TY  - JOUR
AU  - Ćalić, Dušica
AU  - Zdravković-Korać, Snežana
AU  - Bohanec, Borut
AU  - Radojević, Ljiljana
AU  - Vinterhalter, Branka
AU  - Stevović, Svetlana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
PY  - 2010
UR  - https://www.ajol.info/index.php/ajb/article/view/82495
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6169
AB  - The influence of activated charcoal (AC), abscisic acid (ABA) and polyethylene glycol (PEG) on the maturation and conversion of horse chestnut (Aesculus hippocastanum L.) androgenic embryos were tested. Androgenic embryos originating from microspores and anther culture were maturated over 90 days. Androgenic embryos on media containing PEG (50 g/l), in combination with AC (1 g/l) showed a rapid development of embryos in the cotyledonary stage and lowered percentage of abnormal structures. The best results of androgenic microspore embryo germination were observed on media supplemented with AC alone (99%) and in combination with PEG (100%). Also, the greatest number of androgenic microspore plants (18%) and androgenic anther plants (12%) were formed on media enriched with 1 % AC. Lowest germination percentages of 37 and 39% in microspore culture and 33 and 38% in anther culture were obtained on maturation media with ABA 20 mg/l alone and in combination with AC 1g/l. Flow cytometric analysis showed that most of the androgenic embryos were haploid, corresponding to their microspore origin, while half of these became diploid after maturation for 90 days. All regenerants originating from microspore culture were haploid immediately after germination, but only 10% embryos retained haploidity after 3 years subculturing, while 10.5% were diploid, 73.5% tetraploid and 6% octaploid on hormone-free medium. Unlike those from anther culture, after 3 years of subculturing on hormone-free medium, there were no haploid regenerant from anther culture, while 8.5% were diploid, 81% tetraploid and 10.5% octaploid.
PB  - Nairobi: Academic Journals
T2  - African Journal of Biotechnology
T1  - Effect of activated charcoal, abscisic acid and polyethylene glycol on maturation, germination and conversion of Aesculus hippocastanum androgenic embryos
IS  - 25
VL  - 9
SP  - 3786
EP  - 3793
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6169
ER  - 

@article{
author = "Ćalić, Dušica and Zdravković-Korać, Snežana and Bohanec, Borut and Radojević, Ljiljana and Vinterhalter, Branka and Stevović, Svetlana and Cingel, Aleksandar and Savić, Jelena",
year = "2010",
abstract = "The influence of activated charcoal (AC), abscisic acid (ABA) and polyethylene glycol (PEG) on the maturation and conversion of horse chestnut (Aesculus hippocastanum L.) androgenic embryos were tested. Androgenic embryos originating from microspores and anther culture were maturated over 90 days. Androgenic embryos on media containing PEG (50 g/l), in combination with AC (1 g/l) showed a rapid development of embryos in the cotyledonary stage and lowered percentage of abnormal structures. The best results of androgenic microspore embryo germination were observed on media supplemented with AC alone (99%) and in combination with PEG (100%). Also, the greatest number of androgenic microspore plants (18%) and androgenic anther plants (12%) were formed on media enriched with 1 % AC. Lowest germination percentages of 37 and 39% in microspore culture and 33 and 38% in anther culture were obtained on maturation media with ABA 20 mg/l alone and in combination with AC 1g/l. Flow cytometric analysis showed that most of the androgenic embryos were haploid, corresponding to their microspore origin, while half of these became diploid after maturation for 90 days. All regenerants originating from microspore culture were haploid immediately after germination, but only 10% embryos retained haploidity after 3 years subculturing, while 10.5% were diploid, 73.5% tetraploid and 6% octaploid on hormone-free medium. Unlike those from anther culture, after 3 years of subculturing on hormone-free medium, there were no haploid regenerant from anther culture, while 8.5% were diploid, 81% tetraploid and 10.5% octaploid.",
publisher = "Nairobi: Academic Journals",
journal = "African Journal of Biotechnology",
title = "Effect of activated charcoal, abscisic acid and polyethylene glycol on maturation, germination and conversion of Aesculus hippocastanum androgenic embryos",
number = "25",
volume = "9",
pages = "3786-3793",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6169"
}

Ćalić, D., Zdravković-Korać, S., Bohanec, B., Radojević, L., Vinterhalter, B., Stevović, S., Cingel, A.,& Savić, J.. (2010). Effect of activated charcoal, abscisic acid and polyethylene glycol on maturation, germination and conversion of Aesculus hippocastanum androgenic embryos. in African Journal of Biotechnology
Nairobi: Academic Journals., 9(25), 3786-3793.
https://hdl.handle.net/21.15107/rcub_ibiss_6169

Ćalić D, Zdravković-Korać S, Bohanec B, Radojević L, Vinterhalter B, Stevović S, Cingel A, Savić J. Effect of activated charcoal, abscisic acid and polyethylene glycol on maturation, germination and conversion of Aesculus hippocastanum androgenic embryos. in African Journal of Biotechnology. 2010;9(25):3786-3793.
https://hdl.handle.net/21.15107/rcub_ibiss_6169 .

Ćalić, Dušica, Zdravković-Korać, Snežana, Bohanec, Borut, Radojević, Ljiljana, Vinterhalter, Branka, Stevović, Svetlana, Cingel, Aleksandar, Savić, Jelena, "Effect of activated charcoal, abscisic acid and polyethylene glycol on maturation, germination and conversion of Aesculus hippocastanum androgenic embryos" in African Journal of Biotechnology, 9, no. 25 (2010):3786-3793,
https://hdl.handle.net/21.15107/rcub_ibiss_6169 .

TY  - CONF
AU  - Ćalić, Dušica
AU  - Zdravković-Korać, Snežana
AU  - Devrnja, Nina
AU  - Radojević, Ljiljana
AU  - Vinterhalter, Branka
PY  - 2010
UR  - http://www.forest.org.rs/pdf/konferencije/PROCEEDINGS-Vol2-FOREST-ECOSYSTEMSAND-CLIMATE-CHANGES.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6153
AB  - Nevertheless, cultivated A. flava tree, about 15 years old, growing in the Botanical Garden "Jevremovac" of the Belgrade University. Closed flower buds (4-5 mm long) with premature anthers were harvested from A. flava tree. Androgenesis was induced in anther culture on solid MS (Murashige and Skoog, 1962) medium with 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (1.0 mg/l of each). Globular androgenic embryos were appeared after two months. However, after globular embryos, heart, torpedo-like and cotyledonal embryos were appeared. Feature development and multiplication of androgenic embryos proceed on a solid medium with reduced concentration of 2,4-D (0.01 mg/l) and same concentration of kinetin. After medium for multiplication, embryos were cultured on media for embryo maturation supplemented with various concentrations (0.1, 0.5, 1, 2 and 3 %) of activated charcoal. The effect of activated charcoal has been attributed to the absorption of inhibitory substances, such as phenolic compounds which are produced by embryos, from the medium. The best results of germination and maturation of A. flava androgenic embryos were obtained on medium supplemented with 1 % activated charcoal. Also, the greatest number of secondary somatic embryos and the lowest number of albino embryos were noticed on the same medium.
This is the first report about induction and maturation androgenic embryos originating from A. flava anther culture.
PB  - Belgrade: Institute of Forestry
C3  - Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia
T1  - Maturation of Aesculus flava Marshall. androgenic embryos
SP  - 55
EP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6153
ER  - 

@conference{
author = "Ćalić, Dušica and Zdravković-Korać, Snežana and Devrnja, Nina and Radojević, Ljiljana and Vinterhalter, Branka",
year = "2010",
abstract = "Nevertheless, cultivated A. flava tree, about 15 years old, growing in the Botanical Garden "Jevremovac" of the Belgrade University. Closed flower buds (4-5 mm long) with premature anthers were harvested from A. flava tree. Androgenesis was induced in anther culture on solid MS (Murashige and Skoog, 1962) medium with 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (1.0 mg/l of each). Globular androgenic embryos were appeared after two months. However, after globular embryos, heart, torpedo-like and cotyledonal embryos were appeared. Feature development and multiplication of androgenic embryos proceed on a solid medium with reduced concentration of 2,4-D (0.01 mg/l) and same concentration of kinetin. After medium for multiplication, embryos were cultured on media for embryo maturation supplemented with various concentrations (0.1, 0.5, 1, 2 and 3 %) of activated charcoal. The effect of activated charcoal has been attributed to the absorption of inhibitory substances, such as phenolic compounds which are produced by embryos, from the medium. The best results of germination and maturation of A. flava androgenic embryos were obtained on medium supplemented with 1 % activated charcoal. Also, the greatest number of secondary somatic embryos and the lowest number of albino embryos were noticed on the same medium.
This is the first report about induction and maturation androgenic embryos originating from A. flava anther culture.",
publisher = "Belgrade: Institute of Forestry",
journal = "Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia",
title = "Maturation of Aesculus flava Marshall. androgenic embryos",
pages = "55-59",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6153"
}

Ćalić, D., Zdravković-Korać, S., Devrnja, N., Radojević, L.,& Vinterhalter, B.. (2010). Maturation of Aesculus flava Marshall. androgenic embryos. in Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia
Belgrade: Institute of Forestry., 55-59.
https://hdl.handle.net/21.15107/rcub_ibiss_6153

Ćalić D, Zdravković-Korać S, Devrnja N, Radojević L, Vinterhalter B. Maturation of Aesculus flava Marshall. androgenic embryos. in Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia. 2010;:55-59.
https://hdl.handle.net/21.15107/rcub_ibiss_6153 .

Ćalić, Dušica, Zdravković-Korać, Snežana, Devrnja, Nina, Radojević, Ljiljana, Vinterhalter, Branka, "Maturation of Aesculus flava Marshall. androgenic embryos" in Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia (2010):55-59,
https://hdl.handle.net/21.15107/rcub_ibiss_6153 .

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana
AU  - Vinterhalter, Branka
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6152
AB  - Anthers of A. flava were isolated from disinfected flower buds and cultivated on solid callus induction medium (CIM), containing Murashige and Skoog's (MS) mineral solution with 1 mg/l 2,4 dichlorophenoxyacetic acid (2,4 D) and 1 mg/l 6 furfurylaminopurine (Kin). A half of anthers was kept on solid CIM medium and the other half was macerated 3-5 days later, to create microspore suspension cultures. The microspores released from anthers were suspended in liquid CIM medium, sieved through a 50 μm mesh and shaken on a platform shaker. Eight weeks later, anthers were transferred to regeneration induction medium (RIM) containing MS salts, 0.01 mg/l 2,4 D and 1 mg/l Kin. Microspore suspensions were mixed with equal volumes of cooled RIM and the mixture was dispensed in Petri dishes. Embryo emergence from both types of cultures had been monitored over a 2-month period. The difference in embryogenic potential of anthers positioned along the inflorescence's axis was observed and it declined acropetally. Anthers isolated from basal third of inflorescence (A-anthers) exhibited the highest frequency of embryo formation (36.6%), comparing to anthers from the middle third of inflorescence (B-anthers) and particularly the top third anthers (C-anthers). The embryo average number per anther did not differ significantly between A- and B-anthers, whereas it was significantly lower in C-anthers. The same tendency was observed in microspore suspension cultures. Microspore suspension culture was more efficient method for embryo induction, as it yielded twice more embryos then anther culture. Androgenic embryos obtained by both techniques were maintained and multiplied by repetitive somatic embryogenesis on solid RIM medium.
PB  - Belgrade: Institute of Forestry
C3  - Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia
T1  - A comparison between anther culture and microspore suspension culture of Aesculus flava
SP  - 49
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6152
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Ćalić, Dušica and Milojević, Jelena and Tubić, Ljiljana and Vinterhalter, Branka",
year = "2010",
abstract = "Anthers of A. flava were isolated from disinfected flower buds and cultivated on solid callus induction medium (CIM), containing Murashige and Skoog's (MS) mineral solution with 1 mg/l 2,4 dichlorophenoxyacetic acid (2,4 D) and 1 mg/l 6 furfurylaminopurine (Kin). A half of anthers was kept on solid CIM medium and the other half was macerated 3-5 days later, to create microspore suspension cultures. The microspores released from anthers were suspended in liquid CIM medium, sieved through a 50 μm mesh and shaken on a platform shaker. Eight weeks later, anthers were transferred to regeneration induction medium (RIM) containing MS salts, 0.01 mg/l 2,4 D and 1 mg/l Kin. Microspore suspensions were mixed with equal volumes of cooled RIM and the mixture was dispensed in Petri dishes. Embryo emergence from both types of cultures had been monitored over a 2-month period. The difference in embryogenic potential of anthers positioned along the inflorescence's axis was observed and it declined acropetally. Anthers isolated from basal third of inflorescence (A-anthers) exhibited the highest frequency of embryo formation (36.6%), comparing to anthers from the middle third of inflorescence (B-anthers) and particularly the top third anthers (C-anthers). The embryo average number per anther did not differ significantly between A- and B-anthers, whereas it was significantly lower in C-anthers. The same tendency was observed in microspore suspension cultures. Microspore suspension culture was more efficient method for embryo induction, as it yielded twice more embryos then anther culture. Androgenic embryos obtained by both techniques were maintained and multiplied by repetitive somatic embryogenesis on solid RIM medium.",
publisher = "Belgrade: Institute of Forestry",
journal = "Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia",
title = "A comparison between anther culture and microspore suspension culture of Aesculus flava",
pages = "49-54",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6152"
}

Zdravković-Korać, S., Ćalić, D., Milojević, J., Tubić, L.,& Vinterhalter, B.. (2010). A comparison between anther culture and microspore suspension culture of Aesculus flava. in Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia
Belgrade: Institute of Forestry., 49-54.
https://hdl.handle.net/21.15107/rcub_ibiss_6152

Zdravković-Korać S, Ćalić D, Milojević J, Tubić L, Vinterhalter B. A comparison between anther culture and microspore suspension culture of Aesculus flava. in Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia. 2010;:49-54.
https://hdl.handle.net/21.15107/rcub_ibiss_6152 .

Zdravković-Korać, Snežana, Ćalić, Dušica, Milojević, Jelena, Tubić, Ljiljana, Vinterhalter, Branka, "A comparison between anther culture and microspore suspension culture of Aesculus flava" in Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia (2010):49-54,
https://hdl.handle.net/21.15107/rcub_ibiss_6152 .

TY  - CONF
AU  - Ćalić, Dušica
AU  - Zdravković-Korać, Snežana
AU  - Radojević, Ljiljana
PY  - 2010
UR  - http://www.forest.org.rs/pdf/konferencije/PROCEEDINGS-Vol2-FOREST-ECOSYSTEMSAND-CLIMATE-CHANGES.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6154
AB  - Influence of different genotype, age of trees and environmental temperature on induction of androgenesis and apperance of albino horse chestnut embryos were studied. Efficiency of in vitro androgenesis via anther and microspore culture were investigated using the same closed flower bud material. Androgenic response of different genotypes was measured and compared. Anther induction rates were from 5 % to 37.6 % depending of genotype. Under optimal conditions, the number of embryos per isolated anther varied between 0.5 to 5.0 embryos in anther culture, while in microspore culture varied between 3.0 to 27 embryos, depending of genotype. A microspore culture was 5-6 times more efficient than anther culture for the same genotype. Age of the trees had no influence on androgenesis induction. Temperature of about 4-5°C was optimal for androgenic embryo induction. Flow cytometric analysis of embryos and regenerated plants showed that the most of the androgenic embryos were haploid, corresponding to their microspore origin, while a half of these were diploid, after 6 months in culture. However, diploid, tetraploid and octaploid embryos were observed after 3 years in subculturing.
PB  - Belgrade: Institute of Forestry
C3  - Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia
T1  - The effect of genotype on androgenesis induction of Aesculus hippocastanum L.
SP  - 71
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6154
ER  - 

@conference{
author = "Ćalić, Dušica and Zdravković-Korać, Snežana and Radojević, Ljiljana",
year = "2010",
abstract = "Influence of different genotype, age of trees and environmental temperature on induction of androgenesis and apperance of albino horse chestnut embryos were studied. Efficiency of in vitro androgenesis via anther and microspore culture were investigated using the same closed flower bud material. Androgenic response of different genotypes was measured and compared. Anther induction rates were from 5 % to 37.6 % depending of genotype. Under optimal conditions, the number of embryos per isolated anther varied between 0.5 to 5.0 embryos in anther culture, while in microspore culture varied between 3.0 to 27 embryos, depending of genotype. A microspore culture was 5-6 times more efficient than anther culture for the same genotype. Age of the trees had no influence on androgenesis induction. Temperature of about 4-5°C was optimal for androgenic embryo induction. Flow cytometric analysis of embryos and regenerated plants showed that the most of the androgenic embryos were haploid, corresponding to their microspore origin, while a half of these were diploid, after 6 months in culture. However, diploid, tetraploid and octaploid embryos were observed after 3 years in subculturing.",
publisher = "Belgrade: Institute of Forestry",
journal = "Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia",
title = "The effect of genotype on androgenesis induction of Aesculus hippocastanum L.",
pages = "71-76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6154"
}

Ćalić, D., Zdravković-Korać, S.,& Radojević, L.. (2010). The effect of genotype on androgenesis induction of Aesculus hippocastanum L.. in Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia
Belgrade: Institute of Forestry., 71-76.
https://hdl.handle.net/21.15107/rcub_ibiss_6154

Ćalić D, Zdravković-Korać S, Radojević L. The effect of genotype on androgenesis induction of Aesculus hippocastanum L.. in Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia. 2010;:71-76.
https://hdl.handle.net/21.15107/rcub_ibiss_6154 .

Ćalić, Dušica, Zdravković-Korać, Snežana, Radojević, Ljiljana, "The effect of genotype on androgenesis induction of Aesculus hippocastanum L." in Proceedings: International Scientific Conference: Forest Ecosystems and Climate Changes: Volume 2; 2010 Mar 9-10; Belgrade, Serbia (2010):71-76,
https://hdl.handle.net/21.15107/rcub_ibiss_6154 .

TY  - JOUR
AU  - Zdravković-Korać, Snežana
AU  - Ćalić-Dragosavac, Dušica D.
AU  - Uzelac, Branka
AU  - Janošević, Dušica A.
AU  - Budimir, Snežana
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1523
AB  - Somatic embryos of red horse chestnut, derived from cultures maintained through repetitive somatic embryogenesis for a few years, were subjected to induction of secondary regeneration. The embryos were divided in four classes on the basis of their size (I-1, II-5, III-10 and IV-30 mm), and sub-cultured on MS media containing 0, 1, 5 or 10 mu M kinetin (Kin) or benzyladenine (BA). The pathway of secondary regeneration, somatic embryogenesis or caulogenesis, depended on the primary somatic embryo (PSE) stage of development. The embryogenic capacity declined and bud-forming capacity increased with the degree of PSE maturity. The PSE of the Classes I and II produced only secondary somatic embryos (SSE), the Class III PSE formed both SSE and adventitious buds, whereas the Class IV PSE developed almost solely adventitious buds. The process of secondary somatic embryogenesis was most effective in the Class II PSE at 5 mu M BA, and the process of adventive organogenesis was most effective in the Class IV PSE at 10 mu M BA. On plant growth regulator (PGR)-free medium, PSE of A. carnea followed the same pattern of adventive regeneration, as those cultured on cytokinin containing media. The cytokinins only amplified the response, in a certain range of concentrations. BA promoted bud induction at a much higher rate than Kin, while their embryogenic effect was similar.
T2  - Plant Cell Tissue and Organ Culture
T1  - Secondary somatic embryogenesis versus caulogenesis from somatic embryos of Aesculus carnea Hayne.: developmental stage impact
IS  - 2
VL  - 94
DO  - 10.1007/s11240-008-9399-4
EP  - 231
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1523
ER  - 

@article{
author = "Zdravković-Korać, Snežana and Ćalić-Dragosavac, Dušica D. and Uzelac, Branka and Janošević, Dušica A. and Budimir, Snežana and Vinterhalter, Branka and Vinterhalter, Dragan",
year = "2008",
abstract = "Somatic embryos of red horse chestnut, derived from cultures maintained through repetitive somatic embryogenesis for a few years, were subjected to induction of secondary regeneration. The embryos were divided in four classes on the basis of their size (I-1, II-5, III-10 and IV-30 mm), and sub-cultured on MS media containing 0, 1, 5 or 10 mu M kinetin (Kin) or benzyladenine (BA). The pathway of secondary regeneration, somatic embryogenesis or caulogenesis, depended on the primary somatic embryo (PSE) stage of development. The embryogenic capacity declined and bud-forming capacity increased with the degree of PSE maturity. The PSE of the Classes I and II produced only secondary somatic embryos (SSE), the Class III PSE formed both SSE and adventitious buds, whereas the Class IV PSE developed almost solely adventitious buds. The process of secondary somatic embryogenesis was most effective in the Class II PSE at 5 mu M BA, and the process of adventive organogenesis was most effective in the Class IV PSE at 10 mu M BA. On plant growth regulator (PGR)-free medium, PSE of A. carnea followed the same pattern of adventive regeneration, as those cultured on cytokinin containing media. The cytokinins only amplified the response, in a certain range of concentrations. BA promoted bud induction at a much higher rate than Kin, while their embryogenic effect was similar.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Secondary somatic embryogenesis versus caulogenesis from somatic embryos of Aesculus carnea Hayne.: developmental stage impact",
number = "2",
volume = "94",
doi = "10.1007/s11240-008-9399-4",
pages = "231",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1523"
}

Zdravković-Korać, S., Ćalić-Dragosavac, D. D., Uzelac, B., Janošević, D. A., Budimir, S., Vinterhalter, B.,& Vinterhalter, D.. (2008). Secondary somatic embryogenesis versus caulogenesis from somatic embryos of Aesculus carnea Hayne.: developmental stage impact. in Plant Cell Tissue and Organ Culture, 94(2).
https://doi.org/10.1007/s11240-008-9399-4
https://hdl.handle.net/21.15107/rcub_ibiss_1523

Zdravković-Korać S, Ćalić-Dragosavac DD, Uzelac B, Janošević DA, Budimir S, Vinterhalter B, Vinterhalter D. Secondary somatic embryogenesis versus caulogenesis from somatic embryos of Aesculus carnea Hayne.: developmental stage impact. in Plant Cell Tissue and Organ Culture. 2008;94(2):null-231.
doi:10.1007/s11240-008-9399-4
https://hdl.handle.net/21.15107/rcub_ibiss_1523 .

Zdravković-Korać, Snežana, Ćalić-Dragosavac, Dušica D., Uzelac, Branka, Janošević, Dušica A., Budimir, Snežana, Vinterhalter, Branka, Vinterhalter, Dragan, "Secondary somatic embryogenesis versus caulogenesis from somatic embryos of Aesculus carnea Hayne.: developmental stage impact" in Plant Cell Tissue and Organ Culture, 94, no. 2 (2008),
https://doi.org/10.1007/s11240-008-9399-4 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1523 .

TY  - CONF
AU  - Ćalić, Dušica
AU  - Zdravković-Korać, Snežana
AU  - Radojević, Ljiljana
PY  - 2006
UR  - https://www.forest.org.rs/files/InternationalScientificConference2006-SUSTAINABLEUSEOFFORESTECOSYSTEMS.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6174
AB  - In the field of regeneration of plant in vitro cultures, haploid formation from pollen is the scientifically most advanced. In the present state of transition from basic research to commercial application, a sound scientific basis of pollen embryogenesis would make this transition much easier. New discoveries in recent years have made it possible to develop a new view of pollen embryogenesis. The new view includes recognition theoretical aspects, provides a model for a number of problems in plant development, and has consequences for strategies for haploid production. We developed an excellent model system for a mass production of haploid regenerants of Aesculus hippocastanum, Aesculus carnea, Aesculus flava, and Aesculus parviflora.
PB  - Belgrade: Institute of Forestry
C3  - Proceedings: International scientific conference sustainable use of forest ecosystems: the challenge of the 21st Century; 2006 Nov 8-10; Donji Milanovac, Serbia
T1  - Pollen embryogenesis of spp. Aesculus
SP  - 519
EP  - 523
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6174
ER  - 

@conference{
author = "Ćalić, Dušica and Zdravković-Korać, Snežana and Radojević, Ljiljana",
year = "2006",
abstract = "In the field of regeneration of plant in vitro cultures, haploid formation from pollen is the scientifically most advanced. In the present state of transition from basic research to commercial application, a sound scientific basis of pollen embryogenesis would make this transition much easier. New discoveries in recent years have made it possible to develop a new view of pollen embryogenesis. The new view includes recognition theoretical aspects, provides a model for a number of problems in plant development, and has consequences for strategies for haploid production. We developed an excellent model system for a mass production of haploid regenerants of Aesculus hippocastanum, Aesculus carnea, Aesculus flava, and Aesculus parviflora.",
publisher = "Belgrade: Institute of Forestry",
journal = "Proceedings: International scientific conference sustainable use of forest ecosystems: the challenge of the 21st Century; 2006 Nov 8-10; Donji Milanovac, Serbia",
title = "Pollen embryogenesis of spp. Aesculus",
pages = "519-523",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6174"
}

Ćalić, D., Zdravković-Korać, S.,& Radojević, L.. (2006). Pollen embryogenesis of spp. Aesculus. in Proceedings: International scientific conference sustainable use of forest ecosystems: the challenge of the 21st Century; 2006 Nov 8-10; Donji Milanovac, Serbia
Belgrade: Institute of Forestry., 519-523.
https://hdl.handle.net/21.15107/rcub_ibiss_6174

Ćalić D, Zdravković-Korać S, Radojević L. Pollen embryogenesis of spp. Aesculus. in Proceedings: International scientific conference sustainable use of forest ecosystems: the challenge of the 21st Century; 2006 Nov 8-10; Donji Milanovac, Serbia. 2006;:519-523.
https://hdl.handle.net/21.15107/rcub_ibiss_6174 .

Ćalić, Dušica, Zdravković-Korać, Snežana, Radojević, Ljiljana, "Pollen embryogenesis of spp. Aesculus" in Proceedings: International scientific conference sustainable use of forest ecosystems: the challenge of the 21st Century; 2006 Nov 8-10; Donji Milanovac, Serbia (2006):519-523,
https://hdl.handle.net/21.15107/rcub_ibiss_6174 .

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Ćalić, Dušica
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Vinterhalter, Branka
AU  - Radojević, Ljiljana
PY  - 2006
UR  - https://www.forest.org.rs/files/InternationalScientificConference2006-SUSTAINABLEUSEOFFORESTECOSYSTEMS.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6171
UR  - https://plus.cobiss.net/cobiss/sr/sr/bib/32558095
AB  - Somatic embryos of red horse chestnut were subjected to an induction of secondary regeneration. The embryos were divided in 4 classes on the basis of their size (I 1, II 5, III 10 and IV 30 mm), and sub-cultivated on MS media containing 0, 1, 5 or 10 μM kinetin (Kin) or benzyladenine (BA). The pathway of secondary regeneration (somatic embryogenesis or caulogenesis) depended solely on the primary somatic embryo (PSE) stage of development. The PSE of the I and II class produced solely secondary somatic embryos (SSE), the III class PSE formed SSE on media containing Kin, and both SSE and adventitious buds on media containing BA, whereas the IV class PSE developed almost solely adventitious buds. BA promoted bud induction at much higher rate than Kin and slightly higher embryogenic response. The histological study confirmed these findings.
PB  - Belgrade: Institute of Forestry
C3  - Proceedings: International scientific conference sustainable use of forest ecosystems: the challenge of the 21st Century; 2006 Nov 8-10; Donji Milanovac, Serbia
T1  - Secondary regeneration in somatic embryos of Aesculus carnea Hayne
SP  - 513
EP  - 518
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6171
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Ćalić, Dušica and Budimir, Snežana and Janošević, Dušica and Vinterhalter, Branka and Radojević, Ljiljana",
year = "2006",
abstract = "Somatic embryos of red horse chestnut were subjected to an induction of secondary regeneration. The embryos were divided in 4 classes on the basis of their size (I 1, II 5, III 10 and IV 30 mm), and sub-cultivated on MS media containing 0, 1, 5 or 10 μM kinetin (Kin) or benzyladenine (BA). The pathway of secondary regeneration (somatic embryogenesis or caulogenesis) depended solely on the primary somatic embryo (PSE) stage of development. The PSE of the I and II class produced solely secondary somatic embryos (SSE), the III class PSE formed SSE on media containing Kin, and both SSE and adventitious buds on media containing BA, whereas the IV class PSE developed almost solely adventitious buds. BA promoted bud induction at much higher rate than Kin and slightly higher embryogenic response. The histological study confirmed these findings.",
publisher = "Belgrade: Institute of Forestry",
journal = "Proceedings: International scientific conference sustainable use of forest ecosystems: the challenge of the 21st Century; 2006 Nov 8-10; Donji Milanovac, Serbia",
title = "Secondary regeneration in somatic embryos of Aesculus carnea Hayne",
pages = "513-518",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6171"
}

Zdravković-Korać, S., Ćalić, D., Budimir, S., Janošević, D., Vinterhalter, B.,& Radojević, L.. (2006). Secondary regeneration in somatic embryos of Aesculus carnea Hayne. in Proceedings: International scientific conference sustainable use of forest ecosystems: the challenge of the 21st Century; 2006 Nov 8-10; Donji Milanovac, Serbia
Belgrade: Institute of Forestry., 513-518.
https://hdl.handle.net/21.15107/rcub_ibiss_6171

Zdravković-Korać S, Ćalić D, Budimir S, Janošević D, Vinterhalter B, Radojević L. Secondary regeneration in somatic embryos of Aesculus carnea Hayne. in Proceedings: International scientific conference sustainable use of forest ecosystems: the challenge of the 21st Century; 2006 Nov 8-10; Donji Milanovac, Serbia. 2006;:513-518.
https://hdl.handle.net/21.15107/rcub_ibiss_6171 .

Zdravković-Korać, Snežana, Ćalić, Dušica, Budimir, Snežana, Janošević, Dušica, Vinterhalter, Branka, Radojević, Ljiljana, "Secondary regeneration in somatic embryos of Aesculus carnea Hayne" in Proceedings: International scientific conference sustainable use of forest ecosystems: the challenge of the 21st Century; 2006 Nov 8-10; Donji Milanovac, Serbia (2006):513-518,
https://hdl.handle.net/21.15107/rcub_ibiss_6171 .