TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Trifunović, Svetlana
AU  - Jarić, Ivana
AU  - Ristić, Nataša
AU  - Milošević, Verica
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6007
AB  - We previously reported that Vitex agnus-castus L. essential oil (VACEO), when administered to middle-aged males, exerts a bone-protective effect, induces silencing of locomotor activities and decreases pituitary prolactin immunopositivity. To further assess the putative endocrine effects of VACEO, we examined the pituitary-thyroid and -adrenocortical axes in our model. Sixteen-month-old Wistar rats were subcutaneously administered 60mg/kg of VACEO dissolved in sterile olive oil, while the control group received the same amount of vehicle alone for three weeks. Pituitaries, thyroids and adrenals were analyzed by qualitative and quantitative histological approaches. Concentration of thyroid stimulating hormone (TSH), total thyroxine and triiodothyronine (TH), adrenocorticotrophic hormone (ACTH), corticosterone in serum and in adrenal tissue were measured. In VACEO-treated rats, the relative volume density of pituitary thyrotrophs increased (p<0.001), while intensity of cytoplasmic TSHβ immunostaining decreased (p<0.001), consistent with elevated TSH in serum (p<0.01). The thyroid tissue was characterized by a micro-follicular structure, increased relative volume of follicular epithelium (p<0.05), decreased volume of luminal colloid (p<0.001) and increased basolateral expression of sodium-iodide symporter-immunopositivity (p<0.05). Serum TH also increased (p<0.01). The relative volume density of pituitary corticotrophs decreased (p<0.05), compatible with decline in circulating ACTH (p<0.05). Neither tissue nor serum corticosterone levels were affected by VACEO treatment. In conclusion, the observed changes in TSH and ACTH strongly indicate central endocrine effects of prolonged VACEO treatment. In this respect, production of ACTH decreased without impact on corticosterone production. Increase in serum concentration of both TH and TSH are not compatible with a negative feedback loop and suggest a major change in set-point regulation of the hypothalamic-pituitary-thyroid axis.
PB  - Elsevier
T2  - Acta Histochemica
T1  - Functional morphology of pituitary -thyroid and -adrenocortical axes in middle-aged male rats treated with Vitex agnus castus essential oil
IS  - 7
VL  - 118
DO  - 10.1016/j.acthis.2016.07.007
SP  - 736
EP  - 745
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Ajdžanović, Vladimir and Filipović, Branko and Trifunović, Svetlana and Jarić, Ivana and Ristić, Nataša and Milošević, Verica",
year = "2016",
abstract = "We previously reported that Vitex agnus-castus L. essential oil (VACEO), when administered to middle-aged males, exerts a bone-protective effect, induces silencing of locomotor activities and decreases pituitary prolactin immunopositivity. To further assess the putative endocrine effects of VACEO, we examined the pituitary-thyroid and -adrenocortical axes in our model. Sixteen-month-old Wistar rats were subcutaneously administered 60mg/kg of VACEO dissolved in sterile olive oil, while the control group received the same amount of vehicle alone for three weeks. Pituitaries, thyroids and adrenals were analyzed by qualitative and quantitative histological approaches. Concentration of thyroid stimulating hormone (TSH), total thyroxine and triiodothyronine (TH), adrenocorticotrophic hormone (ACTH), corticosterone in serum and in adrenal tissue were measured. In VACEO-treated rats, the relative volume density of pituitary thyrotrophs increased (p<0.001), while intensity of cytoplasmic TSHβ immunostaining decreased (p<0.001), consistent with elevated TSH in serum (p<0.01). The thyroid tissue was characterized by a micro-follicular structure, increased relative volume of follicular epithelium (p<0.05), decreased volume of luminal colloid (p<0.001) and increased basolateral expression of sodium-iodide symporter-immunopositivity (p<0.05). Serum TH also increased (p<0.01). The relative volume density of pituitary corticotrophs decreased (p<0.05), compatible with decline in circulating ACTH (p<0.05). Neither tissue nor serum corticosterone levels were affected by VACEO treatment. In conclusion, the observed changes in TSH and ACTH strongly indicate central endocrine effects of prolonged VACEO treatment. In this respect, production of ACTH decreased without impact on corticosterone production. Increase in serum concentration of both TH and TSH are not compatible with a negative feedback loop and suggest a major change in set-point regulation of the hypothalamic-pituitary-thyroid axis.",
publisher = "Elsevier",
journal = "Acta Histochemica",
title = "Functional morphology of pituitary -thyroid and -adrenocortical axes in middle-aged male rats treated with Vitex agnus castus essential oil",
number = "7",
volume = "118",
doi = "10.1016/j.acthis.2016.07.007",
pages = "736-745"
}

Šošić-Jurjević, B., Ajdžanović, V., Filipović, B., Trifunović, S., Jarić, I., Ristić, N.,& Milošević, V.. (2016). Functional morphology of pituitary -thyroid and -adrenocortical axes in middle-aged male rats treated with Vitex agnus castus essential oil. in Acta Histochemica
Elsevier., 118(7), 736-745.
https://doi.org/10.1016/j.acthis.2016.07.007

Šošić-Jurjević B, Ajdžanović V, Filipović B, Trifunović S, Jarić I, Ristić N, Milošević V. Functional morphology of pituitary -thyroid and -adrenocortical axes in middle-aged male rats treated with Vitex agnus castus essential oil. in Acta Histochemica. 2016;118(7):736-745.
doi:10.1016/j.acthis.2016.07.007 .

Šošić-Jurjević, Branka , Ajdžanović, Vladimir, Filipović, Branko, Trifunović, Svetlana, Jarić, Ivana, Ristić, Nataša, Milošević, Verica, "Functional morphology of pituitary -thyroid and -adrenocortical axes in middle-aged male rats treated with Vitex agnus castus essential oil" in Acta Histochemica, 118, no. 7 (2016):736-745,
https://doi.org/10.1016/j.acthis.2016.07.007 . .