-derived free radicals at −2 ◦C, perhaps an additional source of H2O2. Our results suggest that H2O2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Archives of Insect Biochemistry and Physiology",
title = "Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)",
number = "2",
volume = "82",
doi = "10.1002/arch.21073",
pages = "59-70"
}

Grubor-Lajšić, G., Petri, E., Kojić, D., Purać, J., Popović, Ž., Worland, M. R., Clark, M. S., Mojović, M.,& Blagojević, D.. (2013). Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola). in Archives of Insect Biochemistry and Physiology
Hoboken: John Wiley and Sons., 82(2), 59-70.
https://doi.org/10.1002/arch.21073

Grubor-Lajšić G, Petri E, Kojić D, Purać J, Popović Ž, Worland MR, Clark MS, Mojović M, Blagojević D. Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola). in Archives of Insect Biochemistry and Physiology. 2013;82(2):59-70.
doi:10.1002/arch.21073 .

Grubor-Lajšić, Gordana, Petri, Edward, Kojić, Danijela, Purać, Jelena, Popović, Željko, Worland, Michael Roger, Clark, Melody S., Mojović, Miloš, Blagojević, Duško, "Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)" in Archives of Insect Biochemistry and Physiology, 82, no. 2 (2013):59-70,
https://doi.org/10.1002/arch.21073 . .