TY  - CONF
AU  - Bogdanović, Milica
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Tanić, Nikola
AU  - Mišić, Danijela
AU  - Grubišić, Dragoljub
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6170
AB  - 18S rRNA is widely used as a housekeeping gene in expression studies, including end-point PCR, Northern blotting and real-time experiments. There are, however, two disadvantages (and two points of error introduction) in using 18S rRNA as a reference gene. First, 18S has no poly-A tail, so it is traditionally reverse transcribed with either specific primers or random hexamers, e.g. separately from poly(dT)-primed transcripts. Secondly, due to its abundance, estimated to be ≈2.5 x 105 higher than an averagely expressed mRNA, the 18S cDNA must be diluted 1000-10.000 times to be comparable to the tested genes. Hereby we present that 18S rRNA can be successfully reverse transcribed using poly(dT)18 primers in all representative plant species tested: a moss, Physcomitrella patens, a fern, Adiantum capillus-veneris, a monocot, Zea mays and two dicots - Centaurium erythraea and Arabidopsis thaliana. The efficiency of the poly(dT)-primed RT was confirmed by end-point PCR and quantified by qPCR with 18S-specific primers. The efficiency of RT (miss)priming using poly(dT)18 was 90-900 times less effective than specific priming, depending on the species’ 18S sequence. Using poly(dT)18 primers for 18S RT circumvents both above mentioned obstacles, eliminating not only the need for separate RT mixtures, but also the need for 18S cDNA dilution. For theoretical rather than practical reason, all other homopolymers, poly(dA)18, poly(dC)18 and poly(dG)18, were also tested, and all were efficient as 18S RT primers. Poly(dC)18 was as efficient as specific primer, and the only one that interfered with PCR, giving species-specific pattern of products in addition to the product of expected size. The comparative results of RT-PCR and RT-qPCR of 18S rRNA with different RT primers (all homopolymers, specific primers and random hexamers) and specific PCR primers, for all five species are presented. The possible mechanism of homopolymeric (miss)priming is discussed.
PB  - European Molecular Biology Laboratory
C3  - 9th EMBL Conference on Transcription and Chromatin; 2010 Aug 28-31; Heidelberg, Germany
T1  - Homopolymeric priming of 18S rRNA in reverse transcription
SP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6170
ER  - 

@conference{
author = "Bogdanović, Milica and Dragićević, Milan and Simonović, Ana and Tanić, Nikola and Mišić, Danijela and Grubišić, Dragoljub",
year = "2010",
abstract = "18S rRNA is widely used as a housekeeping gene in expression studies, including end-point PCR, Northern blotting and real-time experiments. There are, however, two disadvantages (and two points of error introduction) in using 18S rRNA as a reference gene. First, 18S has no poly-A tail, so it is traditionally reverse transcribed with either specific primers or random hexamers, e.g. separately from poly(dT)-primed transcripts. Secondly, due to its abundance, estimated to be ≈2.5 x 105 higher than an averagely expressed mRNA, the 18S cDNA must be diluted 1000-10.000 times to be comparable to the tested genes. Hereby we present that 18S rRNA can be successfully reverse transcribed using poly(dT)18 primers in all representative plant species tested: a moss, Physcomitrella patens, a fern, Adiantum capillus-veneris, a monocot, Zea mays and two dicots - Centaurium erythraea and Arabidopsis thaliana. The efficiency of the poly(dT)-primed RT was confirmed by end-point PCR and quantified by qPCR with 18S-specific primers. The efficiency of RT (miss)priming using poly(dT)18 was 90-900 times less effective than specific priming, depending on the species’ 18S sequence. Using poly(dT)18 primers for 18S RT circumvents both above mentioned obstacles, eliminating not only the need for separate RT mixtures, but also the need for 18S cDNA dilution. For theoretical rather than practical reason, all other homopolymers, poly(dA)18, poly(dC)18 and poly(dG)18, were also tested, and all were efficient as 18S RT primers. Poly(dC)18 was as efficient as specific primer, and the only one that interfered with PCR, giving species-specific pattern of products in addition to the product of expected size. The comparative results of RT-PCR and RT-qPCR of 18S rRNA with different RT primers (all homopolymers, specific primers and random hexamers) and specific PCR primers, for all five species are presented. The possible mechanism of homopolymeric (miss)priming is discussed.",
publisher = "European Molecular Biology Laboratory",
journal = "9th EMBL Conference on Transcription and Chromatin; 2010 Aug 28-31; Heidelberg, Germany",
title = "Homopolymeric priming of 18S rRNA in reverse transcription",
pages = "108",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6170"
}

Bogdanović, M., Dragićević, M., Simonović, A., Tanić, N., Mišić, D.,& Grubišić, D.. (2010). Homopolymeric priming of 18S rRNA in reverse transcription. in 9th EMBL Conference on Transcription and Chromatin; 2010 Aug 28-31; Heidelberg, Germany
European Molecular Biology Laboratory., 108.
https://hdl.handle.net/21.15107/rcub_ibiss_6170

Bogdanović M, Dragićević M, Simonović A, Tanić N, Mišić D, Grubišić D. Homopolymeric priming of 18S rRNA in reverse transcription. in 9th EMBL Conference on Transcription and Chromatin; 2010 Aug 28-31; Heidelberg, Germany. 2010;:108.
https://hdl.handle.net/21.15107/rcub_ibiss_6170 .

Bogdanović, Milica, Dragićević, Milan, Simonović, Ana, Tanić, Nikola, Mišić, Danijela, Grubišić, Dragoljub, "Homopolymeric priming of 18S rRNA in reverse transcription" in 9th EMBL Conference on Transcription and Chromatin; 2010 Aug 28-31; Heidelberg, Germany (2010):108,
https://hdl.handle.net/21.15107/rcub_ibiss_6170 .

TY  - JOUR
AU  - Bogdanović, Milica
AU  - Sabovljević, Marko
AU  - Sabovljević, Aneta
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - https://botanicaserbica.bio.bg.ac.rs/2009_33_1.html#a488
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6175
AB  - In this paper the effects of gypsum (CaSO 4 x 2H 2 O) in the growth medium were tested on two non-gypsophilous selected bryophyte species: Bryum argenteum Hedw. and Atrichum undulatum (Hedw.) P. Beauv. With aim to test if some bryophytes are exclusive for gypsum enriched substrates, the difference in gypsum effect on moss development were examined. In vitro cultures of two selected species were initiated from nearly mature spores within unopened capsules. Half strenght Murashige and Skoog media with added gypsum in concentrations of 50, 350 and 500mM were used to test bryophyte develepment. Plants were grown on media with gypsum for 3 days or 3 weeks to compare short and long term effect of salt exposure. Tested bryophytes, non-adapted to gypsum and exposed to various gypsum concentrations in MS medium, did not show to have a problem in surviving the new environment conditions. Moss species selected in this study and exposed to gypsum enriched MS medium showed variation in morphological parameters to some extent (index of multiplication, secondary protonemal production and survival rate) and chlorophyll content and slightly altered chlorophyll a/b ratio. In general, both tested species could survive gypsum enriched medium: secondary protonema was developed and new shoots were formed in both species, but slight differences were recorded between short and long term exposure to gypsum. Acording to data obtained, gypsum is not the stressor (at least not alone) which can separate some interesting bryophytes into the ecological group of gypsophytes.
PB  - Belgrade: Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica
T1  - The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?
IS  - 1
VL  - 33
SP  - 75
EP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6175
ER  - 

@article{
author = "Bogdanović, Milica and Sabovljević, Marko and Sabovljević, Aneta and Grubišić, Dragoljub",
year = "2009",
abstract = "In this paper the effects of gypsum (CaSO 4 x 2H 2 O) in the growth medium were tested on two non-gypsophilous selected bryophyte species: Bryum argenteum Hedw. and Atrichum undulatum (Hedw.) P. Beauv. With aim to test if some bryophytes are exclusive for gypsum enriched substrates, the difference in gypsum effect on moss development were examined. In vitro cultures of two selected species were initiated from nearly mature spores within unopened capsules. Half strenght Murashige and Skoog media with added gypsum in concentrations of 50, 350 and 500mM were used to test bryophyte develepment. Plants were grown on media with gypsum for 3 days or 3 weeks to compare short and long term effect of salt exposure. Tested bryophytes, non-adapted to gypsum and exposed to various gypsum concentrations in MS medium, did not show to have a problem in surviving the new environment conditions. Moss species selected in this study and exposed to gypsum enriched MS medium showed variation in morphological parameters to some extent (index of multiplication, secondary protonemal production and survival rate) and chlorophyll content and slightly altered chlorophyll a/b ratio. In general, both tested species could survive gypsum enriched medium: secondary protonema was developed and new shoots were formed in both species, but slight differences were recorded between short and long term exposure to gypsum. Acording to data obtained, gypsum is not the stressor (at least not alone) which can separate some interesting bryophytes into the ecological group of gypsophytes.",
publisher = "Belgrade: Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica",
title = "The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?",
number = "1",
volume = "33",
pages = "75-82",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6175"
}

Bogdanović, M., Sabovljević, M., Sabovljević, A.,& Grubišić, D.. (2009). The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden Jevremovac., 33(1), 75-82.
https://hdl.handle.net/21.15107/rcub_ibiss_6175

Bogdanović M, Sabovljević M, Sabovljević A, Grubišić D. The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?. in Botanica Serbica. 2009;33(1):75-82.
https://hdl.handle.net/21.15107/rcub_ibiss_6175 .

Bogdanović, Milica, Sabovljević, Marko, Sabovljević, Aneta, Grubišić, Dragoljub, "The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?" in Botanica Serbica, 33, no. 1 (2009):75-82,
https://hdl.handle.net/21.15107/rcub_ibiss_6175 .

TY  - CONF
AU  - Bogdanović, Milica
AU  - Sabovljević, Aneta
AU  - Sabovljević, Marko
AU  - Dragićević, Milan
AU  - Platiša, Jelena
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6173
AB  - Bryophytes are thought to avoid salty substrates, although in few species reported salt tolerance is greater than in non-halophyte tracheophytes. To explore bryophyte salinity tolerance, two species were grown in vitro on half-strenght MS media with added NaCl (concentrations of 100, 250 and 500 mM). Chlorophyll content was measured spectrophotometrically, and protein content, peroxidase and catalase activities were assayed both spectrophotometrically and by PAGE. Chlorophyll content in M. polymorpha decreases rapidly with rising salt concentrations approaching zero at 250 mM, while in A. undulatum it remains high at 100 mM of salt above which decreases. Protein content remains high at 100 mM of salt in both species and decreases at higher salt concentrations (in M. polymorpha several times compared to control values). Peroxidase activity is much higher in M. polymorpha and decreases slightly at high salt concentrations. Catalase activity in A.undulatum remains high at all salt concentrations, in contrast with M. polymorpha whose activity lowers considerably at 250 mM of salt.
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia
T1  - Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L.
SP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6173
ER  - 

@conference{
author = "Bogdanović, Milica and Sabovljević, Aneta and Sabovljević, Marko and Dragićević, Milan and Platiša, Jelena and Grubišić, Dragoljub",
year = "2009",
abstract = "Bryophytes are thought to avoid salty substrates, although in few species reported salt tolerance is greater than in non-halophyte tracheophytes. To explore bryophyte salinity tolerance, two species were grown in vitro on half-strenght MS media with added NaCl (concentrations of 100, 250 and 500 mM). Chlorophyll content was measured spectrophotometrically, and protein content, peroxidase and catalase activities were assayed both spectrophotometrically and by PAGE. Chlorophyll content in M. polymorpha decreases rapidly with rising salt concentrations approaching zero at 250 mM, while in A. undulatum it remains high at 100 mM of salt above which decreases. Protein content remains high at 100 mM of salt in both species and decreases at higher salt concentrations (in M. polymorpha several times compared to control values). Peroxidase activity is much higher in M. polymorpha and decreases slightly at high salt concentrations. Catalase activity in A.undulatum remains high at all salt concentrations, in contrast with M. polymorpha whose activity lowers considerably at 250 mM of salt.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia",
title = "Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L.",
pages = "87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6173"
}

Bogdanović, M., Sabovljević, A., Sabovljević, M., Dragićević, M., Platiša, J.,& Grubišić, D.. (2009). Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L.. in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Biology., 87.
https://hdl.handle.net/21.15107/rcub_ibiss_6173

Bogdanović M, Sabovljević A, Sabovljević M, Dragićević M, Platiša J, Grubišić D. Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L.. in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia. 2009;:87.
https://hdl.handle.net/21.15107/rcub_ibiss_6173 .

Bogdanović, Milica, Sabovljević, Aneta, Sabovljević, Marko, Dragićević, Milan, Platiša, Jelena, Grubišić, Dragoljub, "Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L." in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia (2009):87,
https://hdl.handle.net/21.15107/rcub_ibiss_6173 .

TY  - CONF
AU  - Sabovljević, Marko
AU  - Sabovljević, Aneta
AU  - Vukojević, Vanja
AU  - Cvetić, Tijana
AU  - Vujičić, Milorad
AU  - Bogdanović, Milica
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6172
AB  - Bryophytes (i.e. mosses, liverworts and hornworts) are the second biggest group of the land plants. However, due to their tiny size and low economic values, they remain out of plant scientists and conservationists interest. With aim to develop better action plans for protection and conservation of these plants, we established germaplasm collection and propagation pool for 42 species of bryophytes from wide of Europe and Macaronesia. Among these species 4 are of wide European conservation interests: Anoectangium hornschuchianum (CR), Bartramia subulata (EN), Bruchia vogesiaca (VU), Ditrichum cornubicum (EN) and 6 are regionally endangered, endemic or rare species i.e. Breutelia azorica, Campylopus oerstedianus, Cyrtomnium hymenophylloides, Ditrichum plumbicola, Oreas martiana and Sphagnum palustre. Besides, counterparts of some other rare and endangered species are used as suitable model systems to test in vitro production and propagation of endangered counterparts/species: Dicranum scoparium/D. viride/D. transsylvanicum, Cinclidotus aquaticus/C. confertus, Entostodon obtusifolius/E. hungaricus, Herzogiella seligeri/H. turfacea, Plagiomnium rostratum/P.cuspidatu/P. drummondii and others. Germaplasm collection is used for active and passive species conservation and also as valuable bryophyte gene pool and reservoir for other fundamental and applied investigation.
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia
T1  - Ex situ conservation of bryophytes and bryophyte germaplasm pool
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6172
ER  - 

@conference{
author = "Sabovljević, Marko and Sabovljević, Aneta and Vukojević, Vanja and Cvetić, Tijana and Vujičić, Milorad and Bogdanović, Milica and Grubišić, Dragoljub",
year = "2009",
abstract = "Bryophytes (i.e. mosses, liverworts and hornworts) are the second biggest group of the land plants. However, due to their tiny size and low economic values, they remain out of plant scientists and conservationists interest. With aim to develop better action plans for protection and conservation of these plants, we established germaplasm collection and propagation pool for 42 species of bryophytes from wide of Europe and Macaronesia. Among these species 4 are of wide European conservation interests: Anoectangium hornschuchianum (CR), Bartramia subulata (EN), Bruchia vogesiaca (VU), Ditrichum cornubicum (EN) and 6 are regionally endangered, endemic or rare species i.e. Breutelia azorica, Campylopus oerstedianus, Cyrtomnium hymenophylloides, Ditrichum plumbicola, Oreas martiana and Sphagnum palustre. Besides, counterparts of some other rare and endangered species are used as suitable model systems to test in vitro production and propagation of endangered counterparts/species: Dicranum scoparium/D. viride/D. transsylvanicum, Cinclidotus aquaticus/C. confertus, Entostodon obtusifolius/E. hungaricus, Herzogiella seligeri/H. turfacea, Plagiomnium rostratum/P.cuspidatu/P. drummondii and others. Germaplasm collection is used for active and passive species conservation and also as valuable bryophyte gene pool and reservoir for other fundamental and applied investigation.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia",
title = "Ex situ conservation of bryophytes and bryophyte germaplasm pool",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6172"
}

Sabovljević, M., Sabovljević, A., Vukojević, V., Cvetić, T., Vujičić, M., Bogdanović, M.,& Grubišić, D.. (2009). Ex situ conservation of bryophytes and bryophyte germaplasm pool. in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Biology., 85.
https://hdl.handle.net/21.15107/rcub_ibiss_6172

Sabovljević M, Sabovljević A, Vukojević V, Cvetić T, Vujičić M, Bogdanović M, Grubišić D. Ex situ conservation of bryophytes and bryophyte germaplasm pool. in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia. 2009;:85.
https://hdl.handle.net/21.15107/rcub_ibiss_6172 .

Sabovljević, Marko, Sabovljević, Aneta, Vukojević, Vanja, Cvetić, Tijana, Vujičić, Milorad, Bogdanović, Milica, Grubišić, Dragoljub, "Ex situ conservation of bryophytes and bryophyte germaplasm pool" in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia (2009):85,
https://hdl.handle.net/21.15107/rcub_ibiss_6172 .

TY  - CONF
AU  - Bogdanović, Milica
AU  - Sabovljević, Aneta
AU  - Sabovljević, Marko
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6178
AB  - Briofite su poznate kao organizmi koji izbegavaju slane supstrate a u morima ih uopšte nema.
S ciljem izučavanja odnosa briofita prema slanoj sredini, gametofiti mahovina Atrichum undulatum, Physcomitrella patens i jetrenjače Marchantia polymorpha su gajeni na MS hranljivoj podlozi sa dodatkom različitih koncentracija NaCl (50, 100, 250, 350, 500mM) tokom 3 nedelje u uslovima dugog dana (fotoperiod 16/8h). Preživljavanje i multiplikacija izdanaka se drastično smanjuju kod sve tri vrste na visokim koncentracijama soli, a produkcija biomase kod M. polymorpha gotovo eksponencijalno opada sa povećanjem koncentracije soli. P. patens dobro preživljava na koncentracijama soli nižim od 350mM. Koncentracija hlorofila a, b i ukupnog hlorofila kod sve tri ispitivane vrste opada sa porastom koncentracije NaCl, a blagi porast količine navedenih pigmenata uočava se na podlozi obogaćenoj sa 100mM NaCl. M. polymorpha vrlo loše podnosi koncentracije soli veće od 250mM u pogledu svih ispitivanih parametara.
AB  - Bryophytes are known to avoid salty substrates and do not inhabit the seas. With the purpose of studying the relation between bryophytes and salty environment, gametophytes of the two mosses - Atrichum undulatum, Physcomitrella patens and the liverwort Marchantia polymorpha were grown on MS media enriched with various concentrations of NaCl (50, 100, 250, 350, 500mM) during three weeks at 16/8h photoperiod. Survival and bud formation were drastically reduced in all three species at high salt concentrations and biomass production in M. polymorpha declined almost exponentially as salt concentration increased. P. patens survived concentrations less than 350mM salt quite well. Chlorophyll content (-a, -b and total) in all examined species declined as salt concentration increased, however a modest rise in chlorophyll content in all three species was observed when the concentration of salt in the medium was 100mM. M. polymorpha did not tolerate well concentrations above 250mM according to all the parameters observed.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 18th Simposium Society of Plant Physiology of Serbia; 2009 May 25-27; Vršac, Serbia
T1  - Uticaj NaCl na morfogenezu i sadržaj hlorofila tri vrste briofita u kulturi in vitro
T1  - Influence of NaCl on morphogenesis and chlorophyll content of three bryophyte species in in vitro culture
SP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6178
ER  - 

@conference{
author = "Bogdanović, Milica and Sabovljević, Aneta and Sabovljević, Marko and Grubišić, Dragoljub",
year = "2009",
abstract = "Briofite su poznate kao organizmi koji izbegavaju slane supstrate a u morima ih uopšte nema.
S ciljem izučavanja odnosa briofita prema slanoj sredini, gametofiti mahovina Atrichum undulatum, Physcomitrella patens i jetrenjače Marchantia polymorpha su gajeni na MS hranljivoj podlozi sa dodatkom različitih koncentracija NaCl (50, 100, 250, 350, 500mM) tokom 3 nedelje u uslovima dugog dana (fotoperiod 16/8h). Preživljavanje i multiplikacija izdanaka se drastično smanjuju kod sve tri vrste na visokim koncentracijama soli, a produkcija biomase kod M. polymorpha gotovo eksponencijalno opada sa povećanjem koncentracije soli. P. patens dobro preživljava na koncentracijama soli nižim od 350mM. Koncentracija hlorofila a, b i ukupnog hlorofila kod sve tri ispitivane vrste opada sa porastom koncentracije NaCl, a blagi porast količine navedenih pigmenata uočava se na podlozi obogaćenoj sa 100mM NaCl. M. polymorpha vrlo loše podnosi koncentracije soli veće od 250mM u pogledu svih ispitivanih parametara., Bryophytes are known to avoid salty substrates and do not inhabit the seas. With the purpose of studying the relation between bryophytes and salty environment, gametophytes of the two mosses - Atrichum undulatum, Physcomitrella patens and the liverwort Marchantia polymorpha were grown on MS media enriched with various concentrations of NaCl (50, 100, 250, 350, 500mM) during three weeks at 16/8h photoperiod. Survival and bud formation were drastically reduced in all three species at high salt concentrations and biomass production in M. polymorpha declined almost exponentially as salt concentration increased. P. patens survived concentrations less than 350mM salt quite well. Chlorophyll content (-a, -b and total) in all examined species declined as salt concentration increased, however a modest rise in chlorophyll content in all three species was observed when the concentration of salt in the medium was 100mM. M. polymorpha did not tolerate well concentrations above 250mM according to all the parameters observed.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 18th Simposium Society of Plant Physiology of Serbia; 2009 May 25-27; Vršac, Serbia",
title = "Uticaj NaCl na morfogenezu i sadržaj hlorofila tri vrste briofita u kulturi in vitro, Influence of NaCl on morphogenesis and chlorophyll content of three bryophyte species in in vitro culture",
pages = "94",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6178"
}

Bogdanović, M., Sabovljević, A., Sabovljević, M.,& Grubišić, D.. (2009). Uticaj NaCl na morfogenezu i sadržaj hlorofila tri vrste briofita u kulturi in vitro. in Programme and Abstracts: 18th Simposium Society of Plant Physiology of Serbia; 2009 May 25-27; Vršac, Serbia
Belgrade: Serbian Plant Physiology Society., 94.
https://hdl.handle.net/21.15107/rcub_ibiss_6178

Bogdanović M, Sabovljević A, Sabovljević M, Grubišić D. Uticaj NaCl na morfogenezu i sadržaj hlorofila tri vrste briofita u kulturi in vitro. in Programme and Abstracts: 18th Simposium Society of Plant Physiology of Serbia; 2009 May 25-27; Vršac, Serbia. 2009;:94.
https://hdl.handle.net/21.15107/rcub_ibiss_6178 .

Bogdanović, Milica, Sabovljević, Aneta, Sabovljević, Marko, Grubišić, Dragoljub, "Uticaj NaCl na morfogenezu i sadržaj hlorofila tri vrste briofita u kulturi in vitro" in Programme and Abstracts: 18th Simposium Society of Plant Physiology of Serbia; 2009 May 25-27; Vršac, Serbia (2009):94,
https://hdl.handle.net/21.15107/rcub_ibiss_6178 .

TY  - JOUR
AU  - Simonović, Ana
AU  - Anderson, Marc D.
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4131
AB  - In maize roots, continuous illumination inhibits chloroplastic glutamine synthetase (GS2) activity, which decreased in light from 72.8% in 4-day-old to 26% in 10-day-old plants. In dark-adapted plants transferred to light for 6 days, GS2 activity declined from 100% to 41%, but in light-adapted plants transferred to darkness, it increased to the level of the dark control. Changes of cytosolic (GS1) activity were minor, with a similar trend. Quantitative RT-PCR revealed that light/dark treatments moderately affected only transcription of GS1 isoforms, with the exception of GS1-2, which was dramatically induced by darkness and repressed by light.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Light modulates activity and expression of glutamine synthetase isoforms in maize seedling roots
IS  - 4
VL  - 60
DO  - 10.2298/ABS0804649S
SP  - 649
EP  - 660
ER  - 

@article{
author = "Simonović, Ana and Anderson, Marc D.",
year = "2008",
abstract = "In maize roots, continuous illumination inhibits chloroplastic glutamine synthetase (GS2) activity, which decreased in light from 72.8% in 4-day-old to 26% in 10-day-old plants. In dark-adapted plants transferred to light for 6 days, GS2 activity declined from 100% to 41%, but in light-adapted plants transferred to darkness, it increased to the level of the dark control. Changes of cytosolic (GS1) activity were minor, with a similar trend. Quantitative RT-PCR revealed that light/dark treatments moderately affected only transcription of GS1 isoforms, with the exception of GS1-2, which was dramatically induced by darkness and repressed by light.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Light modulates activity and expression of glutamine synthetase isoforms in maize seedling roots",
number = "4",
volume = "60",
doi = "10.2298/ABS0804649S",
pages = "649-660"
}

Simonović, A.,& Anderson, M. D.. (2008). Light modulates activity and expression of glutamine synthetase isoforms in maize seedling roots. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 60(4), 649-660.
https://doi.org/10.2298/ABS0804649S

Simonović A, Anderson MD. Light modulates activity and expression of glutamine synthetase isoforms in maize seedling roots. in Archives of Biological Sciences. 2008;60(4):649-660.
doi:10.2298/ABS0804649S .

Simonović, Ana, Anderson, Marc D., "Light modulates activity and expression of glutamine synthetase isoforms in maize seedling roots" in Archives of Biological Sciences, 60, no. 4 (2008):649-660,
https://doi.org/10.2298/ABS0804649S . .

TY  - JOUR
AU  - Simonović, Ana
AU  - Anderson, Marc D.
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4130
AB  - Effects of chilling and acclimation on the activity of cytosolic (GS1) and plastidic (GS2) isoforms of glutamine synthetase (E.C. 6.3.1.2) were studied in chilling-sensitive and acclimation-responsive maize inbred G50. Glutamine synthetase activity in mesocotyls and roots of chilled (7 d/4ºC) and rewarmed (1 d/27ºC) etiolated plants was "1/3 that of controls. In coleoptiles+leaves of light-grown plants, GS1 was reduced to 75%, and GS2 to 50%. Acclimation (3 d/14ºC) increased GS activity and alleviated the effects of chilling. Exposure to H2O2 or menadione also reduced GS activity. Since chilling causes oxidative stress in maize, acclimation probably preserves GS activity by protecting GS from oxidative inactivation.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Effect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlings
IS  - 3
VL  - 59
DO  - 10.2298/ABS0703177S
SP  - 177
EP  - 185
ER  - 

@article{
author = "Simonović, Ana and Anderson, Marc D.",
year = "2007",
abstract = "Effects of chilling and acclimation on the activity of cytosolic (GS1) and plastidic (GS2) isoforms of glutamine synthetase (E.C. 6.3.1.2) were studied in chilling-sensitive and acclimation-responsive maize inbred G50. Glutamine synthetase activity in mesocotyls and roots of chilled (7 d/4ºC) and rewarmed (1 d/27ºC) etiolated plants was "1/3 that of controls. In coleoptiles+leaves of light-grown plants, GS1 was reduced to 75%, and GS2 to 50%. Acclimation (3 d/14ºC) increased GS activity and alleviated the effects of chilling. Exposure to H2O2 or menadione also reduced GS activity. Since chilling causes oxidative stress in maize, acclimation probably preserves GS activity by protecting GS from oxidative inactivation.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Effect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlings",
number = "3",
volume = "59",
doi = "10.2298/ABS0703177S",
pages = "177-185"
}

Simonović, A.,& Anderson, M. D.. (2007). Effect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlings. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 59(3), 177-185.
https://doi.org/10.2298/ABS0703177S

Simonović A, Anderson MD. Effect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlings. in Archives of Biological Sciences. 2007;59(3):177-185.
doi:10.2298/ABS0703177S .

Simonović, Ana, Anderson, Marc D., "Effect of chilling and acclimation on the activity of glutamine synthetase isoforms in maize seedlings" in Archives of Biological Sciences, 59, no. 3 (2007):177-185,
https://doi.org/10.2298/ABS0703177S . .