TY  - JOUR
AU  - Pantić, Igor
AU  - Jeremić, Rada
AU  - Dacić, Sanja
AU  - Peković, Sanja
AU  - Pantić, Senka
AU  - Đelić, Marina
AU  - Vitić, Zagorka
AU  - Brkić, Predrag
AU  - Brodski, Claude
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3599
AB  - Traumatic brain injury (TBI) is a main cause of death and disabilities in young adults. Although learning and memory impairments are a major clinical manifestation of TBI, the consequences of TBI on the hippocampus are still not well understood. In particular, how lesions to the sensorimotor cortex damage the hippocampus, to which it is not directly connected, is still elusive. Here, we study the effects of sensorimotor cortex ablation (SCA) on the hippocampal dentate gyrus, by applying a highly sensitive gray-level co-occurrence matrix (GLCM) analysis. Using GLCM analysis of granule neurons, we discovered, in our TBI paradigm, subtle changes in granule cell (GC) morphology, including textual uniformity, contrast, and variance, which is not detected by conventional microscopy. We conclude that sensorimotor cortex trauma leads to specific changes in the hippocampus that advance our understanding of the cellular underpinnings of cognitive impairments in TBI. Moreover, we identified GLCM analysis as a highly sensitive method to detect subtle changes in the GC layers that is expected to significantly improve further studies investigating the impact of TBI on hippocampal neuropathology.
T2  - Microscopy and Microanalysis
T1  - Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury.
IS  - 1
VL  - 26
DO  - 10.1017/S143192762000001X
SP  - 166
EP  - 172
ER  - 

@article{
author = "Pantić, Igor and Jeremić, Rada and Dacić, Sanja and Peković, Sanja and Pantić, Senka and Đelić, Marina and Vitić, Zagorka and Brkić, Predrag and Brodski, Claude",
year = "2020",
abstract = "Traumatic brain injury (TBI) is a main cause of death and disabilities in young adults. Although learning and memory impairments are a major clinical manifestation of TBI, the consequences of TBI on the hippocampus are still not well understood. In particular, how lesions to the sensorimotor cortex damage the hippocampus, to which it is not directly connected, is still elusive. Here, we study the effects of sensorimotor cortex ablation (SCA) on the hippocampal dentate gyrus, by applying a highly sensitive gray-level co-occurrence matrix (GLCM) analysis. Using GLCM analysis of granule neurons, we discovered, in our TBI paradigm, subtle changes in granule cell (GC) morphology, including textual uniformity, contrast, and variance, which is not detected by conventional microscopy. We conclude that sensorimotor cortex trauma leads to specific changes in the hippocampus that advance our understanding of the cellular underpinnings of cognitive impairments in TBI. Moreover, we identified GLCM analysis as a highly sensitive method to detect subtle changes in the GC layers that is expected to significantly improve further studies investigating the impact of TBI on hippocampal neuropathology.",
journal = "Microscopy and Microanalysis",
title = "Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury.",
number = "1",
volume = "26",
doi = "10.1017/S143192762000001X",
pages = "166-172"
}

Pantić, I., Jeremić, R., Dacić, S., Peković, S., Pantić, S., Đelić, M., Vitić, Z., Brkić, P.,& Brodski, C.. (2020). Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury.. in Microscopy and Microanalysis, 26(1), 166-172.
https://doi.org/10.1017/S143192762000001X

Pantić I, Jeremić R, Dacić S, Peković S, Pantić S, Đelić M, Vitić Z, Brkić P, Brodski C. Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury.. in Microscopy and Microanalysis. 2020;26(1):166-172.
doi:10.1017/S143192762000001X .

Pantić, Igor, Jeremić, Rada, Dacić, Sanja, Peković, Sanja, Pantić, Senka, Đelić, Marina, Vitić, Zagorka, Brkić, Predrag, Brodski, Claude, "Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury." in Microscopy and Microanalysis, 26, no. 1 (2020):166-172,
https://doi.org/10.1017/S143192762000001X . .

TY  - THES
AU  - Jakovljević, Marija
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3731
AB  - Multipla skleroza (MS) je hronična inflamacijska bolest centralnog nervnog sistema (CNS) koju kao i njen in vivo model eksperimentalni autoimunski encefalomijelitis (EAE) karakterišu infiltracija imunskih ćelija, aktivacija mikroglije i astrocita, demijelinizacija, oštećenje aksona, ali i remijelinizacija posredovana oligodendrocitnim progenitorskim ćelijama (OPĆ). Tokom neuroinflamacije, ATP ostvaruje pro-, a adenozin antiinflamacijsko dejstvo putem P2, odnosno P1 purinskih receptora. Aktivacija specifičnih purinskih receptora zavisi od koncentracije ATP, ADP i adenozina koju u vanćelijskom prostoru regulišu ektonukleotidaze. Najzastupljenije ektonukelotidaze u CNS su NTPDaza1/CD39, NTPDaza2 i eN/CD73. U MS/EAE uloga NTPDaza1/CD39 i eN/CD73 eksprimiranih na ćelijama imunskog sistema uglavnom je poznata, dok je uloga ovih enzima prisutnih na ćelijama CNS nedovoljno istražena. Budući da aktivirana mikroglija i astrociti imaju ključnu ulogu u toku neuroinflamacije glavni cilj ove disertacije bio je ispitivanje ekspresije glavnih ektonukleotidaza CNS na pomenutim ćelijama i procena njihovog inflamacijskog fenotipa, kao i ekspresije purinskih receptora u kičmenoj moždini pacova tokom EAE kao animalnog modela MS. S obzirom na ulogu OPĆ u remijelinizaciji tokom MS/EAE, dodatni cilj bio je ispitivanje uticaja proinflamacijskih faktora na vijabilnost i funkcionalnost OPĆ linije Oli-neu i ekspresiju eN/CD73 na tim ćelijama. Rezultati prikazani u ovoj disertaciji pokazali su da tokom EAE dolazi do fazno-specifičnih promena ekspresije svih ispitivanih komponenti purinskog signalnog sistema u kičmenoj moždini pacova. Uočeno povećanje ekspresije NTPDaza1/CD39 uzrokovano je aktivacijom mikroglije i infiltracijom monocita/makrofaga kao i drugih perifernih imunskih ćelija tokom EAE, a povezano je i sa tranzicijom mikroglije/makrofaga u pravcu antiinflamacijskog fenotipa, kao i indukcijom polarizacije astrocita u pravcu neuroprotektivnog fenotipa. U pogledu NTPDaza2, smanjenje ekspresije ove ektonukleotidaze prisutne prvenstveno na astrocitima u beloj masi kičmene moždine, uslovljeno je smanjenjem ekspresije na ovim ćelijama tokom EAE. Dodatno, tokom EAE došlo je do fazno-specifičnih promena u ekspresiji svih analiziranih purinskih receptora. Delovanje proinflamacijskih faktora na ćelije Oli-neu OPĆ linije izazvalo je porast ekspresije eN/CD73 koji ukazuje na inhibiciju diferencijacije i govori u prilog inhibitornoj ulozi proinflamacijskih faktora prisutnih u CNS tokom neuroinflamacije na diferencijaciju OPĆ a time i na proces remijelinizacije tokom EAE/MS, kao i na ulogu eN/CD73 ovih ćelija u tom procesu. U tom smislu, rezultati ovog istraživanja ukazuju na značajnu ulogu glavnih ektonukleotidaza CNS u toku bolesti u EAE/MS patologiji i predstavljaju osnov za razvoj novih potencijalnih terapeutika.
AB  - Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) that is characterized like its in vivo model experimental autoimmune encephalomyelitis (EAE) by immune cell infiltration, microglia and astrocyte activation, demyelination, axonal damage, as well as remyelination guided by oligodendrocyte progenitor cells (OPC). During neuroinflammation, ATP acts pro-, while adenosine acts anti-inflammatory via P2 and P1 purine receptors, respectively. Activation of specific purine receptor depends on ATP, ADP and adenosine extracellular concentrations that are regulated by by ectonucleotidases. In the CNS most abundant ectonucleotidases are NTPDase1/CD39, NTPDase2 and eN/CD73. Role of NTPDase1/CD39 and eN/CD73 in the cells of immune system, unlike in the CNS, in MS/EAE is mostly well known,. Since activated microglia and astrocytes have a key role in the course of neuroinflammation, the main goal of this dissertation was to study expression of major ectonucleotidases in the CNS at these cells and to assess their inflammatory phenotype, likewise to analyze expression of purine receptors in the rat spinal cord during EAE as animal model of MS. Considering role of OPC in remyelination during MS/EAE, additional goal was to assess the effects of proinflammatory factors at viability and functionality of OPC Oli-neu cell line and their expression of eN/CD73. Results presented herein have demonstrated disease phase-specific changes of all analyzed components of purine signaling system in rat spinal cord during EAE. Upregulation of NTPDase1/CD39 during EAE arised as a consequence of microglial activation and infiltration of monocytes/macrophages and other perypheral immune cells and also was related to transition of microglia/macrophages towards anti-inflammatory phenotype, likewise to induction of astrocyte polarization towards neuroprotective phenotype. Regarding NTPDase2, mainly expressed at white matter astrocytes, observed downregulation resulted from decreased expression by these cells during EAE. Additionally, during EAE all analyzed purine receptors showed phase-specific expression changes. Proinflammatory factors induced in OPC Oli-neu cell line upregulation of eN/CD73, indicating inhibition of differentiation, and arguing in favor of inhibitory effect of proinflammatory factors, present in the CNS during neuroinflammation, at OPC differentiation and remyelination during EAE/MS, likewise the role of eN/CD73 in that process. Thus, results presented herein indicate important role of major CNS ectonucleotidases in the disease course during EAE/MS, representing a base for development of new potential therapeutics.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Značaj signalizacije posredovane vanćelijskim purinskim nukleotidima u neuroinflamaciji i demijelinizaciji - implikacije u multiploj sklerozi
T1  - The role of purinergic signaling in neuroinflammation and demyelination – implications for multiple sclerosis
SP  - 1
EP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3731
ER  - 

@phdthesis{
author = "Jakovljević, Marija",
year = "2020",
abstract = "Multipla skleroza (MS) je hronična inflamacijska bolest centralnog nervnog sistema (CNS) koju kao i njen in vivo model eksperimentalni autoimunski encefalomijelitis (EAE) karakterišu infiltracija imunskih ćelija, aktivacija mikroglije i astrocita, demijelinizacija, oštećenje aksona, ali i remijelinizacija posredovana oligodendrocitnim progenitorskim ćelijama (OPĆ). Tokom neuroinflamacije, ATP ostvaruje pro-, a adenozin antiinflamacijsko dejstvo putem P2, odnosno P1 purinskih receptora. Aktivacija specifičnih purinskih receptora zavisi od koncentracije ATP, ADP i adenozina koju u vanćelijskom prostoru regulišu ektonukleotidaze. Najzastupljenije ektonukelotidaze u CNS su NTPDaza1/CD39, NTPDaza2 i eN/CD73. U MS/EAE uloga NTPDaza1/CD39 i eN/CD73 eksprimiranih na ćelijama imunskog sistema uglavnom je poznata, dok je uloga ovih enzima prisutnih na ćelijama CNS nedovoljno istražena. Budući da aktivirana mikroglija i astrociti imaju ključnu ulogu u toku neuroinflamacije glavni cilj ove disertacije bio je ispitivanje ekspresije glavnih ektonukleotidaza CNS na pomenutim ćelijama i procena njihovog inflamacijskog fenotipa, kao i ekspresije purinskih receptora u kičmenoj moždini pacova tokom EAE kao animalnog modela MS. S obzirom na ulogu OPĆ u remijelinizaciji tokom MS/EAE, dodatni cilj bio je ispitivanje uticaja proinflamacijskih faktora na vijabilnost i funkcionalnost OPĆ linije Oli-neu i ekspresiju eN/CD73 na tim ćelijama. Rezultati prikazani u ovoj disertaciji pokazali su da tokom EAE dolazi do fazno-specifičnih promena ekspresije svih ispitivanih komponenti purinskog signalnog sistema u kičmenoj moždini pacova. Uočeno povećanje ekspresije NTPDaza1/CD39 uzrokovano je aktivacijom mikroglije i infiltracijom monocita/makrofaga kao i drugih perifernih imunskih ćelija tokom EAE, a povezano je i sa tranzicijom mikroglije/makrofaga u pravcu antiinflamacijskog fenotipa, kao i indukcijom polarizacije astrocita u pravcu neuroprotektivnog fenotipa. U pogledu NTPDaza2, smanjenje ekspresije ove ektonukleotidaze prisutne prvenstveno na astrocitima u beloj masi kičmene moždine, uslovljeno je smanjenjem ekspresije na ovim ćelijama tokom EAE. Dodatno, tokom EAE došlo je do fazno-specifičnih promena u ekspresiji svih analiziranih purinskih receptora. Delovanje proinflamacijskih faktora na ćelije Oli-neu OPĆ linije izazvalo je porast ekspresije eN/CD73 koji ukazuje na inhibiciju diferencijacije i govori u prilog inhibitornoj ulozi proinflamacijskih faktora prisutnih u CNS tokom neuroinflamacije na diferencijaciju OPĆ a time i na proces remijelinizacije tokom EAE/MS, kao i na ulogu eN/CD73 ovih ćelija u tom procesu. U tom smislu, rezultati ovog istraživanja ukazuju na značajnu ulogu glavnih ektonukleotidaza CNS u toku bolesti u EAE/MS patologiji i predstavljaju osnov za razvoj novih potencijalnih terapeutika., Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) that is characterized like its in vivo model experimental autoimmune encephalomyelitis (EAE) by immune cell infiltration, microglia and astrocyte activation, demyelination, axonal damage, as well as remyelination guided by oligodendrocyte progenitor cells (OPC). During neuroinflammation, ATP acts pro-, while adenosine acts anti-inflammatory via P2 and P1 purine receptors, respectively. Activation of specific purine receptor depends on ATP, ADP and adenosine extracellular concentrations that are regulated by by ectonucleotidases. In the CNS most abundant ectonucleotidases are NTPDase1/CD39, NTPDase2 and eN/CD73. Role of NTPDase1/CD39 and eN/CD73 in the cells of immune system, unlike in the CNS, in MS/EAE is mostly well known,. Since activated microglia and astrocytes have a key role in the course of neuroinflammation, the main goal of this dissertation was to study expression of major ectonucleotidases in the CNS at these cells and to assess their inflammatory phenotype, likewise to analyze expression of purine receptors in the rat spinal cord during EAE as animal model of MS. Considering role of OPC in remyelination during MS/EAE, additional goal was to assess the effects of proinflammatory factors at viability and functionality of OPC Oli-neu cell line and their expression of eN/CD73. Results presented herein have demonstrated disease phase-specific changes of all analyzed components of purine signaling system in rat spinal cord during EAE. Upregulation of NTPDase1/CD39 during EAE arised as a consequence of microglial activation and infiltration of monocytes/macrophages and other perypheral immune cells and also was related to transition of microglia/macrophages towards anti-inflammatory phenotype, likewise to induction of astrocyte polarization towards neuroprotective phenotype. Regarding NTPDase2, mainly expressed at white matter astrocytes, observed downregulation resulted from decreased expression by these cells during EAE. Additionally, during EAE all analyzed purine receptors showed phase-specific expression changes. Proinflammatory factors induced in OPC Oli-neu cell line upregulation of eN/CD73, indicating inhibition of differentiation, and arguing in favor of inhibitory effect of proinflammatory factors, present in the CNS during neuroinflammation, at OPC differentiation and remyelination during EAE/MS, likewise the role of eN/CD73 in that process. Thus, results presented herein indicate important role of major CNS ectonucleotidases in the disease course during EAE/MS, representing a base for development of new potential therapeutics.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Značaj signalizacije posredovane vanćelijskim purinskim nukleotidima u neuroinflamaciji i demijelinizaciji - implikacije u multiploj sklerozi, The role of purinergic signaling in neuroinflammation and demyelination – implications for multiple sclerosis",
pages = "1-137",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3731"
}

Jakovljević, M.. (2020). Značaj signalizacije posredovane vanćelijskim purinskim nukleotidima u neuroinflamaciji i demijelinizaciji - implikacije u multiploj sklerozi. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-137.
https://hdl.handle.net/21.15107/rcub_ibiss_3731

Jakovljević M. Značaj signalizacije posredovane vanćelijskim purinskim nukleotidima u neuroinflamaciji i demijelinizaciji - implikacije u multiploj sklerozi. in University of Belgrade, Faculty of Biology. 2020;:1-137.
https://hdl.handle.net/21.15107/rcub_ibiss_3731 .

Jakovljević, Marija, "Značaj signalizacije posredovane vanćelijskim purinskim nukleotidima u neuroinflamaciji i demijelinizaciji - implikacije u multiploj sklerozi" in University of Belgrade, Faculty of Biology (2020):1-137,
https://hdl.handle.net/21.15107/rcub_ibiss_3731 .

TY  - CONF
AU  - Nikolovski, Neda
AU  - Miljković, Đorđe
AU  - Lavrnja, Irena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5987
AB  - Aims: Tolerogenic dendritic cells (tolDC) have immuno-regulatory properties and they are a promising prospective therapy for multiple sclerosis, as well as for other autoimmune diseases. Ethyl pyruvate (EP) is a redox analogue of dimethyl fumarate (Tecfidera), a drug for multiple sclerosis treatment. We have recently shown that EP has the ability to direct DC towards tolDC in both murine and human DC. Therefore, we expanded our investigation to determine which mechanisms are responsible for EPimposed tolerance in DC. Therefore, we examined Nrf2 signalling pathway, HO-1 and NQO1 enzymes, and NF-κB transcription factor. Methods: C57BL/6 mice bone marrow derived DC were cultivated for 8 days in the presence of 20 ng/mL of GM-CSF without (immature DC - iDC) or with EP added on days 3 and 6 (EP-DC). In order to induce maturation, iDC and EP-DC were incubated for 15min - 4 h with 100 ng/mL lipopolysaccharide (mature - mDC and tEP-DC, respectively). After that, immunocitochemistry staining was performed. Results: Maturation of DC led to reduction of the Nrf2 and HO-1 expression, yet this reduction was prevented by EP. Also, the NQO1 expression was higher in EP-DC in comparison to iDC. However, the expression in tEP-DC was lower than in mDC, but still higher than in iDC. Finaly, unlike mDC had higher levels of nuclear NF-κB than iDC, tEP-DC had lower expression compared to EP-DC. Conclusions: EP exercises its tolerogenic potential on DC through the up-regulation of anti-oxidative signaling pathways, as well as through the inhibition of proinflammatory transcription factor NF-κB.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Molecular Mechanisms of Ethyl Pyruvate Tolerogenic Effects on Dendritic Cells
SP  - 488
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5987
ER  - 

@conference{
author = "Nikolovski, Neda and Miljković, Đorđe and Lavrnja, Irena",
year = "2019",
abstract = "Aims: Tolerogenic dendritic cells (tolDC) have immuno-regulatory properties and they are a promising prospective therapy for multiple sclerosis, as well as for other autoimmune diseases. Ethyl pyruvate (EP) is a redox analogue of dimethyl fumarate (Tecfidera), a drug for multiple sclerosis treatment. We have recently shown that EP has the ability to direct DC towards tolDC in both murine and human DC. Therefore, we expanded our investigation to determine which mechanisms are responsible for EPimposed tolerance in DC. Therefore, we examined Nrf2 signalling pathway, HO-1 and NQO1 enzymes, and NF-κB transcription factor. Methods: C57BL/6 mice bone marrow derived DC were cultivated for 8 days in the presence of 20 ng/mL of GM-CSF without (immature DC - iDC) or with EP added on days 3 and 6 (EP-DC). In order to induce maturation, iDC and EP-DC were incubated for 15min - 4 h with 100 ng/mL lipopolysaccharide (mature - mDC and tEP-DC, respectively). After that, immunocitochemistry staining was performed. Results: Maturation of DC led to reduction of the Nrf2 and HO-1 expression, yet this reduction was prevented by EP. Also, the NQO1 expression was higher in EP-DC in comparison to iDC. However, the expression in tEP-DC was lower than in mDC, but still higher than in iDC. Finaly, unlike mDC had higher levels of nuclear NF-κB than iDC, tEP-DC had lower expression compared to EP-DC. Conclusions: EP exercises its tolerogenic potential on DC through the up-regulation of anti-oxidative signaling pathways, as well as through the inhibition of proinflammatory transcription factor NF-κB.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Molecular Mechanisms of Ethyl Pyruvate Tolerogenic Effects on Dendritic Cells",
pages = "488",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5987"
}

Nikolovski, N., Miljković, Đ.,& Lavrnja, I.. (2019). Molecular Mechanisms of Ethyl Pyruvate Tolerogenic Effects on Dendritic Cells. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 488.
https://hdl.handle.net/21.15107/rcub_ibiss_5987

Nikolovski N, Miljković Đ, Lavrnja I. Molecular Mechanisms of Ethyl Pyruvate Tolerogenic Effects on Dendritic Cells. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:488.
https://hdl.handle.net/21.15107/rcub_ibiss_5987 .

Nikolovski, Neda, Miljković, Đorđe, Lavrnja, Irena, "Molecular Mechanisms of Ethyl Pyruvate Tolerogenic Effects on Dendritic Cells" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):488,
https://hdl.handle.net/21.15107/rcub_ibiss_5987 .

TY  - CONF
AU  - Janjić, Marija
AU  - Milošević, Ana
AU  - Bjelobaba, Ivana
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5973
AB  - Two gonadotropins, luteinizing hormone and follicle-stimulating hormone, are synthetized and secreted by anterior pituitary gonadotropes and act on the gonads, controlling gametogenesis and sex hormone production. These hormones are glycoprotein polypeptides, composed of specific beta subunits and a common, alpha subunit. Both transcription and secretion of gonadotropins are regulated by gonadotropin-releasing hormone (GnRH), which is produced by small number of hypothalamic neurons within the preoptic area and mediobasal hypothalamus. GnRH is released and is reaching the pituitary in pulses, a pattern of secretion that is crucial for the proper reproductive functions. This mini review covers mechanisms of transcriptional control of gonadotropin subunit genes by GnRH, predominantly focusing on in vivo experiments with mice and rats and in vitro experiments using primary pituitary cell cultures and immortalized pituitary cell lines derived from these species. We also provide an overview of the promoter regions of gonadotropin genes and major transcription factors involved in GnRH-driven expression of gonadotropin subunit genes.
PB  - Belgrade: Faculty of Chemistry
C3  - The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia
T1  - Gonadotropin-releasing hormone regulated transcription of gonadotropin subunit genes
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5973
ER  - 

@conference{
author = "Janjić, Marija and Milošević, Ana and Bjelobaba, Ivana",
year = "2019",
abstract = "Two gonadotropins, luteinizing hormone and follicle-stimulating hormone, are synthetized and secreted by anterior pituitary gonadotropes and act on the gonads, controlling gametogenesis and sex hormone production. These hormones are glycoprotein polypeptides, composed of specific beta subunits and a common, alpha subunit. Both transcription and secretion of gonadotropins are regulated by gonadotropin-releasing hormone (GnRH), which is produced by small number of hypothalamic neurons within the preoptic area and mediobasal hypothalamus. GnRH is released and is reaching the pituitary in pulses, a pattern of secretion that is crucial for the proper reproductive functions. This mini review covers mechanisms of transcriptional control of gonadotropin subunit genes by GnRH, predominantly focusing on in vivo experiments with mice and rats and in vitro experiments using primary pituitary cell cultures and immortalized pituitary cell lines derived from these species. We also provide an overview of the promoter regions of gonadotropin genes and major transcription factors involved in GnRH-driven expression of gonadotropin subunit genes.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia",
title = "Gonadotropin-releasing hormone regulated transcription of gonadotropin subunit genes",
pages = "47",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5973"
}

Janjić, M., Milošević, A.,& Bjelobaba, I.. (2019). Gonadotropin-releasing hormone regulated transcription of gonadotropin subunit genes. in The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia
Belgrade: Faculty of Chemistry., 47.
https://hdl.handle.net/21.15107/rcub_ibiss_5973

Janjić M, Milošević A, Bjelobaba I. Gonadotropin-releasing hormone regulated transcription of gonadotropin subunit genes. in The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia. 2019;:47.
https://hdl.handle.net/21.15107/rcub_ibiss_5973 .

Janjić, Marija, Milošević, Ana, Bjelobaba, Ivana, "Gonadotropin-releasing hormone regulated transcription of gonadotropin subunit genes" in The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia (2019):47,
https://hdl.handle.net/21.15107/rcub_ibiss_5973 .

TY  - CONF
AU  - Božić, Iva
AU  - Milošević, Katarina
AU  - Janjić, Marija
AU  - Savić, Danijela
AU  - Laketa, Danijela
AU  - Jakovljević, Marija
AU  - Milošević, Ana
AU  - Peković, Sanja
AU  - Lavrnja, Irena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6006
AB  - Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS) characterized by inflammation, demyelination, neurodegeneration and gliosis. It is considered as a perplexing multifactorial disease in which the neuroendocrine system plays an important role. Growth hormone (GH) is synthesized and secreted by the somatotroph cells of the anterior pituitary. GH secretion is positively regulated by the hypothalamic factor GHRH and exerts its effects through interaction with the GH receptor (GHR), a member of the class I cytokine receptor family. It was demonstrated that neurons and astrocytes also produce GH and that GHR is widely expressed in the CNS. Nonetheless, it is not known whether expression pattern of GHR changes in the CNS during MS. We investigated GHR expression in the spinal cord during the course of experimental autoimmune encephalomyelitis (EAE), animal model of MS that is broadly used. Our results show that GHR is diminished on mRNA and protein level during EAE. Double immunofluorescence studies demonstrated that GHR is expressed in different cell types in the spinal cord in physiological conditions, including astrocytes and microglia. This expression pattern does not change extensively after the onset of EAE. However, at the peak of disease GHR is absent from astrocytes in the white and grey matter, but still present in microglia, although to a lesser degree. At the end of disease, when the animals have recovered, GHR expression is similar to control conditions. Our results point to complex involvement of GHR in the pathology of EAE.
PB  - Belgrade : Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Expression of growth hormone receptor (GHR) in experimental autoimmune encephalomyelitis
EP  - 212
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6006
ER  - 

@conference{
author = "Božić, Iva and Milošević, Katarina and Janjić, Marija and Savić, Danijela and Laketa, Danijela and Jakovljević, Marija and Milošević, Ana and Peković, Sanja and Lavrnja, Irena",
year = "2019",
abstract = "Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS) characterized by inflammation, demyelination, neurodegeneration and gliosis. It is considered as a perplexing multifactorial disease in which the neuroendocrine system plays an important role. Growth hormone (GH) is synthesized and secreted by the somatotroph cells of the anterior pituitary. GH secretion is positively regulated by the hypothalamic factor GHRH and exerts its effects through interaction with the GH receptor (GHR), a member of the class I cytokine receptor family. It was demonstrated that neurons and astrocytes also produce GH and that GHR is widely expressed in the CNS. Nonetheless, it is not known whether expression pattern of GHR changes in the CNS during MS. We investigated GHR expression in the spinal cord during the course of experimental autoimmune encephalomyelitis (EAE), animal model of MS that is broadly used. Our results show that GHR is diminished on mRNA and protein level during EAE. Double immunofluorescence studies demonstrated that GHR is expressed in different cell types in the spinal cord in physiological conditions, including astrocytes and microglia. This expression pattern does not change extensively after the onset of EAE. However, at the peak of disease GHR is absent from astrocytes in the white and grey matter, but still present in microglia, although to a lesser degree. At the end of disease, when the animals have recovered, GHR expression is similar to control conditions. Our results point to complex involvement of GHR in the pathology of EAE.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Expression of growth hormone receptor (GHR) in experimental autoimmune encephalomyelitis",
pages = "212",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6006"
}

Božić, I., Milošević, K., Janjić, M., Savić, D., Laketa, D., Jakovljević, M., Milošević, A., Peković, S.,& Lavrnja, I.. (2019). Expression of growth hormone receptor (GHR) in experimental autoimmune encephalomyelitis. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade : Serbian Neuroscience Society..
https://hdl.handle.net/21.15107/rcub_ibiss_6006

Božić I, Milošević K, Janjić M, Savić D, Laketa D, Jakovljević M, Milošević A, Peković S, Lavrnja I. Expression of growth hormone receptor (GHR) in experimental autoimmune encephalomyelitis. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:null-212.
https://hdl.handle.net/21.15107/rcub_ibiss_6006 .

Božić, Iva, Milošević, Katarina, Janjić, Marija, Savić, Danijela, Laketa, Danijela, Jakovljević, Marija, Milošević, Ana, Peković, Sanja, Lavrnja, Irena, "Expression of growth hormone receptor (GHR) in experimental autoimmune encephalomyelitis" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019),
https://hdl.handle.net/21.15107/rcub_ibiss_6006 .

TY  - CONF
AU  - Milošević, Katarina
AU  - Lavrnja, Irena
AU  - Janjić, Marija
AU  - Božić, Iva
AU  - Laketa, Danijela
AU  - Dacić, Sanja
AU  - Peković, Sanja
AU  - Savić, Danijela
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6004
AB  - Traumatic brain injury triggers neuroinflammatory response mediated by distinct populations of myeloid cells, including central nervous system (CNS) resident macrophages - microglia. Depending on the time upon insult this response may either contribute to restorative effects or hinder CNS repair.
Therefore, the focus of this study was on determining temporal course in gene expression profiles of markers specific to the mononuclear phagocyte system (MPS).
We have used the model of cortical stab injury which was performed on 3-months-old male Wistar rats. All animals were divided into 3 experimental groups: control, sham and lesion group and sacrificed at 1, 2, 3 and 7 days post-injury. After brain isolation, mRNA was extracted from cortical pieces around the center of lesion (the same tissue part was used for sham and control groups). The gene expression was analyzed by real-time PCR.
The mRNA levels of Itgam, Aif-1, Cd68 and Cx3Cr1, which are surface markers of MPS, were increased in first two days after brain injury, and then all, except Cd68, showed declining trend compared to control group. Furthermore, we analyzed expression of Arg-1, Il-6 and Tnf-alpha genes, which could be indicators of pro- or anti-inflammatory milieu. All of them increased significantly in the first two days post-injury, and then returned to control level, with the most prominent changes detected in Arg-1 mRNA level.
This study indicates enhanced MPS response in the acute phase after cortical stab injury. Further studies are required to determine which populations of CNS myeloid cells predominate in specific time point upon injury.
PB  - Belgrade : Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Mononuclear Phagocyte System In Traumatic Brain Injury
SP  - 503
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6004
ER  - 

@conference{
author = "Milošević, Katarina and Lavrnja, Irena and Janjić, Marija and Božić, Iva and Laketa, Danijela and Dacić, Sanja and Peković, Sanja and Savić, Danijela",
year = "2019",
abstract = "Traumatic brain injury triggers neuroinflammatory response mediated by distinct populations of myeloid cells, including central nervous system (CNS) resident macrophages - microglia. Depending on the time upon insult this response may either contribute to restorative effects or hinder CNS repair.
Therefore, the focus of this study was on determining temporal course in gene expression profiles of markers specific to the mononuclear phagocyte system (MPS).
We have used the model of cortical stab injury which was performed on 3-months-old male Wistar rats. All animals were divided into 3 experimental groups: control, sham and lesion group and sacrificed at 1, 2, 3 and 7 days post-injury. After brain isolation, mRNA was extracted from cortical pieces around the center of lesion (the same tissue part was used for sham and control groups). The gene expression was analyzed by real-time PCR.
The mRNA levels of Itgam, Aif-1, Cd68 and Cx3Cr1, which are surface markers of MPS, were increased in first two days after brain injury, and then all, except Cd68, showed declining trend compared to control group. Furthermore, we analyzed expression of Arg-1, Il-6 and Tnf-alpha genes, which could be indicators of pro- or anti-inflammatory milieu. All of them increased significantly in the first two days post-injury, and then returned to control level, with the most prominent changes detected in Arg-1 mRNA level.
This study indicates enhanced MPS response in the acute phase after cortical stab injury. Further studies are required to determine which populations of CNS myeloid cells predominate in specific time point upon injury.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Mononuclear Phagocyte System In Traumatic Brain Injury",
pages = "503",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6004"
}

Milošević, K., Lavrnja, I., Janjić, M., Božić, I., Laketa, D., Dacić, S., Peković, S.,& Savić, D.. (2019). Mononuclear Phagocyte System In Traumatic Brain Injury. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade : Serbian Neuroscience Society., 503.
https://hdl.handle.net/21.15107/rcub_ibiss_6004

Milošević K, Lavrnja I, Janjić M, Božić I, Laketa D, Dacić S, Peković S, Savić D. Mononuclear Phagocyte System In Traumatic Brain Injury. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:503.
https://hdl.handle.net/21.15107/rcub_ibiss_6004 .

Milošević, Katarina, Lavrnja, Irena, Janjić, Marija, Božić, Iva, Laketa, Danijela, Dacić, Sanja, Peković, Sanja, Savić, Danijela, "Mononuclear Phagocyte System In Traumatic Brain Injury" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):503,
https://hdl.handle.net/21.15107/rcub_ibiss_6004 .

TY  - JOUR
AU  - Božić, Iva
AU  - Savić, Danijela
AU  - Milošević, Ana
AU  - Janjić, Marija
AU  - Laketa, Danijela
AU  - Milošević, Katarina
AU  - Bjelobaba, Ivana
AU  - Jakovljević, Marija
AU  - Nedeljković, Nadežda
AU  - Peković, Sanja
AU  - Lavrnja, Irena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5874
AB  - Multiple sclerosis (MS) is a chronic, inflammatory, neurodegenerative disease with an autoimmune component. It was suggested that potassium channels, which are involved in crucial biological functions may have a role in different diseases, including MS and its animal model, experimental autoimmune encephalomyelitis (EAE). It was shown that voltage-gated potassium channels Kv1.5 are responsible for fine-tuning in the immune physiology and influence proliferation and differentiation in microglia and astrocytes. Here, we explored the cellular distribution of the Kv1.5 channel, together with its transcript and protein expression in the male rat spinal cord during different stages of EAE. Our results reveal a decrease of Kv1.5 transcript and protein level at the peak of disease, where massive infiltration of myeloid cells occurs, together with reactive astrogliosis and demyelination. Also, we revealed that the presence of this channel is not found in infiltrating macrophages/microglia during EAE. It is interesting to note that Kv1.5 channel is expressed only in resting microglia in the naïve animals. Predominant expression of Kv1.5 channel was found in the astrocytes in all experimental groups, while some vimentin+ cells, resembling macrophages, are devoid of Kv1.5 expression. Our results point to the possible link between Kv1.5 channel and the pathophysiological processes in EAE.
PB  - New York: Springer
T2  - Neurochemical Research
T1  - The Potassium Channel Kv1.5 Expression Alters During Experimental  Autoimmune Encephalomyelitis
IS  - 12
VL  - 44
DO  - 10.1007/s11064-019-02892-4
SP  - 2733
EP  - 2745
ER  - 

@article{
author = "Božić, Iva and Savić, Danijela and Milošević, Ana and Janjić, Marija and Laketa, Danijela and Milošević, Katarina and Bjelobaba, Ivana and Jakovljević, Marija and Nedeljković, Nadežda and Peković, Sanja and Lavrnja, Irena",
year = "2019",
abstract = "Multiple sclerosis (MS) is a chronic, inflammatory, neurodegenerative disease with an autoimmune component. It was suggested that potassium channels, which are involved in crucial biological functions may have a role in different diseases, including MS and its animal model, experimental autoimmune encephalomyelitis (EAE). It was shown that voltage-gated potassium channels Kv1.5 are responsible for fine-tuning in the immune physiology and influence proliferation and differentiation in microglia and astrocytes. Here, we explored the cellular distribution of the Kv1.5 channel, together with its transcript and protein expression in the male rat spinal cord during different stages of EAE. Our results reveal a decrease of Kv1.5 transcript and protein level at the peak of disease, where massive infiltration of myeloid cells occurs, together with reactive astrogliosis and demyelination. Also, we revealed that the presence of this channel is not found in infiltrating macrophages/microglia during EAE. It is interesting to note that Kv1.5 channel is expressed only in resting microglia in the naïve animals. Predominant expression of Kv1.5 channel was found in the astrocytes in all experimental groups, while some vimentin+ cells, resembling macrophages, are devoid of Kv1.5 expression. Our results point to the possible link between Kv1.5 channel and the pathophysiological processes in EAE.",
publisher = "New York: Springer",
journal = "Neurochemical Research",
title = "The Potassium Channel Kv1.5 Expression Alters During Experimental  Autoimmune Encephalomyelitis",
number = "12",
volume = "44",
doi = "10.1007/s11064-019-02892-4",
pages = "2733-2745"
}

Božić, I., Savić, D., Milošević, A., Janjić, M., Laketa, D., Milošević, K., Bjelobaba, I., Jakovljević, M., Nedeljković, N., Peković, S.,& Lavrnja, I.. (2019). The Potassium Channel Kv1.5 Expression Alters During Experimental  Autoimmune Encephalomyelitis. in Neurochemical Research
New York: Springer., 44(12), 2733-2745.
https://doi.org/10.1007/s11064-019-02892-4

Božić I, Savić D, Milošević A, Janjić M, Laketa D, Milošević K, Bjelobaba I, Jakovljević M, Nedeljković N, Peković S, Lavrnja I. The Potassium Channel Kv1.5 Expression Alters During Experimental  Autoimmune Encephalomyelitis. in Neurochemical Research. 2019;44(12):2733-2745.
doi:10.1007/s11064-019-02892-4 .

Božić, Iva, Savić, Danijela, Milošević, Ana, Janjić, Marija, Laketa, Danijela, Milošević, Katarina, Bjelobaba, Ivana, Jakovljević, Marija, Nedeljković, Nadežda, Peković, Sanja, Lavrnja, Irena, "The Potassium Channel Kv1.5 Expression Alters During Experimental  Autoimmune Encephalomyelitis" in Neurochemical Research, 44, no. 12 (2019):2733-2745,
https://doi.org/10.1007/s11064-019-02892-4 . .

TY  - CONF
AU  - Savić, Danijela
AU  - Opačić, Miloš
AU  - Brkljačić, Jelena
AU  - Ristić, Aleksandar
AU  - Sokić, Dragoslav
AU  - Baščarević, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Zorović, Maja
AU  - Živin, Marko
AU  - Šelih, Vid Simon
AU  - Spasić, Snežana
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5882
AB  - More and more studies are identifying the regulation of metal homeostasis as one of the key points of central nervous system’s
well-being. Epilepsy is a particularly interesting neurological condition when viewed in terms of the correlation between the
amount of metals and the development of a seizure. This lecture will present contribution of our group to the field of metal
biology in epilepsy by mapping brain metals in sclerotic hippocampus resected from drug resistant mesial temporal lobe
epilepsy (mTLE) patients as surgical therapeutic approach. Direct insight into this epileptogenic area, by two powerful techniques,
optical emission and mass spectrometry, has led us to investigation of copper turnover. Namely, among the examined metals,
we found the deficiency of copper in sclerotic hippocampus on two levels: (i) in whole structure (ii) and locally in the areas of
neuronal loss, with significant correlation between copper concentration and neuron density. Furthermore, analysis of copper
metalloproteins showed: (i) significant increase or decrease in levels of protein that is participating in copper transport into
the cell (CTR1) depending on the degree of hippocampal neuronal loss; (ii) and lower activity of an enzyme in which copper
is part of the active site, cytochrome c oxidase, in sclerotic hippocampi of patients compared to control tissue. In our further
investigations it remained to be determined whether changes in copper concentrations and copper metalloproteins are causal
to pathology of mTLE or they represent epiphenomenon.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - The importance of copper in pathology of mesial temporal lobe epilepsy
SP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5882
ER  - 

@conference{
author = "Savić, Danijela and Opačić, Miloš and Brkljačić, Jelena and Ristić, Aleksandar and Sokić, Dragoslav and Baščarević, Vladimir and Raičević, Savo and Savić, Slobodan and Zorović, Maja and Živin, Marko and Šelih, Vid Simon and Spasić, Snežana and Spasojević, Ivan",
year = "2019",
abstract = "More and more studies are identifying the regulation of metal homeostasis as one of the key points of central nervous system’s
well-being. Epilepsy is a particularly interesting neurological condition when viewed in terms of the correlation between the
amount of metals and the development of a seizure. This lecture will present contribution of our group to the field of metal
biology in epilepsy by mapping brain metals in sclerotic hippocampus resected from drug resistant mesial temporal lobe
epilepsy (mTLE) patients as surgical therapeutic approach. Direct insight into this epileptogenic area, by two powerful techniques,
optical emission and mass spectrometry, has led us to investigation of copper turnover. Namely, among the examined metals,
we found the deficiency of copper in sclerotic hippocampus on two levels: (i) in whole structure (ii) and locally in the areas of
neuronal loss, with significant correlation between copper concentration and neuron density. Furthermore, analysis of copper
metalloproteins showed: (i) significant increase or decrease in levels of protein that is participating in copper transport into
the cell (CTR1) depending on the degree of hippocampal neuronal loss; (ii) and lower activity of an enzyme in which copper
is part of the active site, cytochrome c oxidase, in sclerotic hippocampi of patients compared to control tissue. In our further
investigations it remained to be determined whether changes in copper concentrations and copper metalloproteins are causal
to pathology of mTLE or they represent epiphenomenon.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "The importance of copper in pathology of mesial temporal lobe epilepsy",
pages = "46",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5882"
}

Savić, D., Opačić, M., Brkljačić, J., Ristić, A., Sokić, D., Baščarević, V., Raičević, S., Savić, S., Zorović, M., Živin, M., Šelih, V. S., Spasić, S.,& Spasojević, I.. (2019). The importance of copper in pathology of mesial temporal lobe epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 46.
https://hdl.handle.net/21.15107/rcub_ibiss_5882

Savić D, Opačić M, Brkljačić J, Ristić A, Sokić D, Baščarević V, Raičević S, Savić S, Zorović M, Živin M, Šelih VS, Spasić S, Spasojević I. The importance of copper in pathology of mesial temporal lobe epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:46.
https://hdl.handle.net/21.15107/rcub_ibiss_5882 .

Savić, Danijela, Opačić, Miloš, Brkljačić, Jelena, Ristić, Aleksandar, Sokić, Dragoslav, Baščarević, Vladimir, Raičević, Savo, Savić, Slobodan, Zorović, Maja, Živin, Marko, Šelih, Vid Simon, Spasić, Snežana, Spasojević, Ivan, "The importance of copper in pathology of mesial temporal lobe epilepsy" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):46,
https://hdl.handle.net/21.15107/rcub_ibiss_5882 .

TY  - JOUR
AU  - Janjić, Marija
AU  - Milošević, Ana
AU  - Bjelobaba, Ivana
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5974
AB  - Two gonadotropins, luteinizing hormone and follicle-stimulating hormone, are synthetized and secreted by anterior pituitary gonadotropes and act on the gonads, controlling gametogenesis and sex hormone production. These hormones are glycoprotein polypeptides, composed of specific beta subunits and a common, alpha subunit. Both transcription and secretion of gonadotropins are regulated by gonadotropin-releasing hormone (GnRH), which is produced by a small number of hypothalamic neurons within the preoptic area and mediobasal hypothalamus. GnRH is released and reaches the pituitary in pulses, a pattern of secretion that is crucial for proper reproductive functions. This mini review covers mechanisms of transcriptional control of gonadotropin subunit genes by GnRH, predominantly focusing on in vivo experiments with mice and rats and in vitro experiments using primary pituitary cell cultures and immortalized pituitary cell lines derived from these species. We also provide an overview of the promoter regions of gonadotropin genes and major transcription factors involved in GnRH-driven expression of gonadotropin subunit genes.
PB  - Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad
T2  - Biologia Serbica
T1  - Gonadotropin-releasing hormone regulated transcription of gonadotropin subunit genes
IS  - 2
VL  - 41
DO  - 10.5281/zenodo.3532061
SP  - 51
EP  - 56
ER  - 

@article{
author = "Janjić, Marija and Milošević, Ana and Bjelobaba, Ivana",
year = "2019",
abstract = "Two gonadotropins, luteinizing hormone and follicle-stimulating hormone, are synthetized and secreted by anterior pituitary gonadotropes and act on the gonads, controlling gametogenesis and sex hormone production. These hormones are glycoprotein polypeptides, composed of specific beta subunits and a common, alpha subunit. Both transcription and secretion of gonadotropins are regulated by gonadotropin-releasing hormone (GnRH), which is produced by a small number of hypothalamic neurons within the preoptic area and mediobasal hypothalamus. GnRH is released and reaches the pituitary in pulses, a pattern of secretion that is crucial for proper reproductive functions. This mini review covers mechanisms of transcriptional control of gonadotropin subunit genes by GnRH, predominantly focusing on in vivo experiments with mice and rats and in vitro experiments using primary pituitary cell cultures and immortalized pituitary cell lines derived from these species. We also provide an overview of the promoter regions of gonadotropin genes and major transcription factors involved in GnRH-driven expression of gonadotropin subunit genes.",
publisher = "Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad",
journal = "Biologia Serbica",
title = "Gonadotropin-releasing hormone regulated transcription of gonadotropin subunit genes",
number = "2",
volume = "41",
doi = "10.5281/zenodo.3532061",
pages = "51-56"
}

Janjić, M., Milošević, A.,& Bjelobaba, I.. (2019). Gonadotropin-releasing hormone regulated transcription of gonadotropin subunit genes. in Biologia Serbica
Novi Sad: Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad., 41(2), 51-56.
https://doi.org/10.5281/zenodo.3532061

Janjić M, Milošević A, Bjelobaba I. Gonadotropin-releasing hormone regulated transcription of gonadotropin subunit genes. in Biologia Serbica. 2019;41(2):51-56.
doi:10.5281/zenodo.3532061 .

Janjić, Marija, Milošević, Ana, Bjelobaba, Ivana, "Gonadotropin-releasing hormone regulated transcription of gonadotropin subunit genes" in Biologia Serbica, 41, no. 2 (2019):51-56,
https://doi.org/10.5281/zenodo.3532061 . .

TY  - CONF
AU  - Milošević, Ana
AU  - Janjić, Marija
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Bjelobaba, Ivana
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5979
AB  - Multiple sclerosis (MS) is a chronic inflammatory neurodegenerative disease, two to three times more common in women than in men. Because the effects of neuroinflammation on the reproductive status have not been fully explored, our aim was to investigate the impact of experimental autoimmune encephalomyelitis (EAE), the rat model of MS, on hypothalamo-pituitary-gonadal axis. EAE was actively induced in Dark-Agouti rats of both sexes. The animals were examined daily for disease symptoms, weight changes, and estrous cycle phase. The animals were sacrificed at the onset, peak, and end of the disease. Hypothalamic and pituitary tissues were dissected for qRT-PCR analyses. Blood was collected for LH measurements. In separate experiments, groups of male and female animals at the peak of EAE and naïve controls received a subcutaneous injection of buserelin acetate, a potent synthetic GnRH analogue. The obtained data implied that hypothalamic neuroinflammation occurs during onset and/or peak of the disease in both sexes (upregulation of Gfap, Il1b, Il6, Ccl2 and Spp1 mRNA expression). However, hypothalamic Kiss1 and Gnrh mRNA expression was affected differently in males and females, as well as mRNA expression of pituitary signature genes - Lhb, Fshb and Gnrhr. LH levels drop transiently following the course of the disease; in females, this drop coincided with the arrest in diestrus. Nevertheless, the pituitary remained responsive to buserelin treatment. Our results indicate that EAE affects the regulation of hypothalamo-pituitary-gonadal axis in both sexes. Further analyses are needed to elucidate the causes and details of differences in hypothalamic response to neuroinflammation.
PB  - Belgrade: Faculty of Pharmacy
C3  - Abstract Book: 2nd Symposium in Biomedicine: Basic and Clinical Neuroscience; 2019 May 9; Belgrade, Serbia
T1  - Sex differences in the regulatory changes of HPG axis during experimental autoimmune encephalomyelitis in rats
SP  - 11
EP  - 12
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5979
ER  - 

@conference{
author = "Milošević, Ana and Janjić, Marija and Lavrnja, Irena and Peković, Sanja and Bjelobaba, Ivana",
year = "2019",
abstract = "Multiple sclerosis (MS) is a chronic inflammatory neurodegenerative disease, two to three times more common in women than in men. Because the effects of neuroinflammation on the reproductive status have not been fully explored, our aim was to investigate the impact of experimental autoimmune encephalomyelitis (EAE), the rat model of MS, on hypothalamo-pituitary-gonadal axis. EAE was actively induced in Dark-Agouti rats of both sexes. The animals were examined daily for disease symptoms, weight changes, and estrous cycle phase. The animals were sacrificed at the onset, peak, and end of the disease. Hypothalamic and pituitary tissues were dissected for qRT-PCR analyses. Blood was collected for LH measurements. In separate experiments, groups of male and female animals at the peak of EAE and naïve controls received a subcutaneous injection of buserelin acetate, a potent synthetic GnRH analogue. The obtained data implied that hypothalamic neuroinflammation occurs during onset and/or peak of the disease in both sexes (upregulation of Gfap, Il1b, Il6, Ccl2 and Spp1 mRNA expression). However, hypothalamic Kiss1 and Gnrh mRNA expression was affected differently in males and females, as well as mRNA expression of pituitary signature genes - Lhb, Fshb and Gnrhr. LH levels drop transiently following the course of the disease; in females, this drop coincided with the arrest in diestrus. Nevertheless, the pituitary remained responsive to buserelin treatment. Our results indicate that EAE affects the regulation of hypothalamo-pituitary-gonadal axis in both sexes. Further analyses are needed to elucidate the causes and details of differences in hypothalamic response to neuroinflammation.",
publisher = "Belgrade: Faculty of Pharmacy",
journal = "Abstract Book: 2nd Symposium in Biomedicine: Basic and Clinical Neuroscience; 2019 May 9; Belgrade, Serbia",
title = "Sex differences in the regulatory changes of HPG axis during experimental autoimmune encephalomyelitis in rats",
pages = "11-12",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5979"
}

Milošević, A., Janjić, M., Lavrnja, I., Peković, S.,& Bjelobaba, I.. (2019). Sex differences in the regulatory changes of HPG axis during experimental autoimmune encephalomyelitis in rats. in Abstract Book: 2nd Symposium in Biomedicine: Basic and Clinical Neuroscience; 2019 May 9; Belgrade, Serbia
Belgrade: Faculty of Pharmacy., 11-12.
https://hdl.handle.net/21.15107/rcub_ibiss_5979

Milošević A, Janjić M, Lavrnja I, Peković S, Bjelobaba I. Sex differences in the regulatory changes of HPG axis during experimental autoimmune encephalomyelitis in rats. in Abstract Book: 2nd Symposium in Biomedicine: Basic and Clinical Neuroscience; 2019 May 9; Belgrade, Serbia. 2019;:11-12.
https://hdl.handle.net/21.15107/rcub_ibiss_5979 .

Milošević, Ana, Janjić, Marija, Lavrnja, Irena, Peković, Sanja, Bjelobaba, Ivana, "Sex differences in the regulatory changes of HPG axis during experimental autoimmune encephalomyelitis in rats" in Abstract Book: 2nd Symposium in Biomedicine: Basic and Clinical Neuroscience; 2019 May 9; Belgrade, Serbia (2019):11-12,
https://hdl.handle.net/21.15107/rcub_ibiss_5979 .

TY  - CONF
AU  - Jakovljević, Marija
AU  - Lavrnja, Irena
AU  - Božić, Iva
AU  - Milošević, Ana
AU  - Bjelobaba, Ivana
AU  - Savić, Danijela
AU  - Peković, Sanja
AU  - Nedeljković, Nadežda
AU  - Laketa, Danijela
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5982
AB  - Considering neuroinflammatory paradigm, increased extracellular levels of ATP have adverse effects, while adenosine is predominantly anti-inflammatory. In the CNS, NTPDase1/CD39 is the main enzyme that initiates the degradation pathway of extracellular ATP to adenosine. The aim of the study was to explore the activation state of the cells that express NTPDase1/CD39 – microglia and macrophages, during experimental autoimmune encephalomyelitis (EAE). Acute monophasic EAE was induced in female Dark Agouti rats. Animals were sacrificed at the disease onset (Eo), peak (Ep) and end (Ee). The lumbosacral parts of spinal cords were isolated for gene (qRT-PCR and in situ hybridization) and protein expression analysis (Western Blot, immunofluorescence and flow cytometry). Activation state of microglia/macrophages was assessed by colocalization analysis of NTPDase1/Iba1 and NTPDase1/CD68 with iNOS or Arg1 as specific markers of pro- and antiinflammatory state, respectively. During EAE, NTPDase1/CD39 was significantly increased both at mRNA and protein level at Ep. Immunofluorescence combined with flow cytometry showed that reactive microglia and mononuclear infiltrates accounted for the most of the observed increase. Both Iba1 and CD68 microglia/macrophage markers showed higher co-occurrence with iNOS at Eo and Arg1 at Ep, suggesting prevalence of M1-like at Eo and M2-like at Ep. In addition, NTPDase1 showed about three-times higher colocalization with Arg1 compared to iNOS at Ep, suggesting its higher association with M2-like activation state of microglia/ macrophages. Together, our data suggest an association between NTPDase1 up-regulation by reactive microglia and infiltrated macrophages and their transition toward anti-inflammatory phenotype in EAE.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - NTPDase1/CD39 Expression Increased During EAE in Association with Number and Activation State of Microglia/Macrophages
SP  - 492
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5982
ER  - 

@conference{
author = "Jakovljević, Marija and Lavrnja, Irena and Božić, Iva and Milošević, Ana and Bjelobaba, Ivana and Savić, Danijela and Peković, Sanja and Nedeljković, Nadežda and Laketa, Danijela",
year = "2019",
abstract = "Considering neuroinflammatory paradigm, increased extracellular levels of ATP have adverse effects, while adenosine is predominantly anti-inflammatory. In the CNS, NTPDase1/CD39 is the main enzyme that initiates the degradation pathway of extracellular ATP to adenosine. The aim of the study was to explore the activation state of the cells that express NTPDase1/CD39 – microglia and macrophages, during experimental autoimmune encephalomyelitis (EAE). Acute monophasic EAE was induced in female Dark Agouti rats. Animals were sacrificed at the disease onset (Eo), peak (Ep) and end (Ee). The lumbosacral parts of spinal cords were isolated for gene (qRT-PCR and in situ hybridization) and protein expression analysis (Western Blot, immunofluorescence and flow cytometry). Activation state of microglia/macrophages was assessed by colocalization analysis of NTPDase1/Iba1 and NTPDase1/CD68 with iNOS or Arg1 as specific markers of pro- and antiinflammatory state, respectively. During EAE, NTPDase1/CD39 was significantly increased both at mRNA and protein level at Ep. Immunofluorescence combined with flow cytometry showed that reactive microglia and mononuclear infiltrates accounted for the most of the observed increase. Both Iba1 and CD68 microglia/macrophage markers showed higher co-occurrence with iNOS at Eo and Arg1 at Ep, suggesting prevalence of M1-like at Eo and M2-like at Ep. In addition, NTPDase1 showed about three-times higher colocalization with Arg1 compared to iNOS at Ep, suggesting its higher association with M2-like activation state of microglia/ macrophages. Together, our data suggest an association between NTPDase1 up-regulation by reactive microglia and infiltrated macrophages and their transition toward anti-inflammatory phenotype in EAE.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "NTPDase1/CD39 Expression Increased During EAE in Association with Number and Activation State of Microglia/Macrophages",
pages = "492",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5982"
}

Jakovljević, M., Lavrnja, I., Božić, I., Milošević, A., Bjelobaba, I., Savić, D., Peković, S., Nedeljković, N.,& Laketa, D.. (2019). NTPDase1/CD39 Expression Increased During EAE in Association with Number and Activation State of Microglia/Macrophages. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 492.
https://hdl.handle.net/21.15107/rcub_ibiss_5982

Jakovljević M, Lavrnja I, Božić I, Milošević A, Bjelobaba I, Savić D, Peković S, Nedeljković N, Laketa D. NTPDase1/CD39 Expression Increased During EAE in Association with Number and Activation State of Microglia/Macrophages. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:492.
https://hdl.handle.net/21.15107/rcub_ibiss_5982 .

Jakovljević, Marija, Lavrnja, Irena, Božić, Iva, Milošević, Ana, Bjelobaba, Ivana, Savić, Danijela, Peković, Sanja, Nedeljković, Nadežda, Laketa, Danijela, "NTPDase1/CD39 Expression Increased During EAE in Association with Number and Activation State of Microglia/Macrophages" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):492,
https://hdl.handle.net/21.15107/rcub_ibiss_5982 .

TY  - CONF
AU  - Opačić, Miloš
AU  - Zorović, Maja
AU  - Savić, Danijela
AU  - Živin, Marko
AU  - Raičević, Savo
AU  - Baščarević, Vladimir
AU  - Ristić, Aleksandar
AU  - Sokić, Dragoslav
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5986
AB  - Aims: A drop in copper level and the loss of energy homeostasis are both portrayed in mesial temporal lobe epilepsy (mTLE) with hippocampal sclerosis (HS) patients. Cytochrome c oxidase (COX) represents a crossroad of energy and copper metabolism; it is a key component of mitochondrial machinery and contains two copper centers. Our aim here was to examine the link between COX activity and the copper transporting system in HS. COX activity and the levels of mRNA of selected chaperones - COX11, COX17, Sco1 and Sco2 were determined in 13 anatomically distinct hippocampal regions. Methods: Study was performed on seven hippocampal samples, four of which had been acquired during the course of amygdalohippocampectomy treatment of medically intractable epilepsy and three control postmortem samples. Adjacent slices were used for Nissl staining, COX activity assay and mRNA in situ hybridization with autoradiography. Densitometry was performed using ImageJ. Results: Overall COX activity was decreased in HS compared to controls (P = 0.0003). However, 5 regions showed significantly lower COX activity in HS and 8 did not. Subiculum showed slightly higher activity in HS. The levels of mRNA levels were lowered in HS in 6 regions for COX11, 10 regions for COX17, two regions for Sco1 and 11 regions for Sco2. Conclusions: Our findings suggest the loss of energy homeostasis in HS may be related to pathological changes in specific components of copper delivery to COX, and that the impact may vary between different hippocampal regions.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients
SP  - 296
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5986
ER  - 

@conference{
author = "Opačić, Miloš and Zorović, Maja and Savić, Danijela and Živin, Marko and Raičević, Savo and Baščarević, Vladimir and Ristić, Aleksandar and Sokić, Dragoslav and Spasojević, Ivan",
year = "2019",
abstract = "Aims: A drop in copper level and the loss of energy homeostasis are both portrayed in mesial temporal lobe epilepsy (mTLE) with hippocampal sclerosis (HS) patients. Cytochrome c oxidase (COX) represents a crossroad of energy and copper metabolism; it is a key component of mitochondrial machinery and contains two copper centers. Our aim here was to examine the link between COX activity and the copper transporting system in HS. COX activity and the levels of mRNA of selected chaperones - COX11, COX17, Sco1 and Sco2 were determined in 13 anatomically distinct hippocampal regions. Methods: Study was performed on seven hippocampal samples, four of which had been acquired during the course of amygdalohippocampectomy treatment of medically intractable epilepsy and three control postmortem samples. Adjacent slices were used for Nissl staining, COX activity assay and mRNA in situ hybridization with autoradiography. Densitometry was performed using ImageJ. Results: Overall COX activity was decreased in HS compared to controls (P = 0.0003). However, 5 regions showed significantly lower COX activity in HS and 8 did not. Subiculum showed slightly higher activity in HS. The levels of mRNA levels were lowered in HS in 6 regions for COX11, 10 regions for COX17, two regions for Sco1 and 11 regions for Sco2. Conclusions: Our findings suggest the loss of energy homeostasis in HS may be related to pathological changes in specific components of copper delivery to COX, and that the impact may vary between different hippocampal regions.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients",
pages = "296",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5986"
}

Opačić, M., Zorović, M., Savić, D., Živin, M., Raičević, S., Baščarević, V., Ristić, A., Sokić, D.,& Spasojević, I.. (2019). Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 296.
https://hdl.handle.net/21.15107/rcub_ibiss_5986

Opačić M, Zorović M, Savić D, Živin M, Raičević S, Baščarević V, Ristić A, Sokić D, Spasojević I. Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:296.
https://hdl.handle.net/21.15107/rcub_ibiss_5986 .

Opačić, Miloš, Zorović, Maja, Savić, Danijela, Živin, Marko, Raičević, Savo, Baščarević, Vladimir, Ristić, Aleksandar, Sokić, Dragoslav, Spasojević, Ivan, "Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):296,
https://hdl.handle.net/21.15107/rcub_ibiss_5986 .

TY  - CONF
AU  - Janjić, Marija
AU  - Previde, Rafael Maso
AU  - Smiljanić, Kosara
AU  - Fletcher, Patrick A.
AU  - Sherman, Arthur
AU  - Bjelobaba, Ivana
AU  - Stojilkovic, Stanko S.
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5981
AB  - Aim: To investigate mechanisms of inhibition of reproductive functions by continuous GnRH application, with focus on gonadotropin gene expression and secretion. Methods: Experiments were performed in vivo and in vitro using female and male rats and cultured pituitary cells. Gene expression was characterized by qRT-PCR analysis. For this purpose, we searched for an appropriate reference gene. Protein expression was characterized by immunocytochemistry, and ELISA and Western blot analyses were used for LH measurements. Results: Continuous exposure of pituitary cells in static cultures to Gnrh agonists induced a prolonged blockade of Fshb expression after a brief stimulation. However, only a minor and transient inhibitory effect on Lhb expression was detected. Such Lhb profile probably reflects the expression status of three genes controlling Lhb transcription during the treatment: stimulation of Egr1, inhibition of Nr5a1, and no effect on Pitx1 expression. In contrast, continuous Gnrh treatment stimulated Lh secretion in static cultures, leading to depletion of the secretory pool. In vivo administration of a Gnrh agonist was also accompanied with a rapid increase in serum Lh levels and a progressive depletion of the intrapituitary Lh levels without major effects on Lhb expression. Conclusion: Blockade of Fshb expression and depletion of the Lh secretory pool are two major factors accounting for weakening of the gonadotroph secretory function during continuous Gnrh treatment.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Continuous GnRH Treatment Blocks Basal Fshb but not Lhb Expression in Rat Pituitary Gonadotrophs
SP  - 367
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5981
ER  - 

@conference{
author = "Janjić, Marija and Previde, Rafael Maso and Smiljanić, Kosara and Fletcher, Patrick A. and Sherman, Arthur and Bjelobaba, Ivana and Stojilkovic, Stanko S.",
year = "2019",
abstract = "Aim: To investigate mechanisms of inhibition of reproductive functions by continuous GnRH application, with focus on gonadotropin gene expression and secretion. Methods: Experiments were performed in vivo and in vitro using female and male rats and cultured pituitary cells. Gene expression was characterized by qRT-PCR analysis. For this purpose, we searched for an appropriate reference gene. Protein expression was characterized by immunocytochemistry, and ELISA and Western blot analyses were used for LH measurements. Results: Continuous exposure of pituitary cells in static cultures to Gnrh agonists induced a prolonged blockade of Fshb expression after a brief stimulation. However, only a minor and transient inhibitory effect on Lhb expression was detected. Such Lhb profile probably reflects the expression status of three genes controlling Lhb transcription during the treatment: stimulation of Egr1, inhibition of Nr5a1, and no effect on Pitx1 expression. In contrast, continuous Gnrh treatment stimulated Lh secretion in static cultures, leading to depletion of the secretory pool. In vivo administration of a Gnrh agonist was also accompanied with a rapid increase in serum Lh levels and a progressive depletion of the intrapituitary Lh levels without major effects on Lhb expression. Conclusion: Blockade of Fshb expression and depletion of the Lh secretory pool are two major factors accounting for weakening of the gonadotroph secretory function during continuous Gnrh treatment.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Continuous GnRH Treatment Blocks Basal Fshb but not Lhb Expression in Rat Pituitary Gonadotrophs",
pages = "367",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5981"
}

Janjić, M., Previde, R. M., Smiljanić, K., Fletcher, P. A., Sherman, A., Bjelobaba, I.,& Stojilkovic, S. S.. (2019). Continuous GnRH Treatment Blocks Basal Fshb but not Lhb Expression in Rat Pituitary Gonadotrophs. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 367.
https://hdl.handle.net/21.15107/rcub_ibiss_5981

Janjić M, Previde RM, Smiljanić K, Fletcher PA, Sherman A, Bjelobaba I, Stojilkovic SS. Continuous GnRH Treatment Blocks Basal Fshb but not Lhb Expression in Rat Pituitary Gonadotrophs. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:367.
https://hdl.handle.net/21.15107/rcub_ibiss_5981 .

Janjić, Marija, Previde, Rafael Maso, Smiljanić, Kosara, Fletcher, Patrick A., Sherman, Arthur, Bjelobaba, Ivana, Stojilkovic, Stanko S., "Continuous GnRH Treatment Blocks Basal Fshb but not Lhb Expression in Rat Pituitary Gonadotrophs" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):367,
https://hdl.handle.net/21.15107/rcub_ibiss_5981 .

TY  - JOUR
AU  - Dacić, Sanja
AU  - Božić, Iva
AU  - Jeremić, Rada
AU  - Bjelobaba, Ivana
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana
AU  - Peković, Sanja
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5989
AB  - Aims: Traumatic brain injury (TBI) causes disruption in homeostasis of calcium ions (Ca2+), important second messenger considered as the major culprit of secondary injury and TBI-induced neuronal damage and death. Ca2+ entry into the cells occurs via various types of voltage-dependent calcium channels (VDCCs). The aim of this study was to evaluate the involvement of Ca2+ entry via L-type CaV1.2 VDCCs in the processes of neuroinflammation and regeneration after brain injury. Methods: TBI was performed on male Wistar rats by sensorimotor cortex ablation (SCA) at the following coordinates: 2 mm anterior and 4 mm posterior to bregma, and 4 mm lateral from the midline. Temporal and cellular pattern of CaV1.2 expression was followed at different time points post-injury (2, 7, 14, 30 dpi) using double immunofluorescence staining with specific markers. Results: Upregulation of CaV1.2 expression was detected on reactive astrocytes and astrocytic processes that form glial scar around the lesion site, on subset of proinflammatory microglia/macrophages and neutrophils surrounding the lesion cavity. Interestingly, presence of CaV1.2+ cells was detected in the migratory pathway, consisted of DCX+ progenitors, extending from subventricular zone up to the lesion site. Furthermore, CaV1.2+/DCX+ newborn neurons were detected in subgranular layer of hippocampal dentate gyrus. Conclusions: We concluded that L-type CaV1.2 calcium channel has an important role in the regulation of processes of neuroinflammation, neuroregeneration and neurogenesis, pointing to the complexity of intercellular regulation of Ca2+ homeostasis after brain injury. Consequently, modulation of CaV1.2 channels expression may be potential target for the treatment of brain injury.
PB  - Belgrade: Serbian Neuroscience Society
T2  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - L-type Calcium Channels Involvement in the Regulation of Neuroinflammation and Neuroregeneration After Brain Injury
SP  - 487
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5989
ER  - 

@article{
author = "Dacić, Sanja and Božić, Iva and Jeremić, Rada and Bjelobaba, Ivana and Lavrnja, Irena and Savić, Danijela and Rakić, Ljubisav and Stojiljković, Mirjana and Peković, Sanja",
year = "2019",
abstract = "Aims: Traumatic brain injury (TBI) causes disruption in homeostasis of calcium ions (Ca2+), important second messenger considered as the major culprit of secondary injury and TBI-induced neuronal damage and death. Ca2+ entry into the cells occurs via various types of voltage-dependent calcium channels (VDCCs). The aim of this study was to evaluate the involvement of Ca2+ entry via L-type CaV1.2 VDCCs in the processes of neuroinflammation and regeneration after brain injury. Methods: TBI was performed on male Wistar rats by sensorimotor cortex ablation (SCA) at the following coordinates: 2 mm anterior and 4 mm posterior to bregma, and 4 mm lateral from the midline. Temporal and cellular pattern of CaV1.2 expression was followed at different time points post-injury (2, 7, 14, 30 dpi) using double immunofluorescence staining with specific markers. Results: Upregulation of CaV1.2 expression was detected on reactive astrocytes and astrocytic processes that form glial scar around the lesion site, on subset of proinflammatory microglia/macrophages and neutrophils surrounding the lesion cavity. Interestingly, presence of CaV1.2+ cells was detected in the migratory pathway, consisted of DCX+ progenitors, extending from subventricular zone up to the lesion site. Furthermore, CaV1.2+/DCX+ newborn neurons were detected in subgranular layer of hippocampal dentate gyrus. Conclusions: We concluded that L-type CaV1.2 calcium channel has an important role in the regulation of processes of neuroinflammation, neuroregeneration and neurogenesis, pointing to the complexity of intercellular regulation of Ca2+ homeostasis after brain injury. Consequently, modulation of CaV1.2 channels expression may be potential target for the treatment of brain injury.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "L-type Calcium Channels Involvement in the Regulation of Neuroinflammation and Neuroregeneration After Brain Injury",
pages = "487",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5989"
}

Dacić, S., Božić, I., Jeremić, R., Bjelobaba, I., Lavrnja, I., Savić, D., Rakić, L., Stojiljković, M.,& Peković, S.. (2019). L-type Calcium Channels Involvement in the Regulation of Neuroinflammation and Neuroregeneration After Brain Injury. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 487.
https://hdl.handle.net/21.15107/rcub_ibiss_5989

Dacić S, Božić I, Jeremić R, Bjelobaba I, Lavrnja I, Savić D, Rakić L, Stojiljković M, Peković S. L-type Calcium Channels Involvement in the Regulation of Neuroinflammation and Neuroregeneration After Brain Injury. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:487.
https://hdl.handle.net/21.15107/rcub_ibiss_5989 .

Dacić, Sanja, Božić, Iva, Jeremić, Rada, Bjelobaba, Ivana, Lavrnja, Irena, Savić, Danijela, Rakić, Ljubisav, Stojiljković, Mirjana, Peković, Sanja, "L-type Calcium Channels Involvement in the Regulation of Neuroinflammation and Neuroregeneration After Brain Injury" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):487,
https://hdl.handle.net/21.15107/rcub_ibiss_5989 .

TY  - CONF
AU  - Ristić, Aleksandar
AU  - Savić, Danijela
AU  - Bogdanović Pristov, Jelena
AU  - Brkljačić, Jelena
AU  - Baščarević, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5991
AB  - Mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS) is probably the single most frequent human focal epilepsy. The involvement of redox processes in the pathological mechanisms of mTLE-HS has been implicated  by mitochondrial dysfunction and oxidative damage, and by different metabolic abnormalities that have been observed in sclerotic hippocampi, such as altered metabolism of redox-active metals. The strongest proof came with the analysis of enzymatic antioxidative system. Sclerotic hippocampi show drastically increased activity and levels of hydrogen peroxide–removing enzymes – catalase and glutathione peroxidase/reductase. Catalase is located mainly in neurons in both, controls and HS. Sclerotic hippocampi are depleted of glutathione peroxidase-positive blood vessels that are present in control hippocampi. Pertinent to this, it has been documented that hippocampi of mTLE-HS patients show increased blood vessel density, but most of the vessels represent atrophic vascular structures. On the other hand, HS showes specific glutathione peroxidase-rich loci that are present in gyrus dentatus, CA regions, and alveus, and appear to represent bundles of astrocytes. These loci are probably sites of excessive (neuronal) production of hydrogen peroxide that is counteracted by astrocytes. Finally, protein levels of mitochondrial enzyme manganese superoxide dismutase are higher in HS than controls. Neurons with abnormal morphology and strong superoxide dismutase immunofluorescence are present in all neuronal layers in HS. In close, antioxidative system is upregulated in HS implying that epileptogenic hippocampi are exposed to oxidative stress. The involvement of redox alterations in the pathology of epilepsy may open new pharmacologic perspectives for mTLE-HS treatment.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Hippocampal Antioxidative System in Epilepsy
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5991
ER  - 

@conference{
author = "Ristić, Aleksandar and Savić, Danijela and Bogdanović Pristov, Jelena and Brkljačić, Jelena and Baščarević, Vladimir and Raičević, Savo and Savić, Slobodan",
year = "2019",
abstract = "Mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS) is probably the single most frequent human focal epilepsy. The involvement of redox processes in the pathological mechanisms of mTLE-HS has been implicated  by mitochondrial dysfunction and oxidative damage, and by different metabolic abnormalities that have been observed in sclerotic hippocampi, such as altered metabolism of redox-active metals. The strongest proof came with the analysis of enzymatic antioxidative system. Sclerotic hippocampi show drastically increased activity and levels of hydrogen peroxide–removing enzymes – catalase and glutathione peroxidase/reductase. Catalase is located mainly in neurons in both, controls and HS. Sclerotic hippocampi are depleted of glutathione peroxidase-positive blood vessels that are present in control hippocampi. Pertinent to this, it has been documented that hippocampi of mTLE-HS patients show increased blood vessel density, but most of the vessels represent atrophic vascular structures. On the other hand, HS showes specific glutathione peroxidase-rich loci that are present in gyrus dentatus, CA regions, and alveus, and appear to represent bundles of astrocytes. These loci are probably sites of excessive (neuronal) production of hydrogen peroxide that is counteracted by astrocytes. Finally, protein levels of mitochondrial enzyme manganese superoxide dismutase are higher in HS than controls. Neurons with abnormal morphology and strong superoxide dismutase immunofluorescence are present in all neuronal layers in HS. In close, antioxidative system is upregulated in HS implying that epileptogenic hippocampi are exposed to oxidative stress. The involvement of redox alterations in the pathology of epilepsy may open new pharmacologic perspectives for mTLE-HS treatment.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Hippocampal Antioxidative System in Epilepsy",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5991"
}

Ristić, A., Savić, D., Bogdanović Pristov, J., Brkljačić, J., Baščarević, V., Raičević, S.,& Savić, S.. (2019). Hippocampal Antioxidative System in Epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 42.
https://hdl.handle.net/21.15107/rcub_ibiss_5991

Ristić A, Savić D, Bogdanović Pristov J, Brkljačić J, Baščarević V, Raičević S, Savić S. Hippocampal Antioxidative System in Epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:42.
https://hdl.handle.net/21.15107/rcub_ibiss_5991 .

Ristić, Aleksandar, Savić, Danijela, Bogdanović Pristov, Jelena, Brkljačić, Jelena, Baščarević, Vladimir, Raičević, Savo, Savić, Slobodan, "Hippocampal Antioxidative System in Epilepsy" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):42,
https://hdl.handle.net/21.15107/rcub_ibiss_5991 .

TY  - CONF
AU  - Milošević, Ana
AU  - Janjić, Marija
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Jakovljević, Marija
AU  - Peković, Sanja
AU  - Bjelobaba, Ivana
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5972
AB  - Aims: Multiple sclerosis (MS) is a chronic neuroinflammatory disease, more common in women than in men. Because the effects of MS on hypothalamo-pituitary-gonadal axis haven’t been completely elucidated, our aim was to investigate the impact of experimental autoimmune encephalomyelitis (EAE) on reproductive functions in rats. Methods: EAE was actively induced in Dark-Agouti rats of both sexes. Disease symptoms, weight changes, and estrous cycle phase were assessed daily. The animals were sacrificed at the onset, peak, and end of EAE. Hypothalamic, pituitary and gonadal tissues were dissected for qRT-PCR and/or protein extraction. Blood was collected for hormone measurements. In separate experiments, animals at the peak of EAE and naïve controls received an injection of a GnRH analogue - buserelin. Results: Our results suggest hypothalamic neuroinflammation in both sexes; upregulation of mRNA for several genes was registered during EAE. Hypothalamic expression of Kiss1 and Gnrh, as well as pituitary expression of Lhb, Fshb and Gnrhr mRNA, were affected differently in males and females. LH levels drop transiently following the course of EAE, coinciding with the arrest in diestrus in females and a drop in testosterone levels in males. Buserelin increased LH levels in both sexes. Additionally, StAR – a protein with a critical role in steroid hormone biosynthesis, had an opposite pattern of expression in ovaries and testicular interstitial cells during the disease, both on mRNA and protein level. Conclusion: Our data indicate that EAE noticeably affects the regulation of HPG axis. Further analyses are needed to explore the details of this phenomenon.
PB  - Belgrade : Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Experimental Autoimmune Encephalomyelitis Disturbs the Regulation of HPG Axis in Rats of Both Sexes
SP  - 293
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5972
ER  - 

@conference{
author = "Milošević, Ana and Janjić, Marija and Lavrnja, Irena and Savić, Danijela and Jakovljević, Marija and Peković, Sanja and Bjelobaba, Ivana",
year = "2019",
abstract = "Aims: Multiple sclerosis (MS) is a chronic neuroinflammatory disease, more common in women than in men. Because the effects of MS on hypothalamo-pituitary-gonadal axis haven’t been completely elucidated, our aim was to investigate the impact of experimental autoimmune encephalomyelitis (EAE) on reproductive functions in rats. Methods: EAE was actively induced in Dark-Agouti rats of both sexes. Disease symptoms, weight changes, and estrous cycle phase were assessed daily. The animals were sacrificed at the onset, peak, and end of EAE. Hypothalamic, pituitary and gonadal tissues were dissected for qRT-PCR and/or protein extraction. Blood was collected for hormone measurements. In separate experiments, animals at the peak of EAE and naïve controls received an injection of a GnRH analogue - buserelin. Results: Our results suggest hypothalamic neuroinflammation in both sexes; upregulation of mRNA for several genes was registered during EAE. Hypothalamic expression of Kiss1 and Gnrh, as well as pituitary expression of Lhb, Fshb and Gnrhr mRNA, were affected differently in males and females. LH levels drop transiently following the course of EAE, coinciding with the arrest in diestrus in females and a drop in testosterone levels in males. Buserelin increased LH levels in both sexes. Additionally, StAR – a protein with a critical role in steroid hormone biosynthesis, had an opposite pattern of expression in ovaries and testicular interstitial cells during the disease, both on mRNA and protein level. Conclusion: Our data indicate that EAE noticeably affects the regulation of HPG axis. Further analyses are needed to explore the details of this phenomenon.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Experimental Autoimmune Encephalomyelitis Disturbs the Regulation of HPG Axis in Rats of Both Sexes",
pages = "293",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5972"
}

Milošević, A., Janjić, M., Lavrnja, I., Savić, D., Jakovljević, M., Peković, S.,& Bjelobaba, I.. (2019). Experimental Autoimmune Encephalomyelitis Disturbs the Regulation of HPG Axis in Rats of Both Sexes. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade : Serbian Neuroscience Society., 293.
https://hdl.handle.net/21.15107/rcub_ibiss_5972

Milošević A, Janjić M, Lavrnja I, Savić D, Jakovljević M, Peković S, Bjelobaba I. Experimental Autoimmune Encephalomyelitis Disturbs the Regulation of HPG Axis in Rats of Both Sexes. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:293.
https://hdl.handle.net/21.15107/rcub_ibiss_5972 .

Milošević, Ana, Janjić, Marija, Lavrnja, Irena, Savić, Danijela, Jakovljević, Marija, Peković, Sanja, Bjelobaba, Ivana, "Experimental Autoimmune Encephalomyelitis Disturbs the Regulation of HPG Axis in Rats of Both Sexes" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):293,
https://hdl.handle.net/21.15107/rcub_ibiss_5972 .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Janjić, Marija
AU  - Prévide, Rafael Maso
AU  - Abebe, Daniel
AU  - Kucka, Marek
AU  - Stojilković, Stanko S.
PY  - 2019
UR  - https://www.frontiersin.org/articles/10.3389/fendo.2019.00248/full
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3363
AB  - Cell-matrix interactions play important roles in pituitary development, physiology, and pathogenesis. In other tissues, a family of non-collagenous proteins, termed SIBLINGs, are known to contribute to cell-matrix interactions. Anterior pituitary gland expresses two SIBLING genes, Dmp1 (dentin matrix protein-1) and Spp1 (secreted phosphoprotein-1) encoding DMP1 and osteopontin proteins, respectively, but their expression pattern and roles in pituitary functions have not been clarified. Here we provide novel evidence supporting the conclusion that Spp1/osteopontin, like Dmp1/DMP1, are expressed in gonadotrophs in a sex- and age-specific manner. Other anterior pituitary cell types do not express these genes. In contrast to Dmp1, Spp1 expression is higher in males; in females, the expression reaches the peak during the diestrus phase of estrous cycle. In further contrast to Dmp1 and marker genes for gonadotrophs, the expression of Spp1 is not regulated by gonadotropin-releasing hormone in vivo and in vitro. However, Spp1 expression increases progressively after pituitary cell dispersion in both female and male cultures. We may speculate that gonadotrophs signal to other pituitary cell types about changes in the structure of pituitary cell-matrix network by osteopontin, a function consistent with the role of this secretory protein in postnatal tissue remodeling, extracellular matrix reorganization after injury, and tumorigenesis.
PB  - Frontiers Media S.A.
T2  - Frontiers in Endocrinology
T1  - Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs
IS  - MAR
VL  - 10
VL  - 10.3389/fendo.2019.00248
DO  - 10.3389/fendo.2019.00248
SP  - 248
ER  - 

@article{
author = "Bjelobaba, Ivana and Janjić, Marija and Prévide, Rafael Maso and Abebe, Daniel and Kucka, Marek and Stojilković, Stanko S.",
year = "2019",
abstract = "Cell-matrix interactions play important roles in pituitary development, physiology, and pathogenesis. In other tissues, a family of non-collagenous proteins, termed SIBLINGs, are known to contribute to cell-matrix interactions. Anterior pituitary gland expresses two SIBLING genes, Dmp1 (dentin matrix protein-1) and Spp1 (secreted phosphoprotein-1) encoding DMP1 and osteopontin proteins, respectively, but their expression pattern and roles in pituitary functions have not been clarified. Here we provide novel evidence supporting the conclusion that Spp1/osteopontin, like Dmp1/DMP1, are expressed in gonadotrophs in a sex- and age-specific manner. Other anterior pituitary cell types do not express these genes. In contrast to Dmp1, Spp1 expression is higher in males; in females, the expression reaches the peak during the diestrus phase of estrous cycle. In further contrast to Dmp1 and marker genes for gonadotrophs, the expression of Spp1 is not regulated by gonadotropin-releasing hormone in vivo and in vitro. However, Spp1 expression increases progressively after pituitary cell dispersion in both female and male cultures. We may speculate that gonadotrophs signal to other pituitary cell types about changes in the structure of pituitary cell-matrix network by osteopontin, a function consistent with the role of this secretory protein in postnatal tissue remodeling, extracellular matrix reorganization after injury, and tumorigenesis.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Endocrinology",
title = "Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs",
number = "MAR",
volume = "10, 10.3389/fendo.2019.00248",
doi = "10.3389/fendo.2019.00248",
pages = "248"
}

Bjelobaba, I., Janjić, M., Prévide, R. M., Abebe, D., Kucka, M.,& Stojilković, S. S.. (2019). Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs. in Frontiers in Endocrinology
Frontiers Media S.A.., 10(MAR), 248.
https://doi.org/10.3389/fendo.2019.00248

Bjelobaba I, Janjić M, Prévide RM, Abebe D, Kucka M, Stojilković SS. Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs. in Frontiers in Endocrinology. 2019;10(MAR):248.
doi:10.3389/fendo.2019.00248 .

Bjelobaba, Ivana, Janjić, Marija, Prévide, Rafael Maso, Abebe, Daniel, Kucka, Marek, Stojilković, Stanko S., "Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs" in Frontiers in Endocrinology, 10, no. MAR (2019):248,
https://doi.org/10.3389/fendo.2019.00248 . .

TY  - JOUR
AU  - Janjić, Marija
AU  - Prévide, Rafael M.
AU  - Fletcher, Patrick A.
AU  - Sherman, Arthur
AU  - Smiljanić, Kosara
AU  - Abebe, Daniel
AU  - Bjelobaba, Ivana
AU  - Stojilković, Stanko S.
PY  - 2019
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6934515
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3595
AB  - Continuous, as opposed to pulsatile, delivery of hypothalamic gonadotropin-releasing hormone (GnRH) leads to a marked decrease in secretion of pituitary gonadotropins LH and FSH and impairment of reproductive function. Here we studied the expression profile of gonadotropin subunit and GnRH receptor genes in rat pituitary in vitro and in vivo to clarify their expression profiles in the absence and continuous presence of GnRH. Culturing of pituitary cells in GnRH-free conditions downregulated Fshb, Cga, and Gnrhr expression, whereas continuous treatment with GnRH agonists upregulated Cga expression progressively and Gnrhr and Fshb expression transiently, accompanied by a prolonged blockade of Fshb but not Gnrhr expression. In contrast, Lhb expression was relatively insensitive to loss of endogenous GnRH and continuous treatment with GnRH, probably reflecting the status of Egr1 and Nr5a1 expression. Similar patterns of responses were observed in vivo after administration of a GnRH agonist. However, continuous treatment with GnRH stimulated LH secretion in vitro and in vivo, leading to decrease in LH cell content despite high basal Lhb expression. These data suggest that blockade of Fshb expression and depletion of the LH secretory pool are two major factors accounting for weakening of the gonadotroph secretory function during continuous GnRH treatment.
T2  - Scientific Reports
T1  - Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.
IS  - 1
VL  - 9
DO  - 10.1038/s41598-019-56480-1
SP  - 20098
ER  - 

@article{
author = "Janjić, Marija and Prévide, Rafael M. and Fletcher, Patrick A. and Sherman, Arthur and Smiljanić, Kosara and Abebe, Daniel and Bjelobaba, Ivana and Stojilković, Stanko S.",
year = "2019",
abstract = "Continuous, as opposed to pulsatile, delivery of hypothalamic gonadotropin-releasing hormone (GnRH) leads to a marked decrease in secretion of pituitary gonadotropins LH and FSH and impairment of reproductive function. Here we studied the expression profile of gonadotropin subunit and GnRH receptor genes in rat pituitary in vitro and in vivo to clarify their expression profiles in the absence and continuous presence of GnRH. Culturing of pituitary cells in GnRH-free conditions downregulated Fshb, Cga, and Gnrhr expression, whereas continuous treatment with GnRH agonists upregulated Cga expression progressively and Gnrhr and Fshb expression transiently, accompanied by a prolonged blockade of Fshb but not Gnrhr expression. In contrast, Lhb expression was relatively insensitive to loss of endogenous GnRH and continuous treatment with GnRH, probably reflecting the status of Egr1 and Nr5a1 expression. Similar patterns of responses were observed in vivo after administration of a GnRH agonist. However, continuous treatment with GnRH stimulated LH secretion in vitro and in vivo, leading to decrease in LH cell content despite high basal Lhb expression. These data suggest that blockade of Fshb expression and depletion of the LH secretory pool are two major factors accounting for weakening of the gonadotroph secretory function during continuous GnRH treatment.",
journal = "Scientific Reports",
title = "Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.",
number = "1",
volume = "9",
doi = "10.1038/s41598-019-56480-1",
pages = "20098"
}

Janjić, M., Prévide, R. M., Fletcher, P. A., Sherman, A., Smiljanić, K., Abebe, D., Bjelobaba, I.,& Stojilković, S. S.. (2019). Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.. in Scientific Reports, 9(1), 20098.
https://doi.org/10.1038/s41598-019-56480-1

Janjić M, Prévide RM, Fletcher PA, Sherman A, Smiljanić K, Abebe D, Bjelobaba I, Stojilković SS. Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.. in Scientific Reports. 2019;9(1):20098.
doi:10.1038/s41598-019-56480-1 .

Janjić, Marija, Prévide, Rafael M., Fletcher, Patrick A., Sherman, Arthur, Smiljanić, Kosara, Abebe, Daniel, Bjelobaba, Ivana, Stojilković, Stanko S., "Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo." in Scientific Reports, 9, no. 1 (2019):20098,
https://doi.org/10.1038/s41598-019-56480-1 . .

TY  - JOUR
AU  - Ehmedah, Adil
AU  - Nedeljković, Predrag
AU  - Dacic, Sanja
AU  - Repac, Jelena
AU  - Drašković Pavlović, Biljana
AU  - Vučević, Dragana
AU  - Peković, Sanja
AU  - Božić Nedeljković, Biljana
PY  - 2019
UR  - https://www.mdpi.com/1420-3049/24/24/4615
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3578
AB  - Peripheral nerve injury (PNI) leads to a series of cellular and molecular events necessary for axon regeneration and reinnervation of target tissues, among which inflammation is crucial for the orchestration of all these processes. Macrophage activation underlies the pathogenesis of PNI and is characterized by morphological/phenotype transformation from proinflammatory (M1) to an anti-inflammatory (M2) type with different functions in the inflammatory and reparative process. The aim of this study was to evaluate influence of the vitamin B (B1, B2, B3, B5, B6, and B12) complex on the process of neuroinflammation that is in part regulated by l-type CaV1.2 calcium channels. A controlled transection of the motor branch of the femoral peripheral nerve was used as an experimental model. Animals were sacrificed after 1, 3, 7, and 14 injections of vitamin B complex. Isolated nerves were used for immunofluorescence analysis. Treatment with vitamin B complex decreased expression of proinflammatory and increased expression of anti-inflammatory cytokines, thus contributing to the resolution of neuroinflammation. In parallel, B vitamins decreased the number of M1 macrophages that expressed the CaV1.2 channel, and increased the number of M2 macrophages that expressed this channel, suggesting their role in M1/M2 transition after PNI. In conclusion, B vitamins had the potential for treatment of neuroinflammation and neuroregeneration and thereby might be an effective therapy for PNI in humans.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Molecules (Basel, Switzerland)
T1  - Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.
IS  - 24
VL  - 24
DO  - 10.3390/molecules24244615
SP  - 4615
ER  - 

@article{
author = "Ehmedah, Adil and Nedeljković, Predrag and Dacic, Sanja and Repac, Jelena and Drašković Pavlović, Biljana and Vučević, Dragana and Peković, Sanja and Božić Nedeljković, Biljana",
year = "2019",
abstract = "Peripheral nerve injury (PNI) leads to a series of cellular and molecular events necessary for axon regeneration and reinnervation of target tissues, among which inflammation is crucial for the orchestration of all these processes. Macrophage activation underlies the pathogenesis of PNI and is characterized by morphological/phenotype transformation from proinflammatory (M1) to an anti-inflammatory (M2) type with different functions in the inflammatory and reparative process. The aim of this study was to evaluate influence of the vitamin B (B1, B2, B3, B5, B6, and B12) complex on the process of neuroinflammation that is in part regulated by l-type CaV1.2 calcium channels. A controlled transection of the motor branch of the femoral peripheral nerve was used as an experimental model. Animals were sacrificed after 1, 3, 7, and 14 injections of vitamin B complex. Isolated nerves were used for immunofluorescence analysis. Treatment with vitamin B complex decreased expression of proinflammatory and increased expression of anti-inflammatory cytokines, thus contributing to the resolution of neuroinflammation. In parallel, B vitamins decreased the number of M1 macrophages that expressed the CaV1.2 channel, and increased the number of M2 macrophages that expressed this channel, suggesting their role in M1/M2 transition after PNI. In conclusion, B vitamins had the potential for treatment of neuroinflammation and neuroregeneration and thereby might be an effective therapy for PNI in humans.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Molecules (Basel, Switzerland)",
title = "Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.",
number = "24",
volume = "24",
doi = "10.3390/molecules24244615",
pages = "4615"
}

Ehmedah, A., Nedeljković, P., Dacic, S., Repac, J., Drašković Pavlović, B., Vučević, D., Peković, S.,& Božić Nedeljković, B.. (2019). Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.. in Molecules (Basel, Switzerland)
Multidisciplinary Digital Publishing Institute., 24(24), 4615.
https://doi.org/10.3390/molecules24244615

Ehmedah A, Nedeljković P, Dacic S, Repac J, Drašković Pavlović B, Vučević D, Peković S, Božić Nedeljković B. Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.. in Molecules (Basel, Switzerland). 2019;24(24):4615.
doi:10.3390/molecules24244615 .

Ehmedah, Adil, Nedeljković, Predrag, Dacic, Sanja, Repac, Jelena, Drašković Pavlović, Biljana, Vučević, Dragana, Peković, Sanja, Božić Nedeljković, Biljana, "Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury." in Molecules (Basel, Switzerland), 24, no. 24 (2019):4615,
https://doi.org/10.3390/molecules24244615 . .

TY  - JOUR
AU  - Jakovljević, Marija
AU  - Lavrnja, Irena
AU  - Božić, Iva
AU  - Milošević, Ana
AU  - Bjelobaba, Ivana
AU  - Savić, Danijela
AU  - Sévigny, Jean
AU  - Peković, Sanja
AU  - Nedeljković, Nadežda
AU  - Laketa, Danijela
PY  - 2019
UR  - https://www.frontiersin.org/article/10.3389/fnins.2019.00410/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6498900
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3434
AB  - Purinergic signaling is critically involved in neuroinflammation associated with multiple sclerosis (MS) and its major inflammatory animal model, experimental autoimmune encephalomyelitis (EAE). Herein, we explored the expression of ectonucleoside triphosphate diphosphohydrolase1 (NTPDase1/CD39) in the spinal cord, at the onset (Eo), peak (Ep), and end (Ee) of EAE. Several-fold increase in mRNA and in NTPDase1 protein levels were observed at Eo and Ep. In situ hybridization combined with fluorescent immunohistochemistry showed that reactive microglia and infiltrated mononuclear cells mostly accounted for the observed increase. Colocalization analysis revealed that up to 80% of Iba1 immunoreactivity and ∼50% of CD68 immunoreactivity was colocalized with NTPDase1, while flow cytometric analysis revealed that ∼70% of mononuclear infiltrates were NTPDase1+ at Ep. Given the main role of NTPDase1 to degrade proinflammatory ATP, we hypothesized that the observed up-regulation of NTPDase1 may be associated with the transition between proinflammatory M1-like to neuroprotective M2-like phenotype of microglia/macrophages during EAE. Functional phenotype of reactive microglia/macrophages that overexpress NTPDase1 was assessed by multi-image colocalization analysis using iNOS and Arg1 as selective markers for M1 and M2 reactive states, respectively. At the peak of EAE NTPDase1 immunoreactivity showed much higher co-occurrence with Arg1 immunoreactivity in microglia and macrophages, compared to iNOS, implying its stronger association with M2-like reactive phenotype. Additionally, in ∼80% of CD68 positive cells NTPDase1 was coexpressed with Arg1 compared to negligible fraction coexpresing iNOS and ∼15% coexpresing both markers, additionally indicating prevalent association of NTPDase1 with M2-like microglial/macrophages phenotype at Ep. Together, our data suggest an association between NTPDase1 up-regulation by reactive microglia and infiltrated macrophages and their transition toward antiinflammatory phenotype in EAE.
T2  - Frontiers in Neuroscience
T1  - Induction of NTPDase1/CD39 by Reactive Microglia and Macrophages Is Associated With the Functional State During EAE.
VL  - 13
DO  - 10.3389/fnins.2019.00410
SP  - 410
ER  - 

@article{
author = "Jakovljević, Marija and Lavrnja, Irena and Božić, Iva and Milošević, Ana and Bjelobaba, Ivana and Savić, Danijela and Sévigny, Jean and Peković, Sanja and Nedeljković, Nadežda and Laketa, Danijela",
year = "2019",
abstract = "Purinergic signaling is critically involved in neuroinflammation associated with multiple sclerosis (MS) and its major inflammatory animal model, experimental autoimmune encephalomyelitis (EAE). Herein, we explored the expression of ectonucleoside triphosphate diphosphohydrolase1 (NTPDase1/CD39) in the spinal cord, at the onset (Eo), peak (Ep), and end (Ee) of EAE. Several-fold increase in mRNA and in NTPDase1 protein levels were observed at Eo and Ep. In situ hybridization combined with fluorescent immunohistochemistry showed that reactive microglia and infiltrated mononuclear cells mostly accounted for the observed increase. Colocalization analysis revealed that up to 80% of Iba1 immunoreactivity and ∼50% of CD68 immunoreactivity was colocalized with NTPDase1, while flow cytometric analysis revealed that ∼70% of mononuclear infiltrates were NTPDase1+ at Ep. Given the main role of NTPDase1 to degrade proinflammatory ATP, we hypothesized that the observed up-regulation of NTPDase1 may be associated with the transition between proinflammatory M1-like to neuroprotective M2-like phenotype of microglia/macrophages during EAE. Functional phenotype of reactive microglia/macrophages that overexpress NTPDase1 was assessed by multi-image colocalization analysis using iNOS and Arg1 as selective markers for M1 and M2 reactive states, respectively. At the peak of EAE NTPDase1 immunoreactivity showed much higher co-occurrence with Arg1 immunoreactivity in microglia and macrophages, compared to iNOS, implying its stronger association with M2-like reactive phenotype. Additionally, in ∼80% of CD68 positive cells NTPDase1 was coexpressed with Arg1 compared to negligible fraction coexpresing iNOS and ∼15% coexpresing both markers, additionally indicating prevalent association of NTPDase1 with M2-like microglial/macrophages phenotype at Ep. Together, our data suggest an association between NTPDase1 up-regulation by reactive microglia and infiltrated macrophages and their transition toward antiinflammatory phenotype in EAE.",
journal = "Frontiers in Neuroscience",
title = "Induction of NTPDase1/CD39 by Reactive Microglia and Macrophages Is Associated With the Functional State During EAE.",
volume = "13",
doi = "10.3389/fnins.2019.00410",
pages = "410"
}

Jakovljević, M., Lavrnja, I., Božić, I., Milošević, A., Bjelobaba, I., Savić, D., Sévigny, J., Peković, S., Nedeljković, N.,& Laketa, D.. (2019). Induction of NTPDase1/CD39 by Reactive Microglia and Macrophages Is Associated With the Functional State During EAE.. in Frontiers in Neuroscience, 13, 410.
https://doi.org/10.3389/fnins.2019.00410

Jakovljević M, Lavrnja I, Božić I, Milošević A, Bjelobaba I, Savić D, Sévigny J, Peković S, Nedeljković N, Laketa D. Induction of NTPDase1/CD39 by Reactive Microglia and Macrophages Is Associated With the Functional State During EAE.. in Frontiers in Neuroscience. 2019;13:410.
doi:10.3389/fnins.2019.00410 .

Jakovljević, Marija, Lavrnja, Irena, Božić, Iva, Milošević, Ana, Bjelobaba, Ivana, Savić, Danijela, Sévigny, Jean, Peković, Sanja, Nedeljković, Nadežda, Laketa, Danijela, "Induction of NTPDase1/CD39 by Reactive Microglia and Macrophages Is Associated With the Functional State During EAE." in Frontiers in Neuroscience, 13 (2019):410,
https://doi.org/10.3389/fnins.2019.00410 . .

TY  - JOUR
AU  - Dejanović, Bratislav
AU  - Vuković-Dejanović, Vesna
AU  - Ninković, Milica
AU  - Lavrnja, Irena
AU  - Stojanović, Ivana
AU  - Pavlović, Miloš
AU  - Begović, Vesna
AU  - Mirković, Duško
AU  - Stevanović, Ivana
PY  - 2018
UR  - https://actavet.vfu.cz/87/2/0145/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3097
AB  - This study was aimed to study the potentially beneficial effects of agmatine on oxidative/nitrosative stress development in the brain of Wistar rats during subacute chlorpromazine treatment. The animals were divided into control (0.9% saline), chlorpromazine (38.7 mg/kg b.w.), chlorpromazine+agmatine (agmatine 75 mg/kg b.w. immediately after chlorpromazine, 38.7 mg/kg b.w. i.p.) and agmatine (75 mg/kg b.w.) groups. All the tested substances were administered intraperitoneally for 15 consecutive days and the rats were sacrificed by decapitation on day 15. Subacute administration of chlorpromazine resulted in increased lipid peroxidation, nitric oxide concentration and superoxide anion production, while completely damaging the antioxidant defence system in the cerebral cortex, striatum, and hippocampus. However, the combined treatment with chlorpromazine and agmatine significantly attenuated the oxidative/nitrosative stress indices and restored the antioxidant capacity to the control values in all of the examined brain regions. Western blot analysis supported biochemical findings in all groups, but the most notable changes were found in the hippocampus. Our results suggest potentially beneficial effects of agmatine, which may be useful in the modified antioxidant approach in chlorpromazine-therapy.
T2  - Acta Veterinaria Brno
T1  - Effects of agmatine on chlorpromazine-induced neuronal injury in rat
IS  - 2
VL  - 87
DO  - 10.2754/avb201887020145
DO  - 0001-7213
SP  - 145
EP  - 153
ER  - 

@article{
author = "Dejanović, Bratislav and Vuković-Dejanović, Vesna and Ninković, Milica and Lavrnja, Irena and Stojanović, Ivana and Pavlović, Miloš and Begović, Vesna and Mirković, Duško and Stevanović, Ivana",
year = "2018",
abstract = "This study was aimed to study the potentially beneficial effects of agmatine on oxidative/nitrosative stress development in the brain of Wistar rats during subacute chlorpromazine treatment. The animals were divided into control (0.9% saline), chlorpromazine (38.7 mg/kg b.w.), chlorpromazine+agmatine (agmatine 75 mg/kg b.w. immediately after chlorpromazine, 38.7 mg/kg b.w. i.p.) and agmatine (75 mg/kg b.w.) groups. All the tested substances were administered intraperitoneally for 15 consecutive days and the rats were sacrificed by decapitation on day 15. Subacute administration of chlorpromazine resulted in increased lipid peroxidation, nitric oxide concentration and superoxide anion production, while completely damaging the antioxidant defence system in the cerebral cortex, striatum, and hippocampus. However, the combined treatment with chlorpromazine and agmatine significantly attenuated the oxidative/nitrosative stress indices and restored the antioxidant capacity to the control values in all of the examined brain regions. Western blot analysis supported biochemical findings in all groups, but the most notable changes were found in the hippocampus. Our results suggest potentially beneficial effects of agmatine, which may be useful in the modified antioxidant approach in chlorpromazine-therapy.",
journal = "Acta Veterinaria Brno",
title = "Effects of agmatine on chlorpromazine-induced neuronal injury in rat",
number = "2",
volume = "87",
doi = "10.2754/avb201887020145, 0001-7213",
pages = "145-153"
}

Dejanović, B., Vuković-Dejanović, V., Ninković, M., Lavrnja, I., Stojanović, I., Pavlović, M., Begović, V., Mirković, D.,& Stevanović, I.. (2018). Effects of agmatine on chlorpromazine-induced neuronal injury in rat. in Acta Veterinaria Brno, 87(2), 145-153.
https://doi.org/10.2754/avb201887020145

Dejanović B, Vuković-Dejanović V, Ninković M, Lavrnja I, Stojanović I, Pavlović M, Begović V, Mirković D, Stevanović I. Effects of agmatine on chlorpromazine-induced neuronal injury in rat. in Acta Veterinaria Brno. 2018;87(2):145-153.
doi:10.2754/avb201887020145 .

Dejanović, Bratislav, Vuković-Dejanović, Vesna, Ninković, Milica, Lavrnja, Irena, Stojanović, Ivana, Pavlović, Miloš, Begović, Vesna, Mirković, Duško, Stevanović, Ivana, "Effects of agmatine on chlorpromazine-induced neuronal injury in rat" in Acta Veterinaria Brno, 87, no. 2 (2018):145-153,
https://doi.org/10.2754/avb201887020145 . .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Begović-Kuprešanin, Vesna
AU  - Peković, Sanja
AU  - Lavrnja, Irena
PY  - 2018
UR  - http://doi.wiley.com/10.1002/jnr.24224
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3010
AB  - Multiple sclerosis (MS) is a chronic, progressive disorder of the central nervous system (CNS) that affects more than two million people worldwide. Several animal models resemble MS pathology; the most employed are experimental autoimmune encephalomyelitis (EAE) and toxin- and/or virus-induced demyelination. In this review we will summarize our knowledge on the utility of different animal models in MS research. Although animal models cannot replicate the complexity and heterogeneity of the MS pathology, they have proved to be useful for the development of several drugs approved for treatment of MS patients. This review focuses on EAE because it represents both clinical and pathological features of MS. During the past decades, EAE has been effective in illuminating various pathological processes that occur during MS, including inflammation, CNS penetration, demyelination, axonopathy, and neuron loss mediated by immune cells.
T2  - Journal of Neuroscience Research
T1  - Animal models of multiple sclerosis: Focus on experimental autoimmune encephalomyelitis.
IS  - 6
VL  - 96
DO  - 10.1002/jnr.24224
SP  - 1021
EP  - 1042
ER  - 

@article{
author = "Bjelobaba, Ivana and Begović-Kuprešanin, Vesna and Peković, Sanja and Lavrnja, Irena",
year = "2018",
abstract = "Multiple sclerosis (MS) is a chronic, progressive disorder of the central nervous system (CNS) that affects more than two million people worldwide. Several animal models resemble MS pathology; the most employed are experimental autoimmune encephalomyelitis (EAE) and toxin- and/or virus-induced demyelination. In this review we will summarize our knowledge on the utility of different animal models in MS research. Although animal models cannot replicate the complexity and heterogeneity of the MS pathology, they have proved to be useful for the development of several drugs approved for treatment of MS patients. This review focuses on EAE because it represents both clinical and pathological features of MS. During the past decades, EAE has been effective in illuminating various pathological processes that occur during MS, including inflammation, CNS penetration, demyelination, axonopathy, and neuron loss mediated by immune cells.",
journal = "Journal of Neuroscience Research",
title = "Animal models of multiple sclerosis: Focus on experimental autoimmune encephalomyelitis.",
number = "6",
volume = "96",
doi = "10.1002/jnr.24224",
pages = "1021-1042"
}

Bjelobaba, I., Begović-Kuprešanin, V., Peković, S.,& Lavrnja, I.. (2018). Animal models of multiple sclerosis: Focus on experimental autoimmune encephalomyelitis.. in Journal of Neuroscience Research, 96(6), 1021-1042.
https://doi.org/10.1002/jnr.24224

Bjelobaba I, Begović-Kuprešanin V, Peković S, Lavrnja I. Animal models of multiple sclerosis: Focus on experimental autoimmune encephalomyelitis.. in Journal of Neuroscience Research. 2018;96(6):1021-1042.
doi:10.1002/jnr.24224 .

Bjelobaba, Ivana, Begović-Kuprešanin, Vesna, Peković, Sanja, Lavrnja, Irena, "Animal models of multiple sclerosis: Focus on experimental autoimmune encephalomyelitis." in Journal of Neuroscience Research, 96, no. 6 (2018):1021-1042,
https://doi.org/10.1002/jnr.24224 . .

TY  - CONF
AU  - Savić, Danijela
AU  - Lavrnja, Irena
AU  - Bjelobaba, Ivana
AU  - Dacić, Sanja
AU  - Laketa, Danijela
AU  - Božić, Iva
AU  - Jakovljević, Marija
AU  - Nedeljković, Nadežda
AU  - Rakić, Ljubisav
AU  - Peković, Sanja
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5888
AB  - Ribavirin je purinski nukleozidni analog, otkriven pre više decenija i odobren kao lek protiv virusa hepatitisa C. Sa otkrićem direktnih antivirusnih agenasa, nastupila je revolucija u lečenju hepatitisa C, a terapijska uloga ribavirina je marginalizovana. Međutim, ribavirin ima širok spektar dejstva što je otvorilo mogućnost da se ovaj lek preusmeri ka tretmanu drugih oboljenja. Naime, osim što deluje antivirusno (inhibicija virusne RNK polimeraze i izazivanje letalne mutageneze) ribavirin je i inhibitor eukariotskog faktora za inicijaciju translacije e4E, što je zaslužno za njegov anti-tumorski efekat, pokazan u leukemiji i na ćelijama glioma. Njegova druga opšte poznata unutarćelijska meta jeste enzim inozin-5’-monofosfat dehidrogenaza (IMPDH), koji predstavlja ključni faktor u de novo sintezi guaninskih nukleotida. Ćelije koje se isključivo na ovaj način snabdevaju purinskim nukleotidima, kao što su aktivirani limfociti i neke proliferišuće ćelije, izuzetno su senzitivne na delovanje ribavirina. Inhibicija IMPDH odgovorna je za imunosupresivno i imunomodulatorno dejstvo ribavirina, pokazano u in vitro i in vivo modelima neuroinflamacije. Dakle, iako ribavirin gubi centralnu ulogu koju je imao u terapiji infekcije virusom hepatitisa C, njegova multipotentna priroda koja se ogleda u različitim mehanizmima delovanja, predstavlja potencijal za preusmeravanje ka novim terapijskim indikacijama, kao što su kancer ili multipla skleroza.
PB  - Belgrade: Serbian Biological Society
C3  - Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
T1  - Preusmeravanje antivirusnog leka ribavirina ka novim terapijskim indikacijama: primer multiple skleroze i neoplastičnih transformacija
SP  - 146
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5888
ER  - 

@conference{
author = "Savić, Danijela and Lavrnja, Irena and Bjelobaba, Ivana and Dacić, Sanja and Laketa, Danijela and Božić, Iva and Jakovljević, Marija and Nedeljković, Nadežda and Rakić, Ljubisav and Peković, Sanja",
year = "2018",
abstract = "Ribavirin je purinski nukleozidni analog, otkriven pre više decenija i odobren kao lek protiv virusa hepatitisa C. Sa otkrićem direktnih antivirusnih agenasa, nastupila je revolucija u lečenju hepatitisa C, a terapijska uloga ribavirina je marginalizovana. Međutim, ribavirin ima širok spektar dejstva što je otvorilo mogućnost da se ovaj lek preusmeri ka tretmanu drugih oboljenja. Naime, osim što deluje antivirusno (inhibicija virusne RNK polimeraze i izazivanje letalne mutageneze) ribavirin je i inhibitor eukariotskog faktora za inicijaciju translacije e4E, što je zaslužno za njegov anti-tumorski efekat, pokazan u leukemiji i na ćelijama glioma. Njegova druga opšte poznata unutarćelijska meta jeste enzim inozin-5’-monofosfat dehidrogenaza (IMPDH), koji predstavlja ključni faktor u de novo sintezi guaninskih nukleotida. Ćelije koje se isključivo na ovaj način snabdevaju purinskim nukleotidima, kao što su aktivirani limfociti i neke proliferišuće ćelije, izuzetno su senzitivne na delovanje ribavirina. Inhibicija IMPDH odgovorna je za imunosupresivno i imunomodulatorno dejstvo ribavirina, pokazano u in vitro i in vivo modelima neuroinflamacije. Dakle, iako ribavirin gubi centralnu ulogu koju je imao u terapiji infekcije virusom hepatitisa C, njegova multipotentna priroda koja se ogleda u različitim mehanizmima delovanja, predstavlja potencijal za preusmeravanje ka novim terapijskim indikacijama, kao što su kancer ili multipla skleroza.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija",
title = "Preusmeravanje antivirusnog leka ribavirina ka novim terapijskim indikacijama: primer multiple skleroze i neoplastičnih transformacija",
pages = "146",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5888"
}

Savić, D., Lavrnja, I., Bjelobaba, I., Dacić, S., Laketa, D., Božić, I., Jakovljević, M., Nedeljković, N., Rakić, L.,& Peković, S.. (2018). Preusmeravanje antivirusnog leka ribavirina ka novim terapijskim indikacijama: primer multiple skleroze i neoplastičnih transformacija. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
Belgrade: Serbian Biological Society., 146.
https://hdl.handle.net/21.15107/rcub_ibiss_5888

Savić D, Lavrnja I, Bjelobaba I, Dacić S, Laketa D, Božić I, Jakovljević M, Nedeljković N, Rakić L, Peković S. Preusmeravanje antivirusnog leka ribavirina ka novim terapijskim indikacijama: primer multiple skleroze i neoplastičnih transformacija. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija. 2018;:146.
https://hdl.handle.net/21.15107/rcub_ibiss_5888 .

Savić, Danijela, Lavrnja, Irena, Bjelobaba, Ivana, Dacić, Sanja, Laketa, Danijela, Božić, Iva, Jakovljević, Marija, Nedeljković, Nadežda, Rakić, Ljubisav, Peković, Sanja, "Preusmeravanje antivirusnog leka ribavirina ka novim terapijskim indikacijama: primer multiple skleroze i neoplastičnih transformacija" in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija (2018):146,
https://hdl.handle.net/21.15107/rcub_ibiss_5888 .

TY  - CONF
AU  - Savić, Danijela
AU  - Opačić, Miloš
AU  - Ristić, Aleksandar
AU  - Sokić, Dragoslav
AU  - Baščarević, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Živin, Marko
AU  - Šelih, Vid Simon
AU  - Spasić, Snežana
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5887
AB  - The accumulating evidence on the relation between the disturbed metal homeostasis
and epilepsy urges the need for data regarding the total metal concentrations, as well
as metal distribution in the brain itself, in order to indicate where to direct the
potential therapy, to metal supplementation or chelation. This paper summarizes our
results on the measurements of some important essential metals in hippocampi of
patients with mesial temporal lobe epilepsy (mTLE) who underwent
amigdalohippocampectomy. The key findings point out that levels of copper and
manganese are deficient in hippocampi of mTLE patients, and that their
concentrations correlated positively with neuronal loss in affected regions of sclerotic
hippocampus. In addition, the Cu concentration was decreased in the areas of total
neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor
deficient compared to control. Our results contribute to deeper insight of metals
biology in the epilepsy and may represent the initial point of new and non-invasive
therapy of drug resistant epilepsy.
PB  - Belgrade: Faculty of Chemistry: Serbian Biochemical Society
C3  - Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia
T1  - Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy
SP  - 105
EP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5887
ER  - 

@conference{
author = "Savić, Danijela and Opačić, Miloš and Ristić, Aleksandar and Sokić, Dragoslav and Baščarević, Vladimir and Raičević, Savo and Savić, Slobodan and Živin, Marko and Šelih, Vid Simon and Spasić, Snežana and Spasojević, Ivan",
year = "2018",
abstract = "The accumulating evidence on the relation between the disturbed metal homeostasis
and epilepsy urges the need for data regarding the total metal concentrations, as well
as metal distribution in the brain itself, in order to indicate where to direct the
potential therapy, to metal supplementation or chelation. This paper summarizes our
results on the measurements of some important essential metals in hippocampi of
patients with mesial temporal lobe epilepsy (mTLE) who underwent
amigdalohippocampectomy. The key findings point out that levels of copper and
manganese are deficient in hippocampi of mTLE patients, and that their
concentrations correlated positively with neuronal loss in affected regions of sclerotic
hippocampus. In addition, the Cu concentration was decreased in the areas of total
neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor
deficient compared to control. Our results contribute to deeper insight of metals
biology in the epilepsy and may represent the initial point of new and non-invasive
therapy of drug resistant epilepsy.",
publisher = "Belgrade: Faculty of Chemistry: Serbian Biochemical Society",
journal = "Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia",
title = "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy",
pages = "105-112",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5887"
}

Savić, D., Opačić, M., Ristić, A., Sokić, D., Baščarević, V., Raičević, S., Savić, S., Živin, M., Šelih, V. S., Spasić, S.,& Spasojević, I.. (2018). Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia
Belgrade: Faculty of Chemistry: Serbian Biochemical Society., 105-112.
https://hdl.handle.net/21.15107/rcub_ibiss_5887

Savić D, Opačić M, Ristić A, Sokić D, Baščarević V, Raičević S, Savić S, Živin M, Šelih VS, Spasić S, Spasojević I. Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia. 2018;:105-112.
https://hdl.handle.net/21.15107/rcub_ibiss_5887 .

Savić, Danijela, Opačić, Miloš, Ristić, Aleksandar, Sokić, Dragoslav, Baščarević, Vladimir, Raičević, Savo, Savić, Slobodan, Živin, Marko, Šelih, Vid Simon, Spasić, Snežana, Spasojević, Ivan, "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy" in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia (2018):105-112,
https://hdl.handle.net/21.15107/rcub_ibiss_5887 .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Li, Shuo
AU  - Stojilković, Stanko S.
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0005273617301098
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2745
AB  - Pannexins are a three-member family of vertebrate plasma membrane spanning molecules that have homology to the invertebrate gap junction forming proteins, the innexins. However, pannexins do not form gap junctions but operate as plasma membrane channels. The best-characterized member of these proteins, Pannexin1 (Panx1) was suggested to be functionally associated with purinergic P2X and N-methyl-D-aspartate (NMDA) receptor channels. Activation of these receptor channels by their endogenous ligands leads to cross-activation of Panx1 channels. This in turn potentiates P2X and NMDA receptor channel signaling. Two potentiation concepts have been suggested: enhancement of the current responses and/or sustained receptor channel activation by ATP released through Panx1 pore and adenosine generated by ectonucleotidase-dependent dephosphorylation of ATP. Here we summarize the current knowledge and hypotheses about interactions of Panx1 channels with P2X and NMDA receptor channels. This article is part of a Special Issue entitled: Gap Junction Proteins edited by Jean Claude Herve.
T2  - Biochimica et Biophysica Acta (BBA) - Biomembranes
T1  - Interactions of Pannexin1 channels with purinergic and NMDA receptor channels
IS  - 1
VL  - 1860
DO  - 10.1016/j.bbamem.2017.03.025
SP  - 166
EP  - 173
ER  - 

@article{
author = "Bjelobaba, Ivana and Li, Shuo and Stojilković, Stanko S.",
year = "2018",
abstract = "Pannexins are a three-member family of vertebrate plasma membrane spanning molecules that have homology to the invertebrate gap junction forming proteins, the innexins. However, pannexins do not form gap junctions but operate as plasma membrane channels. The best-characterized member of these proteins, Pannexin1 (Panx1) was suggested to be functionally associated with purinergic P2X and N-methyl-D-aspartate (NMDA) receptor channels. Activation of these receptor channels by their endogenous ligands leads to cross-activation of Panx1 channels. This in turn potentiates P2X and NMDA receptor channel signaling. Two potentiation concepts have been suggested: enhancement of the current responses and/or sustained receptor channel activation by ATP released through Panx1 pore and adenosine generated by ectonucleotidase-dependent dephosphorylation of ATP. Here we summarize the current knowledge and hypotheses about interactions of Panx1 channels with P2X and NMDA receptor channels. This article is part of a Special Issue entitled: Gap Junction Proteins edited by Jean Claude Herve.",
journal = "Biochimica et Biophysica Acta (BBA) - Biomembranes",
title = "Interactions of Pannexin1 channels with purinergic and NMDA receptor channels",
number = "1",
volume = "1860",
doi = "10.1016/j.bbamem.2017.03.025",
pages = "166-173"
}

Bjelobaba, I., Li, S.,& Stojilković, S. S.. (2018). Interactions of Pannexin1 channels with purinergic and NMDA receptor channels. in Biochimica et Biophysica Acta (BBA) - Biomembranes, 1860(1), 166-173.
https://doi.org/10.1016/j.bbamem.2017.03.025

Bjelobaba I, Li S, Stojilković SS. Interactions of Pannexin1 channels with purinergic and NMDA receptor channels. in Biochimica et Biophysica Acta (BBA) - Biomembranes. 2018;1860(1):166-173.
doi:10.1016/j.bbamem.2017.03.025 .

Bjelobaba, Ivana, Li, Shuo, Stojilković, Stanko S., "Interactions of Pannexin1 channels with purinergic and NMDA receptor channels" in Biochimica et Biophysica Acta (BBA) - Biomembranes, 1860, no. 1 (2018):166-173,
https://doi.org/10.1016/j.bbamem.2017.03.025 . .